Provided are a gene expression matrix optimization method, an electronic device, and a storage medium. The method comprises: acquiring cell data of a plurality of cells, wherein the cell data comprises an initial gene expression matrix of each of the plurality of cells; determining a preset number of nearest neighbor cells of a target cell among the plurality of cells on the basis of the cell data; determining a gene expression distance between the target cell and each nearest neighbor cell, determining a distance parameter on the basis of a target non-zero value among the gene expression distances, and determining, on the basis of the distance parameter, a weight parameter corresponding to each nearest neighbor cell; and on the basis of the weight parameter corresponding to each nearest neighbor cell and the initial gene expression matrix of each nearest neighbor cell, obtaining a target gene expression matrix obtained by smoothing of the target cell. By using the described method, the noise in a gene expression matrix can be reduced to improve the accuracy of performing downstream analysis on the basis of the gene expression matrix.
A method for embedding a nanopore protein into a biomimetic membrane and the use thereof. The method comprises: preparing a biomimetic membrane of a multi-block copolymer, wherein the multi-block copolymer comprises a hydrophilic segment and a hydrophobic segment; and embedding a nanopore protein into the biomimetic membrane, wherein the multi-block copolymer comprises a copolymer of two or more blocks, the biomimetic membrane comprises an aromatic ring structure, the aromatic ring structure is linked between the hydrophilic segment and the hydrophobic segment of the multi-block copolymer, and/or the nanopore protein comprises an aromatic ring structure, and the aromatic ring structure is located on an amino acid in the transmembrane region of the nanopore protein facing the biomimetic membrane. By means of the method, the number of single pores and/or the single-pore retention rate of a biomimetic membrane during embedding can be improved.
B01D 71/82 - Matériaux macromoléculaires non prévus spécifiquement dans un seul des groupes caractérisés par la présence de groupes déterminés, p. ex. introduits par un post-traitement chimique
3.
BIOCHIP, PREPARATION METHOD THEREFOR AND USE THEREOF
A biochip, a preparation method therefor and the use thereof. The biochip is a double-sided porous nano-biochip, which has an anodized nanostructure on the surface thereof; and the surface of the chip can be chemically modified to provide, for RNA synthesis, linking molecules required for a synthesis reaction, thus enabling the attachment of monomers to the surface of the biochip. The biochip can be prepared by using an anodization method which is easily operated and is low cost, thereby lowering the difficulty of the preparation process, and providing synthetic products having a higher loading capacity at a lower price.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
4.
METHOD AND APPARATUS FOR TISSUE TRACKING CELL FREE RNA
Provided are a method and apparatus for performing tissue tracking of cell free RNA, a device, a readable storage medium, a computer program product, and a computer program. The method for performing tissue tracking of cell free RNA comprises: using one or more transcriptome data sets to construct a tissue characteristic gene matrix; on the basis of a cell free RNA expression profile of a sample and the tissue characteristic gene matrix, calculating a tissue contribution score; and, on the basis of the tissue contribution score, performing tissue tracking of the cell free RNA.
A structured-light illumination system, a structured-light illumination method, and a super-resolution optical system. The structured-light illumination system comprises: at least two light sources (1), wherein the light sources (1) are used to generate light beams of a preset wavelength; at least two gratings (2) respectively located on the optical paths of the at least two light sources (1), wherein the gratings (2) are used to generate interference fringes; displacement platforms (3), wherein the gratings (2) are disposed on the displacement platforms (3), and the displacement platforms (3) are used to move the gratings (2) so as to perform phase switching; and a light beam converging and guiding unit (4), wherein the optical paths intersect at the light beam converging and guiding unit (4), and the light beam converging and guiding unit (4) is used to guide the light beams to converge to an object space. The structured-light illumination system can perform rapid angle and phase switching on structured-light fringes and increases imaging speed, thus increasing the throughput of the optical system, and greatly reducing imaging costs while improving the resolution of the optical system.
An automatic liquid replenishing device and method for a sequencer. In the automatic liquid replenishing device, a first connector is arranged above a sequencer and is communicated with a liquid inlet of a sequencing chip; a second connector is communicated with a first liquid storage device; a third connector is communicated with a second liquid storage device; a fourth connector is communicated with a third liquid storage device; two ends of a first connecting pipe are respectively communicated with the first connector and the second connector; two ends of a second connecting pipe are respectively communicated with the first connector and the third connector; two ends of a third connecting pipe are respectively communicated with the third connector and the fourth connector; a fourth connecting pipe is communicated with the fourth connector; a first peristaltic pump is arranged on the path of the first connecting pipe; and a second peristaltic pump is arranged on the path of the fourth connecting pipe.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 1/14 - Dispositifs d'aspiration, p. ex. pompesDispositifs d'éjection
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
7.
EMBRYO-LIKE MODEL, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCES (Chine)
Inventeur(s)
Esteban, Miguel
Lai, Yiwei
Mazid, Md·abdul
Li, Jinxiu
Jia, Wenqi
Fu, Lixin
Zuo, Jing
Li, Wenjuan
Wu, Liang
Abrégé
Provided is an embryo-like model construction method. The method comprises: culturing primordial stem cells in vitro or injecting primordial stem cells into an immunodeficient animal for differentiation, so as to obtain an embryo-like model. By means of the method, during the preparation of the embryo-like model, there is no need to add cytokines and small molecule pathway interference factors, and there is no need to perform gene editing and only a single initiating cell type is used. The method has the advantages of simplicity and high repeatability, and is beneficial to large-scale preparation and application. Moreover, the prepared embryo-like model has all extraembryonic cell lineages and all embryonic cell lineages other than trophectoderm, can simulate the development process of a post-implantation embryo of Carnegie stage 3 to Carnegie stage 9 neurula, and then can be used for preparation of a variety of progenitor cell lineages, disease modeling, drug screening, and scientific researches.
Provided in the present invention are a porin-membrane fusion method, a porin insertion buffer solution and the use. Said fusion method comprises: providing a first solution at a first side of a membrane and providing a second solution at a second side of the membrane; applying a voltage to both sides of the membrane to perform porin-membrane fusion; measuring a current in the solution; and when the current increases which indicates a porin has been inserted into the membrane, then adjusting the voltage to 0 V, thereby completing the porin-membrane fusion. The first solution comprises a first buffer solution containing the porin, and the second solution comprises a second buffer solution which does not contain the porin, the osmotic pressure of the first solution being greater than the osmotic pressure of the second solution. The present invention can resolve the issue of the single-pore ratio during porin-membrane fusion in the prior art, and is suitable for the field of biological analysis and detection.
G01N 27/26 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant des variables électrochimiquesRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en utilisant l'électrolyse ou l'électrophorèse
Disclosed in the present invention is an immune repertoire sequencing method, comprising the following steps: 1) constructing a cDNA library of a sample, cDNA in the cDNA library being linked with position information of the cDNA on the sample; 2) carrying out targeted enrichment of cDNA of a TCR gene and/or a BCR gene in the cDNA library to obtain an immune repertoire of the sample; and 3) sequencing the immune repertoire. According to the method of the present invention, targeted enrichment and high-throughput sequencing of a TCR sequence and/or a BCR sequence are innovatively achieved, and at the same time, gene expression information and spatial position information of each cell in the space are detected.
A microscopic imaging apparatus, a sequencing device, and a microscopic imaging method. The microscopic imaging apparatus comprises a light source (10), an illumination light path assembly, an imaging light path assembly, and an imaging processing apparatus (14); the illumination light path assembly and the imaging light path assembly are arranged opposite to each other, and an illumination chip (12) is arranged between the illumination light path assembly and the imaging light path assembly; the light source (10) irradiates, by means of the illumination light path assembly, light having different irradiation angles to the illumination chip (12), so that the illumination chip (12) generates plasma structured light, wherein a substrate of the illumination chip (12) is made of a transparent material; and the imaging processing apparatus (14) captures, by means of the imaging light path assembly, an original image of a fluorescence signal of the illumination chip (12) under the irradiation of light having different irradiation angles.
Disclosed in the present invention are a liquid transfer device, a reaction device and an analysis device, the liquid transfer device being used for guiding a reaction liquid to a reaction surface of a sample carrier. A fixing portion comprises a fixing structure, the fixing structure being used for fixing a sample carrier. A first substrate comprises a first wall surface, the first substrate having a first position and a second position which are relative to the fixing portion. When the first substrate is located at the first position, the first wall surface is used for obtaining a reaction liquid, and when the first substrate is located at the second position, the first wall surface is used for guiding the reaction liquid to a reaction surface. A first driving portion is configured to drive the first substrate to perform position switching between the first position and the second position. The Mohs hardness of the first substrate is greater than or equal to 5H. The present invention can effectively control the distance between the first wall surface and the reaction surface of the sample carrier, thereby ensuring the quality of reaction liquid transfer, and avoiding damage to the reaction surface caused when the first wall surface is in contact with the reaction surface, or failure to completely transfer the reaction liquid to the reaction surface caused by an excessively large distance between the first wall surface and the reaction surface.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
12.
LIQUID TRANSFER DEVICE, REACTION DEVICE, ANALYSIS DEVICE, AND LIQUID TRANSFER METHOD
Disclosed in the present invention are a liquid transfer device, a reaction device, an analysis device, and a liquid transfer method. The liquid transfer device is used for transferring a liquid carried by a first substrate to a sample carrier. The liquid transfer device comprises a fixing part and a first supporting part. The fixing part comprises a fixing structure, and the fixing structure is used for fixing the sample carrier. The first supporting part and the fixing structure are spaced oppositely in a first direction. The first supporting part has a first supporting surface. The first supporting surface is configured to at least partially face a reaction surface of the sample carrier after the sample carrier is fixed by the fixing structure. The first supporting surface is used for supporting the first substrate, so that the liquid carried by the first substrate supported by the first supporting surface is transferred to the reaction surface of the sample carrier fixed by the fixing structure. According to the liquid transfer device, the reaction device, the analysis device, and the liquid transfer method of the present application, shaking of the first substrate can be effectively inhibited, thereby preventing the first substrate from scraping the sample carrier and improving the stability of liquid transfer.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
13.
