Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
Disclosed herein includes systems, devices, and methods for determining a B-allele frequency (BAF) value (e.g., a normalized BAF value) of a SNP for a test sample obtained from a test subject (e.g., a human subject) from genome mapping (GM) data, such as optical genome mapping (OGM) data or electronic genome mapping (EGM) data.
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6825 - Détecteurs faisant intervenir la détection d’acides nucléiques
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Downloadable or recorded software in the field of genomic
bioinformatics for processing, storing, analyzing,
presenting, interpreting, and sharing genomic data; software
for genomic analysis used for medical diagnostic purposes,
or in scientific, clinical, or medical research; genomic
data analysis software. Software as a service (SAAS) or platform as a service (PAAS)
featuring software in the field of genomics for processing,
storing, analyzing, presenting, interpreting, and sharing
genomic data; product research and development and
consulting services for others in the fields of genomic
bioinformatics and data analysis; genomic data analysis
services for scientific, clinical, and medical research
purposes. Genomic data analysis services for medical diagnostic
purposes.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Downloadable or recorded software in the field of genomic bioinformatics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data; software for genomic analysis used for medical diagnostic purposes, or in scientific, clinical, or medical research; genomic data analysis software. (1) Software as a service (SAAS) or platform as a service (PAAS) featuring software in the field of genomics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data; product research and development and consulting services for others in the fields of genomic bioinformatics and data analysis; genomic data analysis services for scientific, clinical, and medical research purposes.
(2) Genomic data analysis services for medical diagnostic purposes.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
Top Hat illumination biological sample imaging devices are provided. Aspects of the imaging devices include: a laser illumination source configured to produce a collimated gaussian beam having a specific diameter; a beam converter configured to convert the collimated gaussian beam into a collimated, uniform irradiance over a region in space; a sample interrogation location in light receiving relationship with the beam converter; and a detector in light receiving relationship with a biological sample interrogation location. Also provided are methods of using the devices, e.g., in genome mapping and other applications.
G01N 21/84 - Systèmes spécialement adaptés à des applications particulières
G01N 21/63 - Systèmes dans lesquels le matériau analysé est excité de façon à ce qu'il émette de la lumière ou qu'il produise un changement de la longueur d'onde de la lumière incidente excité optiquement
Top Hat illumination biological sample imaging devices are provided. Aspects of the imaging devices include: a laser illumination source configured to produce a collimated gaussian beam having a specific diameter; a beam converter configured to convert the collimated gaussian beam into a collimated, uniform irradiance over a region in space; a sample interrogation location in light receiving relationship with the beam converter; and a detector in light receiving relationship with a biological sample interrogation location. Also provided are methods of using the devices, e.g., in genome mapping and other applications.
Disclosed herein include embodiments of a method of positioning (or leveling or moving) a sample (e.g., adjusting the tip-axis and the tilt axis of the sample or T nT motion stage). A sample can be provided. The sample can be in a sample chip or cartridge. In some embodiments, a method of positioning a sample can comprise: providing a sample. The method can comprise: placing the sample, or the sample chip or cartridge, on (or onto or into) a sample carrier of a TnT motion stage of a motion platform of the present disclosure. The TnT motion stage can be on an x-y motion stage of the motion platform.
Disclosed herein include a motion platform comprising a base; an x-y motion stage on the base; a tip-axis adjustment engagement component (e.g., a pawl) and/or a tilt-axis adjustment pawl engagement component (e.g., a pawl) on the base; a tip and tilt (TnT) motion stage on the x-y motion stage. The TnT motion stage can comprise: a sample carrier, two tip-axis goniometers with different slopes relative to one axis of the x-axis and the y-axis, and/or a tilt-axis slanted linear bearing. The TnT motion stage can comprise: a tip-axis complementary adjustment engagement component (e.g., a notch), and a tilt-axis complementary adjustment engagement component (e.g., a notch).
G02B 21/26 - PlatinesMoyens de réglage pour celles-ci
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
13.
REDUCTION OF BIAS IN GENOMIC COVERAGE MEASUREMENTS
Methods are provided for detecting and quantitating molecules using fluidics. In some embodiments, the methods comprise minimizing or eliminating biases caused by label density, or minimizing or eliminated biases caused by factors other than label density. In some embodiments, the methods comprise automated identification of genetic structural variation. In some embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
Disclosed herein includes systems, devices, and methods for determining a B-allele frequency (BAF) value (e.g., a normalized BAF value) of a SNP for a test sample obtained from a test subject (e.g., a human subject) from optical genome mapping (OGM) data.
