Abstract

The evaluation system for evaluating a status of rumen fermentation in a ruminant includes an evaluation device that has an estimation unit for estimating a status of rumen fermentation in a ruminant based on a milk production record which indicates a milk yield and a milk component of milk produced by the ruminant. According to the evaluation system, it is possible to easily evaluate the status of rumen fermentation in the ruminant.

IPC Classes  ?

• A01J 5/01 - MilkmetersMilk flow sensing devices
• A01K 29/00 - Other apparatus for animal husbandry
• A61B 5/00 - Measuring for diagnostic purposes Identification of persons

Abstract

A calving difficulty estimating device (100) is for estimating calving difficulties. The calving difficulty estimating device (100) comprises: an acquisition unit (11) for acquiring a captured image that includes a subject cow; an extraction unit (12) for extracting the cow image that is the subject cow image from the captured image; a generation unit (13) for referring to the cow image and generating skeletal information representing the feature points of the subject cow; and an estimation unit (14) for referring to the skeletal information and estimating calving difficulties in the subject cow.

IPC Classes  ?

• A01K 29/00 - Other apparatus for animal husbandry

Abstract

This evaluation system for evaluating the state of rumen fermentation in a ruminant comprises an evaluation device having an estimation unit for estimating the state of rumen fermentation in a ruminant on the basis of milk production results representing the amount and components of milk produced by the ruminant. This evaluation system makes it possible to simply evaluate the state of rumen fermentation in a ruminant.

IPC Classes  ?

• G01N 33/04 - Dairy products
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• A01K 29/00 - Other apparatus for animal husbandry

Abstract

2n+1 and n is an integer of 1 or more but 15 or less).

IPC Classes  ?

• A01N 37/36 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio-analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio-analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
• A01M 17/00 - Apparatus for the destruction of vermin in soil or in foodstuffs
• A01N 25/02 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
• A01N 25/12 - Powders or granules
• A01N 61/00 - Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action

Abstract

a step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

In order to identify a novel gene responsible for parthenocarpy and to provide use of the gene, the parthenocarpy regulatory gene of the present invention includes a polynucleotide (1) that encodes an amino acid sequence represented by SEQ ID NO: 1, (2) that encodes an amino acid sequence (i) having a sequence identity of 75% or higher relative to the amino acid sequence represented by SEQ ID NO: 1, (3) that encodes an amino acid sequence in which 1 to 98 amino acids are substituted, etc. in the amino acid sequence represented by SEQ ID NO: 1, or (4) that is hybridized with a polynucleotide, which has a sequence complementary to the polynucleotide for encoding the amino acid sequence represented by SEQ ID NO: 1 under a stringent condition.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C12N 9/10 - Transferases (2.)
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

It has been found that a marker gene, which is inserted in the genomic DNA via homologous recombination, and to both ends of which nuclease recognition sites are added, can be removed from a plant cell by using a corresponding nuclease, and further that the nuclease recognition sites can also be removed without leaving any trace by matching sequences of at least 30 nucleotides adjacent to the recognition sites. Moreover, in a method for introducing a mutation into a target DNA on the genome of a plant cell via homologous recombination, it is made possible to: stably select a plant cell, in which the mutation is introduced, based on an expression of a marker gene; further, to remove an unnecessary sequence such as the marker gene from the selected cell; and to introduce only a required mutation into the target DNA.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

The purpose of the present invention is to elucidate the genetic information of an Rsv4 gene having potyvirus resistance and to develop a use for the same. The present invention provides a protein with which it is possible to impart potyvirus resistance to plants, a polynucleotide encoding this protein, a vector and transformants having this polynucleotide, a method for producing transformed plants to which potyvirus resistance has been imparted, a primer set for detecting potyvirus resistance, a kit for detecting potyvirus resistance, and a method for assessing the potyvirus resistance of soybeans.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01H 1/00 - Processes for modifying genotypes
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 9/22 - Ribonucleases

Abstract

Provided is a soil-borne disease control method whereby it becomes possible to control a soil-pathogen in a simple manner. A soil-borne disease control method characterized by applying at least one polyhydroxyalkanoic acid having a structure represented by formula (1) onto soil. [-CHR-CH2-CO-O-] (1) (In the formula, R represents an alkyl group represented by CnH2n+1; and n represents an integer of 1 to 15 inclusive.)

IPC Classes  ?

