A method includes providing a system comprising an electrophoresis cartridge interface that releasably engages with an electrophoresis cartridge that includes an anode, a cathode, and at least one electrophoresis capillary that is in fluid communication with the anode and the cathode. The method further includes receiving the electrophoresis cartridge at said electrophoresis cartridge interface and automatically establishing at least one of (i) optical communication between an optical detection assembly of the system and a portion of the at least one electrophoresis capillary, (ii) electrical communication between the system and the anode and cathode, (iii) fluidic communication between the system and the at least one electrophoresis capillary, (iv) thermal communication between the system and the electrophoresis cartridge or the at least one electrophoresis capillary; (v) electromagnetic communication between the system and the electrophoresis cartridge and (vi) magnetic communication between the system and the electrophoresis cartridge.
Provided is a capillary electrophoresis device in which it is easy to exchange a capillary. This capillary electrophoresis device comprises: a capillary cartridge 102 in which a capillary 101 has been positioned; a first structure 106 in which a path to which one end of the capillary is connected has been formed; and a second structure 107 in which a path to which the other end of the capillary is connected has been formed, wherein at least one among the first structure body and the second structure is moved within a predetermined range.
An instrument includes a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to close off a fluidic channel in the body. A cartridge interface is configured to engage the cartridge.
A live help provider graphical user interface depicting a provider graphical representation of a client device located at a user location and respective provider graphical representations of additional devices located at the user location in a manner that identifies each of the additional devices and depicts a spatial relationship of each of the additional devices relative to the client device at the user location.
G06F 9/451 - Execution arrangements for user interfaces
G06F 3/0487 - Interaction techniques based on graphical user interfaces [GUI] using specific features provided by the input device, e.g. functions controlled by the rotation of a mouse with dual sensing arrangements, or of the nature of the input device, e.g. tap gestures based on pressure sensed by a digitiser
A system for controlling custody of a sample can include a cartridge comprising a sample receptacle configured to receive an article configured to deliver a sample to the cartridge, a cartridge module having a cartridge receptacle configured to receive the cartridge; a housing enclosing the cartridge module and the cartridge. The housing can include a closable door; at least one sensor configured to produce a signal indicating: insertion or removal of an article into or from the sample receptacle; whether the cartridge is inserted into the cartridge receptacle; or whether the door is open or closed. The system can further include a computer programmed to record a signal from the at least one sensor for a recording period of time and to display a signal history to a user.
A method of controlling fluid flow in a fluid channel of fluidic cartridge can include closing a valve by actuating a mechanical actuator against the valve to force a deformable layer against walls of a valve body, wherein the valve body comprises a valve inlet and a valve outlet and each of the valve inlet and valve outlet are fluidically coupled to a fluidic channel; releasing the valve by retracting the mechanical actuator away from the valve body; and moving liquid through the valve by applying positive or negative pressure to the fluidic channel.
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.
Disclosed are systems and methods for improving the function of analytical instruments used to analyze dye-labelled nucleic acid samples by minimizing spectral anomalies from dye data. A computer system communicatively coupled to the instrument is configured to select multiple different nucleic acids of different sizes and determine dye spectral profiles associated with each of the different nucleic acids. The spectral profiles are used to generate multiple dye matrices each respectively associated with different nucleic acid sizes. When analyzing a test sample, the dye matrices are then separately applied to nucleic acid fragments with sizes similar to the nucleic acids from which the dye matrices were derived, better tailoring the dye matrices to the conditions in which they were generated and minimizing unwanted dye data artifacts.
G01N 21/27 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
Methods for analyzing raw electropherogram data are disclosed. Some methods includes extracting color data as a function of time or position from the raw electropherogram data, selecting from the electropherogram one or more peaks that contain color data for a first dye and substantially no color data from other dyes used in electrophoresis. The method also includes determining the color spectrum of the first dye, and using the color spectrum of the first dye to deconvolve the color data of the raw electropherogram data to separate the contributions of each of the dyes to the raw electropherogram data. Systems and apparatus for producing electropherograms are also disclosed.
A system for performing capillary electrophoresis of multiple samples comprises a capillary containing a separation medium and having inlet and distal ends and an interrogation region; a power source configured to apply voltages between inlet and distal ends; and logic to cause execution of: applying a first substantially constant forward polarity electrophoresis voltage to the capillary; before all of the first DNA fragments have passed the interrogation region, applying a reverse polarity voltage pulse to the capillary, thereby transporting at least some of the first DNA fragments in the capillary toward the capillary inlet; introducing a second sample to the capillary inlet, the second sample comprising second DNA fragments having a plurality of different sizes; and applying a second substantially constant forward polarity electrophoresis voltage to the capillary to simultaneously perform electrophoresis on the second DNA fragments and the first DNA fragments.
A system comprises an authentication sub-system configured to authenticate a user and a biochemical analysis sub-system configured to perform a biochemical analysis on a biological sample provided to the biochemical analysis sub-system. The system is configured to authenticate a user using the authentication sub-system, instruct an authenticated user to provide a biological sample to the biochemical analysis sub-system, execute a biochemical analysis on a provided biological sample.