G16B 25/10 - Profilage de l’expression de gènes ou de protéinesEstimation ou normalisation de ratio d’expression
G16B 20/20 - Détection d’allèles ou de variantes, p. ex. détection de polymorphisme d’un seul nucléotide
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 20/40 - Génétique de populationDéséquilibre de liaison
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
An imaging apparatus and software for genomic analysis in
scientific or medical research; an imaging system, namely,
an imaging apparatus and software sold as a unit, for
genomic analysis in scientific or medical research; software
for genomic analysis used for medical diagnostic purposes;
solid state substrates, namely, plates, chips, and wafers
for use in scientific or medical research, having a pattern
of channels for confining and linearizing biopolymer
samples; disposable substrates, namely, plates, chips, and
wafers for use in scientific or medical research, having a
pattern of channels to facilitate genomic imaging and
analysis; software for genomic analysis. A medical diagnostic imaging system, namely, an imaging
apparatus for genomic analysis; a medical imaging apparatus
for genomic analysis; solid state substrates, namely,
plates, chips, and wafers for use in clinical medicine,
having a pattern of channels for confining and linearizing
biopolymer samples; disposable substrates, namely, plates,
chips, and wafers for use in clinical medicine, having a
pattern of channels to facilitate genomic imaging and
analysis.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Reagents for use in preparing molecules for genomic research
and analysis; a kit for extracting and labeling biopolymers
for research purposes, containing enzymes, color reagents,
buffers, and other reagents; polynucleotide isolation and
purification kit consisting of enzymes, color reagents,
buffers, and other reagents used for scientific or medical
research purposes; kits for extracting and labeling
polynucleotide molecules for research purposes, containing
enzymes, color reagents, buffers, and other reagents;
reagents for use in preparing polynucleotide molecules for
genomic research; diagnostic kits containing enzymes, color
reagents, buffers, and other reagents for extracting and
labeling polynucleotide molecules. Reagents for use in clinical medicine for preparing
molecules for genomic analysis; a medical diagnostic kit
containing enzymes, color reagents, buffers, and other
reagents for extracting and labeling biopolymers. An imaging system, namely, an imaging apparatus and
software, for the analysis of biopolymer samples in
scientific or medical research; software used in the
analysis of biopolymer samples for medical diagnostic
purposes; software used in the analysis of biopolymer
samples for scientific or medical research; an imaging
apparatus for use in the analysis of biopolymer samples in
scientific or medical research; nanofluidic devices, namely,
solid state substrates in the nature of plates, chips and
wafers, and software, both of the foregoing for genomic
imaging and analysis; an imaging apparatus and software for
genomic analysis in scientific or medical research; an
imaging system, namely, an imaging apparatus and software
sold as a unit, for genomic analysis in scientific or
medical research; software for genomic analysis used for
medical diagnostic purposes, or in scientific, clinical or
medical research; genomic data analysis software; software
for interpreting and analyzing data generated by an imaging
apparatus for the purpose of studying biopolymer samples;
solid state substrates, namely, plates, chips, and wafers
for use in scientific or medical research, having a pattern
of channels for confining and linearizing biopolymer
samples; disposable substrates, namely, plates, chips, and
wafers for use in scientific or medical research, having a
pattern of channels to facilitate genomic imaging and
analysis; solid state substrates, namely, plates, chips and
wafers having a plurality of fluid passageways for confining
and linearizing macromolecules including biopolymer
molecules for scientific or medical research; nanochannel
wafers, for scientific or medical research, used in
instruments for genomic analysis; downloadable or recorded
software in the field of genomic bioinformatics for
processing, storing, analyzing, presenting, interpreting,
and sharing genomic data. A medical diagnostic imaging system, namely, an imaging
apparatus and software sold as a unit and used in the
analysis of biopolymer samples; a medical diagnostic imaging
apparatus used in the analysis of biopolymer samples; a
medical diagnostic imaging system, namely, an imaging
apparatus and software sold as a unit for genomic analysis;
a medical imaging apparatus for genomic analysis; solid
state substrates, namely, plates, chips, and wafers for use
in clinical medicine, having a pattern of channels for
confining and linearizing biopolymer samples; disposable
substrates, namely, plates, chips, and wafers for use in
clinical medicine, having a pattern of channels to
facilitate genomic imaging and analysis; medical diagnostic
solid state substrates, namely, plates, chips and wafers for
confining and linearizing macromolecules including
biopolymer molecules; nanochannel wafers, for use in
clinical medicine, used in instruments for genomic analysis;
medical specimen collection and transportation kit comprised
of blood collection tubes, foam container for tubes,
absorbent pad, biohazard bag, medical indicator marker and
labels, for molecular and genetic testing for disorders of
childhood development. Product research and development and consulting services for
others in the fields of biopolymer imaging and analysis;
genomic mapping, genomic imaging, and genomic analysis
services for scientific research purposes; medical,
pharmaceutical, biotechnology, and scientific research and
consulting services in the field of genetics, namely,
analysis of relationships between genetic variants and
neurodevelopmental disorders, genetic research, bio-marker
discovery research, and pheno-typing for identifying,
testing and developing diagnostics and treatments for human
diseases and human disorders; software as a service (SAAS)
or platform as a service (PAAS) featuring software in the
field of genomics for processing, storing, analyzing,
presenting, interpreting, and sharing genomic data; product
research and development and consulting services for others
in the fields of genomic bioinformatics and data analysis;
genomic data analysis services for scientific, clinical, and
medical research purposes. Genomic mapping, genomic imaging, genomic analysis, and
genomic data analysis services for medical diagnostic
purposes; providing medical analysis services in the nature
of relationships between genetic variants and
neurodevelopmental disorders for identifying, testing, and
developing diagnostic tools and treatments for such
disorders; medical testing services, namely, molecular and
genetic testing for disorders of childhood development;
providing information related to medical testing services
via an internet website for medical professionals and
medical patients; providing molecular and genetic medical
testing results via the internet.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Solid state substrates, namely, plates, chips, and wafers
for use in scientific or medical research, having a pattern
of channels for confining and linearizing biopolymer
samples; disposable substrates, namely, plates, chips, and
wafers for use in scientific or medical research, having a
pattern of channels to facilitate genomic imaging and
analysis. Solid state substrates, namely, plates, chips, and wafers
for use in clinical medicine, having a pattern of channels
for confining and linearizing biopolymer samples; disposable
substrates, namely, plates, chips, and wafers for use in
clinical medicine, having a pattern of channels to
facilitate genomic imaging and analysis.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Reagents for use in preparing molecules for genomic research and analysis; a kit for extracting and labeling biopolymers for research purposes, containing enzymes, color reagents, buffers, and other reagents; polynucleotide isolation and purification kit consisting of enzymes, color reagents, buffers, and other reagents used for scientific or medical research purposes; kits for extracting and labeling polynucleotide molecules for research purposes, containing enzymes, color reagents, buffers, and other reagents; reagents for use in preparing polynucleotide molecules for genomic research; diagnostic kits containing enzymes, color reagents, buffers, and other reagents for extracting and labeling polynucleotide molecules.
