Abstract

Provided is a method which comprises developing a high-expression promoter of Pseudozyma antarctica, constructing a heterologous protein production system with the use of the obtained high-expression promoter, and then efficiently producing an enzyme using a strain belonging to the order Ustilaginales including a yeast of the genus Pseudozyma. The heterologous protein production method according to the present invention comprises: a step for culturing host cells, which have been transformed with an expression vector that contains a base sequence represented by SEQ ID NO:1 or SEQ ID NO:7 and a base sequence, said base sequence encoding a heterologous protein, functionally bound thereto, under such conditions as allowing the expression of the heterologous protein; and a step for harvesting the heterologous protein from a liquid culture medium.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

Provided are: a transporter gene involved in the promotion or inhibition of the absorption of Cd by a root; a mutant gene of the transporter gene; a transporter protein of the transporter gene; a rice plant of which the absorption of Cd is reduced; and a method for selecting and growing a rice plant of which the absorption of Cd is reduced. A gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 2; a gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 3; a gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 4; and a transporter protein involved in the regulation of the absorption of cadmium, which comprises an amino acid sequence represented by SEQ ID NO: 1.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01H 1/00 - Processes for modifying genotypes
• A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• G01N 33/15 - Medicinal preparations
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing

Abstract

A multifunctional formed object containing an adsorbent for hazardous substances is provided in the form of a water-soluble sheet or tablet. The formed object comprises a hazardous-substance-adsorbing formed object having a multiple function imparted thereto, and can be applied to a contaminated environment while preventing a fine powder from flying off or causing dust explosion and without necessitating much labor. After application, the formed object adsorptively fixes thereto a hazardous substance present in the contaminated environment. The hazardous-substance-adsorbing formed object, to which a hazardous substance is adsorptively fixed in a contaminated environment, e.g., a soil, is produced from a plurality of raw-material ingredients, one of which is a powdery adsorbent for hazardous substances. The adsorbent is a porous fine powder which includes particles each having a diameter capable of passing through 200-mesh and a particle density of an average particle size of 100 µm or smaller, the proportion of the particles being more than 80% of the weight of the powder. Besides the adsorbent, an additive for imparting another function is suitably incorporated.

IPC Classes  ?

• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
• A01C 21/00 - Methods of fertilising
• B01J 20/12 - Naturally occurring clays or bleaching earth
• B01J 20/14 - Diatomaceous earth
• B01J 20/18 - Synthetic zeolitic molecular sieves
• B01J 20/20 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising free carbonSolid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising carbon obtained by carbonising processes
• B09C 1/02 - Extraction using liquids, e.g. washing, leaching
• B09C 1/08 - Reclamation of contaminated soil chemically
• B09C 1/10 - Reclamation of contaminated soil microbiologically or by using enzymes
• D21H 21/14 - Non-fibrous material added to the pulp, characterised by its function, form or propertiesPaper impregnating or coating material, characterised by its function, form or properties characterised by function or properties in or on the paper
• A01N 25/34 - Shaped forms, e.g. sheets, not provided for in any other group of this main group
• A01N 29/04 - Halogen directly attached to a carbocyclic ring system
• A01N 29/06 - Hexachlorocyclohexane
• A01N 29/08 - Halogen directly attached to a polycyclic ring system
• A01N 29/12 - 1,1-Di- or 1,1,1-tri-halo-2-aryl-ethane or -ethene or derivatives thereof, e.g. DDT
• A01N 31/08 - Oxygen or sulfur directly attached to an aromatic ring system
• A01P 3/00 - Fungicides
• A01P 5/00 - Nematocides
• A01P 7/02 - Acaricides
• A01P 7/04 - Insecticides
• C05G 5/00 - Fertilisers characterised by their form

