A fluid transport device, a fluid transport method, and a biochemical substance analysis apparatus. The fluid transport device comprises a chip, a fluid storage assembly and a driving assembly, wherein the driving assembly and the fluid storage assembly are both in communication with the chip; and the driving assembly uses negative-pressure driving to input a fluid in the fluid storage assembly into the chip, uses negative-pressure driving to suck out the fluid, which has been subjected to a reaction, in the chip, and uses positive-pressure driving to discharge the sucked-out fluid into the fluid storage assembly. The fluid transport device of the present application has a high degree of integration. The fluid is located in the device, and does not come into contact with the biochemical substance analysis apparatus during use, thus simplifying the structure of the apparatus, avoiding damage to the apparatus, reducing the cost, reducing the complexity of an experimental process, and increasing the experimental success rate.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
2.
SEQUENCING SYSTEMS AND METHODS UTILIZING THREE- DIMENSIONAL SUBSTRATES
A nucleic acid sequencing system may include a substrate including a three-dimensionally patterned surface. The three-dimensionally patterned surface may define nanowells each including a derivitized area for binding to nucleic acid template molecules. The nanowells may be 100 nm in diameter with 350 nm center-to-center spacing. The substrate may including reflective layers and plasmonically enhanced layers for increasing fluorescent signals during nucleic acid sequencing.
An integrated slide, a liquid changing apparatus, and a biochemical substance analysis system and analysis method. The integrated slide comprises a biochip and an ink-jet chip packaged above the biochip, the biochip and the ink-jet chip defining a biochemical reaction chamber; the ink-jet chip is communicated with the biochemical reaction chamber and is capable of adding into the biochemical reaction chamber a reagent needed by a biochemical reaction, or discharging from the biochemical reaction chamber a residual reagent which has undergone a reaction; the biological chip can implement in-situ signal detection. In the present application, the biochemical substance analysis system using the integrated slide can implement an integrated operation of sample addition, biochemical substance analysis and imaging, thereby simplifying the system structure, reducing reagent losses, helping to improve detection efficiency and reducing detection costs.
G01F 1/64 - Mesure du débit volumétrique ou du débit massique d'un fluide ou d'un matériau solide fluent, dans laquelle le fluide passe à travers un compteur par un écoulement continu en utilisant des effets électriques ou magnétiques en mesurant des courants électriques passant à travers l’écoulement de fluideMesure du débit volumétrique ou du débit massique d'un fluide ou d'un matériau solide fluent, dans laquelle le fluide passe à travers un compteur par un écoulement continu en utilisant des effets électriques ou magnétiques en mesurant le potentiel électrique produit par l’écoulement de fluide, p. ex. par effet électrochimique, effet de contact ou effet de frottement
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
A biochemical substance analysis system, a biochemical substance analysis method, a sequencing system and a sequencing method. The biochemical substance analysis system comprises a biochemical substance analysis platform and a transfer device. The biochemical substance analysis platform comprises a liquid change device, an imaging detection device and at least one biochemical reaction device. The transfer device transfers a test chip between different sites, the sites comprising a reaction site, a liquid change site and an imaging site. The test chip comprises an open-type slide capable of immobilizing a sample to be tested, and the liquid change device is used for adding a liquid to the test chip. The biochemical reaction device is used for enabling the sample in the test chip to react. The imaging detection device is used for executing imaging detection analysis on the test chip which has undergone a reaction. The biochemical substance analysis system provided by the present application can achieve integrated operation of sample adding, biochemical substance analysis and imaging, thus simplifying the system structure, reducing reagent loss, improving test efficiency and reducing test costs.
Described herein are high coverage single tube Long Fragment Read (stLFR) technology which uses performs stLFR on target DNA fragments that have already been amplified before they are co-barcoded, which provides higher amount of DNA for sequencing and increases sequencing coverage. In some embodiments, the high coverage stLFR described in this application uses two rounds of stLFR. In some embodiments, the target DNA fragments are transposed with transposons having particular positional barcodes that can be used to order sequence reads.
Provided is a repeated sequencing method, comprising: on the basis of a first sequencing primer, performing first sequencing on a sample to obtain a first sequencing sequence based on a first sequencing synthetic strand; bringing an elution reagent into contact with the first sequencing synthetic strand so as to remove the first sequencing synthetic strand; on the basis of the first sequencing primer, performing second sequencing on the sample to obtain a second sequencing sequence based on a second sequencing synthetic strand; and comparing the first sequencing sequence with the second sequencing sequence to determine sequence information of the sample.
A biochemical substance analysis system is used to detect biological characteristics of a sample in a flow cell, and includes a detection system, a scheduling system, a biochemical reaction system, and a control system. The scheduling system is used to schedule the flow cell at different sites, including sites in the detection system and sites in the biochemical reaction system. The biochemical reaction system is used to allow the sample to react in the flow cell. The detection system is used to detect a signal from the reacted sample to obtain a signal representing the biological characteristics of the sample. The control system is used to control the detection system, the scheduling system, and the biochemical reaction system to cooperate. The present disclosure improves the automation degree and flux of the biochemical substance analysis.
G01N 21/63 - Systèmes dans lesquels le matériau analysé est excité de façon à ce qu'il émette de la lumière ou qu'il produise un changement de la longueur d'onde de la lumière incidente excité optiquement
Provided in the present invention is a multiplex RT-PCR amplification method. Primers are designed to be completely or partially complementary hairpin structures, so that the 3'-ends of the primers lose the binding and extension capabilities during an RT process; in addition, the 5'-end strands of the primers comprise one or more cleavage sites at which the 5'-end strands are cleaved by a specific cleavage agent, so that during the RT process, the 5'-end strands of the primers are cleaved at the cleavage sites by means of the specific cleavage agent, but the complementary double strands are maintained. During a high-temperature process of PCR, the cleaved 5'-end strands are free so as to release 3'-end strands to serve as primers for target amplification, thereby regaining the capability of binding primers to templates and the extension capability of 3'-ends.
The present invention provides an oligonucleotide combination, comprising specific oligonucleotides and universal primers. A long-chain primer comprising a universal primer sequence and a sequence complementary to the specific oligonucleotides is generated by means of the specific oligonucleotides and the universal primers. The long-chain primer realizes enrichment of a target area and addition of a universal sequence on a product in a PCR process, so that one-step construction of a targeted library is realized.
The present invention relates to the field of nucleic acid sequencing. In particular, the present invention relates to a scanning reagent containing a saponin compound, a kit containing the scanning reagent and a method for nucleic acid sequencing by means of using the scanning reagent.
C07J 17/00 - Stéroïdes normaux contenant du carbone, de l'hydrogène, un halogène, ou de l'oxygène, ayant un hétérocycle contenant de l'oxygène non condensé avec le squelette du cyclopenta[a]hydrophénanthrène
Provided in the present disclosure are a method and apparatus for performing joint-calling acceleration on high-performance sequencing data, and an electronic device and a storage medium. The method comprises: determining a division mode for genome variation data according to population information of a plurality of samples; dividing the genome variation data according to the division mode, so as to obtain a plurality of variation data segments corresponding to each piece of genome variation data; merging variation data segments, which have the same rank, in the genome variation data, so as to obtain merged variation data; performing population variation detection on each piece of merged variation data, so as to obtain population genome variation data of each piece of merged variation data; and merging the population genome variation data, so as to obtain target population genome variation data of the plurality of samples. Thus, during population variation detection, by means of performing population anomaly detection on a plurality of pieces of local merged data, and merging detection results, the efficiency of acquiring complete population genome variation data can be improved.
The present disclosure relates to a method for analyzing the sequence of a target polynucleotide by polymerizing a nucleotide mixture and a polymerase multiple times to achieve full polymerization, thereby satisfying the requirements of sequencing, and improving read length and accuracy. Further, the present disclosure also relates to a reagent test kit, the reagent test kit being used for analyzing or sequencing polynucleotides.
The present disclosure provides a population variation detection method and apparatus, an electronic device, and a storage medium. The method comprises: according to population information of a plurality of samples, determining a division mode of genome variation data; according to the division mode, dividing the genome variation data to obtain a plurality of variation data fragments corresponding to the genome variation data; combining variation data fragments in the genome variation data having the same ranking to obtain combined variation data; respectively performing population variation detection on the combined variation data to obtain population genome variation data of the combined variation data; and combining the population genome variation data to obtain target population genome variation data of the plurality of samples. Therefore, in the population variation detection process, population variation detection is respectively performed on a plurality pieces of local combined data, and detection results are combined, so that the efficiency of acquiring complete population genome variation data can be improved.
