Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Detection Center of Microbiology (China)
Inventor
Xu, Meiying
Zhang, Jinna
Wang, Ariguo
Lai, Sihua
Abstract
A method for simultaneous and efficient detection of residues of 36 antidepressants in sludge includes extraction of target drugs, purification of an extraction solution, detection by the technique of ultra-performance liquid chromatography-tandem mass spectrometry, and qualitative and quantitative analysis of the antidepressants. Based on the detection technique of ultra-performance liquid chromatography-tandem mass spectrometry, by using UPLC/QTOF-MS in combination with pretreatment methods. The method solves the problems such as absence of detection methods for some antidepressants in existing detection methods, high time consumption of sample pretreatment experiments, high detection costs, and low resolution of detection methods. According to the method, the recovery rate of antidepressants is 63.13-115.66%, and the limit of detection of the method is 0.14-13.47 ng/g; only 0.5 g of dry sludge sample is required. The experimental process is simplified, the introduced random errors are reduced, and the time and detection cost consumed by the experiment are greatly reduced.
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Cui, Zongbin
Lu, Xing
Abstract
Use of Lactobacillus plantarum in a preparation for promoting the sugar utilization ability of a fish. The probiotic is Lactobacillus plantarum. The probiotic can adjust a liver PPAR signaling pathway and a tricarboxylic acid cycle pathway by means of maintaining the intestinal flora homeostasis in Micropterus salmoides, so that the glucose and lipid metabolism disorders in the liver of Micropterus salmoides caused by high-sugar diet feeding is alleviated and the antioxidant capacity of the liver of Micropterus salmoides is improved. A new solution is provided for improving the sugar tolerance and healthy breeding of carnivorous fishes.
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY (China)
Inventor
Kou, Xiuying
Wu, Xiaoxian
Tang, Jian
Wu, Qingping
Hu, Huiping
Liang, Xiaowei
Cai, Manjun
Xie, Yizhen
Liu, Yuanchao
Zhuo, Lijun
Wang, Ao
Du, Na
Abstract
The present invention relates to a molecular marker, a specific primer pair, and an identification method of the high-quality Ganoderma lucidum strain HMGIM-M624. The high-quality Ganoderma lucidum strain HMGIM-M624 was preserved in Guangdong Microbial Culture Collection Center (address: 5th Floor, No. 59 Building of No. 100 Yard, Mid. Xianlie Road, Guangzhou City) with the preservation number of GDMCC No: 60889 on Nov. 7, 2019. The molecular marker is an InDel molecular marker. The high-quality Ganoderma lucidum strain HMGIM-M624 has a base deletion of CATGCTGTA at the 246451th-246460th site of the chromosome sca34. The present invention provides reagents for detecting the molecular marker of the high-quality Ganoderma lucidum strain HMGIM-M624. The reagents can distinguish the high-quality Ganoderma lucidum strain HMGIM-M624 from the other 11 G. lucidum strains for commercial cultivation, thus specifically identifying the high-quality Ganoderma lucidum strain HMGIM-M624.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
4.
NEW TYPE OF EFFICIENT FLOCCULATION DENITRIFYING BACTERIA AND USE THEREOF
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Zhu, Honghui
Zhang, Mingxia
Chen, Meibiao
Zhang, Yulian
Yang, Fan
Yao, Qing
Abstract
Provided are a new type of efficient flocculation denitrifying bacteria and the use thereof. Thauera sp. JM12B12 is separated and screened from water in a Penaeus vannamei culture pond, and was preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 27, 2023, the address thereof being Building 59, Yard 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, and the preservation number being GDMCC No. 63587. Thauera sp. JM12B12 is separated from water in a Penaeus vannamei culture pond, namely, the removal rates of nitrate nitrogen and nitrite nitrogen by the strain are both 100% when the carbon-nitrogen ratio is 5-20. In addition, the strain also has an efficient flocculation function, namely when nitrate nitrogen is removed, the flocculation efficiency of the bacteria cells thereof and the supernatant is 90.6% and 90.1% respectively. The new efficient flocculation denitrifying bacteria JM12B12 provided has important application value in denitrification and removal of solid suspended matter of wastewater exceeding the standard of nitrogen content, thereby providing a new microbial material for water environment protection.
