A method to obtain an alcohol or alcohol precursor from a pretreated lignocellulosic biomass, wherein said process comprising the steps of: - providing a lignocellulosic biomass - contacting said lignocellulosic biomass to a modified Caro's acid composition for a period of time necessary to remove more than 98.5 % of the lignin present in said lignocellulosic biomass and thus obtaining a solid stream and a liquid stream, - exposing said solid stream to a live ethanologenic organism capable of expressing at least one gene for cellulose degradation; and - optionally, recovering said alcohol or alcohol precursor from the cells and/or spent media.
A method of producing an organosulfur compound from a prokaryotic cell wherein said method comprises the steps of: a. providing a live prokaryotic cell capable of expressing at least one gene for the production of said organosulfur compound; b. exposing said live prokaryotic cell to a culture media with a pH of between 4 and 11 containing a carbon source and a sulfur source thereby creating an incubation mixture; c. incubating said live prokaryotic cell in said incubation mixture under aerobic or anaerobic conditions at a temperature ranging from 0 °C to 60 °C for a period of time sufficient for the expression of said at least one gene for the production of said organosulfur compound; d. recovering said organosulfur compound from the bacterial cells and/or spent media; and e. optionally, re-exposing said live prokaryotic cell to an unused media or spent media for the continuous production of said organosulfur compound of interest.
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
C12P 11/00 - Preparation of sulfur-containing organic compounds
C12P 13/00 - Preparation of nitrogen-containing organic compounds
3.
IMPROVED TAURINE BIOSYNTHESIS USING GENETICALLY MODIFIED BACTERIA
A genetically modified prokaryotic cell comprising: at least one of the following: i. an addition, deletion and/or alteration of at least one gene to promote to taurine production; and ii. an addition, deletion and/or alteration of at least one gene related to taurine cellular transportation; and - at least one of the following polynucleotide sequences: i. a vanin (vnn) polynucleotide sequence selected from the group consisting of: vanin-1 (vnn1), wherein said vnn1 polynucleotide sequence has at least 70% sequence coverage to SEQ 3 or SEQ 98, and at least 70% sequence identity to SEQ 3 or SEQ 98; vanin-2 (vnn2), wherein said vnn2 polynucleotide sequence has at least 70% sequence coverage to SEQ 100, and at least 70% sequence identity to SEQ 100; and vanin-3 (vnn3), wherein said vnn3 polynucleotide sequence has at least 70% sequence coverage to SEQ 141, and at least 70% sequence identity to SEQ 141; ii. a cysteamine dioxygenase (ado) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1, and at least 70% sequence identity to SEQ 1; and iii. a flavin-containing monooxygenase 1 (fmo1) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5 or SEQ 99, and at least 70% of sequence identity to SEQ 5 or SEQ 99.
A method of producing a sulfur-containing compound from a prokaryotic cell wherein said method comprises the steps of: - providing a live prokaryotic cell capable of expressing at least one gene for the production of said sulfur-containing compound; - exposing said live prokaryotic cell to a culture media with a pH of between 4 and 11 containing a sugar source and a sulfur source thereby creating an incubation mixture; - incubating said live prokaryotic cell in said incubation mixture under aerobic and/or anaerobic conditions at a temperature ranging from 0 °C to 60 °C for a period of time sufficient for the expression of said at least one gene for the production of said sulfur-containing compound; - recovering said sulfur-containing compound of interest from the bacterial cells and/or spent media; - optionally, re-exposing said live prokaryotic strain to a unused or spent media for the continuous production of said sulfur-containing compound of interest.