(2) Reagents for use in clinical medicine for preparing molecules for genomic analysis; a medical diagnostic kit containing enzymes, color reagents, buffers, and other reagents for extracting and labeling biopolymers.
(3) An imaging system, namely, an imaging apparatus and software, for the analysis of biopolymer samples in scientific or medical research; software used in the analysis of biopolymer samples for medical diagnostic purposes; software used in the analysis of biopolymer samples for scientific or medical research; an imaging apparatus for use in the analysis of biopolymer samples in scientific or medical research; nanofluidic devices, namely, solid state substrates in the nature of plates, chips and wafers, and software, both of the foregoing for genomic imaging and analysis; an imaging apparatus and software for genomic analysis in scientific or medical research; an imaging system, namely, an imaging apparatus and software sold as a unit, for genomic analysis in scientific or medical research; software for genomic analysis used for medical diagnostic purposes, or in scientific, clinical or medical research; genomic data analysis software; software for interpreting and analyzing data generated by an imaging apparatus for the purpose of studying biopolymer samples; solid state substrates, namely, plates, chips, and wafers for use in scientific or medical research, having a pattern of channels for confining and linearizing biopolymer samples; disposable substrates, namely, plates, chips, and wafers for use in scientific or medical research, having a pattern of channels to facilitate genomic imaging and analysis; solid state substrates, namely, plates, chips and wafers having a plurality of fluid passageways for confining and linearizing macromolecules including biopolymer molecules for scientific or medical research; nanochannel wafers, for scientific or medical research, used in instruments for genomic analysis; downloadable or recorded software in the field of genomic bioinformatics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data.
(4) A medical diagnostic imaging system, namely, an imaging apparatus and software sold as a unit and used in the analysis of biopolymer samples; a medical diagnostic imaging apparatus used in the analysis of biopolymer samples; a medical diagnostic imaging system, namely, an imaging apparatus and software sold as a unit for genomic analysis; a medical imaging apparatus for genomic analysis; solid state substrates, namely, plates, chips, and wafers for use in clinical medicine, having a pattern of channels for confining and linearizing biopolymer samples; disposable substrates, namely, plates, chips, and wafers for use in clinical medicine, having a pattern of channels to facilitate genomic imaging and analysis; medical diagnostic solid state substrates, namely, plates, chips and wafers for confining and linearizing macromolecules including biopolymer molecules; nanochannel wafers, for use in clinical medicine, used in instruments for genomic analysis; medical specimen collection and transportation kit comprised of blood collection tubes, foam container for tubes, absorbent pad, biohazard bag, medical indicator marker and labels, for molecular and genetic testing for disorders of childhood development. (1) Product research and development and consulting services for others in the fields of biopolymer imaging and analysis; genomic mapping, genomic imaging, and genomic analysis services for scientific research purposes; medical, pharmaceutical, biotechnology, and scientific research and consulting services in the field of genetics, namely, analysis of relationships between genetic variants and neurodevelopmental disorders, genetic research, bio-marker discovery research, and pheno-typing for identifying, testing and developing diagnostics and treatments for human diseases and human disorders; software as a service (SAAS) or platform as a service (PAAS) featuring software in the field of genomics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data; product research and development and consulting services for others in the fields of genomic bioinformatics and data analysis; genomic data analysis services for scientific, clinical, and medical research purposes.
(2) Genomic mapping, genomic imaging, genomic analysis, and genomic data analysis services for medical diagnostic purposes; providing medical analysis services in the nature of relationships between genetic variants and neurodevelopmental disorders for identifying, testing, and developing diagnostic tools and treatments for such disorders; medical testing services, namely, molecular and genetic testing for disorders of childhood development; providing information related to medical testing services via an internet website for medical professionals and medical patients; providing molecular and genetic medical testing results via the internet.
19.
NANONOZZLE DEVICE ARRAYS: THEIR PREPARATION AND USE FOR MACROMOLECULAR ANALYSIS
Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
10 - Appareils et instruments médicaux
Produits et services
Medical testing services, namely, genomic mapping, genomic imaging, and genomic analysis services for medical diagnostic purposes; providing an internet website for medical professionals and medical patients featuring information in the field of medical testing services for diagnostic or treatment purposes; providing medical information, namely, reporting of molecular and genetic medical testing results to medical patients and medical professionals via the internet Medical specimen collection and transportation kit comprised of collection tubes, foam container for tubes, absorbent pad, biohazard bag, marker, labels, mail packaging, forms and instructions, all for testing of patient samples for medical and/or diagnostic purposes
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Downloadable or recorded software in the field of genomic
bioinformatics for processing, storing, analyzing,
presenting, interpreting, and sharing genomic data; software
for genomic analysis used for medical diagnostic purposes,
or in scientific, clinical, or medical research; genomic
data analysis software. Software as a service (SAAS) or platform as a service (PAAS)
featuring software in the field of genomics for processing,
storing, analyzing, presenting, interpreting, and sharing
genomic data; product research and development and
consulting services for others in the fields of genomic
bioinformatics and data analysis; genomic data analysis
services for scientific, clinical, and medical research
purposes. Genomic data analysis services for medical diagnostic
purposes.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Downloadable or recorded software in the field of genomic
bioinformatics for processing, storing, analyzing,
presenting, interpreting, and sharing genomic data; software
for genomic analysis used for medical diagnostic purposes,
or in scientific, clinical, or medical research; genomic
data analysis software. Software as a service (SAAS) or platform as a service (PAAS)
featuring software in the field of genomics for processing,
storing, analyzing, presenting, interpreting, and sharing
genomic data; product research and development and
consulting services for others in the fields of genomic
bioinformatics and data analysis; genomic data analysis
services for scientific, clinical, and medical research
purposes. Genomic data analysis services for medical diagnostic
purposes.
