In one aspect, a method of operating an analytical device for measuring at least one analyte within a sample is disclosed, which includes utilizing a digital data processor to receive a sample acquisition rate for introduction of the sample into the analytical device, and to compute one or more optimal operating parameters of the analytical device based on the sample acquisition rate by optimizing a figure-of-merit associated with measurement of the analyte, where the operating parameters include a temporal duration of a measurement cycle and/or selectivity associated with the measurement. The analytical device can be operated at the optimal operating parameters while receiving the sample at the sample acquisition rate to generate sample measurement data corresponding to the analyte. The sample measurement data can be processed to derive information about the analyte.
Methods and systems for controlling a filament of an electron emitter associated with an ion reaction cell in accordance with various aspects of the present teachings may account for inter-filament and inter-instrument variability and can provide improved reproducibility in EAD experiments and ease of use. In some aspects, a method of operating an ion reaction device of a mass spectrometer system is provided. The method comprises applying a calibration drive voltage to a filament of an electron emitter associated with an ion reaction cell and determining a value representative of the calibration electron emission current generated by the filament while having the calibration drive voltage applied thereto. A calibration saturation voltage can be determined by iteratively increasing the calibration drive voltage applied to the filament and determining the value of the calibration electron emission current at each corresponding calibration drive voltage until the filament reaches a saturation condition.
Methods and systems for analyzing a sample that includes a protein and a binding ligand, the method including receiving the sample at an ionization device via non-contact sampling, the first sample being in a non-denaturing carrier solvent, ionizing the first sample, generating a deconvoluted mass spectrum for the ionized first sample, and detecting binding between the protein and the binding ligand in the first sample based at least in part on the deconvoluted mass spectrum. The non-contact sampling is performed via a non-contact sample ejector. Methods and systems for analyzing a sample that includes a protein includes receiving the sample via non-contact sampling, the sample being in one of a plurality of non-denaturing carrier solvents, and for non-denaturing carrier solvent, ionizing the sample, generating a deconvoluted mass spectrum for the ionized sample, and detecting protein binding in the first sample based at least in part on the deconvoluted mass spectrum.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
In one aspect, a heat transfer structure for use in a mass spectrometer can include a collar for surrounding a plurality of rods arranged in a multipole configuration, at least one ceramic pad positioned to be in thermal contact with at least one of the rods, and at least one thermally conductive plate positioned to provide a heat transfer path between the at least one ceramic pad and the collar. The heat transfer structure can further include a bracket mounted on a bracket mounting portion of the collar for transferring heat from the collar to a rail.
Systems, apparatus, and computer-readable storage media are disclosed for analyzing samples of a well plate. Systems may include a well plate, a mass spectrometer, and a computing device. The well plate may include rows of wells. The mass spectrometer may sequentially capture a sample from each well of the rows of wells and generate spectral data that includes mass spectrum data for each captured sample. The computing device may receive the spectral data generated by the mass spectrometer, detect rows of spectral data in the spectral data, wherein each row of spectral data corresponds to a row of wells in the well plate; and generate a spectral data matrix from the detected rows of spectral data such that each row of wells comprises a corresponding row of spectral data in the spectral data matrix.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
6.
SYSTEMS AND METHODS FOR INTRODUCING SOLVENTS TO A SAMPLING INTERFACE
A solvent delivery system for an open port interface (OPI) includes a first diverter which includes a wash solvent inlet, a carrier solvent inlet, and a first diverter outlet. A wash solvent pump is fluidically coupled to the wash solvent inlet. A carrier solvent pump is fluidically coupled to the carrier solvent inlet. A second diverter includes a second diverter inlet fluidically coupled to the first diverter outlet. An OPI port is configured to be coupled to an OPI. A waste outlet is configured to be coupled to a waste container.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
7.
METHODS AND SYSTEMS FOR GENERATING A CONTROLLABLE AXIAL PSEUDOPOTENTIAL BARRIER IN MULTIPOLE ROD SETS OF MASS SPECTROMETERS
The present teachings are generally related to generating a pseudo potential barrier via an axial RF field. As discussed herein in more detail, such a pseudo potential barrier can be employed in a variety of different applications. By way of example, such a pseudo potential barrier can be employed for trapping ions within a rod set comprising a plurality of rods arranged in a multipole configuration. In other applications, an adjustment of the height of the pseudo potential barrier established in a rod set together with application of a DC potential between the rod set and an external electrode can be utilized to cause mass selective extraction of ions trapped within the rod set. In yet other applications, such a pseudo potential barrier can be employed in multiplexing approaches for performing mass spectrometry.
The present teachings are generally related to a method of performing mass spectrometry, which includes dissociating a plurality of precursor ions to generate a first set of product ions, wherein ions with approximately same m/z are associated in a group, and wherein said first set of product ions contains at least two groups of ions with distinct m/z's. The method further includes introducing the first set of product ions into an ion trap; transferring different groups of the first set of product ions during different time intervals from the ion trap to an ion dissociation device so as to cause dissociation of at least a portion of the first set of product ions to generate a second set of product ions, and acquiring a mass spectrum of the second set of product ions.
A method of performing mass spectrometry is disclosed. which comprises introducing a plurality of ions into an ion guide of a mass spectrometer via an inlet orifice thereof. where the ion guide includes a plurality of rods arranged in a multipole configuration and spaced from one another to provide a passageway for transit of the ions therethrough. applying RF voltages to the rods so as to generate an electromagnetic field within the passageway for providing radial confinement of the ions passing through the passageway, identifying a space charge effect, which can adversely affect operation of the mass spectrometer, based on detection of a variation of an intensity of an ion detection signal associated with at least one ion population transmitted through said ion guide and having an m/z ratio greater than a threshold, and in response to said identification of the adverse space charge effect. adjusting at least one of frequency and amplitude of the RF voltages to counteract said space charge effect.
Leak detection systems and methods in accordance with various aspects of the present teachings can, in various embodiments, sequester fluid leaking from the interface between a sample source and the inlet of the ion source, alert an operator as to a leak condition, and/or automatically terminate the experiment. In various aspects, a liquid leak detection system is accordance with the present teachings comprises a collection basin configured to couple to a proximal end of a conduit in fluid communication with a discharge end of an ion source of a mass spectrometer such that the proximal end of the conduit extends through the internal volume of the basin. The system may also comprise a drainage tube having an inlet end opening into an internal volume of the collection basin and configured to drain liquid therefrom, a sensor disposed within the basin or the drainage tube and configured to generate a signal indicative of liquid therewithin, and a processor that is configured to cause a user to be alerted and/or cause an experiment to be terminated upon receiving from the sensor a signal indicative of a leak.
G01M 3/20 - Examen de l'étanchéité des structures ou ouvrages vis-à-vis d'un fluide par utilisation d'un fluide ou en faisant le vide par détection de la présence du fluide à l'emplacement de la fuite en utilisant des révélateurs particuliers, p. ex. teinture, produits fluorescents, produits radioactifs
11.
