The present invention relates to the use of a DNA glycosylase enzyme for removing or blocking uracil containing polynucleotides, and methods incorporating the use of such enzymes to remove or block uracil containing poly nucleotides from polynucleotide containing compositions, particularly in methods of template-free DNA synthesis The present invention further relates to methods of tethering polynucleotides to a surface or molecule using a DNA glycosylase enzyme, the resulting products therefrom, and to modified DNA glycosylase enzymes.
The invention relates to a method of purifying a solution comprising newly synthetized polynucleotides, particularly in the field of polynucleotide synthesis such as enzymatic polynucleotide synthesis. The invention also relates to a device configured to carry out the purification method.
Methods, devices, and compositions are provided for inkjet-assisted synthesis and recovery of a plurality of polynucleotides at reaction sites on a substrate comprising cleavable initiator nucleic acids, using template-free polymerases. Compositions include printable stabile formulations of cleavage reagents for inkjet delivery including, but not limited to, endonucleases, pH buffer and magnesium.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
C09D 11/04 - Encres d’imprimerie à base de protéines
C09D 11/38 - Encres pour l'impression à jet d'encre caractérisées par des additifs non macromoléculaires autres que les solvants, les pigments ou les colorants
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
4.
FILTERING DEVICE AND METHOD FOR POLYNUCLEOTIDE ENZYMATIC SYNTHESIS
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12M 1/40 - Appareillage spécialement destiné à l'utilisation d'enzymes libres, immobilisées ou liées à un support, p. ex. appareils contenant un lit fluidisé d'enzymes immobilisées
The present invention relates to a method of synthesizing a polynucleotide, wherein the method comprises the steps of (a) performing a cycle comprising the steps of (i) contacting a template- independent polymerase with an initiator nucleic acid and a 3'-O-protected nucleoside triphosphate, so that the initiator nucleic acid is elongated by the template-independent polymerase activity by incorporation of the 3' -O-protected nucleoside triphosphate, to form a 3'-O-protected elongated initiator nucleic acid, (ii) deprotecting the 3'-O-protected elongated initiator nucleic acid to form an elongated nucleic acid having a free 3'- hydroxyl, (b) repeating cycles of step (a) to further elongate the initiator nucleic acid, until the polynucleotide is synthesized, wherein the template-independent polymerase is anchored to a solid support during cycles of step (a). The invention further relates to polymerases, kits, solid supports, devices for use in the invention and related uses.
The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.
The present invention is directed to methods, compositions and kits for template-free enzymatic synthesis of polynucleotides having sequences capable of forming G-quadruplex (G4) structures. In accordance with the invention elongation reactions affected by G4 formation are carried out in the presence of polyC oligonucleotides, such as polyC initiators, that inhibit or prevent formation of either intra-strand or inter-strand G4 structures.
Centre National De La Recherche Scientifique (France)
Inventeur(s)
Champion, Elise
Soskine, Mikhael
Ybert, Thomas
Delarue, Marc
Abrégé
The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.
The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.
An apparatus (500) for enzymatic synthesis of a plurality of polynucleotides, the apparatus (500) comprising: a first of a plurality of well plates (502, 902, 804, 904, 806, 906) having a first number of reaction wells: a second of the plurality of well plates (502, 902, 804, 904, 806, 906) having the first number of wells arranged to correspond to the reaction wells of the first well plate; and a vacuum transfer station (1000) configured with a plate lifting platform (1006) that is moved vertically by a horizontally driven wedge-shaped component (1004), the plate lifting platform (1006) being configured to move vertically to raise and lower the second well plate, wherein when the first well plate is moved to and placed on the vacuum transfer station (1000) above the second well plate, the wedge-shaped component (1004) raises the plate lifting platform (1006) to position the second well plate to a predetermined distance (D1, D2, D3, D4) from the first well plate.
The present invention relates to novel polypeptides having increased endonuclease V activity and uses thereof, in particular for cleaving labelled DNA.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12P 21/00 - Préparation de peptides ou de protéines
12.
STABILIZED VARIANTS OF HIGHLY ACTIVE ENDONUCLEASE V FOR CLEAVAGE OF LABELLED DNA
The present invention relates to novel polypeptides having increased endonuclease V activity and uses thereof, in particular for cleaving labelled DNA.
This disclosure relates to an enzymatic method of synthesizing a polynucleotide, comprising a deprotecting step which uses as a deprotecting agent a specific phosphonate compound. It also pertains to a method for deprotecting 3′-O-amino elongated fragments of a polynucleotide in an enzymatic method of synthesizing a polynucleotide, comprising contacting the elongated fragments with this phosphonate compound. This disclosure further relates to a kit for synthesizing a polynucleotide comprising one or more vials of synthesis reagents, at least one of which contains an effective amount of a phosphonate compound and to a specific method for preparing said phosphonate compound.
This invention relates to a 3'-O-blocked nucleotide having an aminooxymethyl blocking group. It further relates to an enzymatic method of synthesizing a polynucleotide, using this 3'-O-blocked nucleotide. This invention also pertains to a method for preparing this compound and to nucleotide and nucleoside intermediates obtained during its synthesis. This invention further relates to a kit for synthesizing a polynucleotide comprising one or more vials of synthesis reagents, at least one of which contains an effective amount of this 3'-O- blocked nucleotide or a precursor thereof.
C07H 19/073 - Radicaux pyrimidine avec un désoxy-2 ribosyle comme radical saccharide
C07H 19/173 - Radicaux purine avec un désoxy-2 ribosyle comme radical saccharide
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
15.
NOVEL TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TDT) VARIANTS AND USES THEREOF
The invention relates to novel DNA polymerases of the polX family, and in particular Terminal deoxynucleotidyl Transferase (TdT) variants comprising specific mutations or substitutions, and to their uses.
The invention states to a novel DNA polymerase of the polX family, in particular a Terminal deoxynucleotidyl Transferase (TdT) variants comprising specific mutations or substitutions and their uses.
The present application relates to methods and kits for generating a plurality of polynucleotides from a single stranded nucleic acid which comprises the plurality of polynucleotides and at least one cleavable linker comprising two different special nucleotides and using two different endonucleases, the first cleaving 5' of the first special nucleotide and the second cleaving 3' of the second special nucleotide and thereby releasing releasing scarless polynucleotides deprived of the cleavable linker.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
The present invention relates to methods for synthesizing polynucleotides, particularly on a reusable solid surface. The present invention also relates to a kit for use with said methods, and to methods for storing information.
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides using a step of addition of salt compounds during the polynucleotide's elongation.
This disclosure relates to an enzymatic method of synthesizing a polynucleotide, comprising a deprotecting step which uses as a deprotecting agent a specific phosphonate compound. It also pertains to a method for deprotecting 3′-O-amino elongated fragments of a polynucleotide in an enzymatic method of synthesizing a polynucleotide, comprising contacting the elongated fragments with this phosphonate compound. This disclosure further relates to a kit for synthesizing a polynucleotide comprising one or more vials of synthesis reagents, at least one of which contains an effective amount of a phosphonate compound and to a specific method for preparing said phosphonate compound.
This invention relates to an enzymatic method of synthesizing a polynucleotide, comprising a deprotecting step which uses as a deprotecting agent a specific phosphonate compound. It also pertains to a method for deprotecting 3'-O-amino elongated fragments of a polynucleotide in an enzymatic method of synthesizing a polynucleotide, comprising contacting the elongated fragments with this phosphonate compound. This invention further relates to a kit for synthesizing a polynucleotide comprising one or more vials of synthesis reagents, at least one of which contains an effective amount of a phosphonate compound and to a specific method for preparing said phosphonate compound.
The present invention relates to a method for generating a double-stranded nucleic acid having a predetermined sequence and a method for producing a double-stranded polynucleotide of interest. The invention further relates to a kit for the implementation of said methods.
The present invention relates to an electrochemical device (1) for enzymatic synthesis of polynucleotides, comprising at least one circulation duct (4) configured to receive an electrolytic solution, the circulation duct (4) being delimited by a first wall (6) and a second wall (7) opposite each other, the first wall (6) forming a support plate (8) of at least one reaction site configured for the enzymatic synthesis and able to carry a polynucleotide initiator fragment, the circulation duct (4) being equipped with a set of electrodes (2), the set of electrodes comprising at least one electrode of an array of electrodes (10) formed on the support plate (8) and operatively associated with the reaction sites, the set of electrodes (2) also comprising at least one additional electrode (16) arranged on the second wall (7) opposite the support plate (8).
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions
25.
ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES USING 3'-O-AMINO-2'-DEOXYRIBONUCLEOSIDE TRIPHOSPHATE MONOMERS
The invention is directed to improvements to methods of enzymatic synthesis of polynucleotides employing 3′-O-amino-nucleoside triphosphate monomers in which aldehyde scavenging agents which are O-substituted hydroxy lamines or polyhydroxylamines reduce or prevent spurious capping of growing polynucleotide chains. thereby increasing yields of full length product.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
27.
Variants of terminal deoxynucleotidyl transferase and uses thereof
Centre National de la Recherche Scientifque (France)
Inventeur(s)
Champion, Elise
Soskine, Mikhael
Ybert, Thomas
Delarue, Marc
Abrégé
The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.
Methods are provided for template-free enzymatic synthesis of polynucleotides on a solid support using double cleavage to produce scarless polynucleotides. In particular, methods are disclosed that use an initiator that comprises a cleavable group, which can be cleaved by a small molecule chemical agent, adjusting pH, heat, or photocleaved by illumination with light to release polynucleotides from a solid support after enzymatic synthesis is completed, and an enzymatic cleavage site, which can be cleaved by a cleavage enzyme to remove polynucleotide scars.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
C40B 50/14 - Synthèse en phase solide, c.-à-d. dans laquelle au moins un bloc servant à créer la bibliothèque est lié à un support solide au cours de la création de la bibliothèqueProcédés particuliers de clivage à partir du support solide
Methods, devices, and compositions are provided for inkjet-assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions include printable stabile formulations of synthesis reagents for inkjet delivery including, but not limited to, template-free polymerases, divalent cations, and nucleotides.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
B41M 1/00 - Encrage et impression avec une forme d'impression
C09D 11/38 - Encres pour l'impression à jet d'encre caractérisées par des additifs non macromoléculaires autres que les solvants, les pigments ou les colorants
C09D 11/54 - Encres à base de deux liquides, l’un des liquides étant l’encre, l’autre liquide étant une solution de réaction, un fixateur ou une solution de traitement pour l’encre
B41M 5/00 - Procédés de reproduction ou méthodes de reproduction ou de marquageMatériaux en feuilles utilisés à cet effet
30.
METHODS FOR OBTAINING CORRECTLY ASSEMBLED NUCLEIC ACIDS
Methods, systems, and devices are provided for identification and selective amplification of a correctly assembled nucleic acid comprising a sequence of interest from a mixture of assembled nucleic acids. In some aspects, the inventions use split-pool enzymatic DNA synthesis to add a bead-specific index sequence to bead-bound clonally amplified assembled nucleic acids. High-throughput sequencing is used to identify beads comprising correctly assembled, sequence-perfect nucleic acids. A correctly assembled nucleic acid may be selectively amplified using a primer comprising a sequence that is complementary to the bead-specific index sequence that is associated with the correctly assembled nucleic acid.
The present invention relates to a printing device (1) configured for the enzymatic synthesis of biomolecules, comprising a printing head (2), at least one ink reservoir (7) containing synthesis reagents and a substrate plate (16) configured to receive ink drops (6), the printing device (1) being equipped with heat treatment means (26) configured to generate a positive temperature differential between the print head (2) and/ or the ink reservoir (7) on the one hand and the substrate plate (16) on the other.