23.
Integrated analysis devices and related fabrication methods and analysis techniques
Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
09 - Appareils et instruments scientifiques et électriques
Produits et services
An imaging apparatus and recorded software for genomic analysis in scientific or medical research; An imaging system, namely, an imaging apparatus and recorded software sold as a unit, for genomic analysis in scientific or medical research; downloadable and recorded software for genomic analysis used for medical diagnostic purposes; Solid state substrates, namely, plates, chips, and wafers for use in scientific or medical research, having a pattern of channels for confining and linearizing biopolymer samples; Disposable substrates, namely, plates, chips, and wafers for use in scientific or medical research, having a pattern of channels to facilitate genomic imaging and analysis
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for use in preparing molecules for genomic research and analysis; a kit for extracting and labeling biopolymers for research purposes, containing enzymes, color reagents, buffers, and other reagents; Polynucleotide isolation and purification kit consisting of enzymes, color reagents, buffers, and other reagents used for scientific or medical research purposes; kits for extracting and labeling polynucleotide molecules for research purposes, containing enzymes, color reagents, buffers, and other reagents; reagents for use in preparing polynucleotide molecules for genomic research; diagnostic kits containing enzymes, color reagents, buffers, and other reagents for extracting and labeling polynucleotide molecules An imaging system, namely, an imaging apparatus and downloadable and recorded software, for the analysis of biopolymer samples in scientific or medical research; downloadable and recorded software used in the analysis of biopolymer samples for medical diagnostic purposes; downloadable and recorded software used in the analysis of biopolymer samples for scientific or medical research; an imaging apparatus for use in the analysis of biopolymer samples in scientific or medical research; Nanofluidic devices, namely, solid state substrates in the nature of plates, chips and wafers, and downloadable and recorded software, both of the foregoing for genomic imaging and analysis; An imaging apparatus and downloadable and recorded software for genomic analysis in scientific or medical research; an imaging system, namely, an imaging apparatus and downloadable and recorded software sold as a unit, for genomic analysis in scientific or medical research; downloadable and recorded software for genomic analysis used for medical diagnostic purposes, and in scientific, clinical, and medical research; downloadable and recorded genomic data analysis software; downloadable and recorded software for interpreting and analyzing data generated by an imaging apparatus for the purpose of studying biopolymer samples; Solid state substrates, namely, plates, chips, and wafers for use in scientific or medical research, having a pattern of channels for confining and linearizing biopolymer samples; disposable substrates, namely, plates, chips, and wafers for use in scientific or medical research, having a pattern of channels to facilitate genomic imaging and analysis; Solid state substrates, namely, plates, chips and wafers having a plurality of fluid passageways for confining and linearizing macromolecules including biopolymer molecules for scientific or medical research; nanochannel wafers, for scientific or medical research, used in instruments for genomic analysis; downloadable and recorded software in the field of genomic bioinformatics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data product research and development and consulting services for others in the fields of biopolymer imaging and analysis; genomic mapping, genomic imaging, and genomic analysis services for scientific research purposes; medical, pharmaceutical, biotechnology, and scientific research and consulting services in the field of genetics, namely, analysis of relationships between genetic variants and neurodevelopmental disorders, genetic research, bio-marker discovery research, and pheno-typing for identifying, testing and developing diagnostics and treatments for human diseases and human disorders; software as a service (SAAS) services and platform as a service (PAAS) featuring software and computer software platforms featuring software in the field of genomics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data; product research and development and consulting services for others in the fields of genomic bioinformatics and data analysis; genomic data analysis services for scientific, clinical, and medical research purposes
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 30/10 - Alignement de séquenceRecherche d’homologie
09 - Appareils et instruments scientifiques et électriques
Produits et services
downloadable and recorded software in the field of genomic bioinformatics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data; downloadable and recorded software for genomic analysis used for medical diagnostic purposes, and in scientific, clinical, or medical research; downloadable and recorded genomic data analysis software
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
software as a service (SAAS) services and platform as a service (PAAS) featuring software and computer software platforms in the field of genomics for processing, storing, analyzing, presenting, interpreting, and sharing genomic data; product research and development and consulting services for others in the fields of genomic bioinformatics and data analysis; genomic data analysis services for scientific, clinical, and medical research purposes
29.
PHOTOCLEAVAGE METHOD AND APPARATUS TO CLEAN FLUIDIC DEVICES
A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B08B 7/00 - Nettoyage par des procédés non prévus dans une seule autre sous-classe ou un seul groupe de la présente sous-classe
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Medical specimen collection and transportation kit comprised
of blood collection tubes, foam container for tubes,
absorbent pad, biohazard bag, and medical indicator marker
and labels, for molecular and genetic testing for disorders
of childhood development. Medical testing services, namely, molecular and genetic
testing for disorders of childhood development; providing
information related to medical testing services via an
internet website for medical professionals and medical
patients; providing molecular and genetic medical testing
results via the internet; genomic mapping, genomic imaging,
and genomic analysis services for medical diagnostic
purposes.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Medical specimen collection and transportation kit comprised
of blood collection tubes, foam container for tubes,
absorbent pad, biohazard bag, marker, labels, mail
packaging, forms and instructions, for molecular and genetic
testing for disorders of childhood development. Medical testing services, namely, molecular and genetic
testing for disorders of childhood development; providing an
internet website for medical professionals and medical
patients featuring information related to medical testing
services; providing molecular and genetic medical testing
results via the internet; genomic mapping, genomic imaging,
and genomic analysis services for medical diagnostic
purposes.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Medical specimen collection and transportation kit comprised of blood collection tubes, foam container for tubes, absorbent pad, biohazard bag, and medical indicator marker and labels, for molecular and genetic testing for disorders of childhood development. (1) Medical testing services, namely, molecular and genetic testing for disorders of childhood development; providing information related to medical testing services via an internet website for medical professionals and medical patients; providing molecular and genetic medical testing results via the internet; genomic mapping, genomic imaging, and genomic analysis services for medical diagnostic purposes.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Medical specimen collection and transportation kit comprised of blood collection tubes, foam container for tubes, absorbent pad, biohazard bag, marker, labels, mail packaging, forms and instructions, for molecular and genetic testing for disorders of childhood development. (1) Medical testing services, namely, molecular and genetic testing for disorders of childhood development; providing an internet website for medical professionals and medical patients featuring information related to medical testing services; providing molecular and genetic medical testing results via the internet; genomic mapping, genomic imaging, and genomic analysis services for medical diagnostic purposes.
34.
Methods and devices for single-molecule whole genome analysis
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6825 - Détecteurs faisant intervenir la détection d’acides nucléiques
Methods of labeling DNA molecules comprising the use of one or more dCas proteins and one or more labeled guide RNAs (gRNAs) are described herein. In some embodiments, the labeled DNA molecules are elongated in fluidic nanochannels, where they can be analyzed.
Methods are provided for detecting and quantitating molecules using fluidics. In some embodiments, the methods comprise minimizing or eliminating biases caused by label density, or minimizing or eliminated biases caused by factors other than label density. In some embodiments, the methods comprise automated identification of genetic structural variation. In some embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
B82Y 30/00 - Nanotechnologie pour matériaux ou science des surfaces, p. ex. nanocomposites
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
39.
Methods of determining nucleic acid structural information
Methods of double-stranded nucleic acid sequence determination and assembly that are able to identify insertions, deletions, repeat region sizes and genomic rearrangements, for example, are disclosed herein, which can use relatively large labeled nucleic acid fragments to analyze the structure of even larger genetic regions. In some embodiments these methods involve the use of certain parameters which unexpectedly improve overall method performance. In some embodiments these methods involve sample labeling that does not result in the formation of single-stranded nucleic acid fragment labeling intermediaries.
Methods are provided for detecting and quantitating molecules using fluidics. In preferred embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G16B 30/10 - Alignement de séquenceRecherche d’homologie
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
41.
Methods and devices for single-molecule whole genome analysis
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
09 - Appareils et instruments scientifiques et électriques
Produits et services
Genomic data analysis software; software for interpreting
and analyzing data generated by an imaging apparatus for the
purpose of studying biopolymer samples.
Methods of labeling DNA molecules comprising the use of one or more dCas proteins and one or more labeled guide RNAs (gRNAs) are described herein. In some embodiments, the labeled DNA molecules are elongated in fluidic nanochannels, where they can be analyzed.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Nucleic acid extraction and purification system, for scientific research purposes, the aforementioned system comprised of laboratory equipment, namely, a laboratory apparatus that utilizes isotachophoresis to extract, purify, isolate and concentrate nucleic acid from biological samples incorporating a touchscreen, downloadable software, and nucleic acid fluidics chips
45.
Nanonozzle device arrays: their preparation and use for macromolecular analysis
Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Polynucleotide isolation, purification, and labeling kit
including enzymes, color reagents, buffers, and other
reagents or combinations thereof, used for scientific or
medical research purposes; kits for extracting and labeling
polynucleotide molecules for research purposes, containing
enzymes, color reagents, buffers, and other reagents, or
combinations thereof; reagents for use in preparing
polynucleotide molecules for genomic research; diagnostic
kits containing enzymes, color reagents, buffers, and other
reagents, or combinations thereof, for extracting and
labeling polynucleotide molecules. Reagents for use in clinical medicine for preparing
polynucleotide molecules for genomic analysis. Reaction preparation device and slides for polynucleotide
isolation, purification and labeling; reaction preparation
device and slides for extracting and labeling polynucleotide
molecules for research purposes; reaction preparation device
and slides for use in preparing polynucleotide molecules for
genomic research; reaction preparation device and slides for
extracting and labeling polynucleotide molecules for
scientific research purposes; reaction preparation device
and slides for use in clinical medicine research for
preparing polynucleotide molecules for genomic analysis.
48.
Integrated analysis devices and related fabrication methods and analysis techniques
Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B08B 7/00 - Nettoyage par des procédés non prévus dans une seule autre sous-classe ou un seul groupe de la présente sous-classe
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Solid state substrates, namely, plates, chips, and wafers
for use in scientific or medical research, having a pattern
of channels for confining and linearizing biopolymer
samples; disposable substrates, namely, plates, chips, and
wafers for use in scientific or medical research, having a
pattern of channels to facilitate genomic imaging and
analysis. Solid state substrates, namely, plates, chips, and wafers
for use in clinical medicine, having a pattern of channels
for confining and linearizing biopolymer samples; disposable
substrates, namely, plates, chips, and wafers for use in
clinical medicine, having a pattern of channels to
facilitate genomic imaging and analysis.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
An imaging apparatus and software for genomic analysis in
scientific or medical research; an imaging system, namely,
an imaging apparatus and software sold as a unit, for
genomic analysis in scientific or medical research; software
for genomic analysis used for medical diagnostic purposes. A medical diagnostic imaging system, namely, an imaging
apparatus and software sold as a unit for genomic analysis;
a medical imaging apparatus for genomic analysis.