INSTITUTE OF MICROBIOLOGY , GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Zhang, Jinna
Xu, Meiying
Lai, Sihua
Wang, Anguo
Abstract
Provided are a high-efficiency levonorgestrel-degrading strain and the use thereof. The strain is Gordonia sp. H52 with the deposit number GDMCC No: 62995. The strain degrades levonorgestrel at a fast rate, can be used for degrading levonorgestrel alone, and can also be used for degrading mixed pollutants of various synthetic steroid hormones.
C02F 3/34 - Biological treatment of water, waste water, or sewage characterised by the microorganisms used
A62D 3/02 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Xu, Meiying
Liao, Bing
Luo, Yeshen
Abstract
The present invention discloses a GSH-based fluorescent nanoprobe, and a synthesis method therefor and the use thereof. The present invention first relates to the synthesis method, comprising: preparing a graphene quantum dot with a surface rich in hydroxyl groups by means of the polymerization of a micromolecular polycyclic aromatic hydrocarbon using a bottom-up strategy, then grafting carboxylic acid groups to enable the surface of the graphene quantum dot to be rich in carboxyl groups, and finally, modifying the surface of the graphene quantum dot with a recognition unit to obtain a specific graphene quantum dot fluorescent probe. The fluorescent nanoprobe prepared by using the method structurally exhibits excellent fluorescence characteristics and specificity, and can be used as a fluorescent substrate for drug delivery, biomedical imaging, ultra-sensitive biosensors, microbiological detection, etc.
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Xu, Meiying
Yang, Shan
Lin, Lizhou
Dong, Meijun
Yang, Xunan
Abstract
A river ecosystem health evaluation method based on microbial community specific response. The method comprises: a: respectively setting sampling points of rural areas, urban suburban areas and urban city centers along a river; b: measuring the water body to determine 12 indicators of pH, water temperature (WT), dissolved oxygen (DO), COD, TN, total organic carbon, nitrate nitrogen, ammonium nitrogen, nitrite nitrogen, total phosphorus, sulfide and fluoride, and calculating a water quality index (IWQ); c: extracting microbial DNA from the water body and carrying out bacterial 16s rRNA amplicon sequencing; d: obtaining a bacterial genus level information table, and screening for aeffective bacterial genus information; e: performing microbial sensitivity division on the basis of an ecological level model; and f: performing evaluation result reliability analysis. By means of the present method, the health condition of an urban river ecosystem can be quickly, accurately and objectively reflected.
Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Detection Center of Microbiology (China)
Inventor
Xie, Xiaobao
Li, Wenru
Zeng, Taohua
Zhang, Zhiqing
Shi, Qingshan
Abstract
An application of geraniol in preparation of formulation for promoting synthesis of Pseudomonas aeruginosa 3OC12-HSL signal molecules is provided. It was found that geraniol slightly inhibits growth of Pseudomonas aeruginosa PAO1 strain, but can significantly promote synthesis of the 3OC12-HSL signal molecules of the bacterium, and thus can be applied to preparation of formulation for promoting synthesis of the Pseudomonas aeruginosa 3OC12-HSL signal molecules.
A01N 49/00 - Biocides, pest repellants or attractants, or plant growth regulators containing compounds containing the group wherein m+n≥1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group wherein A means a carbon atom or Y, n ≥ 0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
9.
METHOD FOR PREPARING SILVER NANOPARTICLES ON BASIS OF AMARANTHUS TRICOLOR L EXTRACT
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Xie, Xiaobao
Zhang, Zhiqing
Li, Wenru
Yao, Junwei
Liu, Dezan
Shi, Qingshan
Abstract
A method for preparing silver nanoparticles on the basis of Amaranthus tricolor L extract. An Amaranthus tricolor L ethanol extract is mixed with a silver ion solution, which react to generate silver nanoparticles. The Amaranthus tricolor L extract is used for reducing silver ions, and silver nanoparticles having a particle size of 1-30 nm can be prepared. Amaranthus tricolor L extract is used to prepare silver nanoparticles, the preparation method is simple and easy to implement, operability is high and costs are low. The Amaranthus tricolor L extract serves as a reducing agent and a stabilizer at the same time, no other chemical components need to be added in the reaction process, and a large number of silver nanoparticles are prepared in a short space of time. The present invention is a simple and also environmentally friendly silver nanoparticle preparation method, and the prepared and obtained silver nanoparticle solution has an efficient antibacterial effect.
