Abstract

A quality control method (100) for determining run errors for an analysis-method (10) for quantifying a plurality of analytes in a plurality of biological samples.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G16B 99/00 - Subject matter not provided for in other groups of this subclass

Abstract

The present invention relates to quality control (QC) of data produced from a protein detection system and in particular to methods, components and software for detecting systematic errors in a protein detection system. The methods comprise receiving (S502) an amount of the protein in each sample; determining (S504) a pattern of the array for the protein by thresholding the determined amount for each sample, such that the determined amount of the protein for each sample is represented by one of a plurality of predetermined discrete values in the pattern; comparing (S506) the pattern with a plurality of predetermined patterns, each predetermined pattern corresponding to a systematic error in the protein detection system, and determining a similarity value for each of the predetermined patterns; upon the similarity value exceeding (S508) a pattern specific similarity threshold, indicating (S510) the systematic error in a data structure of candidate systematic errors.

IPC Classes  ?

• G16B 25/00 - ICT specially adapted for hybridisationICT specially adapted for gene or protein expression
• G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding

Abstract

The present invention relates to a device comprising at least one reaction space with a single pre-dispensed dried reagent composition suitable for performing an assay method for detecting at least one analyte in a sample to be added to the reaction space, wherein the reagent composition comprises at least one set of analyte-specific proximity probes and a drying stabiliser, wherein the proximity probes in the set each comprise a binding domain for binding directly or indirectly to the analyte and a nucleic acid domain. The invention further relates to a method of performing a detection assay with use of the device, as well as a method of preparing the device.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• G01N 33/536 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase

Abstract

Disclosed herein is a method of building a grid for presenting associations between protein expression levels and study variables of interest. The method includes building a grid frame including different sections representing different biological pathways, assigning individual proteins to specific locations as points in the sections of the grid frame corresponding to individual proteins' function in the biological pathways, collecting detected expression levels of the individual proteins, and adjusting appearance of the points in the grid frame based on the expression levels of the individual proteins.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The present invention relates to a library comprising a plurality of detection probes suitable for detection of a set of analytes of interest, each detection probe comprising an analyte-specific binding domain and a nucleic acid moiety, the nucleic acid moiety of a detection probe being capable of generating a reporter nucleic acid molecule comprising an identification sequence identifying the respective analyte of interest, said library being wherein the library comprises, for one or more analytes of interest, a set of two or more detection probes wherein each detection probe within the set is capable of generating a reporter nucleic acid molecule comprising an identification sequence that differ from the identification sequences generated by the other members of the same set. The invention further relates to a method for composing a panel with use of the library, as well as a computer program implementing said method.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens

Abstract

A method for detecting a plurality of analytes in a sample comprises performing a multiplex proximity-based detection assay. The assay utilises pairs of proximity probes with shared hybridisation sites (i.e. hybridisation sites which are shared between different proximity probe pairs). A product comprising a plurality of proximity probe pairs with shared hybridisation sites may be used in the method disclosed herein.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens

Goods & Services

Apparatus, instruments and software for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms; recorded and downloadable software and media being presentations of data related to protein expression levels in biological samples or living organisms. Apparatus and instruments for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms, for use in diagnosis and medical use; diagnostic apparatus for medical use with recorded and downloadable software and media being presentations of data related to protein expression levels in biological samples or living organisms, for diagnosis and medical use. Processing and analysing data related to protein expression levels in biological samples or living organisms; providing non-downloadable computer software for processing, analysis and presentation of data related to protein expression levels in biological samples or living organisms. Remote monitoring of medical data for medical diagnosis and treatment, namely, processing, analysis and presentation of data relating to protein expression levels in biological samples or living organisms for medical and diagnostic use; medical analysis for diagnosing and treatment of patients; performing diagnosis of diseases and medical conditions.

