A method to detect the presence of parent brevetoxin (BT) and brevetoxin metabolites (BTXs) in shellfish and aquatic samples is provided. The method may include contacting a sample with a binding molecule comprising an aptamer, and determining if the binding molecule binds a Brevetoxin antigen in the sample.
A method for harmful algal bloom (HAB) mitigation is provided. The method may include preparing a HAB mitigation substance comprising a curcuminiod substance, and applying the HAB mitigation substance to a HAB to destroy algal cells within the HAB and prevent or inhibit release of toxins from the algal cells.
A01N 35/02 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio-analogues thereofDerivatives thereof, e.g. acetals
Affinity reagents are described, including antibodies and aptamers that are cross- reactive with one or more A. ocellatum or P. pillulare antigens. The aptamers and antibodies can detect these antigens in marine organisms or aquaculture aquarium settings, indicative of infection such as velvet disease infestation. The results may be used to monitor the health and disease of captured or cultured fish species or the safety of water to be introduced into an aquaculture facility.
C12N 15/115 - Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/6811 - Selection methods for production or design of target specific oligonucleotides or binding molecules
Harmful algal blooms (HABs) such as red tide blooms can be mitigated or controlled by applying a water-soluble algicidal unsaturated fatty acid salt or soap, or a methanol-free solution of an unsaturated algicidal fatty acid, to water containing a HAB concentration, wherein the salt, soap or solution reduces the HAB concentration.
Velvet disease infestation is detected using affinity reagents that are cross-reactive with one or more A. ocellatum or P. pillulare antigens. The analysis may be performed shipboard, dockside, in an aquaculture or aquarium setting, otherwise in situ at the point of sample collection or elsewhere. The results may be used to monitor health and disease of captured or cultured fish species or the safety of water to be introduced into an aquaculture facility.
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
7.
BIOMARKERS FOR PREDICTING IMMINENT OVULATION OR REPRODUCTIVE COMPETENCE IN MARES
Broodmare imminence of ovulation and reproductive competence may be determined by collecting blood, serum or plasma samples from a mare over two or more days in such mare's estrous cycle; obtaining a set of concentration measurements of one or more peptide hormones of the TGF-β superfamily in such samples over such two or more days; detecting an onset of a change in the relative magnitude or slope of successive concentration measurements of such one or more peptide hormones during such cycle; and coordinating live cover or artificial insemination of such mare to occur no later than three days after such onset. The disclosed method may be performed using an associated system that measures, processes and stores the measurements. The system may detect and optionally provide notification of the onset of such change.
Velvet disease infestation is detected using rmAbs that are cross-reactive with one or more A. ocellatum or P. pillulare antigens. The analysis may be performed shipboard, dockside, in an aquaculture or aquarium setting, otherwise in situ at the point of sample collection or elsewhere. The results may be used to monitor health and disease of captured or cultured fish species or the safety of water to be introduced into an aquaculture facility.
Brevetoxin (BTX) concentration levels in organisms and other samples are detected using conjugated recombinant monoclonal brevetoxin antibodies (BTX-rAbs) that are cross-reactive with one or more brevetoxin antigens. The analysis may employ a simplified and less pure, but more easily, more safely or more rapidly prepared crude tissue extract. The results may be used to permit or not permit the harvesting, sale, relocation or depuration of such organisms or samples.
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, ocean acidification, benthic ecology, shark biology and conservation, behavioral ecology and physiology, sensory biology and behavior, coral reef ecology, and microbiology Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection; entertainment and educational services in the nature of animal exhibitions and providing aquarium facilities to the public Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
12.
BIOMARKERS FOR PREDICTING IMMINENT OVULATION 0R REPRODUCTIVE COMPETENCE IN MARES
Broodmare imminence of ovulation and reproductive competence may be determined by collecting blood, serum or plasma samples from a mare over two or more days in such mare's estrous cycle; obtaining a set of concentration measurements of one or more peptide hormones of the TGF-β superfamily in such samples over such two or more days; detecting an onset of a change in the relative magnitude or slope of successive concentration measurements of such one or more peptide hormones during such cycle; and coordinating live cover or artificial insemination of such mare to occur no later than three days after such onset. The disclosed method may be performed using an associated system that measures, processes and stores the measurements. The system may detect and optionally provide notification of the onset of such change.
Fish sexual characteristics are determined by measuring the concentration in fish serum, plasma or whole blood of one or more peptide hormones of the transforming growth factor-beta superfamily (TGF-β superfamily). The disclosed method and an accompanying field test kit may be used in sturgeon aquaculture to cull out young male fish so that increased time and resources may be devoted to the further rearing of female fish for caviar production. The method and test kit may also be used for wild fish life history studies.
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
41 - Education, entertainment, sporting and cultural services
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
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Education services, namely, providing classes, seminars, and workshops for all ages in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection Conduct scientific research programs in the fields of conservation, the sustainable use of marine biodiversity, healthy habitats and natural resources, behavioral ecology and physiology, ecotoxicology, chemical and physical ecology, phytoplankton ecology, ocean technology, marine immunology, environmental health, fisheries habitat ecology, marine stock enhancement, coral reef restoration, marine and freshwater aquaculture research, ocean acidification, marine biomedical research, benthic ecology, sea turtle conservation and research, spotted eagle ray conservation research, shark biology and conservation, coral reef monitoring and assessment, behavioral ecology and physiology, dolphin research, manatee research, stranding investigations, sensory biology and behavior, coral reef ecology, microbiology and data collection
19.
Fish sexual characteristic determination using peptide hormones
Fish sexual characteristics are determined by measuring the concentration in fish serum, plasma or whole blood of one or more peptide hormones of the transforming growth factor-beta superfamily (TGF-β superfamily). The disclosed method and an accompanying field test kit may be used in sturgeon aquaculture to cull out young male fish so that increased time and resources may be devoted to the further rearing of female fish for caviar production. The method and test kit may also be used for wild fish life history studies.
Fish sexual characteristics are determined by measuring the concentration in fish serum, plasma or whole blood of one or more peptide hormones of the transforming growth factor-beta superfamily (TGF-β superfamily). The disclosed method and an accompanying field test kit may be used in sturgeon aquaculture to cull out young male fish so that increased time and resources may be devoted to the further rearing of female fish for caviar production. The method and test kit may also be used for wild fish life history studies.
An apparatus and method for making quantitative measurements of the amounts of optical brighteners in water samples using fluorescence measurements at multiple wavelengths. First and second emission wavelength raw measurements are corrected for sample absorption to provide absorption-corrected first emission wavelength and second emission wavelength fluorescence emission values. The absorption-corrected first emission wavelength value is compared to the absorption-corrected second emission wavelength value and to similarly-determined and similarly absorption-corrected emission wavelength values or a ratio thereof obtained from a comparison water sample in which optical brighteners are not present or are only minimally present, to provide a quantitative optical brightener measurement. The apparatus and method may be field-based or laboratory-based, and may operate on a flow-through basis or on discrete samples.
