Abstract

This document provides genetically modified animals (e.g., mice), humanized heavy chain antibodies, humanized nanobodies, and methods of making and using the same. For example, genetically engineered non-human animals (e.g., genetically engineered mice) that can be designed to produce heavy chain antibodies that can be used to generate a single domain antibody or nanobody are provided.

IPC Classes  ?

• A01K 67/0278 - Knock-in vertebrates, e.g. humanised vertebrates
• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Abstract

This document relates to methods and materials involved in producing antibodies (e.g., single domain antibody (sdAbs) and/or heavy chain only antibodies) having one or two chimeric heavy chains. For example, (A) genetically engineered non-human animals (e.g., genetically engineered mice) having the ability to produce an antibody having one or two chimeric heavy chains that include (1) a non-human Ig heavy chain constant domain (CH) 2 and/or a non-human CH3 domain and (2) a VH domain such as a VH domain set forth in any one of SEQ ID NOs:74-87, (B) genetically engineered non-human animals (e.g., genetically engineered mice) having the ability to produce antibody-like molecules that include (1) a non-human heavy chain constant (CH) 2 domain and/or a non-human CH3 domain (e.g., endogenous CH2 and/or CH3 domains) and (2) a TCR variable domain (e.g., a human TCR variable domain), (C) genetically engineered non-human animals (e.g., genetically engineered mice) having the ability to produce antibody-like molecules that include (a) a first amino acid sequence of a FN3 polypeptide (e.g., a 10FN3 polypeptide), (b) a human Ig variable D domain, (c) a second amino acid sequence of a FN3 polypeptide (e.g., a 10in vivoin vivo affinity maturation are provided.

IPC Classes  ?

• A01K 67/0275 - Genetically modified vertebrates, e.g. transgenic
• C07K 14/705 - ReceptorsCell surface antigensCell surface determinants
• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Abstract

This document provides genetically modified animals (e.g., mice), humanized heavy chain antibodies, humanized nanobodies, and methods of making and using the same. For example, genetically engineered non-human animals (e.g., genetically engineered mice) that can be designed to produce heavy chain antibodies that can be used to generate a single domain antibody or nanobody are provided.

IPC Classes  ?

• A01K 67/0276 - Knock-out vertebrates
• C07K 14/705 - ReceptorsCell surface antigensCell surface determinants
• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• C07K 16/24 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
• C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
• C07K 19/00 - Hybrid peptides
• C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 15/09 - Recombinant DNA-technology
• C12N 15/13 - Immunoglobulins
• C12N 15/62 - DNA sequences coding for fusion proteins

Abstract

This document provides genetically modified animals (e.g., mice), humanized heavy chain antibodies, humanized nanobodies, and methods of making and using the same. For example, genetically engineered non-human animals (e.g., genetically engineered mice) that can be designed to produce heavy chain antibodies that can be used to generate a single domain antibody or nanobody are provided.

IPC Classes  ?

• A01K 67/027 - New or modified breeds of vertebrates
• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• C12N 15/09 - Recombinant DNA-technology
• C12N 15/13 - Immunoglobulins