In conventionally researched soil-based cultivation and hydroponic cultivation, there have been limitations on increases in dry ear weight and in-ear secondary metabolite content that can be achieved by adjustments to fertilization and other horticultural management technology, soil selection, growing environment temperature, horticultural lighting intensity and quality, photoperiod, and drought treatments during the reproductive growth period of the plant. Used in the present invention is a system in which at least a portion of plant roots adhere to a hydrophilic film surface, and a nourishing solution is disposed to as to contact the reverse surface of the hydrophilic film which the plant roots adhere. Switching between the vegetative growth period and reproductive growth period of the plant is performed via a change in day length. A cultivation system and cultivation method according to the present invention enable cultivation wherein the growth of ears in which a secondary metabolite accumulates is promoted, and therefore can be used in the fields of agriculture and pharmaceutical production.
A01G 27/02 - Self-acting watering devices, e.g. for flower-pots having a water reservoir, the main part thereof being located wholly around or directly beside the growth substrate
A01G 31/00 - Soilless cultivation, e.g. hydroponics
2.
PLANT CULTIVATION SYSTEM AND PLANT CULTIVATION METHOD
With all the conventional measures, namely, con- ventional horticultural management techniques (such as fertilizers in soil cultivation and hydroponic cultiva- tion), selection of soil, temperature in the growth en- vironment, intensity and quality of horticultural lighting, photoperiodic cycle, and drought treatment during the reproductive growth period of a plant, the increase in the inflorescence dry weight of the plant and in the content of secondary metabolites in the in- florescence has been limited. The present invention uses a plant cultivation system wherein at least a part of the roots of a plant adheres to one surface of a hydrophilic film, a nutri- ent fluid is disposed to be in contact with the other surface of the hydrophilic film opposite to the film surface to which the plant roots adhere, and a switch- ing between the vegetative growth period and reproduc- tive growth period of the plant is performed by chang- ing the light period. The cultivation system and cultivation method of the present invention makes it possible to promote growth of inflorescences in which secondary metabolites are accumulated, and therefore can be used in the fields of agriculture and the manufacture of pharmaceu- ticals.
The present invention addresses the problem of providing a method for increasing the tissue regeneration capacity of a cell population, a cell population production method including said method, and a cell population produced using said production method. The present invention pertains to (1) a method for increasing the tissue regeneration capacity of a cell population, said method including a step for incubating a cell population in a gel-like thermoreversible polymer solution, (2) a cell population production method including said method, and (3) a cell population produced using said production method.
Prior art technology has a problem in that when a plant is cultivated for a long period of time on a PVA film having its lower surface positioned in contact with a nutrient fluid, plant roots that have formed penetrate through the film. A plant cultivation system wherein the PVA film has an equilibrium degree of swelling in the range of from 125 to 250% as measured in water at 30° C. and has a loss tangent (tan δ) in the range of from 0.005 to 0.2 as measured in an equilibrium swollen state in water at 30° C., and a method for cultivating a plant by using this plant cultivation system. Plant cultivation can be performed for a long period of time while avoiding infection by bacteria and the like causative of plant diseases. Therefore, the present invention is useful in, e.g., agriculture and the manufacture of pharmaceuticals.