Abstract

Disclosed are: a novel microorganism which can decompose a methylthiotriazine compound (particularly, simetryn, dimethametryn, prometryn), a chlorotriazine compound (particularly, simazine, atrazine, propazine) and a methoxytriazine compound (particularly, simeton, atraton, prometon) which have been frequently used as agrichemicals or the like; and a method for decomposing a methylthiotriazine compound, a chlorotriazine compound and/or a methoxytriazine compound by using the microorganism. Specifically disclosed are: a novel bacterium Nocardioides sp. strain MTD22 which is capable of decomposing a methylthiotriazine compound, a chlorotriazine compound and a methoxytriazine compound; and a method for decomposing a methylthiotriazine compound, a chlorotriazine compound and/or a methoxytriazine compound, particularly simetryn, dimethametryn, prometryn, simazine, atrazine, propazine, simeton, atraton and/or prometon, by using the microorganism.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• A62D 3/02 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
• B09C 1/10 - Reclamation of contaminated soil microbiologically or by using enzymes
• C02F 3/00 - Biological treatment of water, waste water, or sewage
• A62D 101/04 - Pesticides, e.g. insecticides, herbicides, fungicides or nematicides
• A62D 101/22 - Organic substances containing halogen
• A62D 101/26 - Organic substances containing nitrogen or phosphorus
• A62D 101/28 - Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen

Abstract

Disclosed are: a microorganism capable of degrading diphenylarsinic acid; a method for degrading diphenylarsinic acid by using the microorganism; a method for clean-up of a contaminated soil by using the microorganism; an agent for degrading diphenylarsinic acid, which comprises the microorganism; and a cleaning agent for a contaminated soil or groundwater, which comprises the microorganism. Specifically disclosed are: a microorganism belonging to the genus Sinorhizobium and capable of degrading diphenylarsinic acid; a microorganism belonging to the genus Ensifer and capable of degrading diphenylarsinic acid; a method for clean-up of a contaminated soil by using the microorganism; an agent for degrading diphenylarsinic acid, which comprises the microorganism; and a cleaning agent for a contaminated soil or groundwater, which comprises the microorganism.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• A62D 3/00 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances
• C07F 9/74 - Aromatic compounds
• C12N 15/09 - Recombinant DNA-technology

Abstract

A method by which a heavy metal contained in a paddy-field soil is efficiently removed to clean the soil. The method of treating a paddy-field soil containing a heavy metal is an in-situ cleaning method which comprises washing the heavy-metal-containing paddy-field soil with an aqueous solution of a chemical and then washing it with water. It is characterized in that the washing is conducted while adding the aqueous chemical solution or water so that the depth of the furrow pans from the water surface is 35 cm or more.

IPC Classes  ?

• B09C 1/02 - Extraction using liquids, e.g. washing, leaching
• A01B 33/02 - Tilling implements with rotary driven tools with tools on horizontal shaft transverse to direction of travel
• A01B 33/08 - ToolsDetails, e.g. adaptations of transmissions or gearings
• B09B 3/00 - Destroying solid waste or transforming solid waste into something useful or harmless
• B09C 1/08 - Reclamation of contaminated soil chemically
• C02F 1/62 - Heavy metal compounds
• C02F 11/14 - Treatment of sludgeDevices therefor by de-watering, drying or thickening with addition of chemical agents

Abstract

A method for the reductive disinfection of soil which comprises incorporating a dilute aqueous solution of ethanol into soil to form a watered soil and keeping the watered soil intact while inhibiting the evaporation of water and ethanol to disinfect the soil; a reductive disinfectant for soil which consists of an aqueous solution of ethanol; a method for the wetting disinfection of soil which comprises incorporating a dilute aqueous solution of ethanol into soil to wet the soil and keeping the wetted soil intact while inhibiting the evaporation of water and ethanol to disinfect the soli; a wetting disinfectant for soil which consists of an aqueous solution of ethanol; a system for drenching soil with a disinfectant which is provided with a water supply pipe, one end of which is connected to a source of water supply and the other end of which is connected to the inlet of a watering tube, a liquid manure feeder, a storage tank for aqueous ethanol solution, and a watering tube having many branches; and a method for the reductive disinfection of soil, a reductive disinfectant for soil, a method for the wetting disinfection of soli, a wetting disinfectant for soil, and a system for drenching soil with a disinfectant which are each as described above, wherein acetic acid is simultaneously used.

IPC Classes  ?

• A01N 31/02 - Acyclic compounds
• A01M 17/00 - Apparatus for the destruction of vermin in soil or in foodstuffs
• A01N 25/02 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
• A01N 37/02 - Saturated carboxylic acids or thio-analogues thereofDerivatives thereof
• A01P 3/00 - Fungicides
• A01P 5/00 - Nematocides
• A01P 13/00 - HerbicidesAlgicides