Disclosed are microfluidic devices. The microfluidic device comprises microfeatures disposed in the loading conduit thereof, configured to separate impurities from a fluid flowing from the loading conduit to the sample compartments. The loading conduit comprises a filtration section comprising the microfeatures, and a narrow section in fluidic communication with the filtration section. The filtration section has a larger cross-sectional area than the cross-sectional area of the narrow section, resulting in added filtration capacity for the loading conduit of the microfluidic device.
Methods and systems are disclosed for quantifying one or more target concentrations in a biological sample using an analyte detection apparatus configured to analyze an array of partitions of the biological sample. A disclosed method comprises calculating expected locations of partitions in a representation of the array of partitions such as an image, based on corner locations of the array of partitions and analyzing images representing partitions associated with the expected locations of the partitions. The method further comprises determining observed locations of the partitions based on an analysis result of the images and quantifying the one or more target concentrations in the biological sample based on the observed locations of the partitions. These and other methods and systems are disclosed herein.
G06T 7/32 - Détermination des paramètres de transformation pour l'alignement des images, c.-à-d. recalage des images utilisant des procédés basés sur la corrélation
G06T 7/33 - Détermination des paramètres de transformation pour l'alignement des images, c.-à-d. recalage des images utilisant des procédés basés sur les caractéristiques
G06T 7/73 - Détermination de la position ou de l'orientation des objets ou des caméras utilisant des procédés basés sur les caractéristiques
G06V 10/764 - Dispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant la classification, p. ex. des objets vidéo
G06V 10/774 - Génération d'ensembles de motifs de formationTraitement des caractéristiques d’images ou de vidéos dans les espaces de caractéristiquesDispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant l’intégration et la réduction de données, p. ex. analyse en composantes principales [PCA] ou analyse en composantes indépendantes [ ICA] ou cartes auto-organisatrices [SOM]Séparation aveugle de source méthodes de Bootstrap, p. ex. "bagging” ou “boosting”
G06V 10/82 - Dispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant les réseaux neuronaux
G16B 25/20 - Réaction en chaîne par polyméraseConception d’amorces ou de sondesOptimisation de la sonde
4.
METHODS AND SYSTEMS FOR NUCLEIC ACID ANALYSIS ANDQUANTIFICATION
The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules
A microfluidic device for processing a sample comprises an inlet port fluidically coupled to a closed system is disclosed. The closed system comprises a plurality of linear loading conduits, a plurality of terminating chambers, and pluralities of microchambers for receiving the sample. Also disclosed are methods for drawing sample amounts into microchambers of the microfluidic device. One method includes a step of applying a plurality of pressure pulses to contents within the microfluidic assemblies, the plurality of pressure pulses comprising a first pressure applied for a first time interval and a second pressure applied for a second time interval, wherein a volume of the sample is drawn into a plurality of microchambers of the microfluidic device. These and other embodiments of microfluidic devices and methods are disclosed.
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
The present disclosure provides devices and methods for partitioning samples and analyzing analytes. The device may comprise one or more of a first plurality of first chambers and a second plurality of second chambers. A first chamber of the first plurality of chambers may have a first volume that is different from a second volume of a second chamber of the second plurality of chambers. The first plurality of chambers may comprise at least about 100 first chambers and the second plurality of chambers may comprise at least about 100 second chambers.
Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.
The present disclosure provides methods and systems for nucleic acid identification. Identification of a nucleic acid molecule may include generating, in a plurality of chambers, a plurality of double-stranded nucleic acid molecules, denaturing the double-stranded nucleic acid molecules, and detecting signals of the denaturation to generate one or more denaturation profiles. The one or more denaturation profiles may be usable to identify nucleic acid molecules. The methods and system described herein may provide for identification of multiple nucleic acid molecules from a single analysis.
A microfluidic device for processing a sample comprises an inlet port fluidically coupled to a closed system is disclosed. The closed system comprises a plurality of linear loading conduits, a plurality of terminating chambers, and pluralities of microchambers for receiving the sample. Also disclosed are methods for drawing sample amounts into microchambers of the microfluidic device. One method includes a step of applying a plurahty of pressure pulses to contents within the microfluidic assemblies, the plurahty of pressure pulses comprising a first pressure applied for a first time interval and a second pressure applied for a second time interval, wherein a volume of the sample is drawn into a plurality of microchambers of the microfluidic device. These and other embodiments of microfluidic devices and methods are disclosed.