A genetically modified prokaryotic cell which comprises of: - a genetic modification that alters at least one gene that regulate the downregulation, degradation, or enhancement of the synthesis of a sulfur-containing compound; and - at least one of the following polynucleotide sequences: i. a vanin (vnn) polynucleotide sequence selected from the group consisting of: - vanin-1 (vnn1), wherein said vnn1 polynucleotide sequence has at least 70% sequence coverage to SEQ 3 or SEQ 98, and at least 70% sequence identity to SEQ 3 or SEQ 98; - vanin-2 (vnn2), wherein said vnn2 polynucleotide sequence has at least 70% sequence coverage to SEQ 100, and at least 70% sequence identity to SEQ 100; and - vanin-3 (vnn3), wherein said vnn3 polynucleotide sequence has at least 70% sequence coverage to SEQ 141, and at least 70% sequence identity to SEQ 141; ii. a cysteamine dioxygenase (ado) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1, and at least 70% sequence identity to SEQ 1; and iii. a flavin-containing monooxygenase 1 (fmo1) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5 or SEQ 99, and at least 70% of sequence identity to SEQ 5 or SEQ 99.
A method of producing an alcohol or alcohol precursor from a cellulosic material and a genetically modified live ethanologenic organism, wherein the method comprises the steps of:
exposing said genetically modified live ethanologenic organism to a culture media with a pH of between 2 and 9 thereby creating an incubation mixture;
exposing said incubation mixture to a source of cellulose;
incubating said genetically modified live ethanologenic organism in said incubation mixture with said source of cellulose under aerobic and/or anaerobic conditions at a temperature ranging from 0° C. to 60° C. for a first period of time sufficient for the degradation of cellulose, thereby creating a degraded cellulose mixture;
optionally, incubating said genetically modified live ethanologenic organism in said degraded cellulose mixture under aerobic and/or anaerobic conditions at a temperature ranging from 0° C. to 60° C. for a second period of time sufficient for the production of said alcohol or alcohol precursor; and
optionally, recovering said alcohol or alcohol precursor from the cells and/or spent culture media.
wherein said genetically modified live ethanologenic organism comprises at least one of the following polynucleotide sequences in its genome:
i. at least one endoglucanase (cen-like) polynucleotide sequence selected from the group consisting of an endoglucanase A-like (cenA-like) polynucleotide sequence, an endoglucanase B-like (cenB-like) polynucleotide sequence and an endoglucanase C-like (cenC-like) polynucleotide sequence;
ii. an exoglucanase (cex-like) polynucleotide sequence; and
iii. a β-glucosidase 1 (bgl1) polynucleotide sequence;
wherein said source of cellulose has a lignin content of at most 1 wt. % and a hemicellulose content of at most 15 wt. %, and wherein said live ethanologenic organism belongs to a genus is selected from the group consisting of: Aspergillus, Mucor, Zymomonas, Escherichia, Clostridia, Bacillus, and Pseudomonas.
A method of producing an alcohol or alcohol precursor from a cellulosic material and a genetically modified ethanologenic organism. The genetically modified organism comprises at least one endoglucanase (cen-like), an exoglucanase (cex-like) or beta-glucosidase 1 (bgl1).
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
C12N 1/22 - Processes using, or culture media containing, cellulose or hydrolysates thereof
C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
C12N 15/56 - Hydrolases (3) acting on glycosyl compounds (3.2), e.g. amylase, galactosidase, lysozyme
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
8.
TAURINE BIOSYNTHESIS USING GENETICALLY MODIFIED BACTERIA
A genetically modified prokaryotic cell comprising:
at least one of the following:
i. an addition, deletion and/or alteration of at least one gene to promote to taurine production; and
ii. an addition, deletion and/or alteration of at least one gene related to taurine cellular transportation; and
at least one of the following polynucleotide sequences:
i. a vanin (vnn) polynucleotide sequence selected from the group consisting of:
vanin-1 (vnn1), wherein said vnn1 polynucleotide sequence has at least 70% sequence coverage to SEQ 3 or SEQ 98, and at least 70% sequence identity to SEQ 3 or SEQ 98;
vanin-2 (vnn2), wherein said vnn2 polynucleotide sequence has at least 70% sequence coverage to SEQ 100, and at least 70% sequence identity to SEQ 100; and
vanin-3 (vnn3), wherein said vnn3 polynucleotide sequence has at least 70% sequence coverage to SEQ 141, and at least 70% sequence identity to SEQ 141;
ii. a cysteamine dioxygenase (ado) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1, and at least 70% sequence identity to SEQ 1; and
iii. a flavin-containing monooxygenase 1 (fmo1) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5 or SEQ 99, and at least 70% of sequence identity to SEQ 5 or SEQ 99.