A01G 31/00 - Soilless cultivation, e.g. hydroponics
A01G 25/02 - Watering arrangements located above the soil which make use of perforated pipe-lines or pipe-lines with dispensing fittings, e.g. for drip irrigation
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Unprocessed water absorbing polyacrylate resins; artificial plant-cultivating soil containing water absorbing polyacrylate resin; culture growing media for cells or tissues for scientific and research use; chemicals, namely, industrial chemicals, chemicals for treating hazardous waste; plant growth regulating preparations; adhesives for industrial purposes; higher fatty acids for agricultural or industrial purposes; non-metallic minerals, namely, sulphur, huntite; plastic materials, namely, unprocessed plastic materials in powder, liquid or paste form; thermosetting plastic refractory materials; paper and sulphite pulp; flour and starch for industrial purposes; fertilizers; photographic supplies, namely, photographic chemicals, emulsions, chemical test papers; artificial sweeteners; ceramic glazings in the nature of a dry chemical preparation for use in the manufacture of ceramics
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
17 - Rubber and plastic; packing and insulating materials
21 - HouseHold or kitchen utensils, containers and materials; glassware; porcelain; earthenware
29 - Meat, dairy products, prepared or preserved foods
31 - Agricultural products; live animals
Goods & Services
Polymer water absorbent for use in soil for plant cultivation, artificial soil containing water absorbent for plant cultivation, culture media for cells or tissues for scientific and research use, chemicals for use in industry and science, plant growth regulating preparations, adhesives for industrial purposes; higher fatty acids for the manufacture of soap and laundry detergent; non-metallic minerals, namely, sulphur, huntite, diatomite, dolomite, potassium nitrate and bentonite; plastic materials, namely, unprocessed plastic materials in powder, liquid, or paste form; paper pulp; flour and starch for industrial purposes, namely, for use in manufacturing paper and textiles; photographic supplies, namely, photographic chemicals and emulsions; chemical test papers, artificial sweeteners; ceramic glazings in the nature of a dry chemical preparation for use in the manufacture of ceramics Agricultural implements and machines, namely, seeders, hay balers, cultivators, harvesters, tractor-towed plows and reapers Plastic films, tubes, or sheeting for agricultural purposes; semi-worked plastic in the form of pellets, rods, foils, foams, fibers, films and sheets, for use as materials Flower pots; hydroponic apparatus for domestic horticultural purposes, namely, hydroponic garden kit for home use comprising growing containers and also hydroponic fertilizers, seeds, substrate, and drain components Frozen vegetables, frozen fruits, processed vegetables, processed fruits Unprocessed edible seaweed, fresh vegetables, fresh fruits, live trees, live grasses, turf, seedlings, live young plants, flowers, potted dwarfed trees in the nature of bonsai
17 - Rubber and plastic; packing and insulating materials
21 - HouseHold or kitchen utensils, containers and materials; glassware; porcelain; earthenware
29 - Meat, dairy products, prepared or preserved foods
31 - Agricultural products; live animals
Goods & Services
Agricultural implements or machines other than hand-operated, namely, seeders, hay balers, cultivators, harvesters, tractor-towed plows and reapers Plastic films or sheeting for agricultural purposes Hydroponic apparatus for domestic horticultural purposes, namely, hydroponic garden kit for home use comprising growing containers and also hydroponic fertilizers, seeds, substrate, and drain components; flower pots Frozen vegetables, frozen fruits, processed vegetables not including vegetable juices for cooking, processed fruits Seaweed, unprocessed, for human or animal consumption; fresh vegetables; unprocessed fruits; live trees; grass seed; turf, seedlings; young live plants; flowers, potted dwarfed trees in the nature of bonsai
8.
Plant cultivation system and a method for plant cultivation
Prior art technology has a problem in that when a plant is cultivated for a long period of time on a PVA film having its lower surface positioned in contact with a nutrient fluid, plant roots that have formed penetrate through the film. A plant cultivation system wherein the PVA film has an equilibrium degree of swelling in the range of from 125 to 250% as measured in water at 30° C. and has a loss tangent (tan δ) in the range of from 0.005 to 0.2 as measured in an equilibrium swollen state in water at 30° C., and a method for cultivating a plant by using this plant cultivation system. Plant cultivation can be performed for a long period of time while avoiding infection by bacteria and the like causative of plant diseases. Therefore, the present invention is useful in, e.g., agriculture and the manufacture of pharmaceuticals.
A01G 31/00 - Soilless cultivation, e.g. hydroponics
A01G 25/02 - Watering arrangements located above the soil which make use of perforated pipe-lines or pipe-lines with dispensing fittings, e.g. for drip irrigation
9.
PLANT CULTIVATION SYSTEM AND A METHOD FOR PLANT CULTIVATION
[Problems] Prior art technology has a problem in that when a plant is cultivated for a long period of time on a polyvinyl alcohol (PVA) film having its lower surface positioned in contact with a nutrient fluid, plant roots that have formed penetrate through the film. [Means to solve the problems] A plant cultivation system wherein the PVA film has an equilibrium degree of swelling in the range of from 125 to 250 % as measured in water at 30 °C and has a loss tangent (tan 6) in the range of from 0.005 to 0.2 as measured in an equilibrium swollen state in water at 30 °C, and a method for cultivating a plant by using this plant cultivation system. [Industrial applicability] Plant cultivation can be performed for a long period of time while avoiding infection by bacteria and the like causative of plant diseases. Therefore, the present invention is useful in, e.g., agriculture and the manufacture of pharmaceuticals.
