The present invention provides a method for determining the onset possibility or disease activity of IgA nephropathy by means of blood-mediated liquid biopsy, in addition to highly invasive renal biopsy and conventionally used proteinuria or hematuria. The present invention provides a method for determining the onset possibility or disease activity of IgA nephropathy in a test animal, the method comprising (a) a step for measuring an IgA nephropathy marker protein contained in an IgA immune complex in a blood sample collected from the test animal, and (b) a step for determining the onset possibility or disease activity of IgA nephropathy in the test animal on the basis of the amount of the IgA nephropathy marker protein measured in step (a), wherein the IgA nephropathy marker protein is a protein which is contained in the IgA immune complex, and with which the amount of the IgA nephropathy marker protein contained in the IgA immune complex in an IgA nephropathy patient group is greater than the amount contained in the IgA immune complex of a healthy subject group.
Provided are a uricase activator and a uricase activation method which are capable of highly activating uricase. Also provided are a uric acid measurement reagent and a uric acid measurement method which have a wide measurable concentration range. Further provided are a uricase activator comprising hydroxyisourate hydrolase, and a uric acid measurement reagent for use in measuring a uric acid concentration in a sample collected from a living body, comprising uricase and hydroxyisourate.
C12N 9/78 - Hydrolases (3.) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
Provided is a production method for obtaining pyridoxamine phosphate having high coenzyme activity with AST or ALT. For the production of pyridoxamine phosphate, pyridoxal kinase and pyridoxamine are brought into contact in the presence of adenosine triphosphate at a pyridoxamine concentration of 2 mM or more, and reacted until the percentage of pyridoxamine remaining is less than 5%.
C12Q 1/52 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase involving transaminase
4.
COMPOUND COMPRISING BETA-NICOTINAMIDE MONONUCLEOTIDE OR PHARMACOLOGICALLY ACCEPTABLE SALT THEREOF, AND METHOD FOR EVALUATING QUALITY AND METHOD FOR ASSESSING ENZYMATIC REACTIVITY OF SAID COMPOUND
A compound includes β-nicotinamide mononucleotide or a pharmacologically acceptable salt thereof. A purity of the compound as measured through HPLC is 95% or higher. A reactivity of the compound with lactate dehydrogenase is 30 units or higher.
The present invention provides a cereal flour composition which contains a cereal flour and an enzyme belonging to the tannase family, and in which the tannase activity per 1 g of the cereal flour is 0.1 kU/g to 60 kU/g. The composition preferably includes, in terms of mass, 10 ppm to 10,000 ppm of the enzyme relative to the total amount of the cereal flour. The cereal flour preferably includes at least one selected from among whole-wheat flour, bran, and other cereal flours having an ash content of 0.7 mass% or higher. The ash content of the cereal flour is preferably 0.5 mass% or higher.
The present invention provides a cereal flour composition which contains a cereal flour and an enzyme belonging to the tannase family, and in which the tannase activity per 1 g of the cereal flour is 0.1 kU/g to 60 kU/g. The composition preferably includes, in terms of mass, 10 ppm to 10,000 ppm of the enzyme relative to the total amount of the cereal flour. The cereal flour preferably includes at least one selected from among whole-wheat flour, bran, and other cereal flours having an ash content of 0.7 mass% or higher. The ash content of the cereal flour is preferably 0.5 mass% or higher.
Provided are a method and composition for stabilising nicotinamide adenine dinucleotide (NAD) in a biosample. A sample derived from a subject is brought into contact with nicotinamide and/or nicotinamide derivative to stabilise NAD in the sample.
C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
A medium for stem cells according to the present invention contains at least one of carboxymethyl cellulose and polyvinylpyrrolidone as a water-soluble polymer. The content of carboxymethyl cellulose in the medium is preferably such that the final concentration thereof is 0.001 μg/mL to 1 mg/mL. The content of polyvinylpyrrolidone in the medium is preferably such that the final concentration thereof is 0.05 μg/mL to 2 mg/mL.
The present invention provides a chromosome-stabilizing agent for stem cells containing a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, or a solvate thereof, as an active ingredient. The present invention also provides a culture method for stem cells, including culturing stem cells in a culture medium containing a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, or a solvate thereof.
An object of the present invention is to provide a material capable of further accelerating growth of pluripotent stem cells, such as pluripotent stem cells, without impairing pluripotency thereof. In other words, the invention is an agent for accelerating growth of pluripotent stem cells, containing a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof as an active ingredient; and is a method for culturing pluripotent stem cells, including culturing pluripotent stem cells in a culture medium that contains a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof.
Provided are a method for keeping qualities of cooked rice and a method for producing cooked rice, including a step of bringing a polyvalent cation-containing liquid into contact with rice at any timing between before and after rice boiling or rice steaming, and a step of bringing an alginate-containing liquid into contact with the rice that contains a polyvalent cation and has been boiled or steamed.
Provided are a uricase activator capable of high activation of uricase and a uricase activation method. Provided are a uric acid measurement reagent having a wide measurable concentration range and a uric acid measurement method. Provided are: a uricase activator that includes hydroxyisourate hydrolase; and a uric acid measurement reagent to be used to measure the uric acid concentration in a sample collected from a living organism, the uric acid measurement reagent including uricase and hydroxyisouric acid.
C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
Provided are a uricase activator capable of high activation of uricase and a uricase activation method. Provided are a uric acid measurement reagent having a wide measurable concentration range and a uric acid measurement method. Provided are: a uricase activator that includes hydroxyisourate hydrolase; and a uric acid measurement reagent to be used to measure the uric acid concentration in a sample collected from a living organism, the uric acid measurement reagent including uricase and hydroxyisouric acid.
C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
The stem cell medium according to the present invention contains, as a water soluble polymer compound, carboxymethyl cellulose and/or polyvinyl pyrrolidone. The added amount of carboxymethyl cellulose in the medium is preferably 0.001 µg/mL to 1 mg/mL in terms of the final concentration. Further, the added amount of polyvinyl pyrrolidone in the medium is preferably 0.05 µg/mL to 2 mg/mL in terms of the final concentration.