52.
Methods and devices for single-molecule whole genome analysis
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
09 - Appareils et instruments scientifiques et électriques
Produits et services
genomic data analysis software; software for interpreting and analyzing data generated by an imaging apparatus for the purpose of studying biopolymer samples
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
SOLID STATE SUBSTRATES, NAMELY, PLATES, CHIPS, AND WAFERS FOR USE IN SCIENTIFIC OR MEDICAL RESEARCH, HAVING A PATTERN OF CHANNELS FOR CONFINING AND LINEARIZING BIOPOLYMER SAMPLES; DISPOSABLE SUBSTRATES, NAMELY, PLATES, CHIPS, AND WAFERS FOR USE IN SCIENTIFIC OR MEDICAL RESEARCH, HAVING A PATTERN OF CHANNELS TO FACILITATE GENOMIC IMAGING AND ANALYSIS SOLID STATE SUBSTRATES, NAMELY, PLATES, CHIPS, AND WAFERS FOR USE IN CLINICAL MEDICINE, HAVING A PATTERN OF CHANNELS FOR CONFINING AND LINEARIZING BIOPOLYMER SAMPLES; DISPOSABLE SUBSTRATES, NAMELY, PLATES, CHIPS, AND WAFERS FOR USE IN CLINICAL MEDICINE, HAVING A PATTERN OF CHANNELS TO FACILITATE GENOMIC IMAGING AND ANALYSIS
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
AN IMAGING APPARATUS AND SOFTWARE FOR GENOMIC ANALYSIS IN SCIENTIFIC OR MEDICAL RESEARCH; AN IMAGING SYSTEM, NAMELY, AN IMAGING APPARATUS AND SOFTWARE SOLD AS A UNIT, FOR GENOMIC ANALYSIS IN SCIENTIFIC OR MEDICAL RESEARCH; SOFTWARE FOR GENOMIC ANALYSIS USED FOR MEDICAL DIAGNOSTIC PURPOSES A MEDICAL DIAGNOSTIC IMAGING SYSTEM, NAMELY, AN IMAGING APPARATUS AND SOFTWARE SOLD AS A UNIT FOR GENOMIC ANALYSIS; A MEDICAL IMAGING APPARATUS FOR GENOMIC ANALYSIS
56.
Methods of determining nucleic acid structural information
Methods of double-stranded nucleic acid sequence determination and assembly that are able to identify insertions, deletions, repeat region sizes and genomic rearrangements, for example, are disclosed herein, which can use relatively large labeled nucleic acid fragments to analyze the structure of even larger genetic regions. In some embodiments these methods involve the use of certain parameters which unexpectedly improve overall method performance. In some embodiments these methods involve sample labeling that does not result in the formation of single-stranded nucleic acid fragment labeling intermediaries.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
Methods are provided for detecting and quantitating molecules using fluidics. In some embodiments, the methods comprise minimizing or eliminating biases caused by label density, or minimizing or eliminated biases caused by factors other than label density. In some embodiments, the methods comprise automated identification of genetic structural variation. In some embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
58.
Methods of macromolecular analysis using nanochannel arrays
Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as wen as the devices for carrying out such high throughput analysis in a massively parallel fashion. Methods of fabricating such devices are also disclosed.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
According to some embodiments herein, methods and kits for labeling and analyzing nucleic acids are provided. In some embodiments, sequence-specific labeling is performed on polynucleotide sequences associated with a host genome, and the presence or absence of patterns characteristic of extragenomic sequences are determined.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
C12Q 1/6893 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les protozoaires
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 30/10 - Alignement de séquenceRecherche d’homologie
The present invention provides methods of obtaining structural information about a biopolymer sample. The methods include labeling portions of a biopolymer, such as DNA or RNA, linearizing the biopolymer in some cases, and determining the distance between the labels. The user can then compare different samples' between-label distances to qualitatively compare different samples and to assay a given sample for additions or deletions of nucleotides in the regions flanked by the labels. The methods also permit sequencing of biopolymers.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
61.
PHOTOCLEAVAGE METHOD AND APPARATUS TO CLEAN FLUIDIC DEVICES
A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B08B 7/00 - Nettoyage par des procédés non prévus dans une seule autre sous-classe ou un seul groupe de la présente sous-classe
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
Methods are provided for characterizing a sample comprising polynucleotide sequences. The methods can comprise labeling nucleic acid molecules of the sample, translocating the plurality of labeled sample nucleic acid molecules though one or more fluidic nanochannels, detecting physical counts of signals from the labeled sample nucleic acid molecule, and determining a copy number of a genome or a fragment or fragments thereof in the sample.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C40B 40/06 - Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G06F 19/22 - pour la comparaison de séquences impliquant des nucléotides ou des acides aminés, p.ex. recherche d'homologie, identification de motifs ou de polymorphismes de nucléotides simples [SNP] ou alignement de séquences
Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples.
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
64.
