Abstract

Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.

IPC Classes  ?

• A61D 19/02 - Instruments or methods for reproduction or fertilisation for artificial insemination
• A01K 67/02 - Breeding vertebrates
• C12N 5/076 - Sperm cellsSpermatogonia
• A01N 1/02 - Preservation of living parts

Abstract

Provided is a diluent used for preserving highly-fertile sperm. The diluent is cheaper than that of the prior art and enables the quality of sperm to be improved. In addition, stable quality control of the diluent is possible. Further provided is a method for preserving sperm using the diluent.　As a result of refrigerating or freezing sperm for the purpose of preservation using this diluent for sperm, which is characterized by being an aqueous solution containing oligosaccharides, including at least one of fructooligosaccharides, isomaltooligosaccharides, gentiooligosaccharides, and galactooligosaccharides, it is possible to reduce costs while improving the quality of sperm after preservation, and it is possible to provide highly-fertile sperm.

IPC Classes  ?

• C12N 5/076 - Sperm cellsSpermatogonia
• A01N 1/02 - Preservation of living parts
• A61D 19/02 - Instruments or methods for reproduction or fertilisation for artificial insemination
• A01K 67/02 - Breeding vertebrates

Abstract

The present invention addresses the problem of developing a simple sperm inspection method and device for reflecting fertility. In the present invention, a simple sperm inspection method and device having a high correlation with fertility can be provided by suspending a sperm population in an inspection liquid and measuring a quality indicator value for each sperm, stratifying the sperm population on the basis of the sperm quality indicator value, and evaluating the quality of the sperm on the basis of the quality indicator value of one or a plurality of groups in the stratified population. This standard can predict fertility with higher precision through use of a graph in which the quality indicator values for individual sperm of the sperm population are arranged in sequence. Sperm motility is preferably used as a sperm quality indicator.

IPC Classes  ?

• G06T 7/00 - Image analysis
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

　The present invention addresses the problem of developing a simple sperm inspection method and device for reflecting conception chance. In the present invention, a simple sperm inspection method and device having a high correlation with conception chance can be provided by suspending a sperm population in an inspection liquid and measuring a quality indicator value for each sperm, stratifying the sperm population on the basis of the sperm quality indicator value, and evaluating the quality of the sperm on the basis of the quality indicator value of one or a plurality of groups in the stratified population. This standard can predict conception chance with higher precision through use of a graph in which the quality indicator values for individual sperm of the sperm population are arranged in sequence. Sperm motility is preferably used as a sperm quality indicator.

IPC Classes  ?

• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• G01N 33/483 - Physical analysis of biological material

Abstract

[Problem] To improve the conception chance of semen after cryopreservation by improving conventional straws for artificial insemination. [Solution] A straw for cryopreservation is produced by introducing a partitioning layer into a straw for artificial insemination to separate layers, thereby forming a semen storage layer comprising an aqueous solution containing semen and an anti-freeze agent on one side and a dilution layer containing no anti-freeze agent on the other side, wherein a dilution solution comprising an aqueous solution containing tris(hydroxymethyl aminomethane), citric acid, glucose and sodium chloride is used in the dilution layer.

IPC Classes  ?

• A01K 67/02 - Breeding vertebrates

Abstract

Provided is a diluent used for preserving highly-fertile sperm. The diluent is cheaper than that of the prior art and enables the quality of sperm to be improved. In addition, stable quality control of the diluent is possible. Further provided is a method for preserving sperm using the diluent. As a result of refrigerating or freezing sperm for the purpose of preservation using this diluent for sperm, which is characterized by being an aqueous solution containing oligosaccharides, including at least one of fructooligosaccharides, isomaltooligosaccharides, gentiooligosaccharides, and galactooligosaccharides, it is possible to reduce costs while improving the quality of sperm after preservation, and it is possible to provide highly-fertile sperm.

IPC Classes  ?

• A01K 67/02 - Breeding vertebrates
• A61D 19/02 - Instruments or methods for reproduction or fertilisation for artificial insemination
• C12N 5/076 - Sperm cellsSpermatogonia