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
The present invention provides: a stabilizer for stem cell chromosomes, said stabilizer being characterized by comprising, as an active ingredient, β-nicotinamide mononucleotide, a pharmacologically acceptable salt thereof or a solvate of the same; and a method for culturing stem cells, said method being characterized by comprising culturing pluripotent stem cells in a culture medium containing β-nicotinamide mononucleotide, a pharmacologically acceptable salt thereof or a solvate of the same.
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
17.
COMPOUND COMPRISING β-NICOTINAMIDE MONONUCLEOTIDE OR PHARMACOLOGICALLY ACCEPTABLE SALT THEREOF, AND METHOD FOR EVALUATING QUALITY AND METHOD FOR ASSESSING ENZYMATIC REACTIVITY OF SAID COMPOUND
A compound comprising β-nicotinamide mononucleotide or a pharmacologically acceptable salt thereof, wherein the purity measured by HPLC is 95% or higher, and the reactivity to lactate dehydrogenase is 30 U or higher.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
18.
METHOD FOR KEEPING QUALITIES OF COOKED RICE AND METHOD FOR PRODUCING COOKED RICE
A method for keeping the qualities of cooked rice and a method for producing cooked rice, each method comprising a step for contacting rice with a liquid containing a polyvalent cation at any time from before cooking or steaming the rice to thereafter and a step for contacting the polyvalent cation-containing rice, which has been cooked or steamed, with a liquid containing an alginic acid salt.
A method for keeping the qualities of cooked rice and a method for producing cooked rice, each method comprising a step for contacting rice with a liquid containing a polyvalent cation at any time from before cooking or steaming the rice to thereafter and a step for contacting the polyvalent cation-containing rice, which has been cooked or steamed, with a liquid containing an alginic acid salt.
A23L 29/256 - Foods or foodstuffs containing additivesPreparation or treatment thereof containing gelling or thickening agents of vegetable origin from seaweeds, e.g. alginates, agar or carrageenan
The object of the present invention is to provide a material for efficiently obtaining differentiated cells from pluripotent stem cells. That is, the present invention relates to a pluripotent stem cell differentiation-promoting agent containing, as an active ingredient, a β-nicotinamide mononucleotide or a pharmacologically acceptable salt thereof, and a solvate thereof, and a method for differentiating pluripotent stem cells, including culturing pluripotent stern cells in a culture medium containing a β-nicotinamide mononucleotide or a pharmacologically acceptable salt thereof, and a solvate thereof.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Chemical additives for use in the manufacture of food, namely, quality improver for rice; Chemical additives for use in the manufacture of food, namely, quality improving agent; Food preserving chemicals, namely, shelf life improver
An object of the present invention is to provide a material which can be safely ingested and which inhibits skin pigmentation. Therefore, the invention is a skin pigmentation inhibitor, containing a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof; and a solvate thereof as an active ingredient; a health supplement which contains the skin pigmentation inhibitor and is ingested to inhibit skin pigmentation; and a method for inhibiting skin pigmentation, including ingesting the skin pigmentation inhibitor.
A61K 31/706 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
An object of the present invention is to provide a material capable of further accelerating growth of pluripotent stem cells, such as pluripotent stem cells, without impairing pluripotency thereof. In other words, the invention is an agent for accelerating growth of pluripotent stem cells, containing a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof as an active ingredient; and is a method for culturing pluripotent stem cells, including culturing pluripotent stem cells in a culture medium that contains a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof.
The present invention addresses the problem of providing a material for efficiently obtaining differentiated cells from pluripotent stem cells. Specifically, the present invention provides: a differentiation promoter for pluripotent stem cells that is characterized by having, as an active ingredient, β-nicotinamide mononucleotide, a pharmacologically acceptable salt thereof, or a solvate of either; and a method for causing pluripotent stem cells to differentiate that is characterized in that pluripotent stem cells are cultivated in a culture medium containing β-nicotinamide mononucleotide, a pharmacologically acceptable salt thereof, or a solvate of either.
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
A61K 31/706 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
Provided is an osteogenesis promoter for promoting the differentiation of osteoblast precursor cells into osteoblast cells, and the calcification of the osteoblast cells. The osteogenesis promoter includes, as an active ingredient, a substance which bonds to a TNF-related apoptosis-inducing ligand (TRAIL) receptor, said substance being a TRAIL or anti-TRAIL receptor antibody.
A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
A61P 3/14 - Drugs for disorders of the metabolism for electrolyte homeostasis for calcium homeostasis
A61P 19/02 - Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
A61P 19/08 - Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
A61P 19/10 - Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
A61P 29/00 - Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agentsNon-steroidal antiinflammatory drugs [NSAID]
A61P 43/00 - Drugs for specific purposes, not provided for in groups
C07K 14/51 - Bone morphogenic factorOsteogeninOsteogenic factorBone-inducing factor
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
The present invention addresses the problem of providing a material that can be safely ingested and suppresses skin pigmentation. In other words, the present invention provides: a skin pigmentation inhibitor characterized in that a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, or a solvate of these substances serves as an active ingredient thereof; a health supplement that contains said skin pigmentation inhibitor and is ingested in order to suppress skin pigmentation; and a method for suppressing skin pigmentation, said method including ingesting said skin pigmentation inhibitor.
A61K 31/706 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
The present invention addresses the problem of providing a material for further accelerating the growth of stem cells with differentiation potential, such as pluripotent stem cells, without impairing the differentiation potential thereof. That is, the present invention pertains to: an agent for accelerating the growth of stem cells with differentiation potential, characterized by containing, as active ingredients, β-nicotinamide mononucleotide (β-NMN) or a pharmaceutically acceptable salt thereof, and a solvate thereof; and a differentiation potential-having stem cells culturing method, characterized by culturing stem cells with differentiation potential in a culture medium containing β-nicotinamide mononucleotide (β-NMN) or a pharmaceutically acceptable salt thereof, and a solvate thereof.