The invention is directed to formulations of terminal deoxynucleotidyl transferases (TdTs) inks employing variable viscosity modifiers and their use in inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
B41M 5/00 - Procédés de reproduction ou méthodes de reproduction ou de marquageMatériaux en feuilles utilisés à cet effet
C09D 11/38 - Encres pour l'impression à jet d'encre caractérisées par des additifs non macromoléculaires autres que les solvants, les pigments ou les colorants
B41M 1/00 - Encrage et impression avec une forme d'impression
C09D 11/328 - Encres pour l'impression à jet d'encre caractérisées par des agents colorants caractérisées par des colorants
The present invention relates to poly(A) polymerase variants having several amino acid modifications which confer increased stability, increased activity and increased purity of the polynucleotides obtained therefrom during template-free polynucleotide synthesis. The present invention further relates to use of the variant enzymes for synthesising polynucleotides, methods of synthesising polynucleotides comprising the variant enzymes, and kits comprising the variant enzyme.
The present invention relates to nucleic acid constructs capable of generating an RNA product, methods and kits for generating RNA products using said nucleic acid constructs.
The present invention relates to the use of a DNA glycosylase enzyme for removing or blocking uracil containing polynucleotides, and methods incorporating the use of such enzymes to remove or block uracil containing polynucleotides from polynucleotide containing compositions, particularly in methods of template-free DNA synthesis The present invention further relates to methods of tethering polynucleotides to a surface or molecule using a DNA glycosylase enzyme, the resulting products therefrom, and to modified DNA glycosylase enzymes.
Centre National De La Recherche Scientifique (France)
Inventeur(s)
Ybert, Thomas
Delarue, Marc
Abrégé
The invention relates to variants of a DNA polymerase of the polX family capable of synthesizing a nucleic acid molecule without a template strand, or of a functional fragment of such a polymerase, comprising at least one mutation of a residue in at least one specific position, and to uses of said variants, in particular for the synthesis of nucleic acid molecules comprising 3′-OH modified nucleotides.
Title: Method for synthesizing polynucleotides using a move-stop printer The present invention relates to a method for synthesizing polynucleotides using a move-stop printer ejecting ink droplets, comprising: a set-up step (30) during which sets of instructions are implemented, each set of instructions controlling the functioning of the move-stop printer for a row of reaction sites, each instruction of the sets of instructions controlling the opening of one nozzle; a positioning step (36); a computing step (38); a firing step (40) during which the nozzles eject droplets on at least one reaction site, according to the sets of instructions; a synthesis step (8); a moving step (47); some steps (38, 40, 8, 47) being repeated until every row of reaction sites has been placed under the nozzles of the move-stop printer.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
B41J 3/407 - Machines à écrire ou mécanismes d'impression ou de marquage sélectif caractérisés par le but dans lequel ils sont construits pour le marquage sur des matériaux particuliers
C12M 1/40 - Appareillage spécialement destiné à l'utilisation d'enzymes libres, immobilisées ou liées à un support, p. ex. appareils contenant un lit fluidisé d'enzymes immobilisées
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G16Z 99/00 - Matière non prévue dans les autres groupes principaux de la présente sous-classe
38.
Stabilized N-Terminally Truncated Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof
The present invention is directed to terminal deoxynucleotidyl transferase (TdT) variants from a variety of species which have been engineered for increase compactness and enhanced efficiency in incorporating reversibly blocked nucleoside triphosphates into a polynucleotide, especially polynucleotides attached porous solid supports. The invention includes use of such TdTs for synthesizing polynucleotides of any predetermined sequence.
The present invention relates to a printing device (1) comprising a printing head (2), a supporting platform (18) receiving a substrate plate (16), and a cover (34) for covering the printing head (2), the supporting platform (18) comprising a receiving area (32) for receiving the cover (34) and a receiving area (20) for receiving the substrate plate (16), the supporting platform (18) being movable between a first position with the receiving area (20) facing the printing head (2) and a second position with the receiving area (32) facing the printing head (2), the printing device (1) comprising reversible securing means (38) for reversibly securing the cover (34) to the printing head (2), which are activated according to the position of the supporting platform (18).
C12M 1/40 - Appareillage spécialement destiné à l'utilisation d'enzymes libres, immobilisées ou liées à un support, p. ex. appareils contenant un lit fluidisé d'enzymes immobilisées
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
The present invention relates to an enzymatic synthesis apparatus configured for the synthesis of biomolecules, comprising a support platform (18) having a receiving area configured to receive a substrate plate (16) on which ink drops containing synthesis reagents are disposed, the enzymatic synthesis apparatus comprising a cover (22) for covering the substrate plate (16), the cover (22) being equipped with reversible hooking means (40) for reversibly hooking to a frame (42) of a covering station of the enzymatic synthesis apparatus.
C12M 1/40 - Appareillage spécialement destiné à l'utilisation d'enzymes libres, immobilisées ou liées à un support, p. ex. appareils contenant un lit fluidisé d'enzymes immobilisées
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/30 - Inoculateur ou échantillonneur incorporé au récipient l'échantillonneur étant un tampon
The present invention relates to a supporting platform (18) configured to receive a substrate plate (16) on which ink drops containing biomolecules are disposed, said platform comprising a main wall (28) on which receiving means (38, 40) are formed in order to receive the substrate plate (16), a recess (32) being provided in a space in the support platform (18), extending from the main wall (28), and the receiving means (38, 40) being arranged around the recess (32) such that the substrate plate (16) is received so as to cover the recess (32).
C12M 1/40 - Appareillage spécialement destiné à l'utilisation d'enzymes libres, immobilisées ou liées à un support, p. ex. appareils contenant un lit fluidisé d'enzymes immobilisées
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
The invention relates to a method of purifying a solution comprising newly synthetized polynucleotides, particularly in the field of polynucleotide synthesis such as enzymatic polynucleotide synthesis. The invention also relates to a device configured to carry out the purification method.
The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.
A modular accessory rack (1), comprising a bottom housing (2) having at least two vertically elongated chambers (6), each extending from a bottom surface (21) of the bottom housing (2) to a top edge (22) delimiting a top opening, and at least two sub-racks (4) each removably inserted into the same or different vertically elongated chambers (6) of the bottom housing (2) via the corresponding top opening, wherein each sub-rack (4) has one or more vertically elongated receptacles for holding accessories, and wherein the bottom housing (2) has a front surface bordering the at least two vertically elongated chambers (6), the front surface having at least a U-shaped cutout (27) formed from the top edge (22) delimiting one of the vertically elongated chamber (6).
An apparatus (500) for enzymatic synthesis of a plurality of polynucleotides, the apparatus (500) comprising: a first of a plurality of well plates (502, 902, 804, 904, 806, 906) having a first number of reaction wells; a second of the plurality of well plates (502, 902, 804, 904, 806, 906) having the first number of wells arranged to correspond to the reaction wells of the first well plate; and a vacuum transfer station (1000) configured with a plate lifting platform (1006) that is moved vertically by a horizontally driven wedge-shaped component (1004), the plate lifting platform (1006) being configured to move vertically to raise and lower the second well plate, wherein when the first well plate is moved to and placed on the vacuum transfer station (1000) above the second well plate, the wedge-shaped component (1004) raises the plate lifting platform (1006) to position the second well plate to a predetermined distance (D1, D2, D3, D4) from the first well plate.
An apparatus (500) for enzymatic synthesis of a plurality of polynucleotides, the apparatus (500) comprising a chiller (11) with a condensation generating device (8) and a condensation trap (1) comprising a frame with a front surface (2) and an opposing back surface (4) connected by first and second lateral sides (6), wherein the frame houses a U-shaped inner structure that is formed by two opposing inner side surfaces (12) connected by an inner bottom surface (14), wherein the U-shaped inner structure forms a space traversing the frame between the front surface (2) and the back surface (4), the space configured to accommodate a protuberance of the condensation generating device (8), wherein the inner bottom surface (14) comprises a trough (10) configured to collect condensate from the condensation generating device (8), the trough (10) comprising a sloped surface (16) angled downward from a top edge (18) nearest the back surface (4) to a bottom edge (20) nearest the front surface (2), the bottom edge (20) connecting to a first edge (22) of a condensate channel (23), wherein the condensation trap (1) also comprises a plate (24) forming part of the front surface (2) of the frame and forming a vertical wall of the trough along a second edge of the condensate channel (23) across from the first edge (22), wherein the condensate channel (23) slopes downwards, terminating at an aperture (26), wherein the aperture (26) is in fluid communication with a tubing connector (28).
Alignment post and secure mechanism for enzymatic polynucleotide synthesis An apparatus (500) for enzymatic synthesis of a plurality of polynucleotides, the apparatus (500) comprising a reagent cabinet (516) configured to receive and 5 hold a reagent rack (540, 560) including a plurality of reagent containers, the reagent cabinet (516) including a cabinet door (514), at least one of the plurality of reagent containers containing an elongation buffer having an elongation enzyme, the reagent rack (540, 560) including a pair of positioning tabs (554, 556) that protrude from an insertion end of the reagent rack (540, 560), such that 10 in a case that the reagent rack (540, 560) is improperly inserted into the reagent cabinet (516), the positioning tabs (554, 556) prevent the cabinet door (514) from properly closing.
The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.
The present invention is directed to the use of terminal deoxynucleotidyltransferase (TdT) variants lacking dismutation acivity for template-free enzymatic synthesis of polynucleotides of any predetermined sequence. Such TdT variants permit higher yields of correct sequence polynucleotides.
The present invention relates to variant of Family A polymerases able to synthesize a nucleic acid fragment without template and to incorporate a reversible modified terminator nucleotide during the nucleic acid fragment synthesis. The present invention further relates to uses thereof for enzymatic synthesis of nucleic acid molecules.
The invention relates to variants of a DNA polymerase of the polX family capable of synthesizing a nucleic acid molecule without a template strand, or of a functional fragment of such a polymerase, comprising at least one mutation of a residue in at least one specific position, and to uses of said variants, in particular for the synthesis of nucleic acid molecules comprising 3′-OH modified nucleotides.
The present invention is directed to terminal deoxynucleotidyl transferase (TdT) variants from a variety of species which display enhanced efficiency in incorporating reversibly blocked nucleoside triphosphates into a polynucleotide, and to the use of such TdTs in synthesizing polynucleotides of any predetermined sequence.
The invention states to a novel DNA polymerase of the polX family, in particular a Terminal deoxynucleotidyl Transferase (TdT) variants comprising specific mutations or substitutions and their uses.
The invention states to a novel DNA polymerase of the polX family, in particular a Terminal deoxynucleotidyl Transferase (TdT) variants comprising specific mutations or substitutions and their uses.
The invention states to a novel DNA polymerase of the polX family, in particular a Terminal deoxynucleotidyl Transferase (TdT) variants comprising specific mutations or substitutions and their uses.
