A method for breeding an improved variety of a forage sorghum-Bacillus megaterium symbiont, and a CAPS marker for rapidly identifying Bacillus megaterium BM18-2. The method includes: when a stigma of a female parent is exerted for 2-3 d, spraying a Bacillus megaterium solution to the stigma, then pollinating same with pollens from anthers of a male parent after the anthers are exerted for 2-5 d, and harvesting mature seeds. The CAPS marker mainly includes a primer pair designed for an SNP site and endonuclease BspT104I, and upstream and downstream primers are respectively SEQ ID Nos: 1-2. The first method for breeding improved variety of forage sorghum containing endophytic Bacillus megaterium. Produced seeds can significantly increase biomass, crude protein, soluble sugar content and roughage grading index (GI) of plants grown in soil with a salt content ≤8‰; and the CAPS marker can realize rapid qualitative identification of Bacillus megaterium BM18-2.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
2.
METHOD FOR CULTIVATING ALCOHOL-BREWING SORGHUM ON BASIS OF BIODEGRADABLE MULCHING FILM, AND USE THEREOF
A method for cultivating alcohol-brewing sorghum on the basis of a biodegradable mulching film includes five steps such as farmland preparation, film mulching, sowing operation, field management and harvesting. Compared with a traditional alcohol-brewing sorghum planting method, the method of the present invention can effectively bring forward the planting time of alcohol-brewing sorghum, reduce the sowing amount of alcohol-brewing sorghum, assist in increasing the germination rate of alcohol-brewing sorghum, and promote the growth and development of alcohol-brewing sorghum; moreover, the method of the present invention effectively reduces the labor intensity and cost of a weeding operation in field management.
A01B 79/02 - Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
A01G 13/02 - Protective coverings for plants; Devices for laying-out coverings
3.
Molecular marker significantly associated with vitamin E content in soybeans, kompetitive allele specific polymerase chain reaction primers combination and application thereof
A molecular marker significantly associated with vitamin E content in soybeans, a Kompetitive Allele-Specific polymerase chain reaction (KASP) primers combination and an application thereof are provided in the present disclosure, belonging to the field of molecular genetics and breeding. A nucleotide sequence of the molecular marker is shown in SEQ ID NO. 1, and there is an A/T mutation at the 21st base. The KASP primer set for detecting the molecular marker includes an upstream primer F1 with a nucleotide sequence as shown in SEQ ID NO. 2, an upstream primer F2 with a nucleotide sequence as shown in SEQ ID NO. 3 and a downstream primer r with a nucleotide sequence as shown in SEQ ID NO. 4. The KASP primers combination developed by the present disclosure accurately distinguish soybeans with high and low vitamin E content.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
GUOTAI (TAIZHOU) CENTER OF TECHNOLOGY INNOVATION FOR VETERINARY BIOLOGICALS, TAIZHOU (China)
Inventor
Feng, Zhixin
Yu, Yanfei
Wang, Jia
Wei, Yanna
Shao, Guoqing
Wu, Yuzi
Liu, Maojun
Xiong, Qiyan
Wang, Li
Liu, Beibei
Abstract
A composition and a kit for detecting mycoplasma are provided. The composition for detecting mycoplasma is an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5′ terminus and a quencher BHQ1 linked at a 3′ terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of mycoplasma.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
5.
MULTIPLE KASP MARKER PRIMER SET FOR WHEAT PLANT HEIGHT MAJOR GENES AND USE THEREOF
A multiplex KASP marker primer set for a set of wheat plant height major genes, consisting of a pre-primer having a nucleotide sequence as shown in SEQ ID NO. 10, a post-primer having a nucleotide sequence as shown in SEQ ID NO. 11, and a universal primer having a nucleotide sequence as shown in SEQ ID NO. 9. The multiplex KASP marker primer set achieves the simultaneous detection of the Rht-B1 and Rht-D1 genes.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
6.
USE OF ANTIBACTERIAL CATALYTIC METAL AND MAGNETIC ACID LANTHANIDE OXIDE COMPOSITE MATERIAL IN ELIMINATION OF PHOSPHORUS AND RESISTANCE GENE POLLUTION IN TAIL WATER
Provided in the present invention is the use of an antibacterial catalytic metal and magnetic acid lanthanide oxide composite material in the elimination of phosphorus and resistance gene pollution in tail water, wherein the preparation process for the composite material is as follows: S01, using a dipping-calcining method, mixing a lanthanide element ion solution, a magnetic element ion solution and a carrier, and uniformly stirring, centrifuging and calcining same to obtain a calcined product, i.e., a magnetic acid lanthanide oxide-carrier; S02, using a heating precipitation method, adding the calcined product in S01 to an antibacterial catalytic metal element precursor solution, adding an ammonia water solution thereto in a dropwise manner, and continuously stirring and heating same in a water bath to obtain a mixed system of the composite material; and S03, using a mixed calcining method, calcining the mixed system obtained in S02 to obtain the composite material, i.e., a magnetic acid lanthanide oxide and antibacterial catalytic oxide heterojunction-carrier. The composite material obtained by using the preparation method can be used in the elimination of phosphorus and resistance genes in an agricultural water environment.
GUOTAI (TAIZHOU) CENTER OF TECHNOLOGY INNOVATION FOR VETERINARY BIOLOGICALS, TAIZHOU (China)
Inventor
Xie, Xing
Feng, Zhixin
Song, Daesub
Chen, Rong
Hao, Fei
Xu, Lulu
Yeom, Minjoo
Lim, Jongwoo
Zhang, Lei
Gan, Yuan
Zhao, Long
Li, Wenliang
Xiong, Qiyan
Liu, Yongjie
Liu, Beibei
Yu, Yanfei
Bai, Yun
Shao, Guoqing
Abstract
Provided is a viral vector delivery system for the porcine respiratory tract and digestive tract. The viral vector delivery system comprises a backbone plasmid obtained by inserting full-length cDNA of porcine enterovirus type B into a pUC57 plasmid, and a helper plasmid obtained by inserting a green fluorescent protein-encoding gene into plasmid pCAG-T7-polymerase. The virus titer of the viral vector delivery system can reach 107.755050 per mL, and porcine respiratory tract and digestive tract pathogenic epitopes are inserted into the backbone plasmid, which has a good immune effect and can be used for constructing candidate strains of porcine respiratory tract and digestive tract vaccines.
GUOTAI (TAIZHOU) CENTER OF TECHNOLOGY INNOVATION FOR VETERINARY BIOLOGICALS, TAIZHOU (China)
Inventor
Feng, Zhixin
Yu, Yanfei
Wang, Jia
Wei, Yanna
Shao, Guoqing
Wu, Yuzi
Liu, Maojun
Xiong, Qiyan
Wang, Li
Liu, Beibei
Abstract
The present invention belongs to the technical field of biology. Provided are a composition and kit for detecting mycoplasma. The composition for detecting mycoplasma is characterized in that the composition is an aqueous solution containing primer M-F, primer M-R and probe M-P; the sequence of M-F is as shown in SEQ ID NO: 1; the sequence of M-R is as shown in SEQ ID NO: 2; and the nucleotide sequence of probe M-P is as shown in SEQ ID NO: 3, the 5' end thereof is linked to fluorescent group FAM, and the 3' end thereof is linked to quenching group BHQ1. The composition is used for detecting mycoplasma, has high sensitivity, high specificity, and a wide range of detection.
MMMs meeting required factors for keeping in the database, and using a random forest algorithm, filling missing values in the original data set, so as to obtain a complete database; dividing the complete database into a modeling set and a verification set, and establishing an initial RF-Meta model; training the initial RF-Meta model and optimizing hyper-parameters, so as to obtain an optimized RF-Meta model; importing initial experimental physicochemical data of the degradation of a target biodegradable mulch film into the optimized RF-Meta model, so as to obtain a prediction curve of the full degradation period. The model provided in the invention can achieve, for the first time, specific degradation degree numerical prediction for the degradation process of BDM, and has wide applicability.
The present invention provides a preparation method for a modified cathode of an MEC-AD system and application of the system with high efficiency and low energy consumption. A NiCo-BDC hydrothermal carbon composite MOF material is designed and used for modifying a cathode, which can remove COD more rapidly compared with traditional AD. A wind energy and light energy complementary power generation device is used for energy supply of the MEC-AD system, and energy consumption is further reduced by setting an intermittent power supply mode. The intermittent power supply achieves higher energy recovery efficiency while obtaining higher performance, can effectively reduce energy consumption of the MEC-AD system, is conducive to more convenient configuration of a power generation and energy supply device using clean energy, and has a good application prospect in reducing COD in organic wastewater with low energy consumption and high efficiency.
A combined continuous plasma and radio frequency low-temperature cold sterilization device, comprising: a radio frequency sterilization device, a plasma cold sterilization device, a control device and a transfer device, the transfer device being used for transferring aquatic products to be sterilized, the radio frequency sterilization device and the plasma cold sterilization device being sequentially arranged along a transfer track of said aquatic products, and the control device controlling operation of the radio frequency sterilization device, the plasma cold sterilization device and the transfer device. The radio frequency sterilization device performs low-temperature sterilization on the inside of food and the plasma cold sterilization device performs sterilization on the surface of the food, so that the radio frequency sterilization device and the plasma cold sterilization device achieve complementary sterilization effects, effectively killing microorganisms in food and preventing breeding thereof, thus significantly improving the cold sterilization efficiency.