ELECTROCHEMICAL BUFFER SOLUTION FOR BIOLOGICAL NANOPORES AND PREPARATION METHOD THEREFOR
The present invention relates to the field of biochemical detection, and specifically relates to an electrochemical buffer solution for biological nanopores and a preparation method therefor. Provided is an electrochemical buffer solution which has high cost performance and is capable of reducing the loss of biological nanopores. The buffer solution comprises potassium ferricyanide, potassium ferrocyanide, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, EDTA and mannitol. When the electrochemical buffer solution is used for biological nanometer detection, the loss rate of biological nanopores can be significantly reduced, the stability of biological nanopores is improved, the flux of detection is increased, and the problem of biological nanopore loss is effectively overcome. Moreover, the electrochemical buffer solution can also be used in monomolecular sensing technology and/or ultra-trace element detection technology, and can be used as a buffer reagent for improving the stability of detection.
G01N 27/26 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant des variables électrochimiquesRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en utilisant l'électrolyse ou l'électrophorèse
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
A DNA polymerase and the use thereof. The DNA polymerase is: 1) a protein having an amino acid sequence as shown in SEQ ID NO: 1 or 2; or 2) a protein having an amino acid sequence that has 80% or more, more preferably 90% or more, and further preferably 95% or more homology to the amino acid sequence as shown in SEQ ID NO: 1 or 2, and having a DNA polymerase activity. The DNA polymerase has a relatively high amplification rate, a relatively good thermal stability, and 5'-3' polymerization activity and 3'-5' exonuclease activity. During DNA amplification, the DNA polymerase can be used to perform DNA amplification at a relatively high PCR extension rate, thus being able to better meet market demand, and having a great application potential.
Disclosed in the present disclosure are a focusing method and apparatus based on linear array scanning, a storage medium, and an electronic device. The method is applied to a biological sample scanning system, and the biological sample scanning system at least comprises: a camera and an objective lens. The method comprises: acquiring spatial position information of an anchor point corresponding to a sample to be scanned, wherein the spatial position information of the anchor point represents the position of a focused target objective lens; on the basis of the spatial position information of the anchor point, determining curved surface information corresponding to the anchor point, wherein the curved surface information corresponding to the anchor point at least comprises target objective lens positions of a plurality of sampling points obtained by sampling the spatial position information of the anchor point; acquiring the current objective lens position corresponding to the current scanning point in the sample to be scanned; and on the basis of the current objective lens position and the curved surface information corresponding to the anchor point, adjusting the current objective lens position to carry out focusing operation on the objective lens. By means of the described solution, the present application improves the focusing efficiency of the objective lens.
H04N 3/14 - Détails des dispositifs de balayage des systèmes de télévisionLeur combinaison avec la production des tensions d'alimentation par des moyens non exclusivement optiques-mécaniques au moyen de dispositifs à l'état solide à balayage électronique
16.
LABELING AND ANALYSIS METHOD FOR SINGLE-CELL NUCLEIC ACID
Provided are a method for positioning and labeling a nucleic acid molecule, and a method for constructing a nucleic acid molecule library for single-cell transcriptome sequencing, which relate to the technical fields of single-cell transcriptome sequencing and biomolecular space information detection. Further provided are a nucleic acid molecule library constructed by using the method, and a kit for implementing the method.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
17.
NUCLEIC ACID POLYPEPTIDE COMPLEX AND USE THEREOF IN PEPTIDE SEQUENCING
Provided are a nucleic acid polypeptide complex and the use thereof in peptide sequencing. The nucleic acid polypeptide complex comprises a first nucleic acid fragment, a polypeptide to be tested and a composite nucleic acid fragment which are connected in sequence, wherein the composite nucleic acid fragment comprises a nucleic acid sequence capable of forming a hairpin structure; after the hairpin structure is formed, the composite nucleic acid fragment comprises a double-stranded nucleic acid and a single-stranded nucleic acid, one end of the single-stranded nucleic acid is connected to the double-stranded nucleic acid, and the other end of the single-stranded nucleic acid is connected to the polypeptide to be tested. The detection of any polypeptide sequence can be achieved by means of guiding the polypeptide with different charges by the nucleic acid sequence to pass through the nanopore.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
18.
COMBINED ELEMENT FOR SCREENING APTAMER AND USE THEREOF, AND HIGH-THROUGHPUT SCREENING METHOD FOR APTAMER
A high-throughput screening method for an aptamer, and a use. An implementation route is divided into three parts, a first part is construction of a library of a pre-enriched aptamer and sequencing same, a second part is generation of an aptamer cluster on a slide, and a third part is combination of a target protein and the aptamer cluster. Provided is a method for high-throughput rapid detection of an aptamer. The method has the beneficial effects: sequencing time, sequence synthesis and affinity re-verification time are saved, the affinity verification costs are greatly saved, rapid screening of aptamers can be promoted, and the actual use of aptamers is promoted along with the increase in the types and numbers of aptamers. The existing platform BGISEQ 500 of Beijing Genomics Institute can be used to grow an aptamer cluster on a chip by using DNB as a template, and the use of sequencers is expanded by integrating sequencing, sequence synthesis and affinity identification.
An engineered biological containment system, comprising: a first nucleic acid, the first nucleic acid comprising at least a fragment portion of a first essential gene; a second nucleic acid, the second nucleic acid comprising at least a fragment portion of a second essential gene; a third nucleic acid, the third nucleic acid activating the transcription of the first nucleic acid; and a fourth nucleic acid, the fourth nucleic acid encoding a non-natural amino acid encoding tool. The second nucleic acid comprises one or more nucleotide sequences encoding a first termination codon and optionally a nucleotide sequence located at the end position of a second-essential-gene encoding region and encoding a second termination codon.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
C12N 15/65 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression utilisant des marqueurs
Provided in the present invention are a method for preparing a strand-specific library for the rapid detection of various types of RNAs, and a high-throughput sequencing method. A polyadenylic acid (poly A) tail is artificially added at the 3' terminal of various RNAs by using a poly A polymerase, and a single-stranded cDNA is synthesized using a poly-deoxythymidine ribonucleotide primer with deoxyuridine under the action of a reverse transcriptase. The obtained single-stranded cDNA molecule is subjected to a series of reactions, and the reaction product is finally amplified by means of PCR to obtain a strand-specific library of various types of RNAs. The library sample can be subjected to on-machine sequencing.
The present invention provides an underwater sequencer. The underwater sequencer comprises: a sample extraction device, used to extract an underwater microbial sample; a library construction device, an input port of the library construction device being connected to an output port of the sample extraction device, the library construction device being used to construct a gene library of the microbial sample; and a sequencing device, an input port of the sequencing device being connected to an output port of the library construction device, the sequencing device being used to perform gene sequencing on the gene library. Aiming at the relevant needs of underwater sequencing, the present invention integrates an extraction device, a library construction device, and a sequencing device, can automatically perform nucleic acid extraction, library construction, and sequencing without human intervention, and is easy to operate. The integrated underwater sequencer also has the advantages of small size and low power consumption.
Disclosed in the present invention is a method for repeated utilization of a microarray chip, the method comprising the following steps: 1) using a strong alkali or protease to treat a microarray chip which fails in quality control; and 2) re-preparing a new microarray chip from the microarray chip treated in step 1), so as to realize repeated utilization of the microarray chip. In the present invention, a strong alkali or protease is used to treat an SC which fails in quality control, and an FC is prepared from the SC; and protease is used to treat an SC which fails in quality control, and a new SC is re-prepared from the SC. By means of the present invention, a microarray chip which fails in quality control is re-utilized to reduce the cost of raw materials and the sequencing cost of the chip.
Provided in the present invention is a nanopore sequencing method using a closer. The method of the present invention enables a sequencing library to only bind within the nanopore capture range, ensuring that the library is efficiently captured by the nanopores during sequencing, thus improving the utilization efficiency of the library.
Disclosed in the present disclosure are a biochip scanning method and apparatus, and a storage medium and an electronic device. The method comprises: in response to a chip selection instruction, determining, from among a plurality of biochips, a chip to be scanned; parsing a chip scanning instruction to determine a target focusing mode; determining a scanning parameter corresponding to the target focusing mode; on the basis of the scanning parameter, performing a scanning operation on said chip, so as to obtain scanning data; and in response to a scanning end instruction, acquiring scanning data that is generated after the scanning operation, and generating a target scanning image on the basis of the scanning data.
Disclosed in the present invention are a sub-sequence search method for a gene sequence, a comparison method, a system, and a device. The sub-sequence search method comprises: acquiring a preset number of pieces of sequencing data of a gene sequence to be searched; sequentially acquiring, from a reference gene sequence, base data of a search interval corresponding to each piece of sequencing data, and performing a search on the basis of an acquisition result, wherein the search interval corresponding to each piece of sequencing data is determined on the basis of a base sequence to be searched of each piece of sequencing data; and repeatedly acquiring base data of a search interval of the next search, and performing a search on the basis of an acquisition result, so as to obtain a target sub-sequence, wherein the search interval of the next search is determined according to the next base to be searched of each piece of sequencing data and a search interval of the previous search. In the search method in the present invention, base data of a search interval is pre-fetched, and by means of batch processing of sequencing data, the calculation of the other sequencing data can be processed when waiting for a pre-fetching result, thereby improving the efficiency of a gene search.
