The present invention relates to a modified α-amino acid ester hydrolase with increased amoxicillin productivity, and a use thereof. The modified AEH of the present invention is highly industrially applicable since the modified AEH has a high synthetic yield of amoxicillin(Amox) as well as a remarkably low decomposition rate of HPGM, thereby significantly increasing productivity of amoxicillin.
A method for preparing collagenase of the present invention comprises: a first step for centrifuging a Bacillus subtilis strain; a second step for concentrating the centrifuged supernatant; and a third step for purifying the supernatant using ion exchange chromatography. A method for preparing a collagen tripeptide comprises: a first step for mixing pre-treated fish scales and water at a weight ratio of 2:8; a second step for heat-treating the mixture at 90°C for 5 hours; a third step for adding the collagenase prepared by the above-described method, followed by degradation at 35°C for 12 hours; a fourth step for removing foreign materials from the component in the third step through centrifugation; a fifth step for purifying the component by ion exchange chromatography; a sixth step for concentrating the purified component; a seventh step for purifying the concentrated component using activated carbon; and an eighth step for removing microorganisms from the component using a filter.
C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
29 - Meat, dairy products, prepared or preserved foods
Goods & Services
Health functional food, with glucosamine base, extracted
from crustacean shells; health functional food, with
glucosamine base, extracted from mollusk bones; health
functional food, with n-acetylglucosamine base, extracted
from crustacean shells; health functional food, with
n-acetylglucosamine base, extracted from mollusk bones;
health functional food, with chitosan base, extracted from
crustacean shells; health functional food, with chitosan
base, extracted from mollusk bones; health functional food,
with chitooligosaccharide base, extracted from crustacean
shells; health functional food, with chitooligosaccharide
base, extracted from mollusk bones; health functional food,
with anthocyanin base, extracted from fruit peels; health
functional food, with collagen base, extracted from fish
scales; health functional food, with collagen peptide base,
extracted from fish scales; health functional food, with
collagen base, extracted from pig skins; health functional
food, with collagen peptide base, extracted from pig skins;
health functional food, with fucoxanthin base, extracted
from seaweed; lactobacillus-based health functional food;
health functional food, with calcium base, in aquatic
products; health functional food, with calcium base, in
seaweed; health functional food, with pycnogenol base,
extracted from pine bark.
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C12P 7/00 - Preparation of oxygen-containing organic compounds
C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
5.
METHOD OF PRODUCING LYCOPENE USING RECOMBINANT ESHERICHIA COLI
A method of producing ljcopene, with high productivity by means of a recombinant bacterial strain includes preparing the recombinant vector containing genes encoding proteins, which are required for ljcopene biosynthesis. The genes involved in ljcopene biosynthesis are crtE, crtB and crtl, and at least one of the said three genes (crtE, crtB and crtI) is selected from the group consisting of crtE with the nucleotide Sequence 1, crtB with the nucleotide Sequence 3 and crtl with the nucleotide Sequence 5, of the Sequence List. The said recombinant vector is transformed into Escherichia coli (hereafter E. coli). The E. coli transformant is cultured to recover ljcopene from the culture medium.
There are provided genes involved in the biosynthesis of lycopene and having DNA sequences set forth in SEQ ID NO: 1, SEQ ID NO: 3 and SEQ ID NO: 5 encoding proteins required for the biosynthesis of lycopene, a recombinant vector comprising at least one of the genes, and a mi¬ croorganism transformed with the recombinant vector and having a high content of lycopene. The lycopene is obtained at a yield of 15.3 mg/L and a content of 4.2 mg/gDCW when the recombined E. coli with the crt genes is cultivated, and the lycopene is also obtained with the maximum content of 5.4 mg/gDCW when a microorganism is transformed with the combination of the gene of the present invention and the known genes. Therefore, provided is the lycopene- producing strain having a more increased content of lycopene per dry cell weight than the known lycopene-producing strain with the genes. Accordingly, the genes may be useful to mass-produce lycopene in microorganisms, and also to mass-produce carotenoids.