Abstract

The present invention provides a method of detecting multiple analytes in a sample, wherein said analytes have varying levels of abundance in the sample, said method comprising: (i) providing multiple aliquots from the sample; and (ii) in each aliquot, detecting a different subset of the analytes by performing a separate multiplex assay for each aliquot, wherein the analytes in each subset are selected based on their predicted abundance in the sample.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Goods & Services

(1) Laboratory apparatus and instruments for processing, analyzing and presenting data related to protein expression levels in biological samples and living organisms for research use, and software for processing, analyzing and presenting data related to protein expression levels in biological samples and living organisms; recorded and downloadable software for processing, analyzing and presenting data related to protein expression levels in biological samples and living organisms. (2) Medical apparatus and instruments for processing, analyzing and presenting data related to protein expression levels in biological samples and living organisms, for use in diagnosis and medical use; medical diagnostic apparatus for medical use with recorded and downloadable software for processing, analyzing and presenting data related to protein expression levels in biological samples and living organisms, for diagnosis and medical use. (1) Processing and analysing data related to protein expression levels in biological samples and living organisms; providing non-downloadable computer software for processing, analysis and presentation of data related to protein expression levels in biological samples and living organisms. (2) Remote monitoring of medical data for medical diagnosis and treatment, namely, processing, analysis and presentation of data relating to protein expression levels in biological samples and living organisms for medical and diagnostic use; medical analysis for diagnosing and treatment of patients; performing diagnosis of diseases and medical conditions.

Goods & Services

Scientific apparatus and instruments, namely, apparatuses and instruments for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms; Downloadable software for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms; Recorded and downloadable software for presenting data related to protein expression levels in biological samples or living organisms Medical apparatus and instruments for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms, all for use in diagnosis and medical use; diagnostic apparatus for medical use for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms incorporating recorded and downloadable software for presenting data related to protein expression levels in biological samples or living organisms; all for diagnosis and medical use Analyzing data related to protein expression levels in biological samples or living organisms, namely, protein testing of biological samples or living organisms for scientific research purposes; Providing online non-downloadable computer software for processing, analysis and presentation of data related to protein expression levels in biological samples or living organisms Remote monitoring of medical data for medical diagnosis and treatment, namely, monitoring data relating to protein expression levels in biological samples or living organisms for medical and diagnostic use; medical analysis for diagnosing and treatment of patients; performing diagnosis of diseases and medical conditions

Goods & Services

Apparatus, instruments and computer software, recorded and downloadable, for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms; Data sets, recorded and downloadable, related to protein expression levels in biological samples or living organisms; software and media, being presentations of data related to protein expression levels in biological samples or living organisms.. Digital library services related to proteomics.

Goods & Services

Chemical compositions for use in scientific analysis of the composition and content of samples of biological and non-biological origin, other than for medical or veterinary purposes; Chemical compositions for use in scientific analysis, other than for medical or veterinary purposes, for use in measuring concentration of analytes in samples of biological origin, wherein the analytes are biomarkers, namely, peptides, proteins and nucleic acid molecules and combinations thereof Chemical preparations for medical purposes for measurement of analytes in samples of human and animal origin for diagnostic purposes, wherein the analytes are biomarkers, namely, peptides, proteins and nucleic acid molecules and combinations thereof; Chemical preparations for measurement of biomarker signatures in samples of human and animal origin for medical diagnostic purposes

Goods & Services

Chemical compositions for use in analysis of the composition and content of samples of biological and non-biological origin; Chemical compositions for use in measuring concentration of analytes in samples of biological origin, wherein the analytes are biomarkers such as peptides, proteins and nucleic acid molecules and combinations thereof. Chemical preparations for measurement of analytes in samples of human and animal origin for diagnostic purposes, wherein the analytes are biomarkers such as peptides, proteins and nucleic acid molecules and combinations thereof; Chemical preparations for measurement of biomarker signatures in samples of human and animal origin for diagnostic purposes.

Goods & Services

Chemical compositions for use in analysis of the composition and content of samples of biological and non-biological origin; Chemical compositions for use in measuring concentration of analytes in samples of biological origin, wherein the analytes are biomarkers such as peptides, proteins and nucleic acid molecules and combinations thereof. Chemical preparations for measurement of analytes in samples of human and animal origin for diagnostic purposes, wherein the analytes are biomarkers such as peptides, proteins and nucleic acid molecules and combinations thereof; Chemical preparations for measurement of biomarker signatures in samples of human and animal origin for diagnostic purposes.

Goods & Services

Apparatus, instruments and software for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms; Recorded and downloadable software and media being presentations of data related to protein expression levels in biological samples or living organisms. Apparatus, instruments and software for processing, analyzing and presenting data related to protein expression levels in biological samples or living organisms, for use in diagnosis and medical use; Recorded and downloadable software and media being presentations of data related to protein expression levels in biological samples or living organisms, for diagnosis and medical use. Processing, analysis and presentation of data related to protein expression levels in biological samples or living organisms. Processing, analysis and presentation of data related to protein expression levels in biological samples or living organisms for medical and diagnostic use; Medical analysis for diagnosing and treatment of patients; performing diagnosis of diseases and medical conditions.

