Abstract

The present invention provides a novel protein that can be used in modifying target DNA. A protein according to the present disclosure is a protein (a1), a protein (a2), or a protein (a3). (a1) is a protein that comprises the amino acid sequence of any one of SEQ ID NO: 1-19. (a2) is a protein that comprises an amino acid sequence obtained by deleting, inserting, substituting, or adding one or more amino acids in the amino acid sequence of any one of SEQ ID NO: 1-19, and that has nuclease activity. (a3) is a protein that comprises an amino acid sequence having not less than 80% identity with the amino acid sequence of any one of SEQ ID NO: 1-19, and that has nuclease activity.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• A01K 67/0275 - Genetically modified vertebrates, e.g. transgenic
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 15/09 - Recombinant DNA-technology
• C12N 15/55 - Hydrolases (3)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression

IPC Classes  ?

• A01K 67/027 - New or modified breeds of vertebrates
• C12N 15/12 - Genes encoding animal proteins

Abstract

Provided is a method capable of sexing birds. A method for sexing birds according to the present disclosure includes a step for sexing birds in a population of bird eggs on the basis of the eye color of each bird in the population of eggs. When the bird in the egg is male, the male bird has a loss-of-function pigment expression gene and a wild-type eye pigment expression gene in a heterozygous form for the eye pigment expression gene on the Z chromosome. When the bird in the egg is female, the female bird has a loss-of-function pigment expression gene for the eye pigment expression gene on the Z chromosome.

IPC Classes  ?

• A01K 67/027 - New or modified breeds of vertebrates
• A01K 35/00 - Marking poultry or other birds
• C12N 15/12 - Genes encoding animal proteins

Abstract

A mutant MAD7 protein with higher target-site cleavage activity is provided. The mutant MAD7 protein includes an amino acid sequence B obtained by introducing mutations into an amino acid sequence A represented by SEQ ID NO:1, the amino acid sequence B including amino-acid substitution mutations of K169 and D529 in the amino acid sequence A.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

The present invention provides: a non-human animal having a novel fiber; and a method for producing the non-human animal. Specifically, the present invention provides: a non-human animal with reduced expression or functions of at least one gene constituting a Krt or KAP family; and a method for producing the non-human animal.

IPC Classes  ?

• A01K 67/027 - New or modified breeds of vertebrates
• D02G 3/02 - Yarns or threads characterised by the material or by the materials from which they are made
• D03D 15/233 - Woven fabrics characterised by the material, structure or properties of the fibres, filaments, yarns, threads or other warp or weft elements used characterised by the material of the fibres or filaments constituting the yarns or threads protein-based, e.g. wool or silk
• D04B 1/14 - Other fabrics or articles characterised primarily by the use of particular thread materials
• D04B 21/00 - Warp knitting processes for the production of fabrics or articles not dependent on the use of particular machinesFabrics or articles defined by such processes
• C12N 15/09 - Recombinant DNA-technology
• C12N 15/12 - Genes encoding animal proteins

Abstract

Provided is a mutant MAD7 protein demonstrating enhanced breakage activity to a target site. This mutant MAD7 protein contains an amino acid sequence B resulting from mutation of an amino acid sequence A with Sequence Number 1, the amino acid sequence B including amino acid substitution mutations at K169 and D529 in the amino acid sequence A.

IPC Classes  ?

• C12N 15/55 - Hydrolases (3)
• A61K 35/12 - Materials from mammalsCompositions comprising non-specified tissues or cellsCompositions comprising non-embryonic stem cellsGenetically modified cells
• A61K 38/46 - Hydrolases (3)
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
• A61P 43/00 - Drugs for specific purposes, not provided for in groups
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 15/09 - Recombinant DNA-technology