G06V 40/70 - Multimodal biometrics, e.g. combining information from different biometric modalities
G10L 17/00 - Speaker identification or verification techniques
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
G16B 50/30 - Data warehousingComputing architectures
G06V 40/10 - Human or animal bodies, e.g. vehicle occupants or pedestriansBody parts, e.g. hands
G06V 40/60 - Static or dynamic means for assisting the user to position a body part for biometric acquisition
G16B 99/00 - Subject matter not provided for in other groups of this subclass
A live help system provides an intuitive display of help information on a user's graphical user interface. A request is received from a client device for help, and a live help provider interface is initiated at a live help location. Data is acquired regarding a user's location, including data on external devices in the user's location. Indicators are provided to allow the live help provider to point to or otherwise indicate items on the user interface or outside of the user interface. Live help input is captured at the live help provider interface. Instructions are then transmitted to the display of the client device to display live help input, as though the agent were present and interacting with or indicating items on the screen or off the screen.
G06F 3/0481 - Interaction techniques based on graphical user interfaces [GUI] based on specific properties of the displayed interaction object or a metaphor-based environment, e.g. interaction with desktop elements like windows or icons, or assisted by a cursor's changing behaviour or appearance
G06F 9/451 - Execution arrangements for user interfaces
G06F 3/0487 - Interaction techniques based on graphical user interfaces [GUI] using specific features provided by the input device, e.g. functions controlled by the rotation of a mouse with dual sensing arrangements, or of the nature of the input device, e.g. tap gestures based on pressure sensed by a digitiser
Provided herein is a system and method for review of forensic report computer files. The method can involve notifying a plurality of service providers of a job to be performed, accepting a bid from a service provider to perform the job, providing the computer file to the service provider, receiving from the service provider a forensic result such as a reviewed file.
G06Q 50/00 - Information and communication technology [ICT] specially adapted for implementation of business processes of specific business sectors, e.g. utilities or tourism
Performing sample quantitation and sample amplification may be performed in a sample cartridge or sample cartridges. Sample quantitation using qPCR may be performed during STR PCR on the sample. Samples need not be normalized prior to performing STR PCR. In certain embodiments, qPCR and STR PCR are performed on the same cartridge, optionally at the same time (or in real-time, or overlapping in time) and optionally using some or all of the same PCR apparatus. In other embodiments, qPCR and STR PCR are performed on different cartridges. Quantitation of the STR PCR sample may be performed without substantially delaying the STR PCR process.
Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.
A command center includes at least one network communications interface configured for two-way communications with a plurality of sites remote from the command center and at least one display screen and user interface. Each of the plurality of sites includes at least one forensic field test device configured to identify individuals using DNA samples from the individuals. The display screen and user interface are configured to depict aspects of forensic field test devices of the plurality of sites, wherein the aspects include a site identifier for each of the forensic field test devices and one or more additional aspects.
G06F 3/01 - Input arrangements or combined input and output arrangements for interaction between user and computer
G06F 3/04817 - Interaction techniques based on graphical user interfaces [GUI] based on specific properties of the displayed interaction object or a metaphor-based environment, e.g. interaction with desktop elements like windows or icons, or assisted by a cursor's changing behaviour or appearance using icons
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
An electrophoresis cartridge can include an integrated electrophoresis assembly including an anode sub-assembly comprising an anode, a cathode sub-assembly comprising a cathode; and at least one electrophoresis capillary having a first end in fluid communication with the cathode sub-assembly and a second end in fluid communication with the anode sub-assembly. The cartridge can further include a reagent container comprising electrophoresis buffer; a fluid conduit in fluidic communication with the reagent container; a pump in fluidic communication with the fluid conduit, the pump configured to transfer the electrophoresis buffer through the fluid conduit from the reagent container to the passage of the cathode sub-assembly; and a sample inlet port and at least one sample line, wherein the sample line places the sample inlet port in fluidic communication with the first end of the electrophoresis capillary through a passage in the cathode sub-assembly.
A live help system provides an intuitive display of help information on a user's graphical user interface. A request is received from a client device for help, and a live help provider interface is initiated at a live help location. Data is acquired regarding a user's location, including data on external devices in the user's location. Indicators are provided to allow the live help provider to point to or otherwise indicate items on the user interface or outside of the user interface. Live help input is captured at the live help provider interface. Instructions are then transmitted to the display of the client device to display live help input, as though the agent were present and interacting with or indicating items on the screen or off the screen.