Nanonozzle device arrays: their preparation and use for macromolecular analysis
Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
B82Y 30/00 - Nanotechnologie pour matériaux ou science des surfaces, p. ex. nanocomposites
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
Methods and compositions for processing polynucleotides are provided according to some embodiments herein. In some embodiments, a sample is immobilized in a porous matrix, and non-polynucleotides are removed from the sample. In some embodiments, polynucleotides are labeled or enzymatically modified the matrix. In some embodiments, labeled or enzymatically modified polynucleotides are removed from the matrix for analysis.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
66.
REDUCTION OF BIAS IN GENOMIC COVERAGE MEASUREMENTS
Methods are provided for detecting and quantitating molecules using fluidics. In some embodiments, the methods comprise minimizing or eliminating biases caused by label density, or minimizing or eliminated biases caused by factors other than label density. In some embodiments, the methods comprise automated identification of genetic structural variation. In some embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
67.
IMPROVED METHODS OF DETERMINING NUCLEIC ACID STRUCTURAL INFORMATION
Methods of double-stranded nucleic acid sequence determination and assembly that are able to identify insertions, deletions, repeat region sizes and genomic rearrangements, for example, are disclosed herein, which can use relatively large labeled nucleic acid fragments to analyze the structure of even larger genetic regions. In some embodiments these methods involve the use of certain parameters which unexpectedly improve overall method performance. In some embodiments these methods involve sample labeling that does not result in the formation of single-stranded nucleic acid fragment labeling intermediaries.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
Disclosed herein are cartridges and systems for nanoanalysis, and methods for using the cartridges and systems. For example, a cartridge can have a chip forming one or more sample wells, with a plurality of nanotubes running between a pair of adjacent sample wells, the nanotubes configured to receive linearized molecules. Upon linearization, the molecules in the nanochannels are imaged through a transparent substrate on which the chip is located. The movement of molecules through the nanochannels can be started and stopped, and cartridge can be moved to facilitate imaging of the molecules.
According to some embodiments herein, methods and kits for labeling and analyzing nucleic acids are provided, In some embodiments, sequence-specific labeling is performed on polynucleotide sequences associated with a host genome, and the presence or absence of patterns characteristic of extragenomic sequences are determined.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
70.
Methods of macromolecular analysis using nanochannel arrays
Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as wen as the devices for carrying out such high throughput analysis in a massively parallel fashion. Methods of fabricating such devices are also disclosed.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
Methods are provided for detecting and quantitating molecules using fluidics. In preferred embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
73.
Methods and devices for single-molecule whole genome analysis
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
G06F 19/18 - pour la génomique ou la protéomique fonctionnelle, p.ex. associations génotype-phénotype, déséquilibre de liaison, mutagénèse, génotypage ou annotation génomique, interactions protéines-protéines ou interactions protéines-acides nucléiques
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for use in preparing molecules for genomic research and analysis; a kit for extracting and labeling biopolymers for research purposes, containing enzymes, color reagents, buffers, and other reagents Reagents for use in clinical medicine for preparing molecules for genomic analysis; a medical diagnostic kit containing enzymes, color reagents, buffers, and other reagents for extracting and labeling biopolymers An imaging system, namely, an imaging apparatus and software, for the analysis of biopolymer samples in scientific or medical research; software used in the analysis of biopolymer samples for medical diagnostic purposes; software used in the analysis of biopolymer samples for scientific or medical research; an imaging apparatus for use in the analysis of biopolymer samples in scientific or medical research A medical diagnostic imaging system, namely, an imaging apparatus and software sold as a unit and used in the analysis of biopolymer samples; a medical diagnostic imaging apparatus used in the analysis of biopolymer samples Product research and development and consulting services for others in the fields of biopolymer imaging and analysis; genomic mapping, genomic imaging, and genomic analysis services for scientific research purposes
75.
PHYSICAL MAP CONSTRUCTION OF WHOLE GENOME AND POOLED CLONE MAPPING IN NANOCHANNEL ARRAY
Methods for generating physical maps for polynucleotides, such as genomic DNA, are disclosed herein. Also disclosed are methods for identifying the source of polynucleotides. The methods can, for example, be used in physical map construction of whole genome. In addition, methods and systems capable of performing high throughput characterization of macromolecules using nanofludic devices are enclosed.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
09 - Appareils et instruments scientifiques et électriques
Produits et services
An imaging system, imaging apparatus, or software for use in
the analysis of polymer samples in scientific or medical
research; an imaging system, namely, an imaging apparatus
and software, for genomic analysis in scientific or medical
research.
Provided are devices and methods for polymer analysis, in which labeled polymers are translocated along nanochannels that have illuminated detection regions within. As a labeled polymer translocates along the nanochannel and the labels pass over the illuminated detection regions, the labels become detectable (e.g., where different labels fluoresce in response to different wavelengths present at different illumination regions), and the user generates a spatial map of the location of the various labels along the length of the polymer. The location of the labels is then correlated to one or more structural characteristics of the polymer.
G01N 33/558 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant la diffusion ou la migration de l'anticorps ou de l'antigène
B82Y 30/00 - Nanotechnologie pour matériaux ou science des surfaces, p. ex. nanocomposites
09 - Appareils et instruments scientifiques et électriques
Produits et services
Nanofluidic devices, namely, solid state substrates in the nature of plates, chips and wafers, and software, both of the foregoing for genomic imaging and analysis
80.
