Abstract

A method for the determination of pollen viability and/or maturation grade of a pollen population, comprising mechanically removing pollen grains from flowers; re-suspending pollen grains in an electrically conductive buffer for keeping the plant cells to remain viable; passing the pollen suspension through an appropriate filter with a pore size suitable for a microfluidic device being adapted to perform impedance flow cytometry (IFC); An advantage of the present invention is that a plant cell does not have to be stained for viability analysis, the method is non-invasive and a high number of cells can be analyzed in a short time frame in real-time and on-site. It is possible to follow developmental processes of plant cells, and that it is applicable to all plant species. In particular, the method allows a standardized measurement independent of the location and all over the world.

IPC Classes  ?

• G01N 27/20 - Investigating the presence of flaws
• G01N 33/483 - Physical analysis of biological material
• A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
• A01H 1/04 - Processes of selection

Abstract

A method is provided for the discrimination of cells from other particles, as well as of different types of cells in raw milk samples by impedance microflow cytometry. A method is provided of analysing the quality of raw milk in terms of its bacterial and somatic cell content without the need to pre-treat the milk sample, such that the analysis can take place directly at the production site. One advantage is discriminating and counting somatic cells from milk particles (consisting mainly of lipid vesicles) by high-frequency impedance analysis directly performed on untreated raw milk. Another advantage is that the method allows diagnosing the status of a mastitis infection directly after milking according to the analysis of the somatic cell count. Another advantage is to allow a fast method for determining the bacterial cell count in raw milk directly after milking. In addition, viability of both somatic and bacterial cells can be determined without the need of any cell label. A further advantage is, that the analysis can be obtained in real-time, directly after the raw milk has passed the micro channel of the microfluidic device.

IPC Classes  ?

• G01N 33/04 - Dairy products
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• A01J 5/013 - On-site detection of mastitis in milk
• G01N 15/10 - Investigating individual particles

Goods & Services

Chips (wafer semiconductor material on which are placed microelectronic circuit components and circuits); sensors (measuring apparatus); software, particularly for testing apparatus for medical use, particularly biomedical. Surgical, medical, dental and veterinary apparatus and instruments; testing apparatus for medical use as well as their accessories and spare parts. Scientific and technological services and research and design relating thereto; design and development of testing methods; scientific analyses, particularly in the field of medicine, particularly biomedicine; scientific analysis and research services, particularly in the field of medicine, particularly biomedicine; scientific consultancy services in connection with technical, scientific and medical analyses, particularly in the field of biomedicine; provision of scientific information in connection with technical, scientific and medical research and analysis, particularly on the Internet; design and development of computer hardware and software; provision of software applications via the Internet, particularly for analyses, particularly in the field of medicine, particularly biomedicine; research and development services in connection with micro-organisms.

Goods & Services

Chips (wafer semiconductor material on which are placed microelectronic circuit components and circuits); sensors (measuring apparatus); software, particularly for testing apparatus for medical use, particularly biomedical. Surgical, medical, dental and veterinary apparatus and instruments; testing apparatus for medical use as well as their accessories and spare parts. Scientific and technological services and research and design relating thereto; design and development of testing methods; scientific analyses, particularly in the field of medicine, particularly biomedicine; scientific analysis and research services, particularly in the field of medicine, particularly biomedicine; scientific consultancy services in connection with technical, scientific and medical analyses, particularly in the field of biomedicine; provision of scientific information in connection with technical, scientific and medical research and analysis, particularly on the Internet; design and development of computer hardware and software; provision of software applications via the Internet, particularly for analyses, particularly in the field of medicine, particularly biomedicine; research and development services in connection with micro-organisms.

Goods & Services

[ Semiconductor wafer chips featuring microelectronic circuit components and circuits; sensors for measuring impedance in biological cells; computer software for control of fluidics, data acquisition, signal processing and data analysis ] Medical apparatus and instruments for analysis of biological cells [ Scientific and technological services, namely, analysis of biological cells and cell cultures; data-analysis of measurements performed on biological cells; scientific consulting in the field of analysis of biological cells ]

Goods & Services

Semiconductor wafer chips featuring microelectronic circuit components and circuits; sensors for measuring impedance in biological cells; computer software for control of fluidics, data acquisition, signal processing and data analysis Medical apparatus and instruments for analysis of biological cells Scientific and technological services, namely, analysis of biological cells and cell cultures; data-analysis of measurements performed on biological cells; scientific consulting in the field of analysis of biological cells