Provided is a soybean oligosaccharide-related kompetitive allele-specific PCR (KASP) marker and use thereof, belonging to the technical field of molecular breeding. The KASP marker includes one or two of S18_51868868 T/G and S10_38081012 T/C, where the S18_51868868 T/G is a base T or a base G at a 51868868bp position on chromosome 18 of a soybean genome; and the S10_38081012 T/C is a base T or a base C at a 38081012bp position of chromosome 10 of the soybean genome. In the present disclosure, the KASP marker can accurately genotype the traits of raffinose and stachyose contents. Through genotyping, it is found that a soybean germplasm with a genotype GG has a higher raffinose content than that of a soybean germplasm with a genotype TT, and a soybean germplasm with a genotype TT has a higher stachyose content than that of a soybean germplasm with a genotype CC.
An amphiphilic imiquimod-grafted lauryl γ-polyglutamate is prepared by adopting a method comprising the following steps: (1) dispersing γ-polyglutamic acid in an aprotic solvent in an anhydrous atmosphere, then adding a catalyst N,N-dimethylformamide, adding dropwise a chlorinating agent under stirring, and keep reaction of the mixture for 10-40 hours; (2) adding imiquimod, liposoluble alcohol and an acid-binding agent into the solution obtained in the step (1) after reaction, and reacting for 42-54 hours; and (3) after purification, obtaining an amphiphilic imiquimod-grafted lauryl γ-polyglutamate material.
A61K 39/39 - Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
A61K 39/00 - Medicinal preparations containing antigens or antibodies
A61K 47/59 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
Exiguobacterium indicum YAN2 having high-concentration selenite tolerance, the preservation number thereof being GDMCC No. 61594, and a method for fermenting inorganic selenium into nano-selenium by using the strain are provided. The obtained nano-selenium can promote the growth of leaf vegetables, increase the content of chlorophyll, Vc, and total phenol, etc, reduce the content of nitrate, and improve the quality of leaf vegetables. In addition, the present application further provides a nano-selenium liquid fertilizer prepared by fermentation using the strain.
Provided is a multi-layer, intelligent and environment-friendly breeding structure for meat waterfowl, including house bodies, a manure treatment room, staircases and feeders. A bottom end net bed and an upper layer net bed are fixedly connected in the house body, and the upper layer net bed is located above the bottom end net bed. Manure dropping mechanisms are arranged side by side between the bottom end net bed and the upper layer net bed. Conveying mechanisms are arranged respectively on both sides of each of the manure dropping mechanisms. A plurality of conveyor belt conveyors are arranged side by side below the bottom end net bed. A transfer mechanism is arranged at one side of the each of the house bodies, and the transfer mechanism is used for collecting manure on the conveyor belt conveyors and the conveying mechanisms and transporting the manure to the fermentation mechanism.
A01K 31/04 - Dropping-boardsDevices for removing excrement
A01K 39/04 - Combined feeding and drinking appliances
16.
SINGLE-NUCLEOTIDE POLYMORPHISM LOCUS SIGNIFICANTLY RELATED TO YARDLONG BEAN ANTHOCYANIN CONTENT, KOMPETITIVE ALLELE-SPECIFIC POLYMERASE CHAIN REACTION MARKERS AND APPLICATION THEREOF
An SNP marker significantly related with yardlong bean anthocyanin content is provided in the present disclosure. The SNP marker significantly related with yardlong bean anthocyanin content is located at the position of 2,361,292 bp on chromosome 5 of yardlong bean genome v1.2, with a base A to G substitution, and the nucleotide sequence thereof is shown in SEQ ID NO. 1. KASP marker based on the SNP marker significantly related with the yardlong bean anthocyanin content and application thereof are also provided in the present disclosure. The KASP marker and the SNP marker significantly related with yardlong bean anthocyanin content are applied for molecular marker-assisted selection breeding of yardlong bean anthocyanin traits.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
17.
GREEN CLEANING AND BACTERIA REDUCTION METHOD FOR SHRIMPS USING ULTRASOUND-COUPLED JET PLASMA ACTIVATED WATER
A green cleaning and bacteria reduction method for shrimps using ultrasound-coupled jet plasma activated water. The method comprises the following steps: selecting fresh shrimps as a raw material; soaking the shrimps in saline water, such that the shrimps discharge waste from gills and intestinal glands; putting the shrimps, which have discharged waste, into a cleaning pool, and performing cleaning and bacteria reduction by using ultrasound-coupled jet plasma activated water; and draining the shrimps after cleaning, packing the shrimps in a packaging box, performing modified atmosphere packaging by using nitrogen and oxygen, and then refrigerating shrimp products at a temperature of 0-4°C for storage. By means of a cleaning method using ultrasound-coupled jet plasma activated water, waste can be effectively removed from surfaces and crevices of shrimps, and it is not required to use a chemical cleaning agent, such that the quality of the shrimps can be effectively improved, and the shelf life of products is prolonged, making the products have relatively strong market competitiveness.
A22C 29/02 - Processing shrimps, lobsters or the like
18.
BEAN COMMON MOSAIC VIRUS ISOLATE, METHOD FOR CONSTRUCTING FULL-LENGTH INFECTIOUS CDNA CLONE OF BEAN COMMON MOSAIC VIRUS ISOLATE AND USE OF BEAN COMMON MOSAIC VIRUS ISOLATE
Provided are bean common mosaic virus isolate BCMV-RD03, the construction of a full-length infectious cDNA clone of the bean common mosaic virus isolate, and the use of the bean common mosaic virus isolate. The bean common mosaic virus isolate BCMV-RD03 is deposited in the China Center for Type Culture Collection on November 23, 2022, with the deposit number of CCTCC NO: V202288. Provided is a bean common mosaic virus that can infect tobacco, breaking the species boundary in the aspect of the research on the resistance to the bean common mosaic virus and expanding the relevant research to tobacco. The construction of the full-length infectious cDNA clone of the bean common mosaic virus isolate is also performed and the use of the bean common mosaic virus isolate is further provided. A bean common mosaic virus strain that can infect tobacco is selected to construct an infectious cloning vector, and by means of transforming agrobacteria and injecting into tobacco, the systemic infection is successfully formed on the tobacco.
C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
C12N 15/40 - Proteins from RNA viruses, e.g. flaviviruses
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 6/82 - Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
Disclosed is an ultrafine straw/calcium carbonate powder-based method for preparing a mulch film, comprising the following steps: (1) mixing coarse straw powder with ultrafine calcium carbonate powder, crushing and sieving the mixture to obtain ultrafine straw/calcium carbonate powder, wherein the ultrafine straw/calcium carbonate powder is 500-2000 mesh; (2) mixing PBAT, PLA, a compatibilizer, epoxidized soybean oil, and a silane coupling agent with the ultrafine straw/calcium carbonate powder, and producing the mixture into composite particles by a twin-screw extrusion granulator; and (3) performing film blowing on the composite particles by means of a film blowing machine to produce a mulch film. In the method of the present invention, the yield of 1000 mesh straw powder is above 30%; the addition of calcium powder can greatly improve a straw crushing effect, and can also effectively avoid electrostatic adsorption or agglomeration of the calcium powder and of the straw powder; and the calcium powder can act as a mulch film anti-blocking agent and does not need to be separated from the straw powder. Also disclosed is a mulch film having good tensile strength and elongation at break prepared by the method.
The present invention relates to the field of biotechnology, and provides a cell wall synthesis-related protein UGMA, a gene encoding same, and a use thereof. The use of the cell wall synthesis-related protein UGMA or the gene encoding the protein comprises any one of: a use in reducing the virulence of a plant fungal pathogen; a use in inhibiting the growth and development of a plant fungal pathogen; a use in screening and/or preparation of a UGMA protein inhibitor; and a use in screening and/or preparation of a chemical for a plant fungal disease, wherein the amino acid sequence of UGMA is shown in SEQ ID NO: 1; the nucleotide sequence of the gene is shown in SEQ ID NO: 2. By knocking out a ugmA gene in pathogenic bacteria, a ugmA gene deletion mutant is obtained, and it is found that the growth and virulence thereof are severely inhibited, and the cell wall thereof is abnormally thickened. Targeted compounds are also obtained by means of virtual screening, thereby providing a new way for research and development of green agrochemicals.
The present invention belongs to the cross technique field of plant-microorganism, and specifically relates to a method for breeding an improved variety of a forage sorghum-bacillus megaterium symbiont. The method comprises the following steps: when a stigma of a female parent is exerted for 2-3 d, spraying a bacillus megaterium solution to the stigma, then pollinating same with pollens from anthers of a male parent after the anthers are exerted for 2-5 d, and harvesting mature seeds. Further disclosed in the present invention is a CAPS marker for rapidly identifying bacillus megaterium BM18-2. The CAPS marker mainly comprises a primer pair designed for an SNP site and endonuclease BspT104I, and an upstream primer and a downstream primer of the primer pair are respectively shown as SEQ ID Nos: 1-2. Provided in the present invention is the first method for breeding the improved variety of the forage sorghum containing endophytic bacillus megaterium. Produced seeds can significantly increase the biomass, crude protein, soluble sugar content and roughage grading index (GI) of plants grown in soil with a salt content ≤8‰; and the CAPS marker for identifying bacillus megaterium BM18-2 in the present invention can realize rapid qualitative identification of bacillus megaterium BM18-2.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
22.
