Abstract

A manipulation system for microscope applications comprises a micromanipulation tool and a protective device protecting the tool, wherein the tool and the protective device are movable relative to one another between a position protecting the tool in a contactless manner and a position releasing the tool for the manipulation.

IPC Classes  ?

• G02B 21/32 - Micromanipulators structurally combined with microscopes
• G01N 1/28 - Preparing specimens for investigation

Abstract

A(P) taking into account a predetermined tolerance profile ΔZ by the following equation: A(P).

IPC Classes  ?

• G05D 3/00 - Control of position or direction
• G01N 1/28 - Preparing specimens for investigation
• G02B 21/32 - Micromanipulators structurally combined with microscopes

Abstract

An automated high-precision plunger pump for volumes of less than 1 microliter, which is used in the microscope-assisted micromanipulation of biological material, comprises a housing (1) and a pump head (8) that defines a working chamber (9) with a common or separate inlet and outlet opening (10), wherein the volume of the working chamber (9) can be altered by an axially movable plunger (7) that can be introduced into it. The plunger (7) is axially movable via a threaded spindle (5) or a spindle nut (6) arranged in threaded engagement on the threaded spindle (5), and the threaded spindle (5) and/or the spindle nut (6) can be rotationally driven and the respective other component is designed to be rotationally lockable.

IPC Classes  ?

• F04B 19/00 - Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups
• F04B 9/02 - Piston machines or pumps characterised by the driving or driven means to or from their working members the means being mechanical
• A61M 5/315 - PistonsPiston-rodsGuiding, blocking or restricting the movement of the rodAppliances on the rod for facilitating dosing
• H02K 7/06 - Means for converting reciprocating motion into rotary motion or vice versa

Abstract

A manipulation system for microscope applications comprises a micromanipulation tool (1) and a protective device (2) protecting the tool, the tool (1) and the protective device (2) being movable relative each other between a position protecting the tool in a contactless manner and a position releasing the tool for manipulation.

IPC Classes  ?

• G02B 21/32 - Micromanipulators structurally combined with microscopes
• A61M 5/32 - NeedlesDetails of needles pertaining to their connection with syringe or hubAccessories for bringing the needle into, or holding the needle on, the bodyDevices for protection of needles
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• G01N 1/04 - Devices for withdrawing samples in the solid state, e.g. by cutting

Abstract

The invention relates to a method for the collision-free positioning of a micromanipulator tool relative to a sample carrier with the aid of a microscope, comprising the following steps: a) determining the focal position ZP of at least one point P on the surface of the sample carrier by focusing a microscope objective having a first numerical aperture NA1 on the at least one point; b) positioning the micromanipulator tool on the optical axis of the microscope; c) determining the focal position ZM of the micromanipulator tool by focusing the microscope objective or a second microscope objective having a second numerical aperture NA2 smaller than the first numerical aperture NA1 on the tip of the micromanipulator tool; d) calculating the lowering travel ZA(P) while taking a predetermined tolerance profile ΔZ into account, by means of the following equation: ZA(P) = ZM - ZP - ΔZ, where ΔZ = ΔZM + ΔZP, wherein ΔZM and ΔZP is a predetermined tolerance in determining the position of the manipulator tool and of the sample carrier, respectively; and e) positioning the micro manipulator tool at the point P by lowering by the lowering travel ZA(P).

IPC Classes  ?

• G02B 21/32 - Micromanipulators structurally combined with microscopes
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• G01N 1/04 - Devices for withdrawing samples in the solid state, e.g. by cutting

Abstract

An automated high-precision plunger pump for volumes below one microliter which is used for the microscope-assisted manipulation of biological material comprises a housing (1) and a pump head (8) which defines a working chamber (9) having a common or separate inlet and outlet (10), the volume of the working chamber (9) being modifiable by means of an axially movable plunger (7) that can be introduced into the chamber. The plunger (7) is axially movable by means of a threaded spindle (5) or a spindle nut (6) which is in threaded engagement on the threaded spindle (5) and the threaded spindle (5) and/or the spindle nut (6) is designed such that it can be rotationally driven and the other can be rotationally fixed.

IPC Classes  ?

• F04B 19/00 - Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups

Abstract

A dish for cell cultivation and microscopy is provided, comprising a ring and an outer ridge upwardly and axially protruding therefrom, wherein the ring is covered at its bottom side with a transparent membrane and, thus, forms a recess delimited by the inner side of the ring and the membrane, characterized in that the recess is filled with an adhesive filling. Alternatively, a dish for cell cultivation and microscopy is provided, comprising a base and an outer ridge protruding upwardly and axially at its rim, wherein the base and the outer ridge are made of a transparent plastic, the base together with the inner side of the outer ridge forms a recess filled with an adhesive filling, and the thickness of the base and the filling together is less than about 1 mm.

IPC Classes  ?

• C12M 1/22 - Petri dishes