Abstract

The present disclosure and invention provides a method of detecting DNA sequences from multiple pools. In the method, the pools are combined, DNA concatemers are generated by joining together a single DNA molecule from each pool in a pre-defined order, and the concatemers are then sequenced. By sequencing each concatemer, multiple DNA sequences are detected, and each DNA sequence detected can be assigned to its pool of origin by its location in the concatemer. The method thereby enables the specific detection of DNA sequences from each of multiple pools. A kit suitable for performing the method is also provided.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• C12Q 1/6804 - Nucleic acid analysis using immunogens

Abstract

Methods of detecting DNA sequences from multiple pools comprising at least one species of DNA molecule comprise combining the pools to form a combination pool; in the combination pool, generating at least one linear DNA concatemer containing one DNA molecule from each pool, wherein a position of each DNA molecule within the concatemer correlates to the pool from which the DNA molecule originated; and sequencing the concatemers, thereby detecting the DNA sequence of each DNA molecule at each position in each concatemer, wherein each detected DNA sequence is assigned to the pool from which its DNA molecule originated based upon its position within the concatemer.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6855 - Ligating adaptors
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The present invention provides a method of detecting multiple analytes in a sample, wherein said analytes have varying levels of abundance in the sample, said method comprising: (i) providing multiple aliquots from the sample; and (ii) in each aliquot, detecting a different subset of the analytes by performing a separate multiplex assay for each aliquot, wherein the analytes in each subset are selected based on their predicted abundance in the sample.

IPC Classes  ?

• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/6851 - Quantitative amplification
• C12Q 1/6804 - Nucleic acid analysis using immunogens

Abstract

The present invention provides a method of detecting an analyte in a sample, wherein the analyte is detected by detecting a reporter nucleic acid molecule specific for the analyte, said method comprising performing a PCR reaction to generate a PCR product of the reporter nucleic acid molecule and detecting said PCR product; wherein an internal control is provided for the PCR reaction, and said internal control is: (i) a separate component which is present in a pre-determined amount, and which is, or comprises, or leads to the generation of, a control nucleic acid molecule which is amplified by the same primers as the reporter nucleic acid molecules; and/or (ii) a unique molecular identifier (UMI) sequence present in each individual reporter nucleic acid molecule and/or each individual control nucleic acid molecule, which is unique to each molecule.

IPC Classes  ?

• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/6851 - Quantitative amplification

Abstract

The present invention provides a method for detecting a plurality of analytes in a sample, comprising performing a multiplex proximity-based detection assay. The assay utilises pairs of proximity probes with shared hybridisation sites (i.e. hybridisation sites which are shared between different proximity probe pairs). Also provided is a product comprising a plurality of proximity probe pairs with shared hybridisation sites, which may be used in the method disclosed herein.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens

Abstract

The present invention provides a method of detecting an analyte in a sample, wherein the analyte is detected by detecting a reporter nucleic acid molecule for the analyte, said method comprising performing a PCR reaction to generate a PCR product of the reporter nucleic acid molecule and detecting said PCR product, wherein an internal control is included in the PCR reaction and said internal control is present in a pre-determined amount and is, or comprises, or leads to the generation of, a control nucleic acid molecule, wherein the control nucleic acid molecule comprises a sequence which is the reverse sequence of the reporter nucleic acid molecule.

IPC Classes  ?

• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/6851 - Quantitative amplification

Goods & Services

Chemicals used in scientific analysis, research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical analysis kits including sample diluent, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers and control chemicals for research use and use in drug development. Chemicals for diagnostic use for medical or veterinary purposes. Apparatus and instruments for scientific and diagnostic use (other than for medical use); apparatus and instruments used in the detection of molecules, proteins and peptides (other than for medical use); diagnostic apparatus, instruments and software used in scientific analysis and detection of molecules for non-medical purposes; supplies for research use and drug development namely primer plates, containers and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. Laboratory research services regarding detection of molecules, proteins and peptides; drug development; diagnostic development; data science (biostatistics) services; laboratory biomarker analysis services and related data analysis.