Methods and systems are disclosed for quantifying one or more target concentrations in a biological sample using an analyte detection apparatus configured to analyze an array of partitions of the biological sample. A disclosed method comprises calculating expected locations of partitions in a representation of the array of partitions such as an image, based on corner locations of the array of partitions and analyzing images representing partitions associated with the expected locations of the partitions. The method further comprises determining observed locations of the partitions based on an analysis result of the images and quantifying the one or more target concentrations in the biological sample based on the observed locations of the partitions. These and other methods and systems are disclosed herein.
The present disclose provides methods for forming a microfluidic device. Methods for forming a microfluidic device may comprise providing a microfluidic structure and a film, treating a surface of the microfluidic structure, a surface of the film, or both with a solvent, subsequently pressing the microfluidic structure together with the film under a first heating condition to form the microfluidic device comprising the solvent, and applying a negative pressure to the microfluidic device under a second heating condition, which negative pressure is applied for a time period greater than 30 minutes or at a pressure less than 20 kilopascals (kPa) to remove at least a portion of the solvent. In some aspects, the present disclosure provides devices consistent with the methods herein.
The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
The present disclosure provides methods and systems for analysis of an analyte. Analysis of an analyte may include contacting an analyte with a binding reagent, which binding reagent may include a binding probe with specificity for the analyte and a nucleic acid molecule. The nucleic acid molecule may be denatured and signals indicative of the denaturing of the analyte may be collected. The collected signals may be used to detect, identify, or quantify the analyte.
The present disclosure provides devices and methods for partitioning samples and analyzing analytes. The device may comprise one or more of a first plurality of first chambers and a second plurality of second chambers. A first chamber of the first plurality of chambers may have a first volume that is different from a second volume of a second chamber of the second plurality of chambers. The first plurality of chambers may comprise at least about 100 first chambers and the second plurality of chambers may comprise at least about 100 second chambers.
The present disclosure provides methods and systems for analysis of an analyte. Analysis of an analyte may include contacting an analyte with a binding reagent, which binding reagent may include a binding probe with specificity for the analyte and a nucleic acid molecule. The nucleic acid molecule may be denatured and signals indicative of the denaturing of the analyte may be collected. The collected signals may be used to detect, identify, or quantify the analyte.
The present disclosure provides devices and methods for partitioning samples and analyzing analytes. The device may comprise one or more of a first plurality of first chambers and a second plurality of second chambers. A first chamber of the first plurality of chambers may have a first volume that is different from a second volume of a second chamber of the second plurality of chambers. The first plurality of chambers may comprise at least about 100 first chambers and the second plurality of chambers may comprise at least about 100 second chambers.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Kits consisting primarily of reagents and laboratory
equipment, namely, microfluidic array plates; laboratory
reagents for scientific and research use, namely,
oligonucleotides, primers and probes, enzymes and enzyme
mixes, nucleotide sets and nucleotide mixes, reference dye,
reaction buffers, detection label, sold individually or as a
kit, for nucleic acid genotyping, nucleic acid
quantification and nucleic acid amplification including
polymerase chain reaction and reverse transcription
polymerase chain reaction (term considered too vague by the
International Bureau - Rule 13 (2) (b) of the Regulations). Kits consisting primarily of reagents and laboratory
equipment, namely, microfluidic array plates;
oligonucleotides, primers and probes, enzymes and enzyme
mixes, nucleotide sets and nucleotide mixes, reference dye,
reaction buffers, detection label, sold individually or as a
kit, for nucleic acid genotyping, nucleic acid
quantification and nucleic acid amplification including
polymerase chain reaction and reverse transcription
polymerase chain reaction for use in clinical laboratories,
hospitals, point-of-care and direct consumer applications
(term considered too vague by the International Bureau -
Rule 13 (2) (b) of the Regulations). Laboratory equipment, namely, microfluidic array plates and
optical detection apparatus (term considered too vague by
the International Bureau - Rule 13 (2) (b) of the
Regulations).
