The present disclosure relates to a method of classifying a patient that suffers from or is at risk of developing urothelial or bladder cancer. The method involves the steps of taking a liquid sample from the patient, extracting RNA from the liquid sample, and determining in the RNA, the expression level of at least one gene encoding for a receptor selected from the group consisting of FGFR2, FGFR3 or ErbB2, and classifying the sample of the patient into one of at least two classifications.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample and for stabilizing the transcriptome of contained cells.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C07C 233/05 - Amides d'acides carboxyliques ayant des atomes de carbone de groupes carboxamide liés à des atomes d'hydrogène ou à des atomes de carbone acycliques ayant les atomes d'azote des groupes carboxamide liés à des atomes d'hydrogène ou à des atomes de carbone de radicaux hydrocarbonés non substitués avec des atomes de carbone de groupes carboxamide liés à des atomes de carbone d'un squelette carboné saturé acyclique ayant les atomes d'azote des groupes carboxamide liés à des atomes d'hydrogène ou à des atomes de carbone acycliques
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample and for stabilizing the transcriptome of contained cells.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C07C 233/05 - Amides d'acides carboxyliques ayant des atomes de carbone de groupes carboxamide liés à des atomes d'hydrogène ou à des atomes de carbone acycliques ayant les atomes d'azote des groupes carboxamide liés à des atomes d'hydrogène ou à des atomes de carbone de radicaux hydrocarbonés non substitués avec des atomes de carbone de groupes carboxamide liés à des atomes de carbone d'un squelette carboné saturé acyclique ayant les atomes d'azote des groupes carboxamide liés à des atomes d'hydrogène ou à des atomes de carbone acycliques
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for use
in industry or research use; chemical, biochemical and
biotechnological products for non-medical purposes;
chemical, biochemical and biotechnological products for use
in in vitro-diagnostic for non-medical purposes; chemical,
biochemical and biotechnological products for use in
scientific and laboratory research; chemical, biochemical
and biotechnological products for use in in-vitro diagnostic
for scientific research or laboratory use; kits comprising
chemical, biochemical and biotechnological products for
industrial and scientific purposes; kits comprising
chemical, biochemical and biotechnological products for use
in in-vitro diagnostic for non-medical purposes; non-medical
reagents, enzymes and chemicals, used in analytical
chemistry, for scientific and/or medical research use for
marking, separating, isolating and/or for the analysis of
biopolymers, in particular nucleic acids, in particular
reagents, enzymes and buffer solutions for nucleic acid
isolation and analysis; chemical test kits for laboratory or
research use for marking, separating, isolating and/or for
the analysis of biopolymers, in particular nucleic acids, in
particular comprising reagents, enzymes and buffer solutions
for nucleic acid isolation and analysis. Chemical, biochemical and biotechnological products for
medical and veterinary purposes; chemical, biochemical and
biotechnological products for human in-vitro diagnostic
purposes; chemical, biochemical and biotechnological
reagents and preparations for human in-vitro diagnostic
purposes; kits comprising chemical, biochemical and
biotechnological products for human in vitro diagnostic;
chemical, biochemical and biotechnological products for
medical and pharmaceutical research; kits comprising
chemical products for human in vitro diagnostic for medical
or pharmaceutical purposes; kits comprising chemical,
biochemical and biotechnological products for human in vitro
diagnostic for detection of pathogens, viruses and bacteria
for medical or pharmaceutical purposes; diagnostic
preparations for medical and veterinary purposes, namely
diagnostics preparations for marking, separating, isolating
and/or for the analysis of biopolymers, namely nucleic
acids; diagnostic reagents, enzymes and buffer solutions for
nucleic acid isolation and analysis for medical or
veterinary purposes; chemical, biochemical and
biotechnological diagnostic preparations for medical and
veterinary purposes, namely reagents, enzymes and buffer
solutions for marking, separating, isolating and/or for the
analysis of biopolymers, namely nucleic acids; diagnostic
kits containing preparations for medical and veterinary
diagnostic purposes, namely for marking, separating,
isolating and/or for the analysis of biopolymers, namely
nucleic acids; diagnostic kits containing reagents, enzymes
and buffer solutions for nucleic acid isolation,
amplification and analysis for medical or veterinary
purposes. Laboratory apparatus and instruments; scientific
apparatuses, instruments and devices, including from
recycled material, as well as their parts and components
included in this class; tubes, pipette tips, chromatographic
columns, including tubes, pipette tips and chromatographic
columns from recycled material; hollow glassware for
laboratory use; glassware for scientific use [specifically
adapted]; hollow glass containers for measuring; laboratory
storage tubes, including from recycled material; holders for
test tubes, including from recycled material; disposable
dispenser syringes for laboratory use, including from
recycled material; software for use in scientific and
medical research; scientific apparatuses, instruments and
devices, in particular in the area of analysis of nucleic
acid; laboratory filters; ultra filtration membranes for
laboratory apparatus; filters for biological samples,
including blood and blood components for laboratory use;
filters for biological samples, including blood and blood
components for laboratory experiments; laboratory instrument
for the detection of pathogens and toxins in a biological
sample for research use.
6.
METHOD FOR ISOLATING NUCLEIC ACIDS FROM AN INHIBITOR RICH BIOLOGICAL SAMPLE
The present invention provides a method for isolating nucleic acids from a sample, preferably a stool or gut sample, the method comprising (a) preparing a lysed sample, wherein lysate preparation comprises contacting the sample with (i) at least one chaotropic agent and preferably a phosphate, (ii) at least one RNase inhibiting agent, (iii) at least one protein precipitating agent, and (iv) at least one inhibitor removing agent, (b) clearing the lysate; (c) isolating nucleic acids from the liquid fraction of the cleared lysate. The method is particularly useful for isolating total nucleic acids, DNA or RNA from inhibitor- rich samples, such as stool samples. In contrast to prior art method, it advantageously omits a second inhibitor removal step after lysate clearance step (b) and prior to performing nucleic acid isolation step (b).
The present invention provides a method for recovering RNA from a sample, the method comprising (a) preparing a lysed sample, wherein lysate preparation comprises contacting the sample with (i) at least one chaotropic agent and preferably a phosphate, (ii) at least one RNase inhibiting agent, (iii) at least one protein precipitating agent, and (iv) at least one inhibitor removing agent, (b) clearing the lysate; (c) contacting the cleared lysate with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture; (d) obtaining a RNA containing liquid phase from the mixture; and (e) recovering RNA from the liquid phase. The method allows to isolate RNA with high purity and yield from inhibitor-rich samples, such as soil, stool and wastewater samples. Inhibitory contaminants are efficiently depleted with the method of the present invention.
The present invention relates to a combination of agents for improving nucleic acid processing, such as amplification or sequencing, a method for improving nucleic acid processing involving such combination of agents, a composition for use in such a method, and to a kit associated with such method.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Chemical, biochemical and biotechnological products for use in industry or research use; chemical, biochemical and biotechnological products for non-medical purposes; chemical, biochemical and biotechnological products for use in in vitro-diagnostic for non-medical purposes; chemical, biochemical and biotechnological products for use in scientific and laboratory research; chemical, biochemical and biotechnological products for use in in-vitro diagnostic for scientific research or laboratory use; kits comprising chemical, biochemical and biotechnological products for industrial and scientific purposes; kits comprising chemical, biochemical and biotechnological products for use in in-vitro diagnostic for non-medical purposes; non-medical reagents, enzymes and chemicals, used in analytical chemistry, for scientific and/or medical research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid isolation and analysis; chemical test kits for laboratory or research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular nucleic acids, in particular comprising reagents, enzymes and buffer solutions for nucleic acid isolation and analysis.
(2) Chemical, biochemical and biotechnological products for medical and veterinary purposes; chemical, biochemical and biotechnological products for human in-vitro diagnostic purposes; chemical, biochemical and biotechnological reagents and preparations for human in-vitro diagnostic purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic; chemical, biochemical and biotechnological products for medical and pharmaceutical research; kits comprising chemical products for human in vitro diagnostic for medical or pharmaceutical purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic for detection of pathogens, viruses and bacteria for medical or pharmaceutical purposes; diagnostic preparations for medical and veterinary purposes, namely diagnostics preparations for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic reagents, enzymes and buffer solutions for nucleic acid isolation and analysis for medical or veterinary purposes; chemical, biochemical and biotechnological diagnostic preparations for medical and veterinary purposes, namely reagents, enzymes and buffer solutions for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic kits containing preparations for medical and veterinary diagnostic purposes, namely for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic kits containing reagents, enzymes and buffer solutions for nucleic acid isolation, amplification and analysis for medical or veterinary purposes.
(3) Laboratory apparatus and instruments; scientific apparatuses, instruments and devices, including from recycled material, as well as their parts and components included in this class; tubes, pipette tips, chromatographic columns, including tubes, pipette tips and chromatographic columns from recycled material; hollow glassware for laboratory use; glassware for scientific use [specifically adapted]; hollow glass containers for measuring; laboratory storage tubes, including from recycled material; holders for test tubes, including from recycled material; disposable dispenser syringes for laboratory use, including from recycled material; software for use in scientific and medical research; scientific apparatuses, instruments and devices, in particular in the area of analysis of nucleic acid; laboratory filters; ultra filtration membranes for laboratory apparatus; filters for biological samples, including blood and blood components for laboratory use; filters for biological samples, including blood and blood components for laboratory experiments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for use in industry or research use; chemical, biochemical and biotechnological products for non-medical purposes; chemical, biochemical and biotechnological products for use in in vitro-diagnostic for non-medical purposes; chemical, biochemical and biotechnological products for use in scientific and laboratory research; chemical, biochemical and biotechnological products for use in in-vitro diagnostic for scientific research or laboratory use; kits comprising chemical, biochemical and biotechnological products for industrial and scientific purposes; kits comprising chemical, biochemical and biotechnological products for use in in-vitro diagnostic for non-medical purposes; non-medical reagents, enzymes and chemicals, used in analytical chemistry, for scientific and/or medical research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid isolation and analysis; chemical test kits for laboratory or research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular nucleic acids, in particular comprising reagents, enzymes and buffer solutions for nucleic acid isolation and analysis. Chemical, biochemical and biotechnological products for medical and veterinary purposes; chemical, biochemical and biotechnological products for human in-vitro diagnostic purposes; chemical, biochemical and biotechnological reagents and preparations for human in-vitro diagnostic purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic; chemical, biochemical and biotechnological products for medical and pharmaceutical research; kits comprising chemical products for human in vitro diagnostic for medical or pharmaceutical purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic for detection of pathogens, viruses and bacteria for medical or pharmaceutical purposes; diagnostic preparations for medical and veterinary purposes, namely diagnostics preparations for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic reagents, enzymes and buffer solutions for nucleic acid isolation and analysis for medical or veterinary purposes; chemical, biochemical and biotechnological diagnostic preparations for medical and veterinary purposes, namely reagents, enzymes and buffer solutions for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic kits containing preparations for medical and veterinary diagnostic purposes, namely for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic kits containing reagents, enzymes and buffer solutions for nucleic acid isolation, amplification and analysis for medical or veterinary purposes. Laboratory apparatus and instruments; scientific apparatuses, instruments and devices, including from recycled material, as well as their parts and components included in this class; tubes, pipette tips, chromatographic columns, including tubes, pipette tips and chromatographic columns from recycled material; hollow glassware for laboratory use; glassware for scientific use [specifically adapted]; hollow glass containers for measuring; laboratory storage tubes, including from recycled material; holders for test tubes, including from recycled material; disposable dispenser syringes for laboratory use, including from recycled material; software for use in scientific and medical research; scientific apparatuses, instruments and devices, in particular in the area of analysis of nucleic acid; laboratory filters; ultra filtration membranes for laboratory apparatus; filters for biological samples, including blood and blood components for laboratory use; filters for biological samples, including blood and blood components for laboratory experiments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use.