BROCCOLI PRESERVATION METHOD FOR SIMPLE COLD-CHAIN CIRCULATION
A broccoli preservation method for simple cold-chain circulation, comprising the steps of: electrolyzing a sodium chloride solution by using a slightly acidic hypochlorous acid water generator to prepare slightly acidic electrolyzed water with a pH value of 5.0 to 6.5 and effective chlorine concentration of 300 to 400 mg/L; turning the prepared slightly acidic electrolyzed water into crushed ice by means of an ice maker; placing the broccoli pre-cooled to a final temperature of 0±1℃ into a foam box according to a simple cold chain circulation method commonly used in the production place of the broccoli, covering a layer of non-woven fabric, putting the prepared iced slightly acidic electrolyzed water above the non-woven fabric, the covering ice being one third of the gram weight of the broccoli in the box; and finally, using an adhesive tape to seal the foam box for circulation. The method has a bacteriostatic effect on harvested broccoli in the simple cold-chain circulation process, delays the yellowing and aging of the broccoli, and effectively extends the shelf life of the harvested broccoli.
Soyasaponin-related Kompetitive Allele Specific polymerase chain reaction (KASP) markers and an application thereof are provided in the present application, belonging to the technical field of molecular breeding. The KASP markers include one or two of S07_43139773G/T, and S07_43139033A/G; the S07_43139773G/T comprises a base of G/T at a position of 43139773 bp on chromosome 7 of a soybean genome, the S07_43139033A/G comprises a base of A/G at a position of 43139033 bp on the chromosome 7 of the soybean genome. The KASP markers are applied to improve soyasaponin germplasm breeding.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
The present invention provides a soybean oligosaccharide-related KASP marker and use thereof and belongs to the technical field of molecular breeding. The KASP marker comprises one or two of S18_51868868T/G and S10_38081012T/C. The S18_51868868T/G represents that a base at the position 51868868bp of a No.18 chromosome of a soybean genome is T/G. The S10_38081012T/C represents that a base at position 38081012bp of a No. 10 chromosome of the soybean genome is T/C. The KASP marker of the present invention can be used for accurate genotyping of content traits of raffinose and stachyose. By means of genotyping, it is found that the raffinose content of a soybean germplasm with a genotype of GG is higher than that of a soybean germplasm with a genotype of TT, and the stachyose content of the soybean germplasm with the genotype of TT is higher than that of a soybean germplasm with a genotype of CC. Therefore, the KASP marker of the present invention can be used for screening and breeding of soybean germplasm with high oligosaccharide content, and using the KASP marker of the present invention in selection for early breeding reduces the workload in soybean breeding and has important significance in accelerating the soybean breeding process.
The present invention provides a soybean saponin-related KASP marker and use thereof, and belongs to the technical field of molecular breeding. The KASP marker comprises one or two of S07_43139773G/T and SO7_43139033A/G. The S07_43139773G/T represents that a base at position 43139773bp of a No. 7 chromosome of a soybean genome is G/T. The S07_43139033A/G represents that a base at position #43139033bp of the No. 7 chromosome of the soybean genome is A/G. The KASP marker of the present invention can be used for screening and breeding of soybeans with high soybean saponin content germplasm, and PCR amplification can be carried out only by extracting plant leaf genome DNA, which contributes to deep research of soybean saponin-related genes, screening of soybeans with high soybean saponin content, and soybean molecular marker-assisted breeding.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Provided are a plant immunity activation protein PmSCR1 and a use thereof, relating to the technical field of biological crop prevention and treatment. A novel plant immunity activation protein is identified from Pythium myriotylum and is named PmSCR1. The plant immunity activation protein has relatively strong activity, can induce defense reaction of plants, and can be applied to prevention and treatment of crop diseases, in particular, prevention and treatment of plant diseases caused by Pythium myriotylum, Phytophthora sojae and Sclerotinia sclerotiorum.
Provided are a PCR primer for sex identification of young pigeons, use thereof, and a method for sex identification of young pigeons. The primer is designed for a specific EE0.6 sequence fragment on the W chromosome of female pigeons. During detection, Biotin and FAM are used for modifying the primer. Firstly, an efficient nucleic acid lysis solution is used for rapidly extracting DNA in feather pulp, thereby simplifying the DNA-obtaining process; then specific PCR amplification is carried out by using the modified primer, and an amplified PCR product is used for rapidly identifying female individuals by means of the presence or absence of a band on a colloidal gold test strip. The method has the advantages of high specificity, high accuracy rate, simple and convenient operation, rapid and accurate identification result, small damage to animals, and the like, thereby reducing the time and economic cost of pigeon sex identification, and having wide market application prospects. The detection result of the method is consistent with the result of the colloidal gold test strip, and the accuracy rate is 100%.
A preparation method for an orchard base fertilizer and an application method therefor, the preparation method comprising: 1) mixing a microbial powder and a wall material solution to prepare microcapsules, and then adding organic matter and a binder for granulation to obtain a spherical microbial granular organic fertilizer; 2) evenly mixing a cellulose-based polyol and a curing agent and spraying same on the surface of a core fertilizer, and then adding paraffin to obtain a controlled-release fertilizer; and 3) evenly mixing the microbial granular organic fertilizer and the controlled-release fertilizer to obtain an orchard base fertilizer. The base fertilizer can be used as a base fertilizer for fruit trees by using a furrow application or hole application method. The release of nutrients meets fertilizer requirements for fruit trees, while also increasing the nutrient supply intensity of orchard soil.
C05G 3/00 - Mixtures of one or more fertilisers with additives not having a specifically fertilising activity
C05G 3/40 - Mixtures of one or more fertilisers with additives not having a specifically fertilising activity for affecting fertiliser dosage or release rateMixtures of one or more fertilisers with additives not having a specifically fertilising activity for affecting solubility
C05G 3/90 - Mixtures of one or more fertilisers with additives not having a specifically fertilising activity for affecting the nitrification of ammonium compounds or urea in the soil
A preparation method of a crop straw-based seawater desalinator and a product thereof, and the preparation process is as follows: (1) crushing crop straws, and screening the crushed crop straws through a mesh sieve to obtain straw powder; (2) calcining the straw powder at a high temperature under nitrogen protection to obtain biochar, and then putting the biochar into a ball mill for ball milling to obtain ball-milled biochar; (3) adding the straw powder into a sodium hydroxide solution for reaction, and centrifuging a reactant to obtain a viscous product; and (4) stirring the viscous product uniformly with the ball-milled biochar to obtain a mixture, pouring the mixture into a mold, and freezing and drying the mixture to obtain a seawater desalinator. The evaporator features high evaporation rate and the photothermal conversion efficiency, and the ion concentration of the water body after evaporation is greatly reduced.
Disclosed in the present invention is a multi-tier, intelligent, and environment-friendly meat-type waterfowl breeding structure, comprising: house bodies, excrement treatment rooms, staircases, and feeders. A bottom end net bed and an upper tier net bed are fixedly connected in each house body; the upper tier net bed is located above the bottom end net bed; a plurality of excrement discharging mechanisms are arranged side by side between the bottom end net bed and the upper tier net bed; conveying mechanisms are arranged on either side of each excrement discharging mechanism; a plurality of belt conveyors are arranged side by side below the bottom end net bed; a transfer mechanism is disposed on one side of the house body and is used for collecting excrement on the belt conveyors and the conveying mechanisms and transferring the excrement to a fermentation mechanism; feeding mechanisms are provided above the bottom end net bed and above the upper tier net bed; the feeders are communicated with the feeding mechanisms; and an air exhaust mechanism is mounted on the house body. The present invention uses a multi-tier structure, so as to reduce an occupied area and provide conditions similar to those of ground breeding; in addition, the present invention uses intelligent environment control and intelligent feeding, so as to provide better conditions for meat-type waterfowl breeding.
A preparation method for a crop straw-based seawater desalinator and a product thereof. A preparation process is as follows: (1) crushing crop straws, and sieving same with a mesh sieve to obtain straw powder; (2) calcining the straw powder at a high temperature under the protection of nitrogen to obtain biochar, and then ball milling the biochar in a ball mill to obtain ball-milled biochar; (3) adding the straw powder into a sodium hydroxide solution for a reaction, and centrifuging a reactant to acquire a lower-layer viscous product; and (4) uniformly stirring and mixing the viscous product and the ball-milled biochar, pouring same into a mold, and freezing and drying same to obtain a product. The evaporator has the following advantages of a high rate of evaporation and photo-thermal conversion efficiency, as well as a greatly-reduced concentration of ions in an evaporated water body satisfying the drinking water regulations, and the evaporator exhibits a low raw material cost and a simple preparation process while not involving a complicated treatment process, thus having wide application prospects in the field of seawater desalination.
Provided are a nano chitosan/dsGRK complex, a preparation method therefor, and an application thereof. The nano chitosan/dsGRK complex comprises dsGRK and chitosan, and the degree of deacetylation of the chitosan is 75%. An apolygus lucorum nano chitosan/dsGRK complex can effectively improve the stability of the dsGRK. The death rate of the apolygus lucorum in the nano chitosan/dsGRK complex treatment group is significantly improved.
An LED light source for adult Hermetia illucens breeding and an adult Hermetia illucens breeding method. The LED light source for factory breeding of adult Hermetia illucens comprises four wavebands of light, respectively having the following wavelengths and proportions: 360-370 nm of ultraviolet light accounting for 4-10%; 440-460 nm of blue light accounting for 41-50%; 510-520 nm of green light accounting for 32-39%; and 630-650 nm of red light accounting for 8-15%. The LED light source can shorten the adult breeding cycle, thereby greatly improving the spawning production efficiency.
The present invention relates to the field of molecular genetic breeding, and particularly, to an SNP marker closely associated with anthocyanin content in Vigna unguiculata, a KASP molecular marker primer, a kit, and use thereof. The SNP marker provided by the present invention is located at bp position 2,361,292 of chromosome 5 in Vigna unguiculata genome V1.2, and is an A-to-G substitution. The nucleotide sequence of the wild-type gene of anthocyanin in Vigna unguiculata is set forth in SEQ ID NO 1, in which position 479 comprises the A-to-G mutation.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
36.