C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
A method of producing an organosulfur compound from a prokaryotic cell wherein said method comprises the steps of:
a. providing a live prokaryotic cell capable of expressing at least one gene for the production of said organosulfur compound;
b. exposing said live prokaryotic cell to a culture media with a pH of between 4 and 11 containing a carbon source and a sulfur source thereby creating an incubation mixture;
c. incubating said live prokaryotic cell in said incubation mixture under aerobic or anaerobic conditions at a temperature ranging from 0° C. to 60° C. for a period of time sufficient for the expression of said at least one gene for the production of said organosulfur compound;
d. recovering said organosulfur compound from the bacterial cells and/or spent media; and
e. optionally, re-exposing said live prokaryotic cell to an unused media or spent media for the continuous production of said organosulfur compound of interest.
A genetically modified prokaryotic cell which comprises: - a vanin (vnn) polynucleotide sequence selected from the group consisting of: i. vanin-1 (vnn1), wherein said vnn1 polynucleotide sequence has at least 70% sequence coverage to SEQ 3 or SEQ 98, and at least 70% sequence identity to SEQ 3 or SEQ 98; ii. vanin-2 (vnn2), wherein said vnn2 polynucleotide sequence has at least 70% sequence coverage to SEQ 100, and at least 70% sequence identity to SEQ 100; and iii. vanin-3 (vnn3), wherein said vnn3 polynucleotide sequence has at least 70% sequence coverage to SEQ 141, and at least 70% sequence identity to SEQ 141; or - a cysteamine dioxygenase (ado) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1, and at least 70% sequence identity to SEQ 1; and - a flavin-containing monooxygenase 1 (fmo1) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5 or SEQ 99, and at least 70% of sequence identity to SEQ 5 or SEQ 99.
A genetically modified prokaryotic cell to express enzymes involved in the production of taurine. Said prokaryote comprises a vanin selected from: vanin- 1 (vnn-1), vanin-2 (vnn-2), or vanin- 3 (vnn-3), a cysteamine dioxygenase (ado), and a flavin-containing monooxygenase 1 (fmol), in addition to either the addition, alteration, or deletion of at least one gene involved in taurine synthesis or transport.
A process to prepare an aqueous fertilizer with a high nitrogen content, said process comprising the steps of: —providing a solid material having a high nitrogen content; —providing a live yeast in solution adapted to enzymatically removing nitrogen-containing compounds from said solid; —exposing said solid to said yeast in an aqueous environment, thereby creating a metabolically active culture mixture; —incubating said metabolically active culture; —injecting air in to the metabolically active mixture during said incubating step so as to inhibit the production of ethanol; wherein said metabolically active mixture undergoes said incubating step for a period of time sufficient to result in a nitrogen-fed yeast mixture; —hydrolyzing (by autolysis) the resulting nitrogen-fed yeast mixture for a period of time sufficient to remove nitrogen from said yeast, where the nitrogen removed from said yeast is present in the mixture as dissolved nitrogen.