Disclosed in the present invention is a chip substrate preparation method based on a glass sheet. The chip substrate preparation method comprises the following steps: making the surface of a glass sheet carry an amino, and reacting a coupling reagent having an azide group with the amino on the surface of the glass sheet, so as to form an amide bond. Further disclosed in the present invention is a chip preparation method. On the basis of the chip substrate preparation method, the chip preparation method comprises the following steps: reacting a 5'-end-modified probe with the azide group of the coupling reagent, and combining the probe on the surface of the glass sheet. In the present invention, the glass sheet is used to replace a silicon wafer, thereby reducing the cost of the chip raw material, also ensuring the number and quality of the probes of the chip, and improving the use stability of the chip.
A scanning imaging system (100) for a biochip, comprising a damping module (1), an objective table (2) and an optical engine module (3), wherein the damping module (1) comprises a damping bottom plate (11) and optical engine supporting frames (12) located on opposite sides of the damping bottom plate (11); in a first direction (z), the height of the optical engine supporting frames (12) is greater than the thickness of the damping bottom plate (11); the first direction (z) is perpendicular to a plane where the damping bottom plate (11) is located; the objective table (2) is located on the damping bottom plate (11) and is configured to carry a biochip (7); the objective table (2) can move in a second direction (x) and a third direction (y); in the second direction (x) and the third direction (y), the maximum moving distance of the objective table (2) is at least 20 cm; the second direction (x) and the third direction (y) are orthogonal to each other and are parallel to the plane where the damping bottom plate (11) is located; the optical engine module (3) is supported by two optical engine supporting frames (12); the optical engine module (3) covers an area where the damping bottom plate (11) is located, and is not in contact with the objective table (2); and the optical engine module (3) comprises an objective (32) and an optical imaging unit, and is configured to perform scanning imaging on the biochip (7).
G01Q 30/18 - Moyens pour protéger ou isoler l'intérieur d'une enceinte d'échantillonnage contre les conditions ou les facteurs environnementaux externes, p. ex. les vibrations ou les champs électromagnétiques
28.
ORGANIC SILICON COMPOSITE STRUCTURE, PREPARATION METHOD, AND MICROFLUIDIC CHANNEL STRUCTURE OF ORGANIC SILICON COMPOSITE STRUCTURE
The present invention provides an organic silicon composite structure, a preparation method, and a microfluidic channel structure of the organic silicon composite structure. The organic silicon composite structure comprises a non-silicon substrate layer, an intermediate layer, and an organic silicon layer; two surfaces of the intermediate layer are respectively bonded with the non-silicon substrate layer and the organic silicon layer; and the intermediate layer is an inorganic silicon layer. The intermediate layer is respectively bonded with the non-silicon substrate layer and the organic silicon layer, so that bonding between the non-silicon substrate layer and the organic silicon layer is achieved; moreover, due to a high-temperature organic reagent being not used to soak the non-silicon substrate layer during bonding, the material mechanical properties and the stability of the surface properties of the non-silicon substrate layer are kept, and the added intermediate layer basically does not affect the material mechanical properties of a substrate. An apparatus used during manufacturing is a common apparatus in a clean room for producing a semiconductor device, the operation is simple and direct, and the success rate is high.
B32B 5/00 - Produits stratifiés caractérisés par l'hétérogénéité ou la structure physique d'une des couches
G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
G01N 37/00 - Détails non couverts par les autres groupes de la présente sous-classe
29.
PROTEIN FUNCTIONAL ANNOTATION METHOD AND APPARATUS, ELECTRONIC DEVICE, AND STORAGE MEDIUM
The present disclosure provides a protein functional annotation method and apparatus, an electronic device, and a storage medium. The method comprises: separately acquiring a sequence feature, a pre-trained sequence feature, and a preset annotation feature on the basis of a protein sequence; performing feature extraction on the preset annotation feature to obtain a first learning feature; performing feature extraction on the sequence feature and the pre-trained sequence feature to obtain a second learning feature; fusing the first learning feature and the second learning feature to obtain a first fused feature; and processing the first fused feature to obtain protein functional annotation information. Compared with the related art, the present disclosure relates to acquiring a sequence feature, a pre-trained sequence feature, and a preset annotation feature on the basis of a protein sequence, and fusing the three different aspects of features, such that protein functional annotation information obtained is more accurate.
Disclosed in the present invention are a DNA sequence library for continuous data storage, a method for continuous data storage, and a corresponding reading method and reading system. The reading method comprises: 1) capturing a DNA sequence in a DNA sequence library using a capture localization region, wherein the capture localization region corresponds to spatial information of continuous data; and 2) performing sequencing on a data region of the DNA sequence, wherein the data region corresponds to continuous data content, and restoring the continuous data content as the sequencing proceeds. The present invention innovates a reading mode for DNA data storage to realize instantaneous decoding, and solves the problem of low information reading efficiency in DNA data storage, thereby increasing the possibility of large-scale application of DNA data storage.
Disclosed are a method and apparatus for DNA storage encoding/decoding and rules thereof. The method comprises: executing single-molecule sequencing on a reference sequence, and acquiring actual sequencing data of the single-molecule sequencing; comparing the actual sequencing data with reference data of the reference sequence, counting the frequency, in the actual sequencing data, of sequencing errors of each sequence segment having a length of k, and calculating the proportion, in the actual sequencing data, of sequencing errors of each sequence segment having a length of k, that is, the error rate; and performing removal by using a sequence segment, the error rate of which exceeds a threshold, as a limiting condition. In the method of the present invention, the steps of DNA storage encoding/decoding are simplified, the time sequence of the steps is eliminated by using a threshold, and the complexity of data processing is reduced.
Provided in the present application are a cell type annotation method and apparatus, and a device and a storage medium. The method comprises: clustering cell sequencing data to obtain a plurality of cell populations; analyzing a differential high-expression component list of each cell population, wherein the differential high-expression component list includes differential high-expression components (the components refer to genes or proteins) in the cell population which are sequenced according to specificity scores in descending order; and for each cell population, according to the sequence of preset marker components corresponding to cell types in the differential high-expression component list of the cell population, determining the probability of the cell population belonging to each cell type, and determining a corresponding cell type with the highest probability to be the cell type to which the cell population belongs, wherein the sequence of the marker components corresponding to the cell types in the differential high-expression component list of the cell population is positively correlated with the probability of the cell population belonging to the cell type. In the present solution, the effect of a marker component on a cell type to which a cell population belongs is correspondingly enhanced or reduced according to the magnitude of the specificity of the marker component in the cell population, thereby improving the accuracy of an annotation result.
The present invention provides a preparation method for an extrachromosomal circular DNA library, a sequencing method, a kit, and a use thereof. The preparation method comprises: extracting total DNA in a sample and digesting linear DNA in the total DNA to obtain a digestion product, the digestion product comprising extrachromosomal circular DNA; directly carrying out rolling circle amplification on the digestion product without purification, to obtain an amplified product; and directly carrying out library preparation on the amplified product without purification, to obtain an extrachromosomal circular DNA library.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
34.
METHOD, APPARATUS, AND SYSTEM FOR DATA ACQUISITION
Embodiments of the present disclosure provide a method, apparatus, and system for data acquisition, which relate to the technical field of data processing. The method comprises: sectioning a biological tissue sample, and performing time-and-space omics sequencing on the sections to obtain expression level data; performing image acquisition on the sectioned biological tissue sample to obtain image data; performing registration on the expression level data and/or the image data to generate an outer-layer contour and/or a partition contour of the biological tissue sample; and generating three-dimensional display data for the biological tissue sample according to the outer-layer contour and/or the partition contour. The present disclosure provides a whole set of apparatus and method for data acquisition, and a registration method for expression level data and tissue section image data of time-and-space omics sequencing. Aiming at in situ capture time-and-space omics technology, combined with optical imaging information and assistance of rigid and elastic registration of continuous tissue sections, three-dimensional reconstruction is achieved; therefore, auxiliary visual information positioning of inner and outer tissue contours is obtained, and time-and-space omics data with large size and high resolution is effectively processed and displayed.
G06T 7/33 - Détermination des paramètres de transformation pour l'alignement des images, c.-à-d. recalage des images utilisant des procédés basés sur les caractéristiques
35.
DATA PROCESSING METHOD AND APPARATUS FOR IMAGE REGISTRATION, AND ELECTRONIC DEVICE
The present application relates to a data processing method and apparatus for image registration, and an electronic device, and relates to the technical field of data processing. The method comprises: firstly acquiring a first image and a second image to be registered; then registering the first image and the second image; then inputting the registered first image and second image into a machine learning model for calculation to obtain deformation field information, wherein the machine learning model is obtained by performing model training in advance according to image registration data of a sample image, and the image registration data comprises: the sample image and a deformed and corrected sample image; and determining corresponding coordinate information after image registration according to the deformation field information. The technical solutions of the present application solve the problem of elastic registration of multi-modal images, are easy to implement, and can improve the accuracy of image registration.
The present disclosure provides a spatiotemporal transcriptomics slice alignment method and apparatus. The main technical solution comprises: obtaining two adjacent slices, wherein each slice comprises at least one type of expression level matrixes, and each expression level matrix comprises spots of a plurality of pieces of spatiotemporal transcriptomics expression level data; respectively determining weights corresponding to the same type of spots in each slice, wherein the same weight is assigned to the same type of spots, and weights assigned to different types of spots are different (102); and according to the weight corresponding to each type of spots and a preset regularization coefficient, respectively calculating probability transition matrixes to obtain an alignment score of each spot in the expression level matrix corresponding to each category on the two slices (103). Compared with the related art, the same weight is assigned to the same type of spots in each slice, and the weights assigned to different types of spots are different, so that the accuracy of the probability transition matrixes obtained on the basis of weight calculation is improved, and the accuracy of alignment realized on the basis of the probability transition matrixes is thus improved.