The present invention relates to the field of biological information, and provides an acceleration method and apparatus for genetic testing and an electronic device. The method of the present invention mainly comprises: obtaining a first variation data set and a second variation data set according to a first genetic sample set; and obtaining a variation quality control model according to the second variation data set, and processing the first variation data set according to the variation quality control model to obtain a first variation detection result corresponding to the first variation data set. According to the technical solution of the present application, variation detection data is divided into a first variation data set and a second variation data set by means of temporary interval information, so that processing is directly carried out with respect to intervals during subsequent quality control, thereby achieving the effect accelerating computation. Compared with a mode of dividing data and allocating tasks during quality control in the past, the method reduces testing time consumption, and effectively improves the computational efficiency of population genome analysis.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Genetic testing apparatus for medical purposes; Apparatus for DNA and RNA testing for medical purposes; Medical apparatus and instruments for use in medical diagnostics for detecting genetic abnormalities and disease; Apparatus for use in medical analysis; Testing apparatus for medical purposes; Diagnostic apparatus for medical purposes used in medical laboratories; Apparatus for the regeneration of stem cells for medical purposes; Blood testing equipment; Medical apparatus and instruments; Sample and library preparation devices for medical diagnosis, testing, and treatment purposes; Analyzers for bacterial identification for medical purposes. Medical screening; Medical analysis services for diagnostic and treatment purposes provided by medical laboratories; Medical assistance; Genetic testing for medical purposes; Medical analysis services for the diagnosis of cancer; Performing diagnosis of diseases; Providing medical information from a web site; Telemedicine services; Medical services; Remote monitoring of medical data for medical diagnosis and treatment; Genetic testing for cancer risk for medical purposes; Genetic testing of animals for diagnostic or treatment purposes.
Medical apparatus and instruments; needles for medical
purposes; blood collection needles; capillary tubes for
blood; apparatus for taking blood; apparatus for taking
blood samples.
17.
METHOD FOR ADJUSTING VOLUME OF NUCLEIC ACID NANOSPHERES AND USE THEREOF
The present invention belongs to the field of nucleic acid sequencing, and specifically relates to a method for adjusting the volume of nucleic acid nanospheres and the use thereof. Provided in the present invention is a method for adjusting the volume of nucleic acid nanospheres. The method comprises performing condensation treatment on nucleic acids (e.g., adding a nucleic acid condensing agent and/or adjusting the pH value to 3-4.5, so that positively charged counter ions are gathered around the nucleic acids, and negative charges on the surface of the nucleic acids are neutralized by cations by mean of an electrostatic combining effect between anions and cations, thereby causing condensation of the nucleic acids during morphology), which can further alter the morphology of the nucleic acid nanospheres, adjust the volume of the nucleic acid nanospheres, and compress the nucleic acid nanospheres. The nucleic acid nanospheres obtained by means of the method (performing condensation treatment on nucleic acid nanospheres) are more compact, smaller in terms of morphology, and more uniform in terms of size. The method can be used for any situation where the volume of nucleic acid nanospheres needs to be adjusted (reduced) (e.g., a loading process of nucleic acid nanospheres (such as DNB), and a sequencing process after loading).
Provided is a DNA polymerase mutant for sequencing. The DNA polymerase mutant comprises: compared with a Pyrococcus abyssi DNA polymerase exo-mutant, at least three amino acid mutations in the following four sites or functionally equivalent sites: position 409, position 410, position 411, and position 486. The Pyrococcus abyssi DNA polymerase exo-mutant has an amino acid sequence as shown in SEQ ID NO: 1.
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
19.
METHOD FOR IMPROVING LOADING EFFICIENCY AND QUALITY OF NUCLEIC ACID NANOBALLS
A method for adjusting the volume of nucleic acid nanoballs. By performing coagulation treatment on a nucleic acid (for example, adding a nucleic acid coagulating agent and/or adjusting the pH value to 3-4.5, so that positively charged counter ions are gathered around the nucleic acid, and negative charges on the surface of the nucleic acid are neutralized by cations by means of electrostatic bonding of anions and cations, thereby enabling the nucleic acid to coagulate), the shape of the nucleic acid nanoballs can be changed, the volume of the nucleic acid nanoballs can be adjusted, and the nucleic acid nanoballs can be compressed. The nucleic acid nanoballs obtained by the method (performing coagulation treatment on the nucleic acid nanoballs) are more compact, smaller in shape, and more uniform in size. The method can be used in any situation where the volume of the nucleic acid nanoballs needs to be adjusted (reduced) (such as the loading process of nucleic acid nanoballs (such as DNBs), and the sequencing process after loading).
C40B 50/18 - Synthèse en phase solide, c.-à-d. dans laquelle au moins un bloc servant à créer la bibliothèque est lié à un support solide au cours de la création de la bibliothèqueProcédés particuliers de clivage à partir du support solide utilisant un procédé particulier d'ancrage au support solide
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
20.
FLUORESCENT DYE, SYNTHESIS THEREFOR AND USE THEREOF
The present invention relates to a dye compound and a use thereof as a fluorescent marker, and also relates to a method for preparing the compound, a nucleotide or oligonucleotide labeled by the compound, and a nucleic acid sequencing method.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
21.
METHOD FOR PHOSPHORYLATION OF NUCLEIC ACID AT 3' END AND LIBRARY CONSTRUCTION
Provided is a method for phosphorylation of a nucleic acid at the 3' end and library construction. The library construction method comprises: performing phosphorylation of a nucleic acid fragment at the 3' end; ligating the nucleic acid fragment having phosphorylation at the 3' end to a linker under the action of an RtcB ligase, so as to ligate the 3' end of the nucleic acid fragment to the 5' end of the linker; and amplifying the ligated product, so as to obtain a sequencing library.
The application discloses a flow cell system which includes a flow cell. The flow cell includes a substrate that is configured to support an analyte array, a cover, and an adhesive arrangement spacing the cover from the substate to define a fluid gap between the substrate and cover. The flow cell also includes an inlet in fluid communication with the fluid gap and an outer perimeter. In one example implementation, fluid enters the flow cell at the inlet, flows through the fluid gap, and exits the flow cell at the outer perimeter between the substrate and the cover, with adhesive gaps in the adhesive arrangement at the outer perimeter of the flow cell allowing fluid to exit from the fluid gap.
The application discloses a flow cell system which includes a flow cell. The flow cell includes a substrate that is configured to support an analyte array, a cover, and an adhesive arrangement spacing the cover from the substate to define a fluid gap between the substrate and cover. The flow cell also includes an inlet in fluid communication with the fluid gap and an outer perimeter. In one example implementation, fluid enters the flow cell at the inlet, flows through the fluid gap, and exits the flow cell at the outer perimeter between the substrate and the cover, with adhesive gaps in the adhesive arrangement at the outer perimeter of the flow cell allowing fluid to exit from the fluid gap.
Variable-gap flow cells, and systems and methods using variable-gap flow cells. In one example, the variable-gap flow cell includes a substrate and a cover movably spaced from one another to define a variable fluid gap between them. An actuator may move one of the substrate and cover between a dispensing configuration and a processing configuration. In the dispensing configuration, the substrate and cover are spaced more widely apart such that a volume of reagent dispensed into the fluid gap is substantially less than the volume of the fluid gap. In the processing configuration, the substrate or cover has been moved by the actuator to narrow the fluid gap, such that the dispensed reagent spreads to substantially fill the narrowed fluid gap. In some implementations, variable-gap flow cells enable scaling of the flow cell for larger substrates while minimizing reagent consumption.
The present invention relates to a method for analyzing a sequence of a target polynucleotide. Full polymerization is achieved by means of multiple times of polymerization of a nucleotide mixture and polymerase, and a marker is detected while the polymerization reaction is performed. Furthermore, the present invention relates to a kit, capable of being used for analyzing or sequencing a polynucleotide.
Disclosed in the present invention are a slide and a biochemical analysis device. The slide comprises: a housing, wherein at least one flow channel is provided in the housing, and is provided with a liquid inlet; and a main liquid inlet, which is provided in the housing, wherein liquid inlets of a plurality of flow channels are all in communication with the main liquid inlet. In the present application, after the slide is suctioned to a holder, the slide can be in communication with a liquid inlet of the holder only by means of the main liquid inlet; therefore, only the main liquid inlet and the liquid inlet of the holder need to be sealed, but the liquid inlet of each flow channel does not need to be independently sealed. The sealing effect is effectively guaranteed, the sealing convenience is guaranteed, and the compatibility range of the slide is expanded, that is, slides with different flow channels can adapt to the range of different run data.
A slide glass, a sequencer, and a biochemical analysis apparatus. The slide glass comprises: a shell, wherein at least one flow channel is formed in the shell, and the flow channel is provided with a liquid inlet; and a main liquid inlet formed on the shell, wherein the liquid inlets of a plurality of flow channels are all communicated with the main liquid inlet. After the slide glass is suctioned to a carrier stage, only the main liquid inlet is communicated with the liquid inlet of the carrier stage, and therefore, it is only needed to seal the main liquid inlet and the liquid inlet of the carrier stage, and it is not needed to separately seal the liquid inlet of each flow channel. The sealing effect is effectively guaranteed, the sealing convenience is guaranteed, and the compatibility range of the slide glass is expanded, that is, the slide glasses of different flow channels can adapt to different ranges of the amount of once loaded data.
Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.