Goods & Services

Chemicals used in scientific analysis, research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical analysis kits including sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers and control chemicals for research use and use in drug development. Chemicals for diagnostic use for medical or veterinary purposes. Apparatus and instruments for scientific and diagnostic use; apparatus and instruments used in the detection of molecules, molecular proteins and peptides; diagnostic apparatus, instruments and software used in scientific analysis and detection of molecules for non-medical purposes; kits for research use and drug development namely primer plates, containers and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. Research regarding detection of molecules, molecular proteins and peptides; drug development; diagnostic development; laboratory research services regarding detection of molecules, proteins and peptides; laboratory biomarker analysis services and related data analysis.

Goods & Services

Chemicals used in scientific analysis and research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical kits for analysing biological sample materials for scientific purposes comprising sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers in the nature of diagnostic preparations and chemical preparations serving as positive control chemicals reagents for research use and use in drug development Chemical preparations for biological sample analysis for medical diagnostic purposes Apparatus and instruments for scientific use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments; apparatus and instruments for diagnostic use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments for research use other than for medical use; scientific apparatus and instruments used in the measuring of molecules, molecular proteins and peptides other than for medical use; diagnostic apparatus and instruments used in scientific analysis, namely, diagnostic apparatus and instruments for the measurement of molecules and instruments for measuring proteins for non-medical purposes; downloadable software for measuring of molecules in the nature of molecule sequencing and data analysis; laboratory equipment and supplies for scientific research use and drug development, namely, primer PCR plates, containers and ampoules filled with incubation solutions, probe liquids being biochemical reagents, dilution and detection solutions as well as control liquids for use in testing and analyzing tissue and biological sample for non-medical purposes Laboratory research regarding detection of molecules, molecular proteins and peptides; pharmaceutical drug development services; diagnostic development, namely, custom design and development of chemical reagents and biochemical assays; data science and biostatistics services, namely, data analysis relating to protein biomarker analysis for scientific research purposes; laboratory biomarker analysis services and related data analysis

Goods & Services

(1) Chemicals used in scientific analysis, research and for industrial use, namely chemicals used in scientific analysis and detection of biomolecules, for determining the presence and quantity of a biomolecule in a sample including the steps of sample preparation and detection; biochemicals in the form of antibodies for research use; chemicals for detection of molecules, namely molecular proteins and peptides; chemical kits for analysing biological sample materials comprising sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers in the nature of diagnostic preparations and chemical preparations serving as control chemicals reagents for research use and use in drug development. (2) Chemicals for diagnostic use for medical or veterinary purposes, namely clinical diagnostic reagents; chemical preparations for biological sample analysis for medical diagnostic purposes. (3) Apparatus and instruments for scientific use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments other than for medical use; apparatus and instruments for diagnostic use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments for research use other than for medical use; apparatus and instruments, namely laboratory equipment and supplies for research use and drug development, namely primer plates, containers of metal and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids; laboratory instrument for the detection of pathogens in molecules, molecular proteins and peptides for research use other than medical use; scientific apparatus and instruments used in the measuring of molecules, molecular proteins and peptides for research use other than medical use; diagnostic apparatus and instruments used in scientific analysis, namely, diagnostic apparatus and instruments for the measurement of molecules and instruments for measuring proteins for non-medical purposes; downloadable software for measuring of molecules in the nature of molecule sequencing and data analysis for research use; kits for research use and drug development namely primer plates, containers and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. (1) Laboratory research regarding detection of molecules, molecular proteins and peptides; drug development; development of diagnostic preparations, namely, protein biomarkers for identifying diseases; diagnostic development, namely, custom design and development of chemical reagents and biochemical assays, biostatistics services, namely data analysis relating to protein biomarker analysis for scientific research purposes; laboratory research services regarding detection of molecules, proteins and peptides; laboratory biomarker analysis services and related data analysis.