G16B 20/30 - Détection de sites de liaison ou de motifs
G06T 7/33 - Détermination des paramètres de transformation pour l'alignement des images, c.-à-d. recalage des images utilisant des procédés basés sur les caractéristiques
37.
CELL CONTOUR RENDERING METHOD, APPARATUS AND SYSTEM
A cell contour rendering method, comprising: acquiring slice cell data, and performing segmentation processing on the slice cell data, so as to obtain segmented data; on the basis of the slice cell data, performing subdivision processing and/or area-reduction processing on contour points of cells in a slice, so as to generate multi-layer fineness vertex index data; writing the multi-layer fineness vertex index data into corresponding segmented data, so as to generate multi-layer fineness data to be subjected to rendering; and performing cell contour rendering according to the data to be subjected to rendering.
Disclosed are a registration method and apparatus for spatio-temporal transcriptome slices, an electronic device, and a storage medium. The method comprises: acquiring each target spot of a target slice, and respectively acquiring, in a probabilistic transfer matrix corresponding to an adjacent slice, an index vector of a spot having the maximum probability transfer value; performing rigid registration on the target slice on the basis of the probabilistic transfer matrix to obtain a registered target slice; calculating a deformation field of the registered target slice on the adjacent slice according to coordinates of the spot on the adjacent slice corresponding to the index vector and coordinates of the target spot; and respectively finding change values of coordinates in deformation fields of two adjacent slices, and registering the coordinates of each spot of the target slice by using the change values. After rigid registration is performed on the target slice, the deformation of the slice is registered to restore the real shapes of a plurality of slices in an organism, thereby further improving the accuracy of a registration result.
A deep reactive ion etching method of organic silicon, comprising: S110, providing an organic silicon substrate, and forming a patterned mask layer on the surface of the substrate; S120, placing into an etching system the organic silicon substrate on which the mask layer is formed, setting etching parameters, and introducing etching gas to etch the organic silicon substrate; and S130, removing the mask layer to obtain an organic silicon material subjected to ion etching. By selecting the combination of etching gases and accurately controlling the flow of the etching gases, an etching precision, an etching depth-to-width ratio, and an etching speed which are far higher than those obtained by using existing means can be obtained.
A property restoration method for a flexible microstructure having a high aspect ratio. The method comprises: placing a flexible microstructure substrate (2) in a first property restoration solvent for ultrasonic treatment, wherein the first property restoration solvent is used for immersing the flexible microstructure substrate (2); taking the flexible microstructure substrate (2) out of the first property restoration solvent, and transferring the flexible microstructure substrate (2) into a chamber containing a second property restoration solvent (3), wherein the second property restoration solvent (3) is used for immersing the flexible microstructure substrate (2); introducing a supercritical fluid to replace the second property restoration solvent (3) in the chamber; and totally replacing the second property restoration solvent (3) and then discharging the supercritical fluid. The flexible microstructure substrate (2) soaked in the first property restoration solvent is ultrasonically cleaned, so that the flexible microstructure substrate (2) that has agglomerated, deformed or collapsed can be separated. The second property restoration solvent (3) for soaking the flexible microstructure substrate (2) is then replaced with the supercritical fluid (6), and the stress accumulated on the flexible microstructure substrate (2) is released, so that the flexible microstructure substrate (2) is dried and restored in property. Thus, it is possible to prepare a flexible microstructure substrate (2) having a high aspect ratio in a mold forming mode.
A biosensor chip packaging structure (100), a PCB (201), and a micro-fluidic detection apparatus. The biosensor chip packaging structure comprises: a sensor chip (1), an integrated circuit chip (2) and a substrate (3), wherein the sensor chip (1) is used for being in contact with an external substance and converting a measured parameter into a measurement signal; the integrated circuit chip (2) is used for processing the measurement signal; and the sensor chip (1) and the integrated circuit chip (2) are independently provided on the substrate (3), and the sensor chip (1) and the integrated circuit chip (2) are electrically connected by means of the substrate (3). In the structure of biosensor chip packaging, the scrapping of a sensor chip does not affect an integrated circuit chip, thereby decreasing a scrap rate, and reducing the process difficulty; and the sensor chip can be subjected to measurement or maintenance independently, thereby improving the economical efficiency of maintenance.
B81B 7/02 - Systèmes à microstructure comportant des dispositifs électriques ou optiques distincts dont la fonction a une importance particulière, p. ex. systèmes micro-électromécaniques [SMEM, MEMS]
B81C 1/00 - Fabrication ou traitement de dispositifs ou de systèmes dans ou sur un substrat
A focusing method and system. The focusing method comprises: acquiring, captured by a photodetector (8), an electrical signal corresponding to a light spot (300) reflected by a surface of an object (7) to be measured; wherein the photodetector (8) comprises first pixels (100) and second pixels (200), an electrical signal corresponding to a first pixel (100) being a first electrical signal, and an electrical signal corresponding to a second pixel (200) being a second electrical signal; calculating the sum of a first electrical signal and a second electrical signal, to act as a sum signal; determining whether the sum signal is greater than or equal to a preset threshold; and in response to the sum signal being greater than or equal to the preset threshold, using a first preset relationship to determine a current amount of defocus, and using a second preset relationship to determine a speed of an objective lens (6) corresponding to the current amount of defocus, so as to control the objective lens (6) to move and focus at said speed.
Provided are a method for preparing a nucleic acid-polypeptide complex, a nucleic acid-polypeptide complex prepared by the method, and a use of the method. The method for preparing the nucleic acid-polypeptide complex can couple one or more polypeptides having different lengths and sequences with one or more nucleic acids, and the method has a wide application range, and has the advantages of high yield and easy purification of products.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Chemical reagents, other than for medical or veterinary
purposes; chemical preparations for scientific purposes,
other than for medical or veterinary use; biochemical
preparations for scientific purposes; biological
preparations, other than for medical or veterinary purposes;
test paper, chemical; biological preparations for use in
cell cultures, other than for medical or veterinary
purposes; chemical substances for analyses in laboratories,
other than for medical or veterinary purposes; chemical
preparations for analyses in laboratories, other than for
medical or veterinary purposes; diagnostic reagents and
preparations, except for medical or veterinary use;
biological tissue cultures, other than for medical or
veterinary purposes. Biological preparations for medical purposes; medical
diagnostic reagents for the analysis of immune system;
preparations for detecting genetic predispositions for
medical purposes; reagents for use in medical genetic
testing; reagent paper for medical purposes; diagnostic
preparations for medical purposes; chemical preparations for
medical purposes; enzyme preparations for medical purposes;
preparations for detecting mutation in prion genes for
medical purposes; diagnostic biomarker reagents for medical
purposes. Computer software, recorded; computer software for database
management; data processing apparatus; laboratory devices
for detecting genetic sequences; laboratory equipment,
namely, protein sequence analysis apparatus; DNA
microarrays; nucleic acid sequencers used for analyzing
nucleic acids in scientific research; laboratory apparatus
and instruments, namely, gene analyzers for genome
information; laboratory chemical reactors; laboratory
instrument for the detection of pathogens and toxins in a
biological sample for research use; biochips; DNA chips;
testing apparatus not for medical purposes. Testing apparatus for medical purposes; diagnostic apparatus
for medical purposes; apparatus for use in medical analysis;
apparatus used in implementing diagnosis tests designed to
detect the abnormal prion protein; apparatus for blood
analysis; apparatus for DNA and RNA testing for medical
purposes; medical apparatus and instruments; probes for
medical purposes; genetic testing apparatus for medical
purposes; gas chromatography apparatus for medical purposes.
45.
SAMPLE SORTING STRUCTURE, SAMPLE SORTING METHOD AND RELATED DEVICE
A sample sorting structure (100), comprising: a centrifugal chamber assembly (101), the centrifugal chamber assembly (101) having a centrifugal inner cavity (101a); a rotation driving assembly (102) for driving the centrifugal chamber assembly (101) to rotate; a spindle (103), the spindle (103) being synchronously rotatably connected to the centrifugal chamber assembly (101), and one end of the spindle (103) extending to the centrifugal inner cavity (101a) and being movable relative to the centrifugal inner cavity (101a); an extrusion platform (104) fixed at the end of the spindle (103) extending to the centrifugal inner cavity (101a), the extrusion platform (104) being used for loading a sample bag to be sorted, and is driven by the spindle (103) to move close to or away from the end of the centrifugal inner cavity (101a) used for extruding the sample bag to be sorted; and a horizontal pushing driving assembly (105) for driving the spindle (103) to move in the centrifugal inner cavity (101a), the horizontal pushing driving assembly (105) being rotatably connected to the spindle (103). The sample sorting structure uses an automatic mode of extruding and sorting while centrifuging, improving sample sorting efficiency, making it easier to completely discharge samples, improving sample utilization, and reducing costs. Further disclosed are a sample sorting method based on the sample sorting structure, and a related device.