MULTIPLEX KASP MARKER PRIMER SET FOR WHEAT PLANT HEIGHT MAJOR GENES AND USE THEREOF
A multiplex KASP marker primer set for a set of wheat plant height major genes, consisting of a pre-primer having a nucleotide sequence as shown in SEQ ID NO. 10, a post-primer having a nucleotide sequence as shown in SEQ ID NO. 11, and a universal primer having a nucleotide sequence as shown in SEQ ID NO. 9. The multiplex KASP marker primer set achieves the simultaneous detection of the Rht-B1 and Rht-D1 genes.
22BDC; the preparation of an MOF/polyacrylonitrile mixed nanofiber membrane; and the preparation of a stainless-steel-mesh-coated electrostatically-spun membrane composite electrode. The composite electrode can effectively promote the efficient degradation of COD in an electrically enhanced anaerobic digestion process, and can shorten the time of the anaerobic digestion reaction process and improve the treatment efficiency. In addition, the electrode has good stability and conductivity and relatively low cost, and has good application prospects in the aspect of efficiently removing COD in aquaculture sewage.
D04H 1/4382 - Stretched reticular film fibresComposite fibresMixed fibresUltrafine fibresFibres for artificial leather
D04H 1/728 - Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
Disclosed are a method for preparing a porcine-derived interferon-delta 5 (pIFN-δ5) and an application of the pIFN-δ5, where the method for preparing pIFN-δ5 includes the following steps: step S1, obtaining a DNA fragment containing pIFN-δ5 gene through reverse transcription-polymerase chain reaction (RT-PCR) amplification by using the total RNA of pretreated porcine small intestinal epithelial cells IPEC-J2; step S2, inserting the DNA fragment containing pIFN-δ5 gene into an exogenous expression vector to construct a recombinant expression vector for expressing the pIFN-δ5 gene; and step S3, introducing the recombinant expression vector into a suitable host cell, and driving the host cell to express the pIFN-δ5 gene to obtain the pIFN-δ5. The recombinant pIFN-δ5 protein is used to prepare drugs or preparations for inhibiting infection of porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine delta coronavirus (PDCoV) and porcine rotavirus (PRoV).
Provided are a method for preparing pIFN-δ5 and the use of the pIFN-δ5. The method comprises: step S1, acquiring a DNA fragment containing a pIFN-δ5 gene via RT-PCR amplification by means of pretreated porcine IPEC-J2 intestinal epithelial cell total RNA; step S2, inserting the DNA fragment into an exogenous expression vector, and constructing a recombinant expression vector for expressing the pIFN-δ5 gene; and step S3, introducing the recombinant expression vector into a proper host cell, and driving and expressing the pIFN-δ5 gene in the host cell to obtain the pIFN-δ5. The method may easily obtains a large amount of high-purity recombinant pIFN-δ5 protein, and the recombinant pIFN-δ5 protein can be used for preparing drugs or preparations for inhibiting PEDV, TGEV, PDCoV and/or PRoV infection.
The present invention relates to a method for preparing carbon-reduced and carbon-negative products from agricultural and forestry residues. The method comprises a step of biological breeding transformation, a step of fibrosis transformation, a step of preparation of finished products, etc. By means of the present invention, the productivity and land value of polluted land, such as barren and decertified land and salinized land, can be effectively increased; the improvement of soil quality of polluted land, such as barren and decertified land and salinized land, can be facilitated; the mechanical properties and ductility of composites are further improved while enabling the composites to have good degradability; and compared with traditional fully degradable products, the energy consumption and costs for the production of the composites are greatly reduced, and biomass resources serve as one of main raw materials during a production process, such that the aim of realizing "carbon reduction" during the production and obtaining "carbon-negative" products is achieved.
Disclosed in the present invention is a one-step fertilization and industrial seedling raising method for rice, which method relates to the technical field of rice seedling raising. The method of the present invention specifically comprises adding a slow-release fertilizer specially for rice seedling raising into cultivation soil of a rice seedling raising tray during the process of industrial seedling raising of rice. The slow-release fertilizer specially for rice seedling raising comprises the following raw materials in parts by weight: 40-50 parts of vegetable-oil-based coated urea, 40-50 parts of a vegetable-oil-based coated compound fertilizer and 20-35 parts of clay. In the method of the present invention, the processes of industrial seedling raising and fertilization of rice are integrated, and can serve as one-step application of a base fertilizer. Topdressing is not needed throughout the whole growth period, the two fertilization steps of base fertilizer application and topdressing are omitted, the labor and machinery costs are greatly reduced, the economic benefits are improved, and a grower has more free time.
C05G 3/40 - Mixtures of one or more fertilisers with additives not having a specifically fertilising activity for affecting fertiliser dosage or release rateMixtures of one or more fertilisers with additives not having a specifically fertilising activity for affecting solubility
C05G 5/30 - Layered or coated, e.g. dust-preventing coatings
The present invention relates to a method for cultivating alcohol-brewing sorghum on the basis of a biodegradable mulching film, and the use thereof. The method comprises five steps such as farmland preparation, film mulching, sowing operation, field management and harvesting. Compared with a traditional alcohol-brewing sorghum planting method, the method of the present invention can effectively bring forward the planting time of alcohol-brewing sorghum, reduce the sowing amount of alcohol-brewing sorghum, assist in increasing the germination rate of alcohol-brewing sorghum, and promote the growth and development of alcohol-brewing sorghum; moreover, the method of the present invention effectively reduces the labor intensity and cost of a weeding operation in field management, so as to effectively reduce the root interference and nutrient loss caused by weeds to the growth and development of alcohol-brewing sorghum, further improve the yield of alcohol-brewing sorghum, promote the activity of microorganisms in soil, and meet the physiological requirements for the growth of alcohol-brewing sorghum, while effectively eliminating "white pollution" caused by a traditional film mulching operation and the damage to the soil structure caused by excessive use of chemical fertilizers.
The present invention relates to an accurate large-scale indoor breeding method for Chilo suppressalis using rice seedlings, comprising: cleaning and levigating rice seeds; disinfecting the obtained rice seeds; accelerating germination of the rice seeds to obtain rice sprouts, and then accelerating seedlings to obtain rice seedlings; performing refined larva breeding by using the rice seedlings; forming and collecting pupae; imagoes mating and spawning; and collecting and processing egg masses. According to the present invention, the operation steps of preparation of natural feeds-rice seedlings, larva breeding, pupa formation and collection, mating and spawning of the imagoes, and egg mass collection are accurately quantified, a set of large-scale breeding method is formed, and the breeding time, cost and labor are saved.
The invention discloses a traceability system for pesticide residues, the main points of the technical scheme: the traceability system for pesticide residues, comprising the following steps; step 1, collecting the basic properties of a variety of pesticides commonly used in crops; step 2, collecting the degradation properties of pesticides in many different types of soils; step 3, collecting the rainfall, irrigation conditions and other agricultural operation factors; step 4, predicting the risk of pesticide residues on the surface of the soil after use, and speculating on the transfer pollution of pesticides to crops; step 5, predicting the pesticide residues available to leach into groundwater after pesticide use; step 6, predicting the pesticide residues available to leach into surface water after pesticide use. The traceability system for pesticide residues provides a set of practical pesticide residues risk assessment tools for agricultural producers, environmental protection and legislative agencies, agricultural production management departments.
The present invention relates to method for precise indoor mass rearing of the rice striped stem borer, Chilo suppressalis (Walker) using rice seedlings. The procedure involves cleaning and panning of rice seeds, sterilizing the resultant rice seeds, germinating the seeds to obtain rice sprouts and then germinating seeds to obtain rice seedlings, using rice seedlings for controlled larval rearing, allowing pupation and collecting pupae, allowing mating and egg-laying by adults, and collecting and treating the obtained egg masses. The invention precisely quantifies the operational steps of the preparation of rice seedlings as natural feed, larval rearing, pupation and pupa collection, adult mating, and egg mass collection, forming a set of mass rearing procedures that save rearing time, cost, and labor.
A01K 67/033 - Rearing or breeding invertebrates; New breeds of invertebrates
A23K 10/30 - Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hayAnimal feeding-stuffs from material of fungal origin, e.g. mushrooms
A23K 50/90 - Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
46.
BURDOCK NUTRITIONAL GRAIN MEAL REPLACEMENT POWDER AND PREPARATION METHOD THEREFOR
A burdock nutritional cereal meal replacement powder, comprising the following components in parts by weight: 20-50 parts by weight burdock powder, 10-20 parts by weight soybean powder, 5-15 parts by weight whey protein powder, 1-10 parts by weight sesame, 3-6 parts by weight tremella powder, 6-12 parts by weight Chinese wolfberry powder, 9-15 parts by weight cooked polygonatum powder, 5-15 parts by weight adzuki beans, 1-5 parts by weight oats, 1-5 parts by weight quinoa, 6-12 parts by weight Polygonatum odoratum powder, 0.001-0.005 parts by weight iron, 0-1 parts by weight sodium, 0.0002-0.0005 parts by weight vitamin A, and 0.01-0.08 parts by weight taurine. The burdock powder is burdock root having undergone modification by steam explosion and synergistic fermentation, and burdock nano dietary fiber powder is prepared using nanocrystallization processing. The present invention further relates to a preparation method for the burdock nutrition grain meal replacement powder, and the obtained burdock nutrition cereal meal replacement powder can adjust diversity of intestinal strains and lower blood sugar.