Goods & Services

(1) Chemicals used in scientific analysis, research and for industrial use, namely Chemicals used in scientific analysis and detection of biomolecules, for determining the presence and quantity of a biomolecule in a sample including the steps of sample preparation and detection; biochemicals in the form of antibodies for research use; chemicals for detection of molecules, namely molecular proteins and peptides; chemical kits for analysing biological sample materials comprising sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers in the nature of diagnostic preparations and chemical preparations serving as control chemicals reagents for research use and use in drug development. (2) Chemicals for diagnostic use for medical or veterinary purposes, namely clinical diagnostic reagents; chemical preparations for biological sample analysis for medical diagnostic purposes. (3) Apparatus and instruments for scientific use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments other than for medical use; apparatus and instruments for diagnostic use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments for research use other than for medical use; laboratory instrument for the detection of pathogens in molecules, molecular proteins and peptides for research use other than medical use; scientific apparatus and instruments used in the measuring of molecules, molecular proteins and peptides for research use other than medical use; diagnostic apparatus and instruments used in scientific analysis, namely, diagnostic apparatus and instruments for the measurement of molecules and instruments for measuring proteins for non-medical purposes; downloadable software for measuring of molecules in the nature of molecule sequencing and data analysis for research use; supplies for research use and drug development namely primer plates, containers of metal and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. (1) Laboratory research services regarding detection of molecules, proteins and peptides; drug development; development of diagnostic preparations, namely, protein biomarkers for identifying diseases; diagnostic development, namely, custom design and development of chemical reagents and biochemical assays; data sciences, namely biostatistics services, namely data analysis relating to protein biomarker analysis for scientific research purposes; laboratory biomarker analysis services and related data analysis.

Goods & Services

Chemicals used in scientific analysis and research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical kits for analysing biological sample materials for scientific purposes comprising sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers in the nature of diagnostic preparations and chemical preparations serving as positive control chemicals reagents for research use and use in drug development Chemical preparations for biological sample analysis for medical diagnostic purposes Apparatus and instruments for scientific use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments; apparatus and instruments for diagnostic use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments for research use other than for medical use; scientific apparatus and instruments used in the measuring of molecules, molecular proteins and peptides other than for medical use; diagnostic apparatus and instruments used in scientific analysis, namely, diagnostic apparatus and instruments for the measurement of molecules and instruments for measuring proteins for non-medical purposes; downloadable software for measuring of molecules in the nature of molecule sequencing and data analysis; laboratory equipment and supplies for scientific research use and drug development, namely, primer PCR plates, containers and ampoules filled with incubation solutions, probe liquids being biochemical reagents, dilution and detection solutions as well as control liquids for use in testing and analyzing tissue and biological sample for non-medical purposes Laboratory research regarding detection of molecules, molecular proteins and peptides; pharmaceutical drug development services; diagnostic development, namely, custom design and development of chemical reagents and biochemical assays; data science and biostatistics services, namely, data analysis relating to protein biomarker analysis for scientific research purposes; laboratory biomarker analysis services and related data analysis

Goods & Services

Chemicals used in scientific analysis, research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical kits for analysing biological sample materials comprising sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers and control chemicals for research use and use in drug development. Chemicals for diagnostic use for medical or veterinary purposes. Apparatus and instruments for scientific and diagnostic use (other than medical use); apparatus and instruments used in the detection of molecules, molecular proteins and peptides (other than medical use); diagnostic apparatus, instruments and software used in scientific analysis and detection of molecules for non-medical purposes; laboratory equipment and supplies for research use and drug development namely primer plates, containers and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. Laboratory research regarding detection of molecules, molecular proteins and peptides; drug development; diagnostic development; data science (biostatistics) services; laboratory biomarker analysis services and related data analysis.

Goods & Services

Chemicals used in scientific analysis, research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical kits including sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers and control chemicals for research use and use in drug development. Chemicals for diagnostic use for medical or veterinary purposes. Apparatus and instruments for scientific and diagnostic use; apparatus and instruments used in the detection of molecules, molecular proteins and peptides; diagnostic apparatus, instruments and software used in scientific analysis and detection of molecules for non-medical purposes; kits for research use and drug development namely primer plates, containers and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. Research regarding detection of molecules, molecular proteins and peptides; drug development; diagnostic development; data science (biostatistics) services; laboratory biomarker analysis services and related data analysis.

Goods & Services

Chemicals used in scientific analysis, research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical kits including sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers and control chemicals for research use and use in drug development. Chemicals for diagnostic use for medical or veterinary purposes. Apparatus and instruments for scientific and diagnostic use; apparatus and instruments used in the detection of molecules, molecular proteins and peptides; diagnostic apparatus, instruments and software used in scientific analysis and detection of molecules for non-medical purposes; kits for research use and drug development namely primer plates, containers and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. Research regarding detection of molecules, molecular proteins and peptides; drug development; diagnostic development; data science (biostatistics) services; laboratory biomarker analysis services and related data analysis.