B22F 9/24 - Making metallic powder or suspensions thereofApparatus or devices specially adapted therefor using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Xie, Liwei
Abstract
The use of one or more strains of Lactobacillus reuteri PLBK®1, Lactobacillus reuteri PLBK®2, Lactobacillus gasseri PLBK®3, Lactobacillus acidophilus PLBK®4 and Bifidobacterium lactis PLBK®5 and fermentation cultures or metabolites thereof in the preparation of a product for alleviating colorectal inflammation. The use can reduce macrophage infiltration, reduce intestinal inflammation, regulate the microenvironment of intestinal flora and accelerate the repair of intestinal mucosa, and the formula is expected to be used in the clinical prevention and treatment of inflammatory bowel disease associated with colorectal inflammation.
A61K 35/747 - Lactobacilli, e.g. L. acidophilus or L. brevis
A61P 1/16 - Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
GUANGDONG BOWOTE BIOTECHNOLOGY CO., LTD. (China)
Inventor
Zhu, Honghui
Su, Buli
Li, Anzhang
Deng, Mingrong
Feng, Guangda
Abstract
Provided are a mutant strain of carotenoid-producing yeast and an application of a mutation site thereof. Saccharomyces cerevisiae W2, deposited in the GuangDong Microbial Culture Collection Center, having the deposit number GDMCC No: 61335. On the basis of W2, strain W2-A-5 was obtained by further modification, and the deposit number is GDMCC No: 61337. A Saccharomyces cerevisiae mutant strain W2 is obtained by means of microgravity mutagenesis screening. The carotenoid content of the strain can reach 45mg/g cell weight under conditions of shake flask fermentation. On the basis of this strain, W2-A-5 was obtained from further modification. The carotenoid content can reach 68 mg/g dry cell weight. From genome sequencing and reverse metabolic engineering research, it was determined that CHO2 gene inactivation is the main factor for the enhanced carotenoid synthesis ability of W2. After CHO2 inactivation in strains without growth retardation, the ability to synthesize carotenoids was significantly increased, thereby providing a new target for targeted modification of Saccharomyces cerevisiae for increasing carotenoid synthesis ability.
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C12N 15/01 - Preparation of mutants without inserting foreign genetic material thereinScreening processes therefor
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Xie, Liwei
Abstract
Provided are a formula of probiotics and metabolites thereof for alleviating metabolic syndromes, and the use thereof. The formula comprises Lactobacillus reuteri PLBK®1, Lactobacillus reuteri PLBK®2, Lactobacillus gasseri PLBK ®3, Lactobacillus acidophilus PLBK®4 and Bifdobacterium lactis PLBK®5, or fermentation cultures thereof, or metabolites thereof. The use refers to the use of the above-mentioned formula of probiotics in the preparation of a product for alleviating metabolic syndromes, and alleviating the metabolic syndromes is to reduce fat accumulation, promote lipolysis, improve insulin sensitivity and/or alleviate fatty liver.
A61K 35/747 - Lactobacilli, e.g. L. acidophilus or L. brevis
A61P 1/16 - Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
GUANGDONG BOWOTE BIOTECHNOLOGY CO., LTD. (China)
Inventor
Zhu, Honghui
Zhou, Lian
Xie, Xiaolin
Chen, Meibiao
Yao, Qing
Abstract
A method for producing an amino acid liquid fertilizer from waste feathers includes: acquiring an enzymatic hydrolysate by performing enzymolysis on feather powder with a complex enzyme; adding acid protease to the enzymatic hydrolysate for enzymolysis; and acquiring the amino acid liquid fertilizer by performing enzyme inactivation on a filtrate acquired by performing filtering after enzymolysis is completed, wherein the complex enzyme includes keratinase and amino acid peptidase. By adopting this method, the enzymolysis rate of the feathers reaches 80% or above and the prepared amino acid liquid fertilizer contains various kinds of amino acids (17 amino acids); and the content of the amino acid can reach 10.12% (by mass fraction) or above and the content of small peptide reaches 9.39% (by mass fraction), which reach the Chinese standard of the amino acid liquid fertilizers without concentration. Thus, the environmental-friendly amino acid liquid fertilizer can be prepared.
