2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.
C12P 7/64 - Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
C12P 39/00 - Processes involving microorganisms of different genera in the same process, simultaneously
Apparatuses and associated methods are described for the efficient evaluation of C1-containing substrates, and especially for such evaluation conducted locally, or on-site, at a prospective facility for implementation of a biological conversion process for desired end product using a C1 carbon source. The exact composition of a given, industrial C1-containing substrate, as well as the range in composition fluctuations, are generally difficult to reproduce at a remote facility (e.g., a laboratory or a pilot-scale or demonstration-scale process), as required for the accurate prediction/modeling of commercial performance to justify large capital expenditures for commercial scale-up.
G01N 30/46 - Flow patterns using more than one column
G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.
The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.
Improvements in biological conversion processes and associated apparatuses are disclosed for the generation of useful end products such as ethanol, through metabolic pathways of C1-fixing bacteria that utilize, as a nutrient, a C1-carbon source from a C1-containing substrate such as an industrial waste gas. Particular aspects of the disclosure relate to the downstream recovery of ethanol and/or isopropanol from bleed and permeate streams and more particularly to performing such recovery with improved efficiency that can advantageously reduce capital (e.g., equipment) and/or operating (e.g., utility) costs. Particular aspects related to the downstream recovery of ethanol and/or isopropanol using a low pressure separator having an internal divider.
Improvements in biological conversion processes and associated apparatuses are disclosed for the generation of useful end products such as ethanol, through metabolic pathways of C1-fixing bacteria that utilize, as a nutrient, a C1-carbon source from a C1-containing substrate such as an industrial waste gas. Particular aspects of the disclosure relate to the downstream recovery of ethanol and/or isopropanol from bleed and permeate streams and more particularly to performing such recovery with improved efficiency that can advantageously reduce capital (e.g., equipment) and/or operating (e.g., utility) costs.
2 generated by the electrolysis process is used to improve the composition of the C1-containing tail gas generated by the C1-generating industrial process.
The invention provides methods for improving efficiency of fermentation by arginine supplementation, and genetically modified bacterium for use therefor. More particularly the invention provides methods for (i) increasing the production ATP intensive products with arginine supplementation, (ii) increasing utilization of arginine by a C1-fixing bacterium; and (iii) providing C1-fixing bacterium with optimized arginine de-aminase pathways.
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.
The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation.
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymes; General processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
Clostridium bacterium that expresses one or more of pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1), acetolactate synthase (EC 2.2.1.6), and acetolactate decarboxylase (EC 4.1.1.5). The invention further provides a method of producing a fermentation product by fermenting the recombinant bacterium in the presence of a gaseous substrate comprising CO to produce one or more of ethanol, butanol, isopropanol, isobutanol, higher alcohols, butanediol, 2,3-butanediol, succinate, isoprenoids, fatty acids, biopolymers, and mixtures thereof.
2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.
C12P 7/64 - Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
C12P 39/00 - Processes involving microorganisms of different genera in the same process, simultaneously
Multi-stage, biological processes and systems for converting a C1 carbon source to desired end products are described. The processes comprise dividing a gaseous C1-containing substrate, in parallel, among multiple bioreactor stages. Liquid products are successively fed, in series, from a first bioreactor stage to downstream bioreactor stages. Operation can be simplified by avoiding the requirement for microorganism separation and recycle at each stage. In addition, overall vapor-liquid mass transfer for the combined stages is very favorable, leading to high end product productivity with comparably low byproduct metabolite productivity.
The invention provides genetically engineered microorganisms with altered carbon monoxide dehydrogenase (CODH) activity and methods related thereto. In particular, the invention provides a genetically engineered carboxydotrophic acetogenic bacterium having decreased or eliminated activity of CODH1 and/or CODH2. In certain embodiments, the bacterium may also have increased activity of CODH/ACS. The invention further provides a method for producing a product by culturing the bacterium in the presence of a gaseous substrate comprising one or more of carbon monoxide, carbon dioxide, and hydrogen.
Processes, as well as associated systems and computer program (software) products, are disclosed for the biological conversion of CO into desired end products such as ethanol. The control methodologies used for these processes can advantageously result in a reduced time required for a batch operation or other initial operating period, prior to achieving a continuous operation, which may be demarcated either by the addition of fresh culture medium at a defined flow rate or by another process initiation target. The control methodologies may alternatively, or in combination, improve a process performance parameter, such as productivity of the desired end product or bacterial growth rate, during this batch operation or other initial operating period.