SYSTEMS AND METHODS FOR ERROR CORRECTION IN FAST SAMPLE READERS
A method and system for detecting a signal measurement error, the method including providing a well plate including error correction wells and sample wells, each sample well including a single sample, and each error correction well including a mixture of samples from two or more sample wells. The method includes receiving an aliquot from the wells at a sample receiver, measuring a signal for the received aliquot, calculating an expected signal for each of the error correction wells, comparing the measured signal to the calculated expected signal for each error correction well, and determining whether an error exists in the signal of at least one sample well. When the error exists, the method correlates the error to one or more sample wells.
Mass spectrometry systems and methods are disclosed. In some embodiments, the mass spectrometry system comprises an ion trap configured to receive a plurality of precursor ions and to load the plurality of precursor ions in a trap region; an electron gun configure to perform an irradiation operation by applying an electron beam to the trap region to produce fragmented ions; an RF gate configured to perform an extraction operation by extracting, from the trap region, a high m/z subset of the fragmented ions with m/z ratios greater than a specific value; and a mass spectrometer configured to perform mass spectrometry analysis on a subset of the fragmented ions remaining in the trap region, wherein an irradiation-extraction operation, including performing the irradiation operation and the extraction operation, is repeated at least twice before performing the mass spectrometry analysis on the subset of the fragmented ions remaining in the trap region.
The present teachings are generally related to generating a pseudo potential barrier via an axial RF field. A first RF voltage is applied to a first pair of rods arranged in a multipole configuration, a second RF voltage is applied to a second pair of rods, and a difference between amplitudes and/or phases of said first RF voltage and said second RF voltage is adjusted. As discussed herein in more detail, such a pseudo potential barrier can be employed in a variety of different applications. By way of example, such a pseudo potential barrier can be employed for trapping ions within a rod set comprising a plurality of rods arranged in a multipole configuration. In other applications, an adjustment of the height of the pseudo potential barrier established in a rod set together with application of a DC potential between the rod set and an external electrode can be utilized to cause mass selective extraction of ions trapped within the rod set. In yet other applications, such a pseudo potential barrier can be employed in multiplexing approaches for performing mass spectrometry.
The presently claimed and described technology provides methods for analyzing an encapsulated biomolecule by loading the encapsulated biomolecule on a capillary electrophoresis (CE) capillary, wherein the CE capillary is filled with a buffer comprising a polymer matrix; applying a voltage to the CE capillary to release the biomolecule from the encapsulating material; and detecting the biomolecule released from the encapsulating material. Kits for analyzing an encapsulated biomolecule are also disclosed.
The presently claimed and described technology is directed to electrophoretic methods for conditioning a capillary using a conditioning buffer including a surface active polymer and a separation reagent including a separation polymer.
Methods and systems for operating an OPI of a sample analysis system, the OPI having a transport liquid conduit and a sample removal conduit and being configured to flow a transport liquid therethrough to a capture region, the method including introducing the OPI at a liquid sample in a sample reservoir while supplying the transport liquid at a first flow rate to the capture region, aspirating a first amount of the liquid sample through the removal conduit via the transport liquid flowing at the first flow rate, switching a flow rate of the transport liquid flowing through the OPI to a second flow rate when a first condition is met, the second flow rate being different from the first flow rate, and aspirating a second amount of the liquid sample through the removal conduit via the transport liquid flowing at the second flow rate. Fast flow rate switching is enabled by a transport liquid flow control apparatus comprising a selectable valve having a pair of valve outlets coupled to different flow paths having different flow resistances.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
17.
REDUCTION OF UNUSED PRECURSORS IN ELECTRON ACTIVATED DISSOCIATION (EAD)
In one aspect, a method of performing mass spectrometry is disclosed, which comprises introducing a plurality of precursor ions into an ion dissociation device, dissociating at least a portion of the precursor ions into a first plurality of product ions having m/z ratios less than an m/z ratio of the precursor ions, discarding at least a portion of any precursor ions remaining subsequent to said dissociation step within said ion dissociation device, and subsequently, allowing at least a portion of said first plurality of product ions to exit from the ion dissociation device through an outlet of the ion dissociation device.
Methods and non-transitory computer readable storage media for loading a reactive agent onto a fluidic device, loading a sample comprising the at least one analyte onto the fluidic device; applying a voltage to the fluidic device, wherein when the voltage is applied the reactive agent remains confined to a region in the separation channel and the at least one analyte is separated from the sample and migrates towards the fluid outlet; generating a first data set and a second data set; converting the second data set by removing a baseline; and correlating the first data set and the converted second data set.
In one aspect, a voltage regulator is disclosed, which comprises a first voltage regulator unit configured for regulating a voltage generated by a positive high voltage source, a second voltage regulator unit configured for regulating a voltage generated by a negative high voltage source, a polarity switch for connecting said first and second voltage regulator units to said positive and negative high voltage sources, respectively, and an output voltage port for receiving a regulated positive and negative high voltage from said first and said second voltage regulator units, respectively.
G05F 1/595 - Dispositifs à semi-conducteurs connectés en série
G05F 1/575 - Régulation de la tension ou de l'intensité là où la variable effectivement régulée par le dispositif de réglage final est du type continu utilisant des dispositifs à semi-conducteurs en série avec la charge comme dispositifs de réglage final caractérisé par le circuit de rétroaction
20.
ELECTROSPRAY EMITTER MODULE FOR CAPILLARY ELECTROPHORESIS DEVICE
A capillary emitter includes a coupling sleeve, a separation capillary inside the coupling sleeve, the separation capillary extending along a longitudinal axis of the coupling sleeve, a tip at an end of the coupling sleeve, the tip comprising an orifice and having an internal base, a first hollow internal cavity defined on one side thereof by the internal base in the coupling sleeve, the first hollow internal cavity comprising the separation capillary, and a fluid pathway within the first hollow internal cavity, the fluid pathway surrounding the separation capillary, wherein, at the internal base of the tip, the separation capillary and the fluid pathway are fluidly connected within the first hollow internal cavity.
An integrated sample processing system including an analyzer and a mass spectrometer is disclosed. The integrated sample processing system can perform multiple different types of detection, thereby providing improved flexibility and better accuracy in processing samples. The detection systems in the sample processing system may include an optical detection system and a mass spectrometer.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 27/624 - Spectrométrie de mobilité ionique différentielle [DMS]Spectrométrie de mobilité ionique à haut champ asymétrique [FAIMS]
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
The present disclosure provides methods and systems for performing mass spectrometry in which at least two batches of precursor ions generated via ionization of at least two different portions of a sample are exposed to electron beams at different energies to cause fragmentation of at least a portion of the precursor ions. In some embodiments, the electron energies can be selected such at one of the electron energies, EIEIO fragmentation can occur while at the other electron energy, EIEO fragmentation channel is not available. The mass spectra corresponding to the two energies can then be utilized to generate a resultant mass spectrum in which mass peaks corresponding to ion fragments generated by EIEIO dissociation are more readily identifiable.