The present invention is a method for producing citrus fruit flesh comprising: a step for (a) heat treatment and/or (b) organic acid treatment of the fruit of a citrus fruit that has at least an albedo and segment membranes; a step for separating the fruit into multiple or single sections; and a step for bringing the fruit into contact with at least one kind of enzyme selected from a group consisting of cellulase enzymes, pectinase enzymes and hemicellulase enzymes.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Chemicals; chemicals for use in food industry; conditioning
agents for food; chemical agents for improving the quality
of bread and cake; conditioning agents for confectionery and
bread; chemical agents for improving shelf life of
foodstuffs; chemical agents for improving quality of
foodstuffs; bacteriostats for the food industry; lye water,
lye powder(Kansui, food additive for Chinese noodles);
chemical agents for softening pounded rice cakes; enzymes;
lactic acid bacteria for use in food manufacture. Yeast; fresh yeast; dry yeast; flour paste (confectionery
and bakery creams made of wheat flour and starch as main
ingredients); flour paste in powder form; custard powder;
fermented flavor liquid; pre-ferment; sourdough powder;
baking powder; chemical leaveners; mayonnaise; salad
dressings; ketchup [sauce]; pizza sauce; yeast extract; malt
extract for food; brown rice (prepared); sprouted rice
(prepared); roasted rye.
The purpose of the present invention is to improve the storage stability, heat resistance and long-term stability of a flour paste and to reduce the cost required for the transportation, storage and production of a flour paste. The present invention provides a powder composition for preparing a flour paste, said powder composition comprising predetermined amounts of a modified starch, an unmodified starch, a milk whey protein and a thickening agent.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
31 - Agricultural products; live animals
Goods & Services
Chemicals for use in food and beverages industry;
conditioning agents for food; chemical agents for
conditioning quality of foodstuffs; chemical agents for
improving shelf life of foodstuffs; chemical agents for
improving quality of foodstuffs; bacteriostats for use in
industry; lye water (Kansui, food additive for Chinese
noodles); chemical agents for softening foodstuffs;
biochemistry reagent kit including chemical substances and
biochemical other than for medical and veterinary use;
enzymes; medium for microorganisms or medium for cell
cultures other than for medical and veterinary use; stem
cells other than for medical or veterinary purpose; protein;
other chemicals. Confectionery and bakery creams made of wheat flour and
starch as main ingredients; flour paste in powder form
(confectionery and bakery creams made of wheat flour and
starch as main ingredients); flour paste (confectionery and
bakery creams made of wheat flour and starch as main
ingredients); confectionery; bread and buns; sandwiches;
Chinese buns; hamburger sandwiches; pizzas; hot dogs
[sandwiches]; meat pies; pizza sauce; powdered mayonnaise
mixes; other seasonings [other than spices]; brown rice
(prepared); sprouted rice (prepared); processed grains;
Chinese stuffed dumplings; Chinese steamed dumplings; sushi;
fried balls of batter mix with small pieces of octopus
[Takoyaki]; boxed lunches consisting of rice, with added
meat, fish or vegetables; ravioli; yeast extract;
pre-ferment; yeast powder; koji [fermented malted rice];
yeast; baking powder; malt extract for food; custard powder;
instant confectionery mixes; fermented flavor liquid; food
flavorings other than food flavorings prepared from
essential oils; roasted rye; rice; husked oats; husked
barley; flour mixes; flour. Foodstuffs for animals; live animals; laboratory animals.
32.
BAKING POWDER, METHOD FOR MANUFACTURING SAME, AND FOOD USING BAKING POWDER
The purpose of the present invention is to provide a baking powder having excellent delayed effectiveness. Provided is a baking powder comprising sodium bicarbonate and acidic agents, said acidic agents having a median diameter of 100 μm or more and being coated with a fat having a melting point of 55-75oC, wherein: the acidic agents consist of one or more kinds of acidic agents having a median diameter of 100 μm or more and one or more kinds of acidic agents having a median diameter of less than 100 μm; and one or more kinds of acidic agents having a median diameter of 100 μm or more are surface-coated with the fat containing one or more kinds of acidic agents having a median diameter of less than 100 μm.
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (Japan)
NISSHIN SEIFUN GROUP INC. (Japan)
ORIENTAL YEAST CO.,LTD. (Japan)
Inventor
Oishi, Katsutaka
Itoh, Nanako
Yamamoto, Saori
Fukudome, Shin-Ichi
Kikuchi, Yosuke
Okita, Kimiko
Abstract
An agent for improving glucose tolerance disorder that comprises, as an active ingredient, partition chromatography peak components of an alcoholic extract of gramineous plant seeds, said peak components preferably comprising an alkyl resorcinol (AR) mixture containing multiple kinds of alkyl resorcinols. This mixture preferably contains an AR of general formula (I) wherein R1 is a saturated or unsaturated alkyl group having 15 carbon atoms, an AR of general formula (I) wherein R1 is a saturated or unsaturated alkyl group having 17 carbon atoms, an AR of general formula (I) wherein R1 is a saturated or unsaturated alkyl group having 19 carbon atoms, an AR of general formula (I) wherein R1 is a saturated or unsaturated alkyl group having 21 carbon atoms, an AR of general formula (I) wherein R1 is a saturated or unsaturated alkyl group having 23 carbon atoms, and an AR of general formula (I) wherein R1 is a saturated or unsaturated alkyl group having 25 carbon atoms, each in a specific amount. In general formula (I): R1 represents a saturated or unsaturated alkyl group having 15-25 carbon atoms; and R2 represents a hydrogen atom or a methyl group.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemicals; chemicals for use in food industry; chemical
agents for improving shelf life of foodstuffs; chemical
agents for improving quality of foodstuffs.
35.
Method of regulating circadian rhythm, and method of preparing circadian rhythm regulatory agent
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemicals for use in industry; chemicals for use in food industry; chemical agents for improving shelf life of foodstuffs; chemical agents for improving quality of foodstuffs
The purpose of the present invention is to provide: a formate dehydrogenase having a high specific activity; and a method for producing a formate dehydrogenase with high efficiency without using methanol. The present invention relates to: a novel formate dehydrogenase; a gene encoding the formate dehydrogenase; a method for producing the formate dehydrogenase; and a method for regenerating a coenzyme using the formate dehydrogenase.
Provided is a method for manufacturing a yeast extract containing copper includes an extraction step wherein yeast containing copper is suspended in a solution that contains an amino acid or a salt thereof and the solid components and liquid components of the suspension obtained are separated with the amino acid or salt thereof being water-soluble. Also provided are a yeast extract containing copper, a food product, and a green color preserving and restoring agent for vegetables.