A process for producing and controlling pyruvate derived products during the fermentation of a CO containing substrate by an acetogenic carboxydotrophic microorganism has been developed. The process involves increasing the concentration of at least one nutrient selected from the group consisting of vitamin B1, vitamin B5, vitamin B7 and mixtures thereof above the cellular requirement of the microorganism. When the concentration is increased, the production of 2,3-butanediol (2,3-BDO) increases whereas the production of the other metabolites is virtually unchanged. The effect is reversible so that when the concentration is decreased, the production of 2,3-BDO is also decreased. This allows one to control the ratio of ethanol:2,3-BDO to a desired value which can vary from about 4:1 to about 1:2.
This invention relates generally to method for producing products, particularly alcohols, by microbial fermentation. In particular, the invention relates to methods for increasing the efficiency of the fermentation, by providing a method for treating the used fermentation broth to produce a treated permeate which is then passed back to the bioreactor. The invention provides a method whereby at least one treatment step used to treat the permeated, produces a gaseous product which is then used in one or more stages of the fermentation process.
The invention provides, inter alia, methods for the production of acetone, isopropanol and/or precursors of acetone and/or isopropanol by microbial fermentation of substrates comprising CO, genetically modified microorganisms of use in such methods, nucleic acids suitable for preparation of genetically modified microorganisms, a novel alcohol dehydrogenase and nucleic acids encoding same.
C12P 7/04 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
C12N 15/00 - Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N 15/52 - Genes encoding for enzymes or proenzymes
The invention provides a genetic tool to insert, replace, delete, or otherwise manipulate a nucleic acid sequence in a microorganism to produce a recombinant microorganism. Notably, the invention makes use of homologous recombination, a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. Since the invention involves three homologous recombination events, it is referred to as a “triple cross” method.
C12N 15/66 - General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C12N 15/90 - Stable introduction of foreign DNA into chromosome
A methods for altering the metabolite profile of a fermentation, by increasing flux through acetolactate. The methods comprises increasing production of one or more products derived from acetolactate. Further provided is a method for increasing the production of 2,3-butandiol by microbial fermentation of gaseous substrates, the method comprising providing a compound which inhibits one or more enzymes which convert acetolactate to branched chain amino acids to the fermentation. The present invention further provides methods for increasing the production of 2,3-butandiol relative to other fermentation products such as ethanol and acetic acid.
A bioreactor system is provided for continuous fermentation of a gaseous substrate, said system comprising two or more primary bioreactors and one or more secondary bioreactors connected by a central bleed line. Further provided is a process for inoculating multiple bioreactors utilizing a central bleed line, said process comprising passing fermentation broth from a first primary bioreactor to other primary bioreactors and/or secondary bioreactors via a central bleed line. Further provided is a process for maintaining stable fermentation of a gaseous substrate across multiple bioreactors, said process comprising providing fermentation broth from one or more operational primary bioreactors to one or more secondary bioreactors via a central bleed line.
A reactor system is provided for improved fermentation of a gaseous substrate through the introduction of a secondary loop to a forced-circulation loop reactor. The reactor comprises a primary loop through which fermentation broth comprising a gaseous substrate is circulated through a riser segment and a downcomer section by a loop pump. Downstream of the loop pump a portion of fermentation broth is withdrawn from the downcomer section and is directed to the top of the reactor via a secondary loop. Further provided is a method for improving the mass transfer of a gaseous substrate to a fermentation broth in a fermentation vessel comprising a secondary loop. Further provided is a method for reducing foam in the headspace of a fermentation vessel comprising a secondary loop.
Processes, as well as associated systems, are disclosed for the biological conversion of CO into desired end products such as ethanol. The use of a plurality of perforated plates, for example in the riser section of a bioreactor, which are positioned substantially horizontally and normal to the upward flow of both a CO-containing substrate and liquid culture medium, can significantly improve CO utilization of the bacteria and consequently the overall process economics. The geometry of apertures in the perforated plates is an important determinant of their performance, with fractal patterns and other multi-edged shapes leading to particularly advantageous results.