The present invention relates to the technical field of library construction, and particularly relates to a method for efficiently and quickly constructing a nucleic acid library. The present invention provides an adaptor composition. The adaptor composition can simultaneously attach a 5' end adaptor and a 3' end adaptor to a single-stranded template nucleic acid in a same reaction system by means of the 5' end adaptor, a first ligase, the 3' end adaptor, and a second ligase, without the need to add the 5' end adaptor and the 3' end adaptor in separate steps, thereby greatly reducing the reaction steps and the library construction time, and improving the adaptor attachment efficiency; moreover, the adaptor attachment directionality can be guaranteed, the template nucleic acid loss is reduced, and the utilization rate of original template nucleic acids is increased; no additional fixed sequence is added onto the template, the sequencing quality is not affected, there is no need to perform additional truncation by a certain length of a sequence requiring sequencing, and adaptors will not be attached to each other.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
A nucleic acid tester. The nucleic acid tester comprises: a shell (1) internally provided with an accommodating space, wherein a sample mounting site (16) is arranged in the accommodating space; a control device, which is arranged in the accommodating space; at least one set of optical test assemblies (3), which is arranged in the accommodating space, connected to the control device, and configured to emit test light to the sample mounting site (16) and/or configured to receive excitation light returned by a target in a sample at the sample mounting site (16); and an indication element (4), which is arranged at the shell (1), connected to the control device, and configured to indicate a test result of whether the target is contained in the sample.
G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
A sequencing method, and a sequencing method and system. The sequencing method comprises: (1) reacting a target nucleic acid immobilized on the surface of a chip with a polymerization reagent, and incorporating a nucleotide or a nucleotide analog to obtain a reaction product; (2) detecting fluorescent signals; (3) reacting the reaction product with a regeneration reagent to obtain a product that can be used for the next round of polymerization reaction; (4) repeating step (1) to step (3); and continuing in this manner for multiple rounds of cycles to finally obtain sequencing data, wherein the reaction time in at least one of step (1) and step (3) is calculated by a predetermined cycle number-reaction time relationship.
Provides are variant family A polymerases that incorporate 3′-blocked nucleotides into a DNA extension product, a kit comprising such polymerases and methods of using the polymerases in DNA extension reactions, e.g., sequencing reactions.
The methods and compositions disclosed herein relate to preparing libraries to sequence long molecules comprising repetitive regions. In some embodiments, this strategy involves substituting selected nucleotides in each molecule to predetermined, different nucleotides at low frequencies in random positions and co-barcoding the molecule in single-tube LFR reactions to generate mutagenized barcoded fragments. Each mutagenized barcoded fragment comprises a barcode, and the barcode comprises a barcode sequence.
A thermostable strand displacement polymerase and the use thereof in sequencing. Compared with a wild-type Bst DNA polymerase large fragment, the provided mutant Bst DNA polymerase large fragment has more than one mutated amino acid residue. The positions of the mutated amino acid residues relative to the wild-type Bst DNA polymerase large fragment include position 81 and/or position 99 and/or position 321 and/or position 323 and/or position 336 and/or position 400 and/or position 429 and/or position 486 and/or position 488 and/or position 496 and/or position 534 and/or position 547 and/or position 556. The mutant Bst DNA polymerase large fragment has a stable catalytic activity at 65°C, and has a higher strand displacement DNA polymerase activity relative to the wild-type Bst DNA polymerase large fragment.
This application relates to methods and compositions for preparing a library of polynucleotides for sequencing comprises in a single reaction mixture. The method comprises contacting a double-stranded target nucleic acid with one or more nicking agents to produce overlapping nucleic acid fragments separated by staggered single-stranded breaks; and contacting a partially double-stranded first adapter with at least one of the nucleic acid fragments in the presence of a ligase, thereby ligating the 5′ terminus of the double-stranded region of the first adapter to the 3′ terminus of the at least one of the nucleic acid fragments using a DNA ligase via 3′ branch ligation. The first adapter comprises (i) a double-stranded blunt end having a 5′ terminus and a 3′ terminus and (ii) a single-stranded region comprising a barcode.
Provided are a nucleic acid amplification method, a single-stranded multi-copy DNA concatemer and the use thereof. The method comprises: linking a nucleic acid with a known sequence to obtain a nucleic acid to be amplified, and performing an amplification ligation reaction using a primer pair designed on the basis of the known sequence contained in the nucleic acid to be amplified, thereby obtaining a first double-stranded multi-copy DNA concatemer, wherein the two primers of the primer pair can comprise different enzyme cleavage sites 1 and 2 respectively; using an enzyme 1 to cleave the enzyme cleavage site 1 contained in the double-stranded multi-copy DNA concatemer so as to form a single-stranded gap in the double-stranded DNA loop, and using a digestive enzyme to digest the single-stranded gap, so as to obtain a first single-stranded multi-copy DNA concatemer; performing an amplification ligation reaction using a primer with the first single-stranded multi-copy DNA concatemer serving as a template so as to obtain a second double-stranded multi-copy DNA concatemer, using an enzyme 2 to cleave the enzyme cleavage site 2 contained in the second double-stranded multi-copy DNA concatemer so as to form a gap single strand in the second double-stranded multi-copy DNA concatemer, and using the digestive enzyme to digest the gap single strand, so as to obtain a second single-stranded multi-copy DNA concatemer; and using the above-mentioned single-stranded multi-copy DNA concatemer for nucleic acid sequencing .
A gene data encoding method and apparatus, a gene data decoding method and apparatus, and a genetic testing system. A plurality of carrying points are provided on a gene slide, and the carrying points support a sample to be detected having a gene sequence. The encoding method comprises: irradiating a gene slide with excitation light, exciting a sample to be detected so as to generate a fluorescence signal, and generating a reflected light signal at at least one associated position of each carrying point; acquiring the fluorescence signal and the reflected light signal, comparing the fluorescence signal with a first signal threshold and performing encoding according to a comparison result to generate a first identifier, and comparing the reflected light signal with a second signal threshold and generating a second identifier according to a comparison result; and on the basis of the first identifier and the second identifier, generating an encoding sequence associated with a gene sequence. In the process, redundant signal processing is not required, thereby avoiding the large workload of data processing caused by traditional ADC acquisition, making the total amount of data small, saving the storage space, increasing the data utilization rate, and reducing the cost.
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Medical laboratory services; genetic testing for scientific
research purposes; structural and functional analysis of
genomes; genetic screening for scientific research purposes;
blood analysis services; DNA screening for scientific
research purposes.
39.
GENE SEQUENCING DEVICE, GENE SEQUENCING METHOD, AND NUCLEIC ACID TEST METHOD
Provided are a gene sequencing device and a gene sequencing method. The gene sequencing device comprises: a biosensing layer comprising one or more sample receiving sites and one or more photosensitive circuits having one-to-one correspondence with the one or more sample receiving sites, wherein the sample receiving sites are configured to receive samples, the photosensitive circuits are configured to receive fluorescent signals emitted by the samples at the corresponding sample receiving sites and to generate analog electrical signals according to the fluorescent signals, and each photosensitive circuit comprises a quantum image sensor; a circuit structure layer, comprising an analog-to-digital conversion circuit configured to convert the analog electric signals into digital electric signals; and a logic processing layer configured to determine the characteristics of the fluorescent signals according to the digital electric signals and to determine the types and/or sequences of bases contained in the samples according to the characteristics of the fluorescent signals.
The present application discloses a leveling control method for a chip, and a related device. The leveling control method comprises: a preparation step: starting a focusing apparatus, and adjusting the distance between the focusing apparatus and a chip such that the focusing apparatus receives an optical signal of a sample to be detected; a detection step: driving the relative movement between the focusing apparatus and a motion platform, such that the focusing apparatus respectively performs focusing on at least three detection points of the chip, and respectively acquiring a focusing adjustment distance between each detection point and the focusing apparatus along the direction of an optical axis of the focusing apparatus; and a leveling step: calculating a deviation angle of the chip on the basis of focusing adjustment distances and the distance between every two detection points respectively, and the motion platform leveling the chip on the basis of the deviation angle. According to the present application, an optical detection platform is automatically leveled and focused by means of the motion platform, thereby reducing the amount of manual labor and improving the efficiency and accuracy.
H01L 21/683 - Appareils spécialement adaptés pour la manipulation des dispositifs à semi-conducteurs ou des dispositifs électriques à l'état solide pendant leur fabrication ou leur traitementAppareils spécialement adaptés pour la manipulation des plaquettes pendant la fabrication ou le traitement des dispositifs à semi-conducteurs ou des dispositifs électriques à l'état solide ou de leurs composants pour le maintien ou la préhension
G02B 21/26 - PlatinesMoyens de réglage pour celles-ci
Provided are a recombinant protein having improved amplification performance, a nucleic acid encoding the recombinant protein, a vector comprising the nucleic acid, a kit comprising the protein, the nucleic acid or the vector, a method for preparing the recombinant protein, and a method for amplifying a target DNA by using the recombinant protein. As a chimeric family A DNA polymerase, the recombinant protein has improved processivity, the amplification performance of the recombinant protein is remarkably better than that of a wild-type family A DNA polymerase, and the thermal stability thereof is higher than that of a family B DNA polymerase.