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
C05F 1/00 - Fertilisers made from animal corpses, or parts thereof
C05F 5/00 - Fertilisers from distillery wastes, molasses, vinasses, sugar plant, or similar wastes or residues
A process to make a nitrogen-enhanced yeast-based fertilizer, said process comprising the steps of: —providing a live yeast (BSY) in solution; —exposing said live yeast to a nitrogen-containing compound to a carbohydrate and an enriched nitrogen source such as an amino acid, a protein or the like thereby creating an incubation mixture; —injecting air in to the incubation mixture so as to inhibit the production of ethanol; wherein said incubation mixture undergoes incubation for a period of time sufficient for said yeast to metabolize said nitrogen source and for said yeast to propagate and store the supplied nitrogen source in their vacuoles resulting in a nitrogen-fed yeast mixture; —hydrolyzing (or autolyzing) the resulting nitrogen-fed yeast mixture under specific conditions; and —optionally followed by a dehydration or evaporation step, to meet pre-determined specifications.
C05F 11/08 - Organic fertilisers containing added bacterial cultures, mycelia or the like
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
ABSTRACT A genetically modified prokaryotic cell which comprises: a cysteamine dioxygenase (ADO) polypeptide sequence which has at least 70% sequence coverage to SEQ 2, and at least 25 % sequence identity to SEQ 2; and a vanin (VNN) polypeptide sequence selected from the group consisting of: a vanin- 1 (VNN1) polypeptide sequence which has at least 70% sequence coverage to SEQ 4, and at least 25 % sequence identity to SEQ 4; a vanin-2 (VNN2) polypeptide sequence which has at least 70% sequence coverage to SEQ 84, and at least 25 % sequence identity to SEQ 84; and a vanin-3 (VNN3) polypeptide sequence which has at least 70% sequence coverage to SEQ 128 and at least 25 % sequence identity to SEQ 128.
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
A genetically modified ethanologenic organism which comprises: a. an exoglucanase (cex-like) polynucleotide sequence with at least 70% sequence coverage to SEQ 1 or SEQ 68, and at least 70% sequence identity to SEQ 1 or SEQ 68; and b. a P-glucosidase 1 (bgl!) polynucleotide sequence with at least 70% sequence coverage to SEQ 3 or SEQ 14, and at least 70% sequence identity to SEQ 3 or SEQ 14.
A method for degrading naphthenic acid compounds present in naphthenic acid compounds-containing material into at least one compound of lower toxicity, said method comprising: -providing said naphthenic acid compounds-containing material; -exposing said naphthenic acid compounds-containing material to a modified Caro's acid composition for a period of time sufficient to degrade substantially all of the naphthenic acid compounds present in the naphthenic acid compound-containing material; -optionally, testing the treated material and assess a level of naphthenic acid compounds; and -optionally, releasing the treated material into a waterway.
A62D 3/36 - Detoxification by using acid or alkaline reagents
A62D 3/38 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by reacting with chemical agents by oxidationProcesses for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by reacting with chemical agents by combustion
A62D 101/28 - Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
18.
IMPROVED TAURINE BIOSYNTHESIS USING GENETICALLY MODIFIED BACTERIA
ABSTRACT A genetically modified prokaryotic cell which comprises: a vanin (vnn) polynucleotide sequence selected from the group consisting of: i. vanin-1 (vnnl), wherein said vnnl polynucleotide sequence has at least 70% sequence coverage to SEQ 3 or SEQ 98, and at least 70% sequence identity to SEQ 3 or SEQ 98; ii. vanin-2 (vnn2), wherein said vnn2 polynucleotide sequence has at least 70% sequence coverage to SEQ 100, and at least 70% sequence identity to SEQ 100; and iii. vanin-3 (vnn3), wherein said vnn3 polynucleotide sequence has at least 70% sequence coverage to SEQ 141, and at least 70% sequence identity to SEQ 141; or a cysteamine dioxygenase (ado) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1, and at least 70% sequence identity to SEQ 1; and a flavin-containing monooxygenase 1 (final) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5 or SEQ 99, and at least 70% of sequence identity to SEQ 5 or SEQ 99.