The invention provides a recombinant carboxydotrophic Clostridia microorganism with increased overall utilization of NADPH relative to a parent microorganism. Further, the invention provides a method of producing a recombinant carboxydotrophic Clostridia microorganism which exhibits increased NADPH utilization relative to a parental microorganism. In particular, the invention relates to increasing the overall utilization of NADPH in a recombinant carboxydotrophic Clostridia microorganism in order to increase the production of at least one fermentation product by the microorganism.
The invention provides a microbubble generation system with increased efficiency and flexibility compared to known systems. Further, the invention provides a method of microbubble generation. In particular, invention relates to increasing the efficiency of a fermentation reaction by reducing bubble size and increasing gas absorption into a liquid fermentation broth.
B28C 5/06 - Apparatus or methods for producing mixtures of cement with other substances, e.g. slurries, mortars, porous or fibrous compositions without using driven mechanical means effecting the mixing the mixing being effected by the action of a fluid
B01F 3/04 - Mixing, e.g. dispersing, emulsifying, according to the phases to be mixed gases or vapours with liquids
B01F 13/10 - Mixing plant, including combinations of dissimilar mixers
C12M 1/00 - Apparatus for enzymology or microbiology
32.
Recombinant microorganisms and methods of use thereof
The invention relates to methods for the production of chemical compounds, particularly but not exclusively ethanol, by microbial fermentation. Also described are genetically modified micro-organisms capable of using carbon monoxide to produce one or more products, particularly but not exclusively ethanol as a main product, and producing a reduced amount or substantially no 2,3-butanediol and/or a precursor thereof.
C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N 15/00 - Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C12N 15/52 - Genes encoding for enzymes or proenzymes
C12P 7/40 - Preparation of oxygen-containing organic compounds containing a carboxyl group
The invention relates, inter alia, to novel genetically modified microorganisms capable of using CO to produce 1-butanol and/or a precursor thereof, novel methyltransferases and nucleic acids encoding same, methods for producing genetically modified microorganisms using said novel methyltransferases, and methods of producing 1-butanol and/or a precursor thereof by microbial fermentation.
2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce a product comprising acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.
C12P 7/64 - Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
C. autoethanogenum permits the conversion of racemic propanediol to acetone and/or propionaldehyde. Entantiomeric starting materials lead to different products. If desired, the products may be reduced to form alcohols. The reaction can be performed in various host cells, so that various materials may be used as carbon and/or energy sources.
A carboxydotrophic acetogenic recombinant microorganism is modified so that it produces biodiesel and optionally one or more other products by fermentation of a substrate comprising CO. Biodiesel is produced by microbial fermentation of a substrate comprising CO. The recombinant microorganism is modified to express one or more exogenous enzymes in the biodiesel biosynthesis pathway not present in a parental microorganism from which the recombinant microorganism is derived. The one or more enzymes comprise a nonspecific acyltransferase.
C12N 1/21 - Bacteria; Culture media therefor modified by introduction of foreign genetic material
C12P 7/64 - Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
2. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.
The invention provides an improved method for the production, separation and recovery of one or more fermentation products from a fermentation broth. Further, the invention provides a method for increasing efficiency of a fermentation reaction. In particular, the invention relates to a fermentation system which incorporates a simulated moving bed for separation of fermentation products from a fermentation broth, and a corresponding method.
The invention relates to the production of products such as alcohols and acids by microbial fermentation, particularly microbial fermentation of substrates comprising CO. It more particularly relates to methods and systems for improving efficiency of products by microbial fermentation. In particular embodiments, the invention provides a method of optimizing production of desired products including the step of ascertaining the proportion of CO converted to CO2.
The present invention relates to mutant alcohol dehydrogenase enzymes, microorganisms comprising the mutant alcohol dehydrogenase enzymes, and methods for the production of products by microbial fermentation using the microorganisms.
This invention relates generally to methods for increasing the efficiency of microbial growth and production of products, such as alcohols and acids by microbial fermentation of substrates containing carbon monoxide. More particularly the invention relates to the provision of an alternative sulphur source to a liquid nutrient medium, such that sulphur is available to one or more microorganisms of the fermentation.
The invention relates to a process for optimizing the production of ethanol by microbial fermentation, particularly microbial fermentation of substrates comprising CO. The process involves monitoring the hydrogen production by the microorganism, determining an optimum hydrogen production and adjusting the substrate supply rate in response to a change in the hydrogen production thereby keeping the hydrogen production within a desired range.
Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.
C12P 1/00 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymes; General processes for the preparation of compounds or compositions by using microorganisms or enzymes
C12P 7/18 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
C10G 11/18 - Catalytic cracking, in the absence of hydrogen, of hydrocarbon oils with preheated moving solid catalysts according to the "fluidised bed" technique
C10K 3/02 - Modifying the chemical composition of combustible gases containing carbon monoxide to produce an improved fuel, e.g. one of different calorific value, which may be free from carbon monoxide by catalytic treatment
C12M 1/107 - Apparatus for enzymology or microbiology with means for collecting fermentation gases, e.g. methane
C12M 1/00 - Apparatus for enzymology or microbiology
C01B 3/38 - Production of hydrogen or of gaseous mixtures containing hydrogen by reaction of gaseous or liquid organic compounds with gasifying agents, e.g. water, carbon dioxide, air by reaction of hydrocarbons with gasifying agents using catalysts
C10G 9/36 - Thermal non-catalytic cracking, in the absence of hydrogen, of hydrocarbon oils by direct contact with inert preheated fluids, e.g. with molten metals or salts with heated gases or vapours
44.
Process for production of alcohols by microbial fermentation
The invention provides method for producing butanediol and specifically 2,3-butanediol by anaerobic fermentation of a gaseous substrate comprising CO by a culture of at least one micro-organism. In accordance with particular methods of the invention, 2,3-butanediol is produced by anaerobic fermentation of substrates including carbohydrates and carbon monoxide. The invention further provides for the up-regulation of a native 2,3-butanediol dehydrogenase gene in the at least one micro-organism. The 2,3-butanediol can be further converted to compounds such as butene(s), butadiene and methyl ethyl ketone. These compounds can be further converted to chemical products such as 2-butanol.
The invention provides methods for producing Lactate by anaerobic Fermentation. According to particular methods of the invention, Lactate is produced by anaerobic fermentation of a substrate comprising hydrogen and carbon monoxide.
C12N 15/00 - Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
The present invention relates to methods for sustaining a microbial culture during periods of limited substrate supply. In accordance with the methods of the invention, a microbial culture comprising carboxydotrophic bacteria can be sustained during periods of limited substrate supply by maintaining the temperature of the microbial culture at a temperature below an optimum operating temperature. Examples of periods of limited substrate supply include when the microbial culture is transported to a remote location or during time when it is stored.
2 to a primary bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce an acid such as acetate. The acid, e.g. acetate from the primary bioreactor is then provided to a secondary bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.
C12P 7/64 - Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
Clostridium autoethanogenum is described which has improved efficiency in the production of ethanol by anaerobic fermentation of substrates comprising carbon monoxide. The bacterium can produce ethanol and acetate at an ethanol to acetate ratio of at least 1.0 and has a productivity of at least 1.2 g of ethanol/l of fermentation broth per day. The bacterium is also characterized in that it has substantially no ability to sporulate.
Methods for producing an ester from a gas comprising carbon monoxide. More specifically the invention provides for methods for producing one or more products by the anaerobic fermentation of a gaseous substrate comprising CO and the subsequent conversion of the one or more products to an ester in a down-stream reaction. The invention further provides methods for improving the efficiency of the production of esters from gaseous substrates.
Methods for improving the efficiency of 2,3-butanediol fermentations are disclosed. More specifically methods of increasing the butanediol productivity from the anaerobic fermentation of a substrate comprising carbon monoxide or carbon monoxide and hydrogen by one or more caboxydotrophic acetogenic bacteria are disclosed. The method includes supplying a hydrogen depleted substrate to increase butanediol productivity. The method includes producing butanediol at a volumetric productivity rate of at least 15 g/L/day.
The invention provides, inter alia, methods for the production of acetone, isopropanol and/or precursors of acetone and/or isopropanol by microbial fermentation of substrates comprising CO, genetically modified microorganisms of use in such methods, nucleic acids suitable for preparation of genetically modified microorganisms, a novel alcohol dehydrogenase and nucleic acids encoding same.
C12P 7/04 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
C12N 15/00 - Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
Provided is an inkjet recording apparatus capable of recording a high quality image without limiting the kinds of ink or recording media, including an inkjet head having a plurality of nozzles to eject ink droplets, wherein the ink droplets ejected from the plurality of the nozzles is charged by applying voltage between the inkjet head and the recording medium, and land on the recoding medium so as to record the image on the recording medium, wherein after a first ink droplet lands on the recording medium, a second ink droplet lands on the recording medium so as to overlap with the first ink droplet before discharging of the first ink droplet is completed.