SYSTEMS AND METHODS FOR ASSESSING BIOMOLECULE CHARACTERISTICS
Provided are methods and systems for assessing the presence and extent of damage on a polynucleotide. The methods include incorporating a label at the site of the damage and imaging the label to determine the presence and extent of the damage. The systems include devices capable of performing damage assessment on single molecules.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
The present invention provides methods of obtaining structural information about a biopolymer sample. The methods include labeling portions of a biopolymer, such as DNA or RNA, linearizing the biopolymer in some cases, and determining the distance between the labels. The user can then compare different samples' between-label distances to qualitatively compare different samples and to assay a given sample for additions or deletions of nucleotides in the regions flanked by the labels. The methods also permit sequencing of biopolymers.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
82.
Integrated analysis devices and related fabrication methods and analysis techniques
Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determining structural variations and copy number variations between individuals.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
84.
METHODS AND RELATED DEVICES FOR SINGLE MOLECULE WHOLE GENOME ANALYSIS
Provided are methods of labeling and analyzing features along at least one macro molecule such as a linear biopolymer, including methods of mapping the distribution and frequency of specific sequence motifs or the chemical or proteomic modification state of such sequence motifs along individual unfolded nucleic acid molecules. The present invention also provides methods of identifying signature patterns of sequence or epigenetic variations along such labeled macro molecules for direct massive parallel single molecule level analysis. The present invention also provides systems suitable for high throughput analysis of such labeled macro mo lecules.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
85.
NANOCHANNEL ARRAYS AND NEAR-FIELD ILLUMINATION DEVICES FOR POLYMER ANALYSIS AND RELATED METHODS
Provided are devices and methods for polymer analysis, in which labeled polymers are translocated along nanochannels that have illuminated detection regions within. As a labeled polymer translocates along the nanochannel and the labels pass over the illuminated detection regions, the labels become detectable (e.g., where different labels fluoresce in response to different wavelengths present at different illumination regions), and the user generates a spatial map of the location of the various labels along the length of the polymer. The location of the labels is then correlated to one or more structural characteristics of the polymer.
C40B 60/12 - Appareils spécialement adaptés à une utilisation en chimie combinatoire ou avec des bibliothèques pour cribler des bibliothèques
C40B 30/04 - Procédés de criblage des bibliothèques en mesurant l'aptitude spécifique à se lier à une molécule cible, p. ex. liaison anticorps-antigène, liaison récepteur-ligand
86.
DEVICES AND METHODS FOR DYNAMIC DETERMINATION OF SAMPLE SPATIAL ORIENTATION AND DYNAMIC REPOSITIONING
Provided are devices and methods for determining the spatial orientation of a target sample, which devices and methods are useful in auto focus systems. The devices and methods function by correlating (a) the location of radiation on a radiation detector of radiation reflected by the sample with (b) the position of the sample, and in some embodiments, adjusting the position of the sample, the position of an optical device, or both, in accordance with the location of radiation reflected by the sample onto the detector so as to maintain the sample in focus.
Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 15/06 - Recherche de la concentration des suspensions de particules
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
B82Y 30/00 - Nanotechnologie pour matériaux ou science des surfaces, p. ex. nanocomposites
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
88.
Methods of macromolecular analysis using nanochannel arrays
Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as well as the devices for carrying out such high throughput analysis in a massively parallel fashion. Methods of fabricating such devices are also disclosed.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
89.
NANONOZZLE DEVICE ARRAYS: THEIR PREPARATION AND USE FOR MACROMOLECULAR ANALYSIS
Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.
Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as well as the devices for carrying out such high throughput analysis in a massively parallel fashion. Methods of fabricating such devices are also disclosed. The sample embodiment shown in the figure illustrates detection of a macromolecule flowing through a nanochannel device where passage of the macromolecule through the nanochannel is recorded by exciting features of interest to fluoresce with an excitation source, and then sensing the fluorescence with a photon detection device, the resulting fluorescent signal being correlated along the length of the macromolecule.
Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have re-duced background signals and that reduce quench-ing of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples.
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
Abstract Provided herein, in some embodiments, are devices that address the need for efficient determination of the sizes and composition of DNA or other macromolecules by linearizing and analyzing such molecules. Devices for analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules are disclosed. In some embodiments, the devices can perform such high throughput analysis in a massively parallel fashion. In some embodiments, the devices disclosed herein advantageously enable a minimal amount of sample to be used. Date Recue/Date Received 2020-06-15
B82Y 35/00 - Procédés ou appareils pour la mesure ou l’analyse des nanostructures
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
94.
METHODS AND DEVICES FOR SINGLE-MOLECULE WHOLE GENOME ANALYSIS
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determining structural variations and copy number variations between individuals.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entailprocessing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determiningstructural variations and copy number variations between individuals.
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
The present invention provides methods of obtaining structural information about a biopolymer sample. The meth-ods include labeling portions of a biopolymer, such as DNA or RNA, linearizing the biopolymer in some cases, and determining the distance between the labels. The user can then compare different samples' between-label distances to qualitatively compare different samples and to assay a given sample for additions or deletions of nucleotides in the regions flanked by the labels. The methods also permit sequencing of biopolymers.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction
Methods are provided for detecting and quantitating molecules using fluidics. In preferred embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
C40B 30/00 - Procédés de criblage des bibliothèques
C40B 40/06 - Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
98.
INTEGRATED ANALYSIS DEVICES AND RELATED FABRICATION METHODS AND ANALYSIS TECHNIQUES
Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
B81B 1/00 - Dispositifs sans éléments mobiles ou flexibles, p. ex. dispositifs capillaires microscopiques
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p. ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
G01N 1/00 - ÉchantillonnagePréparation des éprouvettes pour la recherche
Methods of labeling DNA molecules comprising the use of one or more dCas proteins and one or more labeled guide RNAs (gRNAs) are described herein. In some embodiments, the labeled DNA molecules are elongated in fluidic nanochannels, where they can be analyzed.