Problem: To provide a preparation capable of easily and efficiently improving the quality of filling-containing breads, in particular, filling-containing breads in which the amount of filling is comparatively large and/or the water content in the filling is high, and a production method, and the like, for breads using the preparation. Solution: By adding and mixing an alginate ester, powdered egg yolk, and modified starch in bread dough for filling-containing breads, the migration of moisture from the filling to the bread part is suppressed and sogginess of the bread in such breads is suppressed even if there is a migration of moisture, peeling of the bread crust is suppressed, sticking of the bread is suppressed, and the bread is imparted with a flaky texture, as a result of which the bread quality can be improved.
Problem: To provide a bread quality improving agent suitable for frozen dough with which it is possible to prepare breads from frozen bread dough without a decrease in the quality (for example, the bread volume, bread height, specific volume, crust quality, etc.) of the breads, and a production method, and the like, for breads using the improving agent. Solution: A bread quality improving agent for frozen dough is used which includes transglutaminase, L-ascorbic acid, and a bread emulsifier. In addition, bread dough is prepared combined with the bread quality improving agent for frozen dough, the bread dough is frozen following any of the steps of primary fermentation, shaping, and final fermentation, and breads are produced therefrom according to conventional methods.
A method for producing a high-zinc-content yeast extract, including extracting the high-zinc-content yeast extract by suspending zinc-containing yeast into a solution containing a carboxylic acid, a carboxylic acid salt, or both thereof to thereby obtain a suspension liquid, and separating a solid component and a liquid component of the suspension liquid; the high-zinc-content yeast extract; food; and an agent for maintaining and restoring a green color of vegetables.
Provided are: a method that is for producing a high-iron-content yeast extract and that contains an extraction step for suspending iron-containing yeast in a solution containing a carboxylic acid and/or a carboxylate, and separating the solid component and liquid component of the obtained suspension; a high-iron-content yeast extract; and a food product.
[Problem] To provide: a novel quality-preserving agent which can preserve the quality of a peeled and/or cut vegetable or fruit or a seafood during storage in a simple manner and effectively and is highly safe; and others. [Solution] The quality-preserving agent contains two components, i.e., sodium acetate and α-lipoic acid, as active ingredients. The quality-preserving agent can prevent the discoloration, brownish discoloration or the like of a peeled and/or cut vegetable or fruit or a seafood during chilled storage and ambient-temperature storage of the vegetable or fruit or the seafood, can improve the shelf life of the vegetable or fruit or the seafood, and can preserve the quality of the vegetable or fruit or the seafood in a simple manner and effectively merely by bringing the vegetable or fruit or the seafood into contact with the quality-preserving agent.
A method for producing a manganese-rich yeast extract, which involves an extraction step of suspending manganese-containing yeast in a solution containing a carboxylic acid and/or a carboxylic acid salt and then separating the resultant suspension into a solid component and a liquid component; a manganese-rich yeast extract; a food; a composition for a culture medium for an edible lactic acid bacterium; a culture medium for an edible lactic acid bacterium; and a method for culturing an edible lactic acid bacterium.
[Problem] To provide a highly safe preparation, method and the like whereby the qualities of at least one product selected from among breads, confectioneries, doughnuts, pies and Chinese dumpling skins can be easily and effectively improved. [Solution] A quality improving agent which comprises, as active ingredients, glycine and a pentose and/or a hexose. By kneading the quality improving agent into a dough, at least one quality improving effect can be exerted on at least one product selected from among breads, confectioneries, doughnuts, pies and Chinese dumpling skins, said quality improving effect being selected from, in particular: imparting a baked and/or fried brown color and promoting browning; shortening the baking and/or frying time; lowering the baking and/or frying temperature; reducing moisture loss in the baking and/or frying step and improving the texture; preventing skin-peeling and cracking in baked frozen breads or parbaked frozen products; and thinning the crust film of baked breads and thus improving the texture and increasing the crumb ratio.
A method for producing a yeast extract with a high copper content, said method being characterized by compirising an extraction step for suspending a copper-containing yeast in a solution containing a carboxylic acid and/or a carboxylic acid salt and then separating the solid component of the obtained suspension from the liquid component; a yeast extract with a high copper content; and an agent for maintaining and restoring the green color of foods and vegetables.
[Problem] To provide a novel quality-keeping agent with a high safety whereby the qualities of peeled and/or cut vegetables, fruits and so on can be easily and effectively maintained during storage. [Solution] During cold or room-temperature storage of peeled and/or cut vegetables or fruits, color change, browning and so on can be prevented and the qualities can be easily and effectively maintained simply by using, as the active components, three components (i.e., sodium citrate, alum and α-lipoic acid) or four components (i.e., sodium citrate, alum, α-lipoic acid and sodium tartrate) and contacting the peeled and/or cut vegetables or fruits therewith.
Provided is a method for producing a liquid fermented milk having favorable flavor and appearance, having no occurrence of solid content floating up, having no contained large particles observed with the naked eye after completion of stirring fermentation, and having a rich aroma and the like deriving from fermentation by yeast. The method for producing a liquid fermented milk contains: (A) a static fermentation step that, after adding a lactobacillus to the starting material for fermented milk, causes static fermentation to obtain a lactic acid fermentation product; (B) a stirring fermentation step that, after adding yeast to the lactic acid fermentation product obtained in step (A), causes stirring fermentation to obtain a liquid fermented milk; and (C) a homogenization step for subjecting the liquid fermented milk obtained in step (B) to homogenization processing.
A23C 9/127 - Fermented milk preparationsTreatment using microorganisms or enzymes using microorganisms of the genus lactobacteriaceae and other microorganisms or enzymes, e.g. kefir, koumiss
A23C 9/13 - Fermented milk preparationsTreatment using microorganisms or enzymes using additives
51.
METHOD FOR PRODUCING ZINC-RICH YEAST EXTRACT, ZINC-RICH YEAST EXTRACT, AND GREEN-RETAINING/-RESTORING AGENT FOR FOOD AND VEGETABLES
A method for producing a high-zinc-content yeast extract, including extracting the high-zinc-content yeast extract by suspending zinc-containing yeast into a solution containing a carboxylic acid, a carboxylic acid salt, or both thereof to thereby obtain a suspension liquid, and separating a solid component and a liquid component of the suspension liquid; the high-zinc-content yeast extract; food; and an agent for maintaining and restoring a green color of vegetables.