A sorting fluidic system, a cell sorting system, a sorting instrument, a fluid sorting method and a cell sorting method. The sorting fluidic system comprises a centrifugal mechanism, a pipeline consumable and a pipeline timing control assembly; the centrifugal mechanism is used for holding a sample holding member, a rotary connecting member separately communicated with the sample holding member and the pipeline consumable is mounted on the centrifugal mechanism, and the centrifugal mechanism drives liquid in the sample holding member to achieve centrifugal layering and presses liquid of a corresponding layer into the pipeline consumable; and the pipeline timing control assembly is mounted on the pipeline consumable and used for controlling the liquid entering the pipeline consumable to flow according to preset timing and a preset path. According to the provided sorting fluidic system, a target cell is directly obtained, and a cell contamination risk is reduced. In addition, the sample holding member is pressed by means of the centrifugal machine to press the layers into the pipeline consumable, so that all the layers in the sample holding member are pumped out without independently providing a pump, thereby simplifying the structure of the system.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
A micro-droplet screening device, and a system. The micro-droplet screening device comprises an upper computer (1), an optical signal detection apparatus (2), a signal processor (3), and an electric screener (4). The optical signal detection apparatus (2) is used to acquire an optical signal of a droplet to be measured, and convert the optical signal into an electrical signal of the droplet to be measured. The signal processor (3) is electrically connected to the optical signal detection apparatus (2), the signal processor (3) being used to receive and process the electrical signal of the droplet to be measured, and send a screening instruction to the electrical screener (4) according to the strength of the electrical signal. The electric screener (4) is electrically connected to the signal processor (3), the electric screener (4) controlling the droplet to be measured in a microfluidic sorting chip (5) to deflect to the a corresponding flow channel according to the screening instruction, so as to complete screening of the micro-droplet to be measured. The upper computer (1) is electrically connected to the signal processor (3), the upper computer (1) being used to issue a screening instruction and configure a related screening parameter. Provided is a highly integrated and automated micro-droplet screening device, which greatly improves the convenience of microdroplet screening.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A flexible neural probe (100). The flexible neural probe (100) comprises a flexible probe body and a monitoring electrode mechanism. The flexible probe body comprises an air pressure control layer (200) and an electrode integration layer (300), which are arranged in a stacked manner, wherein at least one air chamber (400) is formed in the air pressure control layer (200). The flexible probe body has a tip; and the monitoring electrode mechanism is arranged on the electrode integration layer (300) and the monitoring electrode mechanism comprises at least one electrode (700), which is located on the tip of the flexible probe body. The air pressure in the air chamber (400) is configured to be adjustable, such that the flexible probe body deforms and changes the monitoring position of the electrode (700).
Provided are a method for constructing a sequencing library, a sequencing library, a sequencing method, and a kit for constructing a sequencing library. The method for constructing a sequencing library comprises: performing, under the action of an exonuclease, a digestion treatment on a sample to be tested which is linked to a linker, so as to obtain a target sequencing library.
Provided in the present invention are a porin monomer, a porin, a mutant thereof and the use thereof. The porin monomer comprises: (a) a protein composed of an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein mutant, wherein the amino acid sequence of the protein mutant is subjected to substitution, deletion and/or addition of one or several amino acids at at least one of the following positions in the amino acid sequence as shown in SEQ ID NO: 1: position 63, position 64, etc., and the protein mutant has the function of forming a pore channel structure via polymerization; or (c) a porin monomer having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99% identity to the protein of (a) or (b), and having the function of forming a pore channel structure via polymerization. By means of the present invention, the problem in the prior art of the poor stability of a pore channel of the porin can be solved; and the present invention is applicable to the field of single-molecule sequencing.
The present disclosure relates to the technical field of spatiotemporal transcriptome data. Disclosed are a batch effect removal model training method and apparatus, and a batch effect removal method and apparatus. The batch effect removal model training method comprises: inputting a gene expression matrix for training into a neural network feature coding module to obtain a first processing result for training; inputting a cell space neighbor graph matrix for training and the first processing result for training into a graph network feature coding module to enhance neighbor center expression, so as to obtain a second processing result for training; splicing the first processing result for training and the second processing result for training to obtain a target gene expression matrix for training; and according to the target gene expression matrix for training, calculating a loss function of a batch effect removal model, and optimizing the batch effect removal model on the basis of the loss function. By comprehensively analyzing gene data of cells and cell space neighbor data, a batch effect between cell data is removed, and distribution of cell data is unified as much as possible, thereby realizing batch effect removal of spatiotemporal transcriptome data.
G06F 30/27 - Optimisation, vérification ou simulation de l’objet conçu utilisant l’apprentissage automatique, p. ex. l’intelligence artificielle, les réseaux neuronaux, les machines à support de vecteur [MSV] ou l’apprentissage d’un modèle
52.
POLYPEPTIDE HAVING DNA POLYMERASE ACTIVITY AND USE THEREOF
Provided are a polypeptide having DNA polymerase activity, the polypeptide comprising an amino acid sequence having at least 80% identity with SEQ ID NO: 1 or a polymerase active fragment or domain thereof, as well as a corresponding isolated nucleic acid, a vector and a host cell comprising the nucleic acid, a method for preparing the polypeptide, a composition or kit comprising the polypeptide or nucleic acid, and a method for nucleic acid amplification and sequencing by means of the polypeptide.
The present disclosure provides a chip quality inspection method and apparatus, an electronic device, and a storage medium. The method comprises: performing numbering processing on at least one field of view in each chip, and obtaining identification information of each field of view; on the basis of the identification information, separately acquiring a first channel image and a second channel image of the at least one field of view; performing image processing analysis on the at least one field of view on the basis of image features of the first channel image and the second channel image; and determining the quality of the chip according to a preset threshold combination and an image processing analysis result. According to embodiments of the present disclosure, a chip is divided into at least one field of view and then processed, and different channel images of each field of view are analyzed, so as to inspect the quality of the chip. In the prior art, parameters of a chip image are recorded mainly by means of manual sampling and observation, while in the present disclosure, a chip flaw can be automatically detected, so that detection accuracy can be improved and standardized detection can be achieved.
The present application relates to the technical field of data processing, and relates to a genotype imputation processing method and apparatus and an electronic device. The method comprises: first acquiring a sequencing file and a graph reference genome set, wherein the graph reference genome set is constructed in advance according to graph genomes, and the graph reference genome set comprises: reference sequences of the genomes and haplotype sequence information for multiple individuals; then calculating a likelihood value of a sequencing read length for each haplotype on the basis of the graph reference genome set; and performing, according to the likelihood value and the graph reference genome set, genotype imputation on the sequencing file that has undergone variant calling. By using the technical solution of the present application, the accuracy and imputation effect of genotype imputation can be improved.
G16B 20/20 - Détection d’allèles ou de variantes, p. ex. détection de polymorphisme d’un seul nucléotide
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
Provided in the present invention are a nanopore sensor and the use thereof in sequencing. The nanopore sensor comprises: a membrane layer and a porin inserted into the middle of the membrane layer to form a pore channel, wherein when an electric field force is applied across the membrane layer, electrical conduction occurs in the pore channel. The porin comprises a porin monomer, and the porin monomer comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 2; or (b) a protein mutant, the amino acid sequence of the protein mutant being subjected to substitution, deletion and/or addition of one or more amino acids at at least one of the following sites of SEQ ID NO: 2: such as 80, 82 and 83, and the mutant having a function of forming a pore channel structure by means of polymerization; or (c) a protein which has at least 70% or above identity to the above-mentioned proteins, the mutant having the function of forming a pore channel structure by means of polymerization. Provided is the novel nanopore sensor capable of being used for nanopore sequencing, which is applicable to the field of single-molecule sequencing.
G01N 27/403 - Ensembles de cellules et d'électrodes
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions
G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
Provided in the present invention are a porin monomer, a porin, a mutant thereof and the use of same. The porin monomer comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein mutant the amino acid sequence of which is obtained by means of substitution, deletion and/or addition of one or several amino acids at at least one of the following sites in SEQ ID NO: 1: the 91st site, the 98th site, the 99th site, etc., the protein mutant having the function of forming pore structures by means of polymerization; or (c) a protein having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99% identity to the protein of (a) or (b), and having the function of forming pore structures by means of polymerization. Provided is a novel nanopore sensor capable of being used for nanopore sequencing, which is applicable to the field of single molecule sequencing.
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
A nanopore sequencing method using a single-stranded specific nuclease, relating to the field of sequencing. The method can improve the sequencing flux, improve the capture efficiency of a target sequencing object, increase the number of holes (channels), increase an average read length of the target sequencing object, and/or improve the porin gating frequency.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
58.
LIBRARY ADAPTER DESIGN FOR IMPROVING SEQUENCING THROUGHPUT
Provided are a method for establishing a sequencing library, a sequencing library, a sequencing method, and a kit for constructing a sequencing library or for sequencing. The method for establishing a sequencing library comprises: digesting, under the action of exonuclease, a sample under test connected with an adapter to obtain a sequencing library. The 5' end of the adapter does not have phosphorylation modification, and the 3' end of the adapter is connected to the 5' end of said sample.
The present invention relates to the field of nanopore sequencing, and in particular to a novel nanopore protein BCP52, and a mutant and use thereof. According to the present invention, a novel nanopore protein BCP52 is found in a deep-sea metagenome, protein preparation and nanopore sequencing verification show that the nanopore protein BCP52 has the capability of being applied to nanopore sequencing, and optimization of the mutant of the nanopore protein BCP52 can improve the accuracy of nanopore sequencing.
Disclosed in the present invention are a helicase and a use thereof. The helicase has two tower domains and a PIN domain, and the two tower domains are located on the same side of a helicase three-dimensional structure. According to the technical solution of the present invention, a brand-new helicase BCH3X having a special helix characteristic domain is provided, and the helicase has good salt tolerance and stability, can have high unwinding activity in the case of a high salt content, can be used for nucleic acid control and characterization, and is applied to nanopore sequencing.
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
Disclosed are a helicase ToPif 1, and a preparation method therefor and a use thereof in high-throughput sequencing. The amino acid sequence of a ToPif 1 helicase mutant is as shown in SEQ ID NO. 1. The helicase can be used for controlling and characterizing a nucleic acid and is applied to nanopore sequencing.