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Kits consisting primarily of reagents and laboratory
equipment, namely, microfluidic array plates; laboratory
reagents for scientific and research use, namely,
oligonucleotides, primers and probes, enzymes and enzyme
mixes, nucleotide sets and nucleotide mixes, reference dye,
reaction buffers, detection label, sold individually or as a
kit, for nucleic acid genotyping, nucleic acid
quantification and nucleic acid amplification including
polymerase chain reaction and reverse transcription
polymerase chain reaction (term considered too vague by the
International Bureau - Rule 13 (2) (b) of the Regulations). Kits consisting primarily of reagents and laboratory
equipment, namely, microfluidic array plates;
oligonucleotides, primers and probes, enzymes and enzyme
mixes, nucleotide sets and nucleotide mixes, reference dye,
reaction buffers, detection label, sold individually or as a
kit, for nucleic acid genotyping, nucleic acid
quantification and nucleic acid amplification including
polymerase chain reaction and reverse transcription
polymerase chain reaction for use in clinical laboratories,
hospitals, point-of-care and direct consumer applications
(term considered too vague by the International Bureau -
Rule 13 (2) (b) of the Regulations). Laboratory equipment, namely, microfluidic array plates and
optical detection apparatus.
The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a first channel, at least one chamber, and a second channel. The first channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel. The second channel may be configured for pressurized outgassing of the chamber, first channel, or both the chamber and first channel.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01F 33/302 - Micromixeurs les matières à mélanger s'écoulant sous forme de gouttelettes
B01F 33/3033 - Micromixeurs utilisant la chaleur pour mélanger ou déplacer les fluides
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
G01N 21/29 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection visuelle
The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a first channel, at least one chamber, and a second channel. The first channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel. The second channel may be configured for pressurized outgassing of the chamber, first channel, or both the chamber and first channel.
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Kits consisting primarily of laboratory and scientific reagents and laboratory equipment being microfluidic array plates for laboratory and scientific research use, other than for medical or veterinary use; laboratory reagents for scientific and research use, namely, oligonucleotides, DNA primers and biochemical probes for detecting and analyzing molecules in protein or nucleotide arrays, enzymes and enzyme mix substrates, nucleotide sets and nucleotide mixes both consisting primarily of nucleotides for scientific and research use, fluorescent reference dye, reaction buffers, sold individually and also sold together as a kit, for nucleic acid genotyping, nucleic acid quantification and nucleic acid amplification, and polymerase chain reaction and reverse transcription polymerase chain reaction, all of the foregoing for laboratory, scientific and research use.
(2) Laboratory equipment, namely, microfluidic array plates and optical detection apparatus in the nature of a laboratory instrument for the detection of nucleic acid molecules in a biological sample for research use.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Kits consisting primarily of reagents and laboratory equipment, namely, microfluidic array plates; laboratory reagents for scientific and research use, namely, oligonucleotides, primers and probes, enzymes and enzyme mixes, nucleotide sets and nucleotide mixes, reference dye, reaction buffers, detection label, sold individually or as a kit, for nucleic acid genotyping, nucleic acid quantification and nucleic acid amplification including polymerase chain reaction and reverse transcription polymerase chain reaction.
(2) Kits consisting primarily of reagents and laboratory equipment, namely, microfluidic array plates; oligonucleotides, primers and probes, enzymes and enzyme mixes, nucleotide sets and nucleotide mixes, reference dye, reaction buffers, detection label, sold individually or as a kit, for nucleic acid genotyping, nucleic acid quantification and nucleic acid amplification including polymerase chain reaction and reverse transcription polymerase chain reaction for use in clinical laboratories, hospitals, point-of-care and direct consumer applications.
(3) Laboratory equipment, namely, microfluidic array plates and optical detection apparatus.
27.
SYSTEMS AND METHODS FOR ANALYZING A BIOLOGICAL SAMPLE
The present disclosure provides methods and systems for nucleic acid identification. Identification of a nucleic acid molecule may include generating, in a plurality of chambers, a plurality of double-stranded nucleic acid molecules, denaturing the double-stranded nucleic acid molecules, and detecting signals of the denaturation to generate one or more denaturation profiles. The one or more denaturation profiles may be usable to identify nucleic acid molecules. The methods and system described herein may provide for identification of multiple nucleic acid molecules from a single analysis.