A genetically modified ethanologenic organism which comprises: at least one endoglucanase (cen-like) polynucleotide sequence selected from the group consisting of: an endoglucanase A-like (cenA-like) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1; and at least 70% sequence identity to SEQ 1; an endoglucanase B-like (cenB-Uke) polynucleotide sequence which has least 70% sequence coverage to SEQ 3; and at least 70% sequence identity to SEQ 3; and an endoglucanase C-like (cenC-like) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5; and at least 70% sequence identity to SEQ 5; a P-glucosidase 1 (bgl!) polynucleotide sequence which has at least 70% sequence coverage to SEQ 9 or SEQ 20; and at least 70% sequence identity to SEQ 9 or SEQ 20; and an exoglucanase (cex-like) polynucleotide sequence which has least 70% sequence coverage to SEQ 7 or SEQ 147; and at least 70% sequence identity to SEQ 7 or SEQ 147.
A process to make a yeast-based fertilizer composition from a combination of Brewer’s Spent Yeast and a second organism, said process comprising the steps of: providing said live Brewer’s Spent yeast material; exposing said live Brewer’s Spent yeast material to said second organism, wherein said second organism is capable of degrading proteins and performing aerobic respiration, thereby creating a live mixed culture; incubating said live mixed culture in the presence of at least one nitrogen-containing compound thereby creating an incubation mixture; optionally incorporating into said incubation mixture at least one additional nutrient to promote the propagation of said live mixed culture; optionally, injecting air into said incubation mixture so as to minimize and/or substantially inhibit the production of ethanol; wherein said incubation mixture undergoes incubation for a period of time sufficient for said live mixed culture to metabolize said at least one nitrogen-containing compound and for said live mixed culture to propagate and resulting in a pre-lysis mixture comprising metabolized nitrogen-containing compounds and said live mixed culture; autolyzing said pre-lysis mixture under specific conditions yielding an autolyzed mixture which comprises said yeast-based fertilizer composition.
C05F 17/10 - Addition or removal of substances other than water or air to or from the material during the treatment
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
A process to make a yeast-based fertilizer composition from a combination of Brewer's Spent Yeast and a second organism, said process comprising the steps of: - providing said live Brewer's Spent yeast material; - exposing said live Brewer's Spent yeast material to said second organism, wherein said second organism is capable of degrading proteins and performing aerobic respiration, thereby creating a live mixed culture; - incubating said live mixed culture in the presence of at least one nitrogen-containing compound thereby creating an incubation mixture; - optionally incorporating into said incubation mixture at least one additional nutrient to promote the propagation of said live mixed culture; - optionally, injecting air into said incubation mixture so as to minimize and/or substantially inhibit the production of ethanol; wherein said incubation mixture undergoes incubation for a period of time sufficient for said live mixed culture to metabolize said at least one nitrogen-containing compound and for said live mixed culture to propagate and resulting in a pre-lysis mixture comprising metabolized nitrogen-containing compounds and said live mixed culture; - autolyzing said pre-lysis mixture under specific conditions yielding an autolyzed mixture which comprises said yeast-based fertilizer composition.