Embodiments of the disclosure include methods and systems for nucleic acid sequencing that may include an objective coupled to an actuator, wherein the actuator is configured to move the objective over a surface of a substrate. In some embodiments, a droplet may be disposed on the surface of the substrate, and the droplet may be moved along with the objective. The distal end of the objective may include a material that provides a higher friction against the droplet than a material of the surface of the substrate. In some embodiments, the distal end of the objective may be immersed in a fluid as it is moved over the surface of the substrate. The substrate may include vertical walls within a region to retain the fluid.
The present application discloses a liquid path system and a related device thereof, and a cleaning method. The liquid path system comprises: a reagent needle assembly; a cavity structure capable of moving relative to the reagent needle assembly so as to be in contact or non-contact with the reagent needle assembly; a flow cell having one end directly communicated with the internal cavity of the reagent needle assembly or communicated with the internal cavity of the reagent needle assembly by means of a pipeline; and a fluid power member directly communicated with the other end of the flow cell or communicated with the other end of the flow cell by means of a pipeline, wherein the fluid power member is used for delivering a fluid in the flow cell into the cavity structure or delivering a fluid in the cavity structure into the flow cell, and is used for pumping a fluid in a reagent needle into the flow cell when the reagent needle assembly is in non-contact with the cavity structure. According to the present application, the whole liquid path system can be efficiently and thoroughly cleaned.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B08B 9/032 - Nettoyage des surfaces intérieuresÉlimination des bouchons par l'action mécanique d'un fluide en mouvement, p. ex. par effet de chasse d'eau
44.
SENSING SYSTEM, CHIP PACKAGE STRUCTURE, SEQUENCING SLIDE, AND SEQUENCING METHOD
Disclosed in the present application are a sensing system, a chip package structure, a sequencing slide, and a sequencing method. The sensing system comprises at least two sensing substrates each having a sensing site array, the at least two sensing substrates being spaced apart from each other and stacked. In the stacking direction of the sensing substrates, the sensing site arrays of the two adjacent sensing substrates are arranged opposite to each other and spaced apart from each other so as to form a fluid channel, the sensing site arrays both being exposed to the fluid channel. Each sensing substrate comprises a sensing layer close to the fluid channel and a sensor layer stacked on the side of the sensing layer away from the fluid channel, the sensing site array being arranged on the sensing layer. The sensor layers are provided with sensors for acquiring sensing signals at sensing sites. The sensing system further comprises a signal transmission structure, and the signal transmission structure is electrically connected to the sensor layers of both the two adjacent sensing substrates in the stacking direction, and transmits the sensing signals to the outside of the sensing system.
Provided is a synchronous sequencing method, comprising: constructing a sequencing library for a nucleic acid sample under test; loading the sequencing library to a sequencing chip; performing multiple rounds of synchronous sequencing on the sequencing library, wherein a set of images formed by each round of sequencing reaction is an original image set for synchronous sequencing; on the basis of the original image set for synchronous sequencing, acquiring a base interpretation result of synchronous sequencing; correcting the numerical value of the signal strength of each base channel on the basis of predetermined correction parameters to obtain a corrected base interpretation result; and on the basis of the corrected base interpretation result, determining a base result output by the synchronous sequencing.
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
46.
GENE SEQUENCING METHOD AND SYSTEM, AND GENE IMAGE ACQUISITION METHOD AND DEVICE
The present invention relates to a gene sequencing method and system, and a gene image acquisition method and device. The gene image acquisition method comprises: acquiring a gene image obtained by a camera photographing a sample to be sequenced; determining whether image features of the gene image satisfy a preset feature condition; if no, determining target parameter values of photographing parameters matching a current photographing scene; and triggering the camera to re-photograph said sample according to the target parameter values, such that image features of a re-photographed gene image satisfy the preset feature condition. Therefore, gene images satisfying gene sequencing requirements can be provided for gene sequencing, thereby achieving effective gene sequencing and improving the overall efficiency and accuracy of gene sequencing.
The present invention provides a multi-template nucleic acid synchronous sequencing method and use thereof. The method comprises: providing a plurality of composite template sample points arranged with a plurality of sequencing templates; hybridizing the plurality of sequencing templates with corresponding sequencing primers; conducting, on the basis of the sequencing primers, a plurality of sequencing reaction runs on the plurality of sequencing templates hybridized with the sequencing primers, wherein in each sequencing reaction run, signal intensities produced by the plurality of sequencing templates are different; and classifying the signals of the sequencing channels among the plurality of sequencing templates on the basis of the difference of the signal intensities for each of the plurality of sequencing reaction runs.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
48.
TARGETED METHYLATION LIBRARY CONSTRUCTION SYSTEM BASED ON MULTIPLEX PCR, METHOD, AND USE THEREOF
The present invention provides a targeted methylation library construction system based on multiplex PCR, a method, and use thereof. The method comprises: a first amplification reaction run 1, wherein the system of the first amplification reaction run 1 comprises: a template DNA and one or more primer pairs, each primer pair comprises a forward primer and a reverse primer, and the 5' end of the forward primer or the reverse primer in each primer pair is provided with a universal sequence 1; and a second amplification reaction run 1, wherein the system of the second amplification reaction run 1 comprises a product of the first amplification reaction run 1 and one or more primer sets, and each primer set comprises: 1) a semi-nested primer 1 with a 3' end linked to one or more target regions in the product of the first amplification reaction run 1 and a 5' end having a universal sequence 2, and 2) a universal primer 1 with a 3' end identical to that of the universal sequence 1 and/or a universal primer 2 with a 3' end identical to that of the universal sequence 2.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
49.
METHOD FOR REDUCING ERROR RATE IN SYNCHRONOUS PAIRED-END SEQUENCING
Provided is a method for reducing the error rate in synchronous paired-end sequencing, comprising: generating a first strand sequencing template and a second strand sequencing template by means of rolling circle amplification and multiple displacement amplification with the circular DNA molecule of interest as the template; and separately or simultaneously hybridizing a first strand sequencing primer and a second strand sequencing primer with the first strand sequencing template and second strand sequencing template to simultaneously conduct sequencing on the first strand and the second strand, so as to give the sequences of the sense and antisense strands of the DNA. Two dNTP monomers with different modifications are used: the first dNTP monomer is introduced with fluorescent groups indirectly through non-covalent bonds, and the second dNTP monomer is introduced with fluorescent groups directly through covalent bonds. Both the first dNTP monomer and/or the second dNTP monomer contain reversible blocking groups. The changes in the linkage or position of the fluorescent labels minimize the quenching effect among dNTPs, thereby improving the sequencing accuracy.
Disclosed in the present application are a focusing control method and a related apparatus. The focusing control method is used for a focusing platform system, which comprises a focusing device, a micro-motion platform and a macro-motion platform, the focusing device being mounted on the micro-motion platform, and the macro-motion platform carrying a test sample, or, the micro-motion platform being mounted on the macro-motion platform, and the micro-motion platform carrying the test sample. The focusing control method comprises: starting the focusing device; obtaining a first target displacement of movement of the macro-motion platform, and driving the macro-motion platform to move to a first target position; and obtaining a second target displacement of movement of the micro-motion platform, and driving the micro-motion platform to move to a second target position. In the present application, by means of large-stroke rapid adjustment of the macro-motion platform for a focusing position, combined with high-precision adjustment of the micro-motion platform, a highly stable, rapid, large-stroke, high-precision optical focusing positioning movement is implemented.
Provided is a double-end cyclization primer which comprises a first nucleic acid single-stranded molecule and a second nucleic acid single-stranded molecule. The 5' end of the first nucleic acid single-stranded molecule is connected to the 5' end of the second nucleic acid single-stranded molecule by means of an adapter. The cyclization primer obtained by connecting the first nucleic acid single-stranded molecule to the second nucleic acid single-stranded molecule by means of the adapter can be complementarily paired with the sequences of the sense and antisense strand tail end adapter parts of a double-stranded nucleic acid template molecule at the same time; a cyclization reaction is carried out; after rolling circle amplification, a sense strand nucleic acid nanosphere and an antisense strand nucleic acid nanosphere can be obtained at the same time, and then the sense and antisense strands can be sequenced; multiple strand displacement amplification reactions are not needed, so that the steps of hybridization, unwinding, and template removal and the like can be omitted, thereby reducing the time for subsequent sequencing.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
C40B 50/18 - Synthèse en phase solide, c.-à-d. dans laquelle au moins un bloc servant à créer la bibliothèque est lié à un support solide au cours de la création de la bibliothèqueProcédés particuliers de clivage à partir du support solide utilisant un procédé particulier d'ancrage au support solide
52.
