Abstract

Provided herein are systems, methods, and cryoprotective solutions for reversible cryopreservation of biological specimens, whole organs, and whole organisms. Exemplary methods include loading a cryoprotective agent into the biological specimen, cooling the biological specimen to a cryogenic temperature for preservation, storing the biological specimen at a preservation temperature state to preserve the biological specimen, rewarming the biological specimen by increasing a temperature of the biological specimen above the preservation temperature state, and unloading the cryoprotective agent from the biological specimen. The cooling is performed at a first rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking of the preserved biological specimen, and at a first pressure to prevent or reduce ice expansion within the preserved biological specimen. The rewarming is performed at a second rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking, and at a second pressure to prevent or reduce ice expansion.

IPC Classes  ?

• A01N 1/125 - Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
• A01N 1/143 - Apparatus for organ perfusion
• A01N 1/162 - Temperature processes, e.g. following predefined temperature changes over time
• A01N 1/168 - Physical preservation processes using electromagnetic fields or radiationPhysical preservation processes using acoustic waves or corpuscular radiation

Abstract

Provided herein are systems, methods, and cryoprotective solutions for reversible cryopreservation of biological specimens, whole organs, and whole organisms. Exemplary methods include loading a cryoprotective agent into the biological specimen, cooling the biological specimen to a cryogenic temperature for preservation, storing the biological specimen at a preservation temperature state to preserve the biological specimen, rewarming the biological specimen by increasing a temperature of the biological specimen above the preservation temperature state, and unloading the cryoprotective agent from the biological specimen. The cooling is performed at a first rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking of the preserved biological specimen, and at a first pressure to prevent or reduce ice expansion within the preserved biological specimen. The rewarming is performed at a second rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking, and at a second pressure to prevent or reduce ice expansion.

IPC Classes  ?

• A01N 1/16 - Physical preservation processes
• A01N 1/162 - Temperature processes, e.g. following predefined temperature changes over time
• A01N 1/165 - Pressure processes, e.g. following predefined pressure changes over time
• A01N 1/125 - Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
• A01N 1/14 - Mechanical aspects of preservationApparatus or containers therefor

Abstract

Provided herein are systems, methods, and cryoprotective solutions for reversible cryopreservation of biological specimens, whole organs, and whole organisms. Exemplary methods include loading a cryoprotective agent into the biological specimen, cooling the biological specimen to a cryogenic temperature for preservation, storing the biological specimen at a preservation temperature state to preserve the biological specimen, rewarming the biological specimen by increasing a temperature of the biological specimen above the preservation temperature state, and unloading the cryoprotective agent from the biological specimen. The cooling is performed at a first rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking of the preserved biological specimen, and at a first pressure to prevent or reduce ice expansion within the preserved biological specimen. The rewarming is performed at a second rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking, and at a second pressure to prevent or reduce ice expansion.

IPC Classes  ?

• A01N 1/125 - Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
• A01N 1/143 - Apparatus for organ perfusion
• A01N 1/162 - Temperature processes, e.g. following predefined temperature changes over time
• A01N 1/168 - Physical preservation processes using electromagnetic fields or radiationPhysical preservation processes using acoustic waves or corpuscular radiation

Goods & Services

Cryopreservation of human tissue; Cryopreservation services; Cryopreservation of animal genetic resources

Goods & Services

Biomedical research services; Research and development of technology in the field of cryopreservation and reanimation Licensing of technology in the field of cryopreservation and reanimation, namely, licensing of intellectual property

Goods & Services

Cryopreservation of human tissue; Cryopreservation services; Cryopreservation of animal genetic resources

Goods & Services

Biomedical research services; Research and development of technology in the field of cryopreservation and reanimation Licensing of technology in the field of cryopreservation and reanimation, namely, licensing of intellectual property

Goods & Services

Cryopreservation of human tissue; Cryopreservation services; Cryopreservation of animal genetic resources

Goods & Services

Biomedical research services; Research and development of technology in the field of cryopreservation and reanimation Licensing of technology in the field of cryopreservation and reanimation, namely, licensing of intellectual property

Goods & Services

Cryopreservation of human tissue; Cryopreservation services; Cryopreservation of animal genetic resources

Goods & Services

Biomedical research services; Research and development of technology in the field of cryopreservation and reanimation Licensing of technology in the field of cryopreservation and reanimation, namely, licensing of intellectual property