The invention is directed to methods and apparatus for parallel enzymatic synthesis of polynucleotides in an array of reaction chambers using a template-free polymerase that has sequence-dependent coupling efficiencies. Whenever sequences causing low efficiency coupling occur at a 3′ end of a growing chain of a polynucleotide being synthesized, one or more additional coupling cycles without de-protection steps are inserted into synthesis plans to provide additional time for completing the coupling reaction at that position of the polynucleotide.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes and kits comprising enzymes,
monomers, solid media, synthesis plates, all for use in
scientific research, namely, for use in synthesizing
polynucleotides, namely, for amplifying, assembling and
modifying genetic fragments, genotyping, gene modification,
and analyzing biological systems, samples and cells, in
clinical, medical and biological facilities and laboratories
using or storing nucleic acid molecules; stem cells other
than for medical or veterinary use; chemical products for
analyses in laboratories other than for medical or
veterinary use; protein [raw material]. Chemical reagents and enzymes for medical use, namely, for
use in synthesizing polynucleotides, namely, for amplifying,
assembling, modifying genetic fragments, genotyping, gene
modification and analysis of biological systems, samples and
cells, in clinical, medical and biological facilities and
laboratories using or storing nucleic acid molecules; stem
cells for medical use; stem cells for veterinary use. Laboratory apparatus and instruments and apparatus and
instruments for scientific use for polynucleotide synthesis,
namely DNA and RNA, as well as parts and accessories of such
apparatus and instruments, namely, bottles, small bottles,
cartridges, vials, reaction plates, synthesis supports,
reaction tubes, as well as computer hardware and
downloadable computer software relating thereto for use in
operating and interfacing with apparatus and instruments for
synthesizing polynucleotides; laboratory apparatus and
instruments and apparatus and instruments for scientific use
using or storing nucleic acid molecules, particularly for
amplification, assembly or modification of genetic
fragments, genotyping, gene modification and analysis of
biological cells, samples or systems; DNA chips; apparatus
for scientific use for preservation, storage or processing
of DNA chips containing information; computer hardware and
computer software for use in apparatus and instruments for
synthesizing polynucleotides; computer hardware and computer
software for polynucleotide synthesis, namely, DNA and RNA;
computer hardware and computer software for use in
amplifying, assembling or modifying genetic fragments,
genotyping, gene modification and analysis of biological
cells, samples or systems; computer hardware and computer
software for use in preserving, storing or processing DNA
chips containing information; biochips; DNA chips. DNA and RNA testing apparatus for medical use; testing
apparatus for medical use; diagnostic apparatus for medical
use; analysis devices for identification of bacteria for
medical use. Administration of loyalty and reward programs; business
management and business advisory services; marketing
consultancy services; market study; promotion of the sale of
third-party goods and services by means of coupons,
discounts, advertisements, rewards and incentives generated
by the use of electronic links to websites for merchants and
retailers and promotional contests; promotion of third-party
goods and services through the distribution of printed
matter and advertisements; advertising; commercial
administration; clerical services (office functions);
assistance services for the management of industrial and
commercial companies; commercial business appraisal;
advisory services for business management; market study;
commercial statistical information; drawing up of statements
of accounts; accounting; business research; public relations
services; publication of advertising texts; publication of
publicity leaflets; computerized file management services;
processing of information and checking of information; data
retrieval services; organization of exhibitions for
commercial or advertising purposes; provision of information
concerning the purchase of goods and services on line via
the Internet and other computer networks; profiling
information directory of customer data; advisory and
information services in the aforesaid fields; administrative
services for medical referrals; commercial administration of
the licensing of third-party goods and services;
administration of consumer loyalty programs; providing user
appraisals for commercial or advertising purposes;
compilation of information indexes for commercial or
advertising purposes; compilation of information into
computer databases; providing user reviews for commercial or
advertising purposes; providing business information;
providing information with respect to business and
commercial contacts; providing commercial information and
advice to consumers regarding choice of goods and services. Technical evaluations concerning design (engineers'
services); scientific research; technical research;
biological research; chemical research; medical research;
chemical analysis; chemistry services; scientific laboratory
services; designing and testing synthetic DNA for scientific
research purposes; polynucleotide synthesis services,
namely, DNA and RNA for scientific research purposes;
preserving, storing or processing DNA chips containing
information; design of computers for third parties; computer
development; software design; software development; research
and development of new products for third parties;
conducting of technical project studies; software
development (design); software installation; software
maintenance; updating of software; software rental;
programming for computers; computer system analysis;
computer system design; consultant services with respect to
computer hardware design and development; digitization of
documents; Software as a Service (SaaS); cloud computing;
advice regarding information technology; server hosting;
graphic arts design services; styling (industrial design);
authentication of works of art; energy auditing; electronic
data storage; storing nucleic acid molecules, particularly
for amplifying, assembling or modifying genetic fragments,
genotyping, gene modification and analysis of biological
cells, samples or systems. Medical services; veterinary services; skin care services
(beauty and sanitary care); medical assistance; plastic
surgery; hospital services; medical analysis services
provided by medical laboratories for diagnostic and
therapeutic purposes; advice with respect to health;
consultation with respect to pharmacy.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes and kits comprising enzymes,
monomers, solid media, synthesis plates, all for use in
scientific research, namely, for use in synthesizing
polynucleotides, namely, for amplifying, assembling and
modifying genetic fragments, genotyping, gene modification,
and analyzing biological systems, samples and cells, in
clinical, medical and biological facilities and laboratories
using or storing nucleic acid molecules; stem cells other
than for medical or veterinary use; chemical products for
analyses in laboratories other than for medical or
veterinary use; protein [raw material]. Chemical reagents and enzymes for medical use, namely, for
use in synthesizing polynucleotides, namely, for amplifying,
assembling, modifying genetic fragments, genotyping, gene
modification, and analysis of biological systems, samples
and cells, in clinical, medical and biological facilities
and laboratories using or storing nucleic acid molecules;
stem cells for medical use; stem cells for veterinary use. Laboratory apparatus and instruments and apparatus and
instruments for scientific use for polynucleotide synthesis,
namely DNA and RNA, as well as parts and accessories for
such apparatus and instruments, namely, bottles, flasks,
cartridges, vials, reaction plates, synthesis supports,
reaction tubes, as well as computer hardware and
downloadable computer software relating thereto for use in
operating and interfacing with apparatus and instruments for
synthesizing polynucleotides; laboratory apparatus and
instruments and apparatus and instruments for scientific use
using or storing nucleic acid molecules, particularly for
amplification, assembly or modification of genetic
fragments, genotyping, gene modification and analysis of
biological cells, samples or systems; DNA chips; apparatus
for scientific use for preservation, storage or processing
of DNA chips containing information; computer hardware and
computer software for use in apparatus and instruments for
synthesizing polynucleotides; computer hardware and computer
software for synthesis of polynucleotides, namely DNA and
RNA; computer hardware and computer software for use in
amplifying, assembling or modifying genetic fragments,
genotyping, gene modification and analysis of biological
cells, samples or systems; computer hardware and computer
software for use in preserving, storing or processing DNA
chips containing information; biochips; DNA chips. DNA and RNA testing apparatus for medical use; testing
apparatus for medical use; diagnostic apparatus for medical
use; analysis devices for identification of bacteria for
medical use. Technical evaluations concerning design (engineers'
services); scientific research; technical research;
biological research; chemical research; medical research;
chemical analysis; chemistry services; scientific laboratory
services; synthetic DNA design and testing services for
scientific research purposes; polynucleotide synthesis
services, namely, DNA and RNA for scientific research
purposes; storing nucleic acid molecules, particularly for
amplifying, assembling or modifying genetic fragments,
genotyping, gene modification and analysis of biological
cells, samples or systems; preserving, storing or processing
DNA chips containing information; design of computers for
third parties; computer development; software design;
software development; research and development of new
products for third parties; conducting of technical project
studies; software development (design); software
installation; software maintenance; updating of software;
software rental; programming for computers; computer system
analysis; computer system design; consultant services with
respect to computer hardware design and development;
digitization of documents; Software as a Service (SaaS);
cloud computing; advice regarding information technology;
server hosting; graphic arts design services; styling
(industrial design); authentication of works of art; energy
auditing; electronic data storage. Medical services; veterinary services; skin care services
(beauty and sanitary care); medical assistance; plastic
surgery; hospital services; medical analysis services
provided by medical laboratories for diagnostic and
therapeutic purposes; advice with respect to health;
consultation with respect to pharmacy.
61.
Chimeric Terminal Deoxynucleotidyl Transferases For Template-Free Enzymatic Synthesis Of Polynucleotides
The invention is directed to compositions and methods for enzymatic template-free synthesis of polynucleotides using terminal deoxynucleotidyl transferases that are chimeras derived from variants from different species.
The present invention relates to a method for generating a double-stranded nucleic acid having a predetermined sequence and a method for producing a double-stranded polynucleotide of interest. The invention further relates to a kit for the implementation of said methods.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes and kits comprising enzymes,
monomers, solid media, synthesis plates, all for use in
scientific research, namely, for use in synthesizing
polynucleotides, namely, for amplifying, assembling and
modifying genetic fragments, genotyping, gene modification,
and analyzing biological systems, samples and cells, in
clinical, medical and biological facilities and laboratories
using or storing nucleic acid molecules; stem cells other
than for medical or veterinary use; chemical products for
analyses in laboratories other than for medical or
veterinary use; protein [raw material]; all the aforesaid
goods other than those directly for use in the
cardiovascular field. Chemical reagents and enzymes for medical use, namely, for
use in synthesizing polynucleotides, namely, for amplifying,
assembling, modifying genetic fragments, genotyping, gene
modification, and analysis of biological systems, samples
and cells, in clinical, medical and biological facilities
and laboratories using or storing nucleic acid molecules;
stem cells for medical use; stem cells for veterinary use;
all the aforesaid goods other than those directly for use in
the cardiovascular field. Laboratory apparatus and instruments and apparatus and
instruments for scientific use for polynucleotide synthesis,
namely DNA and RNA, as well as parts and accessories for
such apparatus and instruments, namely, bottles, flasks,
cartridges, vials, reaction plates, synthesis supports,
reaction tubes, as well as computer hardware and
downloadable computer software relating thereto for use in
operating and interfacing with apparatus and instruments for
synthesizing polynucleotides; laboratory apparatus and
instruments and apparatus and instruments for scientific use
using or storing nucleic acid molecules, particularly for
amplification, assembly or modification of genetic
fragments, genotyping, gene modification and analysis of
biological cells, samples or systems; DNA chips; apparatus
for scientific use for preservation, storage or processing
of DNA chips containing information; computer hardware and
computer software for use in apparatus and instruments for
synthesizing polynucleotides; computer hardware and computer
software for polynucleotide synthesis, namely, DNA and RNA;
computer hardware and computer software for use in
amplifying, assembling or modifying genetic fragments,
genotyping, gene modification and analysis of biological
cells, samples or systems; computer hardware and computer
software for use in preserving, storing or processing DNA
chips containing information; biochips; DNA chips; all the
aforesaid goods other than those directly for use in the
cardiovascular field. DNA and RNA testing apparatus for medical use; testing
apparatus for medical use; diagnostic apparatus for medical
use; analysis devices for identification of bacteria for
medical use; all the aforesaid goods other than those
directly for use in the cardiovascular field. Technical evaluations concerning design (engineers'
services); scientific research; technical research;
biological research; chemical research; medical research;
chemical analysis; chemistry services; scientific laboratory
services; designing and testing synthetic DNA for scientific
research purposes; polynucleotide synthesis services,
namely, DNA and RNA for scientific research purposes;
computer services for storing nucleic acid molecules,
particularly for amplification, assembly or modification of
genetic fragments, genotyping, gene modification and
analysis of cells, samples or biological systems on DNA
chips containing information; biological research laboratory
services for storage of nucleic acid molecules, particularly
for amplification, assembly or modification of genetic
fragments, genotyping, gene modification and analysis of
cells, samples or biological systems on DNA chips containing
information; chemical laboratory services for storage of
nucleic acid molecules, particularly for amplification,
assembly or modification of genetic fragments, genotyping,
gene modification and analysis of cells, samples or
biological systems on DNA chips containing information;
scientific services for the storage of nucleic acid
molecules, particularly for the amplification, assembly or
modification of genetic fragments, genotyping, gene
modification and analysis of cells, samples or biological
systems on DNA chips containing information; computer
services for the preservation, storage or processing of data
on DNA chips containing information; biological research
laboratory services for preservation, storage or processing
of data on DNA chips containing information; chemical
laboratory services for preservation, storage or processing
of data on DNA chips containing information; scientific
services for the preservation, storage or processing of data
on DNA chips containing information; ; design of computers
for third parties; computer development; software design;
software development; research and development of new
products for third parties; conducting of technical project
studies; software development (design); software
installation; software maintenance; updating of software;
software rental; programming for computers; computer system
analysis; computer system design; consultant services with
respect to computer hardware design and development;
digitization of documents; Software as a Service (SaaS);
cloud computing; advice regarding information technology;
server hosting; graphic arts design services; styling
(industrial design); authentication of works of art; energy
auditing; electronic data storage; all the aforesaid
services other than those directly for use in the
cardiovascular field. Medical services; veterinary services; skin care services
(beauty and sanitary care); medical assistance; plastic
surgery; hospital services; medical analysis services
provided by medical laboratories for diagnostic and
therapeutic purposes; advice with respect to health;
consultation with respect to pharmacy; all the aforesaid
services other than those directly for use in the
cardiovascular field.