The present invention provides a specific molecular target-containing diarrheagenic Escherichia coli standard reference strain, and a detection and application thereof. The diarrheagenic Escherichia coli (the strain number is PY009-2, 3025B1) of the present invention has the standard microscopic morphology and physiological and biochemical characteristics of Escherichia coli, can be used for testing the characteristics of the Escherichia coli and verifying the accuracy of a color plate, carries a virulence gene, has typical representativeness, has a specific molecular marker, and can be used as a reference strain for genetic evolution research.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
15.
STANDARD STRAINS OF LISTERIA MONOCYTOGENES CONTAINING SPECIFIC MOLECULAR TARGET, AND DETECTION AND APPLICATION THEREOF
The present invention provides a specific molecular target for detecting Listeria monocytogenes, and strains containing same and a quantitative preservation method therefor. The specific molecular target comprises nucleotide sequences shown in SEQ ID NOs: 1-4, and the accession numbers of four strains of Listeria monocytogenes containing the specific molecular target being GDMCC 60836, GDMCC 60838, GDMCC 60839, and GDMCC 60840, respectively. The strains can be used for testing the accuracy of Listeria monocytogenes chromogenic plates and as standard strains. The present invention also provides a freeze-drying protective agent for Listeria monocytogenes.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
16.
CRONOBACTER STANDARD STRAINS CONTAINING SPECIFIC MOLECULAR TARGET, AND DETECTION AND USE THEREOF
Provided are four Cronobacter standard strains, with deposit numbers of GDMCC60861, GDMCC60862, GDMCC60863 and GDMCC60864, respectively. The standard strains contain corresponding specific molecular targets (i.e., nucleotide sequences as shown in SEQ ID NOs: 1- 4). Also provided are primers for detecting the specific molecular targets. Further provided in the present invention is a cryoprotectant for Cronobacter strains.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
The present invention relates to the technical field of bioengineering, and in particular to five standard strains of Bacillus cereus which appear in China Autonomous Intellectual Property and conform to Domestic Propagation Law. In addition, same can be used as the standard reference strains in different fields such as food, medicine, clinical examination and the like. The deposit numbers thereof are GDMCC 60865, GDMCC 60866, GDMCC 60867, GDMCC 60868 and GDMCC 60869, respectively. Five standard strains have typical physiological and biochemical characteristics, have clear information in the sample source, the genetic background, the drug resistance, the virulence gene and the like, and completely meet the requirements of food, medicine and clinical examination on a standard strain. The present invention also relates to a set of specific target genes for detecting and identifying the above-mentioned five standard strains, and the corresponding PCR primers. Finally, also provided in the present invention is a freeze-drying protective agent for Bacillus cereus with a high survival rate and specificity, which can be used for long-term storage of the standard strains of the present invention.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
The present invention provides three strains of Campylobacter jejuni, the preservation number of the strain 346-1C being GDMCC 60857, the preservation number of the strain 3853-1A being GDMCC 60858, and the preservation number of the strain 542-1A being GDMCC 60859. And also provided are specific molecular targets respectively used for examining three specific strains of Campylobacter jejuni, the molecular targets comprising nucleotide sequences as shown in SEQ ID NO: 1-3. Each strain has a standard microscopic morphology and physiological and biochemical properties of Campylobacter jejuni thallus, and can be used for examining the accuracy of a color development plate for Campylobacter jejuni and standard strains examined by related reagents. The present invention also provides a lyoprotectant for Campylobacter jejuni.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