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
C05F 5/00 - Fertilisers from distillery wastes, molasses, vinasses, sugar plant, or similar wastes or residues
A process to make a yeast-based fertilizer composition from a combination of Brewer's Spent Yeast and a second organism, said process comprising the steps of:
providing said live Brewer's Spent yeast material;
exposing said live Brewer's Spent yeast material to said second organism, wherein said second organism is capable of degrading proteins and performing aerobic respiration, thereby creating a live mixed culture;
incubating said live mixed culture in the presence of at least one nitrogen-containing compound thereby creating an incubation mixture;
optionally incorporating into said incubation mixture at least one additional nutrient to promote the propagation of said live mixed culture;
A process to make a yeast-based fertilizer composition from a combination of Brewer's Spent Yeast and a second organism, said process comprising the steps of:
providing said live Brewer's Spent yeast material;
exposing said live Brewer's Spent yeast material to said second organism, wherein said second organism is capable of degrading proteins and performing aerobic respiration, thereby creating a live mixed culture;
incubating said live mixed culture in the presence of at least one nitrogen-containing compound thereby creating an incubation mixture;
optionally incorporating into said incubation mixture at least one additional nutrient to promote the propagation of said live mixed culture;
optionally, injecting air into said incubation mixture so as to minimize and/or substantially inhibit the production of ethanol;
A process to make a yeast-based fertilizer composition from a combination of Brewer's Spent Yeast and a second organism, said process comprising the steps of:
providing said live Brewer's Spent yeast material;
exposing said live Brewer's Spent yeast material to said second organism, wherein said second organism is capable of degrading proteins and performing aerobic respiration, thereby creating a live mixed culture;
incubating said live mixed culture in the presence of at least one nitrogen-containing compound thereby creating an incubation mixture;
optionally incorporating into said incubation mixture at least one additional nutrient to promote the propagation of said live mixed culture;
optionally, injecting air into said incubation mixture so as to minimize and/or substantially inhibit the production of ethanol;
wherein said incubation mixture undergoes incubation for a period of time sufficient for said live mixed culture to metabolize said at least one nitrogen-containing compound and for said live mixed culture to propagate and resulting in a pre-lysis mixture comprising metabolized nitrogen-containing compounds and said live mixed culture;
autolyzing said pre-lysis mixture under specific conditions yielding an autolyzed mixture which comprises said yeast-based fertilizer composition.
C05F 11/08 - Organic fertilisers containing added bacterial cultures, mycelia or the like
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
A genetically modified prokaryotic cell which comprises: a cysteamine dioxygenase (ADO) polypeptide sequence which has at least 70% sequence coverage to SEQ 2, and at least 25% sequence identity to SEQ 2; and a vanin (VNN) polypeptide sequence selected from the group consisting of: a vanin-1 (VNN1) polypeptide sequence which has at least 70% sequence coverage to SEQ 4, and at least 25% sequence identity to SEQ 4; a vanin-2 (VNN2) polypeptide sequence which has at least 70% sequence coverage to SEQ 84, and at least 25% sequence identity to SEQ 84; and a vanin-3 (VNN3) polypeptide sequence which has at least 70% sequence coverage to SEQ 128 and at least 25% sequence identity to SEQ 128.
C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
A genetically modified prokaryotic cell which comprises:
a vanin (vnn) polynucleotide sequence selected from the group consisting of:
vanin-1 (vnn1), wherein said vnn1 polynucleotide sequence has at least 70% sequence coverage to SEQ 3 or SEQ 98, and at least 70% sequence identity to SEQ 3 or SEQ 98;
vanin-2 (vnn2), wherein said vnn2 polynucleotide sequence has at least 70% sequence coverage to SEQ 100, and at least 70% sequence identity to SEQ 100; and
vanin-3 (vnn3), wherein said vnn3 polynucleotide sequence has at least 70% sequence coverage to SEQ 141, and at least 70% sequence identity to SEQ 141; or
a cysteamine dioxygenase (ado) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1, and at least 70% sequence identity to SEQ 1; and
a flavin-containing monooxygenase 1 (fmol) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5 or SEQ 99, and at least 70% of sequence identity to SEQ 5 or SEQ 99.
A genetically modified ethanologenic organism which comprises:
a. an exoglucanase (cex-like) polynucleotide sequence with at least 70% sequence coverage to SEQ 1 or SEQ 68, and at least 70% sequence identity to SEQ 1 or SEQ 68; and
b. a β-glucosidase 1 (bg11) polynucleotide sequence with at least 70% sequence coverage to SEQ 3 or SEQ 14, and at least 70% sequence identity to SEQ 3 or SEQ 14.