64.
High Efficiency Template-Free Enzymatic Synthesis of Polynucleotides
The invention is directed to compositions and methods for enzymatic template-free synthesis of polynucleotides wherein different terminal deoxynucleotidyl transferase (TdT) variants are used to incorporate different 3′-O-reversibly blocked deoxynucleoside triphosphates (dNTPs) into a growing chain. In part, the invention is a recognition and appreciation that different TdT variants can be engineered to preferentially incorporate specific dNTPs with higher efficiency than a single “general purpose” TdT variant used to incorporate all four 3-O-reversibly blocked dNTPs.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes and kits comprising enzymes,
monomers, solid media, synthesis plates, all for use in
scientific research, namely, for use in synthesizing
polynucleotides, namely, for amplifying, assembling and
modifying genetic fragments, genotyping, gene modification,
and analyzing biological systems, samples and cells, in
clinical, medical and biological facilities and laboratories
using or storing nucleic acid molecules; stem cells other
than for medical or veterinary use; chemical products for
analyses in laboratories other than for medical or
veterinary use; protein [raw material]. Chemical reagents and enzymes for medical use, namely, for
use in synthesizing polynucleotides, namely, for amplifying,
assembling, modifying genetic fragments, genotyping, gene
modification and analysis of biological systems, samples and
cells, in clinical, medical and biological facilities and
laboratories using or storing nucleic acid molecules; stem
cells for medical use; stem cells for veterinary use. Laboratory apparatus and instruments and apparatus and
instruments for scientific use for polynucleotide synthesis,
namely DNA and RNA, as well as parts and accessories of such
apparatus and instruments, namely, bottles, small bottles,
cartridges, vials, reaction plates, synthesis supports,
reaction tubes, as well as computer hardware and
downloadable computer software relating thereto for use in
operating and interfacing with apparatus and instruments for
synthesizing polynucleotides; laboratory apparatus and
instruments and apparatus and instruments for scientific use
using or storing nucleic acid molecules, particularly for
amplification, assembly or modification of genetic
fragments, genotyping, gene modification and analysis of
biological cells, samples or systems; DNA chips; apparatus
for scientific use for preservation, storage or processing
of DNA chips containing information; computer hardware and
computer software for use in apparatus and instruments for
synthesizing polynucleotides; computer hardware and computer
software for polynucleotide synthesis, namely, DNA and RNA;
computer hardware and computer software for use in
amplifying, assembling or modifying genetic fragments,
genotyping, gene modification and analysis of biological
cells, samples or systems; computer hardware and computer
software for use in preserving, storing or processing DNA
chips containing information; biochips; DNA chips. DNA and RNA testing apparatus for medical use; testing
apparatus for medical use; diagnostic apparatus for medical
use; analysis devices for identification of bacteria for
medical use. Administration of loyalty and reward programs; business
management and business advisory services; marketing
consultancy services; market study; promotion of the sale of
third-party goods and services by means of coupons,
discounts, advertisements, rewards and incentives generated
by the use of electronic links to websites for merchants and
retailers and promotional contests; promotion of third-party
goods and services through the distribution of printed
matter and advertisements; advertising; commercial
administration; clerical services (office functions);
assistance services for the management of industrial and
commercial companies; commercial business appraisal;
advisory services for business management; market study;
commercial statistical information; drawing up of statements
of accounts; accounting; business research; public relations
services; publication of advertising texts; publication of
publicity leaflets; computerized file management services;
processing of information and checking of information; data
retrieval services; organization of exhibitions for
commercial or advertising purposes; provision of information
concerning the purchase of goods and services on line via
the Internet and other computer networks; profiling
information directory of customer data; advisory and
information services in the aforesaid fields; administrative
services for medical referrals; commercial administration of
the licensing of third-party goods and services;
administration of consumer loyalty programs; providing user
appraisals for commercial or advertising purposes;
compilation of information indexes for commercial or
advertising purposes; compilation of information into
computer databases; providing user reviews for commercial or
advertising purposes; providing business information;
providing information with respect to business and
commercial contacts; providing commercial information and
advice to consumers regarding choice of goods and services. Technical evaluations concerning design (engineers'
services); scientific research; technical research projects
and studies; biological research; chemical research; medical
research; chemical analysis; chemistry services; scientific
laboratory services; designing and testing synthetic DNA for
scientific research purposes; polynucleotide synthesis
services, namely, DNA and RNA for scientific research
purposes; storing nucleic acid molecules, particularly for
amplifying, assembling or modifying genetic fragments,
genotyping, gene modification and analysis of biological
cells, samples or systems; preserving, storing or processing
DNA chips containing information; design of computers for
third parties; computer development; software design;
software development; research and development of new
products for others; conducting of technical project
studies; software development (design); software
installation; software maintenance; updating of software;
software rental; programming for computers; computer system
analysis; computer system design; consultant services with
respect to computer hardware design and development;
digitization of documents; Software as a Service (SaaS);
cloud computing; advice regarding information technology;
server hosting; graphic arts design services; styling
(industrial design); authentication of works of art; energy
auditing; electronic data storage. Medical services; veterinary services; skin care services
(beauty and sanitary care); medical assistance; plastic
surgery; hospital services; medical analysis services
provided by medical laboratories for diagnostic and
therapeutic purposes; advice with respect to health;
consultation with respect to pharmacy.
66.
Increasing Long-Sequence Yields In Template-Free Enzymatic Synthesis of Polynucleotides
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides using chain elongation conditions that suppress the formation of DNA secondary structures including, but not limited to, intra-strand and between-strand duplexes, G-quadruplexes, and the like. In some embodiments, such chain elongation conditions include using 3′-O-blocked dNTP monomers that base protection groups or base analogs that suppress the formation of hydrogen bonding in the polynucleotide being synthesized.
The present invention is directed to methods and compositions for template-free enzymatic synthesis of a polyribonucleotide of a predetermined sequence from 3′-O-reversibly blocked nucleoside triphosphates using poly(A) and poly(U) polymerases.
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides employing hybridization stringency and/or nuclease digestion for removing failure sequences.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
Centre National de la Recherche Scientifique (France)
Inventeur(s)
Champion, Elise
Soskine, Mikhael
Ybert, Thomas
Delarue, Marc
Abrégé
The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2. 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.
The invention is directed to improvements to methods of enzymatic synthesis of polynucleotides employing 3'-O-amino-nucleoside triphosphate monomers in which aldehyde scavenging agents which are O-substituted hydroxylamines or polyhydroxylamines reduce or prevent spurious capping of growing polynucleotide chains, thereby increasing yields of full length product.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Produits chimiques, réactifs, enzymes et kits comprenant des enzymes, monomères, supports solides, plaques de synthèse, tous à utiliser dans la recherche scientifique, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de et stockant des molécules d'acides nucléiques; cellules souches pour la recherche; cellules souches à usage scientifique; réactifs chimiques pour la recherche génétique; réactif de culture cellulaire pour l'utilisation laboratoire; protéines sous forme de matières premières pour la recherche scientifique.
(2) Réactifs chimiques et enzymes à usage médical, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de et stockant des molécules d'acides nucléiques; cellules souches à usage médical; cellules souches à usage vétérinaire.
(3) Appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; accessoires pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir bouteilles, flacons, cartouches, fioles, plaques de réaction, supports de synthèse, tubes de réaction; logiciels informatiques téléchargeables pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; appareils et instruments de laboratoire et appareils et instruments à usage scientifique utilisant et stockant des molécules d'acide nucléique, nommément pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; puces à ADN; appareils à usage scientifique pour la conservation, le stockage et le traitement de puces à ADN contenant des informations; matériel informatique et logiciels informatiques destinés à des appareils et instruments pour la synthétisation de polynucléotides; matériel informatique et logiciels informatiques pour la synthèse de polynucléotides, à savoir l'ADN et l'ARN; matériel informatique et logiciels informatiques destinés à l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; matériel informatique et logiciels informatiques destinés à la conservation, le stockage et le traitement de puces à ADN contenant des informations; biopuces.
(4) Appareils médicaux pour l'amplification de molécules d'acide nucléique ADN et ARN; appareil médical d'essais génétiques pour l'identification de séquences d'ADN et d'ARN spécifiques de genres et d'espèces; dispositifs de détection de biomolécules de diagnostic et de séquences d'ADN à des fins médicales cliniques; dispositifs d'analyse pour l'identification de bactéries à usage médical. (1) Évaluation de la conception du produit; recherches scientifique dans le domaine de la génétique et du génie génétique; recherches technologiques dans le domaine de la génétique et du génie génétique; recherches biologiques; recherches en chimie; recherches médicales; analyse chimique; services de chimiste; services de laboratoires scientifiques, nommément, laboratoires chimiques, de diagnostics médicaux et de recherche scientifique dans le domaine de la génétique et du génie génétique; services de conception et de test d'ADN synthétique à des fins de recherche scientifique; services de synthèse de polynucléotides, à savoir l'ADN et l'ARN à des fins de recherche scientifique; services de stockage des molécules d'acide nucléique, nommément pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; services de conservation, de stockage et de traitement de puces à ADN contenant des informations; conception d'ordinateurs pour des tiers; développement d'ordinateurs; conception de logiciels; développement de logiciels; recherche et développement de nouveaux produits pour des tiers; conduite d'études de projets techniques dans le domaine de la génétique et du génie génétique; élaboration (conception) de logiciels; installation de logiciels; maintenance de logiciels; mise à jour de logiciels; location de logiciels informatiques; programmation pour ordinateurs; analyse de systèmes informatiques; conception de systèmes informatiques; services de conseillers en matière de conception et de développement de matériel informatique; numérisation de documents; logiciel-service (SaaS) pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; fournisseur d'informatique en nuage pour le stockage de données; services de conseil en technologie informatique; services d'hébergement de serveurs; services de conception d'art graphique; services de conception industrielle; authentification d'oeuvres d'art; audits en matière d'énergie; stockage électronique de données pour l'archivage de dossiers personnels, de dossiers médicaux et des résultats de recherches scientifiques dans le domaine de la génétique et du génie génétique.
