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Found results for
 patents
1.
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USE OF AAV REPLICATION MACHINERY FOR IMPROVED PROTEIN PRODUCTION
| Application Number |
NL2008050613 |
| Publication Number |
2009/038462 |
| Status |
In Force |
| Filing Date |
2008-09-18 |
| Publication Date |
2009-03-26 |
| Owner |
AMSTERDAM MOLECULAR THERAPEUTICS B.V. (Netherlands)
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| Inventor |
- Hermens, Wilhelmus, Theodorus, Johannes, Maria, Christiaan, Hermens
- Noordman, Yvet
- Bakker, Andrew Christian
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Abstract
The present invention provides a method for enhanced production of a gene product of interest in a cell, using the AAV replication machinery. The present invention further relates to a cell for use in the method of the invention and a non- human transgenic animal or a transgenic plant comprising a cell of the invention.
IPC Classes ?
- C12N 15/866 - Baculoviral vectors
- C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
- C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
- A01K 67/027 - New or modified breeds of vertebrates
- C07K 14/015 - Parvoviridae, e.g. feline panleukopenia virus, human parvovirus
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2.
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BACULOVIRAL VECTORS COMPRISING REPEATED CODING SEQUENCES WITH DIFFERENTIAL CODON BIASES
| Application Number |
NL2008050512 |
| Publication Number |
2009/014445 |
| Status |
In Force |
| Filing Date |
2008-07-25 |
| Publication Date |
2009-01-29 |
| Owner |
AMSTERDAM MOLECULAR THERAPEUTICS B.V. (Netherlands)
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| Inventor |
- Bakker, Andrew, Christian
- Hermens, Wilhelmus, Theodorus, Johannes, Maria, Christiaan
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Abstract
The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors.
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3.
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VECTORS WITH MODIFIED INITIATION CODON FOR THE TRANSLATION OF AAV-REP78 USEFUL FOR PRODUCTION OF AAV IN INSECT CELLS
| Application Number |
NL2007050298 |
| Publication Number |
2007/148971 |
| Status |
In Force |
| Filing Date |
2007-06-20 |
| Publication Date |
2007-12-27 |
| Owner |
AMSTERDAM MOLECULAR THERAPEUTICS B.V. (Netherlands)
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| Inventor |
- Hermens, Wilhelmus, Theodorus, Johannes, Maria, Christiaan
- Haast, Saskia, Jacoba, Petronella
- Biesmans, Dennis, Johan
- Bakker, Andrew, Christian
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Abstract
The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
IPC Classes ?
- C12N 15/864 - Parvoviral vectors
- C07K 14/015 - Parvoviridae, e.g. feline panleukopenia virus, human parvovirus
- C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
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4.
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IMPROVED AAV VECTORS PRODUCED IN INSECT CELLS
| Application Number |
NL2006050262 |
| Publication Number |
2007/046703 |
| Status |
In Force |
| Filing Date |
2006-10-19 |
| Publication Date |
2007-04-26 |
| Owner |
AMSTERDAM MOLECULAR THERAPEUTICS B.V. (Netherlands)
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| Inventor |
- Urabe, Masashi
- Ozawa, Keiya
- Haast, Saskia, Jacoba, Petronella
- Hermens, Wilhelmus, Theodorus, Johannes, Maria, Christian
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Abstract
The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon. The insect cell further comprises a second nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence; a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence operably linked to expression control sequences for expression in an insect cell; and, a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to expression control sequences for expression in an insect cell. The invention further relates to adeno-associated viral vectors with an altered ratio of the viral capsid proteins that provides improved infectivity o f the viral particles.
IPC Classes ?
- C12N 15/864 - Parvoviral vectors
- C12N 15/866 - Baculoviral vectors
- C12N 15/35 - Parvoviridae, e.g. feline panleukopenia virus, human parvovirus
- C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
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