• A01N 37/36 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio-analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio-analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
• A01M 17/00 - Apparatus for the destruction of vermin in soil or in foodstuffs
• A01N 25/00 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests
• A01N 61/00 - Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
• A01P 3/00 - Fungicides

Abstract

Provided is a technique with which it is possible to produce a glucoraphasatin-deficient daikon line in a short time at high accuracy, as pertains to a technique for fundamentally overcoming the problems of deterioration of flavor, occurrence of radish odor, and deterioration of quality such as yellowing in processed daikon foods and the like. The present invention pertains to an inoperative-type glucoraphasatin synthase gene having (A) the characteristic of having a mutation associated with complete or partial deletion of the 2-oxoglutarate-iron(II)-dependent oxygenase domain in an encoded protein within an exon constituting a glucoraphasatin synthase gene, and (B) the characteristic of having the glucoraphasatin of the root portion deleted or substantially deleted when the glucoraphasatin synthase gene locus in the daikon genome is a homo-type of this inoperative-type gene.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01H 1/00 - Processes for modifying genotypes
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 5/02 - Flowers
• A01H 5/04 - Stems
• A01H 5/06 - Roots
• A01H 5/10 - Seeds
• A01H 5/12 - Leaves
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

The purpose of the present invention is to provide an anthocyanin-based pigment composition having high quality which has not been attained hitherto, at a higher efficiency and lower cost than a red cabbage pigment. This anthocyanin-based pigment composition is derived from a plant other than red cabbage and has the following features (A), (B), and (C): (A) an aqueous solution which contains the anthocyanin-based pigment composition and has a pH adjusted to 3 with a citric acid buffer (pH, 3.0) has a maximum absorption wavelength in the visible-light region of 500-550 nm; (B) when a pigment-composition-containing aqueous solution comprising the anthocyanin-based pigment composition, 0.2 mass% citric acid, 20 mass% ethanol, and water is prepared so as to have a color value E10%1cm of 10 and poured in an amount of 1 mL into a 20-mL vial and this vial is closed, stored at 50°C for 5 days, and thereafter held at 40°C for 30 minutes, then the total amount of methyl mercaptan, dimethyl disulfide, and dimethyl trisulfide contained in the gas present in the closed space of the vial is 100 pg/mL or less; and (C) the gas present in the closed space of the vial which has undergone the 40°C 30-minute holding in (B) above contains 5-methylthiopentanenitrile.

IPC Classes  ?

• C09B 67/44 - Solutions
• A23L 1/27 - Colouring or decolouring of foods
• A61K 8/60 - SugarsDerivatives thereof
• A61K 8/97 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from algae, fungi, lichens or plantsCosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from derivatives thereof
• A61K 47/26 - Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharidesDerivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• A61K 47/46 - Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
• C09B 61/00 - Dyes of natural origin prepared from natural sources

Abstract

It was discovered that when a combination consisting of a protein secreted by a bacterium belonging to the genus Salmonella with dead cells of a bacterium belonging to the genus Salmonella was administered to a living organism, a highly excellent defense effect against the infection with a bacterium belonging to the genus Salmonella was achieved compared with the case of administering either the protein or the dead cells singly. Thus, provided is a vaccine that has a high safety and an excellent defense capability against salmonellosis.

IPC Classes  ?

• A61K 39/112 - SalmonellaShigella
• A61K 39/116 - Polyvalent bacterial antigens
• A61P 31/04 - Antibacterial agents
• A61P 37/04 - Immunostimulants

Abstract

The present invention provides a genetic modification method for poultry primordial germ cells, characterized by modifying a gene of the poultry primordial germ cell by genome editing.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01K 67/027 - New or modified breeds of vertebrates
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

　In order to provide an antibody or antibody fragment including the variable region of said antibody for the H5 subtypes of the avian influenza virus, an antigen polypeptide, and uses thereof, the present invention provides: an antibody or antibody fragment including the variable region of said antibody, which specifically binds to the HA1 antigen polypeptide of the HA1 region of the H5 subtypes of the avian influenza virus, said antigen polypeptide comprising the amino acid sequence represented by SEQ ID NO: 10; the HA1 antigen polypeptide comprising the amino acid sequence represented by SEQ ID NO: 10; and uses of the antibody and antibody fragment including the variable region of the antibody.

IPC Classes  ?

• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• C07K 16/46 - Hybrid immunoglobulins
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 15/09 - Recombinant DNA-technology
• C12P 21/08 - Monoclonal antibodies
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

Provided is a method for producing a standard sample having a target sequence the same as a DNA fragment having a specific number of copies of the target sequence. After the DNA fragment having the specific number of copies of the target sequence is introduced into cells, the cells are cultured, and the cultured cells are isolated. Thus, a standard sample having a target sequence identical to the DNA fragment having a specific number of copies of the target sequence is produced.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms

Abstract

　It was discovered that, in a plant cell, a marker gene inserted into the genomic DNA by homologous recombination and having nuclease recognition sites added to both ends thereof can be removed by the corresponding nuclease, and the recognition sites can be removed without leaving a trace by matching the sequence of at least 30 nucleotides adjacent to the nuclease recognition sites. Thus, it is possible, in a method for introducing a mutation into target DNA on a plant cell genome by homologous recombination, to select, in a stable manner and as an indicator of expression of the marker gene, plant cells into which this mutation has been introduced, and it is possible to remove unnecessary sequences such as marker genes and to introduce only the necessary mutation into the target DNA in the selected cells.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01H 1/00 - Processes for modifying genotypes
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