Disclosed in the present invention are a bifunctional nanobody based on DC, and a construction method therefor and the use thereof. The bifunctional nanobody is a target protein, wherein a target protein specific nanobody based on DC is linked to a gene encoding a virus antigen specific nanobody with a particle size of 150 nm or less using a linker element to obtain a target gene fragment, and the obtained target protein is subjected to recombinant expression. According to the method of the present invention, specific nanobodies for different pathogens are screened, and a corresponding porcine DC targeted bifunctional nanobody is constructed and is used for preparing a vaccine for pigs.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C07K 16/08 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses
An amphiphilic imiquimod grafted γ-lauryl polyglutamate and an application thereof, which belong to the field of biomedical materials. The amphiphilic imiquimod grafted γ-lauryl polyglutamate is prepared by using a method comprising the following steps: (1) dispersing γ-polyglutamic acid in an aprotic solvent in an anhydrous atmosphere, adding catalyst, N,N-dimethylformamide, in a stirring state, adding a chlorinating agent dropwise, and reacting for 10-40 hours; (2) adding imiquimod, a fat-soluble alcohol and an acid-binding agent into the solution obtained after the reaction in step (1), and reacting for 42-54 hours; and (3) after purifying, obtaining an amphiphilic imiquimod grafted γ-lauryl polyglutamate material. The amphiphilic imiquimod grafted γ-lauryl polyglutamate and a fluorescein marker thereof not only have good water solubility and biocompatibility, and reduce toxic and side effects, but can also improve the specific immune response of an organism, and is an ideal adjuvant.
A61K 39/39 - Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
A61K 47/59 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
Disclosed in the present application are an Exiguobacterium indicum YAN2 having high-concentration selenite tolerance, the preservation number thereof being GDMCC No. 61594, and a method for fermenting inorganic selenium into nano-selenium by using the strain. The obtained nano-selenium can promote the growth of leaf vegetables, increase the content of chlorophyll, Vc, and total phenol, etc., reduce the content of nitrate, and improve the quality of leaf vegetables. In addition, the present application further provides a nano-selenium liquid fertilizer prepared by fermentation using the strain.
C12P 3/00 - Preparation of elements or inorganic compounds except carbon dioxide
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
A Bacillus altitudinis ST15 antagonizing Xanthomonas oryzae and use thereof, the Bacillus altitudinis ST15 was deposited in China General Microbiological Culture Collection Center on Jun. 28, 2020 and has the deposit number of CGMCC No. 20156. The Bacillus altitudinis has a relatively high antagonistic activity to Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, can promote growth of rice and improve a drought resistance of a rice seedling, and has a relatively strong tolerance to common chemical microbicides for controlling bacterial diseases of rice. A bacterial suspension or a biocontrol inoculant prepared from this strain has a relatively high biocontrol effect on bacterial blight of rice and bacterial leaf streak of rice, can replace or reduce chemical pesticides, improves safety of food and ecological environment, and has relatively high economic and social benefits.
The present invention relates to the field of veterinary vaccines, and provides a vaccine against a Streptococcus suis disease. According to the vaccine against a Streptococcus suis disease, an antigen is a protein having an amino acid sequence as represented by SEQ ID NO: 2. The present invention further provides a method for preparing the vaccine against a Streptococcus suis disease, comprising the following steps: mixing white oil and aluminum stearate to obtain a white oil adjuvant; adding Tween-80 to an aqueous solution of the protein having the amino acid sequence as represented by SEQ ID NO: 2, and mixing well to obtain an antigen solution; and mixing the antigen solution and the white oil adjuvant according to a volume ratio of 0.5-1.5:2, and emulsifying the mixture to obtain the vaccine. The vaccine against a Streptococcus suis disease of the present invention can effectively resist challenges of Streptococcus suis type 2, type 3 and type 31.
An adjustable single power source automatic cable winding and laying apparatus comprises a rack (1), a drive motor (2) being fixedly connected to one side of the rack, a first friction wheel (3) being connected to a shaft of the drive motor, a second friction wheel (4) being in contact connection with the first friction wheel, a winding drum (5) being in transmission connection with the second friction wheel, the winding drum being rotatably connected to the rack, a speed-regulating cable laying mechanism being in transmission connection with the end of the first friction wheel furthest from the drive motor, and the axes of the first friction wheel and the second friction wheel being perpendicular to one another. The present apparatus transmits power to the winding drum by means of friction transmission and, when overloaded, the friction transmission will interrupt power transmission, thereby protecting the cable.
B65H 75/38 - Cores, formers, supports, or holders for coiled, wound, or folded material, e.g. reels, spindles, bobbins, cop tubes, cans specially adapted or mounted for storing and repeatedly paying-out and re-storing lengths of material provided for particular purposes, e.g. anchored hoses, power cables involving the use of a core or former internal to, and supporting, a stored package of material
INSTITUTE OF TOBACCO, ANHUI ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Zhang, Tifu
Qian, Yiliang
Zhao, Han
Ruan, Long
Abstract
A high-efficiency Zea mays L. breeding method based on individual plant evaluation and genome-wide selection (GWS) includes: S1. in a first cropping season, pollinating a Zea mays L. female parent with multiple male parents; S2. in a second cropping season, subjecting hybrid seeds to single-seed sowing, conducting individual plant selection, and evaluating target traits; S3. identifying a parent of a selected cross combination; S4. subjecting a target trait of a cross combination to genome-wide prediction; S5. selecting an excellent cross combination according to a predicted target trait; and S6. subjecting the selected excellent cross combination directly to variety registration or to further evaluation. The high-efficiency Zea mays L. breeding method provided by the present disclosure greatly reduces a quantity of cross combination seeds obtained in the first cropping season and a planting scale of the cross combinations in the second cropping season, and effectively reduces the breeding cost.
Disclosed are a Lactobacillus farciminis SR2 and a use thereof. The Lactobacillus shows good tolerance under acidic conditions, has strong adaptability in high temperature and high salt environments, inhibits the growth of harmful bacteria such as Escherichia coli, Staphylococcus aureus, and salmonella, has high productivity of ferulic acid esterase, and can significantly lower the pH level of rice straw silage feed, increase the content of lactic acid, dry matter, and WSC, reduce the content of ammonia nitrogen, and reduce the content of NDF, ADF and cellulose, and can be used in the field of rice straw fermented feed preparation.
A23K 30/18 - Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
A23K 10/12 - Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
A23K 10/37 - Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hayAnimal feeding-stuffs from material of fungal origin, e.g. mushrooms from waste material
A61K 35/747 - Lactobacilli, e.g. L. acidophilus or L. brevis
A photo-curing three-dimensional printing preview method, comprising: obtaining the relationship of the conversion rate of a photosensitive resin system versus time under different curing conditions by measuring the relationship between the absorbance of the photosensitive resin system under different curing conditions and the time; fitting on experimental curves on the basis of a photo-curing kinetic equation by an iterative solution method, and obtaining a photo-curing kinetic constant of the photosensitive resin system; solving a photo-curing reaction kinetic differential equation set by using a fixed-step Euler method, and obtaining the relationship curves of the spatial distribution of each component content of the photosensitive resin system versus time; and conducting simulation printing, in conjunction with the relationship curves obtaining a space distribution of each component content in each layer of structure in a three-dimensional printing product after printing is completed, and completing a photo-curing three-dimensional printing preview.
B29C 64/386 - Data acquisition or data processing for additive manufacturing
B29C 64/124 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
B33Y 50/00 - Data acquisition or data processing for additive manufacturing
G05B 19/4099 - Surface or curve machining, making 3D objects, e.g. desktop manufacturing
56.
EFFICIENT MAIZE BREEDING METHOD BASED ON SINGLE PLANT EVALUATION AND WHOLE GENOME SELECTION TECHNOLOGY
INSTITUTE OF TOBACCO, ANHUI ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Zhang, Tifu
Qian, Yiliang
Zhao, Han
Ruan, Long
Abstract
Disclosed in the present invention is an efficient maize breeding method based on single plant evaluation and whole genome selection technology. The method comprises the following steps: S1, carrying out multi-male parent pollen pollination on maize female parents in a first planting season; S2, in a second planting season, performing single-seed sowing by means of hybrid seeds, selecting single plants and evaluating target traits thereof; S3, identifying selected hybrid combination parents; S4, performing whole genome prediction on target traits of the hybrid combinations; S5, selecting excellent hybrid combinations according to the predicted target traits; and S6, enabling the selected excellent hybrid combinations to directly enter a variety examination and approval link, or continuing to perform evaluation. According to the breeding method provided by the present invention, the amount of seeds in hybrid combination matching in the first planting season and the planting scale of hybrid combinations in the second planting season are greatly reduced, thereby effectively reducing the breeding cost; unmatched hybrid combinations can be predicted to select excellent combinations, thereby reducing the waste of excellent genetic resources, and improving the selection efficiency in breeding practice.
Provided are a sodium alginate-based hydrogel loaded with lutein, and a preparation method therefor and the use thereof. The sodium alginate-based hydrogel loaded with lutein comprises the following ingredients in parts by weight: 3.12-10.53 parts by weight of lutein, 46.8-47.7 parts by weight of chickpea protein isolate, 120-125 parts by weight of sodium alginate, 8.4-42 parts by weight of Ca2+-EGTA, and 7.12-35.6 parts by weight of D-gluconolactone. Further provided is a method for preparing the sodium alginate-based hydrogel loaded with lutein. The obtained sodium alginate-based hydrogel loaded with lutein has a lutein loading capacity of 760 μg/g and an encapsulation efficiency of 96% or more, and shows good drug loading performance and sustained release performance. Further provided is the use thereof in the preparation of a drug for preventing or treating diseases caused by inflammatory factor levels and intestinal microbial structures.