Methods and systems for mass spectrometry are disclosed. In one example, a method comprises: receiving, by a mass spectrometer via a sampling system operably connected thereto, at least one sample containing at least one known compound; modulat-ing at least one instrument parameter of the mass spectrometer through a plurality of instrument parameter values; analyzing the at least one sample while applying each of the plurality of instrument parameter values; acquiring a plurality of mass spectral (MS) datasets each corresponding to one of the applied plurality of instrument parameter values; encoding each of the plurality of MS datasets to generate a corresponding plurality of MS results each corresponding to one of the applied instrument parameter values; and compiling and storing the MS datasets and MS results in a spectral library in association with the applied instrument parameter values.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
Improvements in acoustically dispensed samples that are injected into an open port probe (OPP) are described. Apparatus and method are described that calibrate the volume dispensing determination and mechanisms in the acoustic dispenser to produce accurate and precise volumetric delivery.
B05B 12/00 - Aménagements de commande de la distributionAménagements de réglage de l’aire de pulvérisation
B05B 12/08 - Aménagements de commande de la distributionAménagements de réglage de l’aire de pulvérisation sensibles à l'état du liquide ou d'un autre matériau fluide expulsé, du milieu ambiant ou de la cible
B05B 17/06 - Appareils de pulvérisation ou d'atomisation de liquides ou d'autres matériaux fluides, non couverts par les autres groupes de la présente sous-classe opérant suivant des procédés particuliers utilisant des vibrations ultrasonores
G01F 22/00 - Procédés ou appareils pour la mesure du volume des fluides ou des matériaux solides fluents, non prévus ailleurs
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
25.
Method for Noise Reduction and Ion Rate Estimation Using an Analog Detection System
Ion intensities measured by an ADC detector subsystem are filtered using equivalent TDC event realizations. In one embodiment, an intensity measurement is received for at least one ion made by an ADC detector subsystem for each of m extractions of an ion beam, producing m intensities for the ion. Equivalent TDC event realizations are received for the ion for each intensity of the m intensities, producing m equivalent TDC event realizations. A filtered intensity for the ion is calculated that is a combination of the m intensities and the m event realizations. In another embodiment, for the ion, an equivalent TDC event realization is accumulated for ion events up to a threshold count of event realizations, N, and the ADC intensities are accumulated for all remaining ion events. A filtered intensity for the ion is calculated that is a combination of the equivalent TDC event realization and the ADC intensities.
Various embodiments relate to the reduction of chemical noise at or near the mass of an analyte of interest prior to selection by a mass filter. The use of a narrow bandpass in the ion optics removes chemical noise that can repopulate ions at or near the mass of an analyte of interest by removing other chemical noise ions that can fragment into the same or similar mass as the mass of an analyte of interest through collision induced dissociation.
The disclosed technology provides capillary electrophoresis methods and kits for the separation of multiple components in one or two runs with high resolution.
A method for mass spectrometry comprises ionizing an analyte in an ionizer to generate a plurality of precursor ions; applying temporally modulated energy to the plurality of precursor ions within the ionizer to generate a plurality of fragments of the plurality of precursor ions; and introducing a subset of ions generated in the ionizer into a downstream chamber of a mass spectrometer. In some embodiments, applying the energy may include flowing a gas toward the precursor ions. In various embodiments, the gas may include a heating gas or a cooling gas.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
29.
METHODS AND COMPOSITIONS FOR ELECTOPHORETIC SEPARATIONS
Disclosed and claims are a method for analyzing at least one analyte, the method including introducing a composition into a separation channel, wherein the composition includes at least one pH gradient compound, an amine buffer, and at least one analyte of interest; applying an electric field across the separation channel to create a pH gradient using the at least one pH gradient compound and separate the composition via isoelectric focusing, generating at least one focused analyte peak; mobilizing the at least one focused analyte peak; and wherein the amine buffer is configured to modify the pH gradient.
Described and claimed herein are systems and methods that provide streamlined coupling of CE (e.g., CE-SDS) separation with MS analysis (e.g., such as CE(SDS)-AEX-MS).
G01N 30/96 - Recherche ou analyse de matériaux par séparation en constituants utilisant l'adsorption, l'absorption ou des phénomènes similaires ou utilisant l'échange d'ions, p. ex. la chromatographie en utilisant l'échange d'ions
The n spectra of a DIA method are compared to a library of product ion spectra to identify an initial i compounds corresponding to l spectra. A reinforcement learning algorithm (RLA) is performed. (a) An agent of the RLA performs an action At that includes searching one or more compound databases for compounds related to the i compounds, producing j related compounds, and applying one or more deep learning prediction algorithms to predict k spectra for the i+j compounds. (b) An environment of the RLA compares the k spectra to the n spectra, producing a state, St, in which i+j compounds produce m matching compounds and a reward, Rt, for the agent if m>i. (c) If the Rt is produced, the i compounds are set to the m compounds and the l spectra are set to the k spectra, and steps (a)-(c) are repeated.
Methods and systems for assessing a quality of mass analysis data generated by a mass analysis device, including collecting mass spectrometry data for a given compound, deriving a measured isotope profile based on the collected mass spectrometry data, determining a predicted isotope profile, determining a first quality score for the mass analysis data, the first quality score being based on a relationship between an intensity of the main peak and intensities of the one or more isotope peaks, determining a second quality score for the mass analysis data, the second quality score being based on a signal-to-noise ratio of the mass analysis data, determining an overall quality score as a combination of the first quality score and the second quality score, and assessing a quality of a compound library based on the determined overall quality score.
The presently claimed and described technology provides a sample processing system comprising at least one sample introduction device, wherein the at least one sample introduction device is configured to receive a sample; a mass analyzer coupled to the sample introduction device; a control system configured to at least control the at least one sample introduction device and/or the mass analyzer, wherein the mass analyzer is configured to perform a first mass analysis on the sample, wherein the first mass analysis is mass screening for an analyte of interest in the sample, and wherein if the analyte of interest is detected in the sample, the mass analyzer is configured to perform a second mass analysis, wherein the second mass analysis is a quantitative analysis, comprising: ionizing the sample; monitoring, by mass spectrometry, at least one product ion transition for the at least one analyte and at least one isotopic ion transition for the at least one analyte; determining intensity and/or abundance of the at least one product ion transition and/or the at least one isotopic ion transition; and quantifying the at least one analyte present in the sample using the intensity and/or abundance of the at least one product ion transition and/or isotopic ion transition.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
A method for annotating product ions of a spectrum is disclosed. A product ion mass spectrum is received. A parameter that indicates the mass spectrum was produced using a radical-induced dissociation method is received. At least one charge state mass-to-charge ratio (m/z) adjustment based on the parameter is performed for at least one product ion peak of the mass spectrum and the adjustment is compared to one or more other product ion peaks of the mass spectrum. The at least one product ion peak is annotated based on the comparison. The charge state adjustment includes the loss of an electron or the loss or gain of a hydrogen atom. The radical-induced dissociation method includes electron-based dissociation (ExD), ultraviolet photodissociation (UVPD), or infrared photodissociation (IRMPD).
The presently described and claimed disclosure relates to method for characterizing nucleic acid purity comprising denaturing a nucleic acid sample, loading the nucleic acid sample onto a capillary electrophoresis (CE) capillary, wherein the CE capillary is filled with a buffer comprising a polymer matrix, applying a separation voltage to the CE capillary, wherein during the separation of the nucleic acids, the temperature of the CE capillary is increased, and detecting nucleic acids separated from the nucleic acid sample with a detector. Kits and instructions for use are also described.