Abstract

The present invention relates to the identification of novel panels and combinations of biomarkers for ovarian cancer. An in vitro method is provided for detecting, predicting or monitoring ovarian cancer in a subject, wherein said method comprises determining in a sample from said subject the levels of the biomarkers in a panel comprising: TACSTD2, PROK1, MSMB, MUC-16, WFDC2, FR-alpha, and KRT19. More broadly, biomarkers may be selected and used from this list in variations combinations with each other and with other biomarkers. Also provided are sets of reagents for use in such methods.

IPC Classes  ?

• G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer

Goods & Services

Chemicals used in scientific analysis and research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical kits for analysing biological sample materials for scientific purposes comprising sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers in the nature of diagnostic preparations and chemical preparations serving as positive control chemicals reagents for research use and use in drug development chemical preparations for biological sample analysis for medical diagnostic purposes Apparatus and instruments for scientific use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments; apparatus and instruments for diagnostic use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments for research use other than for medical use; scientific apparatus and instruments used in the measuring of molecules, molecular proteins and peptides other than for medical use; diagnostic apparatus and instruments used in scientific analysis, namely, diagnostic apparatus and instruments for the measurement of molecules for non-medical purposes; downloadable software for measuring of molecules in the nature of molecule sequencing and data analysis; laboratory equipment and supplies for scientific research use and drug development, namely, primer PCR plates, containers and ampoules filled with incubation solutions, probe liquids being biochemical reagents, dilution and detection solutions as well as control liquids for use in testing and analyzing [ fluids ] * tissue and biological sample * for non-medical purposes Laboratory research regarding detection of molecules, molecular proteins and peptides; pharmaceutical drug development services; diagnostic development, namely, custom design and development of chemical reagents and biochemical assays; data science and biostatistics services, namely, data analysis relating to protein biomarker analysis for scientific research purposes; laboratory biomarker analysis services and related data analysis

Goods & Services

(1) Chemicals used in scientific analysis, research and for industrial use, namely chemicals used in scientific analysis and detection of biomolecules, for determining the presence and quantity of a biomolecule in a sample including the steps of sample preparation and detection; biochemicals in the form of antibodies for research use; chemicals for detection of molecules, namely molecular proteins and peptides; chemical kits for analysing biological sample materials comprising sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers in the nature of diagnostic preparations and chemical preparations serving as control chemicals reagents for research use and use in drug development. (2) Chemicals for diagnostic use for medical or veterinary purposes, namely clinical diagnostic reagents; chemical preparations for biological sample analysis for medical diagnostic purposes. (3) Apparatus and instruments for scientific use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments other than for medical use; apparatus and instruments for diagnostic use, namely, real-time PCR instruments for measuring of proteins and other molecules and sample preparation instruments for research use other than for medical use; laboratory instrument for the detection of pathogens in molecules, molecular proteins and peptides for research use other than medical use; scientific apparatus and instruments used in the measuring of molecules, molecular proteins and peptides for research use other than medical use; diagnostic apparatus and instruments used in scientific analysis, namely, diagnostic apparatus and instruments for the measurement of molecules and instruments for measuring proteins for non-medical purposes; downloadable software for measuring of molecules in the nature of molecule sequencing and data analysis for research use; laboratory equipment and supplies for research use and drug development namely primer plates, containers of metal and ampoules with incubation solutions, probe liquids, dilution and detection solutions as well as control liquids. (1) Laboratory research regarding detection of molecules, molecular proteins and peptides; drug development; development of diagnostic preparations, namely, protein biomarkers for identifying diseases; diagnostic development, namely, custom design and development of chemical reagents and biochemical assays; data sciences, namely biostatistics services, namely data analysis relating to protein biomarker analysis for scientific research purposes; laboratory biomarker analysis services and related data analysis.

Goods & Services

Chemicals used in scientific analysis, research and for industrial use; chemicals for detection of molecules including molecular proteins and peptides; chemical kits including sample dilution, antibodies, enzymes, detection solutions, polymerase; incubation stabilizers and control chemicals for research use and use in drug development. Chemicals for diagnostic use for medical or veterinary purposes. Apparatus and instruments for scientific and diagnostic use; apparatus and instruments used in the detection of molecules, molecular proteins and peptides; diagnostic apparatus, instruments and software used in scientific analysis and detection of molecules for non-medical purposes; kits for research use and drug development namely primer plates, containers and ampoules with incubation solutions, probe liquids, dilution and detection solutionms as well as control liquids. Research regarding detection of molecules, molecular proteins and peptides; drug development; diagnostic development; data science (biostatistics) services; laboratory biomarker analysis services and related data analysis.

