Abstract

Provided herein are genetically modified yeast cells that recombinantly express a gene encoding an acyl-activating enzyme (AAE) enzyme or overexpress an AAE enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/10 - Transferases (2.)
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 1/18 - Baker's yeastBrewer's yeast
• C12G 1/02 - Preparation of must from grapesMust treatment or fermentation
• C12P 7/06 - Ethanol, i.e. non-beverage
• C12P 7/52 - Propionic acidButyric acids
• C12P 7/62 - Carboxylic acid esters
• C12P 7/6436 - Fatty acid esters
• C12C 11/00 - Fermentation processes for beer
• C12C 12/00 - Processes specially adapted for making special kinds of beer

Abstract

Provided herein are genetically modified yeast cells that recombinantly expresses a gene encoding a mutant beta-lyase. Also provided are methods of producing fermented products and methods of producing ethanol.

IPC Classes  ?

• C12C 12/00 - Processes specially adapted for making special kinds of beer
• C12C 11/00 - Fermentation processes for beer
• C12G 1/022 - FermentationMicrobiological or enzymatic treatment
• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/88 - Lyases (4.)
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Abstract

e.g.e.g., non-alcoholic fermented beverages) using the genetically modified yeast cells described herein.

IPC Classes  ?

• C07K 14/395 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi from yeasts from Saccharomyces
• C12C 12/00 - Processes specially adapted for making special kinds of beer
• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/10 - Transferases (2.)
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 9/88 - Lyases (4.)
• C12C 12/04 - Beer with low alcohol content

Abstract

Provided herein are genetically modified yeast cells that recombinantly express a glycosidase enzyme. Provided herein are genetically modified yeast cells that recombinantly express a glycosidase enzyme and/or an O-methyltransferase enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12G 1/022 - FermentationMicrobiological or enzymatic treatment
• C12H 1/00 - Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
• C12H 1/044 - Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material with the aid of inorganic material
• C12H 1/056 - Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material with the aid of organic material with the aid of polymers
• C12H 1/07 - Separation by filtration
• C12N 9/10 - Transferases (2.)
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase

Abstract

Provided herein are genetically modified yeast cells that recombinantly express a gene encoding a fatty acid hydroxylase (FAH) enzyme, such as an oleate 12-hydroylase, and produce y-decalactone levels above an odor-threshold. Also provided herein are genetically modified yeast cells that recombinantly express genes encoding a fatty acid hydroxylase (FAH) enzyme, and one or more additional genes, such as an acyl-CoA desaturase 1 (OLE1) enzyme, a deregulated transcription factor, and/or an alcohol-O-acyltransferase (AAT) enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12C 12/00 - Processes specially adapted for making special kinds of beer
• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12P 7/06 - Ethanol, i.e. non-beverage
• C12P 17/04 - Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin

Abstract

Provided herein are genetically modified yeast cells that recombinantly express a gene encoding an alcohol-O-acyltransferase (AAT) enzyme and a gene encoding a fatty acid synthase a subunit (FAS2) enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12C 12/00 - Processes specially adapted for making special kinds of beer
• C12G 1/022 - FermentationMicrobiological or enzymatic treatment

Abstract

Provided herein are genetically modified yeast cells that recombinantly express a glycosidase enzyme. Provided herein are genetically modified yeast cells that recombinantly express a glycosidase enzyme and/or an O-methyltransferase enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12C 11/11 - Post fermentation treatments, e.g. carbonation or concentration
• C12G 1/022 - FermentationMicrobiological or enzymatic treatment
• C12G 1/06 - Preparation of sparkling wineImpregnation of wine with carbon dioxide
• C12G 3/08 - Preparation of other alcoholic beverages by methods for altering the composition of fermented solutions or alcoholic beverages not provided for in groups
• A23L 2/84 - Clarifying or fining of non-alcoholic beveragesRemoving unwanted matter using microorganisms or biological material, e.g. enzymes
• A62D 3/02 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
• C12N 9/10 - Transferases (2.)
• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)

Abstract

Provided herein are genetically modified yeast cells that recombinantly express a gene encoding a fatty acid hydroxylase (FAH) enzyme, such as an oleate 12-hydroylase, and produce y-decalactone levels above an odor-threshold. Also provided herein are genetically modified yeast cells that recombinantly express genes encoding a fatty acid hydroxylase (FAH) enzyme, and one or more additional genes, such as an acyl-CoA desaturase 1 (OLE1) enzyme, a deregulated transcription factor, and/or an alcohol-O-acyltransferase (AAT) enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12C 12/00 - Processes specially adapted for making special kinds of beer
• C12G 3/02 - Preparation of other alcoholic beverages by fermentation
• C12G 3/024 - Preparation of other alcoholic beverages by fermentation of fruits other than botanical genus Vitis
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 15/53 - Oxidoreductases (1)
• C12N 15/54 - Transferases (2)
• C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
• C12P 17/04 - Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin

Abstract

Provided herein are genetically modified yeast cells that recombinantly express a gene encoding a fatty acid hydroxylase (FAH) enzyme, such as an oleate 12-hydroylase, and produce y-decalactone levels above an odor-threshold. Also provided herein are genetically modified yeast cells that recombinantly express genes encoding a fatty acid hydroxylase (FAH) enzyme, and one or more additional genes, such as an acyl-CoA desaturase 1 (OLE1) enzyme, a deregulated transcription factor, and/or an alcohol-O-acyltransferase (AAT) enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12C 12/00 - Processes specially adapted for making special kinds of beer

Abstract

Provided herein are genetically modified yeast cells that recombinantly express a gene encoding an alcohol-O-acyltransferase (AAT) enzyme and a gene encoding a fatty acid synthase a subunit (FAS2) enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.

IPC Classes  ?

• C12C 11/00 - Fermentation processes for beer
• C12G 1/00 - Preparation of wine or sparkling wine
• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/10 - Transferases (2.)

Abstract

Provided herein are genetically modified yeast cells that recombinantly expresses a gene encoding a mutant beta-lyase. Also provided are methods of producing fermented products and methods of producing ethanol.

IPC Classes  ?

• C07K 14/32 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Bacillus (G)
• C12P 13/04 - Alpha- or beta-amino acids
• C12P 13/12 - MethionineCysteineCystine