Calibrant compositions comprising a plurality of synthetic peptides for calibration of a mass spectrometer in either positive or negative mode are provided. The synthetic peptide calibrants exhibit desirable ionization characteristics, solubility, and solution stability, and are easily washed out of the system such that no interfering signals are left behind. Methods for calibration of mass spectrometer in positive or negative ion mode using a single calibrant composition are also provided.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
37.
METHODS AND SYSTEMS FOR DETERMINING MOLECULAR MASS
Methods and systems for adjusting instrument setting, improving fidelity of isotope pattern for mass spectra, and/or determining molecular mass with improved accuracy are disclosed. In one example, a method for determining Mmono of a compound of interest in a sample using a mass spectrometer is provided. The method comprises: (1) tuning or adjusting instrument setting of the mass spectrometer using at least one known compound, wherein the instrument setting comprises at least one parameter for improving accuracy; (2) analyzing the compound of interest using the adjusted instrument setting to obtain a mass spectrum thereof, wherein the mass spectrum comprises an isotope pattern thereof; and determining the Mmono of the compound of interest from the mass spectrum thereof.
Systems and methods are disclosed for performing a DDA mass spectrometry experiment. A precursor ion survey scan of a mass range is performed to generate a precursor ion peak list. A series of steps are performed for each precursor ion peak of the peak list. A peak mass range including the precursor ion peak is selected. A precursor ion mass selection window with a width smaller than the peak mass range is canned across the peak mass range in overlapping steps, producing a series of overlapping windows across the peak mass range. Each overlapping precursor ion mass selection window of the series is fragmented. Product ions produced from each overlapping precursor ion mass selection window of the series are mass analyzed, producing a product ion spectrum for each overlapping precursor ion mass selection window of the series and a plurality of product ion spectra for the peak.
A method for determining a convolved peak intensity in a sample trace includes ejecting a plurality of sample ejections from a sample well plate. An ejection time log is generated which includes an ejection time of each of the plurality of sample ejections from the sample well plate. The plurality of sample ejections is analyzed with a mass analyzer. The sample trace of intensity versus time values is produced for the plurality of sample ejections based on the analysis. A known peak shape is obtained. A convolved peak intensity is determined for a convolved peak of the sample trace based at least in part on the known peak shape and the ejection time log.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
Methods and system that determines disulfide and trisulfide linkages within analytes (e.g., polypeptides) is described. In certain aspects. a sample comprising polypeptides (such as an antibody) may be subjected to dissociation using an electron activated dissociation (which can include electron capture dissociation and electron transfer dissociation) and the fragmentated portions are analyzed using a mass spectrometer to produce a spectrum. The spectrum is analyzed by a processor to identify peaks from the spectrum that are related to one another in the spectra by a separation of 32 mass units. In identifying an antibody comprising peptide segments linked via a trisulfide bond. for example, four different peaks representing two different peptides are searched for and identified representing a first peptide portion having mass/charge of A and A+32 and a second peptide having mass/charge of B and B+32.
G16C 20/20 - Identification d’entités moléculaires, de leurs parties ou de compositions chimiques
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G16B 40/10 - Traitement du signal, p. ex. de spectrométrie de masse ou de réaction en chaîne par polymérase
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/42 - Spectromètres à stabilité de trajectoire, p. ex. monopôles, quadripôles, multipôles, farvitrons
41.
METHODS AND SYSTEMS FOR PROCESSING IN REAL-TIME AND USING GAUSSIAN
Systems and methods are provided for processing in real-time and using Gaussian fitting digitized signals from ions detection in time-of-flight (TOP) mass spectrometry. Acquisition/analog-to-digital conversion may be applied in the course of Ion detection during time-of-flight (TOP) mass spectrometry, with the acquisition/analog-to-digital conversion including generating, in response to detection of ions, one or more time-of-flight (TOP) based signals, and digitizing, using analog-to-digital conversion, the one or more TOP based signals, to generate corresponding digitized data. The digitized data may then be processed, in real-time and based on use of Gaussian fitting, to generate result data corresponding to the time-of-flight (TOP) mass spectrometry. The Gaussian fitting may comprise applying second (2nd) degree polynomial fit, such as by least squares via QR factorization.
Methods and systems for background processing include ionizing a first sample and a second sample, the first sample including the target molecule, and the second sample including a target molecule-ligand combination, capturing a raw mass spectrum for the ionized first sample and second sample, generating a deconvoluted mass spectrum for the first sample and the second sample, determining an intensity ratio of a background intensity at a mass window corresponding to a sum of a mass of the target molecule and a mass of the ligand in the first sample to a signal intensity at a mass window corresponding to the target molecule in the first sample, and determining, based on the determined intensity ratio, a background intensity of the target molecule- ligand combination in the deconvoluted mass spectrum of the second sample at a mass window corresponding to the mass of the target molecule-ligand combination.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
C12Q 1/6872 - Méthodes de séquençage faisant intervenir la spectrométrie de masse
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G16C 20/20 - Identification d’entités moléculaires, de leurs parties ou de compositions chimiques
43.
SYSTEMS AND METHODS FOR FLASH BOILING OF A LIQUID SAMPLE
A method of ejecting a sample from a nebulizer nozzle fluidically coupled to a port via a transfer conduit includes receiving at the port a transport liquid and the sample. The transport liquid and the sample in the transfer conduit is transported from the port to a transfer conduit exit comprising an electrode tip. The transport liquid is ejected from the transfer conduit exit. The sample is ejected from the transfer conduit exit substantially simultaneously with ejecting the transport liquid. During ejection of the transport liquid and the sample from the transfer conduit exit, a pressure is generated at the transfer conduit exit substantially similar to a vapor pressure of the transport liquid.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
44.
Systems and Methods for Background Ion Detection in Mass Spectrometry
A method for performing mass spectrometry (MS) comprises receiving MS data corresponding to a plurality of MS runs, wherein MS data corresponding to an MS run of the plurality of MS runs comprises detected intensities for a plurality of mass over charge ratios (MZ values) during the MS run; finding a recurrent MZ value of the plurality of MZ values, wherein a detected intensity for the recurrent MZ value appears as a recurrent peak in MS data corresponding to a subset of the plurality of MS runs; and the subset of the plurality of MS runs includes at least two MS runs of the plurality of MS runs; and identifying the recurrent MZ value as corresponding to a background ion.
In one aspect, a mass spectrometer is disclosed, which includes an ion path along which an ion beam can propagate, and an ion beam deflector positioned in the ion path and configured to modulate transfer of an ion beam received from an upstream section of the ion path to a downstream section thereof, said ion beam deflector comprising at least one electrically conductive electrode positioned relative to one another to provide an opening through which the ion beam can pass, where the two electrodes are electrically insulated relative to one another so as to allow maintaining each electrode at a DC potential independent of a DC potential at which the other electrode is maintained.
A method of sampling with an open port interface (OPI) comprises operating the OPI. A liquid sample is contacted with the OPI. An aliquot from the liquid sample is pinned to the OPI. The aliquot includes a predetermined volume which terminates contact between the OPI and the liquid sample. The aliquot is aspirated from the OPI via a removal conduit within the OPI.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
47.