Provided is a specific molecular target for detecting a food-borne pathogenic bacteria. The nucleotide sequence of the specific molecular target is shown in SEQ ID NOs: 1-31. Also provided in the present invention is a detection method for live bacteria of food-borne pathogenic bacteria. The method comprises adding PMA dye solution into a sample to be tested, and then carrying out real-time fluorescence quantitative PCR detection.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Disclosed in the present invention are Staphylococcus aureus standard strains, which respectively have deposit numbers of GDMCC 60841, GDMCC 60843, GDMCC 60844 and GDMCC 60845, and can be used in different fields such as food, medicine and clinical examination. The present invention also relates to a specific target gene for detecting and identifying the aforementioned four Staphylococcus aureus standard strains and a corresponding PCR primer thereof. Further provided in the present invention is a lyoprotectant for Staphylococcus aureus, which can be used for long-term storage of the standard strains of the present invention.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Disclosed are standard reference strains of staphylococcus argenteus with preservation numbers GDMCC 60854, GDMCC 60948 and GDMCC 60949, respectively, which can be used in different fields such as food, medicament, clinical tests and the like. The present invention further relates to a set of specific target genes and corresponding PCR primers for the detection, identification of the above 3 standard strains of staphylococcus argenteus. The present invention further provides a lyoprotectant of staphylococcus argenteus, which can be used for long-term storage of the standard strains in the present invention.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Provided in the present invention is a specific molecular target for detecting five Salmonella strains. The molecular target is: (a) any one or several nucleotide sequences as shown in SEQ ID NOs: 1-5; or (b) a nucleotide sequence obtained by substituting, deleting or adding of one or several nucleotides of the nucleotide sequence in (a) and having at least 90% homology thereto. Further provided in the present invention are five Salmonella standard strains containing the specific molecular target, which respectively have deposit numbers of GDMCC 60847, GDMCC 60848, GDMCC 60849 and GDMCC 60850.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
23.
VIBRIO PARAHAEMOLYTICUS STANDARD STRAINS CONTAINING SPECIFIC MOLECULAR TARGET, AND DETECTION AND USE THEREOF
The present invention relates to the technical field of bioengineering, and in particular relates to three Vibrio parahaemolyticus standard strains which conform to domestic propagation laws and can be used as standard reference strains in different fields, such as food, medicines, and clinical examination. The deposit numbers thereof are respectively GDMCC 60870, GDMCC 60871 and GDMCC 60872. The three standard strains have typical physiological and biochemical characteristics, contain clear information such as sample sources, genetic backgrounds, drug resistance and virulence genes, and completely meet the requirements for standard strains in the aspects of food, medicines, clinical examinations, etc. The present invention further relates to a group of specific target genes for detecting and identifying the three above-mentioned standard strains and corresponding PCR products. Finally, further provided is a freeze-drying protective agent of Vibrio parahaemolyticus with a high survival rate and specificity, which can be used for long-term storage of the standard strains.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
Yersiniaenterocoliticaenterocolitica related experiments. The standard strains contain a corresponding specific molecular target (such as a nucleotide sequence as shown in SEQ ID NO: 1-11). Also provided is a primer for detecting the specific molecular target. Further provided in the present invention is a freeze-drying protective agent of Yersinia enterocolitica.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Xie, Xiaobao
Yang, Ping
Shi, Qingshan
Huang, Xiaomo
Shu, Xiulin
Abstract
Disclosed in the present invention are a guanidinothiazole compound, and a preparation method therefor and the use thereof. The structural formula of the guanidinothiazole compound is as shown in formula (IV). According to the present invention, a series of guanidinothiazole compounds of new structures are designed and synthesized by means of introducing a lipophilic side chain into the C2 position and hydrophilic guanidyl into the C5 position of a thiazole ring. The preparation method therefor comprises: performing a condensation reaction on an aldehyde compound (I) and thiosemicarbazide, which serve as as raw materials, to obtain a first-step product (II); reacting the first-step product (II) with 3-chloroacetylacetone to obtain a second-step product (III); and reacting the second-step product (III) with aminoguanidine hydrochloride to prepare a final product (IV). The guanidinothiazole compound exhibits an antibacterial activity, and especially has a good antibacterial activity on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, which compound can be used as an antibacterial candidate compound.
