Abrégé

Described herein are methods for preserving biomaterials by vitrification while reducing or preventing the loss of viability associated with conventional preservation methods. Also described herein are cassettes and methods for using these cassettes for cryopreserving biomaterials (e.g., a bioengineered construct or natural tissue sample).

Classes IPC  ?

• A01N 1/02 - Conservation de parties vivantes

Abrégé

Described herein are cassettes and methods for using these cassettes for cryopreserving biomaterials (e.g., a bioengineered construct or natural tissue sample) by vitrification while reducing or preventing the loss of viability associated with conventional preservation methods.

Classes IPC  ?

• C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse

Abrégé

Cellular material containing living cells is preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing an effective amount of trehalose (in the absence of DMSO and/or any other added cryoprotectants) during a cryopreservation protocol. That is, the cryopreservation protocol is free of cryoprotectant other than trehalose, and the cryopreservation protocol includes: exposing the cellular material to a cryoprotectant formulation containing an effective amount of the trehalose to act as a cryoprotectant, cooling the cellular material at a cooling rate in the range of from ‑3°C/minute to -50°C/minute to a predetermined temperature below -20°C, and obtaining a cryopreserved cellular material that has been warmed.

Classes IPC  ?

• A01N 1/02 - Conservation de parties vivantes

Abrégé

A method for preserving and reducing the immunogenicity of a tissue, the method including obtaining a first tissue, the first tissue being a wild type tissue or genetically modified tissue; forming a second tissue by immersing the first tissue in a first solution having a cryoprotectant concentration of at least about 75% by weight for at least one hour to kill and lyse the cells of the first tissue; forming a third tissue by removing residual cell materials of the second tissue, the residual cell materials of the second tissue being removed by subjecting the second tissue to decellularization in a bioreactor; and subjecting the third tissue to ice-free cryopreservation.

Classes IPC  ?

• A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels

Abrégé

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing at least one mNP and then subjecting the cellular material to a vitrification preservation protocol including nanowarming. This preservation method is particularly effective for cartilage tissues and large blood vessels, and includes, for example, exposing the cellular material to a high concentration cryoprotectant formulation containing at least 0.5 mg/mL Fe mNPs, subjecting the cellular material to a preservation protocol in which ice-induced damage to the cellular material does not occur, and obtaining a cryopreserved cellular material that has been nanowarmed; wherein metabolic activity of the nanowarmed tissue is fully recovered to control values within two days of being rewarmed.

Classes IPC  ?

• A01N 1/02 - Conservation de parties vivantes
• A01N 59/16 - Métaux lourdsLeurs composés

Abrégé

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing at least one sugar and then subjecting the cellular material to a vitrification preservation protocol.

Classes IPC  ?

• A01N 1/02 - Conservation de parties vivantes

Abrégé

The application relates to a method for preserving living cellular material, comprising: exposing a cellular material to medium containing at least one glycolipid and a cryoprotectant for a predetermined amount of time; and after the exposure, subjecting the cellular material to a preservation protocol comprising cryopreservation at a cryopreservation temperature of about -80°C or less.

Classes IPC  ?

• A01N 1/02 - Conservation de parties vivantes