The present disclosure provides methods and systems for nucleic acid identification. Identification of a nucleic acid molecule may include generating, in a plurality of chambers, a plurality of double-stranded nucleic acid molecules, denaturing the double-stranded nucleic acid molecules, and detecting signals of the denaturation to generate one or more denaturation profiles. The one or more denaturation profiles may be usable to identify nucleic acid molecules. The methods and system described herein may provide for identification of multiple nucleic acid molecules from a single analysis.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Kits consisting primarily of laboratory and scientific reagents and laboratory equipment being microfluidic array plates for laboratory and scientific research use, other than for medical or veterinary use; laboratory reagents for scientific and research use, namely, oligonucleotides, DNA primers and biochemical probes for detecting and analyzing molecules in protein or nucleotide arrays, enzymes and enzyme mix substrates, nucleotide sets and nucleotide mixes both consisting primarily of nucleotides for scientific and research use, fluorescent reference dye, reaction buffers, sold individually and also sold together as a kit, for nucleic acid genotyping, nucleic acid quantification and nucleic acid amplification, and polymerase chain reaction and reverse transcription polymerase chain reaction, all of the foregoing for laboratory, scientific and research use Laboratory equipment, namely, microfluidic array plates and optical detection apparatus in the nature of a laboratory instrument for the detection of nucleic acid molecules in a biological sample for research use
30.
METHODS AND SYSTEMS FOR MICROFLUIDIC DEVICE MANUFACTURING
The present disclose provides methods for forming a microfluidic device. Methods for forming a microfluidic device may comprise providing a microfluidic structure and a film, treating a surface of the microfluidic structure, a surface of the film, or both with a solvent, subsequently pressing the microfluidic structure together with the film under a first heating condition to form the microfluidic device comprising the solvent, and applying a negative pressure to the microfluidic device under a second heating condition, which negative pressure is applied for a time period greater than 30 minutes or at a pressure less than 20 kilopascals (kPa) to remove at least a portion of the solvent. In some aspects, the present disclosure provides devices consistent with the methods herein.
The present disclose provides methods for forming a microfluidic device. Methods for forming a microfluidic device may comprise providing a microfluidic structure and a film, treating a surface of the microfluidic structure, a surface of the film, or both with a solvent, subsequently pressing the microfluidic structure together with the film under a first heating condition to form the microfluidic device comprising the solvent, and applying a negative pressure to the microfluidic device under a second heating condition, which negative pressure is applied for a time period greater than 30 minutes or at a pressure less than 20 kilopascals (kPa) to remove at least a portion of the solvent. In some aspects, the present disclosure provides devices consistent with the methods herein.
The present disclose provides devices, methods and systems that may be used for amplifying and quantifying nucleic acid molecules. Methods for amplifying and quantifying nucleic acids may comprise isolating a sample comprising nucleic acid molecules into a plurality of chambers, performing a polymerase chain reaction on the plurality of chambers, and analyzing the results of the polymerase chain reaction.
Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.
Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.
Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0.degree. and less than 90.degree. to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.
The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a fluid flow path and a chamber. The fluid flow path may comprise a channel and an inlet port and no outlet port. The inlet port may be configured to direct a biological sample to the channel. The channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel and retain the biological sample during processing.
The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Polymerase chain reaction (PCR) instruments for use in scientific research, namely, laboratory apparatus and instruments for amplifying DNA using polymerase chain reaction (PCR); scientific research instruments for use in measuring and quantifying copy number of DNA. Medical devices for detection of cancer; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (PCR) platform.
The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a fluid flow path and a chamber. The fluid flow path may comprise a channel and an inlet port and no outlet port. The inlet port may be configured to direct a biological sample to the channel. The channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel and retain the biological sample during processing.
The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a fluid flow path and a chamber. The fluid flow path may comprise a channel and an inlet port and no outlet port. The inlet port may be configured to direct a biological sample to the channel. The channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel and retain the biological sample during processing.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Polymerase chain reaction (PCR) instruments for use in scientific research, namely, laboratory apparatus and instruments for amplifying DNA using polymerase chain reaction (PCR); scientific research instruments for use in measuring and quantifying copy number of DNA Medical devices for detection of cancer; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (PCR) platform
42.
MICROFLUIDIC SIPHONING ARRAY FOR NUCLEIC ACID QUANTIFICATION
The present disclose provides devices, methods and systems that may be used for amplifying and quantifying nucleic acid molecules. Methods for amplifying and quantifying nucleic acids may comprise isolating a sample comprising nucleic acid molecules into a plurality of chambers, performing a polymerase chain reaction on the plurality of chambers, and analyzing the results of the polymerase chain reaction.