G06F 3/0481 - Interaction techniques based on graphical user interfaces [GUI] based on specific properties of the displayed interaction object or a metaphor-based environment, e.g. interaction with desktop elements like windows or icons, or assisted by a cursor's changing behaviour or appearance
G06F 9/451 - Execution arrangements for user interfaces
G06F 3/0487 - Interaction techniques based on graphical user interfaces [GUI] using specific features provided by the input device, e.g. functions controlled by the rotation of a mouse with dual sensing arrangements, or of the nature of the input device, e.g. tap gestures based on pressure sensed by a digitiser
A method of making an article bonding a layer of deformable material to a surface of a cartridge body having at least one valve body thereon to seal the at least one valve body, the valve body comprising a valve floor and valve walls comprising upper parts, wherein the valve floor is recessed from the surface and the valve walls extend from the surface to the valve floor, and wherein the valve body comprises walls that are curved or sloped in a direction that is non-normal to the plane defined by the surface; and deforming the layer such that upon release of a force causing the deformation, the layer is in contact with the upper parts of the valve walls and is in spaced from the valve floor so as to bias the valve to an open state.
Performing sample quantitation and sample amplification may be performed in a sample cartridge or sample cartridges. Sample quantitation using qPCR may be performed during STR PCR on the sample. Samples need not be normalized prior to performing STR PCR. In certain embodiments, qPCR and STR PCR are performed on the same cartridge, optionally at the same time (or in real-time, or overlapping in time) and optionally using some or all of the same PCR apparatus. In other embodiments, qPCR and STR PCR are performed on different cartridges. Quantitation of the STR PCR sample may be performed without substantially delaying the STR PCR process.
Disclosed are methods for performing capillary electrophoresis on two or more nucleic acid samples. The methods employ a forward voltage to move a first sample forward from an inlet to an interrogation region in the capillary, then a backward voltage to move the first sample backward, and then a forward voltage again to move the first sample and a second sample forward. Systems and apparatuses for performing capillary electrophoresis are also provided.
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.
C08G 61/04 - Macromolecular compounds containing only carbon atoms in the main chain of the macromolecule, e.g. polyxylylenes only aliphatic carbon atoms
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.
Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.
This disclosure provides, among other things, a cartridge comprising: (a) a cartridge body comprising a malleable material and having, disposed on a surface of the body, at least one valve body comprising a valve inlet and a valve outlet, each fluidically connected to a fluidic channel; and (b) a layer comprising a deformable material bonded to a surface of the cartridge body and sealing the at least one valve body at points of attachment, thereby forming at least one valve; wherein the at least one valve body is depressed in the cartridge body relative to the points of attachment and wherein the deformable material covering the at least one valve body retains sufficient elasticity after deformation such that in a ground state the valve is open. Also disclosed is an instrument comprising a cartridge interface and a cartridge as described herein engaged with the cartridge interface, wherein (II) the cartridge interface comprises: (A) at least one mechanical actuator, each mechanical actuator positioned to actuate a valve; and (B) at least one motor operatively coupled to actuate a mechanical actuator toward or away from a valve.
A biometric biochemical analysis system includes a user interface module to provide instructions for collecting and handling biochemical sampling and processing related to biometric data gathering as well as capturing biometric data using digital data capturing devices. The user interface module and display are integrated with analysis and communications portions of the biometric biochemical analysis system to provide a portable system for multi-portion data collecting, storage, verification, and analysis.
G06V 40/70 - Multimodal biometrics, e.g. combining information from different biometric modalities
G10L 17/00 - Speaker identification or verification techniques
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
G16B 50/30 - Data warehousingComputing architectures
G06V 40/10 - Human or animal bodies, e.g. vehicle occupants or pedestriansBody parts, e.g. hands
G06V 40/60 - Static or dynamic means for assisting the user to position a body part for biometric acquisition
G16B 99/00 - Subject matter not provided for in other groups of this subclass
G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
A live help system provides an intuitive display of help information on a user's graphical user interface. A request is received from a client device for help, and a live help provider interface is initiated at a live help location. Data is acquired regarding a user's location, including data on external devices in the user's location. Indicators are provided to allow the live help provider to point to or otherwise indicate items on the user interface or outside of the user interface. Live help input is captured at the live help provider interface. Instructions are then transmitted to the display of the client device to display live help input, as though the agent were present and interacting with or indicating items on the screen or off the screen.
G06F 3/0481 - Interaction techniques based on graphical user interfaces [GUI] based on specific properties of the displayed interaction object or a metaphor-based environment, e.g. interaction with desktop elements like windows or icons, or assisted by a cursor's changing behaviour or appearance
G06F 9/451 - Execution arrangements for user interfaces
G06F 3/0487 - Interaction techniques based on graphical user interfaces [GUI] using specific features provided by the input device, e.g. functions controlled by the rotation of a mouse with dual sensing arrangements, or of the nature of the input device, e.g. tap gestures based on pressure sensed by a digitiser
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.
Disclosed are methods for performing capillary electrophoresis on two or more nucleic acid samples. The methods employ a forward voltage to move a first sample forward from an inlet to an interrogation region in the capillary, then a backward voltage to move the first sample backward, and then a forward voltage again to move the first sample and a second sample forward. Systems and apparatuses for performing capillary electrophoresis are also provided.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
35.