(2) Services médicaux, nommément services d'analyses d'ADN à des fins médicales, d'administration de tests médicaux, d'examens médicaux à des fins de diagnostic ou de traitement, d'examens médicaux en matière de diagnostic et de traitement des maladies, services médicaux dans les domaine de l'oncologie et d'administration de tests génétiques à des fins médicales; services vétérinaires; assistance médicale, nommément fourniture d'informations médicales dans le domaine de la génétique et du génie génétique; chirurgie esthétique; services hospitaliers; services d'analyses médicales fournis par des laboratoires médicaux à des fins diagnostiques et thérapeutiques; consultation génétique; consultation en matière de pharmacie.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes, and kits comprising enzymes, nucleotide monomers, solid phase synthesis media, and synthesis plates for laboratory use, all for use in scientific research, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling and modifying genetic fragments, genotyping, gene modification, and analyzing biological systems, samples and cells, in the course of scientific research conducted in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells other than for medical or veterinary use; chemical preparations for analyses in laboratories other than for medical or veterinary use; protein in raw material form for scientific researcb Chemical reagents and enzymes for medical use, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling, modifying genetic fragments, genotyping, gene modification, and analysis of biological systems, samples and cells, in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells for medical use; stem cells for veterinary use Apparatus and instruments for laboratory and scientific use for polynucleotide synthesis in the field of DNA and RNA, namely, pipetors, liquid handlers, laboratory columns for chemical synthesis, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory research instruments for DNA analysis, as well as parts and accessories for such apparatus and instruments, namely, laboratory glassware in the nature of bottles, flasks, cartridges, vials, reaction plates, synthesis supports, and reaction tubes, as well as computer hardware and downloadable computer software relating thereto for use in operating and interfacing with apparatus and instruments for synthesizing polynucleotides; apparatus and instruments for laboratory and scientific use in using or storing nucleic acid molecules, particularly for amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems, namely, laboratory devices for detecting genetic sequences, scientific apparatus and instruments for measuring relative DNA, RNA and protein, electrical equipment for the genetic modification of cells for research purposes, laboratory apparatus and instruments for extracting nucleic acid; DNA chips; apparatus for scientific use for preservation, storage or processing of DNA chips containing information, namely, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory apparatus and instruments for extracting nucleic acid; computer hardware and recorded and downloadable computer software for use in apparatus and instruments for synthesizing polynucleotides; computer hardware and recorded and downloadable computer software for synthesis of polynucleotides, namely, DNA and RNA; computer hardware and recorded and downloadable computer software for use in amplifying, assembling or modifying genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems; computer hardware and recorded and downloadable computer software for use in preserving, storing or processing DNA chips containing information; biochips for scientific research purposes and data storage purposes DNA and RNA testing apparatus for medical use; DNA sample testing apparatus for medical use; diagnostic apparatus for medical use for the detection of genetic particularities, mutations and diseases; analysis devices for identification of bacteria for medical use Technical evaluations concerning design being engineers' services; scientific research; technical research in the field of polynucleotide synthesis; biological research; chemical research; medical research; chemical analysis; chemistry services, namely, research and laboratory analysis in the field of chemistry; scientific laboratory services; designing and testing synthetic DNA for scientific research purposes; polynucleotide synthesis services, namely, DNA and RNA synthesis for scientific research purposes; scientific and medical laboratory services, namely, encoding or processing DNA chips containing information and data; design of computers for third parties; computer hardware development; software design; software development; research and development of new products for third parties; technical research in the field of DNA and RNA, namely, conducting of technical project studies; software development and design; software installation; software maintenance; updating of software; software rental, namely, rental of computer software for operating apparatus and instruments for DNA and RNA synthesis; computer programming for computers; computer system analysis; computer system design; consultant services with respect to computer hardware design and development; digitization of documents; Software as a Service (SaaS) featuring software for operating apparatus and instruments for DNA and RNA synthesis; cloud computing featuring software for operating apparatus and instruments for DNA and RNA synthesis; advice regarding information technology, namely, installation, maintenance and troubleshooting of computer software; hosting of servers; graphic arts design services; styling being industrial design services; authentication of works of art; energy auditing; electronic data storage Medical services; veterinary services; cosmetic and medical skin care services for beauty and sanitary care purposes; medical assistance; plastic surgery; hospital services; medical analysis services provided by medical laboratories for diagnostic and treatment purposes; advice with respect to health in the field of DNA and its preservation; consultation with respect to pharmaceuticals
The invention is directed to methods for synthesizing a plurality of oligonucleotides in the same reaction vessel, and in some embodiments, using the synthesized oligonucleotides in an oligonucleotide-based assay in such reaction vessel. In some embodiments, methods of the invention are implemented by steps of (a) providing a plurality of different initiators attached to one or more supports, each different initiator having a terminal nucleotide with a different 3-O-blocking group; (b) for each different initiator, synthesizing a polynucleotide by repeated cycles of template-free enzymatic additions of 3′-O-blocked nucleoside triphosphates, wherein the blocking group of the 3-O-blocked nucleoside triphosphate is removable under deblocking conditions orthogonal to the deblocking conditions for removing blocking groups of the other initiators; and (c) releasing the oligonucleotides from the polynucleotides and the one or more solid supports.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
74.
Template-Free Enzymatic Polynucleotide Synthesis Using Photocleavable Linkages
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides employing photocleavable linkages. In some embodiments, such methods include using 3′-O—NH2-dNTP monomers which may react with photocleavage products having free ketone to allow synthesis and purification on the same or an added support.
The present invention is directed to methods, compositions and kits for template-free enzymatic synthesis of polynucleotides having sequences capable of forming G-quadruplex (G4) structures. In accordance with the invention elongation reactions affected by G4 formation are carried out in the presence of polyC oligonucleotides, such as polyC initiators, that inhibit or prevent formation of either intra-strand or inter-strand G4 structures.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes and kits comprising enzymes,
monomers, solid media, synthesis plates, all for use in
scientific research, namely, for use in synthesizing
polynucleotides, namely, for amplifying, assembling and
modifying genetic fragments, genotyping, gene modification,
and analyzing biological systems, samples and cells, in
clinical, medical and biological facilities and laboratories
using or storing nucleic acid molecules; stem cells other
than for medical or veterinary use; chemical products for
analyses in laboratories other than for medical or
veterinary use; protein [raw material]. Chemical reagents and enzymes for medical use, namely, for
use in synthesizing polynucleotides, namely, for amplifying,
assembling, modifying genetic fragments, genotyping, gene
modification, and analysis of biological systems, samples
and cells, in clinical, medical and biological facilities
and laboratories using or storing nucleic acid molecules;
stem cells for medical use; stem cells for veterinary use. Laboratory apparatus and instruments and apparatus and
instruments for scientific use for polynucleotide synthesis,
namely, DNA and RNA, as well as parts and accessories of
such apparatus and instruments, namely, bottles, flasks,
cartridges, vials, reaction plates, synthesis media,
reaction tubes, as well as related computer hardware and
software for use in the use and interfacing with apparatus
and instruments for synthesizing polynucleotides; laboratory
apparatus and instruments and apparatus and instruments for
scientific use using or storing nucleic acid molecules,
particularly for amplification, assembly or modification of
genetic fragments, genotyping, gene modification and
analysis of biological cells, samples or systems; DNA chips;
apparatus for scientific use for preservation, storage or
processing of DNA chips containing information; computer
hardware and computer software for use in apparatus and
instruments for synthesizing polynucleotides; computer
hardware and computer software for polynucleotide synthesis,
namely, DNA and RNA; computer hardware and computer software
for use in amplifying, assembling or modifying genetic
fragments, genotyping, gene modification and analysis of
biological cells, samples or systems; computer hardware and
computer software for use in preserving, storing or
processing DNA chips containing information; biochips; DNA
chips. DNA and RNA testing apparatus for medical use; testing
apparatus for medical use; diagnostic apparatus for medical
use; analysis devices for identification of bacteria for
medical use. Technical evaluations concerning design (engineers'
services); scientific research; technical research;
biological research; chemical research; medical research;
chemical analysis; chemistry services; scientific laboratory
services; designing and testing synthetic DNA for scientific
research purposes; polynucleotide synthesis services,
namely, DNA and RNA for scientific research purposes;
storing nucleic acid molecules, particularly for amplifying,
assembling or modifying genetic fragments, genotyping, gene
modification and analysis of biological cells, samples or
systems; preserving, storing or processing DNA chips
containing information; design of computers for third
parties; computer development; software design; software
development; research and development of new products for
third parties; conducting of technical project studies;
software development (design); software installation;
software maintenance; updating of software; software rental;
programming for computers; computer system analysis;
computer system design; consultant services with respect to
computer hardware design and development; digitization of
documents; Software as a Service (SaaS); cloud computing;
advice regarding information technology; hosting of servers;
graphic arts design services; styling (industrial design);
authentication of works of art; energy auditing; electronic
data storage. Medical services; veterinary services; skin care services
(beauty and sanitary care); medical assistance; plastic
surgery; hospital services; medical analysis services
provided by medical laboratories for diagnostic and
therapeutic purposes; advice with respect to health;
consultation with respect to pharmacy.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes and kits comprising enzymes,
monomers, solid media, synthesis plates, all for use in
scientific research, namely, for use in synthesizing
polynucleotides, namely, for amplifying, assembling and
modifying genetic fragments, genotyping, gene modification,
and analyzing biological systems, samples and cells, in
clinical, medical and biological facilities and laboratories
using or storing nucleic acid molecules; stem cells other
than for medical or veterinary use; chemical products for
analyses in laboratories other than for medical or
veterinary use; protein [raw material]. Chemical reagents and enzymes for medical use, namely, for
use in synthesizing polynucleotides, namely, for amplifying,
assembling, modifying genetic fragments, genotyping, gene
modification, and analysis of biological systems, samples
and cells, in clinical, medical and biological facilities
and laboratories using or storing nucleic acid molecules;
stem cells for medical use; stem cells for veterinary use. Laboratory apparatus and instruments and apparatus and
instruments for scientific use for polynucleotide synthesis,
namely, DNA and RNA, as well as parts and accessories of
such apparatus and instruments, namely, bottles, flasks,
cartridges, vials, reaction plates, synthesis media,
reaction tubes, as well as related computer hardware and
software for use in the use and interfacing with apparatus
and instruments for synthesizing polynucleotides; laboratory
apparatus and instruments and apparatus and instruments for
scientific use using or storing nucleic acid molecules,
particularly for amplification, assembly or modification of
genetic fragments, genotyping, gene modification and
analysis of biological cells, samples or systems; DNA chips;
apparatus for scientific use for preservation, storage or
processing of DNA chips containing information; computer
hardware and computer software for use in apparatus and
instruments for synthesizing polynucleotides; computer
hardware and computer software for polynucleotide synthesis,
namely, DNA and RNA; computer hardware and computer software
for use in amplifying, assembling or modifying genetic
fragments, genotyping, gene modification and analysis of
biological cells, samples or systems; computer hardware and
computer software for use in preserving, storing or
processing DNA chips containing information; biochips; DNA
chips. DNA and RNA testing apparatus for medical use; testing
apparatus for medical use; diagnostic apparatus for medical
use; analysis devices for identification of bacteria for
medical use. Technical evaluations concerning design (engineers'
services); scientific research; technical research;
biological research; chemical research; medical research;
chemical analysis; chemistry services; scientific laboratory
services; designing and testing synthetic DNA for scientific
research purposes; polynucleotide synthesis services,
namely, DNA and RNA for scientific research purposes;
storing nucleic acid molecules, particularly for amplifying,
assembling or modifying genetic fragments, genotyping, gene
modification and analysis of biological cells, samples or
systems; preserving, storing or processing DNA chips
containing information; design of computers for third
parties; computer development; software design; software
development; research and development of new products for
third parties; conducting of technical project studies;
software development (design); software installation;
software maintenance; updating of software; software rental;
programming for computers; computer system analysis;
computer system design; consultant services with respect to
computer hardware design and development; digitization of
documents; Software as a Service (SaaS); cloud computing;
advice regarding information technology; hosting of servers;
graphic arts design services; styling (industrial design);
authentication of works of art; energy auditing; electronic
data storage. Medical services; veterinary services; skin care services
(beauty and sanitary care); medical assistance; plastic
surgery; hospital services; medical analysis services
provided by medical laboratories for diagnostic and
therapeutic purposes; advice with respect to health;
consultation with respect to pharmacy.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes and kits comprising enzymes,
monomers, solid media, synthesis plates, all for use in
scientific research, namely, for use in synthesizing
polynucleotides, namely, for amplifying, assembling and
modifying genetic fragments, genotyping, gene modification,
and analyzing biological systems, samples and cells, in
clinical, medical and biological facilities and laboratories
using or storing nucleic acid molecules; stem cells other
than for medical or veterinary use; chemical products for
analyses in laboratories other than for medical or
veterinary use; protein [raw material]. Chemical reagents and enzymes for medical use, namely, for
use in synthesizing polynucleotides, namely, for amplifying,
assembling, modifying genetic fragments, genotyping, gene
modification, and analysis of biological systems, samples
and cells, in clinical, medical and biological facilities
and laboratories using or storing nucleic acid molecules;
stem cells for medical use; stem cells for veterinary use. Laboratory apparatus and instruments and apparatus and
instruments for scientific use for polynucleotide synthesis,
namely, DNA and RNA, as well as parts and accessories of
such apparatus and instruments, namely, bottles, flasks,
cartridges, vials, reaction plates, synthesis media,
reaction tubes, as well as related computer hardware and
software for use in the use and interfacing with apparatus
and instruments for synthesizing polynucleotides; laboratory
apparatus and instruments and apparatus and instruments for
scientific use using or storing nucleic acid molecules,
particularly for amplification, assembly or modification of
genetic fragments, genotyping, gene modification and
analysis of biological cells, samples or systems; DNA chips;
apparatus for scientific use for preservation, storage or
processing of DNA chips containing information; computer
hardware and computer software for use in apparatus and
instruments for synthesizing polynucleotides; computer
hardware and computer software for polynucleotide synthesis,
namely, DNA and RNA; computer hardware and computer software
for use in amplifying, assembling or modifying genetic
fragments, genotyping, gene modification and analysis of
biological cells, samples or systems; computer hardware and
computer software for use in preserving, storing or
processing DNA chips containing information; biochips; DNA
chips. DNA and RNA testing apparatus for medical use; testing
apparatus for medical use; diagnostic apparatus for medical
use; analysis devices for identification of bacteria for
medical use. Technical evaluations concerning design (engineers'
services); scientific research; technical research;
biological research; chemical research; medical research;
chemical analysis; chemistry services; scientific laboratory
services; designing and testing synthetic DNA for scientific
research purposes; polynucleotide synthesis services,
namely, DNA and RNA for scientific research purposes;
storing nucleic acid molecules, particularly for amplifying,
assembling or modifying genetic fragments, genotyping, gene
modification and analysis of biological cells, samples or
systems; preserving, storing or processing DNA chips
containing information; design of computers for third
parties; computer development; software design; software
development; research and development of new products for
third parties; conducting of technical project studies;
software development (design); software installation;
software maintenance; updating of software; software rental;
programming for computers; computer system analysis;
computer system design; consultant services with respect to
computer hardware design and development; digitization of
documents; Software as a Service (SaaS); cloud computing;
advice regarding information technology; hosting of servers;
graphic arts design services; styling (industrial design);
authentication of works of art; energy auditing; electronic
data storage. Medical services; veterinary services; skin care services
(beauty and sanitary care); medical assistance; plastic
surgery; hospital services; medical analysis services
provided by medical laboratories for diagnostic and
therapeutic purposes; advice with respect to health;
consultation with respect to pharmacy.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Produits chimiques, réactifs, enzymes et kits comprenant des enzymes, monomères, supports solides, plaques de synthèse, tous à utiliser dans la recherche scientifique, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de ou stockant des molécules d'acides nucléiques; cellules souches pour la recherche et à usage scientifique; produits chimiques pour analyses en laboratoire autres qu'à usage médical ou vétérinaire, nommément réactif chimiques pour la recherche génétique; protéine [matière première] nommément enzymes polymérases de l'ADN; tous les produits précités à l'exception de ceux directement destinés au domaine cardiovasculaire.