A23L 33/105 - Plant extracts, their artificial duplicates or their derivatives
A61K 47/00 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient
A61K 47/36 - PolysaccharidesDerivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
JIANGSU FLAG CROP PROTECTION TECHNOLOGY CO., LTD. (China)
Inventor
Zhang, Baolong
Yang, Yuwen
Zhou, Zhenzhen
Liu, Tingli
Guo, Dongshu
Abstract
Disclosed are a mutant HPPD protein and a nucleic acid or gene encoding the protein or a fragment. The HPPD protein or a biologically active fragment thereof retains or enhances the property of catalyzing conversion of a 4-hydroxyphenylpyruvic acid (4-HPPA) to a homogentisic acid, and is significantly less sensitive to an HPPD inhibitor than a wild-type HPPD. The present invention further relates to a method for obtaining a plant comprising expression vectors and host cells and having reduced sensitivity to HPPD inhibitor herbicides. The mutant protein having a single point mutation or multiple point mutations obtained in the present invention can tolerate four HPPD inhibitor herbicides such as mesotrione, topramezone, isoxaflutole, and tembotrione in Escherichia coli. The transgenic rice having a single point mutation or multiple point mutations in the present invention can tolerate four HPPD inhibitor herbicides such as mesotrione, topramezone, isoxaflutole, and tembotrione.
A compression roller-type particle extrusion compact forming system with a cutting function. The system comprises two compression rollers (1) mounted in a machine body and driven by a power transmission system to move and act oppositely and a circular mold or flat mold (2), wherein grooves (3) are evenly provided in cylindrical surfaces of the compression rollers (1), cutting ridges (4) are formed on portions, between adjacent grooves (3), on the cylindrical surfaces of the compression rollers (1), the compression rollers (1) and the circular mold or flat mold (2) form the compression roller-type particle extrusion compact forming system, the cutting ridges (4) on the cylindrical surfaces of the compression rollers (1) and an extrusion acting surface of the circular mold or flat mold (2) can respectively form cutting pairs (5), and the compression rollers (1) and the circular mold or flat mold (2) interact with each other, such that materials are effectively grabbed, cut (broken) and extruded, and the materials can smoothly enter a forming mold hole and finish granulation forming.
B01J 2/22 - Processes or devices for granulating materials, in generalRendering particulate materials free flowing in general, e.g. making them hydrophobic by pressing in moulds or between rollers
60.
MELOIDOGYNE-RELATED MIRNA, REGULATORY GENE THEREOF, PROTEIN THEREOF AND APPLICATION THEREOF
Provided are a meloidogyne-related miRNA, a regulatory gene thereof, a protein thereof, and an application thereof. The miRNA is miRcn1, the gene regulated by miRcn1 comprises CRF9, the protein is encoded by the provided meloidogyne-related miRNA regulatory gene, the application comprises resistance to meloidogyne, and experiments confirm that miRNA miRcn1 and the target gene CRF9 thereof have an important effect in controlling meloidogyne. Overexpression of miRNA miRcn1 and silencing the target gene CRF9 can increase plant resistance towards meloidogyne.
A cultivation method based on an LED light source and used for promoting the blossoming and fruiting of tomatoes. After one week of seedling culturing, field planting and seedling recovery, tomatoes are placed in a lighting environment with an LED light source; the LED light source is a combined light source of white light, red light and blue light; and the luminous flux density of the LED light source is 290-300 µmol·m-2·s-1. By means of treatment with an LED light formulation, the number of blossoming tomatoes is increased, such that the tomatoes blossom 5-7 days early and the fruiting time is 8-11 days early, thereby accelerating the fruit production period and improving the quality of the tomatoes.
Provided are Bacillus altitudinis ST15 with preservation number CGMCC No.20156 for antagonizing Xanthomonas oryzae and use thereof. The ST15 strain has relatively high antagonistic activity against both Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, can promote rice growth and improve the drought resistance of rice seedlings, has strong tolerance to common chemical bactericides for controlling rice bacterial diseases, and can be used for preparing a biocontrol agent.
A method for creating a new germplasm of a male sterile crop by gene editing and an application thereof are provided. In this method, the gene editing is performed on an exon region of a Ty-5 gene, and a deletion of DNA sequence is introduced by using a repair mechanism of plants themselves to double-strand breaks (DSBs), causing a loss of function of Ty-5 gene, thereby obtaining a male-sterile character. The method can be applied without being limited by crop categories. After the gene editing is performed on Ty-5 genes of various crops, new germplasms can be quickly obtained. The new germplasms have the same agronomic characters as the previous materials, and only differ in sexual aspect, which effectively solves the problem of the lack of male sterile materials and unstable fertility in natural resources.
The present disclosure relates to a method for efficiently enriching lutein in broccoli sprouts under γ-aminobutyric acid combined with sodium chloride stress. In the present disclosure, Qingfeng broccoli seeds with plump particles, uniform size and germination ability are selected as raw materials; after removing impurities and being disinfected by a NaClO solution, the seeds are sprayed with distilled water for one day, then sprayed with a mixed aqueous solution of NaCl and γ-aminobutyric acid, to obtain broccoli sprouts.
The disclosure discloses a Chinese cabbage plasma membrane intrinsic aquaporin, having an amino acid sequence as set forth in SEQ ID NO. 2, and the nucleotide sequence of the encoding gene BraPIP2;1 thereof is as set forth in SEQ ID NO. 1. The plasma membrane intrinsic aquaporin has the characteristic of sensitively responding to neonicotinoid insecticides (thiamethoxam, imidacloprid, etc.) in the external environment. At the same time, it has the function of mediating transmembrane transport of the neonicotinoid insecticides, promoting accumulation of the neonicotinoid insecticides in plant roots and leaves, which has important application value in guiding efficient and simple use of pesticides, development of new systemic pesticides, etc.
A temperature-sensitive hydrogel adjuvant for veterinary vaccines, a preparation method and the use thereof, which relate to the field of veterinary vaccine adjuvants. The temperature-sensitive hydrogel adjuvant for veterinary vaccines consists of the following components in percentages by mass: 15-50% of a polymer micelle substance, and 0.1-5% of a non-ionic cellulose ether, with the balance being water. The preparation method of a temperature-sensitive hydrogel adjuvant of the veterinary vaccine comprises the specific steps of: weighing out the components, mixing, and swelling into a uniform system at 2-10°C to obtain the temperature-sensitive hydrogel adjuvant. The temperature-sensitive hydrogel adjuvant has simple and readily available raw materials, is low in price, small in viscosity, has few side effects and is capable of prolonging the immune duration.
A quantum dot microsphere immunochromatography test strip for detection of an African swine fever virus mucous membrane sIgA antibody and a use thereof, relating to the field of veterinary biology diagnostic products. The test strip (1) comprises a bottom panel (2), and a sample pad (3), a nitric acid cellulose membrane (4), and a water absorption pad (7) are provided in succession on the bottom panel (2); a detection line (5) and a quality control line (6) are provided on the nitric acid cellulose membrane (4), the detection line (5) is coated with a mouse anti-swine SC protein monoclonal antibody, and the detection line (5) is arranged on the side near to the sample pad (3); the quality control line (6) is coated with a purified protein of a positive serum of African swine fever, and the quality control line (6) is arranged on the side near to the water absorption pad (7). A sample diluent contains recombinant polypeptide tandem protein R10 and recombinant protein P30V labeled by a quantum dot microsphere; the sequences of R10 and P30V are as shown in SEQ ID NO: 2 and 4, respectively. Use the test strip (1) in coordination with the sample diluent, for use in rapid, high-specificity, and high-sensitivity qualitative detection of an African swine fever virus mucous membrane sIgA antibody.
The disclosure discloses a Chinese cabbage plasma membrane intrinsic aquaporin, having an amino acid sequence as set forth in SEQ ID NO. 2, and the nucleotide sequence of the encoding gene BraPIP1;1 thereof is as set forth in SEQ ID NO. 1. The plasma membrane intrinsic aquaporin has the characteristic of sensitively responding to neonicotinoid insecticides (thiamethoxam, imidacloprid, etc.) in the external environment. At the same time, it has the function of mediating transmembrane transport of the neonicotinoid insecticides, promoting accumulation of the neonicotinoid insecticides in plant roots and leaves, which has important application value in guiding efficient and simple use of pesticides, development of new systemic pesticides, etc.
Provided are an abnormal cotton chromosome fragment capable of causing lethality of upland cotton and a molecular marker. The chromosome fragment A11-9 is from an abnormal cotton, is located on the chromosome 11 of a cotton genome, and is marked by six pairs of SSR markers: NAU5192, A11_175, JAAS3191, A11_243, JAAS3310 and A11_193;by taking DNA of the abnormal cotton as a template, the six pairs of SSR markers are simultaneously used to amplify the DNA of the abnormal cotton, and the chromosome fragment containing a target band of all the six pairs of SSR markers is the chromosome fragment A11-9 of the abnormal cotton. Further provided is a single-fragment introgression line derived from the lethal traits of abnormal cotton, which creates an important material for promoting fine positioning of a target gene and subsequent map-based cloning.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
70.
METHOD FOR PROCESSING COLD FRESH POULTRY THAT IMPROVES MEAT QUALITY, AND PRODUCT
A method for processing cold fresh poultry that improves meat quality, and a product. The method comprises the following steps: step 1, performing quick microwave heating on a slaughtered and cleaned poultry carcass such that the central temperature of the poultry carcass reaches 45-55°C; step 2, placing the poultry carcass from step 1 in 60°C low-temperature steam, and keeping warm for 20-30 minutes; and step 3, wind cooling the poultry carcass after processing in step 2 until the central temperature reaches 15°C, then grading and packaging the poultry carcass. The described method for processing cold fresh poultry that improves meat quality can be linked with a traditional poultry slaughtering line, thus the quality of cold fresh poultry meat is effectively improved, cooking loss is reduced, all viruses and some bacteria are killed, and the shelf life of the product is improved. The produced cold fresh poultry meat has good toughness, is chewy, is close to fresh poultry meat in mouthfeel, and is very suitable for Chinese cuisine.