B41J 2/06 - Ink jet characterised by the jet generation process generating single droplets or particles on demand by electric or magnetic field
B41J 2/41 - Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed characterised by selective supply of electric current or selective application of magnetism to a printing or impression-transfer material for electrostatic printing
B41J 2/095 - Ink jet characterised by jet control for many-valued deflection electric field-control type
The present invention relates to improvement in efficiency in gasification for use with syngas fermentation. In particular, the invention relates to increasing the overall carbon capture efficiency of a gasification/fermentation process to produce products such as alcohols.
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymes; General processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
C12M 1/00 - Apparatus for enzymology or microbiology
54.
Optimised media containing nickel for fermentation of carbonmonoxide
The invention relates to improvements in the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of substrates comprising CO. It more particularly relates to the provision of an improved fermentation media, comprising nickel, to a fermentation system such that one or more micro-organisms convert a substrate comprising CO to one or more alcohols, such as ethanol. In particular embodiments, a microbial culture is provided with at least 10 μM nickel, such that CO uptake by the microbial culture increases and ethanol productivity improves.
C12P 7/08 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
A62D 3/02 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
The invention relates to methods for improving the efficiency of carbon capture in microbial fermentation of a gaseous substrate comprising CO and/or H2. In certain aspects the invention relates to improving the efficiency of carbon capture in the microbial fermentation of gaseous substrate comprising CO and/or H2 to produce alcohol(s) and/or acid(s). In particular the invention relates to methods for improving the efficiency of carbon capture in carboxydotrophic fermentation.
The invention relates to the microbial fermentation of gaseous substrates, particularly to gas/liquid contact modules and bioreactors configured to improve the efficiency of fermentations, particularly microbial fermentations of substrates comprising CO. In a particular embodiment, a gas/liquid contact module with multiple channels is configured to produce products in a liquid fermentation broth. In a further particular embodiment, there is provided a method of fermentation of a gaseous substrate to produce a product in a liquid fermentation broth.
The invention relates to the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of substrates comprising CO. It more particularly relates to processes for the production of alcohols from their corresponding acids in the presence of a substrate comprising CO. In particular embodiments, a fermentation reaction producing acid(s) and optionally alcohol(s) is perturbed such that at least a portion one or more of acid(s) is converted to alcohol.
C12P 1/00 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymes; General processes for the preparation of compounds or compositions by using microorganisms or enzymes
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymes; General processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
The invention relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO. The methods of the invention include converting CO to one or more products including alcohols and/or acids and optionally capturing CO2 to improve overall carbon capture. In certain aspects, the invention relates to processes for producing alcohols, particularly ethanol, from industrial waste streams, particularly steel mill off-gas.
A novel class of bacteria is described which has improved efficiency in the production of thanol by anaerobic fermentation of substrates containing carbon monoxide.
The invention relates to biological processes for producing one or more desired products, including alcohols such as ethanol and butanol. The processes comprise carrying out first and second fermentations of substrates in first and second bioreactors, wherein each fermentation produces one or more desired products and/or one or more by-products that can be utilized in the other fermentation. A product and/or by-product of the first fermentation is introduced to the second bioreactor during the fermentation, and a product and/or by-product of the second fermentation is introduced to the first bioreactor during the fermentation.
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymes; General processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
The invention relates to methods for the production of alcohols by microbial fermentation, particularly microbial fermentation of substrates comprising glycerol and/or acetate, to butanol.
C07C 27/04 - Processes involving the simultaneous production of more than one class of oxygen-containing compounds by reduction of oxygen-containing compounds
The present invention relates to methods for increasing the efficiency of anaerobic fermentation processes (1) that produce acetate as a by-product in addition to a desired product, and (2) that can utilize hydrogen and/or carbon dioxide in the fermentation. The method comprises the steps of converting acetate produced by the fermentation process into hydrogen gas and carbon dioxide gas, and utilizing hydrogen gas and/or carbon dioxide gas obtained from the acetate conversion in the anaerobic fermentation process. In particular aspects, the invention relates to processes of producing alcohols, particularly ethanol.
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