OPTIMIZATION OF DMS SEPARATIONS USING ACOUSTIC EJECTION MASS SPECTROMETRY (AEMS)
Disclosed are methods and systems that provide for the analysis of one or more analytes of interest in an acoustic ejection mass spectrometer (AEMS) system that incorporates an open port interface (OPI) and differentiation mass spectrometry (DMS) that allows for operation of the system in a pseudo-continuous mode to scan and determine optimal DMS settings for the one or more analytes of interest, and for operation of the system in a discontinuous mode to analyze for the presence of the one or more analytes of interest in a sample.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
G01N 27/624 - Spectrométrie de mobilité ionique différentielle [DMS]Spectrométrie de mobilité ionique à haut champ asymétrique [FAIMS]
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
Methods and systems for spectral comparison and quality assessment are disclosed. In one example, a method for assessing quality of a mass spectrum (MS) of a sample is provided. The method comprises: predefining one or more features or attributes indicative of the sample quality with reference to a target compound; and calculating a quality score for the MS with respect to the selected features or attributes.
Methods and systems for identifying analytes in a sample using mass spectrometry are provided. A method for identifying analytes in mass spectrometry data comprises: introducing a sample to a mass spectrometer; analyzing the sample with the mass spectrometer in a plurality of cycles; generating, for each cycle, a mass spectrum comprising at least one peak; annotating peaks in the mass spectrum based on their relationships; assigning best ion types to each peak; processing each cycle of the mass spectrum to assign a score to each of the at least one peak thereof with respect to the likely neutral mass related to the peak; grouping peaks that share a common neutral mass; and outputting the analyte neutral mass.
Methods and systems for building and using an analyte library are provided. One aspect is a method for building an analyte library, the method comprising receiving mass spectrum data from analysis of a sample using mass spectrometry, mass spectrum data including a mass spectrum and a sample matrix, and the sample including an analyte, identifying peaks in the mass spectrum, assigning at least one ion type to the peaks, annotating the peaks for the analyte based on the sample matrix, extracting an ion fingerprint for the analyte based on the annotated peaks and storing an analyte identification entry including the ion fingerprint for the analyte.
In one aspect, a mass spectrometer is disclosed, which includes an ion trapping device, at least one pressurized ion guide configured to allow establishment of an adjustable axial field therein such that the axial field can be adjusted to operate the pressurized ion guide in any of a fast and a slow operational setting, a mass analyzer for receiving ions passing through the pressurized ion guide and configured to provide mass analysis of the received ions, and a controller in communication with said at least one pressurized ion guide for causing the adjustment of the axial field so as to switch the operational setting of the pressurized ion guide between the fast and the slow operational settings based on an operational mode of the mass spectrometer.
A method of calibrating a signal measurement system includes performing a first number of calibration measurements for a first number of samples of a compound, the samples having known concentrations, by measuring a signal for each sample, receiving a signal for a sample having an unknown concentration of the compound; selecting a second number of calibration measurements from the first number of calibration measurements, the second number being smaller than the first number; performing a regression on the selected second number of calibration measurements; and determining the unknown concentration based on the performed regression.
An ion mass filter for use in a mass spectrometer is disclosed, which includes a plurality of rods arranged in a multipole configuration to provide a passageway through which ions can travel, said plurality of rods being configured for application of RF voltages thereto to generate an electromagnetic field within the passageway for providing radial confinement of the ions and further configured for application of a DC voltage thereto, and at least two pairs of auxiliary electrodes interspersed between the plurality of multipole rods, where one pair forms a first pole of the auxiliary electrodes and the other pair forms a second pole of the auxiliary electrodes. A controller can provide one or more control signals to the DC voltage source so as to switch the polarity of the DC voltage differential between the two poles according to a predefined criterion.
A method for improved mass spectrometry by determining charge state of precursor ions from an analysis of product ions, includes receiving sample ions. A group of precursor ions is selected from the received sample ions based on mobility. A fragmentation device fragments the group of precursor ions to produce a group of product ions. A tandem mass spectrometry analysis is performed on the group of product ions to generate an intensity and mass-to-charge ratio (m/z) of the group of product ions. An ionogram is generated, based on the generated intensities and mass, to charge ratios for the groups of product ions generated for each of the mobility selection. The ionogram includes a first axis representing compensation voltage value and another axis representing intensity. A product ion peak is identified in the ionogram. At least one peak characteristic is identified of the product ion peak. A charge state of a precursor ion that was fragmented to form the product ions represented in the product ion peak is determined based on the at least one peak characteristic of the produce ion peak.
In one aspect, a method of operating a mass spectrometer is disclosed, which comprises ionizing a sample to generate a plurality of ions, and introducing at least a portion of the ions into an inlet orifice of the mass spectrometer. At least a portion of the ions and/or fragments thereof is detected by a downstream detector to generate a plurality of ion detection events, and the ion detection events are monitored to determine an ion count. The ion count is compared with a reference level to determine whether the detected level exceeds the reference level.
Systems and methods are disclosed for analyzing a sample using overlapping precursor isolation windows. A mass analyzer of a tandem mass spectrometer is instructed to select and fragment at least two overlapping precursor isolation windows across a precursor ion mass range of a sample using a processor. The tandem mass spectrometer includes a mass analyzer that allows overlapping precursor isolation windows across the mass range of the sample.
In one aspect, an ion filter for use in a mass spectrometer is disclosed, which includes a plurality of rods arranged in a multipole configuration to provide a passageway through which ions can travel, said plurality of rods being configured for application of RF voltages thereto to provide an electromagnetic field within the passageway for providing radial confinement of the ions and further configured for application of a DC voltage thereto. At least two pairs of auxiliary electrodes are interspersed between the plurality of rods and are configured for application of a DC bias voltage with one polarity to one of said pairs and a DC bias voltage with an opposite polarity to the other one of said pairs to provide a DC potential difference between the auxiliary electrodes and the plurality of rods.
One or more known compounds are separated from a mixture using a separation device that allows processor-controlled adjustment of a separation parameter. The separated compounds are ionized and, for each cycle of a plurality of cycles, a mass spectrometer executes on the ion beam a series of MRM transitions read from a list. Two or more contiguous groups of MRM transitions to be monitored separately are received. Each group includes at least one sentinel transition that identifies a next group that is to be monitored and identifies a value for the separation parameter for the next group. A first group is placed on the list. When a sentinel transition of the first group is detected, a next group identified by the sentinel transition is placed on the list and the separation parameter is adjusted to a value identified by the sentinel transition for the next group.
Methods and systems for identifying one or more analytes in a sample are provided. One aspect is a method of predicting an identity of analytes in an unknown sample, the method comprising accessing a database comprising a plurality of results from analyzing samples using mass spectrometry to identify analytes, the plurality of results including annotated ion fingerprints, training a machine learning model with the plurality of results, and applying the machine learning model to the unknown sample to predict an identity of one or more analytes in the unknown sample.
Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which the flow of desorption solvent within a sampling probe fluidly coupled to an ion source can be selectively controlled such that one or more analyte species can be desorbed from a sample substrate inserted within the sampling probe within a decreased volume of desorption solvent for subsequently delivery to the ion source. In various aspects, sensitivity can be increased due to higher desorption efficiency (e.g., due to increased desorption time) and/or decreased dilution of the desorbed analytes. The methods and systems described herein can additionally or alternatively provide for the selective control of the flow rate of the desorption solvent within the sampling interface so as to enable additional processing steps to occur within the sampling probe (e.g., multiple samplings, reactions).
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
G01N 30/00 - Recherche ou analyse de matériaux par séparation en constituants utilisant l'adsorption, l'absorption ou des phénomènes similaires ou utilisant l'échange d'ions, p. ex. la chromatographie
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
61.
INTENSITY-INDEPENDENT PRECURSOR INFERENCE IN MASS SPECTROSCOPY
Methods for correlating a product ion in a mass spectrum to a precursor ion are disclosed herein, comprising determining a precursor ion ml z corresponding to the product ion as an m/z at which the product ion appears in a maximum amount of the series of mass spectra. Methods also can comprise obtaining a series of mass spectra for a sample across a mass range, each of the series of mass spectra having a precursor ion transmission window defined by a width (W) that overlaps with that of at least two of the series of mass spectra by a step size (S).
Disclosed are methods for separating target and non-target analytes in a sample. The methods can utilize an acoustic droplet ejector (ADE) and an open port interface (OPI) to achieve liquid chromatography (LC)-like separation for an analytical instrument such as, for example, a mass spectrometer.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
63.
Automated analysis of complex matrices using mass spectrometer
Improved systems, apparatus, methods, and programming useful for the automated analysis of complex compounds using mass spectrometers. Systems, apparatus, methods, and programming according to the invention provide for the automatic determination by a controller 54 of a mass spectrometer 14, 214 of an analysis operation to be implemented using the mass spectrometer, the analysis operation adapted specifically for analysis of one or more substances based contained within a compound based on identification of the compound and/or substances provided by a user of the spectrometer, and a database 66 or other library of information concerning suitable processes or process steps for analyzing substances.
A method for measuring a concentration of an analyte in a sample includes: sampling, from a first sample, a first one or more droplets for mass analysis, wherein the first sample includes the sample; performing mass analysis on the first one or more droplets to determine a first intensity of an analyte in the first one or more droplets; sampling, from a second sample, a second one or more droplets for mass analysis, wherein the sec-ond sample includes the sample and a first spike of the analyte; performing mass analysis on the second one or more droplets to determine a second intensity of the analyte in the second one or more droplets; fitting a curve to the first intensity and the second intensity; and based on the fitted curve, calculating an analyte concentration for the sample.
Systems and methods are provided for correcting a measured retention time or expected retention time of an ion intensity measurement. A measured sentinel retention time is received for each of a plurality of sentinel ion intensity measurements. During acquisition, sentinel analysis is performed. In sentinel analysis, a plurality of ion intensity measurements is divided into two or more groups so that different groups of the two or more groups are measured separately. At least one sentinel ion intensity measurement in each group of the two or more groups is selected to identify a next group of the two or more groups to be measured. A measured retention time or an expected retention time of at least one non-sentinel ion intensity measurement of the two or more groups is corrected using the plurality of measured sentinel retention times.
Methods and systems for handling and/or analyzing samples are provided. In one example, a method comprises: introducing (406), with a liquid handler (322), at least one assisting agent into a sample well (310) of a well plate (312), wherein the sample comprises a sample volume; and ejecting (408), with an acoustic droplet ejector, ADE, (306), a mixture comprising the sample mixed with the at least one assisting agent from the sample well, wherein the at least one assisting agent interacts (406) with an analyte of the sample to limit gel formation at a top surface of the sample volume, prior to the mixture ejection.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
67.
IDENTIFICATION OF AMINO ACID ISOMERS USING DIAGNOSTIC FRAGMENT IONS IN MS/MS DATA
Computer-implemented methods and non-transitory computer readable storage media for isobaric amino acid differentiation, where an MS/MS data set may be processed, wherein the processing includes determining whether a protein sample comprises at least one isobaric amino acid, analyzing whether any diagnostic fragment ions of the at least one isobaric amino acid are present in the protein sample, assigning a score to the at least one isobaric amino acid based on the diagnostic fragment ions present in the MS/MS data set, evaluating the identity of the at least one isobaric amino acid present in the protein sample based on the assigned score, and reporting the evidence of the identity of the at least one isobaric amino acid present in the protein sample.
A precursor ion transmission window is moved in overlapping steps across a precursor ion mass range. The precursor ions transmitted at each overlapping step by the mass filter are fragmented or transmitted. Intensities or counts are detected for each of the one or more resulting product ions or precursor ions for each overlapping window that form mass spectrum data for each overlapping window. Each unique product ion detected is encoded in real-time during data acquisition. This encoding includes sums of counts or intensities of each unique ion detected the overlapping windows and positions of the windows associated with each sum. The encoding for each unique ion is stored in a memory device rather than the mass spectral data. A deblurring algorithm or numerical method is used to determine a precursor ion of each unique ion from the encoded data.
G06F 17/17 - Évaluation de fonctions par des procédés d'approximation, p. ex. par interpolation ou extrapolation, par lissage ou par le procédé des moindres carrés
A method for identifying peaks in a mass spectrum is provided. The method includes: accessing a mass spectrum (300), having an intensity signal, generated for analysis of a sample; performing a wavelet transformation on the intensity signal to generate a wavelet space representation (310) of the intensity signal; generating a scale-space-processing (SSP) response signal (412, 414, 416) from the wavelet space representation of the intensity signal, wherein the SSP response signal (412, 414, 416) represents the SSP response from the wavelet scale representation (310) at different wavelet scales for a particular m/z starting position (312, 314, 316); identifying a first wavelet scale for a first local maximum in the SSP response signal; based on the first wavelet scale, detect a first baseline intensity signal; subtracting the first baseline intensity signal from the intensity signal to generate a first adjusted intensity signal; and detecting one or more peaks in the first adjusted intensity signal.
A liquid handling system for a mass spectrometer (MS), the liquid handling system including an open port interface (OPI) including a body defining a port and an internal volume. At least one removal conduit is disposed in the body and fluidically coupled to the internal volume. A plurality of transfer conduits is fluidically coupled to the at least one removal conduit. A single one of a plurality of nebulizer nozzles are fluidically coupled to each of the plurality of transfer conduits.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
71.
SYSTEMS AND METHODS FOR PURITY CALCULATION FOR THE COMPOUND QC WORKFLOW
A method for quantifying a purity of a target analyte in a sample includes introducing into a mass spectrometer, via a sample introduction, a plurality of sample ions generated by ionizing at least a portion of analytes in the sample; obtaining, via the mass spectrometer, a plurality of mass spectra including a sample mass spectrum associated with the sample introduction; analyzing the sample mass spectrum to identify a target signal corresponding to the target analyte; deriving, from the target signal, a target signal intensity corresponding to the target analyte; deriving, from at least a portion of the sample mass spectrum, a sample signal intensity; and quantifying the purity of the target analyte based on the target signal intensity and the sample signal intensity.