A method for efficiently enriching lutein from broccoli seedlings using γ-aminobutyric acid and sodium chloride stress in combination, comprising: taking Qingfeng variety of broccoli seeds having full grains, uniform sizes, and germinating capability as raw materials, removing impurities, performing disinfection and sterilization using 0.5% of NaClO, then shifting the raw materials to an incubator of 25℃ for germinating under 16 h light/8 h dark condition, spraying 0.25-1.00 mmol/L of γ-aminobutyric acid and 100 mM of NaCl water solution in combination after distilled water is sprayed for one day, and harvesting the broccoli seedlings four days later. By means of the method, the gene expression amounts of ZEP and VDE are respectively increased by 0.87-2.41 times and 1.14-2.75 times, and moreover, the content of the lutein in the broccoli seedlings is remarkably increased, thereby effectively enhancing the function of the broccoli seedlings and achieving broad application prospect.
A production method for a special poultry meat raw material for soup and stew food, relating to the technical field of food processing. According to the method, poultry passing inspection and quarantine undergoes slaughter and blood draining, defeathering, eviscerating, dicing, washing, draining, treatment with superheated steam, air cooling, individual quick freezing and storage at -18°C, and is finally prepared into a special poultry raw material required for industrial production of soup and stew food. The method retains umami substances of the poultry meat raw material to the greatest extent, guarantees the feature of savory taste of the final soup and stew food, solves the existing problems of poor meat quality and insufficient umami generally existing in products produced by frozen raw materials, and provides a technical foundation for industrialization of soup and stew food.
Disclosed are a rapid test card for simultaneously detecting porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV), and a preparation method and a use method therefor. The rapid test card comprises a card shell (1) and a test strip (2) arranged in the card shell. The test strip (2) comprises a PVC bottom plate, and a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad which are sequentially connected and pasted to the PVC bottom plate. A sample adding hole (3) and a result observation window (4) are formed in the card shell (1). An anti-PEDV specific monoclonal antibody and an anti-TGEV specific monoclonal antibody are fixed on the nitrocellulose membrane to form a first detection line (T1) and a second detection line (T2), respectively; and the anti-PEDV specific monoclonal antibody marked by colloidal gold and the anti-TGEV specific monoclonal antibody marked by colloidal gold are fixed on the combination pad. With only one instance of sample adding, the rapid test card can be used to simultaneously detect PEDV and TGEV in a pig excrement sample, and reactions are performed under unified conditions; and the rapid test card has advantages such as rapidness, high accuracy, high sensitivity, and simple operation.
A pig production method by using a detachable and washable wet curtain for intensive pig farming The detachable and washable wet curtain includes a wet curtain mounting frame (1), a spray tube (4), a plurality of rotatable reels (7) equivalent to sleeves, a reel (6) with a buckle, a wet curtain net (8), a reel (10) with a buckle, and a manual handle (9). The wet curtain is formed by a low-cost common material, namely a plastic net and the like, is convenient to mount, detach, and wash, and is not prone to making dust, scale, algae, mosquitos, and the like accumulate thereon, thus reducing the replacement cost and use cost. Therefore, a new cooling method based on the wet curtain is provided for pig farms in summer, and it is also suitable for other livestock farms in summer.
A bok choy plasma membrane intrinsic aquaporin having an amino acid sequence as shown in SEQ ID NO. 2, and the nucleotide sequence of an encoding gene BraPIP1;1 thereof being as shown in SEQ ID NO. 1. The plasma membrane intrinsic aquaporin has the feature of sensitive response to neonicotinoid insecticides (thiamethoxam, imidacloprid, etc.) in the external environment, while also having the function of mediating transmembrane transport of neonicotinoid insecticides, thus promoting accumulation of a neonicotinoid insecticide in the stems and leaves of a plant, and having great value for application in guiding efficient and light use of pesticides and development of novel systemic pesticides.
A bok choy plasma membrane intrinsic aquaporin having an amino acid sequence as shown in SEQ ID NO. 2. The nucleotide sequence of an encoding gene BraPIP1;1 thereof is as shown in SEQ ID NO. 1, and the plasma membrane intrinsic aquaporin has the feature of sensitive response to neonicotinoid insecticides (thiamethoxam, imidacloprid, etc.) in the external environment, while also having the function of mediating transmembrane transport of neonicotinoid insecticides, thus promoting accumulation of a neonicotinoid insecticide in the stems and leaves of a plant, and having great value for application in guiding efficient and light use of pesticides and development of novel systemic pesticides.
An Aeromicrobium reducing zearalenone and a use therefor, said HA-1 (Aeromibrobium tamlense) strain having an accession number of: CGMCC No. 19892, and a 16S rDNA nucleotide sequence thereof being as shown in SEQ ID NO.1. The HA-1 strain may be used in reducing zearalenone. In a process of resolving toxin contamination, reduction is total, with no secondary contamination, thereby improving the quality level and safety level of agricultural products and feedstuff, ensuring food safety for humans and livestock.
Provided are a nanoliposome containing lutein and Cordyceps militaris alcohol extract, and a method for preparation thereof; the contained amount of Cordyceps militaris alcohol extract is 4%–8% by mass, and the mass ratio of lutein to Cordyceps militaris alcohol extract is 1:3–1:5. An ethanol injection method is combined with micro-jet technology to achieve co-loading of lutein and Cordyceps militaris alcohol extract, two active substances having different hydrophobicities, in a nanoliposome; the prepared liposome has a particle size of less than 200 nm, the loading rate of lutein is higher than 90%, the loading rate of Cordyceps militaris alcohol extract is higher than 85%, and stability is good; the preparation process is safe and simple, costs are low, facilitating industrial production.
A23L 33/00 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof
A23P 10/47 - Making free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added using additives, e.g. emulsifiers, wetting agents or dust-binding agents
A61K 9/127 - Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
A61K 31/047 - Hydroxy compounds, e.g. alcoholsSalts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
A61P 9/00 - Drugs for disorders of the cardiovascular system
1-xx21-xx21-xx221-xx221-xx222/PNIPAM composite solution. The material of the present invention has the advantages of high visible light transmittance, high variable temperature spectrum regulation efficiency, high photo-thermal stability, high adaptability, etc.
JIANGSU YINBAO AGRICULTURAL SCIENCE RESEARCH INSTITUTE CO., LTD. (China)
Inventor
Guan, Qingbao
You, Zhengwei
Gao, Yi
Zhao, Yang
Xu, Lei
Yan, Nina
Abstract
A light-curing three-dimensional printing preview method, comprising the following steps: obtaining the relationship curves of the conversion rate of a photosensitive resin system versus time under different curing conditions by means of measuring the relationship between the absorbance of the photosensitive resin system under different curing conditions and the time; conducting fitting on experimental curves on the basis of a light-curing kinetic equation by means of an iterative solution method, and obtaining a light-curing kinetic constant of the photosensitive resin system; solving a light-curing reaction kinetic differential equation set by using a fixed-step Euler method, and obtaining the relationship curves of the space distribution of each component content of the photosensitive resin system in the light-curing reaction process versus time; and conducting simulation printing, in conjunction with the relationship curves of the space distribution of each component content versus time, obtaining a space distribution of each component content in each layer of structure in a three-dimensional printing product after printing is completed, and completing a light-curing three-dimensional printing preview, thereby filling the gap of three-dimensional printing control software in respect of the preview function.
B29C 64/124 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
B29C 64/386 - Data acquisition or data processing for additive manufacturing
B29C 64/393 - Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
B33Y 30/00 - Apparatus for additive manufacturingDetails thereof or accessories therefor
B33Y 50/00 - Data acquisition or data processing for additive manufacturing
B33Y 50/02 - Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
G01N 21/35 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
81.
Method for creating new germplasm of male sterile crop by gene editing and application thereof
A method for creating a new germplasm of a male sterile crop by gene editing and an application thereof are provided. In this method, the gene editing is performed on an exon region of a Ty-5 gene, and a deletion of DNA sequence is introduced by using a repair mechanism of plants themselves to double-strand breaks (DSBs), causing a loss of function of Ty-5 gene, thereby obtaining a male-sterile character. The method can be applied without being limited by crop categories. After the gene editing is performed on Ty-5 genes of various crops, new germplasms can be quickly obtained. The new germplasms have the same agronomic characters as the previous materials, and only differ in sexual aspect, which effectively solves the problem of the lack of male sterile materials and unstable fertility in natural resources.
Disclosed are a method for enriching lutein with germinated corn, germinated corn prepared on the basis of the method, and the use of the germinated corns in the preparation of food, drugs, health care products or cosmetics containing lutein. The method comprises the following steps: taking corn grains which have the ability to germinate as raw materials, disinfecting same by means of a sodium hypochlorite solution, then sequentially conducting seed soaking in a methyl jasmonate solution and a sodium chloride solution, then culturing the corn grains in the dark at 25 to 28℃ for 48 to 96 hours, and spraying the methyl jasmonate solution every 4 to 6 hours during germination. By means of determination, it can be seen that the method can improve the lutein content of germinated corns by at least 50％, and the zeaxanthin content of germinated corns by at least 40％.