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
INFINITUS (CHINA) COMPANY LTD. (China)
Inventor
Wu, Qingping
Tang, Jian
Kou, Xiuying
Hu, Huiping
Liang, Xiaowei
Xie, Yizhen
Liu, Yuanchao
Cai, Manjun
Xiao, Chun
Li, Xiangmin
Abstract
Disclosed are a low-spore variety of Ganoderma lucidum having a high polysaccharide yield and an artificial cultivation method therefor. Ganoderma lucidum M624, having an accession number of: GDMCC No: 60889. An artificial cultivation method, comprising mycelium culture, fungus growth management, and fruiting body harvesting of Ganoderma lucidum M624. With the present low-spore variety of Ganoderma lucidum having high polysaccharide yield — Ganoderma lucidum M624, in substitute cultivation, the content of crude polysaccharide in the fruiting body reaches 2.40±0.09%. Additionally, the Ganoderma lucidum M624 produces few spores, thus being able to reduce the impact thereof on the cultivation environment and machinery, and satisfy Ganoderma lucidum cultivation in which the fruiting body is the harvesting target.
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
A01N 49/00 - Biocides, pest repellants or attractants, or plant growth regulators containing compounds containing the group wherein m+n≥1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group wherein A means a carbon atom or Y, n ≥ 0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
A01P 1/00 - DisinfectantsAntimicrobial compounds or mixtures thereof
A61K 31/045 - Hydroxy compounds, e.g. alcoholsSalts thereof, e.g. alcoholates
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
GUANGDONG PROBIOCARE BIOTECHNOLOGY CO LTD (China)
Inventor
Xie, Liwei
Liu, Bingdong
Han, Mulan
Abstract
Provided is a use of a ferroptosis inhibitor in preparing a preparation for improving the motor function of a senescent individual. Transcriptome sequencing analysis has shown that TfR1 expression in skeletal muscle and muscle satellite cells decreases with increasing age. Muscle satellite cell-specific knockout of TfR1 results in irreversible inactivation of muscle satellite cells. The skeletal muscle regeneration process in TfR1 muscle satellite cell-specific mice is accompanied by muscle atrophy, iron ion accumulation and increased unsaturated fatty acid biosynthesis, thereby inducing skeletal muscle ferroptosis. Senescent skeletal muscle exhibits a decrease in TFR1 expression, while membrane surface accumulation of Slc39a14 promotes increased absorption of iron via binding with non-iron transport proteins, promoting the occurrence of ferroptosis. Intraperitoneal injection of ferrostatin-1 in senescent mice can significantly ameliorate muscle regeneration difficulties and motor difficulties caused by skeletal muscle death.
A61K 31/245 - Amino benzoic acid types, e.g. procaine, novocaine
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
A61P 21/00 - Drugs for disorders of the muscular or neuromuscular system
A61K 31/138 - Aryloxyalkylamines, e.g. propranolol, tamoxifen, phenoxybenzamine
29.
SELENIUM-RICH CORDYCEPS MILITARIS DERIVED ACTIVE SELENIUM PEPTIDE WITH NEURON PROTECTION FUNCTION, AND PREPARATION METHOD THEREFOR AND USE THEREOF
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Li, Wenru
Xie, Xiaobao
Zeng, Taohua
Zhang, Zhiqing
Liu, Jingxia
Shi, Qingshan
Abstract
Provided is the use of citronellol in the preparation of a preparation for promoting the expression of a virulence gene toxA from Pseudomonas aeruginosa. Citronellol can promote the transcription of toxA from Pseudomonas aeruginosa, and as such, the yield of a coding product of toxA, namely, exotoxin A, can be increased.
