Disclosed herein are methods for making, culturing, and maintaining engineered podocyte-like cells. The methods comprise growing glomerular cells in in vitro conditions to differentiate the glomerular cells into podocyte-like cells. Also disclosed herein are isolated and purified podocyte-like cells and kits comprising the engineered podocyte-like cells and/or the media used to differentiate the podocyte-like cells. The engineered podocyte-like cells can be used in recellularization of a decellularized extracellular matrix. In some instances, disclosed herein are recellularized isolated organs that can comprise the engineered podocyte-like cells.
Mayo Foundation for Medical Education and Research (USA)
Inventor
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abstract
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
Provided herein are methods and compositions relating to at least partially recellularized human organs. Various methods of decellularizing non-human animal organs and recellularizing a non-human animal extracellular matrix with cell compositions. Further provided are compositions and methods for treating a liver or other disease (such as acute liver failure) using an extracorporeal bioengineered liver or other organ.
A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit: a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.
Disclosed herein are methods to maintain or inhibit an alteration in volume or shape of hydrated decellularized extracellular matrix from a mammalian organ or tissue or a portion thereof.
in vitroin vitro conditions to differentiate the glomerular cells into podocyte-like cells. Also disclosed herein are isolated and purified podocyte-like cells and kits comprising the engineered podocyte-like cells and/or the media used to differentiate the podocyte-like cells. The engineered podocyte-like cells can be used in recellularization of a decellularized extracellular matrix. In some instances, disclosed herein are recellularized isolated organs that can comprise the engineered podocyte-like cells.
G01N 33/554 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
The invention provides a method to prepare a graft comprising a recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof, and a method of using the recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof.
05 - Pharmaceutical, veterinary and sanitary products
42 - Scientific, technological and industrial services, research and design
Goods & Services
Biological implants, namely, a vital processed human or animal connective tissue, namely, products derived from organ tissue, namely, porcine, bovine and human organ tissue, for use in surgical and non-surgical repair, enhancement and replacement of human tissue, namely, abdominal wall and hernia repair, breast reconstruction, cardiac repair, wound repair, liver, kidney, pancreas, heart, lung and bone repair Medical and scientific research; product development services in the fields of biotechnology, pharmaceuticals, and medical devices
A system (200) for supporting a patient organ can include a primary circuit (212b), a secondary circuit (212a), and a controller (214). The primary circuit can include an inlet configured to connect to the patient (54), an outlet configured to connect to the patient, and a primary pump (206a). The secondary circuit can be connected to the primary blood circuit and can include an enclosure (202) configured to support an organ (50) therein in a blood flow, a secondary pump, a gas transfer unit (208), and a secondary sensor (238a). The controller can be configured to operate the gas transfer unit based on the secondary sensor signal. The organ may be a bio-engineered organ. In an example, the organ is a liver and operates to perform functions of a liver in place of or in support of the liver of the patient.
A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit; a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.
A system (200) for supporting a patient organ can include a primary circuit (212b), a secondary circuit (212a), and a controller (214). The primary circuit can include an inlet configured to connect to the patient (54), an outlet configured to connect to the patient, and a primary pump (206a). The secondary circuit can be connected to the primary blood circuit and can include an enclosure (202) configured to support an organ (50) therein in a blood flow, a secondary pump, a gas transfer unit (208), and a secondary sensor (238a). The controller can be configured to operate the gas transfer unit based on the secondary sensor signal. The organ may be a bio-engineered organ. In an example, the organ is a liver and operates to perform functions of a liver in place of or in support of the liver of the patient.
A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit; a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
Mayo Foundation for Medical Education & Research, Mayo Medical Ventures (USA)
Inventor
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abstract
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
The disclosure provides for methods of sealing of decellularized and recellularized engineered organ grafts, thereby providing a reinforced (fortified) decellularized and recellularized engineered organ graft.
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
The invention provides for methods and materials to decellularize an organ or portion thereof and to recellularize such a decellularized organ or portion thereof to thereby generate an organ or portion thereof.
Disclosed herein are recellularized livers prepared from decellularized liver extracellular matrices. Also disclosed herein are kits and systems comprising a recellularized liver as described herein. Also disclosed herein are methods of recellularizing livers from decellularized liver extracellular matrices.
A system for testing and transporting a biologically engineered organ can include a housing, a transportable enclosure, a perfusate circuit, and a pump. The housing can be configured to receive and support a biologically engineered organ therein in a perfusate flow. The housing can include a perfusate inlet and a perfusate outlet to receive the perfusate flow through the housing. The transportable enclosure can surround at least a portion of the housing. The perfusate circuit can be connected to the perfusate inlet and the perfusate outlet and can be configured to transmit perfusate through the system. The pump can be connected to the circuit and can be configured to circulate perfusate through the perfusate circuit.
Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
The disclosure provides for methods of sealing of decellularized and recellularized engineered organ grafts, thereby providing a reinforced (fortified) decellularized and recellularized engineered organ graft.
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.
The invention provides a method to prepare a graft comprising a recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof, and a method of using the recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof.
Disclosed herein are recellularized livers prepared from decellularized liver extracellular matrices. Also disclosed herein are kits and systems comprising a recellularized liver as described herein. Also disclosed herein are methods of recellularizing livers from decellularized liver extracellular matrices.
MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH (USA)
Inventor
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abstract
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
Mayo Foundation for Medical Education and Research (USA)
Miromatrix Medical Inc. (USA)
Inventor
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abstract
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
A method to provide an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, and uses thereof, are provided.
A method to provide an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, and uses thereof, are provided.
The invention provides a method for preparing a perfusion based islet cell containing extracellular matrix of liver, an islet cell recellularized extracellular matrix, and methods of using the recellularized matrix.
The invention provides a method for maintaining capillary lumen diameter, reducing a decrease in capillary vessel lumen diameter or expanding capillary vessel lumen diameter in a re-endothelialized decellularized organ or tissue graft with an intact extracellular matrix vascular network. The method is based on administration of endothelial cells and microparticles to the decellularized ECM.
The invention provides a method for maintaining capillary lumen diameter, reducing a decrease in capillary vessel lumen diameter or expanding capillary vessel lumen diameter in a re-endothelialised but otherwise decellularised organ or tissue graft with an intact extracellular matrix (ECM) vascular network. The method is based on administration of endothelial cells and microparticles to the decellularised ECM.
The invention provides a method for preparing a perfusion based islet cell containing extracellular matrix of liver, an islet cell recellularized extracellular matrix, and methods of using the recellularized matrix.
The invention provides a method for preparing a perfusion based islet cell containing extracellular matrix of liver, an islet cell recellularized extracellular matrix, and methods of using the recellularized matrix.
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
The invention provides a method for preparing a perfusion based 3D cell culture system, a recellularized matrix culture system, and methods of using the culture system.
The invention provides for methods and materials to decellularize an organ or portion thereof and to recellularize such a decellularized organ or portion thereof to thereby generate an organ or portion thereof.
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