The present invention relates to a method for amplifying a target RNA in a sample, an oligonucleotide usable in the method according to the invention, a kit for amplifying a target RNA, and a use of an oligonucleotide for inhibiting an aptamer oligonucleotide.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
12.
SIGNAL NORMALIZATION OF NUCLEIC ACID AMPLIFICATION REACTION PRODUCTS
The invention relates to a multiplex nucleic acid amplification reaction comprising at least two separate amplicons, with normalized signal strength, wherein for at least one amplicon either the forward primer oligonucleotides, or the reverse primers oligonucleotides, or the optional detection probe oligonucleotides, are only partially labeled, and the subset amount of labeled primer/probe oligonucleotides to subset amount of unlabeled primer/probe oligonucleotides is adjusted so that the signal strength in both amplicons is equal or nearly equal. Kits and compositions are also claimed.
The invention relates to a method for examining a nucleic acid amplification product, comprising the steps of a. providing an amplification product stemming from a preferably exponential amplification comprising i. an upstream and downstream primer region as well as optionally and preferably, ii. an amplified target region between said upstream and downstream primer region, b. examining the amplification product by amplifying said amplification product in a PCR reaction, wherein i. the upstream and downstream PCR primers in said PCR reaction bind in the upstream and downstream primer region of the amplification product, ii. a first and a second oligonucleotide probe are placed downstream of the first and second PCR primers and within the upstream and the downstream primer region, c. performing a PCR reaction and examining the newly formed amplification product, wherein the upstream and downstream primer regions of step a.i.) have a length of under 35 nucleotides and over 20 nucleotides.
The present invention relates to a method of quantifying the amount of at least one nucleic acid target, the method comprising: a) Providing a defined number of cells, wherein the defined number of cells is at least one cell; and b) Lysing the at least one cell, thereby releasing RNA and DNA; and c) Performing a reverse transcription reaction on the released RNA, thereby generating complementary DNA (cDNA), and further performing a digital polymerase chain reaction (dPCR) on at least one reverse transcribed RNA target within the cDNA; and/or performing a digital polymerase chain reaction (dPCR) on at least one DNA target within the released DNA, wherein the dPCR is performed in a plate; and d) Quantifying the amount of the at least one nucleic acid target.
The invention refers to a method for signal collection in a process that utilizes fluorescence detection at multiple wavelengths, especially a qPCR process or dPCR process, using at least two channels, wherein for each of the at least two channels an excitation condition and a detection condition is provided for one of the multiple wavelengths, respectively, and wherein the method is a computer-implemented method and during the signal collection in the fluorescence detection: i) a first signal in the first channel is recorded; ii) a second signal in the second channel is recorded; and iii) a crosstalk correlation is calculated using a dependency between the first signal and the second signal of the fluorescence detection, wherein the dependency used between the first signal and the second signal is a linear dependency and a linear regression between the data corresponding to the signal of the two channels is carried out.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
G01F 11/00 - Appareils qu'il faut actionner de l'extérieur, adaptés à chaque opération répétée et identique, pour mesurer et séparer le volume prédéterminé d'un fluide ou d'un matériau solide fluent à partir d'une alimentation ou d'un récipient sans tenir compte du poids, et pour fournir ce volume
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
16.
HIGH STOKES SHIFT FLUORESCENCE DYES FOR MULTIPLEX DETECTION
The invention relates to the use of a compound, wherein the compound is a fluorescence dye with a high Stokes shift, which is large enough to skip at least one intermediate emission filter channel, i.e., to jump at least into the next but one channel, as reference dye for non-droplet based dPCR systems to evaluate filling levels of compartments, as reference dye for fluorescence PCR systems for normalization or as dye for target detection for multiplex PCR, wherein the compound is bound to a probe or primer as fluorescent label, wherein the PCR is selected from the group consisting of digital PCR (dPCR), quantitative PCR (qPCR), reverse transcription PCR (RT-PCR), reverse transcription quantitative PCR (RT-qPCR) and real-time PCR.
The present disclosure provides methods and kits for stabilizing extracellular nucleic acids in a cell-depleted biological sample obtained from a fluid cell-containing biological sample, which preferably is a urine sample.
The present disclosure relates to methods and kits for treatment of biological samples comprising non-sperm cells and sperm cells. The present disclosure relates more particularly to methods and kits for treatment of forensic samples comprising non-sperm cells and sperm cells. Moreover, the present disclosure relates to the use of specific enzymes for the decomposition of non-sperm DNA in a sample comprising a mixture of non-sperm DNA and sperm DNA, the sperm DNA being contained in sperm cells and/or sperm nuclei, and a corresponding method, therefore.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for use in industry or research use; chemical, biochemical and biotechnological products for non-medical purposes; chemical, biochemical and biotechnological products for use in in vitro-diagnostic for non-medical purposes; chemical, biochemical and biotechnological products for use in scientific and laboratory research; chemical, biochemical and biotechnological products for use in in-vitro diagnostic for scientific research or laboratory use; chemical, biochemical and biotechnological reagents and preparations for use in scientific and laboratory research; kits comprising chemical, biochemical and biotechnological products for industrial and scientific purposes; kits comprising chemical, biochemical and biotechnological products for use in in-vitro diagnostic for non-medical purposes; non-medical reagents, enzymes and chemicals, used in analytical chemistry, for scientific and/or medical research use for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and their molecular constituents like proteins, lipids and nucleic acids, including DNA, RNA, mRNA, miRNA, in particular reagents, enzymes and buffer solutions for purification, isolation and analysis of extracellular vesicles and nucleic acids; chemical test kits for laboratory or research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and nucleic acids, in particular comprising reagents, enzymes and buffer solutions for analysis. Chemical, biochemical and biotechnological products for medical and veterinary purposes; chemical, biochemical and biotechnological products for human in-vitro diagnostic purposes; chemical, biochemical and biotechnological reagents and preparations for human in-vitro diagnostic purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic; chemical , biochemical and biotechnological products for medical and pharmaceutical research; kits comprising chemical products for human in-vitro diagnostic for medical or pharmaceutical purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic for detection of pathogens, viruses and bacteria for medical or pharmaceutical purposes; diagnostic preparations for medical and veterinary purposes, namely diagnostics preparations for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and their molecular constituents like proteins, lipids and nucleic acids, including DNA, RNA, mRNA, miRNA; diagnostic reagents, enzymes and buffer solutions for purification isolation and analysis of extracellular vesicles and nucleic acid for medical or veterinary purposes; chemical, biochemical and biotechnological diagnostic preparations for medical and veterinary purposes, in particular reagents, enzymes and buffer solutions for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles and their molecular constituents like nucleic acids, proteins and lipids; diagnostic kits containing preparations for medical and veterinary diagnostic purposes, in particular for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles , nucleic acids, including DNA, RNA, mRNA, miRNA, proteins and lipids; diagnostic kits containing reagents, enzymes and buffer solutions for purification and isolation of extracellular vesicles and their molecular constituents and nucleic acid isolation, amplification and analysis for medical or veterinary purposes. Laboratory apparatus and instruments; Scientific apparatuses, instruments and devices, as well as their parts and components included in this class; Tubes, pipette tips, chromatographic columns; spin columns; membranes; spin column membranes; Bottles and vessels for laboratory use; Laboratory storage and collecting tubes; Holders for test tubes; Software for use in scientific and medical research; Scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, in particular for use in the purification, isolation and/or analysis of nucleic acids, including DNA, RNA, mRNA, miRNA, and extracellular vesicles, such as exosomes, and their molecular constituents like proteins, nucleic acids and lipids; Scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, for use in analysis and determination of quality and quantity of vesicles; Laboratory filters; Membranes including ultrafiltration and adsorption membranes, for laboratory apparatus; Filters for biological samples, including cell cultures and their supernatants, blood and blood components for laboratory use; scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, in particular for use in purification, isolation and/ or analysis of vesicles from biological samples, in particular biofluids, including urine, serum, plasma, cerebrospinal fluid (CSF) and cell culture supernatant.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Diagnostic preparations for medical or veterinary purposes;
diagnostic preparations for humans for medical purposes;
preparations for medical and veterinary purposes, in
particular diagnostics, for marking, amplifying,
quantification and/or for the analysis of biopolymers, in
particular nucleic acids; chemical, biochemical and
biotechnological preparations for medical and veterinary
purposes, in particular reagents, enzymes and buffer
solutions for marking, amplifying, quantification and/or for
the analysis of biopolymers, in particular nucleic acids, in
particular reagents, enzymes and buffer solutions for
nucleic acid amplification; kits containing preparations for
medical and veterinary diagnostic purposes, in particular
for marking, amplifying, quantification and/or for the
analysis of biopolymers, in particular nucleic acids, in
particular reagents, enzymes and buffer solutions for
nucleic acid amplification; chemical reagents for medical
purposes; cartridges containing medical diagnostic reagents
for use in Polymerase Chain Reaction (PCR) or digital
Polymerase Chain Reaction (PCR) instruments; medical
diagnostic assays for testing of body samples, including
bodily fluids and tissue in the detection, monitoring, and
prediction of disease or other conditions, disease states,
hormone level, drug level, drug response, organ function
effectiveness, and clinical response; diagnostic test kits
comprised of medical diagnostic reagents and assays for
testing in the field of medical diagnostics. Apparatus for medical and veterinary diagnostic analysis and
determination of medical and veterinary conditions, namely
DNA and RNA testing apparatus; Medical and veterinary
equipment for diagnostic purposes; clinical medical
laboratory apparatus and analytical instruments, namely
digital Polymerase Chain Reaction (d PCR) instruments for
use in in-vitro and in-vivo diagnostics; medical devices for
detection of infectious disease, namely, digital polymerase
chain reaction (dPCR) platforms; medical devices for use in
pre-natal screenings, namely, digital polymerase chain
reaction (dPCR) platform; medical devices, namely, digital
polymerase chain reaction (dPCR) instruments for use in
detecting, diagnosing and treating cancer and infectious
disease, namely, digital polymerase chain reaction (dPCR)
platform; medical devices, namely, digital polymerase chain
reaction (dPCR) instruments for use in pre-natal screenings,
namely, digital polymerase chain reaction (PCR) platform;
medical devices, namely, instruments for use in measuring
and quantifying copy number of DNA for medical diagnostic
purposes, namely, digital polymerase chain reaction (dPCR)
platform; microfluidic plates for use as medical apparatus. Testing services, namely, quantifying DNA and RNA for
medical, diagnostic and treatment purposes; rental of
digital Polymerase Chain Reaction (PCR) apparatus; RNA or
DNA analysis for cancer diagnosis and prognosis; Medical
services, in particular medical analysis in connection with
the treatment of individuals; providing information in the
fields of medicine, veterinary medicine and pharmaceuticals;
health centers, namely, genetic centers providing medical
diagnostic, preventive and curative therapy services for
genetic disorders, cancer treatment centers, rare disease
treatment centers, children's hospitals and women's
hospital; medical clinics; gene therapy services in the
field of oncology, infertility, prenatal diagnostics,
carrier testing, and genetically inherited diseases; medical
laboratory services for the analysis of samples taken from
patients.
21.
METHOD FOR ISOLATING NUCLEIC ACIDS FROM A FORMALDEHYDE RELEASER STABILIZED SAMPLE
The present invention pertains to a method for isolation and purification of nucleic acids from a stabilized sample or portion or fraction thereof, wherein the sample stabilization involved the use of at least one formaldehyde releaser and wherein the isolation of the nucleic acids from the stabilized sample or portion or fraction thereof involves the use of at least one cationic detergent during lysis.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Diagnostic preparations for medical or veterinary purposes; diagnostic preparations for humans for medical purposes; preparations for medical and veterinary purposes, in particular diagnostics, for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids; chemical, biochemical and biotechnological preparations for medical and veterinary purposes, in particular reagents, enzymes and buffer solutions for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; kits containing preparations for medical and veterinary diagnostic purposes, in particular for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; chemical reagents for medical purposes; cartridges containing medical diagnostic reagents for use in Polymerase Chain Reaction (PCR) or digital Polymerase Chain Reaction (PCR) instruments; medical diagnostic assays for testing of body samples, including bodily fluids and tissue in the detection, monitoring, and prediction of disease or other conditions, disease states, hormone level, drug level, drug response, organ function effectiveness, and clinical response; diagnostic test kits comprised of medical diagnostic reagents and assays for testing in the field of medical diagnostics. Apparatus for medical and veterinary diagnostic analysis and determination of medical and veterinary conditions, namely DNA and RNA testing apparatus; Medical and veterinary equipment for diagnostic purposes; clinical medical laboratory apparatus and analytical instruments, namely digital Polymerase Chain Reaction (d PCR) instruments for use in in-vitro and in-vivo diagnostics; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (dPCR) platforms; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (dPCR) platform; microfluidic plates for use as medical apparatus. Testing services, namely, quantifying DNA and RNA for medical, diagnostic and treatment purposes; rental of digital Polymerase Chain Reaction (PCR) apparatus; RNA or DNA analysis for cancer diagnosis and prognosis; Medical services, in particular medical analysis in connection with the treatment of individuals; providing information in the fields of medicine, veterinary medicine and pharmaceuticals; health centers, namely, genetic centers providing medical diagnostic, preventive and curative therapy services for genetic disorders, cancer treatment centers, rare disease treatment centers, children's hospitals and women's hospital; medical clinics; gene therapy services in the field of oncology, infertility, prenatal diagnostics, carrier testing, and genetically inherited diseases; medical laboratory services for the analysis of samples taken from patients.
A microfluidic system is presented that includes a cartridge, a container, and a lid for the container. The cartridge includes a plurality of microfluidic channels coupled to one or more chambers. The container holds dry chemicals and includes a housing with a first opening and a second opening smaller than the first opening. The container is designed to be inserted into an opening of the cartridge, such that the container is independently secured within the opening. The insertion of the container allows for the container to be fluidically coupled with a microfluidic channel of the plurality of microfluidic channels via the second opening. The lid includes a column that extends from the lid into the container.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Diagnostic preparations for medical or veterinary purposes;
diagnostic preparations for humans for medical purposes;
preparations for medical and veterinary purposes, in
particular diagnostics, for marking, amplifying,
quantification and/or for the analysis of biopolymers, in
particular nucleic acids; chemical, biochemical and
biotechnological preparations for medical and veterinary
purposes, in particular reagents, enzymes and buffer
solutions for marking, amplifying, quantification and/or for
the analysis of biopolymers, in particular nucleic acids, in
particular reagents, enzymes and buffer solutions for
nucleic acid amplification; kits containing preparations for
medical and veterinary diagnostic purposes, in particular
for marking, amplifying, quantification and/or for the
analysis of biopolymers, in particular nucleic acids, in
particular reagents, enzymes and buffer solutions for
nucleic acid amplification; chemical reagents for medical
purposes; cartridges containing medical diagnostic reagents
for use in Polymerase Chain Reaction (PCR) or digital
Polymerase Chain Reaction (PCR) instruments; medical
diagnostic assays for testing of body samples, including
bodily fluids and tissue in the detection, monitoring, and
prediction of disease or other conditions, disease states,
hormone level, drug level, drug response, organ function
effectiveness, and clinical response; diagnostic test kits
comprised of medical diagnostic reagents and assays for
testing in the field of medical diagnostics. Apparatus for medical and veterinary diagnostic analysis and
determination of medical and veterinary conditions, namely
DNA and RNA testing apparatus; Medical and veterinary
equipment for diagnostic purposes; clinical medical
laboratory apparatus and analytical instruments, namely
digital Polymerase Chain Reaction (d PCR) instruments for
use in in-vitro and in-vivo diagnostics; medical devices for
detection of infectious disease, namely, digital polymerase
chain reaction (dPCR) platforms; medical devices for use in
pre-natal screenings, namely, digital polymerase chain
reaction (dPCR) platform; medical devices, namely, digital
polymerase chain reaction (dPCR) instruments for use in
detecting, diagnosing and treating cancer and infectious
disease, namely, digital polymerase chain reaction (dPCR)
platform; medical devices, namely, digital polymerase chain
reaction (dPCR) instruments for use in pre-natal screenings,
namely, digital polymerase chain reaction (PCR) platform;
medical devices, namely, instruments for use in measuring
and quantifying copy number of DNA for medical diagnostic
purposes, namely, digital polymerase chain reaction (dPCR)
platform; microfluidic plates for use as medical apparatus. Testing services, namely, quantifying DNA and RNA for
medical, diagnostic and treatment purposes; rental of
digital Polymerase Chain Reaction (PCR) apparatus; RNA or
DNA analysis for cancer diagnosis and prognosis; Medical
services, in particular medical analysis in connection with
the treatment of individuals; providing information in the
fields of medicine, veterinary medicine and pharmaceuticals;
health centers, namely, genetic centers providing medical
diagnostic, preventive and curative therapy services for
genetic disorders, cancer treatment centers, rare disease
treatment centers, children's hospitals and women's
hospital; medical clinics; gene therapy services in the
field of oncology, infertility, prenatal diagnostics,
carrier testing, and genetically inherited diseases; medical
laboratory services for the analysis of samples taken from
patients.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for use in industry or research use; chemical, biochemical and biotechnological products for non-medical purposes; chemical, biochemical and biotechnological products for use in in vitro-diagnostic for non-medical purposes; chemical, biochemical and biotechnological products for use in scientific and laboratory research; chemical, biochemical and biotechnological products for use in in-vitro diagnostic for scientific research or laboratory use; chemical, biochemical and biotechnological reagents and preparations for use in scientific and laboratory research; kits comprising chemical, biochemical and biotechnological products for industrial and scientific purposes; kits comprising chemical, biochemical and biotechnological products for use in in-vitro diagnostic for non-medical purposes; non-medical reagents, enzymes and chemicals, used in analytical chemistry, for scientific and/or medical research use for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and their molecular constituents like proteins, lipids and nucleic acids, including DNA, RNA, mRNA, miRNA, in particular reagents, enzymes and buffer solutions for purification, isolation and analysis of extracellular vesicles and nucleic acids; chemical test kits for laboratory or research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and nucleic acids, in particular comprising reagents, enzymes and buffer solutions for analysis. Chemical, biochemical and biotechnological products for medical and veterinary purposes; chemical, biochemical and biotechnological products for human in-vitro diagnostic purposes; chemical, biochemical and biotechnological reagents and preparations for human in-vitro diagnostic purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic; chemical , biochemical and biotechnological products for medical and pharmaceutical research; kits comprising chemical products for human in-vitro diagnostic for medical or pharmaceutical purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic for detection of pathogens, viruses and bacteria for medical or pharmaceutical purposes; diagnostic preparations for medical and veterinary purposes, namely diagnostics preparations for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and their molecular constituents like proteins, lipids and nucleic acids, including DNA, RNA, mRNA, miRNA; diagnostic reagents, enzymes and buffer solutions for purification isolation and analysis of extracellular vesicles and nucleic acid for medical or veterinary purposes; chemical, biochemical and biotechnological diagnostic preparations for medical and veterinary purposes, in particular reagents, enzymes and buffer solutions for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles and their molecular constituents like nucleic acids, proteins and lipids; diagnostic kits containing preparations for medical and veterinary diagnostic purposes, in particular for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles , nucleic acids, including DNA, RNA, mRNA, miRNA, proteins and lipids; diagnostic kits containing reagents, enzymes and buffer solutions for purification and isolation of extracellular vesicles and their molecular constituents and nucleic acid isolation, amplification and analysis for medical or veterinary purposes. Laboratory apparatus and instruments; Scientific apparatuses, instruments and devices, as well as their parts and components included in this class; Tubes, pipette tips, chromatographic columns; spin columns; membranes; spin column membranes; Bottles and vessels for laboratory use; Laboratory storage and collecting tubes; Holders for test tubes; Software for use in scientific and medical research; Scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, in particular for use in the purification, isolation and/or analysis of nucleic acids, including DNA, RNA, mRNA, miRNA, and extracellular vesicles, such as exosomes, and their molecular constituents like proteins, nucleic acids and lipids; Scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, for use in analysis and determination of quality and quantity of vesicles; ,Laboratory filters; Membranes including ultrafiltration and adsorption membranes, for laboratory apparatus; Filters for biological samples, including cell cultures and their supernatants, blood and blood components for laboratory use; scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, in particular for use in purification, isolation and/ or analysis of vesicles from biological samples, in particular biofluids, including urine, serum, plasma, cerebrospinal fluid (CSF) and cell culture supernatant.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for use in industry or research use; chemical, biochemical and biotechnological products for non-medical purposes; chemical, biochemical and biotechnological products for use in in vitro-diagnostic for non-medical purposes; chemical, biochemical and biotechnological products for use in scientific and laboratory research; chemical, biochemical and biotechnological products for use in in-vitro diagnostic for scientific research or laboratory use; chemical, biochemical and biotechnological reagents and preparations for use in scientific and laboratory research; kits comprising chemical, biochemical and biotechnological products for industrial and scientific purposes; kits comprising chemical, biochemical and biotechnological products for use in in-vitro diagnostic for non-medical purposes; non-medical reagents, enzymes and chemicals, used in analytical chemistry, for scientific and/or medical research use for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and their molecular constituents like proteins, lipids and nucleic acids, including DNA, RNA, mRNA, miRNA, in particular reagents, enzymes and buffer solutions for purification, isolation and analysis of extracellular vesicles and nucleic acids; chemical test kits for laboratory or research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and nucleic acids, in particular comprising reagents, enzymes and buffer solutions for analysis. Chemical, biochemical and biotechnological products for medical and veterinary purposes; chemical, biochemical and biotechnological products for human in-vitro diagnostic purposes; chemical, biochemical and biotechnological reagents and preparations for human in-vitro diagnostic purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic; chemical , biochemical and biotechnological products for medical and pharmaceutical research; kits comprising chemical products for human in-vitro diagnostic for medical or pharmaceutical purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic for detection of pathogens, viruses and bacteria for medical or pharmaceutical purposes; diagnostic preparations for medical and veterinary purposes, namely diagnostics preparations for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles such as exosomes and their molecular constituents like proteins, lipids and nucleic acids, including DNA, RNA, mRNA, miRNA; diagnostic reagents, enzymes and buffer solutions for purification isolation and analysis of extracellular vesicles and nucleic acid for medical or veterinary purposes; chemical, biochemical and biotechnological diagnostic preparations for medical and veterinary purposes, in particular reagents, enzymes and buffer solutions for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles and their molecular constituents like nucleic acids, proteins and lipids; diagnostic kits containing preparations for medical and veterinary diagnostic purposes, in particular for marking, separating, isolating, purification and/or for the analysis of biopolymers, in particular extracellular vesicles , nucleic acids, including DNA, RNA, mRNA, miRNA, proteins and lipids; diagnostic kits containing reagents, enzymes and buffer solutions for purification and isolation of extracellular vesicles and their molecular constituents and nucleic acid isolation, amplification and analysis for medical or veterinary purposes. Laboratory apparatus and instruments; Scientific apparatuses, instruments and devices, as well as their parts and components included in this class; Tubes, pipette tips, chromatographic columns; spin columns; membranes; spin column membranes; Bottles and vessels for laboratory use; Laboratory storage and collecting tubes; Holders for test tubes; Software for use in scientific and medical research; Scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, in particular for use in the purification, isolation and/or analysis of nucleic acids, including DNA, RNA, mRNA, miRNA, and extracellular vesicles, such as exosomes, and their molecular constituents like proteins, nucleic acids and lipids; Scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, for use in analysis and determination of quality and quantity of vesicles; Laboratory filters; Membranes including ultrafiltration and adsorption membranes, for laboratory apparatus; Filters for biological samples, including cell cultures and their supernatants, blood and blood components for laboratory use; scientific apparatuses, instruments and devices, including disposable equipment, components and parts thereof, in particular for use in purification, isolation and/ or analysis of vesicles from biological samples, in particular biofluids, including urine, serum, plasma, cerebrospinal fluid (CSF) and cell culture supernatant.
27.
METHOD FOR PROVIDING AN ANALYSIS OF PARTITION IMAGE DATA
The invention relates to a method (200) for providing an analysis of partition image data (142), in particular for providing an analysis of a plurality of partition states of at least one microfluidic well (134) within a digital polymerase chain reaction analysis. The method comprising the steps - receiving the partition image data which is indicating an optical appearance of a plurality of partitions (132) that are arranged in a preexisting grid structure (365); - calculating a matrix correlation function R(x,y) for each point (x,y) of the at least one microfluidic well depicted within the partition image data, wherein the matrix correlation function R(x,y) indicates a correlation between a predefined template partition matrix T(x',y') and the at least one depicted microfluidic well; - calculating a set of assumed grid point locations (360) within the partition image data based on a predefined number of expected grid points and on the calculated matrix correlation function R(x,y); - detecting a respective partition state based on an optical appearance of the calculated set of assumed grid point locations within the partition image data for each partition of the at least one microfluidic well; and - providing analysis information (125) based on the detected partition states.
The present disclosure relates to a method of evaluating a mutational burden in a sample. The method includes providing first data which represents allelic depths of the sample; calculating rate data based on the first data; segmenting the rate data into a plurality of segments of rate data using a wavelet transform; estimating parameters for each of the plurality of segments; classifying each of the plurality of segments based on the estimated parameters. The classifying includes, for each of a plurality of classification thresholds, generating a classification for the plurality of segments based on the classification threshold and determining an intermediate value based on the classification. The method further comprises determining a mutational burden of the sample based on the intermediate values.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for use in industry or research use; chemical, biochemical and biotechnological products for non-medical purposes; chemical, biochemical and biotechnological products for use in in vitro-diagnostic for non-medical purposes; chemical, biochemical and biotechnological products for use in scientific and laboratory research; chemical, biochemical and biotechnological products for use in in-vitro diagnostic for scientific research or laboratory use; kits comprising chemical, biochemical and biotechnological products for industrial and scientific purposes; kits comprising chemical, biochemical and biotechnological products for use in in-vitro diagnostic for non-medical purposes; non-medical reagents, enzymes and chemicals, used in analytical chemistry, for scientific and/or medical research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid isolation and analysis; chemical test kits for laboratory or research use for marking, separating, isolating and/or for the analysis of biopolymers, in particular nucleic acids, in particular comprising reagents, enzymes and buffer solutions for nucleic acid isolation and analysis. Chemical, biochemical and biotechnological products for medical and veterinary purposes; chemical, biochemical and biotechnological products for human in-vitro diagnostic purposes; chemical, biochemical and biotechnological reagents and preparations for human in-vitro diagnostic purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic; chemical , biochemical and biotechnological products for medical and pharmaceutical research; kits comprising chemical products for human in vitro diagnostic for medical or pharmaceutical purposes; kits comprising chemical, biochemical and biotechnological products for human in vitro diagnostic for detection of pathogens, viruses and bacteria for medical or pharmaceutical purposes; diagnostic preparations for medical and veterinary purposes, namely diagnostics preparations for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic reagents, enzymes and buffer solutions for nucleic acid isolation and analysis for medical or veterinary purposes; chemical, biochemical and biotechnological diagnostic preparations for medical and veterinary purposes, namely reagents, enzymes and buffer solutions for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic kits containing preparations for medical and veterinary diagnostic purposes, namely for marking, separating, isolating and/or for the analysis of biopolymers, namely nucleic acids; diagnostic kits containing reagents, enzymes and buffer solutions for nucleic acid isolation, amplification and analysis for medical or veterinary purposes. Laboratory apparatus and instruments; Scientific apparatuses, instruments and devices, including from recycled material, as well as their parts and components included in this class; tubes, pipette tips, chromatographic columns, including tubes, pipette tips and chromatographic columns from recycled material; Hollow glassware for laboratory use; Glassware for scientific use [specifically adapted]; Hollow glass containers for measuring; Laboratory storage tubes, including from recycled material; Holders for test tubes, including from recycled material; Disposable dispenser syringes for laboratory use, including from recycled material; Software for use in scientific and medical research; Scientific apparatuses, instruments and devices, in particular in the area of analysis of nucleic acid; Laboratory filters; Ultra filtration membranes for laboratory apparatus; Filters for biological samples, including blood and blood components for laboratory use; Filters for biological samples, including blood and blood components for laboratory experiments; Laboratory instrument for the detection of pathogens and toxins in a biological sample for research use.
30.
METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS USING ANION EXCHANGE PARTICLES
The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.
The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using an apoptosis inhibitor, preferably a caspase inhibitor, a hypertonic agent and/or a compound according to formula 1 as defined in the claims.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
The present invention provides methods and kits for isolating cell-free, non-vesicular miRNAs from a biological sample, such as serum and plasma, by using an acidic binding buffer comprising a buffering agent to promote binding of non-vesicular miRNAs to a solid phase comprising anion exchange groups. Furthermore, binding of non-vesicular miRNAs to the solid phase may be improved due to the presence of a crowding agent and by increasing the surface charge density of the solid phase.
The present invention provides methods for visualization, reviewing and manipulating of dPCR data. Furthermore, the present invention provides systems for dPCR data visualization, reviewing and manipulating and computer-readable storage media with instructions for dPCR data visualization, reviewing and manipulating. In addition, the use of such systems and computer-readable storage media for dPCR data visualization, reviewing and manipulating is provided.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Diagnostic preparations for medical or veterinary purposes; diagnostic preparations for humans for medical purposes; preparations for medical and veterinary purposes, in particular diagnostics, for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids; chemical, biochemical and biotechnological preparations for medical and veterinary purposes, in particular reagents, enzymes and buffer solutions for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; kits containing preparations for medical and veterinary diagnostic purposes, in particular for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; chemical reagents for medical purposes; cartridges containing medical diagnostic reagents for use in Polymerase Chain Reaction (PCR) or digital Polymerase Chain Reaction (PCR) instruments; medical diagnostic assays for testing of body samples, including bodily fluids and tissue in the detection, monitoring, and prediction of disease or other conditions, disease states, hormone level, drug level, drug response, organ function effectiveness, and clinical response; diagnostic test kits comprised of medical diagnostic reagents and assays for testing in the field of medical diagnostics. Apparatus for medical and veterinary diagnostic analysis and determination of medical and veterinary conditions, namely DNA and RNA testing apparatus; Medical and veterinary equipment for diagnostic purposes; clinical medical laboratory apparatus and analytical instruments, namely digital Polymerase Chain Reaction (d PCR) instruments for use in in-vitro and in-vivo diagnostics; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (dPCR) platforms; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (dPCR) platform; microfluidic plates for use as medical apparatus. Testing services, namely, quantifying DNA and RNA for medical, diagnostic and treatment purposes; rental of digital Polymerase Chain Reaction (PCR) apparatus; RNA or DNA analysis for cancer diagnosis and prognosis; Medical services, in particular medical analysis in connection with the treatment of individuals; providing information in the fields of medicine, veterinary medicine and pharmaceuticals; health centers, namely, genetic centers providing medical diagnostic, preventive and curative therapy services for genetic disorders, cancer treatment centers, rare disease treatment centers, children's hospitals and women's hospital; medical clinics; gene therapy services in the field of oncology, infertility, prenatal diagnostics, carrier testing, and genetically inherited diseases; medical laboratory services for the analysis of samples taken from patients.
35.
METHOD OF NUCLEIC ACID QUANTIFICATION USED TO INCREASE DYNAMIC RANGE IN DIGITAL PCR
The present invention relates to a method of nucleic acid quantification, the method comprising the steps of (i) providing a sample comprising a plurality of nucleic acid molecules; (ii) amplifying at least two loci within said plurality of nucleic acid molecules, wherein the at least two loci comprise at least one multicopy locus and at least one single copy locus and wherein the amplification method is a digital polymerase chain reaction method; (iii) detecting the at least two amplification products; and (iv) quantifying the amount of the plurality of nucleic acid molecules present in the sample; wherein the quantifiable nucleic acid concentration ranges for the at least one multicopy locus and the at least one single copy locus are overlapping. The invention also relates to a kit for use in the methods according to the invention.
The present invention relates to a new agent to modulate nucleic acid amplification by a polymerase with strand displacement activity, a method of amplifying nucleic acid by a polymerase with strand displacement activity involving said agent, a method of preparing a nucleic acid sequencing library, a method of sequencing a nucleic acid, and a kit for am- plifying nucleic acid amplification by a polymerase with strand displacement activity.
The present invention relates to a method for assessing the status of nucleic acid degradation and/or integrity of one or more nucleic acids in a sample, comprising the steps of digitally amplifying at least two regions within at least one locus, and detecting the amount of the at least two amplification products through the use of at least two probes. In the present invention preferably a nanoplate based dPCR method is applied.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Diagnostic preparations for medical or veterinary purposes; diagnostic preparations for humans for medical purposes; preparations for medical and veterinary purposes, in particular diagnostics, for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids; chemical, biochemical and biotechnological preparations for medical and veterinary purposes, in particular reagents, enzymes and buffer solutions for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; kits containing preparations for medical and veterinary diagnostic purposes, in particular for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; chemical reagents for medical purposes; cartridges containing medical diagnostic reagents for use in Polymerase Chain Reaction (PCR) or digital Polymerase Chain Reaction (PCR) instruments; medical diagnostic assays for testing of body samples, including bodily fluids and tissue in the detection, monitoring, and prediction of disease or other conditions, disease states, hormone level, drug level, drug response, organ function effectiveness, and clinical response; diagnostic test kits comprised of medical diagnostic reagents and assays for testing in the field of medical diagnostics. Apparatus for medical and veterinary diagnostic analysis and determination of medical and veterinary conditions, namely DNA and RNA testing apparatus; Medical and veterinary equipment for diagnostic purposes; clinical medical laboratory apparatus and analytical instruments, namely digital Polymerase Chain Reaction (d PCR) instruments for use in in-vitro and in-vivo diagnostics; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (dPCR) platforms; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (dPCR) platform; microfluidic plates for use as medical apparatus. Testing services, namely, quantifying DNA and RNA for medical, diagnostic and treatment purposes; rental of digital Polymerase Chain Reaction (PCR) apparatus; RNA or DNA analysis for cancer diagnosis and prognosis; Medical services, in particular medical analysis in connection with the treatment of individuals; providing information in the fields of medicine, veterinary medicine and pharmaceuticals; health centers, namely, genetic centers providing medical diagnostic, preventive and curative therapy services for genetic disorders, cancer treatment centers, rare disease treatment centers, children's hospitals and women's hospital; medical clinics; gene therapy services in the field of oncology, infertility, prenatal diagnostics, carrier testing, and genetically inherited diseases; medical laboratory services for the analysis of samples taken from patients.
39.
DIGITAL AMPLIFICATION METHOD FOR QUANTIFYING AND/OR DETERMINING INTEGRITY OF HUMAN MALE DNA
According to a first aspect of the present invention, a digital amplification method is provided for detecting and quantifying DNA and for determining degradation and/or integrity of the DNA, in particular male DNA in a sample, wherein the method comprises the step of digital amplification of at least two regions on at least one locus within the Y-chromosome.
METHOD FOR DETERMINING NUCLEIC ACID DEGRADATION IN A SAMPLE IN WHICH AT LEAST TWO OVERLAPPING AMPLICONS ARE PRODUCED AND TWO PROBES ARE USED IN THE METHOD
A method for assessing the status of degradation and/or integrity of one or more nucleic acids in a sample, which includes amplifying at least two overlapping regions within at least one locus and detecting the amount of the amplification products using at least two probes, where one probe binds to an overlapping region and the other probe binds to a non-overlapping region. Also, a method of designing primers and/or probes for amplifying at least two overlapping regions within at least one single copy locus or multicopy locus. Also a primer and a primer pair for amplifying at least two overlapping regions from one nucleic acid template which is a multicopy locus present in loci in the human genome. Also a kit including primers and/or probes for assessing the status of degradation and/or integrity of one or more nucleic acids in a sample.
Oligonucleotide primers and probes for quantifying and/or detecting human male DNA in a forensic sample. The oligonucleotides are useful for amplifying a multicopy locus within the human Y-chromosome (MCL-Y) that shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs. The oligonucleotides hybridize under stringent conditions to a nucleic acid having at least one sequence selected from the group consisting of SEQ ID NO. 3 to SEQ ID NO. 11 and/or SEQ ID NO. 17 to SEQ ID NO. 25. Kits including the oligonucleotide primers, probes and/or primer pairs and reagents for performing an amplification reaction on DNA recovered from a forensic sample are also disclosed.
The present invention relates to a lyophilized nucleic acid amplification composition comprising, i) a first reagent mixture for performing a nucleic acid amplification or elongation reaction, and ii) an amplification/elongation adjuvant preferably selected from the group of sucrose, sorbitol, methylglucosides, glycine, arginine, proline, erythritol, xylitol, maltose, lactose, Ficoll, mannitol, dextran, and trehalose. The invention also relates to method of amplifying a nucleic acid sample comprising the steps of, a) providing a lyophilized first mixture composition comprising, i) reagents for performing a nucleic acid amplification reaction, ii) and an amplification adjuvant selected from the group of sucrose, sorbitol, methylglucosides, glycine, arginine, proline, erythritol, xylitol, maltose, lactose, Ficoll, mannitol, dextran and trehalose, b) re-hydrating the lyophilization cakes, c) optionally adding one or more oligonucleotide primers depending on whether or not these are present in the first mixture, d) adding a sample, and e) performing an amplification reaction.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Diagnostic preparations for medical or veterinary purposes; diagnostic preparations for humans for medical purposes; preparations for medical and veterinary purposes, in particular diagnostics, for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids; chemical, biochemical and biotechnological preparations for medical and veterinary purposes, in particular reagents, enzymes and buffer solutions for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; kits containing preparations for medical and veterinary diagnostic purposes, in particular for marking, amplifying, quantification and/or for the analysis of biopolymers, in particular nucleic acids, in particular reagents, enzymes and buffer solutions for nucleic acid amplification; chemical reagents for medical purposes; cartridges containing medical diagnostic reagents for use in Polymerase Chain Reaction (PCR) or digital Polymerase Chain Reaction (PCR) instruments; medical diagnostic assays for testing of body samples, including bodily fluids and tissue in the detection, monitoring, and prediction of disease or other conditions, disease states, hormone level, drug level, drug response, organ function effectiveness, and clinical response; diagnostic test kits comprised of medical diagnostic reagents and assays for testing in the field of medical diagnostics. Apparatus for medical and veterinary diagnostic analysis and determination of medical and veterinary conditions, namely DNA and RNA testing apparatus; Medical and veterinary equipment for diagnostic purposes; clinical medical laboratory apparatus and analytical instruments, namely digital Polymerase Chain Reaction (d PCR) instruments for use in in-vitro and in-vivo diagnostics; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (dPCR) platforms; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (dPCR) platform; medical devices, namely, digital polymerase chain reaction (dPCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (dPCR) platform; microfluidic plates for use as medical apparatus. Testing services, namely, quantifying DNA and RNA for medical, diagnostic and treatment purposes; rental of digital Polymerase Chain Reaction (PCR) apparatus; RNA or DNA analysis for cancer diagnosis and prognosis; Medical services, in particular medical analysis in connection with the treatment of individuals; providing information in the fields of medicine, veterinary medicine and pharmaceuticals; health centers, namely, genetic centers providing medical diagnostic, preventive and curative therapy services for genetic disorders, cancer treatment centers, rare disease treatment centers, children's hospitals and women's hospital; medical clinics; gene therapy services in the field of oncology, infertility, prenatal diagnostics, carrier testing, and genetically inherited diseases; medical laboratory services for the analysis of samples taken from patients.
The present invention provides methods and kits for virus inactivation by virus-deactivating substances and direct amplification-based detection of target nucleic acids and viruses in biological samples without prior target purification.
The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.
The present invention concerns a method for isolating DNA molecules having a size above a certain cut-off value from a DNA-containing sample. The method comprises a) contacting the sample with an aqueous composition comprising beads with a negatively charged surface, a molecular crowding agent, a dissolved salt comprising at least one divalent metal cation, and optionally a buffer for a time sufficient to bind DNA to the surface of the beads; b) separating the beads with bound DNA from the remaining composition; c) optionally washing the beads; and d) optionally eluting the bound DNA from the beads; wherein the ratio of the concentration of salt to the concentration of beads in the aqueous composition in step a) is at a value where an increase in the ratio leads to an increase in said cut-off value.
The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
A phenol-free method for isolating a nucleic acid from a sample is provided, said method comprising the following steps: a) adding a precipitation buffer to a sample to prepare an acidic precipitation mixture wherein said precipitation buffer comprises a metal cation precipitant and a buffering agent, has a pH value of 4.0 or less and does not comprise an organic solvent selected from aprotic polar solvents and protic solvents and wherein the acidic precipitation mixture comprises the metal cation precipitant in a concentration of less than 200 mM and precipitating proteins; b) separating the precipitate from the supernatant, wherein the supernatant comprises small RNA having a length of less than 200 nt and large RNA having a length of at least 1000 nt; and c) isolating a nucleic acid from the supernatant. The present method allows to avoid the use of organic solvents during protein precipitation. Also provided is a precipitation buffer.
The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.
The present invention relates to a method of classifying a patient that suffers from or is at risk of developing urothelial or bladder cancer, said method comprising the steps of taking a liquid sample from the patient, extracting RNA from said liquid sample, and determining in said RNA, the expression level of at least one gene encoding for a receptor selected from the group consisting of FGFR2, FGFR3 or ErbB2, and classifying the sample of said patient into one of at least two classifications (Fig. 2).
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
The present invention relates to a method for amplifying a target RNA in a sample, an oligonucleotide usable in the method according to the invention, a kit for amplifying a target RNA, and a use of an oligonucleotide for inhibiting an aptamer oligonucleotide.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
52.
SIGNAL NORMALIZATION OF NUCLEIC ACID AMPLIFICATION REACTION PRODUCTS
The invention relates to a multiplex nucleic acid amplification reaction comprising at least two separate amplicons, with normalized signal strength, wherein for at least one amplicon either the forward primer oligonucleotides, or the reverse primers oligonucleotides, or the optional detection probe oligonucleotides, are only partially labeled, and the subset amount of labeled primer/probe oligonucleotides to subset amount of unlabeled primer/probe oligonucleotides is adjusted so that the signal strength in both amplicons is equal or nearly equal. Kits and compositions are also claimed.
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
53.
NUCLEIC ACID PURIFICATION FROM FIXED BIOLOGICAL SAMPLES
The present invention provides a method for lysing a fixed biological sample, wherein the fixed biological sample comprises crosslinks between nucleic acid molecules and protein molecules due to the fixation, said method comprising (a) lysing the fixed biological sample, wherein lysis involves digestion with a proteolytic enzyme; (b) heating the lysed sample to reverse crosslinks; (c) adding a proteolytic enzyme and performing a proteolytic digestion; optionally wherein one or more additional treatment steps are performed between step (b) and step (c). The provided nucleic acids are of high yield and quality and can be purified from the lysed sample.
The invention relates to the use of a compound, wherein the compound is a fluorescence dye with a high Stokes shift, which is large enough to skip at least one intermediate emission filter channel, i.e., to jump at least into the next but one channel, as reference dye for non-droplet based dPCR systems to evaluate filling levels of compartments, as reference dye for fluorescence PCR systems for normalization or as dye for target detection for multiplex PCR, wherein the compound is bound to a probe or primer as fluorescent label, wherein the PCR is selected from the group consisting of digital PCR (dPCR), quantitative PCR (qPCR), reverse transcription PCR (RT-PCR), reverse transcription quantitative PCR (RT-qPCR) and real-time PCR.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Single-use cartridges for clinical laboratory use;
single-use cartridges for clinical laboratory use containing
nucleic acid extraction column and/or real time Polymerase
Chain Reaction chambers. Medical diagnostic apparatus and instruments; analysers for
medical use; analysers for medical diagnosis; medical and
clinical diagnosis apparatus and machines, namely molecular
and immunoassay analyzers for analyzing blood and bodily
fluids; testing instruments for medical diagnostic purposes;
apparatus for carrying-out diagnostic tests for medical
purposes.
56.
METHOD OF EXAMINING A NUCLEIC ACID AMPLIFICATION PRODUCT
The invention relates to a method for examining a nucleic acid amplification product, comprising the steps of a. providing an amplification product stemming from a preferably exponentialamplification comprising i. an upstream and downstream primer region as well as optionally and preferably, ii. an amplified target region between said upstream and downstream primer region,b. examining the amplification product by amplifying said amplification product in a PCR reaction, wherein i. the upstream and downstream PCR primers in said PCR reaction bind in the upstream and downstream primer region of the amplification product, ii. a first and a second oligonucleotide probe are placed downstream of the first and second PCR primers and within the upstream and the downstream primer region, c. performing a PCR reaction and examining the newly formed amplification product, wherein the upstream and downstream primer regions of step a.i.) have a length of under 35 nucleotides and over 20 nucleotides.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Single-use cartridges for clinical laboratory use; single-use cartridges for clinical laboratory use containing nucleic acid extraction column and/or real time Polymerase Chain Reaction chambers Medical diagnostic apparatus and instruments for detecting tumors, cancer and genetic diseases, for DNA and RNA testing for medical purposes, for diagnostic testing of body fluid, body tissue and organ samples, for diagnostic medical testing for cancer, for detecting and treating tumors and cancer, for detecting genetic predispositions, for detecting pathogens and disease biomarkers; medical apparatus and instruments for diagnostic use, namely, apparatus for medical diagnostic testing in the fields of cancer or other tissue-based diagnostic testing, cytology and cell-based testing; analysers for medical use for detecting tumors, cancer and genetic diseases; medical diagnostic apparatus and instruments, namely, DNA sequencers; analysers for medical diagnosis of tumors, cancer, genetic diseases, genetic predispositions, pathogens and disease biomarkers; testing instruments for medical diagnostic purposes for detecting tumors, cancer, genetic diseases, genetic predispositions, pathogens and disease biomarkers; apparatus for carrying-out diagnostic tests for medical purposes for detecting tumors, cancer, genetic diseases, genetic predispositions, pathogens and disease biomarkers; medical and clinical diagnosis apparatus and machines, namely, molecular and immunoassay analyzers for analyzing blood and bodily fluids
A method is provided for enriching extracellular DNA from a biological sample comprising extracellular DNA and extracellular vesicles, wherein the method comprises: (a) preparing a binding mixture comprising—the biological sample, —a solid phase comprising anion exchange groups, —an acidic binding buffer comprising a buffering agent, and binding extracellular DNA to the solid phase comprising anion exchange groups; (b) separating the solid phase with the bound extracellular DNA from the remaining binding mixture, wherein the remaining binding mixture comprises extracellular vesicles. The method may furthermore comprise processing the remaining binding mixture to enrich one or more biological targets of interest therefrom, wherein processing may comprise (c) enriching as biological targets extracellular vesicles and/or extracellular RNA from the remaining binding mixture.
The present disclosure relates to a method of evaluating a mutational burden in a sample. The method includes providing first data which represents allelic depths of the sample; calculating rate data based on the first data; segmenting the rate data into a plurality of segments of rate data using a wavelet transform; estimating parameters for each of the plurality of segments; classifying each of the plurality of segments based on the estimated parameters. The classifying includes, for each of a plurality of classification thresholds, generating a classification for the plurality of segments based on the classification threshold and determining an intermediate value based on the classification. The method further comprises determining a mutational burden of the sample based on the intermediate values.
The present invention pertains to methods and kits for enriching extracellular nucleic acids such as vesicular RNA from a sample comprising extracellular vesicles. Accordingly to the methods an acidic binding mixture is prepared comprising the sample and anion exchange particles and binding extracellular vesicles to the anion exchange particles. After separating the anion exchange particles comprising the bound extracellular vesicles from the remaining mixture, bound extracellular vesicles are lysing the in the presence of at least one detergent and released RNA is bound to the anion exchange particles. The anion exchange particles with the bound RNA from the lysate are then eluted.
The present invention provides methods and kits for isolating cell-free, non-vesicular miRNAs from a biological sample, such as serum and plasma, by using an acidic binding buffer comprising a buffering agent to promote binding of non-vesicular miRNAs to a solid phase comprising anion exchange groups. Furthermore, binding of non-vesicular miRNAs to the solid phase may be improved due to the presence of a crowding agent and by increasing the surface charge density of the solid phase.
The present invention provides methods for visualization, reviewing and manipulating of dPCR data. Furthermore, the present invention provides systems for dPCR data visualization, reviewing and manipulating and computer-readable storage media with instructions for dPCR data visualization, reviewing and manipulating. In addition the use of such systems and computer-readable storage media for dPCR data visualization, reviewing and manipulating is provided.
The present invention provides methods for visualization, reviewing and manipulating of dPCR data. Furthermore, the present invention provides systems for dPCR data visualization, reviewing and manipulating and computer-readable storage media with instructions for dPCR data visualization, reviewing and manipulating. In addition the use of such systems and computer-readable storage media for dPCR data visualization, reviewing and manipulating is provided.
The present invention provides methods and composition suitable for stabilizing cell-containing samples such as blood samples. The stabilizers used are primary or secondary carboxylic acid amides.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Single-use cartridges for clinical laboratory use; Single-use cartridges for clinical laboratory use containing nucleic acid extraction column and/or real time Polymerase Chain Reaction chambers. Medical diagnostic apparatus and instruments; Analysers for medical use; Analysers for medical diagnosis; Medical and clinical diagnosis apparatus and machines, namely molecular and immunoassay analyzers for analyzing blood and bodily fluids; Testing instruments for medical diagnostic purposes; Apparatus for carrying-out diagnostic tests for medical purposes.
67.
AUTOMATED METHOD AND SYSTEM FOR SPLIT POOL BASED BARCODING OF CELLULAR MOLECULES
C12Q 1/6811 - Méthodes de sélection pour la production ou l’élaboration d’oligonucléotides spécifiques cibles ou de molécules de liaison
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
68.
MULTIMODAL ANALYSIS OF STABILIZED CELL-CONTAINING BODILY FLUID SAMPLES
A method for stabilizing and isolating multiple biological targets comprised in a cell-containing bodily fluid, said method comprising (A) contacting a cell-containing bodily fluid with a stabilizing composition comprising one or more of the following stabilizing agents: (a) at least one primary, secondary or tertiary amide, (b) at least one poly(oxyethylene) polymer, and/or (c) at least one apoptosis inhibitor, thereby providing a stabilized cell-containing bodily fluid sample; (B) keeping the stabilized cell-containing bodily fluid sample for a stabilization period; and (C) processing the stabilized cell-containing bodily fluid sample in order to enrich three or more biological targets selected from the group consisting of —a cell subpopulation, —extracellular nucleic acids, —extracellular vesicles and —intracellular nucleic acids from the stabilized cell-containing bodily fluid. The method is advantageous and enables the multimodal analyses of different biological targets from a single stabilized cell-containing body fluid sample.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
The present invention provides a poly(alkylene oxide) polymer based size selective nucleic acid enrichment method for enriching target nucleic acid molecules from a nucleic acid containing sample which comprises target nucleic acid molecules and non-target nucleic acid molecules, wherein the target nucleic acid molecules are longer than the non-target nucleic acid molecules, the method comprising (a) preparing a binding mixture comprising—the nucleic acid containing sample, —a poly(alkylene oxide) polymer and—a salt and binding nucleic acid molecules to a solid phase which comprises a functional group, preferably carboxylated magnetic particles, wherein the bound nucleic acid molecules comprise target nucleic acid molecules; (b) preferably separating the solid phase with the bound nucleic acid molecules from the remaining sample; (c) contacting the solid phase with the bound nucleic acid molecules at least once with a reagent composition comprising a poly(alkylene oxide) polymer and a salt to selectively elute non-target nucleic acid molecules, wherein the concentration (w/v) of the poly(alkylene oxide) polymer in the reagent composition of step (c) is lower than the concentration (w/v) of the poly(alkylene oxide) polymer in the binding mixture of step (a); (d) optionally washing the bound target nucleic acid molecules; and (e) eluting the bound target nucleic acid molecules from the solid phase. Said method allows the size selective purification of target DNA molecules and is particularly suitable for sequencing applications. It is more time- and cost efficient than prior art methods and provides excellent purification results. Moreover, further methods and kits are provided.
An oligonucleotide suitable for use as a detection probe in a method for evaluating the amplification efficiency, the presence of inhibitors, degradation and/or for performing a quantification analysis of a target nucleic acid in a real-time amplification reaction. The oligonucleotide probe is preferably SEQ ID NO. 4, a reverse complement of SEQ ID NO. 4, an oligonucleotide that shares 95% sequence identity with SEQ ID NO. 4, or an oligonucleotide that shares 95% sequence identity with a reverse complement of SEQ ID NO. 4. Also a kit including an internal nucleic acid control template, a set of primers for amplifying the internal nucleic acid control template, and the oligonucleotide probe.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
The present invention provides methods and kits for virus inactivation by virus-deactivating substances and direct amplification-based detection of target nucleic acids and viruses in biological samples without prior target purification.
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
The present invention provides a poly(alkylene oxide) polymer based size selective method for enriching nucleic acid molecules having a length below a cut-off value from a nucleic acid containing sample, the method comprising: (a) preparing a binding mixture comprising —the nucleic acid containing sample, —a poly(alkylene oxide) polymer and —a salt and binding nucleic acid molecules of different sizes to a solid phase which comprises a functional group, preferably carboxylated magnetic particles; (b) separating the solid phase with the bound nucleic acid molecules from the remaining sample; and (c) contacting the solid phase with the bound nucleic acid molecules at least once with an elution composition comprising a poly(alkylene oxide) polymer and a salt to selectively elute nucleic acid molecules having a length below the cut-off value from the solid phase while larger nucleic acid molecules having a length above the cut-off value remain bound to the solid phase, wherein the concentration (w/v) of the poly(alkylene oxide) polymer in the elution composition is lower than the concentration (w/v) of the poly(alkylene oxide) polymer in the binding mixture of (a); (d) separating the solid phase with the bound larger nucleic acid molecules from the eluted nucleic acid molecules; and (e) optionally further purifying the eluted nucleic acid molecules. The method is particularly useful for separating extracellular target nucleic acids by size.
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Retail store services featuring virtual goods, namely, chemical reagents, laboratory robots, heaters, shakers, pipetting instruments, and embedded operating software on the foregoing, software to review or analyze scientific, diagnostic, and laboratory data, and diagnostic reagents, for use in on-line virtual worlds; On-line virtual retail store services rendered in a virtual environment featuring physical goods and virtual goods, namely, chemical reagents, laboratory robots, heaters, shakers, pipetting instruments, and embedded operating software on the foregoing, software to review or analyze scientific, diagnostic, and laboratory data, and diagnostic reagents; organizing and conducting virtual reality and augmented reality events, exhibitions, expositions and conferences for commercial purposes in the fields of biotechnology, life sciences and diagnostics; providing incentive and loyalty award programs for customers through the issuance of tokens for purchase of a company's goods and services for promoting the goods and services of others Entertainment and educational services, namely, providing meetings, conferences, webinars, in virtual reality and augmented reality environments for education of scientists and consumers in the use of chemical reagents, laboratory robots, heaters, shakers, pipetting instruments, and embedded software on the foregoing, software to review or analyze scientific, diagnostic, and laboratory data, and diagnostic reagents; providing meetings, conferences, webinars in virtual reality and augmented reality environments for education in the field of marketing and promotion of chemical reagents, laboratory robots, heaters, shakers, pipetting instruments, and embedded software on the foregoing, software to review or analyze scientific, diagnostic, and laboratory data, and diagnostic reagents; counseling services in the fields of biotechnology, life sciences and diagnostics for scientists and consumers, namely, training in the use of chemical reagents, laboratory robots, heaters, shakers, pipetting instruments, and embedded software on the foregoing, software to review or analyze scientific, diagnostic, and laboratory data, and diagnostic reagents, and scientific data interpretation Providing reagent sample testing and diagnostic services for others for scientific research purposes provided in virtual reality and augmented reality environments Providing information, news and commentary in the field of life sciences in virtual reality and augmented reality environments; Providing information, news and commentary in the field of medical diagnostic tests; medical diagnostic testing services provided in virtual reality and augmented reality environments; consulting services in the fields of diagnostic medical testing provided in virtual reality and augmented reality environments; providing healthy lifestyle services, namely, personal assessments, personalized routines, maintenance schedules, and counseling; providing healthcare information to others in virtual reality and augmented reality environments
The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.
The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.
The present invention provides methods and kits for virus inactivation by virus-deactivating substances and direct amplification-based detection of target nucleic acids and viruses in biological samples without prior target purification.
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
The present disclosure provides a method for stabilizing a urine sample comprising contacting the urine sample for stabilization with EDTA, at least one poly(oxyethylene) polymer and a preservative for inhibiting bacterial growth. Also provided are stabilizing compositions and methods for processing stabilized urine sample.
A kit for use in assessing the status of nucleic acid degradation and/or the integrity of one or more nucleic acids in a sample by amplifying at least two overlapping regions within at least one locus and detecting the amount of the at least two amplification products. The kit includes a primer and at least two probes that bind under stringent conditions to a sequence that shares at least 80% sequence identity to a sequence selected from the group of sequences consisting of SEQ ID NO. 6 to SEQ ID NO. 47 over a stretch of 80 base pairs, or to a reverse complement thereof. One of the at least two probes binds to one of the at least two overlapping regions and the other of the at least two probes binds to a non-overlapping region.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using an apoptosis inhibitor, preferably a caspase inhibitor, a hypertonic agent and/or a compound according to formula 1 as defined in the claims.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
83.
Method for isolating extracellular nucleic acids using anion exchange particles
The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for
industrial and scientific purposes in particular nucleic
acids, enzymes, polymerases, reagents for staining or
marking, buffers, aqueous and non-aqueous solutions of
alcohols and/or salts, distilled or purified water, silica,
magnetic particles; chemical, biochemical and
biotechnological products for industrial and scientific
purposes in particular materials for chromatography columns;
reagents and/or solvents for sample preparation,
purification, lysis, modification and/or manipulation of
cells, as well as for a process of marking, separation,
isolation, purification, amplification and/or analysis of
biopolymers, in particular nucleic acids, enzymes, proteins,
macro molecules and biologically active substances; kits
comprising chemical, biochemical and biotechnological
products for sample preparation, purification, lysis,
modification and/or manipulation of cells as well as for a
process of marking, separation, isolation, purification,
amplification and/or analysis of biopolymers, in particular
nucleic acids, enzymes, proteins, macro molecules and
biologically active substances, in particular nucleic acids
from biological and biochemical sample material. Diagnostics for medical purposes, products for medical and
veterinary medical purposes; diagnostic preparations, in
particular nucleic acids, enzymes, polymerases, reagents for
staining or marking, buffers, aqueous and non-aqueous
solutions of alcohols and/or salts, distilled or purified
water, silica, magnetic particles, materials; diagnostics
for medical purposes, products for medical and veterinary
medical purposes, in particular materials for chromatography
columns; chemical, biochemical and biotechnological products
as well as kits comprising essentially products for medical
and veterinary medical purposes, in particular chemical,
biochemical and biotechnological products for sample
preparation, purification, lysis, modification and/or
manipulation of cells; biochemical and biotechnological
products for medical and veterinary medical purposes, in
particular for a process of marking, separation, isolation,
purification, amplification and/or analysis of biopolymers,
in particular nucleic acids, enzymes, proteins, macro
molecules and biologically active substances. Scientific apparatus, instruments and equipment, in
particular for research, development and examination
purposes; scientific apparatus, instruments and equipment,
in particular laboratory equipment, analytical instruments,
diagnostic instruments not for medical use on which products
for medical and veterinary medical purposes may be used;
scientific apparatus, instruments and laboratory equipment,
analytical instruments, diagnostic instruments not for
medical use in relation to sample preparation, modification
and manipulation of cells; scientific apparatus, instruments
and laboratory equipment, analytical instruments, diagnostic
instruments not for medical use in particular for a process
of marking, separation, isolation, purification,
amplification and/or analysis of biopolymers, in particular
nucleic acids, proteins, macro molecules and biologically
active substances, in particular nucleic acids from
biological and biochemical sample material; chromatography
columns, automated laboratory equipment and spare parts and
add-ons, in particular pre-programmed cards, micro plates,
pipettes, pipette tips, pipette tip holders, sample
containers, reagent holders, flasks, containers, reagent
tube holders, holders for micro containers, magnetic
particles, magnetic syringes, biochips, thermocycler,
homogenizers, vacuum valves.
The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Chemical, biochemical and biotechnological diagnostic
preparations for scientific use, non-medical reagents used
in analytical chemistry, for scientific and/or medical
research use for marking, separating, isolating, amplifying,
cloning, sequencing and/or for the analysis of biopolymers,
in particular nucleic acids, in particular reagents, enzymes
and buffer solutions for isolating, amplifying and analysing
nucleic acids; chemical test kits for laboratory or research
use for marking, separating, isolating, amplifying, cloning,
sequencing and/or for the analysis of biopolymers, in
particular nucleic acids, in particular comprising reagents,
enzymes and buffer solutions for isolating, amplifying and
analysing nucleic acids. Diagnostic preparations for medical and veterinary purposes,
namely diagnostic preparations for marking, separating,
isolating, amplifying, cloning, sequencing and/or for the
analysis of biopolymers, namely nucleic acids; diagnostic
reagents, enzymes and buffer solutions for isolating,
amplifying and analysing nucleic acids for medical or
veterinary purposes; chemical, biochemical and
biotechnological diagnostic preparations for medical and
veterinary purposes, namely reagents, enzymes and buffer
solutions for marking, separating, isolating, amplifying,
cloning, sequencing and/or for the analysis of biopolymers,
namely nucleic acids; diagnostic kits containing
preparations for medical and veterinary diagnostic purposes,
namely preparations for marking, separating, isolating,
amplifying, cloning, sequencing and/or for the analysis of
biopolymers, namely nucleic acids; diagnostic kits
containing reagents, enzymes and buffer solutions for
isolating, amplifying and analysing nucleic acids for
medical or veterinary purposes.
The present invention relates to a method for the treatment of a biological material, comprising the steps
i) providing a biological material, and
ii) contacting the biological material with a first non-aqueous composition comprising:
(a1) 10 to 90 vol. % methanol, and
(a2) at least one additional additive, and
(a3) optionally an acid.
iii) transferring the biological material into a second composition (B) comprising up to 99 vol. % ethanol
as well as to a new composition for preservation of a biological material usable in said method and the biological material resulting from this method, a method for the analysis of a treated biological material, various kits as well as the use of the composition in such a method.
Method and system for compensating intensity biases in a plurality of digital images. Each digital image of the plurality of digital images contains a plurality of objects and each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive one of at least a first fluorescent compound and a second fluorescent compound. A first digital image of the plurality of digital images is taken by an optical imaging system during emission of electromagnetic radiation by the first fluorescent compound, and a second digital image of the plurality of digital images is taken by the optical imaging system during emission of electromagnetic radiation by the second fluorescent compound.
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 40/10 - Traitement du signal, p. ex. de spectrométrie de masse ou de réaction en chaîne par polymérase
The present invention pertains inter alia to a method for isolating poly(A) nucleic acids having a single stranded poly(A) stretch from a nucleic acid containing sample comprising: (a) providing a hybridization composition comprising: i) a nucleic acid containing sample; ii) a hybridization solution comprising: aa. a sodium salt; bb. a quaternary ammonium salt; wherein the components of the hybridization solution can be added as single solution to the sample or may be added separately in any order to the sample; iii) a capture probe capable of hybridizing to the poly(A) stretch of the poly(A) nucleic acids; and incubating said hybridization composition under conditions to form nucleic acid-hybrids between the poly(A) nucleic acids and the capture probe; (b) separating the formed hybrids from the remaining sample. The method is in particular suitable for efficiently isolating poly(A) RNA from various samples while avoiding carry-over of unwanted non-poly(A) nucleic acids such as rRNA. Also provided are advantageous further methods, hybridization solutions and kits.
The present invention relates to a method for the treatment of a biological material, comprising the steps
i) providing a biological material, and
ii) contacting the biological material with a first non-aqueous composition comprising:
(a1) 10 to 90 vol. % methanol, and
(a2) at least one additional additive, and
(a3) optionally an acid.
iii) transferring the biological material into a second composition (B) comprising up to 99 vol. % ethanol
as well as to a new composition for preservation of a biological material usable in said method and the biological material resulting from this method, a method for the analysis of a treated biological material, various kits as well as the use of the composition in such a method.
The invention relates to a method of neighbor influence compensation between a plurality of objects in at least one digital image, wherein the at least one digital image contains image information about a plurality of objects. Each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive a fluorescent compound, and the at least one digital image is taken by an optical imaging system during emission of electromagnetic radiation of the fluorescent compounds received by the at least one molecules.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Chemical, biochemical and biotechnological diagnostic
preparations for scientific use, non-medical reagents used
in analytical chemistry, for scientific and/or medical
research use for marking, separating, isolating, amplifying,
cloning, sequencing and/or for the analysis of biopolymers,
in particular nucleic acids, in particular reagents, enzymes
and buffer solutions for isolating, amplifying and analysing
nucleic acids; chemical test kits for laboratory or research
use for marking, separating, isolating, amplifying, cloning,
sequencing and/or for the analysis of biopolymers, in
particular nucleic acids, in particular comprising reagents,
enzymes and buffer solutions for isolating, amplifying and
analysing nucleic acids; diagnostic kits containing
reagents, enzymes and buffer solutions for isolating,
amplifying and analysing nucleic acids for medical or
veterinary purposes. Diagnostic preparations for medical and veterinary purposes,
namely diagnostic preparations for marking, separating,
isolating, amplifying, cloning, sequencing and/or for the
analysis of biopolymers, namely nucleic acids; diagnostic
reagents, enzymes and buffer solutions for isolating,
amplifying and analysing nucleic acids for medical or
veterinary purposes; chemical, biochemical and
biotechnological diagnostic preparations for medical and
veterinary purposes, namely reagents, enzymes and buffer
solutions for marking, separating, isolating, amplifying,
cloning, sequencing and/or for the analysis of biopolymers,
namely nucleic acids; diagnostic kits containing
preparations for medical and veterinary diagnostic purposes,
namely preparations for marking, separating, isolating,
amplifying, cloning, sequencing and/or for the analysis of
biopolymers, namely nucleic acids; diagnostic kits
containing reagents, enzymes and buffer solutions for
isolating, amplifying and analysing nucleic acids for
medical or veterinary purposes.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Nucleic acids, enzymes, polymerases, reagents for staining and marking, buffers, aqueous and non aqueous solutions of alcohols and salts, distilled and purified water, silica in powdered, granulate and gel form and magnetic particles in liquid form for analysis of biological and biochemical samples for scientific and research laboratory use; chemical, biochemical and biotechnological products for industrial and scientific purposes in particular materials for chromatography columns; reagents and solvents for sample preparation, purification, lysis, modification and manipulation of cells, as well as for marking, separation, isolation, purification, amplification and analysis of biopolymers, namely, nucleic acids, enzymes, proteins, macro molecules and biologically active substances, namely, nucleic acids from biological and biochemical sample material; reagent kits comprising chemical, biochemical and biotechnological products, other than for medical or veterinary use, for sample preparation, purification, lysis, modification and/or manipulation of cells as well as for marking, separation, isolation, purification, amplification and analysis of biopolymers, namely, nucleic acids, enzymes, proteins, macro molecules and biologically active substances, namely, nucleic acids from biological and biochemical sample material
(2) Diagnostic agents and preparations for medical diagnostic use and veterinary diagnostic use, namely, nucleic acids, enzymes, polymerases, reagents for staining and marking, buffers, aqueous and non-aqueous solutions of alcohols and salts, distilled and purified water, silica in powdered, granulate and gel form and, magnetic particles in liquid form; diagnostics agents and preparations for medical purposes, products for medical and veterinary medical purposes, in particular materials for chromatography columns; reagent kits for medical and veterinary purposes comprised of diagnostic preparations for medical and veterinary medical diagnostic purposes for the preparation of samples, the modification and manipulation of samples and for marking, separation, isolation, purification, reproduction and analysis of biopolymers, namely, nucleic acids, enzymes, proteins, macromolecules and biologically active substances in the nature of nucleic acids from biological and biochemical sample materials; reagent kits comprising essential diagnostic reagents and assays for medical and veterinary diagnostic purposes, namely, chemical, biochemical and biotechnological preparations for sample preparation, purification, lysis, modification and manipulation of cells; biochemical and biotechnological agents and preparations for medical diagnostic use and veterinary diagnostic use for marking, separation, isolation, purification, amplification and/or analysis of biopolymers, namely, nucleic acids, enzymes, proteins, macro molecules and biologically active substances
(3) Laboratory instruments for the analysis and isolation of nucleic acids and proteins in a biological sample for research use; scientific apparatus, instruments and equipment, namely, thermocyclers and automated sample-processing platforms not for medical use for marking, separation, isolation, purification, amplification and analysis of biopolymers, namely nucleic acids, proteins, macro molecules and biologically active substances, namely, nucleic acids from biological and biochemical sample material; chromatography columns; automated laboratory equipment, namely, magnetic particles, thermoyclers for the purpose of performing polymerase chain reactions, homogenizers to homogenize samples for subsequent isolation of nucleic acids and proteins, vacuum valves for the purpose of permitting the operation of laboratory processes under subatmospheric pressure; sample preparation instruments for isolation of nucleic acid or proteins and spare parts and add-ons, namely, pre-programmed cards, micro plates, pipettes, pipette tips, pipette tip holders, sample containers, reagent holders, flasks, reagent cartridges, reagent tube holders, holders for micro containers, magnetic particles, magnetic syringes, biochips, thermocycler, homogenizers, vacuum valves, for laboratory use
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for industrial and scientific purposes, namely, nucleic acids, enzymes, polymerases, reagents for staining or marking, buffers, aqueous and non-aqueous solutions of alcohols and salts, distilled or purified water, silica-coated particles in liquid form; magnetic particles in liquid form; reagents and solvents for sample preparation, purification, lysis, modification and manipulation of cells, as well as for marking, separation, isolation, purification, amplification and analysis of biopolymers, in particular nucleic acids, enzymes, proteins, macro molecules and biologically active substances, in particular nucleic acids from biological and biochemical sample material; reagent kits comprising chemical, biochemical and biotechnological products, other than for medical or veterinary use, for sample preparation, purification, lysis, modification and manipulation of cells, as well as for marking, separation, isolation, purification, amplification and analysis of biopolymers, namely, nucleic acids, enzymes, proteins, macro molecules and biologically active materials Diagnostic agents and preparations for medical purposes; diagnostic preparations for medical and veterinary medical purposes, namely, nucleic acids, enzymes, polymerases, reagents for staining or marking, buffers, aqueous and non-aqueous solutions of alcohols and salts, distilled or purified water, silica and magnetic particles; kits comprising diagnostic preparations for medical and veterinary medical diagnostic purposes for the preparation of samples, the modification and manipulation of samples and for marking, separation, isolation, purification, reproduction and analysis of biopolymers, namely, nucleic acids, enzymes, proteins, macromolecules and biologically active substances in the nature of nucleic acids from biological or biochemical sample materials; reagent kits comprising essential diagnostic reagents and assays for medical and veterinary diagnostic purposes, namely, chemical, biochemical and biotechnological preparations for sample preparation, purification, lysis, modification and manipulation of cells; diagnostic preparations for medical and veterinary purposes for marking, separation, isolation, purification, amplification and analysis of biopolymers, namely, enzymes, proteins, macromolecules and biologically active substances in the nature of nucleic acids from biochemical and biotechnological sample materials Scientific apparatus, instruments and equipment, namely, sample preparation devices for isolation of nucleic acids and proteins for research, development and examination purposes; scientific apparatus, instruments and laboratory equipment, namely, thermocyclers and automated sample-processing platforms, not for medical use, for marking, separation, isolation, purification, amplification and analysis of biopolymers, in particular nucleic acids, proteins, macro molecules and biologically active substances, in particular nucleic acids from biological and biochemical sample material; chromatography columns for laboratory use; sample preparation instruments for isolation of nucleic acid or proteins and spare parts and add-ons, namely, laboratory pipettes, laboratory pipette tips, laboratory pipette tip holders; laboratory equipment, namely, magnetic particles, thermocyclers for the purpose of performing polymerase chain reactions, homogenizers to homogenize samples for subsequent isolation of nucleic acids or proteins, vacuum valves for the purpose of permitting the operation of laboratory processes under subatmospheric pressure
95.
Nucleic acid purification from fixed biological samples
The present invention provides a method for lysing a fixed biological sample, wherein the fixed biological sample comprises crosslinks between nucleic acid molecules and protein molecules due to the fixation, said method comprising (a) lysing the fixed biological sample, wherein lysis involves digestion with a proteolytic enzyme; (b) heating the lysed sample to reverse crosslinks; (c) adding a proteolytic enzyme and performing a proteolytic digestion; optionally wherein one or more additional treatment steps are performed between step (b) and step (c). The provided nucleic acids are of high yield and quality and can be purified from the lysed sample.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical, biochemical and biotechnological products for use
in industry or research use; chemical, biochemical and
biotechnological products for non-medical purposes;
chemical, biochemical and biotechnological products for use
in in-vitro diagnostic for non-medical purposes; chemical,
biochemical and biotechnological products for use in
scientific and laboratory research; chemical, biochemical
and biotechnological products for use in in-vitro diagnostic
for scientific research or laboratory use; reagent kits
comprising chemical, biochemical and biotechnological
products for industrial and scientific purposes; reagent
kits comprising chemical, biochemical and biotechnological
products for use in in-vitro diagnostic for non-medical
purposes; chemical, biochemical and biotechnological test
kits for in-vitro detection of pathogens, viruses and
bacteria for laboratory or research use; biochemicals,
namely antibodies for in-vitro scientific or research use;
chemical, biochemical and biotechnological kits comprising
antibodies for in-vitro detection of pathogens, viruses and
bacteria. Chemical, biochemical and biotechnological products for
medical and veterinary purposes; chemical, biochemical and
biotechnological products for human in-vitro diagnostic
purposes; chemical, biochemical and biotechnological
reagents and preparations for human in-vitro diagnostic
purposes; kits comprising chemical, biochemical and
biotechnological products for human in-vitro diagnostic;
chemical, biochemical and biotechnological products for
medical and pharmaceutical research; diagnostic kits
comprising chemical products for human in-vitro diagnostic
for medical or pharmaceutical purposes; diagnostic kits
comprising chemical, biochemical and biotechnological
products for human in-vitro diagnostic for detection of
pathogens, viruses and bacteria for medical or
pharmaceutical purposes; biochemicals, namely antibodies for
use in human in-vitro medical diagnostic; chemical,
biochemical and biotechnological kits for human in-vitro
diagnostic comprising antibodies for in-vitro detection of
pathogens, viruses and bacteria. Scientific apparatuses, instruments and devices, as well as
their parts and components included in this class; software
for use in scientific and medical research; scientific
apparatuses, instruments and devices; software in the area
of detection of pathogens, viruses and bacteria, in
particular detection by specific antibodies.
97.
METHOD FOR ISOLATING NUCLEIC ACIDS FROM PLANT SAMPLES
Provided is a method for isolating nucleic acids from a plant sample comprising (a) preparing a lysed sample wherein preparing comprises (i) lysing a plant sample by mechanically disrupting the plant sample in a lysis solution which comprises at least one chaotropic agent and one or more solid disrupting particles, and (ii) optionally clearing the lysate; (b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture; (c) obtaining a liquid phase from the mixture; and (d) isolating nucleic acids from the liquid phase. Also provided is a kit for use in such method.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Chemical, biochemical and biotechnological diagnostic
preparations used in science; non-medical reagents, enzymes
and chemicals, used in analytical chemistry, for scientific
and/or medical research use for marking, separating,
isolating, amplifying, cloning, sequencing and/or for the
analysis of biopolymers, in particular nucleic acids, in
particular reagents, enzymes and buffer solutions for
nucleic acid isolation, amplification and analysis; chemical
test kits for laboratory or research use for marking,
separating, isolating, amplifying, cloning, sequencing
and/or for the analysis of biopolymers, in particular
nucleic acids, in particular comprising reagents, enzymes
and buffer solutions for nucleic acid isolation,
amplification and analysis. Diagnostic preparations for medical and veterinary purposes,
namely diagnostics preparations for marking, separating,
isolating, amplifying, cloning, sequencing and/or for the
analysis of biopolymers, namely nucleic acids; diagnostic
reagents, enzymes and buffer solutions for nucleic acid
isolation, amplification and analysis for medical or
veterinary purposes; chemical, biochemical and
biotechnological diagnostic preparations for medical and
veterinary purposes, namely reagents, enzymes and buffer
solutions for marking, separating, isolating, amplifying,
cloning, sequencing and/or for the analysis of biopolymers,
namely nucleic acids; diagnostic kits containing
preparations for medical and veterinary diagnostic purposes,
namely for marking, separating, isolating, amplifying,
cloning, sequencing and/or for the analysis of biopolymers,
namely nucleic acids; diagnostic kits containing reagents,
enzymes and buffer solutions for nucleic acid isolation,
amplification and analysis for medical or veterinary
purposes.
The present invention provides a lysis method for releasing microbial nucleic acids from microorganisms comprised in a plant sample, comprising mechanically disrupting the plant sample in a liquid lysis composition using at least two types of solid disrupting particles, wherein (i) the first type is provided by one or more disrupting particles having a size of at least 1.5 mm and (ii) the second type is provided by a plurality of disrupting particles having a size of 1 mm or less. Also provides is a method for isolating nucleic acids including microbial nucleic acids from a plant sample and kits.
A method is provided for enriching extracellular DNA from a biological sample comprising extracellular DNA and extracellular vesicles, wherein the method comprises: (a) preparing a binding mixture comprising - the biological sample, - a solid phase comprising anion exchange groups, - an acidic binding buffer comprising a buffering agent, and binding extracellular DNA to the solid phase comprising anion exchange groups; (b) separating the solid phase with the bound extracellular DNA from the remaining binding mixture, wherein the remaining binding mixture comprises extracellular vesicles. The method may furthermore comprise processing the remaining binding mixture to enrich one or more biological targets of interest therefrom, wherein processing may comprise (c) enriching as biological targets extracellular vesicles and/or extracellular RNA from the remaining binding mixture.