In one aspect, a method of acquiring mass data in a mass spectrometric system is disclosed, which includes introducing a plurality of ions into a mass filter providing a mass selection window to allow passage of precursor ions having m/z ratios within the mass selection window through the mass filter and scanning the mass selection window and adjusting a width thereof across a mass range during acquisition of mass data.
A method for determining a composition of a sample utilizing mass spectrometry includes introducing a plurality of sample ions, obtaining a plurality of mass spectra including a sample introduction mass spectrum associated with the sample introduction event, identifying a set of background signals, identifying a set of sample ion signals by removing background ions, and deriving, from the set of sample ion signals, an ion list corresponding to the composition of the sample.
In one aspect, a mass spectrometer system includes an ion mobility spectrometer (IMS), a gas supply for providing a curtain gas, a modifier supply for providing a liquid modifier, and a nebulizer for receiving the liquid modifier from the modifier supply and generating liquid droplets for delivery to a curtain chamber of the IMS. The spectrometer includes a fluid manifold for receiving the liquid modifier and delivering the liquid modifier to the nebulizer and to receive the curtain gas from the gas supply and provide a first portion of the curtain gas to the nebulizer as a nebulizing gas and provide a second portion of the curtain gas as a sheath flow to a region in vicinity of a nozzle of the nebulizer such that a combination of the sheath flow and gas exiting the nebulizer flows as a curtain gas entraining the liquid droplets to the curtain chamber.
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
B05B 5/00 - Pulvérisation électrostatiqueDispositifs de pulvérisation comportant des moyens pour charger électriquement le pulvérisatPulvérisation de liquides ou d'autres matériaux fluides par voies électriques
A mass spectrometer that includes a mass filter and a TOF mass analyzer receives the ion beam from an ion source device that ionizes a compound of a sample. The mass filter selects a precursor ion mass range and the mass analyzer mass analyzes the mass range. A continuous flow of selected precursor ions is maintained between the mass filter and the mass analyzer. A first set of parameters is applied to the mass spectrometer to produce a resolution above a first resolution threshold. A space charge effect is detected by determining if the measured TIC exceeds a TIC threshold or the measured resolution is less than the first resolution threshold. If a space charge effect is detected, at least one precursor ion transmission window with a width smaller than the mass range is applied to the ion beam by the mass filter and mass analyzed to reduce the space charge.
Systems and methods are disclosed for timed introduction of samples into a mass spectrometer may include receiving a plurality of sample ion pulses in a mass spectrometer from a sampling interface, where the sample ion pulses are received at a pre-determined time pattern; detecting the received sample ion pulses to generate a signal; isolating an analyte signal by signal conditioning the generated signal based on the pre-determined time pattern; and identifying a presence of an analyte based on the isolated analyte signal. The signal conditioning may include pulse-based averaging based on the pre-determined time pattern or may include converting the generated signal to a frequency-domain signal and calculating a modulus to isolate the analyte signal. The pre-determined time pattern may be periodic where the signal conditioning comprises performing a Fourier Transform on the signal to convert it to a frequency-domain signal.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
77.
POWER SUPPLY INCLUDING AMPLITUDE CALIBRATION AND PHASE CORRECTION FOR MASS SPECTROMETRY
In one aspect, a circuit for generating an asymmetric waveform for application to electrodes of a differential mobility mass (DMS) spectrometer is disclosed, which includes two digital waveform synthesizers for generating digital waveforms, which are converted to analog waveforms for application to electrodes of the DMS spectrometer. Analog feedback signals associated with the applied waveforms are digitized into digital amplitude calibration feedback signals and digital phase correction feedback signals. An amplitude calibration circuit is employed apply an RF calibration factor to at least one of the digital waveform synthesizers based on digital the amplitude calibration feedback signals. A digital passband filter is employed to filter the digital phase correction feedback signals, which are then employed to determine a phase correction signal for application to at least one of the digital waveform synthesizers for maintaining a substantially constant phase difference between the waveforms generated by the digital waveform synthesizers.
In one aspect, a circuit for generating an asymmetric waveform for application to electrodes of a differential mobility mass (DMS) spectrometer is disclosed, which includes two digital waveform synthesizers for generating digital waveforms, which are converted to analog waveforms for application to electrodes of the DMS spectrometer. Analog feedback signals associated with the applied waveforms are digitized and a digital passband filter is employed to filter the digital feedback signals, which are then employed to determine a phase correction signal for application to at least one of the digital waveform synthesizers for maintaining a substantially constant phase difference between the waveforms generated by the digital waveform synthesizers.
Examples of the disclosure relate to a modular radiant light generation apparatus, including a modular receiver including a recessed portion, a radiant light source on a bottom surface of the recessed portion of the modular receiver, a first fitting on the radiant light source, a fiber optic jacket including a fiber optic core therein, a first portion of the fiber optic jacket coupled to the second fitting being inserted in the first fitting, and a third fitting including a threaded projection and a biasing element the second fitting being coupled to the first fitting being configured to align the fiber optic core with the radiant light source, and the biasing element being configured to keep the fiber optic core in contact with the radiant light source.
A method for calibrating a mass spectrometry (MS) system includes: receiving at least one input through a corresponding to a calibrator in a sample, wherein the sample includes an analyte, and wherein the sample is analyzed by the MS system; automatically determining a plurality of transitions in the sample corresponding to the calibrator according to natural abundances of isotopes; automatically determining concentrations of the plurality of transitions according to a concentration of the calibrator in the sample; identifying a plurality of transitions as calibrator transitions and for identifying a different one of the plurality of transitions as an internal standard; detecting a concentration of each of the plurality of transitions and the calibrator in the sample; automatically calibrating the MS system based in part on the detected concentrations of the plurality of calibrator transitions and the internal standard; and detecting the concentration of the analyte in response to execution of the calibration instructions.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
G06F 3/0484 - Techniques d’interaction fondées sur les interfaces utilisateur graphiques [GUI] pour la commande de fonctions ou d’opérations spécifiques, p. ex. sélection ou transformation d’un objet, d’une image ou d’un élément de texte affiché, détermination d’une valeur de paramètre ou sélection d’une plage de valeurs
82.
Bent PCB Ion Guide for Reduction of Contamination and Noise
In one aspect, an ion guide for use in a mass spectrometer is disclosed, which comprises a first plurality of conductive electrodes disposed on a first surface, a second plurality of conductive electrodes disposed on a second surface, wherein the two surfaces are positioned relative to one another and shaped so as to provide a passageway having an inlet for receiving an ion beam and an outlet through which target ions of interest exit the passageway. The ion guide further includes an orifice formed in at least one of those surfaces through which neutral species and/or large ion clusters, when present in the ion beam, exit the ion guide.
In one aspect, a calibration system for use in a mass spectrometer having an open port interface (OPI) for receiving a sample for mass analysis is disclosed, which includes a fluidic junction having a first inlet in fluid communication with a first reservoir, which is configured for storing a calibration liquid, and a second inlet in fluid communication with a second reservoir, which is configured for storing a transport liquid. The fluidic junction can further include an outlet in fluid communication with the first and second inlets such that any of the calibration liquid and the transport liquid can exit the fluidic junction via said outlet.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
84.