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C07K 14/35 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Mycobacteriaceae (F)
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
Disclosed in the present invention are a mutant of a Dda helicase, and a preparation method therefor and a use thereof in sequencing. The mutant undergoes, on an amino acid sequence as shown in SEQ ID NO: 4, one or more mutations selected from the following: (1) substituting a positively charged amino acid in the amino acid sequence with a neutral amino acid; (2) substituting a negatively charged amino acid in the amino acid sequence with a neutral amino acid; (3) truncating an amino acid in an N-terminal region in the amino acid sequence or substituting the amino acid in the N-terminal region in the amino acid sequence with a short-side-chain neutral amino acid; and (4) substituting, with a short-side-chain amino acid, an amino acid having large steric hindrance in a side-chain amino acid in the amino acid sequence. The mutant greatly improves the sequencing capability, and further promotion of nanopore sequencing work is facilitated.
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
63.
HELICASE AND PREPARATION METHOD THEREFOR AND USE THEREOF IN HIGH-THROUGHPUT SEQUENCING
Provided are a helicase and a preparation method therefor and a use thereof in sequencing. The amino acid sequence of the helicase is as shown by SEQ ID NO:1 or has at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the amino acid sequence as shown by SEQ ID NO:1. The helicase has high thermal stability, ATP hydrolysis activity and DNA unwinding activity, the DNA unwinding activity increases with increasing salt concentration, and the helicase can be used for control and characterization of nucleic acids, and can be applied to nanopore sequencing.
The present disclosure provides a cell image registration method and system, an electronic device, and a storage medium. The cell image registration method comprises: acquiring cell contours in a cellular image and in a spatial transcriptomic data graph; respectively determining specific target cells on the basis of the definition of the cell contours; extracting geometric centers of the specific target cells, to respectively form a two-dimensional point cloud set of the cellular image and a two-dimensional point cloud set of the spatial transcriptomic data graph; then obtaining registration parameters; and registering the cellular image and the spatial transcriptomic data graph according to the registration parameters. According to the present disclosure, cell contours in the cellular image and in the spatial transcriptomic data graph as well as the definition of the cell contours are acquired, then two-dimensional point cloud sets are formed on the basis of the geometric centers of specific target cells, the two-dimensional point cloud sets are processed on the basis of a point cloud registration algorithm, and finally, registration parameters are acquired to register the cellular image and the spatial transcriptomic data graph, thereby overcoming the defect of being unable to perform in-situ analysis, and improving the accuracy of spatial transcriptomic sequencing techniques in spatiotemporal omics technology.
G06V 10/22 - Prétraitement de l’image par la sélection d’une région spécifique contenant ou référençant une formeLocalisation ou traitement de régions spécifiques visant à guider la détection ou la reconnaissance
Provided is a nucleic acid ligase. The nucleic acid ligase contains a mutation sequence with at least 80% identity compared to SEQ ID NO: 1, and the mutation comprises at least one of substitution, deletion, and insertion.
Disclosed in the present invention are a spatiotemporal transcriptome sequencing method, a sequencing library construction method, and a gene sequencing system. The spatiotemporal transcriptome sequencing method of the present invention comprises the following steps: 1) carrying out reverse transcription by means of complementary combination of the 3' end of a probe sequence and the 3' end of template RNA, and adding a template switch oligo sequence at the end of generated cDNA by using reverse transcriptase, the template switch oligo sequence being different from a first adapter sequence at the 5' end of the probe sequence; 2) amplifying the cDNA by using the first adapter sequence and the template switch oligo sequence; and 3) sequencing the cDNA amplified in step 2). By using the method of the present invention, the spatiotemporal transcriptome sequencing time can be shortened, and the 5' end sequencing of a spatiotemporal transcriptome can be achieved.
A microbial species identification method and system, and a device and a medium. The method comprises: determining a microbial species obtained by means of preliminary identification in a sample to be subjected to identification, and a corresponding genus; and according to abundance values of microbial species belonging to the same genus, determining the authenticity of the microbial species obtained by means of preliminary identification. The authenticity of the existence of a microbial species is determined by means of comparison of abundance values of species in a same genus, and for species with relatively low abundance values, the impact of erroneous comparisons of sequencing sequences on an identification result of the microbial species is avoided, thereby improving the accuracy of microbial species identification.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
68.
FINE PURIFICATION METHOD FOR NUCLEIC ACID COMPLEX, FINE PURIFICATION KIT, AND APPLICATION
Provided are a fine purification method for a nucleic acid complex, a fine purification kit, and an application. The nucleic acid complex comprises a polynucleotide fragment and a protein binding to the polynucleotide fragment, or the nucleic acid complex is a polynucleotide fragment of which at least part of the nucleotide is modified to lose the complementary pairing capability of a nucleic acid or nucleic acid analogue. The fine purification method comprises the following steps: S1, immobilizing a nucleic acid or nucleic acid analogue comprising a nucleotide complementary to a protein binding sequence or a modified nucleotide before modification on a polynucleotide fragment, to obtain a solid phase capture element; S2, incubating a nucleic acid complex to be purified and the solid phase capture element, and capturing and removing, by the solid phase capture element and by means of base complementation, a polynucleotide fragment that does not bind to the protein or is not successfully modified, to obtain a purified nucleic acid complex. A linker complex purified by means of sequence complementation is purer than a complex that does not undergo the purification method, and a library constructed by using the complex as a raw material has a better sequencing effect.
An immune repertoire sequencing data analysis method and apparatus, and an electronic device. The analysis method comprises: acquiring sequencing data to be analyzed (S102); filtering said sequencing data on the basis of a germline gene combination database to obtain a first filter sequence (S104), wherein the germline gene combination database is a database constructed on the basis of a recombination result obtained by performing sequence recombination on a germline gene reference sequence; and performing multiple iterative alignments on the first filter sequence to obtain a target molecular sequence corresponding to said sequencing data (S106).
A DNA ligation buffer and a use thereof. The DNA ligation buffer comprises 5-15 mM of divalent metal ions, 0.5-5 mM of DTT, 10-15% of polyethylene glycol, 0.5-5 mM of ATP, and a Tris-HCl buffer, wherein the pH value of the Tris-HCl buffer is 7-9. The DNA ligation buffer can significantly improve the efficiency of a ligation reaction, and increase ligation products in the ligation reaction and the number of molecules capable of being sequenced during library construction.
A method and system for detecting structural variation, a device, and a medium, the method comprising: acquiring sequencing sequences of a sample to be tested, there being a plurality of sequencing sequences; according to single base variation information in each of the sequencing sequences, performing haplotype typing on the sequencing sequences; and assembling sequencing sequences belonging to the same haplotype to obtain a haplotype sequence, and according to the haplotype sequence, performing structural variation analysis. When performing SV detection on the assembled haplotype sequence, since same comprises data from a plurality of sequencing sequences, read length requirements of the SV detection can be met and SV detection results can be more complete and accurate.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G16B 20/30 - Détection de sites de liaison ou de motifs
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Chemical reagents, other than for medical or veterinary
purposes; chemical preparations for scientific purposes,
other than for medical or veterinary use; biochemical
preparations for scientific purposes; biological
preparations, other than for medical or veterinary purposes;
test paper, chemical; biological preparations for use in
cell cultures, other than for medical or veterinary
purposes; chemical substances for analyses in laboratories,
other than for medical or veterinary purposes; chemical
preparations for analyses in laboratories, other than for
medical or veterinary purposes; diagnostic reagents and
preparations, except for medical or veterinary use;
biological tissue cultures, other than for medical or
veterinary purposes. Biological preparations for medical purposes; medical
diagnostic reagents for the analysis of immune system;
preparations for detecting genetic predispositions for
medical purposes; reagents for use in medical genetic
testing; reagent paper for medical purposes; diagnostic
preparations for medical purposes; chemical preparations for
medical purposes; enzyme preparations for medical purposes;
preparations for detecting mutation in prion genes for
medical purposes; diagnostic biomarker reagents for medical
purposes. Computer software, recorded; computer software for database
management; data processing apparatus; laboratory devices
for detecting genetic sequences; laboratory equipment,
namely, protein sequence analysis apparatus; DNA
microarrays; nucleic acid sequencers used for analyzing
nucleic acids in scientific research; laboratory apparatus
and instruments, namely, gene analyzers for genome
information; laboratory chemical reactors; laboratory
instrument for the detection of pathogens and toxins in a
biological sample for research use; biochips; DNA chips;
testing apparatus not for medical purposes. Testing apparatus for medical purposes; diagnostic apparatus
for medical purposes; apparatus for use in medical analysis;
apparatus used in implementing diagnosis tests designed to
detect the abnormal prion protein; apparatus for blood
analysis; apparatus for DNA and RNA testing for medical
purposes; medical apparatus and instruments; probes for
medical purposes; genetic testing apparatus for medical
purposes; gas chromatography apparatus for medical purposes.
A magnetic bead mixing device, comprising a supporting mechanism (100), a driving mechanism (200) and a reagent bottle fixing member (300), wherein the supporting mechanism (100) is provided with an accommodating cavity and an opening; a center line of the accommodating cavity extends in a first straight line (20); an output shaft (201) of the driving mechanism (200) is rotatable around the first straight line (20); the reagent bottle fixing member (300) is configured to fix a reagent bottle (10) containing magnetic beads; the reagent bottle fixing member (300) is arranged in the accommodating cavity by means of the opening; a bottom end of the reagent bottle fixing member (300) is in transmission connection with the output shaft (201); and a central axis of the reagent bottle fixing member (300) extends in a second straight line (30). The reagent bottle fixing member (300) can swing in such a way that the second straight line (30) circumferentially moves around the first straight line (20) under the driving of the driving mechanism (200), and during swinging, an included angle between the second straight line (30) and the first straight line (20) is always α°, and a moving track of the second straight line (30) is a conical surface.
