A method of manufacturing the antibacterial modified chitosan-based hemostatic material comprising: (i) preparing materials including: a modified chitosan ingredient and third different solutions including a solution containing polysaccharide, a solution containing dissolving solvent, and a solution containing nano silver; (ii) mixing the solutions prepared in step (i) to create a foundation mixture; (iii) loading the foundation mixture into a electrospinning device according to predetermined specifications to obtain the antibacterial modified chitosan-based hemostatic material in the form of nanofibers; and (iv) applying the antibacterial modified chitosan-based hemostatic material to a bleeding tissue on the surface of skin of a subject in need thereof to prevent bleeding and fluid exudation from the tissue, and promoting bacteriostatic and anti-inflammatory effects on the wound surface.
A method of manufacturing a chitosan composition comprising steps performed in the following specific order: (i) preparing materials; (ii) creating a chitin ingredient from shrimp shells; (iii) creating a chitin ingredient from oyster mushroom; (iv) creating a chitin mixture by homogenously mixing the chitin ingredient from shrimp shells with the chitin ingredient from oyster mushroom; (v) loading the chitin mixture into the centrifugal spray drying device having predetermined specifications to create the chitosan composition; and (vi) packaging and preservation.
A method for producing a complex composition of turkey tail mushroom extract—chitosan comprising: (i) preparing materials; (ii) mixing the turkey tail mushroom extract ingredient with the emulsifier ingredient to obtain a first homogeneous mixture; (iii) mixing the first homogeneous mixture with the chitosan solution ingredient to obtain a second homogeneous mixture; and (iv) sonicating the second homogeneous mixture to obtain the complex composition of turkey tail mushroom extract—chitosan.