In order to provide a detection method and kit capable of quickly and accurately detecting two or more target organisms simultaneously, the present invention provides a method for simultaneously detecting two or more target organisms which involves a ligase chain reaction step in which two or more types of oligomer sets, which correspond to each of the two or more target organisms, are used simultaneously to perform ligase chain reactions with the detection target samples as templates, and a detection step in which the presence or absence of amplified DNA fragments derived from each of the oligomer sets from the ligase chain reaction is detected, wherein the oligomer sets are configured from four oligomers for a ligase chain reaction that are designed to comprise therewithin any single nucleotide polymorphism (SNP) of the corresponding detection target organism.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

This method for producing a plant cell to which a mutation has been introduced to target DNA includes: a step for introducing into a plant cell a DNA construct that contains DNA homologous to the target DNA and in which a PiggyBac transposon containing a marker gene has been introduced to the homologous DNA and a desired mutation has been introduced; a step for selecting, with the expression of the marker gene as an indicator, plant cells to which the target DNA has been introduced by means of homologous recombination of the mutation and the PiggyBac transposon; and a step for eliminating the PiggyBac transposon from the target DNA by means of constitutive expression of the PiggyBac transposon in the cells selected in the aforementioned step.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

[Problem] To identify a novel gene that participates in parthenocarpy, and to provide a use of this gene. [Solution] This parthenocarpy regulation gene contains a polynucleotide that (1) encodes the amino acid sequence of SEQ ID NO: 1, (2) encodes an amino acid sequence having 75% or higher sequence identity with the amino acid sequence of SEQ ID NO: 1, (3) encodes an amino acid sequence obtained by substituting, etc., 1-98 amino acids in the amino acid sequence of SEQ ID NO: 1, or (4) hybridizes with a polynucleotide comprising a sequence complementary to a polynucleotide encoding the amino acid sequence of SEQ ID NO: 1 under stringent conditions.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

The purpose of the present invention is to provide: an acid-fast bacterium proliferation promoter capable of rapidly and markedly promoting the proliferation of an acid-fast bacterium; an acid-fast bacterium culturing method capable of rapidly growing an acid-fast bacterium in large quantities; and an acid-fast bacterium culture medium capable of rapidly and markedly promoting the proliferation of an acid-fast bacterium. The present invention provides: an acid-fast bacterium proliferation promoter containing bovine RegIIIγ lectin as an active ingredient; an acid-fast bacterium culturing method characterized in that the bacterium is cultured by adding said proliferation promoter to the medium; and an acid-fast bacterium culture medium containing said proliferation promoter.

IPC Classes  ?

• C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
• C12N 1/20 - BacteriaCulture media therefor

Abstract

The purpose of the present invention is to inexpensively provide a photocatalyst, which is a photocatalyst that can be used in organic material decomposition or sterilization, is not limited in utilization situations, and is highly safe, and which exhibits activity by absorbing light of a wide range of wavelengths including visible light. Provided are: a photocatalyst formed by mixing reducing organic compound having iron reducing capability and an iron supplying raw material in the presence of water and the reaction product obtained as the active ingredient, specifically, a photocatalyst exhibiting photocatalytic activity when irradiated with light of wavelengths among ultraviolet rays, visible light, or infrared light; an organic material decomposition method characterized by bringing the photocatalyst and material to be decomposed into contact and irradiating the same with light of wavelengths among ultraviolet rays, visible light, and infrared rays; and a sterilization method characterized by bringing the photocatalyst and material to be sterilized into contact and irradiating the same with light of wavelengths among ultraviolet rays, visible light, or infrared rays.

IPC Classes  ?

• B01J 35/02 - Solids
• A01N 55/02 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms
• A01P 1/00 - DisinfectantsAntimicrobial compounds or mixtures thereof
• A61L 2/08 - Radiation
• A61L 2/16 - Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lensesAccessories therefor using chemical substances
• B01D 53/86 - Catalytic processes
• B01J 31/04 - Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides containing carboxylic acids or their salts
• B01J 37/03 - PrecipitationCo-precipitation
• B01J 37/04 - Mixing

Abstract

The present invention pertains to a reinforcement structure for an embankment such as a dam, and a method for constructing the same. A square-shaped concrete block (4) is configured by forming an inclined surface (6A) on the outer side, burying one end of a tension-reinforcing member (5) in the interior, and extending the other end from a rear side (4i) to the outside. During construction, blocks (4) are connected one to another in a crosswise direction to form a row (4A), and an embankment material (9) is introduced inside and compacted. This is repeated to stack up a sequential row (4B). Damage such as slipping of the embankment and sliding, tearing-off, collapse, etc., of a covering block, as caused by the force of an earthquake or a tsunami collision, a fluid force due to a tsunami overflow, and scouring of a rear foundation, can be substantially prevented; even if damage is suffered, a chain-reaction of collapse can be avoided.

IPC Classes  ?

• E02B 3/12 - Revetment of banks, dams, watercourses, or the like
• E02B 3/14 - Preformed blocksArrangements thereof
• E02D 17/18 - Making embankments