Abstract

The present invention relates to a method for diagnosing rubella using recombinant proteins. The recombinant proteins developed can be used individually or together, are of high purity and have high capacity for discriminating the rubella virus. The invention also enables the development of a second-generation subunit vaccine. In general, the invention relates to a recombinant protein, a synthetic DNA sequence, an expression vector, a host cell, a composition, a kit for diagnosing rubella, the use of at least one recombinant protein and methods for producing a recombinant protein and diagnosing rubella.

IPC Classes  ?

• C07K 14/19 - Rubella virus
• A61K 39/20 - Rubella virus
• C12N 15/40 - Proteins from RNA viruses, e.g. flaviviruses
• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The present invention relates to polypeptides that have immunogenic activity, which can be used alone or together, having high discriminatory capacity for screening the hepatitis C virus (HCV). The polypeptides according to the invention comprise at least two antigenic HCV regions selected from the nucleocapsid region and nonstructural regions NS3, NS4 and NS5. The invention also relates to the nucleic acid, expression cassette, expression vector, host cell, method for producing the polypeptides, composition, use of the polypeptides, kit for immunological screening for HCV and/or for diagnosing hepatitis C, and also to the methods for immunological screening for HCV and for diagnosing hepatitis C.

IPC Classes  ?

• C07K 14/18 - Togaviridae, e.g. flavivirus, pestivirus, yellow fever virus, hepatitis C virus, japanese encephalitis virus
• C07K 19/00 - Hybrid peptides
• C07K 17/00 - Carrier-bound or immobilised peptidesPreparation thereof
• A61K 39/29 - Hepatitis virus
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/51 - Hepatitis viruses
• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• G01N 33/576 - ImmunoassayBiospecific binding assayMaterials therefor for hepatitis

Abstract

The present invention describes i) the construction of integration vectors for heterologous overexpression in combination with a system for selecting cells and for real-time monitoring of productivity by the co-expression of reporter proteins, ii) the selection of cells that secrete high levels of the protein of interest by labelling with fluorescent probes specific for the endoplasmic reticulum (ER), iii) the construction of a genetically modified CHO cell line for conditional expression of proteins of interest under tetracycline/doxycycline control (TetON system), iv) the development of a synthetic gene containing the active forms of proteins Atf6 and Xbp1 fused together in a single polypeptide and CHO cell lines expressing the Atf6-Xbp1 fusion under optional tetracycline control, v) the overexpression and production of high levels of recombinant proteins by the regulated co-expression of Atf6-Xbp1.

IPC Classes  ?

• C12N 15/67 - General methods for enhancing the expression
• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

The present invention provides a lateral flow diagnostic device with radial symmetry comprising a base (B) and a lid (T), which comprises at least one sample input opening (1) and at least two observation windows (2), and the base (B) comprises at least one sample acquisition element (3) and at least two test-strip beds (5), wherein each observation opening (2) in the lid (T) corresponds to one or more beds (5), and in each bed (5) one or more test strips (4) can be positioned, said at least one sample acquisition element (3) being in communication with the at least two test strips (4). The invention also provides a system for reading a lateral flow diagnostic device with radial symmetry comprising the following modules: image acquisition optical module (13), illumination module, processing and control module (15), and storage module, wherein the image and acquisition optical module (13) is adapted to acquire an image of a lateral flow diagnostic device with radial symmetry, and the processing and control module (15) is adapted to manage and extract information from the system.

IPC Classes  ?

• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G01N 21/84 - Systems specially adapted for particular applications
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

The present patent application discloses recombinant or synthetic T. gondii antigens that can be used for diagnosing acute and chronic toxoplasmosis. Combinations for detecting IgG anti-T. gondii antibodies and IgM anti-T. gondii antibodies are disclosed, comprising the antigens GRA7, ROP1, p35 and GRA6, or fragments thereof. In addition, the present patent application discloses nucleotide sequences encoding the antigens of the present invention, as well as systems for recombinant expression and systems for purification of said antigens. Furthermore, stabilized compositions comprising the antigens of the invention are also provided, as well as said antigens coupled to microbeads.

IPC Classes  ?

• C07K 14/45 - Toxoplasma
• C12N 15/30 - Genes encoding protozoal proteins, e.g. from Plasmodium, Trypanosoma, Eimeria
• A61K 39/002 - Protozoa antigens
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07K 17/08 - Peptides being immobilised on, or in, an organic carrier the carrier being a synthetic polymer

Abstract

The present invention describes i) the construction of synthetic genes for components of a recombinant fibrin glue: fibrinogen A, fibrinogen B, fibrinogen G, factor XIIIA, prothrombin, ecarin and ERp57; ii) the expression of said genes in genetically modified CHO cells to achieve high levels of expression; iii) the increase in fibrinogen expression by the coexpression of ERp57; iv) the scaling of said expression system in a bioreactor; v) the purification of the glue components using chromatographic methods; vi) the formulation of the glue components for use as a surgical adjunct.

IPC Classes  ?

• A61L 24/10 - PolypeptidesProteins
• A61K 38/36 - Blood coagulation or fibrinolysis factors
• A61K 38/48 - Hydrolases (3) acting on peptide bonds (3.4)
• C12N 15/67 - General methods for enhancing the expression
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 9/64 - Proteinases derived from animal tissue, e.g. rennin
• C07K 14/745 - Blood coagulation or fibrinolysis factors
• C07K 14/75 - Fibrinogen

Abstract

The present invention relates to a process for producing polymeric structures that have activated surfaces. The process proved to be simple, quick, with high production capacity and low operating costs. The process occurs by depositing a polymer solution, which is assisted by a high electric field, on a conductive liquid surface to produce particles and/or filaments that have an activated surface. More particularly, the process of the present invention has the ability to produce particles and/or filaments that have chemically activated surfaces, in a single process.

IPC Classes  ?

• D01D 7/00 - Collecting the newly-spun products
• D01D 5/00 - Formation of filaments, threads, or the like
• B29B 9/06 - Making granules by dividing preformed material in the form of filamentary material, e.g. combined with extrusion
• B29B 9/12 - Making granules characterised by structure or composition
• D01F 1/10 - Other agents for modifying properties
• D01F 6/16 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from homopolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds from polymers of unsaturated carboxylic acids or unsaturated organic esters, e.g. polyacrylic esters, polyvinyl acetate
• D01F 6/22 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from homopolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds from polymers of cyclic compounds with one carbon-to-carbon double bond in the side chain from polystyrene