MANIFOLD ASSEMBLY, FLUID SYSTEM AND BIOCHEMICAL ANALYSIS AND DETECTION PLATFORM
A manifold assembly, a fluid system and a biochemical analysis and detection platform. The manifold assembly comprises: a manifold body (101), which has one or more first external flow channel interfaces (102-109), one or more first internal flow channel interfaces (127-134), one or more second internal flow channel interfaces (120, 123, 125, 126, 135-138), one or more second external flow channel interfaces (118) and a plurality of internal flow channels (162-179), wherein the plurality of internal flow channels comprise one or more first internal flow channels (162-169) and one or more second internal flow channels (170-179), each first external flow channel interface (102-109) is in communication with at least one of the first internal flow channel interfaces (127-134) by means of the corresponding first internal flow channel (162-179), and each second external flow channel interface (118) is in communication with at least one of the second internal flow channel interfaces (120, 123, 125, 126, 135-138) by means of the corresponding second internal flow channel (170-179); and one or more internal flow channel control valves (110-117), wherein each internal flow channel control valve (110-117) is connected between at least one of the first internal flow channel interfaces (127-134) and at least one of the second internal flow channel interfaces (120, 123, 125, 126, 135-138), and is configured to control connection and disconnection between the first internal flow channel interfaces (127-134) and the second internal flow channel interfaces (120, 123, 125, 126, 135-138) connected thereto.
C02F 1/40 - Dispositifs pour séparer ou enlever les substances grasses ou huileuses, ou les matières flottantes similaires
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
53.
METHOD FOR RAPIDLY PREPARING MULTIPLEX PCR SEQUENCING LIBRARY AND USE THEREOF
A method for rapidly preparing a multiplex PCR sequencing library and use thereof. The oligonucleotide combination comprises a specific oligonucleotide and a universal primer. The 3' end of the specific oligonucleotide is defined as a universal sequence, and the 5' end is defined as a specific sequence. The universal sequence located at the 3 end of the specific oligonucleotide is complementary to the 3' end of the universal primer. The 3' end of the universal primer extends under the action of a polymerase, and a product with the 5' end comprising the universal sequence and the 3' end comprising the specific sequence is obtained. The 3' end of the product can be complementary to a target region to be determined. The target region is amplified under the action of a polymerase, and the amplification yields a product comprising the universal sequence and the target sequence to be determined. As the 3' end is a single fixed sequence, formation of complementary structures and generation of dimers can be effectively prevented.
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
54.
SYNTHESIS METHOD FOR SECOND STRAND IN DNB PAIRED-END SEQUENCING, SEQUENCING METHOD, AND RELATED PRODUCTS
Provided are a synthesis method for a second strand in DNB paired-end sequencing, a sequencing method, and related products. The synthesis method for the second strand comprises: after DNB is subjected to first-strand sequencing, using a second-strand synthesis primer to synthesize the second strand, wherein the second-strand synthesis primer is a primer capable of being anchored on the surface of a sequencing chip. Using the second-strand synthesis primer that is anchored on the surface of a sequencing vector for the synthesis of the second strand makes the process of synthesizing the second strand relatively more stable. Moreover, the synthesized second-strand template is anchored on the sequencing vector, and subsequent sequencing of the second-strand template strand that is anchored on the sequencing vector can achieve a relatively higher quality of the second-strand sequencing.
Provided are an in-situ sequencing method, and a method for performing area division on an in-situ sequencing result. The in-situ sequencing method comprises: performing in-situ transcriptome library building on a sample, wherein each nucleic acid molecule of an obtained sequencing library carries a coordinate label and a UMI label; performing sequencing to obtain a plurality of sequencing reads, involving: on the basis of comparison, determining additional information of at least some of the plurality of sequencing reads, wherein the additional information comprises at least one of the following: corresponding genes, coordinates, UMIs and classification results (whether belonging to mitochondrial/nuclear conservative long non-coding/mature/immature/sheared/unsheared RNA molecules) of the sequencing reads; classifying the sequencing reads into a first sequencing read group and a second sequencing read group; acquiring a plurality of first high-density areas of the first sequencing read group and a plurality of second high-density areas of the second sequencing read group; and combining the plurality of first high-density areas with the plurality of second high-density areas, so as to construct a pseudo-cell area.
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 35/00 - TIC spécialement adaptées aux bibliothèques combinatoires in silico d’acides nucléiques, de protéines ou de peptides
G06N 5/00 - Agencements informatiques utilisant des modèles fondés sur la connaissance
56.
FLUID SYSTEM, BIOCHEMICAL ANALYSIS AND TEST PLATFORM, AND FLUID OPERATION METHOD
A fluid system, a biochemical analysis and test platform, and a fluid operation method. The fluid system comprises: one or more first main flow paths (L6) which are configured to connect reagent storage chambers; a second main flow path (L1); a reaction flow path, comprising a flow cell (C1); a bypass flow path (L5) connected in parallel to the reaction flow path; a third main flow path (L4); one or more branch flow paths; a first turning member (T1), which is connected to the one or more first main flow paths (L6), the second main flow path (L1) and the at least one branch flow path, such that any two flow paths of different types among the connected flow paths communicate with each other while the remaining flow paths are disconnected; a second turning member (T2), which is connected to the second main flow path (L1), the reaction flow path and the bypass flow path (L5), such that any two flow paths among the connected flow paths communicate with each other while the remaining flow paths are disconnected; and a third turning member (T3), which is connected to the third main flow path (L4), the reaction flow path and the bypass flow path (L5), such that any two flow paths among the connected flow paths communicate with each other while the remaining flow paths are disconnected.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
57.
REAGENT TEST KIT AND APPLICATION THEREOF IN SEQUENCING
A reagent test kit and an application thereof in sequencing. The reagent test kit comprises: a first reagent and a second reagent, the first reagent being selected from a disulfide bond reducing agent; and the second reagent being selected from a blocking reagent suited to blocking a disulfide bond.
A gene sequencing chip and a package structure thereof, a cleaning method, a production method, and a system. The gene sequencing chip comprises at least one chip body. The chip body comprises: a substrate layer provided with a circuit; at least one electrode layer located on the substrate layer; and at least one protective layer located on the substrate layer and used for keeping the insulativity between the substrate layer and the outside. The protective layer and the electrode layer are not located on a same plane, at least part of the electrode layer is exposed out of the protective layer, and the portion of the electrode layer exposed out of the protective layer comprises a plurality of electrode parts, wherein the plurality of electrode parts are arranged in an array, and DNBs are loaded to the electrode parts to form a sequencing area. Instead of using existing design of molecular hierarchical modification, the gene sequencing chip can be produced using only a conventional semiconductor manufacturing process and on the basis of a conventional semiconductor material; in cooperation with a protective layer, arrayed sites formed by the electrode layer are resistant to corrosion of a biochemical reagent, the structure is more suitable for adsorption and binding of DNA, and the morphology and material still keep original characteristics after repeated use; and the gene sequencing chip can be repeatedly used.
A method and device for magnetic bead detection, and a magnetic bead detection optical system. The method comprises: using a laser to irradiate magnetic bead samples to be detected, such that magnetic beads are excited to generate fluorescence signals; illuminating the magnetic bead samples on the basis of a contour light source to generate contour optical signals of the magnetic beads; acquiring first optical image information corresponding to the fluorescence signals; acquiring second optical image information corresponding to the contour optical signals; and combining the first optical image information and the second optical image information to determine the types and concentration distribution of the magnetic beads. By means of obtaining the fluorescence signal and the contour optical signal of a sample to be detected so as to image and identify the types and concentrations of the protein, the detection of protein samples to be detected having different concentrations and types can be achieved, thereby increasing the detection efficiency of the samples.
Provided is a system for maintaining the vacuum constant, which comprises a negative pressure adsorption object, an adsorption supporting member, fluid power devices (4), and a fluid control valve (3). The adsorption supporting member is configured for supporting the negative pressure adsorption object. Each fluid power device (4) is communicated with the adsorption supporting member by means of a pipeline and is configured for vacuumizing the adsorption supporting member so as to adsorb the negative pressure adsorption object. The fluid control valve (3) is arranged on the pipeline where each fluid power device (4) is communicated with the adsorption supporting member, and is configured for switching or increasing the fluid power devices for vacuumizing. At least one fluid control valve (3) is arranged. At least two fluid power devices (4) are arranged. According to the system for maintaining the vacuum constant, the error probability of a detection task caused by a failure of the fluid power devices is reduced. A method for maintaining the vacuum constant and a gene sequencer are further provided.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B25B 11/00 - Porte-pièces ou dispositifs de mise en position non couverts par l'un des groupes , p. ex. porte-pièces magnétiques, porte-pièces utilisant le vide
61.