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Ding, Yu
Zhang, Junhui
Zhou, Huan
Wu, Qingping
Wang, Juan
Zhu, Zhenjun
Zhang, Jumei
Ye, Qinghua
Chen, Moutong
Xue, Liang
Wu, Shi
Zeng, Haiyan
Pang, Rui
Zhang, Shuhong
Yang, Xiaojuan
Abstract
Provided is a nucleic acid detection method for rapidly identifying Bacillus cereus and Bacillus thuringiensis. The method comprises selecting different SNP sites in a molecular target ispD gene sequence to design primers, and respectively carrying out an HRM experiment by means of taking Bacillus cereus and Bacillus thuringiensis as templates to acquire the characteristic melting curve information of corresponding strains, thereby completing the accurate identification of Bacillus cereus and Bacillus thuringiensis.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Wu, Qingping
Chen, Minling
Ding, Yu
Wang, Juan
Zhang, Jumei
Zhang, Junhui
Zhou, Huan
Yu, Shubo
Chen, Moutong
Xue, Liang
Ye, Qinghua
Wei, Xianhu
Zhang, Youxiong
Abstract
A rapid identification method of Bacillus cereus and Bacillus thuringiensis based on MALDI-TOF MS. The method uses a 50S ribosomal protein L30, a 50S ribosomal protein L31 Type B, and a 30S ribosomal protein S20 as characteristic proteins. Screening and identification of signature peaks by MALDI-TOF MS are guided by whole genome sequencing information of Bacillus cereus and Bacillus thuringiensis to obtain by MALDI-TOF MS the signature peaks that can accurately differentiate Bacillus cereus from Bacillus thuringiensis. The results are accurate and reliable so as to provide new thoughts about applying MALDI-TOF MS to differentiate closely related species.
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Zhu, Honghui
Su, Buli
Wang, Dongdong
Li, Anzhang
Deng, Mingrong
Feng, Guangda
Abstract
Provided is a fermentation culture medium beneficial for the synthesis of Saccharomyces cerevisiae carotenoid, the medium comprising a basic culture medium for Saccharomyces cerevisiae, and copper ions. The fermentation culture medium is used to improve the accumulation of recombinant Saccharomyces cerevisiae carotenoid. A synergistic enhancement effect present between zinc ions and the copper ions in the culture medium is shown from a transcriptome, and the synthesis of carotenoid can be significantly improved by means of adding the two.
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
34.
SACCHAROMYCES CEREVISIAE REGULATORY ELEMENT AND USE THEREOF IN CAROTENOID SYNTHESIS
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Zhu, Honghui
Su, Buli
Wang, Dongdong
Li, Anzhang
Deng, Mingrong
Feng, Guangda
Abstract
Provided are a regulatory element having a nucleotide sequence as shown in SEQ ID NO:1 that antagonizes the genomic position effect of Saccharomyces cerevisiae, and the use thereof. Chromosomes near the gal80 site of Saccharomyces cerevisiae have special structures, and may have other types of position effects that are non-HM site (mating type allocation site) silencing effects. The transcription activation region of TEF2 is added to two ends of a synthetic pathway with a gene sequence of crtE-crtB-crtI, which can antagonize the unknown position effect of the gal80 site and significantly increase the content of lycopene. The antagonistic effect mainly prevents the influence of the position effect on gene transcription in the synthetic pathway.
INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) (China)
Inventor
Zhu, Honghui
Su, Buli
Feng, Guangda
Deng, Mingrong
Li, Anzhang
Abstract
A saccharomyces mutant strain for producing carotenoid and use of mutation sites thereof. Saccharomyces cerevisiae M3 is deposited in Guangdong Microbial Culture Collection Center, and has GDMCC Deposit No: 61336. A saccharomyces cerevisiae mutant strain M3 is obtained by screening by means of ethanol-mediated adaptive laboratory evolution. Under a condition of shake flask fermentation, the content of carotenoid of the strain in a YPD culture medium can reach 19.7 mg/g, which is improved by 5.1 times compared with that of an original strain; and the content of carotenoid of the strain in a YPM culture medium can reach 42.4 mg/g of dry cell weight. By means of genome sequencing and reverse metabolic engineering research, it is determined that PFK1 gene inactivation is a main factor for carotenoid accumulation improvement, and a new target is provided for directional transformation.
C12N 15/01 - Preparation of mutants without inserting foreign genetic material thereinScreening processes therefor
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
patents