(2) Réactifs chimiques et enzymes à usage médical, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de molécules d'acides nucléiques; Réactifs chimiques et enzymes à usage médical, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques stockant des molécules d'acides nucléiques; cellules souches à usage médical; cellules souches à usage vétérinaire; tous les produits précités à l'exception de ceux directement destinés au domaine cardiovasculaire.
(3) Appareils et instruments de laboratoire et appareils et instruments à usage scientifique pour la synthèse de polynucléotides, à savoir synthétiseurs d'ADN et synthétiseurs d'ARN, ainsi que les parties et accessoires de ces appareils et instruments, à savoir bouteilles de laboratoire, flacons de laboratoire, cartouches pour synthétiseurs d'ADN et synthétiseurs d'ARN, fioles de laboratoire, plaques de réaction optique, supports de synthèse solides physiques et chimiques optimisés nommément colonnes chromatographiques de laboratoires, tubes de réaction, matériel informatique et logiciels informatiques téléchargeables permettant l'utilisation synthétiseurs d'ADN et synthétiseurs d'ARN, la connexion et l'intégration de synthétiseurs d'ADN et synthétiseurs d'ARN avec des appareils et instruments pour la synthétisation de polynucléotides; appareils et instruments de laboratoire et appareils et instruments à usage scientifique utilisant et stockant des molécules d'acide nucléique, pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; puces à ADN; appareils à usage scientifique pour la conservation, le stockage et le traitement de puces à ADN contenant des informations; matériel informatique et logiciels informatiques destinés à des appareils et instruments pour la synthétisation de polynucléotides; matériel informatique et logiciels informatiques pour la synthèse de polynucléotides, à savoir l'ADN et l'ARN; matériel informatique et logiciels informatiques destinés à l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; matériel informatique et logiciels informatiques destinés à la conservation, le stockage et le traitement de puces à ADN contenant des informations; biopuces; puces à ADN; tous les produits précités à l'exception de ceux directement destinés au domaine cardiovasculaire.
(4) Appareils à usage médical d'essais génétiques pour l'identification de séquences ADN et ARN; appareils pour l'analyse à usage médical, nommément lecteurs de biomolécules diagnostiques et de séquences d'ADN de résultats de tests de biomolécules et de séquences d'ADN pour une utilisation médicale clinique; appareils pour le diagnostic à usage médical, nommément dispositifs de détection de biomolécules de diagnostic et de séquences d'ADN à des fins médicales cliniques; dispositifs d'analyse pour l'identification de bactéries à usage médical; tous les produits précités à l'exception de ceux directement destinés au domaine cardiovasculaire. (1) Évaluations techniques concernant la conception, nommément réalisation d'études de faisabilité techniques, d'analyses et d'évaluations dans le domaine de la génétique, de la génomique, de la biologie, du séquençage de l'ADN et de l'ARN; recherches scientifiques dans le domaine de la génétique, de la génomique, de la biologie, du séquençage de l'ADN et de l'ARN; recherches techniques dans le domaine de la génétique, de la génomique, de la biologie, du séquençage de l'ADN et de l'ARN; recherches biologiques; recherches en chimie; recherches médicales dans le domaine de la génétique, de la génomique, de la biologie, du séquençage de l'ADN et de l'ARN; analyse chimique; services de chimie, nommément recherche et conception dans le domaine de la chimie, établissement de rapports en rapport avec la recherche en chimie; services de laboratoires de recherche scientifiques dans le domaine de la génétique, de la génomique, de la biologie, du séquençage de l'ADN et de l'ARN; services de conception et de test d'ADN synthétique à des fins de recherche scientifique; services de synthèse de polynucléotides, à savoir l'ADN et l'ARN à des fins de recherche scientifique; services informatiques pour le stockage des molécules d'acide nucléique, pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques sur des puces à ADN contenant des informations; services de laboratoire de recherches biologiques pour le stockage des molécules d'acide nucléique, pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques sur des puces à ADN contenant des informations; services de laboratoires chimiques pour le stockage des molécules d'acide nucléique, pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques sur des puces à ADN contenant des informations; services scientifiques pour le stockage des molécules d'acide nucléique, pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques sur des puces à ADN contenant des informations; services informatiques permettant l'archivage, le stockage, l'analyse et la collecte de données sur des puces à ADN contenant des séquences d'ADN; services de laboratoire de recherches biologiques dans le domaine de la conservation, du stockage et du traitement de données biologiques sur des puces à ADN contenant des informations; services de laboratoires chimiques permettant la conservation, le stockage ou le traitement de données sur des puces à ADN contenant des informations; services scientifiques permettant la conservation, le stockage ou le traitement de données sur des puces à ADN contenant des informations, nommément séquençages d'ADN à des fins de recherches médicales; recherche et développement de nouveaux produits pour des tiers; conduite d'études de projets techniques dans le domaine de la génétique, de la génomique, de la biologie, du séquençage de l'ADN et de l'ARN; logiciel-service (SaaS) à savoir fourniture d'un logiciel permettant d'accéder à des informations stockées dans des bases de données dans le domaine de la génétique, de la génomique, de la biologie, du séquençage de l'ADN et de l'ARN; informatique en nuage permettant l'archivage de fichiers contenant des données ADN; services de stylisme et esthétique industrielle, nommément services de design industriel technologique; authentification d'oeuvres d'art; audits en matière d'énergie; stockage électronique de données pour l'archivage de données biologiques sur des puces à ADN; tous les services précités à l'exception de ceux directement destinés au domaine cardiovasculaire.
(2) Services médicaux, nommément analyses d'ADN à des fins médicales; services vétérinaires; services pour le soin de la peau nommément, services de soins esthétiques pour la peau; assistance médicale nommément services de soins de relève sous forme de services d'assistance en soins infirmiers; chirurgie esthétique; services de cliniques et d'hôpitaux; conseils en matière de santé mentale, de santé au travail et de la nutrition; tous les services précités à l'exception de ceux directement destinés au domaine cardiovasculaire.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes, and kits comprising enzymes, nucleotide monomers, solid phase synthesis media, and synthesis plates for laboratory use, all for use in scientific research, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling and modifying genetic fragments, genotyping, gene modification, and analyzing biological systems, samples and cells, in the course of scientific research conducted in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells other than for medical or veterinary use; chemical preparations for analyses in laboratories other than for medical or veterinary use; protein in raw material form for scientific research; all the aforesaid goods other than those directly for use in the cardiovascular field Chemical reagents and enzymes for medical use, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling, modifying genetic fragments, genotyping, gene modification, and analysis of biological systems, samples and cells, in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells for medical use; stem cells for veterinary use; all the aforesaid goods other than those directly for use in the cardiovascular field Apparatus and instruments for laboratory and scientific use for polynucleotide synthesis in the field of DNA and RNA, namely, pipetors, liquid handlers, laboratory columns for chemical synthesis, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory research instruments for DNA analysis, as well as parts and accessories for such apparatus and instruments, namely, laboratory glassware in the nature of bottles, flasks, cartridges, vials, reaction plates, synthesis supports, and reaction tubes, as well as computer hardware and downloadable computer software relating thereto for use in operating and interfacing with apparatus and instruments for synthesizing polynucleotides; apparatus and instruments for laboratory and scientific use in using or storing nucleic acid molecules, particularly for amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems, namely, laboratory devices for detecting genetic sequences, scientific apparatus and instruments for measuring relative DNA, RNA and protein, electrical equipment for the genetic modification of cells for research purposes, laboratory apparatus and instruments for extracting nucleic acid; DNA chips; Apparatus for scientific use for preservation, storage or processing of DNA chips containing information, namely, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory apparatus and instruments for extracting nucleic acid; computer hardware and recorded and downloadable computer software for use in apparatus and instruments for synthesizing polynucleotides; computer hardware and recorded and downloadable computer software for polynucleotide synthesis, namely, DNA and RNA; computer hardware and recorded and downloadable computer software for use in amplifying, assembling or modifying genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems; computer hardware and recorded and downloadable computer software for use in preserving, storing or processing DNA chips containing information; biochips for scientific research purposes and data storage purposes; all the aforesaid goods other than those directly for use in the cardiovascular field DNA and RNA testing apparatus for medical use; DNA sample testing apparatus for medical use; diagnostic apparatus for medical use for the detection of genetic particularities, mutations and diseases; analysis devices for identification of bacteria for medical use; all the aforesaid goods other than those directly for use in the cardiovascular field Technical research in the field of polynucleotide synthesis; biological research; chemical research; medical research; chemical analysis; chemistry services, namely, research and laboratory analysis in the field of chemistry; scientific laboratory services; designing and testing synthetic DNA for scientific research purposes; polynucleotide synthesis services, namely, DNA and RNA synthesis for scientific research purposes; computer services for storing nucleic acid molecules, particularly, cloud storage services for electronic data comprising visual representations of nucleic acid molecules for use in amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of cells, samples or biological systems on DNA chips containing information; biological research laboratory services in the field of nucleic acid molecules, particularly for amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of cells, samples or biological systems on DNA chips containing information; chemical laboratory services in the field of nucleic acid molecules, particularly for amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of cells, samples or biological systems on DNA chips containing information; scientific research and laboratory services in the field of nucleic acid molecules, particularly for the amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of cells, samples or biological systems on DNA chips containing information; computer services for the preservation, storage or processing of data on DNA chips containing information, namely, providing temporary use of online non-downloadable computer software for preserving, storing and processing data in the field of DNA; biological research laboratory services for preservation or processing of data on DNA chips containing information; chemical laboratory services for preservation or processing of data on DNA chips containing information; scientific research and laboratory services for the preservation or processing of data on DNA chips containing information; technical research in the field of DNA and RNA, namely, conducting of technical project studies; software development and design; software rental, namely, rental of computer software for operating apparatus and instruments for DNA and RNA synthesis; digitization of documents; Software as a Service (SaaS) featuring software for operating apparatus and instruments for DNA and RNA synthesis; cloud computing featuring software for operating apparatus and instruments for DNA and RNA synthesis; graphic arts design services; styling being industrial design services; authentication of works of art; energy auditing; all the aforesaid services other than those directly for use in the cardiovascular field Medical services; veterinary services; cosmetic and medical skin care services for beauty and sanitary care purposes; medical assistance; plastic surgery; hospital services; medical analysis services provided by medical laboratories for diagnostic and treatment purposes; advice with respect to health in the field of DNA and its preservation; consultation with respect to pharmaceuticals; all the aforesaid services other than those directly for use in the cardiovascular field
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Produits chimiques, réactifs, enzymes et kits comprenant des enzymes, monomères, supports solides, plaques de synthèse, tous à utiliser dans la recherche scientifique, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de et stockant des molécules d'acides nucléiques; cellules souches pour la recherche; cellules souches à usage scientifique; réactifs chimiques pour la recherche génétique; réactif de culture cellulaire pour l'utilisation laboratoire; protéines sous forme de matières premières pour la recherche scientifique.