The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Software for use in detecting cancer, infectious disease; software for use in prenatal screenings; software for use in operating quantitative PCR instruments; PCR instruments for use in scientific research; scientific research instruments for use in measuring and quantifying copy number of DNA; medical software; computer software; mobile apps; software development tools; downloadable information, publications and educational materials; computer databases; downloadable electronic publications; podcasts and videocasts; data, documents, information, video, sound, text and other media or multi-media, all being electronically recorded or downloadable from the internet or other communications networks; research, laboratory, measuring and monitoring apparatus and instruments. Medical devices for detection of cancer; medical devices for detection of infectious disease; medical devices for use in pre-natal screenings; PCR instruments for use in detecting, diagnosing and treating cancer and infectious disease; PCR instruments for use in pre-natal screenings; instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes; medical, surgical, medical diagnostic and medical screening devices, apparatus and instruments; medical measuring apparatus; medical test kits; medical monitors; apparatus, instruments and test kits for diagnostic testing, self-testing and self-monitoring; parts and fittings for the aforesaid goods. Providing temporary use of non-downloadable computer software for use in detecting cancer, infectious disease; providing temporary use of non-downloadable computer software for use in prenatal screenings; providing temporary use of non-downloadable computer software for use in operating quantitative PCR instruments; providing temporary use of non-downloadable software; hosting websites; design and development of software; technology consultation; installation, updating and maintenance of computer software and programs; computer systems analysis; technical support services relating to computer hardware, software and programs; search engine services; processing and extracting searchable data and information; creating searchable information centres and databases; design, development, implementation, configuration, adaptation and/or customisation of computer software, computer software platforms and internet websites; information and advisory services relating to the foregoing.
45.
Microfluidic siphoning array for nucleic acid quantification
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Software for use in detecting cancer and infectious disease; software for use in prenatal screenings, namely, software for capturing optical images and performing image analysis; software for use in operating quantitative polymerase chain reaction (PCR) instruments; polymerase chain reaction (PCR) instruments for use in scientific research, namely, laboratory apparatus and instruments for amplifying DNA using polymerase chain reaction (PCR); scientific research instruments for use in measuring and quantifying copy number of DNA Medical devices for detection of cancer; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (PCR) platform
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Polymerase chain reaction (PCR) instruments for use in scientific research, namely, laboratory apparatus and instruments for amplifying DNA using polymerase chain reaction (PCR); scientific research instruments for use in measuring and quantifying copy number of DNA Medical devices for detection of cancer; medical devices for detection of infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in detecting, diagnosing and treating cancer and infectious disease, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, polymerase chain reaction (PCR) instruments for use in pre-natal screenings, namely, digital polymerase chain reaction (PCR) platform; medical devices, namely, instruments for use in measuring and quantifying copy number of DNA for medical diagnostic purposes, namely, digital polymerase chain reaction (PCR) platform
48.
METHODS AND SYSTEMS FOR NUCLEIC ACID ANALYSIS AND QUANTIFICATION
The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
49.
METHODS AND SYSTEMS FOR NUCLEIC ACID ANALYSIS AND QUANTIFICATION
The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
50.
Microfluidic siphoning array for nucleic acid quantification
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
B01J 19/02 - Appareils caractérisés par le fait qu'ils sont construits avec des matériaux choisis pour leurs propriétés de résistance aux agents chimiques
B01J 19/24 - Réacteurs fixes sans élément interne mobile
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
B01J 19/24 - Réacteurs fixes sans élément interne mobile
B01J 19/02 - Appareils caractérisés par le fait qu'ils sont construits avec des matériaux choisis pour leurs propriétés de résistance aux agents chimiques
A microfluidic device can have a plurality of microchambers connected to a microchannel via siphon apertures. The microfluidic device may be formed from a thermoplastic and capped by a thermoplastic thin film. The microfluidic device may be used for digital polymerase chain reactions by forcing reagent into the microchambers at low pressure via an inlet, forcing any gas trapped in the microfluidic device to outgas through the thin film by applying high pressure to the microfluidic device via inlets and outlets of the microfluidic device, and applying air at low pressure to the inlets in order to digitize the chambers such that the reagent in each chamber is isolated from the reagent in other chambers by an air gap. After digitization, the device may be used to run a digital polymerase chain reaction process.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Laboratory reagents for scientific and research use, namely,
enzymes and enzymes mixes, nucleotide sets and nucleotide
mixes, reference dye, reaction buffers, detection label,
sold individually or as a kit, for quantitative polymerase
chain reaction and quantitative reverse transcription
polymerase chain reaction.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
(1) Laboratory reagents for scientific and research use, namely, enzymes and enzymes mixes, nucleotide sets and nucleotide mixes, reference dye, reaction buffers, detection label, sold as a kit, for quantitative polymerase chain reaction.