A genetically modified ethanologenic organism which comprises:
at least one endoglucanase (cen-like) polynucleotide sequence selected from the group consisting of:
an endoglucanase A-like (cenA-like) polynucleotide sequence which has at least 70% sequence coverage to SEQ 1; and at least 70% sequence identity to SEQ 1;
an endoglucanase B-like (cenB-like) polynucleotide sequence which has least 70% sequence coverage to SEQ 3; and at least 70% sequence identity to SEQ 3; and
an endoglucanase C-like (cenC-like) polynucleotide sequence which has at least 70% sequence coverage to SEQ 5; and at least 70% sequence identity to SEQ 5;
a β-glucosidase 1 (bgl1) polynucleotide sequence which has at least 70% sequence coverage to SEQ 9 or SEQ 20; and at least 70% sequence identity to SEQ 9 or SEQ 20; and
an exoglucanase (cex-like) polynucleotide sequence which has least 70% sequence coverage to
cen-likecenA-likecenB-likecenC-likebgl1cex-likecex-like) polynucleotide sequence which has least 70% sequence coverage to SEQ 7 or SEQ 147; and at least 70% sequence identity to SEQ 7 or SEQ 147.
cex-likebgl1bgl1) polynucleotide sequence with at least 70% sequence coverage to SEQ 3 or SEQ 14, and at least 70% sequence identity to SEQ 3 or SEQ 14.
A genetically modified prokaryotic cell which comprises: a cysteamine dioxygenase (ADO) polypeptide sequence which has at least 70% sequence coverage to SEQ 2, and at least 25 % sequence identity to SEQ 2; and a vanin (VNN) polypeptide sequence selected from the group consisting of: a vanin- 1 (VNN1) polypeptide sequence which has at least 70% sequence coverage to SEQ 4, and at least 25 % sequence identity to SEQ 4; a vanin-2 (VNN2) polypeptide sequence which has at least 70% sequence coverage to SEQ 84, and at least 25 % sequence identity to SEQ 84; and a vanin-3 (VNN3) polypeptide sequence which has at least 70% sequence coverage to SEQ 128 and at least 25 % sequence identity to SEQ 128.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Wholesale of, and retail store services related to, chemical
formulas, methods, genetic sequencing, biomass conversion,
life sciences and general industrial chemical development. Research and development relating to the manufacturing of
chemical formulas, methods, genetic sequencing, biomass
conversion, life sciences and general industrial chemical
development; research, development and creating of chemical
formulas, methods, genetic sequencing, biomass conversion,
life sciences and general industrial chemical development.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Wholesale of chemical formulas, methods, genetic sequencing, biomass conversion, life sciences and general industrial chemical development. Research and development relating to the manufacturing of chemical formulas, methods, genetic sequencing, biomass conversion, life sciences and general industrial chemical development; research, development and creating of chemical formulas, methods, genetic sequencing, biomass conversion, life sciences and general industrial chemical development.
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Wholesale of, and retail store services related to, chemical formulas, methods, genetic sequencing, biomass conversion, life sciences and general industrial chemical development
(2) Research, development and manufacturing of chemical formulas, methods, genetic sequencing, biomass conversion, life sciences and general industrial chemical development
A process to prepare anaqueousfertilizer witha high nitrogen content, said process comprising the steps of:-providing a solid material having a high nitrogencontent;- providing a live yeast in solution adapted to enzymatically removing nitrogen-containing QO compounds fromsaid solid; -exposing said solid to said yeast inan aqueous environment, thereby creating a metabolically active culture mixture; - incubating said metabolically active culture; - injecting air in to the metabolically active mixture during said incubating step so as to inhibit the production of ethanol; wherein said metabolically active mixture undergoes said incubating step for a period of time sufficient to result in a nitrogen-fed yeast mixture; - hydrolyzing (by autolysis) the resulting nitrogen-fed yeast mixture for a q period of time sufficient to remove nitrogenfrom saidyeast, where the nitrogen removedfrom said yeast is present inthe mixture as dissolved nitrogen.
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
34.