(2) Réactifs chimiques et enzymes à usage médical, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de et stockant des molécules d'acides nucléiques; cellules souches à usage médical; cellules souches à usage vétérinaire.
(3) Appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; accessoires pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir bouteilles, flacons, cartouches, fioles, plaques de réaction, supports de synthèse, tubes de réaction; logiciels informatiques pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; appareils et instruments de laboratoire et appareils et instruments à usage scientifique utilisant et stockant des molécules d'acide nucléique, nommément pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; puces à ADN; appareils à usage scientifique pour la conservation, le stockage et le traitement de puces à ADN contenant des informations; matériel informatique et logiciels informatiques destinés à des appareils et instruments pour la synthétisation de polynucléotides; matériel informatique et logiciels informatiques pour la synthèse de polynucléotides, à savoir l'ADN et l'ARN; matériel informatique et logiciels informatiques destinés à l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; matériel informatique et logiciels informatiques destinés à la conservation, le stockage et le traitement de puces à ADN contenant des informations; biopuces.
(4) Appareils médicaux pour l'amplification de molécules d'acide nucléique ADN et ARN; appareil médical d'essais génétiques pour l'identification de séquences d'ADN et d'ARN spécifiques de genres et d'espèces; dispositifs de détection de biomolécules de diagnostic et de séquences d'ADN à des fins médicales cliniques; dispositifs d'analyse pour l'identification de bactéries à usage médical. (1) Évaluation de la conception du produit; recherches scientifique dans le domaine de la génétique et du génie génétique; recherches technologiques dans le domaine de la génétique et du génie génétique; recherches biologiques; recherches en chimie; recherches médicales; analyse chimique; services de chimiste; services de laboratoires scientifiques, nommément, laboratoires chimiques, de diagnostics médicaux et de recherche scientifique dans le domaine de la génétique et du génie génétique; services de conception et de test d'ADN synthétique à des fins de recherche scientifique; services de synthèse de polynucléotides, à savoir l'ADN et l'ARN à des fins de recherche scientifique; services de stockage des molécules d'acide nucléique, nommément pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; services de conservation, de stockage et de traitement de puces à ADN contenant des informations; conception d'ordinateurs pour des tiers; développement d'ordinateurs; conception de logiciels; développement de logiciels; recherche et développement de nouveaux produits pour des tiers; conduite d'études de projets techniques dans le domaine de la génétique et du génie génétique; élaboration (conception) de logiciels; installation de logiciels; maintenance de logiciels; mise à jour de logiciels; location de logiciels informatiques; programmation pour ordinateurs; analyse de systèmes informatiques; conception de systèmes informatiques; services de conseillers en matière de conception et de développement de matériel informatique; numérisation de documents; logiciel-service (SaaS) pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; fournisseur d'informatique en nuage pour le stockage de données; services de conseil en technologie informatique; services d'hébergement de serveurs; services de conception d'art graphique; services de conception industrielle; authentification d'oeuvres d'art; audits en matière d'énergie; stockage électronique de données pour l'archivage de dossiers personnels, de dossiers médicaux et des résultats de recherches scientifiques dans le domaine de la génétique et du génie génétique.
(2) Services médicaux, nommément services d'analyses d'ADN à des fins médicales, d'administration de tests médicaux, d'examens médicaux à des fins de diagnostic ou de traitement, d'examens médicaux en matière de diagnostic et de traitement des maladies, services médicaux dans les domaine de l'oncologie et d'administration de tests génétiques à des fins médicales; services vétérinaires; assistance médicale, nommément fourniture d'informations médicales dans le domaine de la génétique et du génie génétique; chirurgie esthétique; services hospitaliers; services d'analyses médicales fournis par des laboratoires médicaux à des fins diagnostiques et thérapeutiques; consultation génétique; consultation en matière de pharmacie.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Produits chimiques, réactifs, enzymes et kits comprenant des enzymes, monomères, supports solides, plaques de synthèse, tous à utiliser dans la recherche scientifique, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de et stockant des molécules d'acides nucléiques; cellules souches pour la recherche; cellules souches à usage scientifique; réactifs chimiques pour la recherche génétique; réactif de culture cellulaire pour l'utilisation laboratoire; protéines sous forme de matières premières pour la recherche scientifique.
(2) Réactifs chimiques et enzymes à usage médical, à savoir à utiliser dans la synthétisation de polynucléotides, à savoir pour l'amplification, l'assemblage, la modification de fragments génétiques, le génotypage, la modification de gènes, et l'analyse de systèmes, échantillons et cellules biologiques, dans des installations et laboratoires cliniques, médicaux et biologiques à l'aide de et stockant des molécules d'acides nucléiques; cellules souches à usage médical; cellules souches à usage vétérinaire.
(3) Appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; accessoires pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir bouteilles, flacons, cartouches, fioles, plaques de réaction, supports de synthèse, tubes de réaction; logiciels informatiques pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; appareils et instruments de laboratoire et appareils et instruments à usage scientifique utilisant et stockant des molécules d'acide nucléique, nommément pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; puces à ADN; appareils à usage scientifique pour la conservation, le stockage et le traitement de puces à ADN contenant des informations; matériel informatique et logiciels informatiques destinés à des appareils et instruments pour la synthétisation de polynucléotides; matériel informatique et logiciels informatiques pour la synthèse de polynucléotides, à savoir l'ADN et l'ARN; matériel informatique et logiciels informatiques destinés à l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; matériel informatique et logiciels informatiques destinés à la conservation, le stockage et le traitement de puces à ADN contenant des informations; biopuces.
(4) Appareils médicaux pour l'amplification de molécules d'acide nucléique ADN et ARN; appareil médical d'essais génétiques pour l'identification de séquences d'ADN et d'ARN spécifiques de genres et d'espèces; dispositifs de détection de biomolécules de diagnostic et de séquences d'ADN à des fins médicales cliniques; dispositifs d'analyse pour l'identification de bactéries à usage médical. (1) Évaluation de la conception du produit; recherches scientifique dans le domaine de la génétique et du génie génétique; recherches technologiques dans le domaine de la génétique et du génie génétique; recherches biologiques; recherches en chimie; recherches médicales; analyse chimique; services de chimiste; services de laboratoires scientifiques, nommément, laboratoires chimiques, de diagnostics médicaux et de recherche scientifique dans le domaine de la génétique et du génie génétique; services de conception et de test d'ADN synthétique à des fins de recherche scientifique; services de synthèse de polynucléotides, à savoir l'ADN et l'ARN à des fins de recherche scientifique; services de stockage des molécules d'acide nucléique, nommément pour l'amplification, l'assemblage et la modification de fragments génétiques, le génotypage, la modification de gènes et l'analyse de cellules, échantillons et systèmes biologiques; services de conservation, de stockage et de traitement de puces à ADN contenant des informations; conception d'ordinateurs pour des tiers; développement d'ordinateurs; conception de logiciels; développement de logiciels; recherche et développement de nouveaux produits pour des tiers; conduite d'études de projets techniques dans le domaine de la génétique et du génie génétique; élaboration (conception) de logiciels; installation de logiciels; maintenance de logiciels; mise à jour de logiciels; location de logiciels informatiques; programmation pour ordinateurs; analyse de systèmes informatiques; conception de systèmes informatiques; services de conseillers en matière de conception et de développement de matériel informatique; numérisation de documents; logiciel-service (SaaS) pour appareils et instruments de laboratoire à usage scientifique pour effectuer la synthèse de polynucléotides, à savoir l'ADN et l'ARN; fournisseur d'informatique en nuage pour le stockage de données; services de conseil en technologie informatique; services d'hébergement de serveurs; services de conception d'art graphique; services de conception industrielle; authentification d'oeuvres d'art; audits en matière d'énergie; stockage électronique de données pour l'archivage de dossiers personnels, de dossiers médicaux et des résultats de recherches scientifiques dans le domaine de la génétique et du génie génétique.