LIQUID FEEDING AND DISCHARGING DEVICE AND METHOD, IMMERSION OBJECTIVE SYSTEM, GENE SEQUENCER, AND BIOCHEMICAL DETECTION METHOD
A liquid feeding and discharging device is configured for applying a working liquid from a liquid feeding station to a working surface of a platform assembly. The liquid feeding and discharging device can move relative to the working surface and comprises: a liquid feeding guide pipe assembly for applying the working liquid to the working surface; a liquid discharging guide pipe assembly for extracting the working liquid from the working surface; a guide pipe structure assembly connected between the liquid feeding guide pipe assembly and the liquid discharging guide pipe assembly; a fluid interface assembly connected between the liquid feeding station and the guide pipe structure assembly; and a power assembly connected to the fluid interface assembly. The power assembly is configured for applying, by means of the fluid interface assembly, a forward driving force to the liquid feeding guide pipe assembly so as to apply the working liquid to the working surface, or a reverse driving force to the liquid discharging guide pipe assembly so as to extract the working liquid from the working surface.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
A fluid transportation system, a gene sequencer, and a fluid transportation method. The fluid transportation system comprises: a fluid temporary-storage pool (107); a positive pressure source (106) operably connected to the fluid temporary-storage pool (107) and configured to provide a positive pressure to the fluid temporary-storage pool (107); a negative pressure source (104) operably connected to the fluid temporary-storage pool (107) and configured to provide a negative pressure to the fluid temporary-storage pool (107); and a liquid storage and control unit (113) operably connected to the fluid temporary-storage pool (107) and configured to store at least one liquid and control the transportation of the at least one liquid according to the pressure state of the fluid temporary-storage pool (107).
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
63.
REACTION SYSTEM FOR PREPARING DNA NANOBALL AND USE THEREOF
Provided is a reaction system for preparing a DNA nanoball (DNB), comprising: adapters; an enzyme, wherein the enzyme comprises a DNA ligase; and a buffer solution. The adapters comprise an adapter A and an adapter B. The adapter A comprises a first strand and a second strand. The first strand comprises a sequence a, and the second strand comprises a sequence a' and a sequence b. The sequence a and the sequence a' are complementary and paired. The adapter B comprises a third strand and a fourth strand. The third strand comprises a sequence c and a sequence e, and the fourth strand comprises a sequence e', a sequence f, and a sequence b'. The sequence b' and the sequence c are complementary and paired. The sequence e and the sequence e' are complementary and paired. The sequence b and the sequence b' are complementary and paired.
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C40B 40/06 - Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés
64.
APPARATUS AND METHOD FOR COLLECTING BLOOD OR OTHER LIQUIDS OF SUBJECT
Provided is an apparatus for collecting blood or other liquids, which comprises a housing (100), a starting apparatus (200), a transmission apparatus (300), and a collecting apparatus (400). The collecting apparatus (400) is arranged in the housing (100); the transmission apparatus (300) is arranged in the housing (100) and comprises a locking assembly (310) and a driving assembly (320); the driving assembly (320) is connected to the collecting apparatus (400), at least a part of the starting apparatus (200) is connected to the locking assembly (310) in a pluggable manner, and the locking assembly (310) is configured to release the movement limitation on the driving assembly (320) to allow the driving assembly (320) to drive the collecting apparatus (400) to extend out of the housing (100) after the starting apparatus (200) is inserted, and to limit the movement of the driving assembly (320) after the starting apparatus is pulled out. The apparatus effectively solves the problem that in the prior art, a blood collecting component is prone to stretching out and hurting the human body due to pressing or touching by mistake on the blood collecting apparatus, and the internal structure arrangement of the apparatus is more reasonable.
Systems and methods for immersion imaging of a flowcell. In one example, an imaging system includes liquid ports in leading and trailing positions relative to a scanned imaging objective. The leading liquid port dispenses an immersion liquid into a space between the distal end of the imaging objective and the flowcell's cover, with the trailing liquid port collecting the immersion liquid. In another example, an imaging system includes a flowcell with a cover that is moveable relative a substrate. The system is configured to move the imaging objective and flowcell cover as a unit relative to the flowcell substrate.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 7/02 - Analyse des matériaux en mesurant la pression ou le volume d'un gaz ou d'une vapeur par absorption, adsorption ou combustion des constituants et mesure de la variation de pression ou de volume du reste
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
66.
NUCLEIC ACID LOADING REAGENT AND METHOD FOR DETECTING ADSORPTION CAPACITY OF SEQUENCING CHIP USING SAME
23222222; and (iii) the chip and the nucleic acid loading reagent are co-incubated for 10 min or above. The present invention provides a quality control scheme for the sequencing chip, which is not limited to platforms, simple to operate, low in cost, and high in speed. The present invention provides a scheme capable of quickly optimizing the loading efficiency and loading bias of a nucleic acid library, and provides a reaction reagent capable of effectively improving the loading efficiency and loading bias of a nucleic acid library.
An optical system and a detection method. The optical system comprises a first lens (10) and a second lens (20) that are sequentially arranged from an object side to an image side in an optical axis direction. The first lens (10) is arranged corresponding to the second lens (20); the second lens (20) is configured in a mobile terminal (200); the first lens (10) and the second lens (20) have the same F-number and the same focal length; and the exit pupil of the first lens (10) is located at the entrance pupil of the second lens (20). In this way, the mobile terminal (200) can be used to sequentially collect, via the first lens (10) and the second lens (20), image data of an external sample to be detected and obtain a corresponding optical image, so as to obtain a target base sequence by means of analysis, thus making a gene sequencer more miniaturized, and making gene sequencing more miniaturized, convenient and popular.
Systems and methods for immersion imaging of a flowcell. In one example, an imaging system includes liquid ports in leading and trailing positions relative to a scanned imaging objective. The leading liquid port dispenses an immersion liquid into a space between the distal end of the imaging objective and the flowcell's cover, with the trailing liquid port collecting the immersion liquid. In another example, an imaging system includes a flowcell with a cover that is moveable relative a substrate. The system is configured to move the imaging objective and flowcell cover as a unit relative to the flowcell substrate.
0obj0objobj≤0.16. By means of the cooperation of a plurality of groups of lenses, an immersion objective has a relatively small size and a relatively low mass within a relatively compact size range, and a large field of view, a high resolution and a low distortion are achieved, thereby improving the optical performance of the immersion objective.
A production scheduling method and apparatus based on medical testing, an electronic device and a medium, relating to the technical field of biomedical testing. The method comprises: determining a production process set required by sample plates by using a specified task model, wherein the specified task model comprises at least one production line, and each production line supports N production devices; searching for all production devices required by the production line corresponding to each production process in the production process set to obtain a production device set; and generating a scheduling plan on the basis of a scheduling requirement and a remaining available time period of each production device in the production device set, wherein the scheduling requirement at least comprises the sorting priorities of the sample plates, and the scheduling plan at least comprises the starting time, predicted completion time and utilization rate of each production device. The method solves the technical problem in the related art that scheduling methods for medical testing are poor in flexibility and cannot meet testing requirements.
G06Q 10/06 - Ressources, gestion de tâches, des ressources humaines ou de projetsPlanification d’entreprise ou d’organisationModélisation d’entreprise ou d’organisation
71.
CONSTRUCTION METHOD FOR REFERENCE SEQUENCE, METAGENOME DATA COMPRESSION METHOD, AND ELECTRONIC DEVICE
Provided are a construction method for a reference sequence for metagenome data compression. The method comprises: constructing a basic reference sequence database according to a sample source of metagenome data; constructing an index of the basic reference sequence database on the basis of the basic reference sequence database; and comparing a first read sequence with the basic reference sequence database according to the index of the basic reference sequence database to obtain a comparison result, wherein the first read sequence is a read sequence of some samples randomly selected in metagenome data to be compressed; and determining the sequence abundance distribution of the first read sequence according to the comparison result, and constructing a reference sequence for metagenome data compression.
The present application provides a method for analyzing droplets on the basis of volume distribution including obtaining a total volume V of a sample containing target molecules based on a system prepared using the sample. The system is emulsified into droplets. A droplet system is obtained when the droplets obtaining the sample executes an amplification reaction. A droplet image of the droplet system is obtained. A total number n of droplets included in the droplet system is obtained based on the droplet image. A droplet volume distribution of the droplet system is obtained based on the droplet image. A number j of negative droplets among the n droplets is counted. A quantitative analysis is performed for the target molecules according to the total volume V of the sample, the total number n of droplets, the droplet volume distribution information, and the number j of negative droplets.
The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
A61K 47/00 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif
C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
C07K 16/44 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel non prévu ailleurs
C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
Provided are a fluid system, a fluid transportation method, a gene sequencer, and a biochemical detection method. The fluid transportation method comprises the following steps: a) using a pump valve assembly to draw a first fluid from a fluid box, inputting the first fluid from an outlet (141) of a chip flow cell (115) into a channel (107) of the chip flow cell (115), and returning the first fluid output from an inlet (142) of the chip flow cell (115) to the fluid box; and/or b) using a pump valve assembly to draw a second fluid from the fluid box, inputting the second fluid from the inlet (142) of the chip flow cell (115) into the channel (107) of the chip flow cell (115), and returning the second fluid output from the outlet (141) of the chip flow cell (115) to the fluid box. A sample or reagent is made to be reversely conveyed from the outlet (141) to the inlet (142) of the chip flow cell (115), and the reversely loaded sample or reagent can be directly returned to a sample box (113) or a kit finally, such that the sequencing time can be shortened, the sample/reagent loading flexibility is improved, and the sequencing efficiency is increased.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
Provided is a hydrogel-based sequencing method suitable for a flowcell sequencing system and also supporting open-source sequencing systems, and which can effectively reduce costs while ensuring sequencing efficiency and accuracy.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
76.