A method for producing a zinc-rich yeast extract, the method comprising an extraction step for suspending a yeast containing zinc in a solution including carboxylic acid and/or carboxylic acid salt, and separating the solid component and liquid component of the resulting suspension; a zinc-rich yeast extract; and a green-retaining/-restoring agent for food and vegetables.
The purpose of the present invention is to provide a method for culturing a mammalian cell in a system containing laminin 511. The method according to the present invention is characterized by culturing the cell under such conditions that a blood protein other than an extracellular matrix protein, which is serum, serum albumin, prealbumin, an immunoglobulin, α-globulin, β-globulin, α1-antitrypsin (α1-AT), haptoglobin (Hp), α2-macroglobulin (α2-M), α-fetoprotein (AFP), transferrin, a retinol-binding protein (RBP) or adiponectin, a polypeptide and/or a peptide selected from the group consisting of gelatin, a protein belonging to a tumor necrosis factor (TNF) family and peptone, and laminin 511 are solid-phased.
The objective of the present invention is to provide: a method for producing frozen whipping cream wherein the cream can be refrigerated, the creaminess of the cream after thawing is the same as refrigerated cream, and quality in terms of smoothness and melting in the mouth when made into whipped cream is the same as whipped cream made by whipping refrigerated cream; frozen whipping cream produced by the method; and whipped cream using the frozen whipping cream and a method for making the same. The present invention is: a method for producing frozen whipping cream which includes a cooling step for cooling fresh cream, the fresh cream being passed from 0°C to -5°C in 8 minutes or less in the cooling step; frozen whipping cream produced by the method; whipped cream using the frozen whipping cream; and a method for making the same.
A method for producing frozen fresh cream to be whipped, the method including: cooling fresh cream, wherein in the cooling, the fresh cream is cooled to a temperature of −5° C. or lower and is changed from 0° C. to −5° C. for 8 minutes or shorter.
The invention provides a potentiator of cancer immunity containing a compound which blocks the action of RANKL. The invention is a potentiator of cancer immunity containing, as an active ingredient, a RANKL antagonist such as an anti-RANKL neutralizing antibody.
The invention provides a potentiator of cancer immunity containing a compound which blocks the action of RANKL. The invention is a potentiator of cancer immunity containing, as an active ingredient, a RANKL antagonist such as an anti-RANKL neutralizing antibody.
Disclosed is a method which improves the quality of at least one item chosen from bread, noodles and sweets, and which imparts a softness and moistness and makes the item not prone to aging even after a certain period has elapsed after manufacture (aging is suppressed and prevented); also disclosed is an agent used in said method. Softness and moistness can be imparted to at least one item chosen from bread, noodles and sweets, and aging can be suppressed and prevented during storage after manufacture by adding to wheat flour and/or rice flour, which is the main ingredient, four components: α-amylase, β-amylase and a partial α-starch, and a processed product of seaweed belonging to the genus Lessonia of the order Laminariales.
Disclosed is a method for culturing cells in a system containing laminin-5. This method is characterized by including a culture system for polypeptides selected from a group consisting of serum, serum albumin, prealbumin, immunoglobulin, alpha globulins, beta globulins, α1-antitrypsin (α1-AT), haptoglobin (Hp), α2-macroglobulin (α2M), α-fetoprotein (AFP), transferrin, retinol binding protein (RBP), and proteins in the blood other than extracellular matrix proteins as well as gelatin, proteins belonging to the tumor necrosis factor (TNF) family and peptones.
C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
C07K 14/79 - Transferrins, e.g. lactoferrins, ovotransferrins
60.
Fusion protein composed of matrix metalloproteinase-2 inhibitor peptide derived from amyloid-B precursor protein and tissue inhibitor of metalloproteinase-2
The present invention provides an agent capable of inhibiting MMP-2 specifically. Disclosed is a fusion molecule composed of a β-amyloid precursor protein molecule-derived domain having an activity of selectively inhibiting matrix metalloproteinase-2 and a tissue inhibitor of metalloproteinase capable of binding to latent matrix metalloproteinase. Also disclosed are a pharmaceutical composition, a cancer metastasis and/or angiogenesis inhibitor, a therapeutic and/or prophylactic for cardiovascular diseases, and a matrix metalloproteinase-2 inhibitor, each of which comprises the fusion molecule.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
61.
Inducer of chondrocyte proliferation and differentiation
This invention provides a method for administration of an effective amount of RANKL-binding molecules that act on prechondrocytes and/or mesenchymal stem cells, accelerate cartilage differentiation, proliferation, and maturation of such cells, enhance chondrocyte differentiation, and induce chondrocyte proliferation to induce chondrocyte proliferation and differentiation or increase cartilage matrix production and a pharmaceutical composition used for inducing chondrocyte proliferation and differentiation or increasing cartilage matrix production. The pharmaceutical composition used for treatment or prevention of a chondropathies comprises, as an active ingredient, a compound that acts on prechondrocytes and/or mesenchymal stem cells and induces at least one of the following: (a) acceleration of prechondrocyte and/or mesenchymal stem cell differentiation; (b) acceleration of prechondrocyte and/or mesenchymal stem cell proliferation; (c) acceleration of prechondrocyte and/or mesenchymal stem cell maturation; (d) enhancement of chondrocyte differentiation; (e) chondrocyte proliferation; and (f) increased production of the cartilage matrix.