COMPOSITIONS, METHODS, KITS AND DEVICES FOR MOLECULAR ANALYSIS
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Provided herein is a system and method for review of forensic report computer files. The method can involve notifying a plurality of service providers of a job to be performed, accepting a bid from a service provider to perform the job, providing the computer file to the service provider, receiving from the service provider a forensic result such as a reviewed file.
G06F 16/11 - File system administration, e.g. details of archiving or snapshots
G06Q 50/00 - Information and communication technology [ICT] specially adapted for implementation of business processes of specific business sectors, e.g. utilities or tourism
A command center includes at least one network communications interface configured for two-way communications with a plurality of sites remote from the command center and at least one display screen and user interface. Each of the plurality of sites includes at least one forensic field test device configured to identify individuals using DNA samples from the individuals. The display screen and user interface are configured to depict aspects of forensic field test devices of the plurality of sites, wherein the aspects include a site identifier for each of the forensic field test devices and one or more additional aspects.
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G06F 3/01 - Input arrangements or combined input and output arrangements for interaction between user and computer
G06F 3/0481 - Interaction techniques based on graphical user interfaces [GUI] based on specific properties of the displayed interaction object or a metaphor-based environment, e.g. interaction with desktop elements like windows or icons, or assisted by a cursor's changing behaviour or appearance
Methods for analyzing raw electropherogram data are disclosed. Some methods includes extracting color data as a function of time or position from the raw electropherogram darta, selecting from the electropherogram one or more peaks that contain color data for a first dye and substantially no color data from other dyes used in electrophoresis. The method also includes determining the color spectrum of the first dye, and using the color spectrum of the first dye to deconvolve the color data of the raw electropherogram data to separate the contributions of each of the dyes to the raw electropherogram data. Systems and apparatus for producing electropherograms are also disclosed.
09 - Scientific and electric apparatus and instruments
Goods & Services
Software for laboratory equipment and instruments, namely,
fluid handling devices, apparatus to automate chemical or
biochemical reactions, thermal cyclers, capillary
electrophoresis devices, detection devices, namely,
detection of nucleic acids in biological samples, for use in
relation to scientific, medical, environmental, veterinary,
food, agriculture, supply chain management, and forensic
research, analysis, testing and diagnostics; computer
equipment and software for automating or integrating
biochemical processes or biochemical sample preparation,
namely, for processing samples for the preparation or
analysis of biomolecules; software for laboratory equipment,
namely, microfluidic devices for processing samples for the
preparation and analysis of biomolecules.
41.
Sample preparation, processing and analysis systems
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
Abiochemical analysis includes a user interface module tp provide instructions for collecting and handling biochemical sampling and processing related to biometric data gathering as well as caturing biometric data using digital data capturing devices. The user interface module and display are intergrated with analysis and communications portions of the biometric biochemical analysis system to provide a portable system for multi-portion data collecting, stroage, verification and analysis.
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
A biometric biochemical analysis system includes a user interface module to provide instructions for collecting and handling biochemical sampling and processing related to biometric data gathering as well as capturing biometric data using digital data capturing devices. The user interface module and display are integrated with analysis and communications portions of the biometric biochemical analysis system to provide a portable system for multi-portion data collecting, storage, verification, and analysis.
G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
G10L 17/00 - Speaker identification or verification techniques
G06F 19/10 - Bioinformatics, i.e. methods or systems for genetic or protein-related data processing in computational molecular biology (in silico methods of screening virtual chemical libraries C40B 30/02;in silico or mathematical methods of creating virtual chemical libraries C40B 50/02)
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
A command center includes at least one network communications interface configured for two-way communications with a plurality of sites remote from the command center and at least one display screen and user interface. Each of the plurality of sites includes at least one forensic field test device configured to identify individuals using DNA samples from the individuals. The display screen and user interface are configured to depict aspects of forensic field test devices of the plurality of sites, wherein the aspects include a site identifier for each of the forensic field test devices and one or more additional aspects.
A live help system provides an intuitive display of help information on a user's graphical user interface. A request is received from a client device for help, and a live help provider interface is initiated at a live help location. Data is acquired regarding a user's location, including data on external devices in the user's location. Indicators are provided to allow the live help provider to point to or otherwise indicate items on the user interface or outside of the user interface. Live help input is captured at the live help provider interface. Instructions are then transmitted to the display of the client device to display live help input, as though the agent were present and interacting with or indicating items on the screen or off the screen.
G06F 3/00 - Input arrangements for transferring data to be processed into a form capable of being handled by the computerOutput arrangements for transferring data from processing unit to output unit, e.g. interface arrangements
Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.