[Problem] To solve a problem that, when the bottom surface of a polyvinyl alcohol (PVA)-based film is brought into contact with a nourishing solution and a plant is cultivated on the film over a long period of time, the plant's roots penetrate through the film. [Solution] A system for plant cultivation and a plant cultivation method, characterized in that the PVA-based film has an equilibrium swelling degree in water (at 30oC) of 125-250% inclusive and a loss tangent (tan δ) in an equilibrium swelling state in water (at 30oC) of 0.005-0.2 inclusive. [Industrial applicability] The system and method according to the present invention, which enable plant cultivation over a long period of time while protecting the plant from infection with microorganisms etc. inducing plant diseases, can be used in the fields of agriculture and pharmaceutical industry.
[Problem] To provide: a polyvinyl alcohol film for plant cultivation that can inhibit the penetration of roots and that has excellent nutrient permeability; a method for producing the film; and a method for cultivating plants by using the film. [Solution] The disclosed polyvinyl alcohol film for plant cultivation has an average birefringence value of 4.0×10-3-12.0×10-3 and a swelling degree of 150-180%, the average birefringence value being found by averaging, in the thickness direction, the birefringence rates measured in the machine direction. The disclosed method for producing a polyvinyl alcohol film for plant cultivation involves: a step of stretching a polyvinyl alcohol film having a moisture content of 5-20% by mass to 1.3-1.7 times the original dimension thereof; and a step of thermally treating the stretched film at a temperature within the range of 130-170°C. The disclosed method for cultivating plants involves cultivating a plant in a manner such that the plant is placed in direct contact with said polyvinyl alcohol film for plant cultivation.
A01G 31/00 - Soilless cultivation, e.g. hydroponics
B29C 41/24 - Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of indefinite length
B29C 55/06 - Shaping by stretching, e.g. drawing through a die; Apparatus therefor of plates or sheets uniaxial, e.g. oblique parallel with the direction of feed
[Problem] To provide: a film that has high film strength and is thus capable of inhibit the penetration of roots, that has excellent nutrient permeability and thus promotes the growth of plant bodies, and that is suitable as a film for plant cultivation; a method for producing the film; and a method for cultivating plants by using the film. [Solution] The disclosed film is made by using polyvinyl alcohol and a polycarboxylic acid, in which the carboxyl group(s) thereof may be neutralized, at a mass ratio of from 65:35 to 95:5, and has a softening point of 75 to 90°C. The disclosed method for producing a film involves a step of applying a thermal treatment for 6 to 14 minutes at a temperature within the range of 130 to 170°C to a film prepared from a liquid material that contains polyvinyl alcohol and a polycarboxylic acid, in which the carboxyl group(s) thereof may be neutralized, at a mass ratio of from 65:35 to 95:5. The disclosed method for cultivating plants involves cultivating a plant in a manner such that the plant is placed in direct contact with said film.
To provide a system for producing safe and high nutrition vegetables at low cost.
A plant cultivation system for cultivating a plant on a nonporous hydrophilic film, which comprises a nonporous hydrophilic film and a feeding means for supplying water or a nutrient fluid to the lower surface of the film in the absence of a hydroponic tank for accommodating water or a nutrient fluid and cultivating a plant therein. As the feeding means, use is made of a water absorbing material which is in contact with the film and which is disposed between the film and a water impermeable material. A system for producing safe and high nutrition vegetables at low cost can be obtained by disposing the water impermeable material directly on ground soil, whereupon the water absorbing material and an irrigation tube are disposed, followed by disposing the film thereon.
A01G 13/02 - Protective coverings for plants; Devices for laying-out coverings
A01G 27/02 - Self-acting watering devices, e.g. for flower-pots having a water reservoir, the main part thereof being located wholly around or directly beside the growth substrate
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Water absorbing polyacrylate resin, artificial soil
containing water absorbing polyacrylate resin, culture media
for cells or tissues (except for medical use), chemicals,
plant growth regulating preparations, adhesives (except for
office or home use), higher fatty acids, non-ferrous metals,
non-metallic minerals, plastic materials, pulp, flour and
starch for industrial purposes, fertilizers, photographic
supplies, chemical test papers, artificial sweeteners,
ceramic glazings.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
17 - Rubber and plastic; packing and insulating materials
21 - HouseHold or kitchen utensils, containers and materials; glassware; porcelain; earthenware
29 - Meat, dairy products, prepared or preserved foods
31 - Agricultural products; live animals
Goods & Services
Water absorbent in soil for plant cultivation, artificial
soil containing water absorbent for plant cultivation,
culture media for cells or tissues (except for medical use),
chemicals, plant growth regulating preparations, adhesives
(except for office or home use), higher fatty acids,
non-ferrous metals, non-metallic minerals, plastic
materials, pulp, flour and starch for industrial purposes,
fertilizers, photographic supplies, chemical test papers,
artificial sweeteners, ceramic glazings. Agricultural implements or machines other than
hand-operated. Plastic films or sheeting for agricultural purposes, plastic
semi-worked products (for use as materials). Hydroponic apparatus for domestic horticultural purposes,
flower pots. Frozen vegetables, frozen fruits, processed vegetables,
processed fruits. Edible seaweeds, vegetables, fruits, trees, grasses, turf,
seedlings, young plants, flowers, potted dwarfed trees
(bonsai).
NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY (Japan)
Inventor
Nishihara, Eiji
Mori, Yuichi
Okamoto, Akihiro
Yoshioka, Hiroshi
Abstract
Disclosed is a system for cultivating a plant, in which a contaminated water contaminated by an inorganic salt (e.g., NaCl; which inhibits the growth of the plant) can be purified efficiently by a simple manipulation and at low cost to supply the purified water to the plant. The system for cultivating a plant comprises: a selective moisture-permeable film which is impermeable to water and is permeable to water vapor; a cultivation medium mixture which is arranged on the film and comprises a support material for plant cultivation uses and a water-absorbable resin; and a mulching sheet which is impermeable to water vapor and is arranged on the cultivation medium mixture.
[PROBLEMS] To provide a system whereby a safe and highly nutritious vegetable can be produced at a low cost. [MEANS FOR SOLVING PROBLEMS] A plant cultivation system wherein a plant is grown on a porous hydrophilic film with the use of a means of supplying water or a nutrient solution to the film from the bottom side of the film without providing a water tank containing water or a nutrient solution. As the means of supplying water or a nutrient solution from the bottom side of the film, a water-absorbent material being in contact with the porous hydrophilic film is provided between the porous hydrophilic film and a water-impermeable material. By providing the water-impermeable material, the water-absorbent material, an irrigation tube and the porous hydrophilic film directly on the soil in this order, it becomes possible to establish a system whereby a safe and highly nutritious vegetable can be produced at a low cost.
[PROBLEMS] To provide an instrument for cultivating a plant with which a specific component (for example, nitrate nitrogen) contained in the cultivated plant can be reduced, and a method of cultivating a plant. [MEANS FOR SOLVING PROBLEMS] An instrument having such a shape that a plant to be cultivated can be contained therein. This instrument has at least a reservoir for containing water or a nourishing liquid therein, a film to be provided on the water or the nourishing liquid in the reservoir, and a means of supplying water or the nourishing liquid from above the film. This film is a non-porous hydrophilic film capable of substantially unify with plant roots.
The present inventors have found that corneal limbal stem cells, which can be used to treat diseases associated with cornea can be obtained by a method for cultivating corneal limbal stem cells characterized in that the method comprises the steps of: embedding the corneal limbal tissue within an aqueous solution in a low temperature sol state wherein the aqueous solution contains at least a hydrogel-forming polymer showing thereto-reversible sol-gel transition and wherein the aqueous solution is in a sol state at a low temperature and is in a gel state at a high temperature; heating the said aqueous solution to cultivate the said corneal limbal tissue within the said hydrogel in a high temperature gel state so that the corneal limbal stem cells spread and grow outside of the said corneal limbal tissue; cooling the said hydrogel to return from the gel state to a low temperature sol state; and collecting the spread and grown cells derived from corneal limbal tissue.
Japan as represented by Director-General of National Institute of Infectious Diseases (Japan)
Mebiol Inc. (Japan)
Inventor
Miyamura, Tatsuo
Murakami, Kyoko
Suzuki, Tetsuro
Yoshioka, Hiroshi
Mori, Yuichi
Ohtsubo, Shin-Ya
Abstract
It is intended to provide proliferated HCV with which the replication of HCV gene and the formation and release of HCV particles can be efficiently conducted and a method of proliferating the same. A method of proliferating HCV particles by using a hydrogel-forming polymer the aqueous solution of which is in the state of a fluid sol at a temperature lower than the sol-gel transmission temperature but reversibly converted into a hydrogel at a temperature higher than the sol-gel transmission temperature. At a temperature higher than the sol-gel transmission temperature, a mixture of cells having HCV enclosed therein with an aqueous solution of the hydrogel-forming polymer as described above is gelled and then the cells are cultured in this state.