Provided is a technique for observing in real time the state of a disease condition in a tissue of an animal or the state of a functionally adverse condition which is a prelude to the disease condition without injuring the animal. This can be achieved by the use of a gene construct having a reporter gene integrated under the control of a hypoxia responsible promoter an ODD domain (oxygen dependent degradation domain) integrated upstream to the reporter gene.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Chemical additives for use in the manufacture of food; KANSUI, namely, food additive for making Chinese noodle; biochemical reagents, namely, diagnostic reagents for clinical laboratory use; enzymes for food manufacturing; enzymes for the bakery industry as ingredients used in the manufacture of biochemistry reagents.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
29 - Meat, dairy products, prepared or preserved foods
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Quality improving agents for confectionery and bakery; dough conditioners; dough stabilizers; kansui (food additive for manufacture of Chinese noodle); ingredients for use in the manufacture of foods; preservatives for foodstuffs; bacteriostatic agents for foodstuffs; diagnostic reagents and preparations, except for medical or veterinary use; reagents for scientific purposes; immunochemistry reagents; biochemistry reagents; enzymes [other than for medical or veterinary use]; culture media for microbiology; flavour enhancers for foodstuffs; chemical products for the preparation of flavourings; softening preparations for pounded rice cakes (mochi) (chemical preparations). Dietary food supplements; powdered and granulated processed dietary food supplements made of yeast; biological and chemical preparations and reagents for medical or veterinary use; enzymes for medical or veterinary use. Buttercream; curry [prepared meals with or without rice]; prepared sweet or fruit fillings. Yeast; yeast extracts; confectionery made mainly of wheat flour and starches; confectionery and bakery creams (paste) made mainly from wheat flour and starches; custard powder; curry powder; curry paste; curry sauces; bakery products; mixes for making bakery products; pre-ferment; baking powder; mayonnaise; powdered mayonnaise; dressings for food; rice; brown rice (prepared); sprouted rice; roasted rye; flavourings and essences for food and beverages; fermented liquid flavouring; dough; dough mix; softening preparations for pounded rice cakes (mochi) (other than chemical preparations).
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemicals for industrial purposes; chemical additives for use in the manufacture of food; Kansui, namely, chemical additives for use in the manufacture of food; [ bacteriostatic agents for use in the manufacture of food; ] Biochemical reagents used for non-medical purposes; [ Enzymes for use in the food industry; ]enzymes as ingredients used in manufacture of biochemistry reagents; [ Bacteria for use in food manufacture ]
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Chemical additives for use in the manufacture of food; KANSUI, namely, food additive for making Chinese noodle; biochemical reagents, namely, diagnostic reagents for clinical laboratory use; enzymes for food manufacturing; enzymes for the bakery industry as ingredients used in the manufacture of biochemistry reagents.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
29 - Meat, dairy products, prepared or preserved foods
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Quality improving agents for confectionery and bakery; dough conditioners; dough stabilizers; kansui (food additive for manufacture of Chinese noodle); ingredients for use in the manufacture of foods; preservatives for foodstuffs; bacteriostatic agents for foodstuffs; diagnostic reagents and preparations, except for medical or veterinary use; reagents for scientific purposes; immunochemistry reagents; biochemistry reagents; enzymes [other than for medical or veterinary use]; culture media for microbiology; flavour enhancers for foodstuffs; chemical products for the preparation of flavourings; softening preparations for pounded rice cakes (mochi) (chemical preparations). Dietary food supplements; powdered and granulated processed dietary food supplements made of yeast; biological and chemical preparations and reagents for medical or veterinary use; enzymes for medical or veterinary use. Buttercream; curry [prepared meals with or without rice]; prepared sweet or fruit fillings. Yeast; yeast extracts; confectionery made mainly of wheat flour and starches; confectionery and bakery creams (paste) made mainly from wheat flour and starches; custard powder; curry powder; curry paste; curry sauces; bakery products; mixes for making bakery products; pre-ferment; baking powder; mayonnaise; powdered mayonnaise; dressings for food; rice; brown rice (prepared); sprouted rice; roasted rye; flavourings and essences for food and beverages; fermented liquid flavouring; dough; dough mix; softening preparations for pounded rice cakes (mochi) (other than chemical preparations).
69.
Method of separating and distinguishing walnut from pecan nut
Provided is a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut. The method involves a PCR method for specifically detecting nuts of the family Juglandaceae and identifying the matK sequence of pecan nut to thereby discriminate walnut and pecan nut from each other optionally using restriction enzymes.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
70.
Polypeptide for unstabilizing protein in cells under aerobic conditions and DNA encoding the same
To identify a domain in HIF-1α protein, which participates in stabilization of a fused protein, DNA encoding the following polypeptide (A) or (B) is provided:
(A) a polypeptide having the amino acid sequence of SEQ ID NO: 1
(B) a polypeptide having an amino acid sequence comprising at least 16 amino acid residues in the amino acid sequence of SEQ ID NO: 1, and imparting stability dependent on an oxygen concentration to other protein in a cell harboring a fused protein, when the polypeptide is fused with a nuclear localization signal and the other protein to form the fused protein.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
71.
FUSION PROTEIN COMPOSED OF MATRIX METALLOPROTEINASE-2 INHIBITOR PEPTIDE DERIVED FROM AMYLOID-Β PRECURSOR PROTEIN AND TISSUE INHIBITOR OF METALLOPROTEINASE-2
Disclosed is a substance capable of inhibiting MMP-2 specifically. Specifically disclosed is a fusion molecule composed of: a region which has an activity to selectively inhibit matrix metalloproteinase-2 and is contained in an amyloid-β precursor protein molecule; and a tissue inhibitor of metalloproteinase which can bind to a latent matrix metalloproteinase. Also disclosed are a pharmaceutical composition, a cancer metastasis and/or angiogenesis inhibitor, a therapeutic and/or prophylactic agent for cardiovascular diseases, and a matrix metalloproteinase-2 inhibitor, each of which comprises the fusion molecule.
A61P 9/00 - Drugs for disorders of the cardiovascular system
A61P 9/04 - Inotropic agents, i.e. stimulants of cardiac contractionDrugs for heart failure
A61P 9/10 - Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
A61P 35/04 - Antineoplastic agents specific for metastasis
A61P 43/00 - Drugs for specific purposes, not provided for in groups
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
The present invention provides a method for producing an osteopenia animal model by RANKL administration and an osteopenia animal model.
Also, a method for producing an osteopenia animal model, comprising administering soluble RANKL or a fused protein of soluble RANKL with an epitope tag to a non-human animal so as to promote vivo osteoclast differentiation and activation in the non-human animal, and an osteopenia animal model produced by the method are provided.