COMPOSITION FOR USE IN THE DESTRUCTION OF NAPHTHENIC ACIDS
A method for degrading naphthenic acid compounds present in naphthenic acid compounds-containingmaterial into at least one compound of lower toxicity, said method comprising:- providing said naphthenic acid compounds-containing material;- exposing said naphthenic acid compounds-containing material to a modified Caro's acidcomposition for a period of time sufficient to degrade substantially all of the naphthenic acidcompounds present in the naphthenic acid compound-containing material;-optionally, testing the treated material and assess a level of naphthenic acid compounds; and- optionally, releasing the treated material into a waterway.
A62D 3/30 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by reacting with chemical agents
35.
RECYCLING OF ALCOHOL-BASED HAND SANITIZER COMPOSITIONS
There is provided a method of recycling alcohol-based hand sanitizer compositions which comprises the steps of: providing a reaction vessel; adding an amount of hand sanitizer product to said vessel; wherein said hand sanitizer comprises ethanol in an amount ranging from 60 to 95 wt. %; adding a salt in solid form to said hand sanitizer composition; stirring the resulting mixture for a period for a period of time substantially long enough to obtain a precipitate; filtering the precipitate; and recovering a stream of substantially pure ethanol. There is also provided a use of the resulting stream of substantially pure ethanol.
A process to make a nitrogen-enhanced yeast-based fertilizer, said process comprising the steps of:- providing a live yeast in solution;- adding said live yeast to a nitrogen-containing compound and to a carbohydrate thereby creating an incubation mixture;- injecting air in to the incubation mixture so as to inhibit the production of ethanol;wherein said incubation mixture undergoes incubation for a period of time sufficient for said yeast to metabolize said nitrogen-containing compound and for said yeast to propagate and store a resulting supplied nitrogen source in their vacuoles resulting in a nitrogen-fed yeast mixture;- hydrolyzing the resulting nitrogen-fed yeast mixture under specific conditions; and- optionally followed by a dehydration or evaporation step, to meet pre-determined specifications.
C05F 17/00 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
C05F 17/20 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
Composition for use as a hard surface cleaner, sanitizer and/or disinfectant, said composition comprising: an arylsulfonic acid; a source of peroxide; an effective amount of a stabilizer; and water.
A modified aqueous acid composition comprising: sulfuric acid; a compound comprising an amine moiety and a sulfonic acid moiety; and a peroxide; wherein sulfuric acid, said compound comprising an amine moiety and a sulfonic acid moiety and said peroxide are present in a molar ratio of no less than 1: 1: 1. Also disclosed are methods of using such compositions for the decomposition of toxic chemicals such as cyanides.
A modified aqueous acid composition comprising: sulfuric acid; a compound comprising an amine moiety and a sulfonic acid moiety; and a peroxide; wherein sulfuric acid, said compound comprising an amine moiety and a sulfonic acid moiety and said peroxide are present in a molar ratio of no less than 1:1:1. Also disclosed are methods of using such compositions for the decomposition of toxic chemicals such as cyanides.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Industrial chemicals, namely, chemicals synthesized from industrial and waste plant-based biomass exclusively for use in the aircraft industry for fuels.
05 - Pharmaceutical, veterinary and sanitary products
35 - Advertising and business services
Goods & Services
(1) Disinfectants, namely, all purpose disinfectants, disinfectant soaps, disinfectants for sterilization, disinfecting handwash; Hand-sanitizing preparations; Hand sanitizing preparations for hospital use; Surface sanitizing preparations for hospital use; Sanitizing preparations for household use, namely, all purpose sanitizers; Hand sanitizing preparations for institutional and industrial use; Surface sanitizing preparations for institutional and industrial use; Disposable sanitizing wipes; Disposable hand sanitizing wipes (1) Distributorship services in the field of disinfectants, cleaners and sanitizing preparations
01 - Chemical and biological materials for industrial, scientific and agricultural use
37 - Construction and mining; installation and repair services
Goods & Services
Extraction chemicals; Chemicals used in oil drilling; Chemicals for use in industry; Acids. Oil and gas drilling; Extraction of oil; Extraction of gas.