(2) Services médicaux, nommément services d'analyses d'ADN à des fins médicales, d'administration de tests médicaux, d'examens médicaux à des fins de diagnostic ou de traitement, d'examens médicaux en matière de diagnostic et de traitement des maladies, services médicaux dans les domaine de l'oncologie et d'administration de tests génétiques à des fins médicales; services vétérinaires; assistance médicale, nommément fourniture d'informations médicales dans le domaine de la génétique et du génie génétique; chirurgie esthétique; services hospitaliers; services d'analyses médicales fournis par des laboratoires médicaux à des fins diagnostiques et thérapeutiques; consultation génétique; consultation en matière de pharmacie.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes, and kits comprising enzymes, nucleotide monomers, solid phase synthesis media, and synthesis plates for laboratory use, all for use in scientific research, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling and modifying genetic fragments, genotyping, gene modification, and analyzing biological systems, samples and cells, in the course of scientific research conducted in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells other than for medical or veterinary use; chemical preparations for analyses in laboratories other than for medical or veterinary use; protein in raw material form for scientific research Chemical reagents and enzymes for medical use, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling, modifying genetic fragments, genotyping, gene modification, and analysis of biological systems, samples and cells, in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells for medical use; stem cells for veterinary use Apparatus and instruments for laboratory and scientific use for polynucleotide synthesis in the field of DNA and RNA, namely, pipetors, liquid handlers, laboratory columns for chemical synthesis, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory research instruments for DNA analysis, as well as parts and accessories for such apparatus and instruments, namely, laboratory glassware in the nature of bottles, flasks, cartridges, vials, reaction plates, synthesis supports, and reaction tubes, as well as computer hardware and downloadable computer software relating thereto for use in operating and interfacing with apparatus and instruments for synthesizing polynucleotides; Apparatus and instruments for laboratory and scientific use in using or storing nucleic acid molecules, particularly for amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems, namely, laboratory devices for detecting genetic sequences, scientific apparatus and instruments for measuring relative DNA, RNA and protein, electrical equipment for the genetic modification of cells for research purposes, laboratory apparatus and instruments for extracting nucleic acid; DNA chips; apparatus for scientific use for preservation, storage or processing of DNA chips containing information, namely, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory apparatus and instruments for extracting nucleic acid; computer hardware and recorded and downloadable computer software for use in apparatus and instruments for synthesizing polynucleotides; computer hardware and recorded and downloadable computer software for polynucleotide synthesis, namely, DNA and RNA; computer hardware and recorded and downloadable computer software for use in amplifying, assembling or modifying genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems; computer hardware and recorded and downloadable computer software for use in preserving, storing or processing DNA chips containing information; biochips for scientific research purposes and data storage purposes DNA and RNA testing apparatus for medical use; DNA sample testing apparatus for medical use ; diagnostic apparatus for medical use for the detection of genetic particularities, mutations and diseases; analysis devices for identification of bacteria for medical use Technical evaluations concerning design being engineers' services; scientific research; technical research in the field of polynucleotide synthesis; biological research; chemical research; medical research; chemical analysis; chemistry services, namely, research and laboratory analysis in the field of chemistry; scientific laboratory services; designing and testing synthetic DNA for scientific research purposes; polynucleotide synthesis services, namely, DNA and RNA synthesis for scientific research purposes; scientific and medical laboratory services, namely, encoding or processing DNA chips containing information and data; design of computers for third parties; computer hardware development; software design; software development; research and development of new products for third parties; technical research in the field of DNA and RNA, namely, conducting of technical project studies; software development and design; software installation; software maintenance; updating of software; software rental, namely, rental of computer software for operating apparatus and instruments for DNA and RNA synthesis; computer programming for computers; computer system analysis; computer system design; consultant services with respect to computer hardware design and development; digitization of documents; Software as a Service (SaaS) featuring software for operating apparatus and instruments for DNA and RNA synthesis; cloud computing featuring software for operating apparatus and instruments for DNA and RNA synthesis; advice regarding information technology, namely, installation, maintenance and troubleshooting of computer software; hosting of servers; graphic arts design services; styling being industrial design services; authentication of works of art; energy auditing; electronic data storage Medical services; veterinary services; cosmetic and medical skin care services for beauty and sanitary care purposes; medical assistance; plastic surgery; hospital services; medical analysis services provided by medical laboratories for diagnostic and treatment purposes; advice with respect to health in the field of DNA and its preservation; consultation with respect to pharmaceuticals
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Chemicals, reagents, enzymes, and kits comprising enzymes, nucleotide monomers, solid phase synthesis media, and synthesis plates for laboratory use, all for use in scientific research, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling and modifying genetic fragments, genotyping, gene modification, and analyzing biological systems, samples and cells, in the course of scientific research conducted in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells other than for medical or veterinary use; chemical preparations for analyses in laboratories other than for medical or veterinary use; protein in raw material form for scientific research Chemical reagents and enzymes for medical use, namely, for use in synthesizing polynucleotides, namely, for amplifying, assembling, modifying genetic fragments, genotyping, gene modification, and analysis of biological systems, samples and cells, in clinical, medical and biological facilities and laboratories using or storing nucleic acid molecules; stem cells for medical use; stem cells for veterinary use Apparatus and instruments for laboratory and scientific use for polynucleotide synthesis in the field of DNA and RNA, namely, pipetors, liquid handlers, laboratory columns for chemical synthesis, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory research instruments for DNA analysis, as well as parts and accessories for such apparatus and instruments, namely, laboratory glassware in the nature of bottles, flasks, cartridges, vials, reaction plates, synthesis supports, and reaction tubes, as well as computer hardware and downloadable computer software relating thereto for use in operating and interfacing with apparatus and instruments for synthesizing polynucleotides; Apparatus and instruments for laboratory and scientific use in using or storing nucleic acid molecules, particularly for amplification, assembly or modification of genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems, namely, laboratory devices for detecting genetic sequences, scientific apparatus and instruments for measuring relative DNA, RNA and protein, electrical equipment for the genetic modification of cells for research purposes, laboratory apparatus and instruments for extracting nucleic acid; DNA chips; apparatus for scientific use for preservation, storage or processing of DNA chips containing information, namely, scientific apparatus and instruments for measuring relative DNA, RNA and protein, laboratory apparatus and instruments for extracting nucleic acid; computer hardware and recorded and downloadable computer software for use in apparatus and instruments for synthesizing polynucleotides; computer hardware and recorded and downloadable computer software for polynucleotide synthesis, namely, DNA and RNA; computer hardware and recorded and downloadable computer software for use in amplifying, assembling or modifying genetic fragments, genotyping, gene modification and analysis of biological cells, samples or systems; computer hardware and recorded and downloadable computer software for use in preserving, storing or processing DNA chips containing information; biochips for scientific research purposes and data storage purposes DNA and RNA testing apparatus for medical use; DNA sample testing apparatus for medical use ; diagnostic apparatus for medical use for the detection of genetic particularities, mutations and diseases; analysis devices for identification of bacteria for medical use Technical evaluations concerning design being engineers' services; scientific research; technical research in the field of polynucleotide synthesis; biological research; chemical research; medical research; chemical analysis; chemistry services, namely, research and laboratory analysis in the field of chemistry; scientific laboratory services; designing and testing synthetic DNA for scientific research purposes; polynucleotide synthesis services, namely, DNA and RNA synthesis for scientific research purposes; scientific and medical laboratory services, namely, encoding or processing DNA chips containing information and data; design of computers for third parties; computer hardware development; software design; software development; research and development of new products for third parties; technical research in the field of DNA and RNA, namely, conducting of technical project studies; software development and design; software installation; software maintenance; updating of software; software rental, namely, rental of computer software for operating apparatus and instruments for DNA and RNA synthesis; computer programming for computers; computer system analysis; computer system design; consultant services with respect to computer hardware design and development; digitization of documents; Software as a Service (SaaS) featuring software for operating apparatus and instruments for DNA and RNA synthesis; cloud computing featuring software for operating apparatus and instruments for DNA and RNA synthesis; advice regarding information technology, namely, installation, maintenance and troubleshooting of computer software; hosting of servers; graphic arts design services; styling being industrial design services; authentication of works of art; energy auditing; electronic data storage Medical services; veterinary services; cosmetic and medical skin care services for beauty and sanitary care purposes; medical assistance; plastic surgery; hospital services; medical analysis services provided by medical laboratories for diagnostic and treatment purposes; advice with respect to health in the field of DNA and its preservation; consultation with respect to pharmaceuticals
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
87.
STABILIZED N-TERMINALLY TRUNCATED TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE VARIANTS AND USES THEREOF
The present invention is directed to terminal deoxynucleotidyl transferase (TdT) variants from a variety of species which have been engineered for increase compactness and enhanced efficiency in incorporating reversibly blocked nucleoside triphosphates into a polynucleotide, especially polynucleotides attached porous solid supports. The invention includes use of such TdTs for synthesizing polynucleotides of any predetermined sequence.
The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3'-O-protected dNTP monomers.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
B41M 5/00 - Procédés de reproduction ou méthodes de reproduction ou de marquageMatériaux en feuilles utilisés à cet effet
C09D 11/38 - Encres pour l'impression à jet d'encre caractérisées par des additifs non macromoléculaires autres que les solvants, les pigments ou les colorants
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
89.
Terminal deoxynucleotidyl transferase variants and uses thereof
The present invention is directed to terminal deoxynucleotidyltransferase (TdT) variants that (i) comprise an amino acid sequence that is at least a specified percent identical to an indicated SEQ ID NOs and have at least one substitution at Q455 or at least Q455 plus at least one further substitution at G186, S248, T331, Q390, K394 or H466 (where positions are with respect to SEQ ID NO 1 and functionally equivalent positions in indicated SEQ ID NOs), (ii) are capable of template-free extension of a polynucleotide, and (iii) exhibit enhanced stability or enhanced efficiency in incorporating 3′-0-blocked nucleoside triphosphates into a polynucleotide. The invention is also directed to the use of these TdT variants for synthesizing polynucleotides of any predetermined sequence.
The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.
The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.
The present invention is directed to terminal deoxynucleotidyl transferase (TdT) variants from a variety of species which display enhanced efficiency in incorporating reversibly blocked nucleoside triphosphates into a polynucleotide, and to the use of such TdTs in synthesizing polynucleotides of any predetermined sequence.
The invention relates to a method for preparing 3'-O-amino-2'-deoxyribonucleoside- 5'-triphosphates with reduced 3'-hydroxy-2'-deoxyribonucleoside-5'-triphosphate contamination by converting 3'-(N-acetone-oxime)-2'-deoxynucleoside triphosphate to 3'-O- amine-2'-deoxynucleoside triphosphate by treatment with an aryl-oxyamine.
The invention is directed to methods and apparatus for parallel enzymatic synthesis of polynucleotides in an array of reaction chambers using a template-free polymerase that has sequence-dependent coupling efficiencies. Whenever sequences causing low efficiency coupling occur at a 3' end of a growing chain of a polynucleotide being synthesized, one or more additional coupling cycles without de-protection steps are inserted into synthesis plans to provide additional time for completing the coupling reaction at that position of the polynucleotide.
Centre National de la Recherche Scientifique (France)
Inventeur(s)
Sarac, Ivo
Hollenstein, Marcel
Abrégé
The invention relates to a method for preparing 3′-O-amino-2′-deoxyribonucleoside-5′-triphosphate. It also relates to a solid support functionalized with at least one N-hydroxyphthalimide moiety and uses thereof for protecting 3′-hydroxy group of a 2′-deoxyribonucleoside during synthesis of a nucleoside or a derivative thereof.
C07H 1/04 - Introduction de radicaux d'acide polyphosphorique
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
96.
TEMPLATE-FREE ENZYMATIC POLYNUCLEOTIDE SYNTHESIS USING DISMUTATIONLESS TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE VARIANTS
The present invention is directed to the use of terminal deoxynucleotidyltransferase (TdT) variants lacking dismutation acivity for template-free enzymatic synthesis of polynucleotides of any predetermined sequence. Such TdT variants permit higher yields of correct sequence polynucleotides.
The invention is directed to compositions and methods for enzymatic template-free synthesis of polynucleotides using terminal deoxynucleotidyl transferases that are chimeras derived from variants from different species.
The invention is directed to compositions and methods for enzymatic template-free synthesis of polynucleotides wherein different terminal deoxynucleotidyl transferase (TdT) variants are used to incorporate different 3'-O-reversibly blocked deoxynucleoside triphosphates (dNTPs) into a growing chain. In part, the invention is a recognition and appreciation that different TdT variants can be engineered to preferentially incorporate specific dNTPs with higher efficiency than a single "general purpose" TdT variant used to incorporate all four 3'-O-reversibly blocked dNTPs.
The invention relates to a method for synthesising long nucleic acids, including at least one cycle of elongating initial fragments of nucleic acids, including a) a phase comprising the enzymatic addition of nucleotides to said fragments, b) a phase comprising the purification of the fragments having a correct sequence, c) an optional phase of enzymatic amplification, each cycle being performed in a reaction medium which is compatible with enzymatic addition and amplification, such as an aqueous medium, the synthesis method also comprising, at the end of all the elongation cycles, a last step of final amplification. The invention also relates to the use of the method for the production of genes, or sequences of synthetic nucleic acids, DNA or RNA. The invention further relates to a kit for implementing said method.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
100.
INCREASING LONG-SEQUENCE YIELDS IN TEMPLATE-FREE ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides employing hybridization stringency and/or nuclease digestion for removing failure sequences.