CHIP PROCESSING DEVICE, GENE SEQUENCER, AND METHOD FOR PERFORMING BIOCHEMICAL DETECTION
Provided are a chip processing device integrated with a kit, a gene sequencer, a gene sequencer, and a method for performing biochemical detection. The chip processing device comprises a substrate extending in a first direction, as well as a kit platform and a chip platform which are assembled side by side and adjacent to each other on the substrate in a second direction transverse to the first direction. The chip platform is provided with a chip receiving area for accommodating a chip. An accommodating bin is formed on the kit platform, and the kit is received in the accommodating bin. The kit is provided with a first fluid conveying structure, and a second fluid conveying structure which is located on the chip platform and is at least partially overlapped and in communication with the first fluid conveying structure is arranged in the chip platform. The first fluid conveying structure is in fluid communication with the chip by means of the second fluid conveying structure.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
77.
GENE SEQUENCING CHIP, SLIDE AND PROCESSING METHOD THEREFOR, AND GENE SEQUENCING METHOD
The present invention provides a slide of a gene sequencing chip, a gene sequencing chip, a processing method for the slide of the gene sequencing chip, and a gene sequencing method. The slide of the gene sequencing chip comprises: a substrate comprising a gene attachment area and a gene barrier area, a plurality of gene attachment sites being arranged in the gene attachment area; a barrier coating coated on the contour edge of the gene barrier area or filled in the gene barrier area; and a liquid inlet hole and a liquid outlet hole, both the liquid inlet hole and the liquid outlet hole being formed on the substrate and communicated with the gene attachment area. According to the technical solutions of the present application, by means of changing the space containing a reagent, the reagent dose is reduced. The size of the substrate does not need to be changed, and therefore the related structure of the sequencer does not need to be changed, thus achieving a wide compatibility range at the minimum cost.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
41 - Éducation, divertissements, activités sportives et culturelles
Produits et services
Advertising; Demonstration of goods; Advertising and publicity; Provision of advertising space by electronic means and global information networks; Providing a searchable online advertising guide featuring the goods and services of online vendors; Organisation of exhibitions for commercial or advertising purposes; Providing commercial information and advice for consumers in the choice of products and services; Provision of information and advice to consumers regarding the selection of products and items to be purchased; Provision of an online marketplace for buyers and sellers of goods and services; Organization of fairs and exhibitions for commercial and advertising purposes. Arranging and conducting of colloquiums; Training; Arranging and conducting of conferences; Arranging and conducting of seminars; Arranging and conducting of symposiums; Arranging and conducting of conferences relating to commerce; Medical training and teaching; Education; Training for handling scientific instruments and apparatus for research in laboratories; Organising expert discussions in the field of genetics; Hosting of genetics seminars.
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Medical research laboratories; genetic testing for scientific research purposes; structural and functional analysis of genomes; genetic testing for scientific research purposes, namely, genetic screening for scientific research purposes; blood analysis services for scientific research purposes; DNA screening for scientific research purposes.
80.
USE OF GLYCYRRHIZIC ACID OR DERIVATIVE THEREOF IN NUCLEIC ACID DETECTION
Use of glycyrrhizic acid or a derivative thereof in nucleic acid detection. In particular, the present invention relates to a reagent comprising glycyrrhizic acid or a derivative thereof, use of glycyrrhizic acid or a derivative thereof as a nucleic acid protecting agent in nucleic acid detection, and a nucleic acid detection method.
An antioxidant composition and the use thereof in nucleic acid detection. The present invention relates to an antioxidant composition, a reagent containing the composition, a kit containing the composition or reagent, the use of the composition or reagent as a nucleic acid protection agent in nucleic acid detection, and a method for detecting a nucleic acid. The antioxidant composition contains: a saponin compound, which is selected from one or more of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rd, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rc, ginsenoside Rf and ginsenoside Re; glycyrrhizic acid, or a salt of glycyrrhizic acid, or a hydrate of a salt of glycyrrhizic acid; and adenosine 5'-monophosphate or a salt thereof, or carnosine.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
A field-of-view stitching system including: a light source module for emitting a first beam; a light guiding element for splitting the first beam into a plurality of second beams and includes an incident surface, a first exit surface, and a plurality of second exit surfaces, the second beams propagate along an optical path of the first beam; and a plurality of light modulators, a number of the plurality of light modulators is the same as a total number of the first exit surface and the plurality of second exit surfaces, each of the plurality of light modulators is configured to receive and regularly reflect one of the plurality of second beams; the light guiding element is further configured to receive and combine the second beams reflected by the light modulators to form an illuminating light. A field-of-view stitching method, a biological sample identification device and method are also provided.
A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Pipettes; Laboratory robots; Laboratory pipettes; Tubes [pipettes] for scientific use; Measuring devices; Measuring instruments; Laboratory apparatus and instruments. Pipetting apparatus for medical use; Specimen samplers for medical use; Apparatus for taking body fluid samples; Apparatus for taking blood samples; Medical instruments; Medical devices.
85.
METHOD FOR RAPIDLY CONSTRUCTING CYCLIZED LIBRARY AND CYCLIZATION ADAPTER
The present invention falls within the field of biotechnology, and specifically discloses a method for constructing a cyclized library and a ring-forming linker. The method comprises: 1) breaking a DNA sequence into fragments; 2) enabling two ends of the broken fragment to form 3′ end protrusions; and 3) cyclizing the fragment with 3′ end protrusions to form a ring-shaped library by means of a ring-forming linker, wherein the ring-forming linker is a double chain which is not completely paired and has 3′ end protrusion at both ends, and the 3′ end protrusion of the ring-forming linker is complementary to the 3′ end protrusion of the broken fragment. According to the present invention, an A-sticky end of the end is formed by means of repairing the end of and the adding A to the broken DNA fragment, and same is then complementary to the specifically designed linker T sticky end to form a cyclized structure, and the connection at the gap is completed under the action of a ligase.
A reagent storage device (1) and a reagent operating system. The reagent storage device (1) comprises a reagent compartment (10); a kit storage unit (20) arranged in the reagent compartment (10) and comprising a kit accommodating space (20A) configured to place a kit (30) having a reagent storage space (30A); and one or more puncture units (40), the puncture unit (40) comprising a sampling needle (41) having an internal channel (41A) and a puncture end (41C), wherein the puncture end (41C) is provided with a sampling needle hole (41B) communicated with the internal channel (41A); the puncture unit (40) is movably arranged relative to the kit storage unit (20) and has a first puncture working state and a second puncture working state; and the puncture unit (40) is configured such that: when the kit (30) is placed in the kit accommodating space (20A), in the first puncture working state, the sampling needle hole (41B) is in communication with the reagent storage space (30A) of the kit (30), and in the second puncture working state, the sampling needle hole (41B) is not in communication with the reagent storage space (30A) of the kit (30), and the puncture end (41C) of the sampling needle (41) of the at least one puncture unit (40) is located outside or below the bottom of the corresponding kit accommodating space (20A).
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
87.
SUPER-RESOLUTION ANALYSIS SYSTEM AND METHOD, AND CORRESPONDING IMAGING DEVICE AND MODEL TRAINING METHOD
The present application relates to the technical fields of image processing and artificial intelligence, and discloses a deep learning-based super-resolution analysis system and method. The super-resolution analysis system comprises an analysis unit; the analysis unit comprises a super-resolution implementation model; the analysis unit can be executed by a processor, and is used for constructing a super-resolution image on the basis of an input wide-field image; the super-resolution implementation model is a trained deep learning model; and a training data set for training the super-resolution implementation model and the input wide-field image come from a same imaging module. The present application further discloses a corresponding imaging device and model training method. According to the present application, simply by reconstructing a wide-field image, a super-resolution image based on a mapping relationship can be output via a deep learning algorithm, such that the number of image acquisitions is reduced, and the resolution is improved without additional time.
A reagent storage device (1) and a reagent operation system. The reagent storage device comprises: a reagent compartment (10), comprising a compartment body (11) and a compartment door (12) for closing the compartment body (11); a kit storage unit (20), arranged inside the compartment body (11) and comprising a kit accommodating space (20A), the kit accommodating space (20A) being configured to contain a kit (30) having a reagent storage space (30A); and a puncture unit (40), comprising a sample adding needle (41) arranged inside the compartment body (11). The sample adding needle (41) has an internal channel (41A) and a puncture end (41C). The puncture end (41C) is provided with a sample adding needle hole (41B) connected to the internal channel (41A). The puncture unit (40) is movably arranged relative to the kit storage unit (20) and has a first puncture working state and a second puncture working state. The puncture unit (40) is configured as follows: in a state where the kit (30) is placed in the kit accommodating space (20A), in the first puncture working state, the sample adding needle hole (41B) is connected to the reagent storage space (30A) of the kit (30), and in the second puncture working state, the sample adding needle hole (41B) is disconnected from the reagent storage space (30A) of the kit (30).