The present invention relates to the preparation of the compound immunopotentiator and the application thereof in a foot-and-mouth disease vaccine of pigs. According to the present invention, the foot-and-mouth disease vaccine of pigs is taken as a research subject, and on this basis, several immunopotentiators having obvious immunopotentiating effects are selected for the compound immunopotentiator, and an antigen/vaccine is mixed with the immunopotentiator to prepare a vaccine-immunized pig. An animal experiment result shows that the present invention has obvious immunopotentiating effects. After immunizing the vaccine containing the compound immunopotentiator, a window period for antibody production can be significantly shortened to 7 days; a LPB-ELISA antibody titer is significantly improved, and an antibody pass rate is significantly increased; an immune protection period is also significantly extended, at least up to 7 months.
A method of using magnetic nano material to purify plant anthocyanin. The prepared magnetic nano material has a particle size of 10-600 nm, and can effectively adsorb a coloring agent and enhance the stability thereof. Using a magnetic nano material as an adsorption vector facilitates separation and re-use of the material. The method allows for targeted and controllable release, is able to achieve high purification and separation effectiveness, is easy to carry out, and is suited for use in the adsoprtion and purification of plant anthocyanin having various structures.
B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
B01J 20/30 - Processes for preparing, regenerating or reactivating
C07D 311/62 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulfur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
85.
GOSSYPIUM ANOMALUM-SOURCED SSR SEQUENCE ASSOCIATED WITH HIGH LINT PERCENTAGE AND DROUGHT TOLERANCE AND APPLICATION THEREOF
Provided is a gossypium anomalum-sourced SSR sequence associated with high lint percentage and drought tolerance and an application thereof. Provided is a gossypium anomalum-sourced chromosome segment which is a DNA segment from an SSR molecular marker JAAS6365 to an SSR molecular marker JAAS5604 on chromosome 5 of the gossypium anomalum. The nucleotide sequence of the SSR molecular marker JAAS6365 is shown as SEQ ID No. 1, and the nucleotide sequence of the SSR molecular marker JAAS5604 is shown as SEQ ID No. 2.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
86.
Zea mays NLP transcription factor ZmNLP5 and use thereof
The present invention clones a gene ZmNLP5 from maize, which plays an important regulatory role in nitrogen assimilation, and the open reading frame of which has a DNA sequence shown as SEQ ID NO:1. The transcription factor protein encoded by the ZmNLP5 gene has an amino acid sequence shown as SEQ ID NO:2. The uses of the maize NLP transcription factor ZmNLP5 mentioned above in promoting expression of a nitrogen metabolic key enzyme gene ZmNIR1.1, in promoting expression of a nitrogen metabolic key enzyme gene ZmNIR1.2, in promoting expression of a nitrogen metabolic key enzyme gene ZmNR1.1, in promoting expression of a nitrogen metabolic key enzyme gene ZmNR1.2, in improving nitrogen assimilation in maize, and in promoting elongation growth of maize root in deficient nitrogen environment are further provided.
A preparation method for a combined modified straw active particulate carbon adsorption material and use of same. The preparation method for the combined modified straw active particulate carbon adsorption material comprises the following steps: 1) mixing straw powders, distilled water, a binder and a composite mineral, then pelletizing same, and then placing same in a tube furnace for pyrolysis to prepare straw particulate carbon; 2) introducing an inert gas into a modification reagent, adjusting the pH value combined and 3) soaking the straw particulate carbon into the combined modification solution for 30 min, and performing cleaning and drying, so as to obtain a combined modified straw active particulate carbon adsorption material. The combined modified straw active particulate carbon has a good adsorption effect on phosphate group in low-pollution water.
C01B 32/318 - Preparation characterised by the starting materials
B01J 20/20 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising free carbonSolid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising carbon obtained by carbonising processes
C05G 3/40 - Mixtures of one or more fertilisers with additives not having a specifically fertilising activity for affecting fertiliser dosage or release rateMixtures of one or more fertilisers with additives not having a specifically fertilising activity for affecting solubility
B01J 20/12 - Naturally occurring clays or bleaching earth
B01J 20/30 - Processes for preparing, regenerating or reactivating
C02F 1/28 - Treatment of water, waste water, or sewage by sorption
A bruchid harm condition observation technique based on a spectral imaging technique, relating to the technical field of plant disease and pest harm study. A method may comprise: randomly selecting beam seeds; leaving the seeds to stand on a plane; obtaining the spectral image of the seeds by using a spectral imaging system; obtaining an image under the characteristic wavelength of F440 or F520, and Numeric Avg by using FluorCam7 Software; and by combining the image and data, analyzing and determining the bruchid harm condition of the batch of seeds. By means of the method, the present invention is easy to operate, wide in applicability, and capable of realizing mass process, reduces a manual determination error, and is precise in determination.
The present invention involves cloning a gene ZmNLP5 from Zea mays that plays an important regulatory role in nitrogen nutrition assimilation and utilization, wherein the open reading frame of the gene has the DNA sequence as shown in SEQ ID NO: 1. A transcription factor protein encoded by the ZmNLP5 gene has the amino acid sequence as shown in SEQ ID NO: 2. Also provided are the uses of the above-mentioned Zea mays NLP transcription factor ZmNLP5 in promoting the expression of a key enzyme gene ZmNIR1.1 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNIR1.2 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNR1.1 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNR1.2 for nitrogen metabolism, in improving the nitrogen nutrition assimilation and utilization by Zea mays, and in promoting the elongation and growth of corn roots in low nitrogen environments. The Zea mays NLP transcription factor ZmNLP5 of the present invention can promote the expression of key enzyme genes for nitrogen metabolism, improve the nitrogen nutrition assimilation and utilization by Zea mays, and promote the elongation and growth of corn roots in low nitrogen environments, and is suitable for large-scale popularization and application.
A yellow-feathered chicken material production method applicable to a Chinese food cooking mode and a chilled fresh yellow-feathered chicken product, the material production method comprising the following steps: shaping a butchered and cleaned yellow-feathered chicken, without carrying out acid discharge tenderization treatment; and directly performing water bath blanching enzyme deactivation in warm water at 50-60°C for 20-30 min. The described processing method for the chilled fresh yellow-feathered chicken has the advantages of temperature reduction acid discharge and tenderization treatment not being performed in the processing process; the processing method may overcome the defect of water easily being generated in the stir-frying and stewing process of the chilled fresh yellow-feathered chicken, the steaming and boiling loss is effectively reduced, the quality of the meal is high, and a dry mouth feeling will not be produced when chewing. In the product storage process, the water loss is reduced, and there is little loss of moisture.
A61K 36/00 - Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
A01N 65/34 - Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
92.
Human-derived insecticidal bt cry toxin mimetic, and design method and application thereof
Plutella xylostella indoor insecticidal biological activity assay, the insecticidal protein shows a good insecticidal effect, and can effectively replace Cry1Ac or Cry1Ab toxin for biological control of insect pest.
A porous biomass adsorbing material, and a preparation method and application thereof. The porous biomass adsorbing material is prepared by the following method: irradiating a biomass material in air with no less than 10 kGy of irradiation dose; dispersing the irradiated biomass material in a mixed aqueous solution of sodium hydroxide and urea or a mixed aqueous solution of sodium hydroxide and thiourea, stirring at a normal temperature after freezing, and filtering to obtain a biomass sol; subjecting the biomass sol to gelatinization to obtain a biomass gel, and washing to neutral to obtain an amidated porous biomass adsorbing material. The biomass adsorbing material has the advantages that no post-modification is required, an amino group can be introduced into a main chain of a biomass molecule by one step, functionalization of the porous biomass material is implemented, and purification of high-chroma wastewater of industries and food can be implemented.
Disclosed are a rice ALS mutant-type protein, a mutant-type gene and the use thereof, wherein the amino acid sequence of the ALS mutant-type protein contains the following mutation: there is mutation of an amino acid at position 628 corresponding to the amino acid sequence of the rice ALS. Further disclosed is a breeding method for creating herbicide-resistant rice by means of gene editing.
Provided are a rape gene which is resistant to pyrimidine salicylic acid herbicides and the use thereof, and further provided are a rape plant and parts thereof which can withstand pyrimidine salicylic acid herbicides.
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 6/20 - Brassicaceae, e.g. canola, broccoli or rucola
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
A rapeseed plant tolerant against a triazole pyrimidinesulfonamide herbicide, parts thereof, a resistance gene, a mutant protein, and an application thereof.
Provided are a rape gene which is resistant to pyrimidine salicylic acid herbicides and the use thereof, and further provided are a rape plant and parts thereof which can withstand pyrimidine salicylic acid herbicides.
Provided is a method for enhancing virus proliferation, and a BHK21 cell population the innate immune system of which is remodeled is constructed, being used for virus proliferation. The present method opens up new ideas for virus proliferation, expands the application range of BHK21 cells, and lays a solid foundation for the development of vaccines capable of preventing and treating viruses.
A malic acid and KMnO4-based combined and modified cow dung biogas residue hydrochar preparation method, comprising: mixing a cow dung biogas residue with malic acid, and performing ultrasonic treatment to obtain a malic acid modified cow dung biogas residue; performing a hydrothermal reaction with KMnO4 in a high-temperature high-pressure reactor to obtain a combined and modified cow dung biogas residue hydrochar material.
B01J 20/20 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising free carbonSolid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising carbon obtained by carbonising processes
B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
C01B 32/05 - Preparation or purification of carbon not covered by groups , , ,
C02F 1/28 - Treatment of water, waste water, or sewage by sorption
A method employing gene editing to create a new germplasm of a sterile male crop and an application thereof. In the method, gene editing is performed in the exon region of Ty-5 and a plant's own DBS repair mechanism is used to introduce a deletion into a DNA sequence, such that Ty-5 gene function is lost, thereby obtaining the sterile male characteristics.
patents