A method for forming a micro-pattern array on a receiving substrate using a hydrogel stamp, comprising: providing a hydrogel stamp, wherein a micro-pattern array is provided at the bottom of the hydrogel stamp; loading a molecular solution into the hydrogel stamp; and under the action of an electric field or a current, imprinting molecules on a receiving substrate to obtain the micro-pattern array. The hydrogel stamp is used under the action of the electric field or the current, so that the hydrogel stamp can be used for repeated imprinting. The imprinting efficiency is high, and there is no need for multiple times of infiltration, so that time is saved. and a cost is reduced. In addition, no additional chemical reagents and complex processes are needed, the operation is simple, and the biocompatibility of a system is excellent, so that molecular loss can be effectively reduced, and cross contamination in a molecular transfer printing process is avoided.
Provided are an in situ sequencing library construction method, an in situ sequencing method, and a use. The construction method comprises: placing a sample on a solid support having an RNA capture function, and then performing non-cross-linking fixation and capture on mRNA in the tissue sample or cell sample; reverse transcribing the captured mRNA into cDNA, and removing tissue residues in the reverse transcribed tissue sample or cell residues in the reverse transcribed cell sample, to obtain a solid support having undergone impurity removal, the cDNA being fixed on the solid support having undergone impurity removal; and performing target capture and amplification on the cDNA on the solid support having undergone impurity removal, to obtain an amplification product serving as an in-situ sequencing library. A capture probe is designed on the solid support to bind to the mRNA in the sample, to fix the mRNA on the solid support, and a chemical cross-linking reagent does not need to be used to fix the mRNA in the sample, thereby avoiding the problem of low reverse transcription efficiency caused by a cross-linking fixation operation.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
The present invention provides a helicase, a preparation method therefor, and the use thereof in high-throughput sequencing. The amino acid sequence of the helicase is as shown in SEQ ID NO: 1, or has at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% identity to the amino acid sequence as shown in SEQ ID NO: 1. The helicase still has high unwinding activity in a high-salt environment, can be used for control and characterization of a nucleic acid, and is applied to nanopore sequencing.
An uncoupling reagent composition, a kit, a continuous sequencing method and the use. The uncoupling reagent composition comprises cyclodextrin and a nuclease. The method can solve the problems in the prior art of the large charging amount of an uncoupling reagent, the poor uncoupling effect, and cross contamination caused by sample residues; and is suitable for the field of high-throughput sequencing.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
78.
PIN DOMAIN, HELICASE CONTAINING SAME, PREPARATION METHOD FOR PIN DOMAIN, AND USE OF PIN DOMAIN
The present invention provides a pin domain, a helicase containing same, a preparation method for the pin domain, and a use of the pin domain. The amino acid sequence of the pin domain contains at least 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53 or 54 amino acids. The helicase containing the pin domain still has high unwinding activity in a high-salt environment, can be used for nucleic acid control and characterization, and is applied to nanopore sequencing.
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
Disclosed are a nanopore protein and an application thereof. The invention provides a pore protein which is (a), (b), or (c), below: a) a protein having an amino acid sequence such as that shown in SEQ ID NO: 1; b) a protein having 70% or more identity to SEQ ID NO: 1 and having the functions of SEQ ID NO: 1; c) a fusion protein obtained by adding a tag to a terminal of the protein sequence shown in a) or b). The present invention utilizes a gene mining means predicted by a computer assisted structure so as to mine from a deep-sea metagenome a nanopore protein BCP56, and by means of protein preparation and nanopore sequencing validation shows the applicability thereof to nanopore sequencing, allowing for detecting small molecules, DNA, RNA, polypeptides, etc., and by optimizing mutants thereof, having the potential to improve the accuracy of nanopore sequencing.
Provided is a nanopore protein BCP22, which is (a), (b) or (c): a) a protein having an amino acid sequence as shown in SEQ ID NO: 1; b) a protein having more than 70% identity to SEQ ID NO: 1 and having the function of SEQ ID NO: 1; and c) a fusion protein obtained by adding a tag to the end of the protein sequence as shown in a) or b), and can be applied to nanopore sequencing for detecting small molecules, DNA, RNA, polypeptides and the like.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
A method for stitching a plurality of images. The method comprises: separately extracting feature points in each of a plurality of images; generating an adjacency list for each of the plurality of images on the basis of the extracted feature points, wherein the adjacency list indicates a spatial position relationship between a corresponding image and an adjacent image thereof; traversing adjacency lists for the plurality of images, so as to determine a spatial position relationship between the plurality of images; and stitching the plurality of images on the basis of the determined spatial position relationship between the plurality of images.
A data correction method and system based on a Gaussian smoothing model, a related computer device, and a storage medium. The data correction method comprises: acquiring gene expression data and position data of a plurality of samples; processing nearest-neighbor samples of the plurality of samples, wherein nearest-neighbor samples of an individual sample are determined according to the gene expression data of the plurality of samples, and the weight of the corresponding nearest-neighbor samples of the individual sample is calculated according to position data of the individual sample and position data of the corresponding nearest-neighbor samples; and calculating a correction value of gene expression data of the individual sample according to gene expression data and the weight of the corresponding nearest-neighbor sample of the individual sample. The data correction method of the present invention can solve the problems of high noise and high sparsity in data, and has the advantage of enhancing features, thereby reducing noise interference and providing reliable data for downstream analysis.
Disclosed in the present application are a biological sample quantification method, an apparatus, a device, and a medium. The present application relates to the technical field of biology. The method comprises: acquiring the Nth sequencing result of sequencing of a biological sample to be tested, N being an integer greater than 0; if a biomolecule in the Nth sequencing result is a biological segment to be quantified, determining a theoretical content of said biological segment in said biological sample according to the sequencing starting time of said biological sample, the generation time of the Nth sequencing result, the total length of the biomolecule from a first sequencing result to the Nth sequencing result, and the sequencing rate coefficient, capture rate coefficient, and effective nanopore number of sequencing of said biological sample; and using the theoretical content as an actual content of said biological segment in said biological sample. The method can improve the quantification efficiency of biological samples.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
84.
DETECTION COMPOSITION AND PROTEIN CAPTURE DETECTION METHOD
The present invention relates to the field of biotechnology, and in particular to a detection composition and a protein capture detection method. Provided is a detection composition, comprising a nucleic acid array probe and a protein-nucleic acid conjugate, wherein the nucleic acid array probe comprises a capture sequence, and the capture sequence is used for capturing the protein-nucleic acid conjugate. Simultaneous detection of ultra-high multiplex antibodies can be performed on a same tissue slice. Because spatial reduction of the antibody position depends on sequencing technology, the background staining problem caused by traditional immunohistochemistry and immunofluorescence color developing agents can be solved. In addition, negative control, positive control, and detection of an antibody under test can be carried out on the same tissue slice, a detection result can be determined more accurately, and a false-negative or false-positive result caused by traditional staining technology is eliminated.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
G01N 31/00 - Recherche ou analyse des matériaux non biologiques par l'emploi des procédés chimiques spécifiés dans les sous-groupesAppareils spécialement adaptés à de tels procédés
85.
PORIN MONOMER, PORIN, MUTANT THEREOF, AND USE THEREOF
The present invention provides a porin monomer, a porin, a mutant thereof, and a use thereof. The porin monomer comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO:1; or (b) a protein mutant, wherein the amino acid sequence of the protein mutant is obtained by performing substitution, deletion and/or addition of one or more amino acids at at least one of the following positions in SEQ ID NO:1: positions 77, 81, 82, etc., wherein the mutant has a function of forming a pore channel structure by means of polymerization; or (c) a protein having at least 70%, 75%, 80%, 85%, 90%, 95%, or 99% identity to the protein in (a) or (b), wherein the mutant has a function of forming a pore channel structure by means of polymerization. The present invention can solve the problem in the prior art of a low capture rate of a nano porin to a library, and is applicable to the field of single-molecule sequencing.
A detection apparatus and a sample analyzer. The detection apparatus comprises: a coil module (100), which comprises a transmitting coil (111) and a receiving coil (112); a driving and detection module (200), which is connected to the coil module (100), and is configured to drive the transmitting coil (111) to generate a magnetic field and detect a magnetic field signal received by the receiving coil (112); a signal processing module (300), which is connected to the driving and detection module (200), and is configured to perform conversion, amplification and conditioning on the detected magnetic field signal; and a controller (400), which is connected to the signal processing module (300), and is configured to parse the magnetic field signal received from the signal processing module (300) and upload data related to the magnetic field signal, which data is obtained by means of parsing, wherein the coil module (100) further comprises a coil circuit board (110), and the transmitting coil (111) and the receiving coil (112) are both drawn on the coil circuit board (110).
G01N 15/00 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux
87.
NOVEL PORIN BCP34, AND MUTANT THEREOF AND USE THEREOF
The present invention provides a novel porin BCP34, and a mutant thereof and a use thereof. Specifically, the wild-type porin BCP34 is a nonamer protein, and has a monomer of which the amino acid sequence is SEQ ID NO: 1; and the mutant of the monomer of the porin BCP34 comprises an amino acid sequence having at least 70%, 75%, 80%, 85%, 90%, 95%, or at least 99% identity to SEQ ID NO: 1, and an amino acid sequence having mutations at one or more positions in a sensor region, a transmembrane region, the entrance, barrel inner wall, and the exit of the monomer of the wild-type porin BCP34. The wild-type porin BCP34 of the present invention has the capability of being applied to nanopore sequencing, and the mutant of the porin BCP34 obtained by optimization has higher library capture capability and nanopore sequencing accuracy.