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
B65D 81/38 - Réceptacles, éléments d'emballage ou paquets pour contenus présentant des problèmes particuliers de stockage ou de transport ou adaptés pour servir à d'autres fins que l'emballage après avoir été vidés de leur contenu avec isolation thermique
B65D 81/18 - Réceptacles, éléments d'emballage ou paquets pour contenus présentant des problèmes particuliers de stockage ou de transport ou adaptés pour servir à d'autres fins que l'emballage après avoir été vidés de leur contenu fournissant une ambiance spécifique pour le contenu, p. ex. température supérieure ou inférieure à la température ambiante
89.
Method for manufacturing an apparatus for manipulating a droplet
An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.
B23P 19/04 - Machines effectuant simplement l'assemblage ou la séparation de pièces ou d'objets métalliques entre eux ou des pièces métalliques avec des pièces non métalliques, que cela entraîne ou non une certaine déformationOutils ou dispositifs à cet effet dans la mesure où ils ne sont pas prévus dans d'autres classes pour assembler ou séparer des pièces
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
Provided are a method for preparing a sequencing library and a kit for preparing a sequencing library. The preparation method comprises the following steps: (i) obtaining a nucleic acid fragment to be tested; (ii) adding a linker element pair and a cyclization element, so as to perform a ligation and cyclization reaction with the nucleic acid fragment to be tested; and (iii) performing an extension reaction to obtain the sequencing library. Further provided is a method for the single-tube preparation of a library of a nucleic acid to be tested. The method does not require the original cyclization step, and the cyclization of the library and the preparation of DNB are completed during the linker ligation process. Moreover, the traditional three-step reaction is reduced to a one-step independent reaction, which reduces time, simplifies steps, and avoids contamination.
C40B 40/06 - Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
A device for sorting cells includes a rotatable substrate (601) configured to rotate around a rotation axis (611) to generate a centrifugal force and a cover layer (441) attached to the rotatable substrate (601). The rotatable substrate (601) includes a sample reservoir (602) disposed at the center of the rotatable substrate (601), a flow path (604) coupled to the sample reservoir (602) and extending radially towards a periphery of the rotatable substrate (601) and including a bifurcation (606) into a main channel (607) and a side channel (608). The rotatable substrate (601) moves a plurality of cells stored in the sample reservoir (602) to a periphery of the rotatable substrate (601) by a centrifugal force. The cover layer (441) includes a first opening in fluid communication with the main channel (607) and a second opening in fluid communication with the side channel (608).
Disclosed herein are DNA sequencing methods involving applying a long DNA molecule to an indexed array comprising an array of transfer sites (TS). Each TS comprises a substrate and a source of clonal barcodes (SCB) attached to or situated on the substrate. Each SCB comprises many copies of a unique transferable barcode sequence, and the unique transferable barcode sequence associated with each TS is known. The unique transferable barcode sequence is transferred from the SCB portion of the TS to a location on the long DNA molecule. DNA fragments comprising the barcode sequences are recovered from the array and sequenced. Sequence reads from these fragments are assembled based on the relative positions of the TS on the array.
The methods and compositions disclosed herein relate to preparing libraries to sequence long molecules in their entirety using massively parallel short read sequencing. The methods disclosed herein generate a nested set of nucleic acid constructs for each genomic fragment and generate a plurality of nested sets for a plurality of genomic fragments. The nucleic acid constructs may be single-stranded or double-stranded. Each nucleic acid construct in each nested set comprises a barcode and target sequence portion, and nucleic acid constructs within each nested set have different lengths. The nucleic acid constructs in each nested set share a unique barcode sequence.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
94.
LIQUID TRANSFER DEVICE AND METHOD, BIOCHEMICAL SUBSTANCE REACTION DEVICE, AND BIOCHEMICAL SUBSTANCE ANALYSIS DEVICE AND METHOD
A liquid transfer device for a liquid transfer with a sample carrier of a reaction platform is provided and includes a substrate, a driving device, and a control device. The control device is used to control the driving device to drive the substrate to move towards the reaction platform. Such that the moving substrate passes through the sample carrier and transfers a liquid with the sample carrier. The liquid transfer refers to a transfer of a liquid carried by the substrate to the sample carrier and/or a transfer of the liquid on the sample carrier to the substrate. A liquid transfer method, a biochemical substance reaction device, a biochemical substance analysis device, and a biochemical substance analysis method are disclosed. A throughput of a biochemical reaction and analysis is improved, and lower the cost.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Computer software for database management; data processing
apparatus; computer software platforms, recorded or
downloadable; software as a medical device [SaMD],
downloadable; central processing units for processing
information, data, sound or images; computer software,
recorded; computer memory devices; operating system
programs; nucleic acid sequencers used for analyzing nucleic
acids in scientific research; laboratory apparatus and
instruments, namely, gene analyzers for genome information;
laboratory devices for detecting genetic sequences. Apparatus for DNA and RNA testing for medical purposes;
apparatus for the regeneration of stem cells for medical
purposes; genetic testing apparatus for medical purposes;
medical apparatus and instruments; blood testing apparatus;
diagnostic apparatus for medical purposes; apparatus used in
implementing diagnosis tests designed to detect the abnormal
prion protein; apparatus for use in medical analysis;
radiological apparatus for medical purposes.
96.
SEQUENCING KIT CONTAINING SULFYDRYL BLOCKING REAGENT AND APPLICATION OF SEQUENCING KIT
Provided are a sequencing kit containing a sulfydryl blocking reagent and an application of the sequencing kit. Also disclosed is a method for reducing a sequencing background signal. The method comprises the following steps: (1) opening a three-dimensional space structure of the enzyme protein, and exposing an active group; (2) blocking the exposed active group, wherein the blocking is irreversible blocking; (3) releasing a fluorescent substance; and (4) cleaning. For the first time, on the basis of a strategy of opening a disulfide bond of the enzyme protein and then performing irreversible blocking so as to better release the wound dyes substance, a background signal in the sequencing is reduced, and a signal-to-noise ratio and sequencing quality are improved, thereby facilitating efficient high-throughput sequencing.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
97.
SEQUENCING BUFFER AND METHOD FOR IMPROVING STABILITY OF DNTP MODIFIED WITH REVERSIBLE BLOCKING GROUP
A sequencing buffer and a method for improving the stability of dNTP modified with a reversible blocking group. The sequencing buffer comprises: a competing reagent, which can compete with dNTP modified with a reversible blocking group in reacting with a specific substance. The competing reagent in the sequencing buffer can compete with dNTP modified with a reversible blocking group in interacting with a specific substance, which reduces a change in the reversible blocking group (for example, being reduced and hydrolyzed, etc.), thereby ensuring the stability of the dNTP, allowing same to be better used in sequencing, helping to improve the quality of sequencing, greatly reducing the occurrence of premature reactions, and reducing the error rate.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
Provided in the present invention is a preservation reagent for dNTP. The preservation reagent for dNTP comprises one or more of dimethyl sulfoxide, sulfolane, tetrahydrofuran, dimethylformamide, dimethylacetamide, N,N-dimethylformamide, N-methylformamide and N-methylpyrrolidone. The preservation reagent for dNTP of the present invention can be used for dissolving dNTP, particularly dNTP modified with a reversible blocking group, and improving the stability of dNTP, so that dNTP can be preserved for a long time, which is beneficial for ensuring the accuracy and efficiency of amplification and sequencing.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
99.
SEQUENCING KIT CONTAINING THIOL BLOCKING REAGENT, AND USE OF SEQUENCING KIT
A sequencing kit containing a thiol blocking reagent, and a use of the sequencing kit. Further disclosed is a method for reducing a sequencing background signal, comprising the following steps: (1) opening a three-dimensional spatial structure of an enzyme protein, and exposing an active group; (2) blocking the exposed active group, wherein the blocking is irreversible blocking; (3) releasing a fluorescent substance; and (4) cleaning. On the basis of the strategy that a disulfide bond of the enzyme protein is first opened and then is irreversibly blocked so as to better release a wound dye substance, a background signal in sequencing is reduced, the signal-to-noise ratio and the sequencing quality are improved, and efficient high-throughput sequencing is facilitated.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
100.
EXCISION REAGENT CONTAINING GLYCINE-NAOH BUFFER AND USE THEREOF IN NUCLEIC ACID SEQUENCING
Provided are an excision reagent containing a glycine-NaOH buffer and the use thereof in nucleic acid sequencing. Further provided are a sequencing kit containing the excision reagent, a method for excising a blocking group at the 3' end of a polynucleotide, and a sequencing method. During sequencing of the polynucleotide according to the method, the efficiency of excising the blocking group at the 3' end can be improved, the sequencing Q30 is effectively increased, Lag and Runon are reduced, and the sequencing quality is improved.