This disclosure provides, among other things, a cartridge comprising: (a) a cartridge body comprising a malleable material and having, disposed on a surface of the body, at least one valve body comprising a valve inlet and a valve outlet, each fluidically connected to a fluidic channel; and (b) a layer comprising a deformable material bonded to a surface of the cartridge body and sealing the at least one valve body at points of attachment, thereby forming at least one valve; wherein the at least one valve body is depressed in the cartridge body relative to the points of attachment and wherein the deformable material covering the at least one valve body retains sufficient elasticity after deformation such that in a ground state the valve is open. Also disclosed is an instrument comprising a cartridge interface and a cartridge as described herein engaged with the cartridge interface, wherein (II) the cartridge interface comprises: (A) at least one mechanical actuator, each mechanical actuator positioned to actuate a valve; and (B) at least one motor operatively coupled to actuate a mechanical actuator toward or away from a valve.
This disclosure provides, among other things, a cartridge comprising: (a) a cartridge body comprising a malleable material and having, disposed on a surface of the body, at least one valve body comprising a valve inlet and a valve outlet, each fluidically connected to a fluidic channel; and (b) a layer comprising a deformable material bonded to a surface of the cartridge body and sealing the at least one valve body at points of attachment, thereby forming at least one valve; wherein the at least one valve body is depressed in the cartridge body relative to the points of attachment and wherein the deformable material covering the at least one valve body retains sufficient elasticity after deformation such that in a ground state the valve is open. Also disclosed is an instrument comprising a cartridge interface and a cartridge as described herein engaged with the cartridge interface.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
F16K 7/00 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage
F16K 7/16 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with flat, dished, or bowl-shaped diaphragm arranged to be deformed against a flat seat the diaphragm being mechanically actuated, e.g. by screw-spindle or cam
F16K 99/00 - Subject matter not provided for in other groups of this subclass
G01N 27/26 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variablesInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by using electrolysis or electrophoresis
Provided herein is a system and method for review of forensic report computer files. The method can involve notifying a plurality of service providers of a job to be performed, accepting a bid from a service provider to perform the job, providing the computer file to the service provider, receiving from the service provider a forensic result such as a reviewed file.
Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.
09 - Scientific and electric apparatus and instruments
Goods & Services
Electronic and mechanical apparatus and instruments, and related computer software for scientific, medical, environmental, veterinary, food, agriculture, supply chain management, and forensic research, analysis, testing and diagnostics, namely, fluid handling devices, apparatus to automate chemical or biochemical reactions, thermal cyclers, capillary electrophoresis devices, detection devices, namely, detection of nucleic acids in biological samples; laboratory instruments for human identification, nucleic acid sequencing, or the detection of pathogens in a biological sample; equipment for automating or integrating biochemical processes or biochemical sample preparation, namely, for processing samples for the preparation or analysis of biomolecules; equipment for nucleic acid preparation, handling or analysis, namely, for processing samples for the preparation or analysis of biomolecules; computer and software for processing data in the fields of scientific, medical, environmental, veterinary, food, agriculture, supply chain management, and forensic research, analysis, testing and diagnostics; microfluidic devices for processing samples for the preparation and analysis of biomolecules; integrated fluidic circuits
53.
Systems and methods for sample preparation, processing and analysis
The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.
The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system. An electrophoresis cartridge adapted to releasably engage with a cartridge interface of a system is provided. The electrophoresis cartridge comprises: an electrophoresis assembly including: (1) an anode sub-assembly comprising an anode, (2) a cathode sub-assembly comprising a cathode; and (3) at least one electrophoresis capillary having a first end and a second end, wherein the cathode and the anode are configured to provide a voltage gradient across the first end and the second end of the at least one electrophoresis capillary.
G01N 27/49 - Systems involving the determination of the current at a single specific value, or small range of values, of applied voltage for producing selective measurement of one or more particular ionic species
Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense. Also disclosed in the present disclosure is a flow through, traveling-wave, bead-beating device which comprises a rotating pole piece, a flow through tube, and a magnetic piece. Rotation of the rotating pole piece may create a magnetic wave down the flow through tube, thereby producing sufficient acceleration of beads through the tube to disrupt or lyse target analytes flowing through the tube.
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
G01N 15/06 - Investigating concentration of particle suspensions
B01J 19/08 - Processes employing the direct application of electric or wave energy, or particle radiationApparatus therefor
B01J 19/12 - Processes employing the direct application of electric or wave energy, or particle radiationApparatus therefor employing electromagnetic waves
B01D 21/00 - Separation of suspended solid particles from liquids by sedimentation
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
B01F 11/00 - Mixers with shaking, oscillating, or vibrating mechanisms
B01F 13/00 - Other mixers; Mixing plant, including combinations of dissimilar mixers
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.
Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.
This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
The disclosure provides compositions comprising a biological material (e.g., a protein, a nucleic acid or a biological sample, or any combination thereof) in a substantially water-free, non-ionic or ionic organic solvent. To enhance, e.g., the stability and/or the solubility of the biological material in the substantially water-free fluid medium, the composition can comprise one or more substances (e.g., an antioxidant) described in the disclosure, and/or a metal salt. The biological material is soluble and stable, and retains its function and activity, when it is preserved in the substantially water- free fluid medium at ambient temperature or higher for extended periods of time. Therefore, the composition comprising the biological material does not need to be refrigerated or frozen during shipping or storage.
