Abstract

The method comprises a step of culture of genetically engineered hairy roots in a culture medium, a step of elicitation of the production of a secondary metabolite of interest; optionally a step of induction of the secretion of the secondary metabolite into the culture medium by addition of an agent, and a step of collection of the culture medium containing the secreted metabolite of interest with addition of fresh culture medium, wherein the steps of secretion and collection are repeated sequentially.

IPC Classes  ?

• A01H 4/00 - Plant reproduction by tissue culture techniques

Abstract

A bioreactor for the production of biomass configured to contain at least 15 liters, with an external support structure displaying a general annular shape, an internal tank being configured to be secured inside and supported by the external support structure. The internal space of the internal tank is free of any internal structure. The bioreactor includes a sparger located at a closed extremity of the internal tank. The sparger is configured to generate bubbles displaying a diameter of at least 5 millimeters. The sparger and the internal tank are arranged in a way that the generated bubbles form one bubble column once the internal tank is filled with liquid and the sparger is fed with gas, the bubble column enabling the liquid to be put in motion.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means

Abstract

A method for estimating the hairy root biomass concentration X(t) of a multi-phasic culture of hairy roots in a culture medium, the culture medium including at least one source of carbon being at least one sugar, and at least one source of nitrogen. The biomass yield surprisingly correlates linearly with the consumed concentration of nitrogen and/or sugars over the entire time course of the multi-phasic culture of hairy roots. The method for estimating the hairy root biomass concentration X(t) according to the invention is also a useful tool to estimate the amount of one or more molecule(s) of interest synthesized by the hairy roots.

IPC Classes  ?

• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
• A01G 7/00 - Botany in general
• A01H 4/00 - Plant reproduction by tissue culture techniques

Abstract

A method for recovering a secreted compound of interest in a hairy roots-based expression system by contacting the hairy roots with a solution including from about 250 mM to about 4 M of a salt. This method allows to increase the yield of recovery of the secreted compound of interest.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)

Abstract

The present invention relates to a bioreactor (10) for the production of biomass configured to contain at least 15 liters, with an external support structure (12) displaying a general annular shape, an internal tank (14) being configured to be secured inside and supported by the external support structure. The internal space (22) of the internal tank is free of any internal structure. The bioreactor comprises a sparger (16) located at a closed extremity (20) of the internal tank. The sparger is configured to generate bubbles (26) displaying a diameter of at least 5 millimeters. The sparger and the internal tank are arranged in a way that the generated bubbles form one bubble column once the internal tank is filled with liquid and the sparger is fed with gas, said bubble column enabling the liquid to be put in motion.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• B01F 33/40 - Mixers using gas or liquid agitation, e.g. with air supply tubes
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Abstract

The present invention relates to a bioreactor (10) for the production of biomass configured to contain at least 15 liters, with an external support structure (12) displaying a general annular shape, an internal tank (14) being configured to be secured inside and supported by the external support structure. The internal space (22) of the internal tank is free of any internal structure. The bioreactor comprises a sparger (16) located at a closed extremity (20) of the internal tank. The sparger is configured to generate bubbles (26) displaying a diameter of at least 5 millimeters. The sparger and the internal tank are arranged in a way that the generated bubbles form one bubble column once the internal tank is filled with liquid and the sparger is fed with gas, said bubble column enabling the liquid to be put in motion.

IPC Classes  ?

• B01F 33/40 - Mixers using gas or liquid agitation, e.g. with air supply tubes
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Abstract

The present invention relates to a method for estimating the hairy root biomass concentration X(t) of a multi-phasic culture of hairy roots in a culture medium, the culture medium comprising at least one source of carbon being at least one sugar, and at least one source of nitrogen. The inventors found that the biomass yield surprisingly correlates linearly with the consumed concentration of nitrogen and/or sugars over the entire time course of the multi-phasic culture of hairy roots. The method for estimating the hairy root biomass concentration X(t) according to the invention is also a useful tool to estimate the amount of one or more molecule(s) of interest synthesized by the hairy roots.

IPC Classes  ?

• A01G 24/00 - Growth substratesCulture mediaApparatus or methods therefor

Abstract

The present invention relates to a method for estimating the hairy root biomass concentration X(t) of a multi-phasic culture of hairy roots in a culture medium, the culture medium comprising at least one source of carbon being at least one sugar, and at least one source of nitrogen. The inventors found that the biomass yield surprisingly correlates linearly with the consumed concentration of nitrogen and/or sugars over the entire time course of the multi-phasic culture of hairy roots. The method for estimating the hairy root biomass concentration X(t) according to the invention is also a useful tool to estimate the amount of one or more molecule(s) of interest synthesized by the hairy roots.

IPC Classes  ?

• A01G 24/00 - Growth substratesCulture mediaApparatus or methods therefor

Abstract

The invention relates to a method for recovering a secreted compound of interest in a hairy roots-based expression system by contacting the hairy roots with a solution comprising from about 250 mM to about 4 M of a salt. This method allows to increase the yield of recovery of said secreted compound of interest.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

The invention relates to a method for recovering a secreted compound of interest in a hairy roots-based expression system by contacting the hairy roots with a solution comprising from about 250 mM to about 4 M of a salt. This method allows to increase the yield of recovery of said secreted compound of interest.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells