A method for identifying a cell line during incubation of the cell line, wherein a database of growth condition data for a cell line is provided. The database is shared in a network. Current growth condition data for a cell line being incubated is obtained and the current growth condition data for the cell line being incubated is compared to previously obtained corresponding growth condition data for the cell line in the database to indicate whether current growth condition data for the cell line is consistent with the previously obtained corresponding growth condition data for the cell line.
G06F 16/2457 - Traitement des requêtes avec adaptation aux besoins de l’utilisateur
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
2.
METHOD AND APPARATUS FOR IMAGING OF CELLS FOR COUNTING CELLS, CONFLUENCE MEASUREMENT AND PLAQUE DETECTION
A method and apparatus for measuring cells and cell confluence includes an imager for imaging a z-stack of bright field images and a processor for selecting a best focus bright field image at a z position of f microns in the z-stack of images. In the manner described herein, the processor selects a bright image n microns lower in the z-stack than the best focus bright field image and a dark image m microns higher in the stack than the best focus bright field image. The processor applies edge detection to the dark image to obtain an edge image and subtracts the edge image from the dark image and the processor applies an adaptive threshold to the bright image to create a mask and uses the adaptive thresholded image as seeds to evaluate the edge image to find cell candidates.
A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
H04W 4/38 - Services spécialement adaptés à des environnements, à des situations ou à des fins spécifiques pour la collecte d’informations de capteurs
H04W 4/80 - Services utilisant la communication de courte portée, p. ex. la communication en champ proche, l'identification par radiofréquence ou la communication à faible consommation d’énergie
4.
Method and system for cell operations using the internet of things
A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
H04W 4/38 - Services spécialement adaptés à des environnements, à des situations ou à des fins spécifiques pour la collecte d’informations de capteurs
H04W 4/80 - Services utilisant la communication de courte portée, p. ex. la communication en champ proche, l'identification par radiofréquence ou la communication à faible consommation d’énergie
5.
METHOD AND APPARATUS FOR SEARCHING AND ANALYZING CELL IMAGES
A method and apparatus for searching cell images comprising storing cell images in an image database, generating index data for the stored images in the image database, wherein the index data includes image metadata and data extracted from the stored image data by analysis including morphological descriptors, applications of algorithms, machine learning and/or data mining.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G16H 30/20 - TIC spécialement adaptées au maniement ou au traitement d’images médicales pour le maniement d’images médicales, p. ex. DICOM, HL7 ou PACS
G16H 50/70 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour extraire des données médicales, p. ex. pour analyser les cas antérieurs d’autres patients
A cell incubator system comprising a housing having an internal chamber having a controlled temperature and gas mixture therein appropriate for the growth of particular cells for incubation of the particular cells in one or more cell culture vessels in the internal chamber and a plurality of cell operation stations in the internal chamber. The stations receive plates having wells for holding cells and wherein the cell operations stations perform operations on cells in the wells. The system has a robotic transport for moving plates from one station to another.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 15/10 - Recherche de particules individuelles
G01N 15/1433 - Traitement du signal utilisant la reconnaissance d’image
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Aspects relate to automated cell culture incubators comprising imaging systems, e.g., holographic or phase-contrast imagers, configured for imaging said cells within the incubators.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
8.
AUTOMATED APPARATUS AND METHOD FOR IN VITRO FERTILIZATION
A method and apparatus for incubating sperm, eggs, and/or embryos for in vitro fertilization placing at least one of sperm, eggs, and embryos in at least one cell culture vessel in an incubator chamber having windows with filters to filter out ultraviolet light, wherein interior surfaces of the chamber comprises material that prevents the growth of fungus, and wherein the chamber includes sensors for regulating the levels of oxygen, carbon dioxide and nitrogen in the chamber, transporting the at least one cell culture vessel from a storage location in the chamber to an imager at an imaging location in the chamber using an elevator; and repeatedly imaging at least one of the sperm, eggs, and the embryos in the imager while maintaining the levels of oxygen, carbon dioxide and nitrogen throughout the chamber.
An improved imaging method and apparatus for the finding of cells, the counting of cells and the determination of the confluence of a cell culture using a phase image of the cells in a cell culture and in some embodiments, the quantitative phase image of a cell culture.
A method and apparatus for quantitative phase imaging a cell culture in a cell culture vessel has an imager for providing z-stack brightfield images of the cell culture in the cell culture vessel. The processor processes the images to determine a plane of z which represents a central focal plane for the images, determines a polynomial function that estimates the intensities at each pixel location in the images as a function of z, creates an intensity image using polynomials from the polynomial function to create an image, creates a first derivative image using polynomials from the polynomial function, uses the intensity image and the first derivative image to define a three-dimensional voxel formed at each corresponding pixel in the intensity image and the first derivative image, and uses a transport of intensity equation to determine a phase distribution of each voxel and its surrounding voxels in order to obtain a quantitative phase image of the cell culture.
A method and apparatus for counting cells in image regions of a phase image of a cell culture in a cell culture vessel, comprising finding the local maximum of cell size of each image region, creating a local threshold value in each cell-sized image region, creating a mask using the threshold value which separates each cell to segment the cells into cell phase regions, creating a contour around the edge of each segmented cell phase region to define a cell outline, creating lists of the cell outlines, wherein each cell has its own outline list of points and shape parameters; and counting the number of cells using the lists. For counting cells in a phase image of a cell culture in a cell culture vessel, wherein at least one processor is configured to find the local maximum of regions of cell size, create a local threshold value in each cell sized region, create a mask using the threshold value which separates each cell to segment the cells, create a contour around the edge of each segmented cell phase region, create lists of the cell outlines, wherein each cell has its own outline list of points and shape parameters and count the number of cells.
A method and system for performing operations on cells on an instrument comprises providing a peer to peer network of instruments for performing operations on cells, maintaining a distributed database in at least a plurality of the instruments in the peer to peer network, storing at least one protocol in the database for use by at least one instrument in the peer to peer network for performing operations on cells and storing results from the performance of the operations on cells by the at least one instrument in the distributed database. The results stored in the distributed database is then usable to later authenticate the results from the at least one instrument.
G06F 16/27 - Réplication, distribution ou synchronisation de données entre bases de données ou dans un système de bases de données distribuéesArchitectures de systèmes de bases de données distribuées à cet effet
G06F 16/907 - Recherche caractérisée par l’utilisation de métadonnées, p. ex. de métadonnées ne provenant pas du contenu ou de métadonnées générées manuellement
G06F 21/62 - Protection de l’accès à des données via une plate-forme, p. ex. par clés ou règles de contrôle de l’accès
13.
PLAQUE DETECTION METHOD AND APPARATUS FOR IMAGING OF CELLS
A method and system comprise taking a series of time spaced images of a cell culture having pathogens therein creating plaques, applying a stain to the cell culture, taking an image of the stained plaques, using the image of the stained culture to build a model of the plaques in earlier pre-stain images of the culture and displaying the pre-stain images and identifying the plaques therein based upon the model.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
A cell culture incubator has an incubator cabinet with an internal chamber for the incubation of cells in one or more cell culture vessels and a door opening from an external environment to the internal chamber. At least two imagers configured for imaging the cells are within the internal chamber and a manipulator for manipulating the cells in the one or more cell culture vessels is within the internal chamber. A cell culture vessel transfer device moves the one or more cell culture vessels between the at least two imagers within the internal chamber and a controller is configured for controlling the cell culture transfer device to move cell culture vessels between the at least two imagers.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 15/1433 - Traitement du signal utilisant la reconnaissance d’image
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 15/10 - Recherche de particules individuelles
Aspects relate to an automated cell culture incubator and to methods for using such an incubator. In one aspect, the cell culture incubator includes an incubator cabinet having a transfer chamber and an internal chamber that are arranged to form an airlock configuration. In some embodiments, objects placed into the incubator cabinet are sterilized using ozone produced by an ozone generator. The incubator may include one or more transfer devices that move objects between the transfer chamber and the internal chamber.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
16.
METHOD AND APPARATUS FOR CALIBRATING AN IMAGING SYSTEM
A method and apparatus for calibrating an imaging system provides a program stored in the imaging system having a graphical user interface and providing a plurality of tools to align hardware components of the imaging system including cameras for imaging cell culture vessels received by the imaging system and having a tool for providing a video image output of a cell culture vessel received in the imaging system.
In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 3/02 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des suspensions
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
18.
METHOD AND SYSTEM FOR PERFORMING CELL OPERATIONS USING A VERIFIABLE DATA STRUCTURE
A method and system for performing operations on cells in an instrument wherein the performance of operations on cell samples is controlled and data relating to the operations is generated, a unique identifier is associated with the instrument, the generated data is encrypted with a key corresponding to the unique identifier and a hash of one of the generated data and the encrypted generated data is created to enable verification of the integrity thereof. The hash is stored for later verification of data.
H04L 9/32 - Dispositions pour les communications secrètes ou protégéesProtocoles réseaux de sécurité comprenant des moyens pour vérifier l'identité ou l'autorisation d'un utilisateur du système
A device, method and apparatus for controlling humidity in a cell incubator. The device includes a thermally conductive block having a water exuding surface, an electrical heater attached to the block and receptive of electrical energy to heat the block, at least one fan for blowing gas to be humidified over the surface of the block, at least humidity sensor attached at least downwind of the block, a controllable pump connectable to a source of water for supplying water to the exuding surface of the block and a controller receptive of output signals from the at least one humidity sensor and connected to the pump for controlling the pump based upon the output signals from the at least one humidity sensor to obtain a desired level of humidity.
Method and apparatus for performing a plaque detection by using above focus and below focus images. Plaque is also detected by a method and apparatus using test and training data captured on an imaging system, building a new model for a specific virus/cell/protocol type to detect plaques, using the models in runtime systems to detect plaques and augmenting the models based on automatically calculated false positive and false negative counts and percentages taken from test runs and/or runtime data.
A method for improving the illumination of a three-dimensional cellular structure such as an organoid or spheroid for imaging, comprising the steps of: culturing the structure with a scaffold; and directing light onto the structure using the scaffold. Also disclosed is a method for improving the illumination of a three-dimensional cellular structure such as an organoid or spheroid for imaging, comprising the steps of: culturing the structure; and directing light into the structure using a light source. Further disclosed is a method for improving the imaging of a three dimensional cellular structure such as an organoid or spheroid comprising the steps of applying waves to the structure and measuring the waves to create an image of the structure.
Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
23.
METHOD AND APPARATUS FOR PREVENTING CELL CULTURE PLATE CONDENSATION IN AN IMAGING SYSTEM
A method and apparatus for preventing condensation on a cell culture plate transferred from an incubator to an imager. A block having a higher thermal conductivity layer and a lower thermal conductivity layer is preheated in an incubator. The block is placed on a cell culture plate either before or immediately after removing the cell culture plate from an incubator with the higher thermal conductivity layer resting on the plate and configured to overlie all of the wells of the plate. The culture plate is transported to an imager with the thermal block in place and the block is removed before inserting the culture plate into the imager.
A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.
Aspects of the present invention relate to an automated cell culture instrument and to methods for operating such an instrument. In one aspect, the cell culture instrument includes a computing unit and a plurality of electrically controllable resources (e.g., sensors, actuators, etc.). The computing unit receives a protocol, schedules the resources so as to execute the protocol, and operates the resources as scheduled or in response to so as to provide an improved environment for cell culture growth. The computing unit may simulate protocol execution in connection with the scheduling or execution of the protocol.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G06F 9/50 - Allocation de ressources, p. ex. de l'unité centrale de traitement [UCT]
G06N 7/00 - Agencements informatiques fondés sur des modèles mathématiques spécifiques
According to at least one aspect, a system configured to count cells in a vessel is provided. The system comprises an imaging system configured to image cells in the vessel and a controller coupled to the imaging system. The controller is configured to control the imaging system to capture a focused image of the cells and estimate a number of cells in the focused image. The controller is configured to control the imaging system to capture a focused image of the cells at least in part by controlling the imaging system to capture a plurality of images of the cells in a plurality of focal planes, determining an area of at least one cell in each of the plurality of images, and selecting one image from the plurality of images as the focused image using the area of the at least one cell in the plurality of images.
A method and apparatus system for determining a depth of media in a cell culture vessel well wherein at least one calibration cell culture vessel with different predetermined depths of media in each well is provided. An imager is used for imaging each of the calibration wells and storing the calibration images thereof. An image processor correlates the calibration images to the different predetermined media depths. The imager images a cell culture vessel with at least one unknown media depth and the image processor compares the image of the at least one unknown media depth to the calibration images and determining the at least one unknown media depth from the correlation to the different predetermined media depths.
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
29.
CELL INCUBATOR AND A TOOL FOR USE IN A CELL INCUBATOR
At least one tool removably mountable on a cell incubator mandrel or a cell incubator having at least one mandrel having an end portion, the at least one mandrel mounted on a movable transport for moving the at least one mandrel and at the least one tool removably mountable on the end portion of the at least one mandrel. The at least one tool is preferably electrically powered and controllable by signals applied to the at least one tool to perform at least one operation with respect to the incubation of cells.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
30.
METHOD AND APPARATUS FOR DISINFECTING LABORATORY INSTRUMENTS
A method and apparatus for disinfecting a laboratory instrument having a housing with pathogens therein. A plurality of UVC light sources are provided in the housing and the pathogens in the housing are moved relative to the light from the UVC light sources.
A61L 2/10 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des phénomènes physiques des radiations des ultraviolets
Methods for the image processing od cells are disclosed for the purpose of segmenting cell colonies for further processing to determine confluence, counting and morphology.
Method and apparatus for displaying cultured cell wherein the image can be smoothly moved into and out of focus and can be zoomed in and out and panned.
A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
H04W 4/38 - Services spécialement adaptés à des environnements, à des situations ou à des fins spécifiques pour la collecte d’informations de capteurs
H04W 4/80 - Services utilisant la communication de courte portée, p. ex. la communication en champ proche, l'identification par radiofréquence ou la communication à faible consommation d’énergie
34.
Method and system for cell operations using the internet of things
A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
H04W 4/38 - Services spécialement adaptés à des environnements, à des situations ou à des fins spécifiques pour la collecte d’informations de capteurs
H04W 4/80 - Services utilisant la communication de courte portée, p. ex. la communication en champ proche, l'identification par radiofréquence ou la communication à faible consommation d’énergie
Aspects of the invention relate to liquid transfer systems including a pipette tip having a body defining an inner volume and having first and second ends, the first end arranged to transfer a volume of liquid into and out of the pipette tip and the second end arranged for attachment to a pipette, and a bladder sealingly attached to the body. In some embodiments, the bladder is disposable in the inner volume. The bladder may be defined by a membranous sac and/or a planar membrane. The bladder may be moveable in response to an applied pressure to transfer liquid. The bladder also may be stretchable to accommodate a volume of fluid. In some embodiments, the pipette tip is used in a cell culture incubator, with a manipulator including a pipette that is in fluid communication with the pipette tip.
A method and apparatus for incubating sperm, eggs, and/or embryos for in vitro fertilization placing at least one of sperm, eggs, and embryos in at least one cell culture vessel in an incubator chamber having windows with filters to filter out ultraviolet light, wherein interior surfaces of the chamber comprises material that prevents the growth of fungus, and wherein the chamber includes sensors for regulating the levels of oxygen, carbon dioxide and nitrogen in the chamber, transporting the at least one cell culture vessel from a storage location in the chamber to an imager at an imaging location in the chamber using an elevator; and repeatedly imaging at least one of the sperm, eggs, and the embryos in the imager while maintaining the levels of oxygen, carbon dioxide and nitrogen throughout the chamber.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
A system for dissociating cells from a cell culture vessel. The system comprises an imaging system configured to image a plurality of cells in a cell culture vessel being dissociated from at least one surface of the cell culture vessel by at least one cell dissociation agent; and at least one controller coupled to the imaging system and configured to: control the imaging system to capture a sequence of images of at least some cells in the plurality of cells during dissociation; and identify when to neutralize the at least one cell dissociation agent using the sequence of images.
G01N 15/1433 - Traitement du signal utilisant la reconnaissance d’image
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
fnmm microns higher in the stack than the best focus bright field image wherein the cells as a whole are darker than the background and have a high contrast boundary. The at least one processor applies edge detection to the dark image to obtain an edge image and subtracts the edge image from the dark image and the at least one processor applies an adaptive threshold to the bright image to create a mask based upon the dots in the cells and uses the adaptive thresholded image as seeds to evaluate the edge image with a watershed analysis to find cell candidates.
Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
40.
METHOD AND APPARATUS FOR SEARCHING AND ANALYZING CELL IMAGES
A method and apparatus for searching cell images comprising storing cell images in an image database, generating index data for the stored images in the image database, wherein the index data includes image metadata and data extracted from the stored image data by analysis including morphological descriptors, applications of algorithms, machine learning and/or data mining.
A method for pre-scanning a transparent cell culture plate with a plurality of wells to improve focus using a plurality of z-axis images and a method for focusing an optical system on a transparent cell culture plate by performing a Fourier transform on image data at different focus distance steps to reveal a pattern and using the pattern at each step to determine the focus distance.
A method and apparatus for calibrating an imaging system provides a program stored in the imaging system having a graphical user interface and providing a plurality of tools to align hardware components of the imaging system including cameras for imaging cell culture vessels received by the imaging system and having a tool for providing a video image output of a cell culture vessel received in the imaging system.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G02B 21/36 - Microscopes aménagés pour la photographie ou la projection
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
43.
PLAQUE DETECTION METHOD AND APPARATUS FOR IMAGING OF CELLS
A method and system comprise taking a series of time spaced images of a cell culture having pathogens therein creating plaques, applying a stain to the cell culture, taking an image of the stained plaques, using the image of the stained culture to build a model of the plaques in earlier pre-stain images of the culture and displaying the pre-stain images and identifying the plaques therein based upon the model.
Aspects of the invention relate to cell culture vessels and method for using the vessels to agitate and maintain cells in suspension. In some embodiments, the vessel has a body configured to hold a volume of liquid containing cells to be cultured, the vessel body having a deformable portion arranged to induce movement of the liquid sufficient to maintain the cells in suspension. In some embodiments, the deformable portion is in at least one of a base and sidewall of the vessel body. In some embodiments, the deformable portion is deformed according to a deformable pattern. In some embodiments, the deformable portion is deformed via a deformation plate having one or more movable members that contact the deformable portion.
A method and system for performing operations on cells on an instrument comprises providing a peer to peer network of instruments for performing operations on cells, maintaining a distributed database in at least a plurality of the instruments in the peer to peer network, storing at least one protocol in the database for use by at least one instrument in the peer to peer network for performing operations on cells and storing results from the performance of the operations on cells by the at least one instrument in the distributed database. The results stored in the distributed database is then usable to later authenticate the results from the at least one instrument.
G06F 16/27 - Réplication, distribution ou synchronisation de données entre bases de données ou dans un système de bases de données distribuéesArchitectures de systèmes de bases de données distribuées à cet effet
G06F 16/907 - Recherche caractérisée par l’utilisation de métadonnées, p. ex. de métadonnées ne provenant pas du contenu ou de métadonnées générées manuellement
G06F 21/62 - Protection de l’accès à des données via une plate-forme, p. ex. par clés ou règles de contrôle de l’accès
46.
DEVICE, METHOD AND APPARATUS FOR CONTROLLING HUMIDITY IN AN INCUBATOR
A device, method and apparatus for controlling humidity in a cell incubator. The device includes a thermally conductive block having a water exuding surface, an electrical heater attached to the block and receptive of electrical energy to heat the block, at least one fan for blowing gas to be humidified over the surface of the block, at least humidity sensor attached at least downwind of the block, a controllable pump connectable to a source of water for supplying water to the exuding surface of the block and a controller receptive of output signals from the at least one humidity sensor and connected to the pump for controlling the pump based upon the output signals from the at least one humidity sensor to obtain a desired level of humidity.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G05D 22/02 - Commande de l'humidité caractérisée par l'utilisation de moyens électriques
Method and apparatus for performing a plaque detection by using above focus and below focus images. Plaque is also detected by a method and apparatus using test and training data captured on an imaging system, building a new model for a specific virus/cell/protocol type to detect plaques, using the models in runtime systems to detect plaques and augmenting the models based on automatically calculated false positive and false negative counts and percentages taken from test runs and/or runtime data.
A method and system for performing operations on cells on an instrument wherein a network of instruments for performing operations on cells is provided. The operations are controlled by protocols run on the instruments and data relating to the protocols is metadata. A database of protocols and metadata relating to the protocols is maintained and results from the performance of the operations on cells by the instruments in the network is stored in the database. The results for the protocols and metadata are analyzed to vary at least one of the stored protocols and the stored metadata to improve future results.
A method and system for performing operations on cells in an instrument wherein the performance of operations on cell samples is controlled and data relating to the operations is generated, a unique identifier is associated with the instrument, the generated data is encrypted with a key corresponding to the unique identifier and a hash of one of the generated data and the encrypted generated data is created to enable verification of the integrity thereof. The hash is stored for later verification of data.
G06F 21/62 - Protection de l’accès à des données via une plate-forme, p. ex. par clés ou règles de contrôle de l’accès
H04L 9/12 - Dispositifs de chiffrement d'émission et de réception synchronisés ou initialisés d'une manière particulière
H04L 9/32 - Dispositions pour les communications secrètes ou protégéesProtocoles réseaux de sécurité comprenant des moyens pour vérifier l'identité ou l'autorisation d'un utilisateur du système
50.
A METHOD FOR IDENTIFYING A CELL LINE DURING INCUBATION OF THE CELL LINE
A method for identifying a cell line during incubation of the cell line, wherein a database of growth condition data for a cell line is provided. The database is shared in a network. Current growth condition data for a cell line being incubated is obtained and the current growth condition data for the cell line being incubated is compared to previously obtained corresponding growth condition data for the cell line in the database to indicate whether current growth condition data for the cell line is consistent with the previously obtained corresponding growth condition data for the cell line.
A cell incubator system comprising a housing having an internal chamber having a controlled temperature and gas mixture therein appropriate for the growth of particular cells for incubation of the particular cells in one or more cell culture vessels in the internal chamber and a plurality of cell operation stations in the internal chamber. The stations receive plates having wells for holding cells and wherein the cell operations stations perform operations on cells in the wells. The system has a robotic transport for moving plates from one station to another.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
52.
METHOD AND APPARATUS FOR ILLUMINATING AND IMAGING ORGANOIDS AND SPHEROIDS
A method for improving the illumination of a three-dimensional cellular structure such as an organoid or spheroid for imaging, comprising the steps of: culturing the structure with a scaffold; and directing light onto the structure using the scaffold. Also disclosed is a method for improving the illumination of a three-dimensional cellular structure such as an organoid or spheroid for imaging, comprising the steps of: culturing the structure; and directing light into the structure using a light source. Further disclosed is a method for improving the imaging of a three dimensional cellular structure such as an organoid or spheroid comprising the steps of applying waves to the structure and measuring the waves to create an image of the structure.
Aspects relate to an automated cell culture incubator and to methods for using such an incubator. In one aspect, the cell culture incubator includes an incubator cabinet having a transfer chamber and an internal chamber that are arranged to form an airlock configuration. In some embodiments, objects placed into the incubator cabinet are sterilized using ozone produced by an ozone generator. The incubator may include one or more transfer devices that move objects between the transfer chamber and the internal chamber.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.
A cell culture incubator with an incubator cabinet has an internal chamber with a door opening from an external environment. An imager and a manipulator with a scraper are in the internal chamber. A controller is configured for controlling the manipulator to modulate the contact pressure between a scraping edge of the cell scraper and a surface of a cell culture vessel and a sensor is connected to the cell scraper that provides signal to the controller informative of a sensed pressure between a scraping edge of the cell scraper and the surface of a cell culture vessel. The controller controls the manipulator to increase or decrease the pressure between a scraping edge of the cell scraper and the surface of a cell culture vessel.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 15/10 - Recherche de particules individuelles
A method and apparatus for disinfecting a laboratory instrument having a housing with pathogens therein. A plurality of UVC light sources are provided in the housing and the pathogens in the housing are moved relative to the light from the UVC light sources.
A61L 2/00 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet
A61L 2/10 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des phénomènes physiques des radiations des ultraviolets
57.
METHOD AND APPARATUS FOR PREVENTING CELL CULTURE PLATE CONDENSATION IN AN IMAGING SYSTEM
A method and apparatus for preventing condensation on a cell culture plate transferred from an incubator to an imager. A block having a higher thermal conductivity layer and a lower thermal conductivity layer is preheated in an incubator. The block is placed on a cell culture plate either before or immediately after removing the cell culture plate from an incubator with the higher thermal conductivity layer resting on the plate and configured to overlie all of the wells of the plate. The culture plate is transported to an imager with the thermal block in place and the block is removed before inserting the culture plate into the imager.
A plaque assay counting method, comprising the steps of: placing cells in wells of a plate, applying virions on the cells, thereafter imaging the virus infected cells at desired intervals without having to kill the still living cells and fixing same between the desired intervals resulting in a time sequence of images and using the time sequence of images to identify plaques for counting.
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
C12N 7/00 - Virus, p. ex. bactériophagesCompositions les contenantLeur préparation ou purification
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
59.
METHOD AND APPARATUS FOR DISPLAYING CULTURED CELLS
Method and apparatus for displaying cultured cell wherein the image can be smoothly moved into and out of focus and can be zoomed in and out and panned.
A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
H04W 4/80 - Services utilisant la communication de courte portée, p. ex. la communication en champ proche, l'identification par radiofréquence ou la communication à faible consommation d’énergie
In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/02 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des suspensions
62.
Method and system for cell operations using the internet of things
A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
H04W 4/80 - Services utilisant la communication de courte portée, p. ex. la communication en champ proche, l'identification par radiofréquence ou la communication à faible consommation d’énergie
A method for processing an image of cell colonies to segment the cell colonies, comprising the steps of: providing a gray scale image of cell colonies comprising a plurality of pixels; remapping the image; centering a mask over each pixel in the remapped image for creating a gray scale histogram; calculating a number of bins in each histogram, wherein the total number of bins is less than required by the gray scale range; assigning the calculated number of bins for a pixel as a value for that pixel in the remapped image; thresholding the remapped image pixel values to produce a binary image wherein the cell colonies are segmented.
G06K 9/00 - Méthodes ou dispositions pour la lecture ou la reconnaissance de caractères imprimés ou écrits ou pour la reconnaissance de formes, p.ex. d'empreintes digitales
A61B 5/00 - Mesure servant à établir un diagnostic Identification des individus
A61B 10/02 - Instruments pour prélever des échantillons cellulaires ou pour la biopsie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12Q 1/24 - Méthodes d'échantillonnage, d'inoculation ou de développement d'un échantillonMéthodes pour isoler physiquement un micro-organisme intact
Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Cell culture media for cell plating, cell sorting and bioreactors for cell feeding for scientific and research use Medical research apparatus and instruments for collecting and analyzing cell data for laboratory use, namely, incubators for cell cultures, cameras, microscopes, flow cytometers and flow-based analyzers for providing cell analysis, detection, counting, and imaging, dropping pipettes for cell passaging, and computers for cell evaluation, all of the foregoing for use in conjunction with individual cells, colonies of cells, stem cells, tissue cells, combinations of cells, co-cultures in the nature of cell cultures containing two or more different types of cells, organoids in the nature of self-organized three-dimensional stem cell derived cell tissue cultures, spheroids in the nature of three-dimensional cell cultures that arrange during proliferation into sphere-like formations, assembloids in the nature of three dimensional structures formed from the fusion and functional integration of multiple cell types, and embryonic cells; pharmaceutical apparatus and instruments, namely, electronic apparatus for testing the sterility of pharmaceuticals and injectable solutions; diagnostic apparatus for the detection of pathogens, genetic mutations, diseases or infections for laboratory and research use; downloadable computer software for collecting and analyzing data on cells for laboratory, research, clinical, diagnostic or medical use, for cell imaging, cell counting, cell sorting, cell evaluation, detection of pathogens, genetic mutations, diseases or infections, monitoring of cell response to drugs and pathogens, measuring and evaluating cell response and resistance to drugs and pathogens, for use in conjunction with individual cells, colonies of cells, stem cells, tissue cells, combinations of cells, co-cultures in the nature of cell cultures containing two or more different types of cells, organoids in the nature of self-organized three-dimensional stem cell derived cell tissue cultures, spheroids in the nature of three-dimensional cell cultures that arrange during proliferation into sphere-like formations, assembloids in the nature of three dimensional structures formed from the fusion and functional integration of multiple cell types, and embryonic cells Medical apparatus for introducing pharmaceutical preparations into the human body; clinical medical diagnostic instruments for the analysis of body fluids; diagnostic apparatus for the detection of pathogens, genetic mutations, diseases or infections for medical use
66.
Method and system for cell operations using artificial intelligence
A method and system for performing operations on cells on an instrument wherein a network of instruments for performing operations on cells is provided. The operations are controlled by protocols run on the instruments and data relating to the protocols is metadata. A database of protocols and metadata relating to the protocols is maintained and results from the performance of the operations on cells by the instruments in the network is stored in the database. The results for the protocols and metadata are analyzed to vary at least one of the stored protocols and the stored metadata to improve future results.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G16C 20/90 - Langages de programmationArchitectures informatiquesSystèmes de bases de donnéesStockage de données
G16C 20/70 - Apprentissage automatique, exploration de données ou chimiométrie
G16C 20/10 - Analyse ou conception des réactions, des synthèses ou des procédés chimiques
67.
Method and system for identifying a cell line during incubation of the cell line
A method for identifying a cell line during incubation of the cell line, wherein a database of growth condition data for a cell line is provided. The database is shared in a network. Current growth condition data for a cell line being incubated is obtained and the current growth condition data for the cell line being incubated is compared to previously obtained corresponding growth condition data for the cell line in the database to indicate whether current growth condition data for the cell line is consistent with the previously obtained corresponding growth condition data for the cell line.
G06F 16/2457 - Traitement des requêtes avec adaptation aux besoins de l’utilisateur
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
A method for pre-scanning a transparent cell culture plate with a plurality of wells to improve focus using a plurality of z-axis images and a method for focusing an optical system on a transparent cell culture plate by performing a Fourier transform on image data at different focus distance steps to reveal a pattern and using the pattern at each step to determine the focus distance.
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
G01N 21/3577 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière infrarouge pour l'analyse de liquides, p. ex. l'eau polluée
Aspects of the present invention relate to an automated cell culture instrument and to methods for operating such an instrument. In one aspect, the cell culture instrument includes a computing unit and a plurality of electrically controllable resources (e.g., sensors, actuators, etc.). The computing unit receives a protocol, schedules the resources so as to execute the protocol, and operates the resources as scheduled or in response to so as to provide an improved environment for cell culture growth. The computing unit may simulate protocol execution in connection with the scheduling or execution of the protocol.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G06F 9/50 - Allocation de ressources, p. ex. de l'unité centrale de traitement [UCT]
G06N 7/00 - Agencements informatiques fondés sur des modèles mathématiques spécifiques
70.
CELL CULTURE INCUBATORS WITH INTEGRATED IMAGING SYSTEMS
Aspects relate to automated cell culture incubators comprising imaging systems, e.g., holographic or phase-contrast imagers, configured for imaging said cells within the incubators.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
In some aspects, the invention relates to selectively permeable cell culture vessel storage containers and their methods of use. In some embodiments, the containers comprise a gas-permeable membrane that is selectively impermeable to water vapor.
A cell incubator system comprising a housing having an internal chamber having a controlled temperature and gas mixture therein appropriate for the growth of particular cells for incubation of the particular cells in one or more cell culture vessels in the internal chamber and a plurality of cell operation stations in the internal chamber. The stations receive plates having wells for holding cells and wherein the cell operations stations perform operations on cells in the wells. The system has a robotic transport for moving plates from one station to another.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 15/1433 - Traitement du signal utilisant la reconnaissance d’image
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 15/10 - Recherche de particules individuelles
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Cell culture media for cell plating, cell sorting and bioreactors for cell feeding for scientific and research use Medical research apparatus and instruments for collecting and analyzing cell data for laboratory use, namely, incubators for cell cultures, cameras, microscopes, flow cytometers and flow-based analyzers for providing cell analysis, detection, counting, and imaging, dropping pipettes for cell passaging, and computers for cell evaluation, all of the foregoing for use in conjunction with individual cells, colonies of cells, stem cells, tissue cells, combinations of cells, co-cultures in the nature of cell cultures containing two or more different types of cells, organoids in the nature of self-organized three-dimensional stem cell derived cell tissue cultures, spheroids in the nature of three-dimensional cell cultures that arrange during proliferation into sphere-like formations, assembloids in the nature of three dimensional structures formed from the fusion and functional integration of multiple cell types, and embryonic cells; pharmaceutical apparatus and instruments, namely, electronic apparatus for testing the sterility of pharmaceuticals and injectable solutions; diagnostic apparatus for the detection of pathogens, genetic mutations, diseases or infections for laboratory and research use; downloadable computer software for collecting and analyzing data on cells for laboratory, research, clinical, diagnostic or medical use, for cell imaging, cell counting, cell sorting, cell evaluation, detection of pathogens, genetic mutations, diseases or infections, monitoring of cell response to drugs and pathogens, measuring and evaluating cell response and resistance to drugs and pathogens, for use in conjunction with individual cells, colonies of cells, stem cells, tissue cells, combinations of cells, co-cultures in the nature of cell cultures containing two or more different types of cells, organoids in the nature of self-organized three-dimensional stem cell derived cell tissue cultures, spheroids in the nature of three-dimensional cell cultures that arrange during proliferation into sphere-like formations, assembloids in the nature of three dimensional structures formed from the fusion and functional integration of multiple cell types, and embryonic cells Medical apparatus for introducing pharmaceutical preparations into the human body; clinical medical diagnostic instruments for the analysis of body fluids; diagnostic apparatus for the detection of pathogens, genetic mutations, diseases or infections for medical use
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Apparatus and instruments for collecting and analyzing data on cells, including but not limited to individual cells, colonies of cells, stem cells, tissues, combinations of cells, co-cultures, namely cell cultures containing two or more different types of cells, organoids, namely, tiny, self-organized three-dimensional tissue cultures that are derived from stem cells, spheroids, namely, three-dimensional cell cultures that arrange themselves during proliferation into sphere-like formations, assembloids, namely, three dimensional structures formed from the fusion and functional integration of multiple cell types and embryos, for laboratory and research, namely, incubators for cell cultures, microscopes for cell imaging, cameras for cell imaging, computers for cell analysis, cell counting apparatus, cell culture media for cell sorting and bioreactors for cell feeding, laboratory pipettes for cell passaging, cell culture media for cell plating, and computers for cell evaluation; pharmaceutical apparatus and instruments, namely, electronic apparatus for testing the sterility of pharmaceuticals and injectable solutions; diagnostic apparatus for the detection of pathogens, genetic mutations, diseases or infections for laboratory and research use; downloadable computer software for collecting and analyzing data on cells for laboratory, research, clinical, diagnostic or medical use, for cell imaging, cell counting, cell sorting, cell evaluation, detection of pathogens, genetic mutations, diseases or infections, monitoring of cell response to drugs and pathogens, measuring and evaluating cell response and resistance to drugs and pathogens, where cells, include but are not limited to individual cells, colonies of cells, stem cells, tissues, combinations of cells, co-cultures, namely cell cultures containing two or more different types of cells, organoids, namely, tiny, self-organized three-dimensional tissue cultures that are derived from stem cells, spheroids, namely, three-dimensional cell cultures that arrange themselves during proliferation into sphere-like formations, assembloids, namely, three dimensional structures formed from the fusion and functional integration of multiple cell types Apparatus and instruments for collecting and analyzing data on cells, including but not limited to individual cells, colonies of cells, stem cells, tissues, combinations of cells, co-cultures, namely cell cultures containing two or more different types of cells, organoids, namely, tiny, self-organized three-dimensional tissue cultures that are derived from stem cells, spheroids, namely, three-dimensional cell cultures that arrange themselves during proliferation into sphere-like formations, assembloids, namely, three dimensional structures formed from the fusion and functional integration of multiple cell types and embryos, for medical use, namely, incubators for cell cultures, cameras, microscopes, flow cytometers and flow-based analyzers providing cell analysis, detection, counting, cell imaging, cell culture media for cell sorting and dropping pipettes for cell passaging, cell culture media for cell plating, and computers for cell evaluation; medical apparatus for introducing pharmaceutical preparations into the human body; clinical medical diagnostic instruments for the analysis of body fluids; diagnostic apparatus for the detection of pathogens, genetic mutations, diseases or infections for medical use
75.
Cell culture incubators with integrated cell manipulation systems
Aspects of the invention relate to automated cell culture incubators. In some embodiments, automated cell culture incubators comprise an integrated manipulation device having one or more cell scrapers.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 15/10 - Recherche de particules individuelles
A system for dissociating cells from a cell culture vessel. The system comprises an imaging system configured to image a plurality of cells in a cell culture vessel being dissociated from at least one surface of the cell culture vessel by at least one cell dissociation agent; and at least one controller coupled to the imaging system and configured to: control the imaging system to capture a sequence of images of at least some cells in the plurality of cells during dissociation; and identify when to neutralize the at least one cell dissociation agent using the sequence of images.
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G16B 50/30 - Entreposage de donnéesArchitectures informatiques
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G02B 21/32 - Micromanipulateurs combinés par construction avec des microscopes
A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
H04W 4/80 - Services utilisant la communication de courte portée, p. ex. la communication en champ proche, l'identification par radiofréquence ou la communication à faible consommation d’énergie
At least one tool removably mountable on a cell incubator mandrel or a cell incubator having at least one mandrel having an end portion, the at least one mandrel mounted on a movable transport for moving the at least one mandrel and at the least one tool removably mountable on the end portion of the at least one mandrel. The at least one tool is preferably electrically powered and controllable by signals applied to the at least one tool to perform at least one operation with respect to the incubation of cells.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
79.
Method and system for cell operations using a blockchain data network
A method and system for performing operations on cells on an instrument comprises providing a peer to peer network of instruments for performing operations on cells, maintaining a distributed database in at least a plurality of the instruments in the peer to peer network, storing at least one protocol in the database for use by at least one instrument in the peer to peer network for performing operations on cells and storing results from the performance of the operations on cells by the at least one instrument in the distributed database. The results stored in the distributed database is then usable to later authenticate the results from the at least one instrument.
G06F 16/27 - Réplication, distribution ou synchronisation de données entre bases de données ou dans un système de bases de données distribuéesArchitectures de systèmes de bases de données distribuées à cet effet
G06F 21/62 - Protection de l’accès à des données via une plate-forme, p. ex. par clés ou règles de contrôle de l’accès
G06F 16/907 - Recherche caractérisée par l’utilisation de métadonnées, p. ex. de métadonnées ne provenant pas du contenu ou de métadonnées générées manuellement
According to at least one aspect, a system configured to count cells in a vessel is provided. The system comprises an imaging system configured to image cells in the vessel and a controller coupled to the imaging system. The controller is configured to control the imaging system to capture a focused image of the cells and estimate a number of cells in the focused image. The controller is configured to control the imaging system to capture a focused image of the cells at least in part by controlling the imaging system to capture a plurality of images of the cells in a plurality of focal planes, determining an area of at least one cell in each of the plurality of images, and selecting one image from the plurality of images as the focused image using the area of the at least one cell in the plurality of images.
Aspects of the invention relate to liquid transfer systems including a pipette tip having a body defining an inner volume and having first and second ends, the first end arranged to transfer a volume of liquid into and out of the pipette tip and the second end arranged for attachment to a pipette, and a bladder sealingly attached to the body. In some embodiments, the bladder is disposable in the inner volume. The bladder may be defined by a membranous sac and/or a planar membrane. The bladder may be moveable in response to an applied pressure to transfer liquid. The bladder also may be stretchable to accommodate a volume of fluid. In some embodiments, the pipette tip is used in a cell culture incubator, with a manipulator including a pipette that is in fluid communication with the pipette tip.
At least one tool removably mountable on a cell incubator mandrel or a cell incubator having at least one mandrel having an end portion, the at least one mandrel mounted on a movable transport for moving the at least one mandrel and at the least one tool removably mountable on the end portion of the at least one mandrel. The at least one tool is preferably electrically powered and controllable by signals applied to the at least one tool to perform at least one operation with respect to the incubation of cells.
Aspects relate to automated cell culture incubators comprising imaging systems, e.g., holographic or phase-contrast imagers, configured for imaging said cells within the incubators.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G03H 1/00 - Procédés ou appareils holographiques utilisant la lumière, les infrarouges ou les ultraviolets pour obtenir des hologrammes ou pour en obtenir une imageLeurs détails spécifiques
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
Aspects of the invention relate to liquid transfer systems including a pipette tip having a body defining an inner volume and having first and second ends, the first end arranged to transfer a volume of liquid into and out of the pipette tip and the second end arranged for attachment to a pipette, and a bladder sealingly attached to the body. In some embodiments, the bladder is disposable in the inner volume. The bladder may be defined by a membranous sac and/or a planar membrane. The bladder may be moveable in response to an applied pressure to transfer liquid. The bladder also may be stretchable to accommodate a volume of fluid. In some embodiments, the pipette tip is used in a cell culture incubator, with a manipulator including a pipette that is in fluid communication with the pipette tip.
B01L 9/00 - Dispositifs de supportDispositifs de serrage
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
According to at least one aspect, a system configured to count cells in a vessel is provided. The system comprises an imaging system configured to image cells in the vessel and a controller coupled to the imaging system. The controller is configured to control the imaging system to capture a focused image of the cells and estimate a number of cells in the focused image. The controller is configured to control the imaging system to capture a focused image of the cells at least in part by controlling the imaging system to capture a plurality of images of the cells in a plurality of focal planes, determining an area of at least one cell in each of the plurality of images, and selecting one image from the plurality of images as the focused image using the area of the at least one cell in the plurality of images.
A system for dissociating cells from a cell culture vessel. The system comprises an imaging system configured to image a plurality of cells in a cell culture vessel being dissociated from at least one surface of the cell culture vessel by at least one cell dissociation agent; and at least one controller coupled to the imaging system and configured to: control the imaging system to capture a sequence of images of at least some cells in the plurality of cells during dissociation; and identify when to neutralize the at least one cell dissociation agent using the sequence of images.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
G01N 15/10 - Recherche de particules individuelles
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 21/41 - RéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique
Aspects of the present invention relate to an automated cell culture instrument and to methods for operating such an instrument. In one aspect, the cell culture instrument includes a computing unit and a plurality of electrically controllable resources (e.g., sensors, actuators, etc.). The computing unit receives a protocol, schedules the resources so as to execute the protocol, and operates the resources as scheduled or in response to so as to provide an improved environment for cell culture growth. The computing unit may simulate protocol execution in connection with the scheduling or execution of the protocol.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G06F 9/50 - Allocation de ressources, p. ex. de l'unité centrale de traitement [UCT]
G06N 7/00 - Agencements informatiques fondés sur des modèles mathématiques spécifiques
Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
Aspects relate to an automated cell culture incubator and to methods for using such an incubator. In one aspect, the cell culture incubator includes an incubator cabinet having a transfer chamber and an internal chamber that are arranged to form an airlock configuration. In some embodiments, objects placed into the incubator cabinet are sterilized using ozone produced by an ozone generator. The incubator may include one or more transfer devices that move objects between the transfer chamber and the internal chamber.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
90.
Cell culture incubators with integrated cell manipulation systems
Aspects of the invention relate to automated cell culture incubators. In some embodiments, automated cell culture incubators comprise an integrated manipulation having one or more cell scrapers.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 15/10 - Recherche de particules individuelles
In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
Aspects of the invention relate to cell culture vessels and method for using the vessels to agitate and maintain cells in suspension. In some embodiments, the vessel has a body configured to hold a volume of liquid containing cells to be cultured, the vessel body having a deformable portion arranged to induce movement of the liquid sufficient to maintain the cells in suspension. In some embodiments, the deformable portion is in at least one of a base and sidewall of the vessel body. In some embodiments, the deformable portion is deformed according to a deformable pattern. In some embodiments, the deformable portion is deformed via a deformation plate having one or more moveable members that contact the deformable portion.
C12N 5/02 - Propagation de cellules individuelles ou de cellules en suspensionLeur conservationMilieux de culture à cet effet
C12M 3/02 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des suspensions
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/10 - Appareillage pour l'enzymologie ou la microbiologie montés rotativement
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
09 - Appareils et instruments scientifiques et électriques
Produits et services
Cell culture apparatus for laboratory use, namely, incubators for cell and tissue cultures, parts and fittings for the aforesaid goods, cell culture dishes, cell culture flasks, cell culture multi-plates, cell culture inserts, cell analysis equipment, cell counting apparatus, cell sorting apparatus, cell feeding apparatus, cell passaging apparatus, cell plating apparatus, and cell evaluation apparatus; pharmaceutical apparatus and instruments, namely, incubators for cell and tissue cultures, parts and fittings for the aforesaid goods, cell culture dishes, cell culture flasks, cell culture multi-plates, cell culture inserts, cell analysis equipment, cell counting apparatus, cell sorting apparatus, cell feeding apparatus, cell passaging apparatus, cell plating apparatus, and cell evaluation apparatus; biotechnological laboratory apparatus and instruments, namely, incubators for cell and tissue cultures, parts and fittings for the aforesaid goods, cell culture dishes, cell culture flasks, cell culture multi-plates, cell culture inserts, cell analysis equipment, cell counting apparatus, cell sorting apparatus, cell feeding apparatus, cell passaging apparatus, cell plating apparatus, and cell evaluation apparatus; clinical diagnostic instruments, namely, incubators for cell and tissue cultures, parts and fittings for the aforesaid goods, cell culture dishes, cell culture flasks, cell culture multi-plates, cell culture inserts, cell analysis equipment, cell counting apparatus, cell sorting apparatus, cell feeding apparatus, cell passaging apparatus, cell plating apparatus, and cell evaluation apparatus; diagnostic apparatus for the detection of pathogens, genetic mutations, diseases or infections for laboratory or research use; regenerative medicine apparatus and instruments for laboratory use, namely, incubators for cell and tissue cultures, parts and fittings for the aforesaid goods, cell culture dishes, cell culture flasks, cell culture multi-plates, cell culture inserts, cell analysis equipment, cell counting apparatus, cell sorting apparatus, cell feeding apparatus, cell passaging apparatus, cell plating apparatus, and cell evaluation apparatus; temperature and climatic cabinets for life science, material science and drug discovery for industrial applications and laboratory purposes, in particular incubators for cell and tissue cultures; parts and fittings for the aforesaid goods; laboratory apparatus, namely, cell culture dishes, cell culture flasks, cell culture multi-plates, cell culture inserts, and cell culture beads for laboratory or medical use; laboratory apparatus, namely, cases for cell culture; laboratory incubators for culture and keeping of cells; laboratory apparatus and instruments for cell culture, namely, cell culture dishes, cell culture flasks, cell culture multi-plates, cell culture inserts and cell culture beads for laboratory or medical use; laboratory apparatus and instruments, namely, variable temperature incubators, variable temperature plates, variable humidity incubators, cell analysis equipment, cell counting apparatus, cell sorting apparatus, cell feeding apparatus, cell passaging apparatus, cell plating apparatus, and cell evaluation apparatus for laboratory or medical use
Aspects of the present invention relate to an automated cell culture instrument and to methods for operating such an instrument. In one aspect, the cell culture instrument includes a computing unit and a plurality of electrically controllable resources (e.g., sensors, actuators, etc.). The computing unit receives a protocol, schedules the resources so as to execute the protocol, and operates the resources as scheduled or in response to so as to provide an improved environment for cell culture growth. The computing unit may simulate protocol execution in connection with the scheduling or execution of the protocol.
G06F 15/173 - Communication entre processeurs utilisant un réseau d'interconnexion, p. ex. matriciel, de réarrangement, pyramidal, en étoile ou ramifié
In some aspects, the invention relates to selectively permeable cell culture vessel storage containers and their methods of use. In some embodiments, the containers comprise a gas-permeable membrane that is selectively impermeable to water vapor.
Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.
Aspects relate to an automated cell culture incubator and to methods for using such an incubator. In one aspect, the cell culture incubator includes an incubator cabinet having a transfer chamber and an internal chamber that are arranged to form an airlock configuration. In some embodiments, objects placed into the incubator cabinet are sterilized using ozone produced by an ozone generator. The incubator may include one or more transfer devices that move objects between the transfer chamber and the internal chamber.
In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.
Aspects relate to automated cell culture incubators comprising imaging systems, e.g., holographic or phase-contrast imagers, configured for imaging said cells within the incubators.
G06F 13/00 - Interconnexion ou transfert d'information ou d'autres signaux entre mémoires, dispositifs d'entrée/sortie ou unités de traitement
G06F 15/16 - Associations de plusieurs calculateurs numériques comportant chacun au moins une unité arithmétique, une unité programme et un registre, p. ex. pour le traitement simultané de plusieurs programmes
G06F 15/173 - Communication entre processeurs utilisant un réseau d'interconnexion, p. ex. matriciel, de réarrangement, pyramidal, en étoile ou ramifié
100.
CELL CULTURE INCUBATORS WITH INTEGRATED CELL MANIPULATION SYSTEMS
Aspects of the invention relate to automated cell culture incubators. In some embodiments, automated cell culture incubators comprise an integrated manipulation having one or more cell scrapers.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G02B 21/26 - PlatinesMoyens de réglage pour celles-ci
G03H 1/00 - Procédés ou appareils holographiques utilisant la lumière, les infrarouges ou les ultraviolets pour obtenir des hologrammes ou pour en obtenir une imageLeurs détails spécifiques