Disclosed is a method for inducing the proliferation/differentiation of a cartilage cell or increasing the production of a cartilage substrate by administering an effective amount of a RANKL-binding molecule which can act on a cartilage progenitor cell and/or a mesenchymal stem cell to promote the differentiation of the cartilage progenitor cell and/or the mesenchymal stem cell into a cartilage or the proliferation or maturation of the cartilage progenitor cell and/or the mesenchymal stem cell, enhance the differentiation of a cartilage cell and induce the proliferation of a cartilage cell. Also disclosed is a pharmaceutical composition for inducing the proliferation/differentiation of a cartilage cell or increasing the production of a cartilage substrate. Specifically disclosed is a pharmaceutical composition for treating or preventing a cartilage disease, which comprising a compound capable of acting on a cartilage progenitor cell and/or a mesenchymal stem cell to induce at least one phenomenon selected from the following items (a) to (f) as an active ingredient: (a) the promotion of the differentiation of the cartilage progenitor cell and/or the mesenchymal stem cell; (b) the promotion of the proliferation of the cartilage progenitor cell and/or the mesenchymal stem cell; (c) the promotion of the maturation of the cartilage progenitor cell and/or the mesenchymal stem cell; (d) the enhancement of the cartilage cell differentiation; (e) the proliferation of a cartilage cell; and (f) the increase in the production of a cartilage substrate.
Disclosed are: a standard molecule characterized in that the presence of a false-positive result caused by unintended contamination of a reagent, a sample or the like with the standard molecule can be confirmed simultaneously with the determination of the test results in real-time PCR test; a primer; a probe; and a test method using the standard molecule, the primer and the probe. In a real-time PCR test, the presence of a false-positive result caused by contamination with a standard molecule for a real-time PCR test can be determined simultaneously by carrying out the test using the standard molecule and a primer/probe, wherein the standard molecule contains both an artificial DNA sequence and a target region of DNA to be tested, wherein the artificial DNA sequence can be amplified under the same PCR conditions and in the same reaction vessel as those for the target region of the DNA to be tested in the real-time PCR test, and wherein the primer/probe is one used for amplifying/detecting only the DNA sequence.
To newly develop a system for imparting heat resistance to a food antioxidant (in particular, a food antioxidant containing β-amylase as the active ingredient). To solve the above-described problem, a material for imparting heat resistance to a food antioxidant containing β-amylase as the active ingredient that is characterized by being at least one member selected from among a yeast-treated material, a yam powder and a modified cellulose and capable of imparting heat resistance of 90°C or higher has been developed.
A21D 13/00 - Finished or partly finished bakery products
A21D 13/08 - Pastry, e.g. cake, biscuit, puff-pastry (icing or frosting or mixes therefor A23G 3/00)
A23G 3/34 - Sweetmeats, confectionery or marzipanProcesses for the preparation thereof
A23G 3/48 - Sweetmeats, confectionery or marzipanProcesses for the preparation thereof characterised by the composition containing plants or parts thereof, e.g. fruits, seeds, extracts
Disclosed is a method for producing a recombinant protein efficiently by expressing the recombinant protein in a eukaryotic cell host and releasing the recombinant protein to the outside of the cell. Specifically disclosed is a polynucleotide which can be used for producing a recombinant protein in a host cell. The polynucleotide comprises: a polynucleotide encoding an endoplasmic reticulum-insertion signal sequence and a glycosylated sequence comprising a sequence represented by the formula: Asn-X-(Thr/Ser) [wherein X represents an amino acid residue other than proline]; and a polynucleotide encoding a protein of interest that cannot be released efficiently to the outside of a host even when the protein is fused with an endoplasmic reticulum-insertion signal sequence. The polynucleotide enables the release of the protein of interest to the outside of the hose cell in a glycosylation-dependent manner.
Disclosed is an agent for improving at least one activity selected from the group consisting of the growth activity, adhesion activity and extension activity of mesenchymal stem cells, which comprises laminin-5 as an active ingredient. A method of culturing mesenchymal stem cells; a method of isolating mesenchymal stem cells; and a medium, vessel or sheet for use in culturing mesenchymal stem cells are also provided.
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 5/02 - Propagation of single cells or cells in suspensionMaintenance thereofCulture media therefor
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
The object aims to proliferate a pluripotent stem cell efficiently in a system which does not use any animal-derived material such as a feeder cell or a serum. Thus, disclosed is a method for proliferating a pluripotent stem cell, which comprises culturing the pluripotent stem cell in a culture medium containing no feeder cell or serum in a system containing laminin-5.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
The object aims to proliferate a pluripotent stem cell efficiently in a system which does not use any animal-derived material such as a feeder cell or a serum. Thus, disclosed is a method for proliferating a pluripotent stem cell, which comprises culturing the pluripotent stem cell in a culture medium containing no feeder cell or serum in a system containing laminin-5.
A novel method for detection of an inflammatory disease and a novel composition for prevention or treatment of an inflammatory disease are provided. The method for detection of an inflammatory disease comprises using RANKL and/or OPG as a marker in a biological sample. The composition for prevention or treatment of an inflammatory disease comprises RANKL and/or M-CSF as an active ingredient.
This object aims to provide a yeast which has a high safety, can exerts a high immunopotentiating effect even in the case of being taken as cells, is easily available and less expensive, can be directly employed in producing foods and can be cultured even at a low osmotic pressure, and a food containing this yeast. A yeast characterized by containing only a small amount of mannan in the cell wall, having an immunopotentiating effect and being capable of growing in YPD liquid medium having an osmotic pressure of 300 m Osm. The above-described yeast is preferably either of Saccharomyces cerevisiae FERM AP-21354 or Saccharomyces cerevisiae FERM AP-21355.
Disclosed is a bone formation enhancing agent comprising an RANKL-acting molecule which can enhance the differentiation, maturation or calcification of an osteoblast or a cell capable of being differentiated into an osteoblast. Specifically disclosed is a pharmaceutical composition for the treatment or prevention of a metabolic bone disease accompanied by the decrease in bone mass, which comprises, as an active ingredient, a compound which can act on an RANKL located on an osteoblast or a cell capable of being differentiated into an osteoblast to accelerate the differentiation, proliferation, maturation or calcification of the osteoblast or the cell capable of being differentiated into an osteoblast.