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.
This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
G01N 21/75 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
69.
SAMPLE PREPARATION, PROCESSING AND ANALYSIS SYSTEMS
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
One aspect of the technology relates to a method comprising the steps of: moving a frame movably holding at least one hollow bore pin having a liquid dispensing tip, such that the liquid dispensing tip of the at least one hollow bore pin moves into a respective well of a set of target wells, and the liquid dispensing tip makes contact with a bottom surface of the respective well; continuing to move the frame after the liquid dispensing tip makes contact, such that the at least one hollow bore pin moves to a particular position (e.g., vertical position) within a range of positions (e.g., vertical positions) relative to and permitted by the frame.
This invention provides a method for generating short tandem repeat (STR) profiles on each of a plurality of samples comprising, for each sample: a) isolating DNA from the sample; b) amplifying STR markers in the isolated DNA and c) analyzing the amplification product by electrophoresis.
The present invention provides methods and devices for performing nucleic acid amplification and sequencing on a solid substrate (e.g., a flow cell), including preparation of libraries of amplified DNA fragments for massively parallel (next-generation) sequencing.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Biochemical reagents for use in scientific, medical,
environmental, veterinary, food, agriculture and forensic
research; reagent kits comprised of biochemical reagents for
use in scientific, medical and forensic research;
biochemical reagents for use in scientific apparatus for
chemical or biological analysis; diagnostic reagents for
scientific or research use; reagent kits comprised of
biochemical reagents for use in scientific apparatus for
chemical or biological analysis. Medical diagnostic reagents for the analysis of body fluids;
diagnostic kits consisting primarily of reagents for use in
disease testing. Electronic and mechanical apparatus and instruments, and
related computer software for scientific, medical,
environmental, veterinary, food, agriculture, and forensic
research, analysis, testing and diagnostics, namely, fluid
handling devices, apparatus to automate chemical or
biochemical reactions, thermal cyclers, capillary
electrophoresis devices, detection devices, namely,
detection of nucleic acids in biological samples; laboratory
instruments for human identification, nucleic acid
sequencing, or the detection of pathogens in a biological
sample; equipment for automating or integrating biochemical
processes or biochemical sample preparation, namely, for
processing samples for the preparation or analysis of
biomolecules; equipment for nucleic acid preparation,
handling or analysis, namely, for processing samples for the
preparation or analysis of biomolecules; computer hardware;
computer software for processing data in the fields of
scientific, medical, environmental, veterinary, food,
agriculture, and forensic research, analysis, testing and
diagnostics; microfluidic devices for processing samples for
the preparation and analysis of biomolecules; integrated
fluidic circuits. Diagnostic kits consisting primarily of reagents for use in
biomolecular testing for genetic analysis for example point
of care nucleic acid testing; biochemical reagent cassettes,
namely, for dispensing of reagents for medical and
veterinary diagnostics; biochemical reagent cassettes,
namely, for use in genetic analysis for medical and
veterinary diagnostics; diagnostic reagent cartridges for
medical and veterinary use.
This invention provides a device comprising at least one diaphragm valve actuated by a hydraulic actuation system. The device is comprised in a combination that includes a fluidics layer, an actuation layer and an elastic layer sandwiched between the fluidics layer and the actuation layer, wherein the diaphragm valve comprises: (a) a valve inlet and a valve outlet comprised in the fluidics layer; (b) a valve seat; (c) a diaphragm comprised in the elastic layer, wherein the diaphragm is actuatable to move into contact or out of contact with the valve seat, thereby closing or opening the diaphragm valve; and (d) an actuator comprising: (1 ) a hydraulic conduit comprised at least in part in the actuation layer; (2) a translator; and (3) an incompressible fluid contained within the hydraulic conduit, wherein the incompressible fluid communicates with the translator and with the diaphragm.
The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.
Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.
The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
B01F 3/00 - Mixing, e.g. dispersing, emulsifying, according to the phases to be mixed
B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
G01N 1/00 - SamplingPreparing specimens for investigation
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Biochemical reagents for use in scientific, medical, environmental, veterinary, food, agriculture and forensic research; Reagent kits comprised of biochemical reagents for use in scientific, medical and forensic research; Biochemical reagents for use in scientific apparatus for chemical or biological analysis; Reagent kits comprised of biochemical reagents for use in scientific apparatus for chemical or biological analysis Electronic and mechanical apparatus and instruments, and related computer software for scientific, medical, environmental, veterinary, food, agriculture, and forensic research, analysis, testing and diagnostics, namely, fluid handling devices, apparatus to automate chemical or biochemical reactions, thermal cyclers, capillary electrophoresis devices, detection devices, namely, detection of nucleic acids in biological samples; laboratory instruments for human identification, nucleic acid sequencing, or the detection of pathogens in a biological sample; equipment for automating or integrating biochemical processes or biochemical sample preparation, namely, for processing samples for the preparation or analysis of biomolecules; equipment for nucleic acid preparation, handling or analysis, namely, for processing samples for the preparation or analysis of biomolecules; computer hardware; computer software for processing data in the fields of scientific, medical, environmental, veterinary, food, agriculture, and forensic research, analysis, testing and diagnostics; microfluidic devices for processing samples for the preparation and analysis of biomolecules; integrated fluidic circuits
This invention provides a capillary electrophoresis device in which capillaries are thermally regulated on a thermally responsive electrical path attached to an electrically insulating circuit board. This invention also provides an optical scanner useful for scanning an array of capillaries. A laser, optical detector and optical selector are in an arrangement that allows the optical detector to selectively detect an optical signal from any one or more of the plurality of electrophoresis capillaries.