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
A61K 47/42 - ProtéinesPolypeptidesLeurs produits de dégradationLeurs dérivés p. ex. albumine, gélatine ou zéine
Provided are an antibody, a nucleic acid encoding same, a vector comprising the nucleic acid, a host cell, a kit, a method for preparing a hot-start chimeric DNA polymerase or a variant thereof, a hot-start chimeric DNA polymerase or a variant thereof, and an amplification method. The antibody can specifically neutralize a chimeric DNA polymerase or a variant thereof, and the hot-start chimeric DNA polymerase prepared using the antibody performs excellently in conventional PCR amplification and room temperature PCR amplification systems.
Provided in the present disclosure is a spatiotemporal transcriptomic sequencing method, comprising: determining the cDNA abundance of a sample to be sequenced; and performing a spatiotemporal transcriptomic sequencing step on said sample on the basis of the cDNA abundance, wherein determining the cDNA abundance of said sample comprising a pre-spatiotemporal transcriptomic sequencing step. According to the specific embodiments of the present disclosure, the cDNA abundance obtained by means of the method can accurately reflect the gene capture capability of the spatiotemporal transcriptomic technology on tissue slices. Compared with the prior art, the method omits steps of cDNA recovery and cDNA shearing, thereby greatly saving the operation time.
The present invention provides a biological nanopore sensor and a preparation method therefor. The method for preparing the biological nanopore sensor comprises: a) preparing a hydrophilic protein into a liposome; and b) mixing the liposome with a membrane layer, and promoting the liposome to be inserted into the membrane layer to obtain the biological nanopore sensor, wherein the hydrophilic protein is provided with a nanochannel. The method of the present invention can solve the problem in the prior art that it is difficult to prepare the hydrophilic protein into a biological nanopore sensor, and is applied to the field of nanopore sequencing.
G01N 27/49 - Systèmes impliquant la détermination du courant à une valeur unique spécifique, ou dans une petite plage de valeurs, pour une tension appliquée afin de produire la mesure sélective d'une ou plusieurs espèces ioniques particulières
91.
SEQUENCING ADAPTER, SEQUENCING ADAPTER COMPLEX, AND METHOD FOR MULTIPLEX NANOPORE SEQUENCING OF TARGET NUCLEIC ACID SEQUENCE
Provided are a sequencing adapter, a sequencing adapter complex, and a method for multiplex nanopore sequencing of a target nucleic acid sequence. The sequencing adapter comprises a first strand and a second strand. The first strand comprises, sequentially from a 5' end to a 3' end, a sequencing guide sequence, a helicase binding sequence, a spacer structure, a first complementary sequence, and a first primer sequence. The spacer structure prevents a helicase from moving, and the first strand has a 3' free end. The second strand comprises, sequentially from a 5' end to a 3' end, a second complementary sequence and a constraining sequence or a sequence complementary to the nucleic acid sequence of the constraining sequence. The constraining sequence comprises a nucleic acid sequence having a hydrophobic molecule linked to an end thereof. The first complementary sequence is reverse complementary to the second complementary sequence, and the sequencing adapter is formed by annealing the first and second strands. The construction process of the sequencing adapter and the sequencing adapter complex is simple and has a high yield.
Disclosed in the present invention are a sequencing linker, a sequencing linker complex, a method for multiple amplifications of a target nucleic acid sequence, and a method for nanopore sequencing. The sequencing linker comprises a first strand and a second strand. The first strand sequentially comprises, from the 5' end to the 3' end, a sequencing guide sequence, a helicase binding sequence, a position limiting structure, a first complementary sequence and a first primer sequence, wherein the position limiting structure prevents the movement of a helicase, and the first strand has a free 3' end. The second strand sequentially comprises, from the 5' end to the 3' end, a second complementary sequence, and a constraining sequence or a sequence complementary to the nucleic acid sequence of the constraining sequence, wherein the constraining sequence comprises a nucleic acid sequence with a hydrophobic molecule connected to the end. The first complementary sequence is reversely complementary to the second complementary sequence, and the sequencing linker is formed by means of annealing the first strand and the second strand. The sequencing linker further comprises a structure that prevents the second strand from being displaced by polymerases. The sequencing linker and sequencing linker complex construction process of the present invention is simple and has a high yield.
The present application provides a microfluidic sorting chip, a droplet screening method, a system, a device, and a storage medium, and belongs to the technical field of microfluidics. Wherein the droplet screening method comprises: according to a preset frequency, acquiring at least two types of electrical signals of a droplet to be detected; determining within the electrical signals a target electrical signal which satisfies an intensity threshold; when the signal width of the target electrical signal satisfies a signal width threshold, extracting features from target electrical signal data, judging whether in-channel features of the target electrical signal satisfy a first preset condition, and judging whether inter-channel features of the target electrical signal satisfy a second preset condition; and if so, then judging that the droplet to be detected is a target droplet. The in-channel features are the features of the droplet to be detected which correspond to in-channel features of each analog-to-digital conversion signal channel, and the inter-channel features are the features of the droplet to be detected which correspond to inter-channel features of each analog-to-digital conversion signal channel. The present application provides diverse screening solutions for screening droplets to be detected, thereby enhancing the flexibility and accuracy of screening droplets to be detected.
Provided are a DNA helicase BCH516 and a mutant thereof. The amino acid sequence of the helicase is as shown in SEQ ID NO: 1, and a nucleotide sequence for encoding the helicase is as shown in SEQ ID NO: 2. The helicase has salt tolerance and stability, and has high soluble expression quantity, and thus can be applied to nucleic acid control and characterization and nanopore sequencing.
The present application provides a film forming structure and a forming method. The forming structure comprises: a support body, the inside of the support body being filled with a first buffer solution, the surface of the first buffer solution being provided with a membrane solution, the surface of the membrane solution being provided with a second buffer solution, and the osmotic pressure of the first buffer solution being greater than the osmotic pressure of the second buffer solution. The forming structure and the forming method can quickly respond to a supported object having a specific structure, increasing the operable window thereof, achieving controllability and uniformity, shortening the film forming period, and saving corresponding costs.
Provided are a correction method and apparatus for gene sequencing, and an electronic device, which relate to the technical field of biological information. The method comprises: first, performing multiple instances of sequencing on the same DNA molecular template, so as to obtain a base value and a quality value at a target position that correspond to each instance of sequencing; then, on the basis of the base value and the quality value which correspond to each instance of sequencing, respectively determining a correction error rate for each type of base at the target position; next, according to the correction error rate, respectively determining a correction quality value for each type of base at the target position; and then according to the correction quality value, determining a corresponding corrected base value at the target position. By means of applying the technical solution, a correction solution of performing multiple instances of repeated sequencing is used, such that the accuracy of sequencing can be improved; and the technical solution of performing multiple instances of sequencing can be applied on the basis of a BGIseq sequencing platform, such that the accuracy of sequencing, especially the accuracy of sequencing short-fragment DNA, can be further improved on the basis of the already excellent accuracy of sequencing using the BGIseq platform.
Provided in the present application are a quality testing method and apparatus for a spatiotemporal omics positioning chip, and a device and a storage medium. The method comprises: obtaining a sequencing result of a chip under test, and generating a base quality distribution map of said chip according to the sequencing result of said chip; performing quality testing on said chip according to the base quality distribution map, so as to obtain a quality indicator of said chip, wherein the quality indicator comprises at least one of the proportion of low-quality sequences, the maximum bright-spot area, the number of bright spots, the standard deviation of bright-spot areas, and the total area of bright spots; and determining a quality testing result of said chip according to the quality indicator of said chip and a preset quality indicator threshold value. In the present solution, various quality indicators of a chip under test are obtained by means of analyzing a base quality distribution map of said chip, and a quality testing result is then determined according to the quality indicators. It is obvious that a quality testing result obtained by means of image analysis has higher accuracy than a result obtained by means of naked-eye recognition, and the testing efficiency of such a testing method is also higher.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
98.
SUPER-RESOLUTION RECONSTRUCTION METHOD AND APPARATUS BASED ON DEEP LEARNING, AND ELECTRONIC DEVICE
Disclosed in the present invention are a super-resolution reconstruction method and apparatus based on deep learning. The method comprises: obtaining several pictures to be reconstructed; and on the basis of a trained deep learning network model, reconstructing the pictures to be reconstructed, so as to obtain reconstructed pictures, wherein the resolutions of the reconstructed pictures are higher than the resolutions of the pictures to be reconstructed, the deep learning network model is implemented on the basis of an RCAN, and training data of the deep learning network model is obtained on the basis of simulation. By means of the implementation scheme, high-quality true value images generated on the basis of simulation are used as a training set for deep learning, such that the problem of it being difficult to obtain an existing high-quality deep learning training set by means of experiments is solved, and the quality of reconstructed pictures can also be effectively guaranteed.
Disclose in the present invention are a splint sequence and a use thereof in nucleic acid capture. The first base at the 5' end of the splint sequence is U. During nucleic acid capture, by using the splint sequence provided by the present invention, the effect of effectively reducing the number of adapters generated by the splint sequence in a sequencing library can be achieved, the mapping rate of sequencing data can be improved, and the sequencing costs can be reduced.
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
An improved nucleic acid capture method, comprising the following steps: by means of a splint oligonucleotide hybridization random probe and an oligonucleotide strand fixed on a capture chip, an RNA in a sample captured by the random probe undergoing reverse transcription into a cDNA, and linking the cDNA to the oligonucleotide strand, the reverse transcription and the linking being carried out in the same reaction system. During the process, while the RNA undergoes the reverse transcription into the cDNA, the cDNA is linked, by means of splint hybridization of fixed oligonucleotide sequences at two ends, to the oligonucleotide strand fixed on the chip. The method can increase the number of captured genes, and reduce the loss of cDNA; in addition, the method combines what was originally three steps into one step, thus further shortening the duration of the process.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