Abstract

The present invention provides a plurality of pairs of proximity probes, each pair being capable of binding to a different target analyte, wherein the first and second proximity probes of each pair of probes comprise universal oligonucleotides conjugated to their analyte binding moieties, and hybridised to the universal oligonucleotides are different tag oligonucleotides comprising universal complement domains common to all tag oligonucleotides and unique domains unique to each tag oligonucleotide, as well as methods for their production.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6804 - Nucleic acid analysis using immunogens
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3′ exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3′ exonuclease activity; (d) extending the 3′ end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
• G01N 33/558 - ImmunoassayBiospecific binding assayMaterials therefor using diffusion or migration of antigen or antibody
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/6804 - Nucleic acid analysis using immunogens
• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The present invention provides a plurality of pairs of proximity probes, each pair being capable of binding to a different target analyte, wherein the first and second proximity probes of each pair of probes comprise universal oligonucleotides conjugated to their analyte binding moieties, and hybridised to the universal oligonucleotides are different tag oligonucleotides comprising universal complement domains common to all tag oligonucleotides and unique domains unique to each tag oligonucleotide, as well as methods for their production.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

Pyrococcus woesei (Pwo) DNA polymerase or a derivative or mutant thereof, preferably wherein said derivative is a sequence-modified derivative; and (d) amplifying and detecting the extension product.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3′ exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3′ exonuclease activity; (d) extending the 3′ end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
• G01N 33/558 - ImmunoassayBiospecific binding assayMaterials therefor using diffusion or migration of antigen or antibody

Abstract

The present invention relates to the use of a conjugate of a non-analyte-specific binding protein coupled to a nucleic acid as a blocking reagent in a probe-based detection assay, which uses a probe comprising a proteinaceous analyte-binding partner coupled to a nucleic acid domain to detect an analyte in a sample.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Goods & Services

chemicals used in the scientific analysis and detection of biomolecules. Chemicals for diagnostic use for pharmaceutical, medical or veterinary purposes. diagnostic apparatus and instruments and software for non-medical purposes.

Abstract

A method for detecting an analyte in a sample, comprising (a) contacting the sample with at least one set of at least first, second and third proximity probes, which probes each include an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte, the nucleic acid domain of the third proximity probe being a splint which is capable of hybridizing at least to the nucleic acid domains of the first and second proximity probes, wherein when all of the at least three proximity probes bind to the analyte, the nucleic acid domains of the first and second proximity probes are conjugatable by means of an interaction mediated by the hybridized splint of the third proximity probe; (b) conjugating the nucleic acids, of the first and second proximity probes; and (c) detecting the conjugation. Also provided is a kit for use in such a method.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

Methods of detecting affinity interactions between at least two molecules of interest are provided. The method comprises: a. forming a plurality of interactors by coupling each molecule of interest with at least one nucleic acid moiety comprising an identification sequence element and at an association element; b. promoting an association between at least two nucleic acid moieties from different interactors to form a plurality of unique associated oligonucleotides, wherein each nucleic acid moiety may form more than one unique associated oligonucleotide, and wherein each unique associated oligonucleotide comprises at least two identification sequence elements derived from the at least two nucleic acid moieties; c. selecting the plurality of unique associated oligonucleotides; and d. subjecting the selected associated oligonucleotides to an analysis that permits detection of the at least two identification sequence elements. Similar methods directed to detecting functional interactions, libraries of interactors employable in the present methods, and kits comprising those libraries are also provided.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• G01N 33/566 - ImmunoassayBiospecific binding assayMaterials therefor using specific carrier or receptor proteins as ligand binding reagent

Goods & Services

Chemicals used in scientific analysis and detection of biomolecules, for determining the presence and quantity of a biomolecule in a sample including the steps of sample preparation and detection [Chemicals for medical diagnostic use, used for determining the presence and quantity of a biomolecule for a diagnostic purpose in a sample including the steps of sample preparation and detection] Diagnostic apparatus and instruments for liquid handling and for detection of presence of microorganisms and biomolecules, said apparatus and instruments for non-medical purposes; software for non-medical use, namely, computer software for controlling liquid handling, optical and electrical detection of microorganisms and biomolecules, data analysis and user interface functions, all the aforementioned goods for use within the non-medical, life science research area

Goods & Services

Chemicals used in the scientific analysis and detection of biomolecules. Chemicals for diagnostic use. Diagnostic apparatus and instruments and software for non-medical purposes.