A capillary electrophoresis device includes capillaries are thermally regulated on a thermally responsive electrical path attached to an electrically insulating circuit board. An optical scanner scans an array of capillaries. A laser, optical detector and optical selector are in an arrangement that allows the optical detector to selectively detect an optical signal from anyone or more of the plurality of electrophoresis capillaries.
In one aspect, this invention provides an article fabricated in one piece comprising at least one aperture through the piece, wherein the aperture defines a non-micro fiuidic volume, and a micro fiuidic channel formed in a surface of the piece onto which the aperture opens, wherein the channel is in fiuidic communication with the aperture, wherein the aperture and the microfluidic channel define a fiuidic circuit.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Biochemical reagents for use in scientific, medical,
environmental, veterinary, food, agriculture, and forensic
research, analysis, testing and diagnostics; reagent kits
comprised of biochemical reagents for use in scientific,
medical, and forensic research, analysis, and testing. Electronic and mechanical apparatus and instruments for
scientific, medical, environmental, veterinary, food,
agriculture, and forensic research, analysis, testing and
diagnostics; laboratory instruments for human
identification, nucleic acid sequencing, or the detection of
pathogens in a biological sample; equipment for automating
or integrating biochemical processes or biochemical sample
preparation, namely, for processing samples for the
preparation or analysis of biomolecules; equipment for
nucleic acid preparation, handling or analysis, namely, for
processing samples for the preparation or analysis of
biomolecules; computer hardware; computer software for
scientific, medical, environmental, veterinary, food,
agriculture, and forensic research, analysis, testing and
diagnostics; microfluidic devices for processing samples for
the preparation and analysis of biomolecules; fluidic
circuits; biochemical reagent cassettes, namely, for
dispensing of reagents for scientific, medical,
environmental, veterinary, food, agriculture, and forensic
research, analysis, testing and diagnostics; biochemical
reagent cassettes, namely, for use in genetic analysis for
scientific, medical, environmental, veterinary, food,
agriculture, and forensic use; diagnostic reagent cartridges
for scientific, medical, environmental, veterinary, food,
agriculture, and forensic use.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Biochemical reagents for use in scientific, medical,
environmental, veterinary, food, agriculture, and forensic
research, analysis, testing and diagnostics; reagent kits
comprised of biochemical reagents for use in scientific,
medical, and forensic research, analysis, and testing. Electronic and mechanical apparatus and instruments for
scientific, medical, environmental, veterinary, food,
agriculture, and forensic research, analysis, testing and
diagnostics; laboratory instruments for human
identification, nucleic acid sequencing, or the detection of
pathogens in a biological sample; equipment for automating
or integrating biochemical processes or biochemical sample
preparation, namely, for processing samples for the
preparation or analysis of biomolecules; equipment for
nucleic acid preparation, handling or analysis, namely, for
processing samples for the preparation or analysis of
biomolecules; computer hardware; computer software for
scientific, medical, environmental, veterinary, food,
agriculture, and forensic research, analysis, testing and
diagnostics; microfluidic devices for processing samples for
the preparation and analysis of biomolecules; fluidic
circuits; biochemical reagent cassettes, namely, for
dispensing of reagents for scientific, medical,
environmental, veterinary, food, agriculture, and forensic
research, analysis, testing and diagnostics; biochemical
reagent cassettes, namely, for use in genetic analysis for
scientific, medical, environmental, veterinary, food,
agriculture, and forensic use; diagnostic reagent cartridges
for scientific, medical, environmental, veterinary, food,
agriculture, and forensic use.
This invention provides composite plastic articles and methods of making them. The articles can be fluidic or microfluidic devices having fluidic conduits and, optionally, pneumatic conduits that regulate flow in the fluidic conduits. The articles comprise a first substrate coated with a layer of a material that comprises, or onto which have been introduced, reactive groups. For example, the substrate can be a plastic coated with an oxide or a siloxane onto which hydroxyl groups have been introduced. These articles are covalently bonded with other articles comprising reactive groups on their surfaces, for example, polysiloxanes treated to have silanol groups. Certain articles have specified locations on their surfaces that are not bonded to the other piece. For example, the coating can be removed from these locations before bonding. Such locations can be useful as functional elements of various devices, such as valve seats in valves of microfluidic devices.