It is intended to provide a determination method for an allergic disease which is capable of performing a multidimensional and comprehensive analysis with a small amount of a specimen and particularly suppressing a non-specific reaction as much as possible thereby to enable an accurate measurement with high sensitivity even if a body fluid other than blood such as saliva, nasal discharge or lacrimal fluid is used as the specimen. After a chemically-modified diamond/DLC (Diamond-like Carbon) chip is activated by an activating reagent, a coupling reaction of an allergen or a peptide containing an allergen epitope is performed and a specimen (such as saliva, nasal discharge or lacrimal fluid) subjected to pressure filtration with a low protein adsorptive filter is subsequently brought into contact with an allergen determination chip subjected to a washing and blocking procedure against an unreacted active group, and an allergen recognition antibody in the specimen captured by the allergen determination chip is detected through an immunoassay using a labeled secondary antibody. At this time, as a washing liquid and/or a blocking liquid to be used in the washing and blocking procedure, a glycine-containing liquid is used.
G01N 21/78 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
G01N 33/531 - Production of immunochemical test materials
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
84.
METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT
[PROBLEMS] To newly develop a method whereby walnut can be conveniently, quickly and accurately separated and distinguished from pecan nut. [MEANS FOR SOLVING PROBLEMS] With the recent increase in the patients with walnut allergy and an increase in pecan nut imports in Japan, it has been urgently required to develop a method of distinguished walnut from pecan nut. Paying attention to chloroplast matK gene having a clarified gene sequence, a PCR method for specifically detecting a nut belonging to the family Juglandaceae is developed. To distinguish walnut from pecan nut, furthermore, pecan nut matK sequence is clarified and thus amethod of distinguished walnut from pecan nut with the use of a restriction enzyme is established.
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
It is intended to provide a novel method of detecting mammary cancer cells with the use of a change in the expression level of a specific gene in a human mammary cancer tissue (or cells) as an indication. This object is achieved by providing a detection method characterized by comprising: (1) measuring the expression level of a gene having a specific base sequence in a human mammary cancer tissue (or cells); (2) measuring the expression level of the above-described gene in a normal human mammary gland tissue (or cells); and (3) comparing the values measured in the above (1) and (2) and thus detecting mammary cancer cell on the basis of the difference therein.
It is intended to provide a reagent containing a fused protein of RANKL with an epitope tag which has an improved effect of differentiating and activating osteoclasts compared with the case of using RANKL alone and an improved storage stability. An agent of differentiating and activating osteoclasts in vitro or in vivo which contains a fused protein of soluble RANKL with an epitope tag peptide as the active ingredient.
C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12N 5/078 - Cells from blood or from the immune system
A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
The present invention provides a method for producing an osteopenia animal model by RANKL administration and an osteopenia animal model. Also, a method for producing an osteopenia animal model, comprising administering soluble RANKL or a fused protein of soluble RANKL with an epitope tag to a non-human animal so as to promote in vivo osteoclast differentiation and activation in the non-human animal, and an osteopenia animal model produced by the method are provided.
It is intended to provide a method of constructing a bone loss model animal by administering RANKL and a bone loss model animal. Namely, a method of constructing a bone loss model animal which comprises administering soluble RANKL or a fused protein of soluble RANKL with an epitope tag to a nonhuman animal and thus promoting the differentiation and activation of osteoclasts in the body of the nonhuman animal; and a bone loss model animal constructed by this method.
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
Disclosed are: a method for producing an osteopenia model animal by administering RANKL; and an osteopenia model animal. Specifically disclosed are: a method for producing an osteopenia model animal, which comprises administering soluble RANKL or a fusion protein of soluble RANKL and an epitope tag to a non-human animal to stimulate the differentiation and activation of an osteoclast in the non-human animal; and an osteopenia model animal produced by the method.
It is intended to provide a reagent containing a fused protein of RANKL with an epitope tag which has an improved effect of differentiating and activating osteoclasts compared with the case of using RANKL alone and an improved storage stability. An agent of differentiating and activating osteoclasts in vitro or in vivo which contains a fused protein of soluble RANKL with an epitope tag peptide as the active ingredient.
A novel method for detection of an inflammatory disease and a novel composition for prevention or treatment of an inflammatory disease are provided. The method for detection of an inflammatory disease comprises using RANKL and/or OPG as a marker in a biological sample. The composition for prevention or treatment of an inflammatory disease comprises RANKL and/or M-CSF as an active ingredient.
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
A61P 31/00 - Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
A61P 37/00 - Drugs for immunological or allergic disorders
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
Disclosed are a novel method for detecting an inflammatory disease and a novel composition for the prevention or treatment of an inflammatory disease. A method for detecting an inflammatory disease using RANKL and/or OPG in a biological sample as a marker; and a composition for the prevention or treatment of an inflammatory disease comprising RANKL and/or M-CSF as an active ingredient.
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
A61P 31/00 - Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
A61P 37/00 - Drugs for immunological or allergic disorders
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
National Institute of Radiological Sciences (Japan)
Inventor
Saito, Toshiyuki
Mikami, Yoji
Kinugasa, Masahiro
Mori, Kazuya
Sugimoto, Michiyo
Uchida, Koji
Abstract
It is intended to provide a novel method for determining the possibility of lymph node metastasis of breast cancer using a difference of expression level of a specific gene between a human metastatic breast cancer tissue (or cell) and a nonmetastatic breast cancer tissue (or cell) as an index. For this purpose, the method characterized by: (1) measuring the expression level of a gene having a specific base sequence in a human metastatic breast cancer tissue (or cell); (2) measuring the expression level of the gene in a human nonmetastatic breast cancer tissue (or cell); and (3) comparing the measured values of (1) and (2) and determining the possibility of lymph node metastasis of breast cancer based on the difference is provided.
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
KIHARA MEMORIAL YOKOHAMA FOUNDATION FOR THE ADVANCEMENT OF LIFE SCIENCES (Japan)
Inventor
Miyazaki, Kaoru
Hashimoto, Junko
Kariya, Yoshinobu
Abstract
Disclosed is a substance capable of enhancing at least one activity selected from the group consisting of growth activity, adhesion activity and extension activity of a mesenchymal stem cell. The substance comprises laminin-5 as an active ingredient. Also disclosed are a method for cultivation of a mesenchymal stem cell, a method for isolation of a mesenchymal stem cell, and a culture medium, vessel or sheet for use in the cultivation of a mesenchymal stem cell.
ABSTRACT Saccharomyces FD 612 is very useful as a baker's yeast which has been produced by many selections and matings started from three kinds of yeasts, since it has an excellent freeze-resistance and can produce bread with a good quality even after the freeze-storage of a dough.