B23B 7/00 - Automatic or semi-automatic turning-machines with a single working-spindle, e.g. controlled by camsEquipment thereforFeatures common to automatic and semi-automatic turning-machines with one or more working-spindles
This invention provides composite plastic articles and methods of making them. The articles can be fluidic or microfluidic devices having fluidic conduits and, optionally, pneumatic conduits that regulate flow in the fluidic conduits. The articles comprise a first substrate coated with a layer of a material that comprises, or onto which have been introduced, reactive groups. For example, the substrate can be a plastic coated with an oxide or a siloxane onto which hydroxyl groups have been introduced. These articles are covalently bonded with other articles comprising reactive groups on their surfaces, for example, polysiloxanes treated to have silanol groups. Certain articles have specified locations on their surfaces that are not bonded to the other piece. For example, the coating can be removed from these locations before bonding. Such locations can be useful as functional elements of various devices, such as valve seats in valves of microfluidic devices.
F16K 11/20 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit operated by separate actuating members
Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C12M 1/00 - Apparatus for enzymology or microbiology
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
B01D 59/42 - Separation by electrochemical methods by electromigrationSeparation by electrochemical methods by electrophoresis
The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The design of the cartridges, microchips, and pneumatic manifolds can allow for the use of magnetic forces to capture magnetic beads in a chamber formed between the microchip and the cartridge or a chamber within the microchip. The devices of the invention can be used for mRNA amplification and purification.
This invention provides fluidic devices, in particular microfluidic devices, with diaphragm valves having low failure rates Low failure rates are achieved by inhibiting sticking of the diaphragm to Junctional surfaces such as valve seats, valve chamber and fluidic channels and conduits One way to implement this is to provide exposed surfaces facing the diaphragm, particularly valve seats, with a low energy material, such as a noble metal, a perfluoπnated polymer, a self-assembled monolayer, hard diamond, diamond-like carbon or a metal oxide In other embodiments, the valves are provided with ridges and the diaphragm is adhered to the fluidic or actuation layer with an adhesive material.
The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post- reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3, and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.
This invention provides fluidic devices, in particular microfluidic devices, with diaphragm valves having low failure rates. Low failure rates are achieved by inhibiting sticking of the diaphragm to functional surfaces such as valve seats, valve chamber and fluidic channels and conduits. One way to implement this is to provide exposed surfaces facing the diaphragm, particularly valve seats, with a low energy material, such as a noble metal, a perfluorinated polymer, a self-assembled monolayer, hard diamond, diamond-like carbon or a metal oxide. In other embodiments, the valves are provided with ridges and the diaphragm is adhered to the fluidic or actuation layer with an adhesive material.
This invention provides microfluidic devices that comprise a fluidics layer having microfluidic channels and one or more regulating layers that regulate the movement of fluid in the channels. The microfluidic devices can be used to mix one or more fluids. At least a portion of the fluidics layer can be isolated from the regulating layer, for example in the form of a shelf. Such isolated portions can be used as areas in which the temperature of liquids is controlled. Also provided are instruments including thermal control devices into which the microfluidic device is engaged so that the thermal control device controls temperature in the isolated portion, and a movable magnetic assembly including magnets with shields so that a focused magnetic field can be applied to or withdrawn from the isolated portion or any other portion of the microfluidic device. Also provided are methods of mixing fluids. The methods include stacking a plurality of alternating boluses of different liquids in a microfluidic channel, and moving the stacked boluses through the channel. In another method, the boluses are moved into a diaphragm valve having a volume able to accommodate several boluses, and then pumping the liquids out of the valve.
This invention provides microfluidic devices that comprise a fluidics layer having microfluidic channels and one or more regulating layers that regulate the movement of fluid in the channels. The microfluidic devices can be used to mix one or more fluids. At least a portion of the fluidics layer can be isolated from the regulating layer, for example in the form of a shelf. Such isolated portions can be used as areas in which the temperature of liquids is controlled. Also provided are instruments including thermal control devices into which the microfluidic device is engaged so that the thermal control device controls temperature in the isolated portion, and a movable magnetic assembly including magnets with shields so that a focused magnetic field can be applied to or withdrawn from the isolated portion or any other portion of the microfluidic device. Also provided are methods of mixing fluids. The methods include stacking a plurality of alternating boluses of different liquids in a microfluidic channel, and moving the stacked boluses through the channel. In another method, the boluses are moved into a diaphragm valve having a volume able to accommodate several boluses, and then pumping the liquids out of the valve.
Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
The invention herein provides improved sample injection systems and related methods to create microfluidic devices with symmetrical channel configurations that can produce relatively large sample volumes. An embodiment of the invention provides microfluidic structures with different geometries that are symmetrical from the perspective of a sample load channel and a sample waste channel, which essentially eliminates issues of time offset and other problems commonly associated with twin-T sample formation techniques. A split-injection approach and related methods of sample plug formation are therefore provided.
G01N 27/26 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variablesInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by using electrolysis or electrophoresis