RF AMPLITUDE AUTO-CALIBRATION FOR MASS SPECTROMETRY
Systems and methods are disclosed for RF amplitude auto-calibration for mass spectrometry. As non-limiting examples, various aspects of this disclosure provide in a mass spectrometer comprising an RF gain block, a peak detector, and a controller: applying a DC voltage to the coil using the controller; measuring a DC calibration voltage using the peak detector; applying an RF voltage to the RF gain block using the controller; measuring an RF calibration voltage; calculating an RF calibration factor based on the measured calibration voltages using the controller; and during operation, and applying a combined RF and DC signal to the RF gain block based on the RF calibration factor. The DC voltage may be generated utilizing a first signal sent from the controller to the RF gain block via a DC amplifier.
Computer-implemented methods and non-transitory computer readable storage media for determining compound structures from two or more MS/MS data sets obtained using orthogonal fragmentation, where the two or more MS/MS data sets may be processed, aligned, and consolidated to determine a lead candidate structure.
Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings. MS-based systems and methods are provided in which the flow of solvent into an open port sampling probe fluidly coupled to an ion source can be selectively stopped during the addition of one or more reagents into the drained open end of the sampling probe. Upon re-initiating the flow of solvent, the reagents and/or the reaction products can be delivered to the ion source. In one aspect, a method for chemical analysis is provided, the method comprising directing a flow of a first solvent from a solvent conduit to an ion source via a sampling space of a sampling probe, wherein the sampling space is at least partially defined by an open end of the sampling probe. The flow of the first solvent into the sampling space from the solvent conduit may be terminated for a first duration, and the sampling space drained. A second solvent and one or more reactants may then be added to the drained sampling space through the open end during the first duration. Thereafter, the flow of the first solvent may again be directed from the solvent conduit to the ion source via the sampling space such that the second solvent is delivered to the ion source, and such that one or more reaction products contained within the second solvent and generated by said one or more reactants may be ionized for mass spectrometric analysis.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
87.
AUTOMATED SYSTEMS AND METHODS FOR SEPARATING COMPOUNDS
A gas introduction system for a differential mobility spectrometer (DMS) includes a manifold including a gas inlet and a gas outlet. A mixing channel fluidically couples the gas inlet to the gas outlet. A plurality of modifier liquid supply inlets is coupled to the mixing channel and a plurality of selectively operable valves. One of the plurality of selectively operable valves is coupled to one of the plurality of modifier liquid supply inlets. A control system is in communication with each of the plurality of the selectively operable valves. The control system is configured to actuate each of the plurality of selectively operable valves.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
G01N 27/624 - Spectrométrie de mobilité ionique différentielle [DMS]Spectrométrie de mobilité ionique à haut champ asymétrique [FAIMS]
The technology relates to systems and methods for performing mass spectrometry analysis of a sample. An example method may include receiving, as input via an input device, a target mass-to-charge (m/z) ratio for a fragment ion of interest; setting a target m/z range based on the target m/z ratio; ionizing the sample to generate precursor ions; fragmenting the precursor ions to generate fragment ions having a range of mass-to-charge ratios larger than the target m/z range; accelerating the fragment ions to a detector such that fragment ions inside and outside of the target m/z ratio are detected; summing a count of fragment ions within the target m/z range without storing ion counts for fragment ions outside of the target m/z range; and storing the summed ion count as corresponding with the target mass-to-charge ratio.
Systems and methods are disclosed for determining an isomer of a non¬ derivatized glycan. A first intensity of a first product ion and a second intensity of a second product ion for a non-derivatized glycan produced using electron-based dissociation (ExD) mass spectrometry are received. The isomer is determined by differentiating between a first isomer and a second isomer of the non-derivatized glycan. Differentiating between the first isomer and the second isomer includes comparing an intensity ratio of the first intensity and the second intensity to a ratio threshold value. The ratio threshold value differentiating the first isomer from the second isomer varies with the kinetic energy of the mass spectrometry The electron kinetic energy of the ExD is greater than or equal to 11 eV and less than or equal to 25 eV.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
90.
SYSTEMS AND METHODS FOR INTRODUCING SAMPLES TO OPEN PORT INTERFACE
United States of America, as represented by the Secretary, Department of Health and Human Services (USA)
Inventeur(s)
Verma, Meghav
Michael, Samuel
Janiszewski, John
Liu, Chang
Covey, Thomas R.
Abrégé
A method of processing a sample plate containing a plurality of samples includes aspirating simultaneously, from the sample plate, a first sample droplet from a first sample of the plurality of samples with a first pipette and a second sample droplet from a second sample of the plurality of samples with a second pipette. The sample plate also includes dispensing sequentially, from the first pipette and the second pipette, the first sample drop and the second sample drop into an open port interface (OPI).
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
An ion mirror for use in a time-of-flight mass spectrometer includes a mirror ring sub-assembly for creating an electric potential field to decelerate incoming ions and accelerate outgoing ions, and a grid sub-assembly comprising one or more grid plates, which define bounds of the electrical potential field of the ion mirror. The mirror ring sub-assembly and the grid sub-assembly are coaxially superposed, and the mirror ring sub-assembly is mechanically engaged with the grid sub-assembly only at one of the grid plates.
In one aspect, a method of introducing a sample into an open port interface (OPI) of a mass spectrometer is disclosed, which includes mixing the sample with a solvent in which a matrix of an aqueous phase of the sample is immiscible and in which at least a target analyte, when present in the sample, is miscible so as to extract at least a portion of the target analyte into said at least one solvent, thereby generating a multi-phase liquid having said aqueous phase and one or more organic phases, wherein at least one of those organic phases contains at least a portion of the target analyte. In some embodiments, the method further calls for ejecting a plurality of droplets from at least one of the phases of the multi-phase liquid for introduction into the OPI of the mass spectrometer.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
In one aspect, a method of assessing hydrophobicity of a target analyte is disclosed, which includes introducing a sin-gle-phase system containing a concentration of the target analyte into a mass spectrometer to acquire at least one mass signal associated with the target analyte, introducing a phase-separated system (e.g., a two-phase system) containing substantially the same concentration of the target analyte into the mass spectrometer to acquire at least one mass signal associated with said target analyte, and utilizing a ratio of the intensities of the mass signals to assess hydrophobicity of the target analyte.
G01N 23/2208 - Combinaison de plusieurs mesures, l'une au moins étant celle d’une émission secondaire, p. ex. combinaison d’une mesure d’électrons secondaires [ES] et d’électrons rétrodiffusés [ER] toutes les mesures portant sur l’émission secondaire, p. ex. combinaison de la mesure ES et des rayons X caractéristiques
G16C 20/30 - Prévision des propriétés des composés, des compositions ou des mélanges chimiques
94.
Methods and Systems for Injecting Ions into an Electrostatic Linear Ion Trap
Systems and methods described herein provide for the injection of ions into an ELIT at a variety of kinetic energies such that the ions turn around at various locations. In certain aspects, such systems and methods for operating an ELIT may reduce ion density at the turning points to reduce the impact of the space charge effect. Various aspects of the present teachings also provide for the design or optimization of the ELIT electrode spacing and/or injection potentials to reduce the impact of the space charge effect. In some related aspects, the ELIT may additionally provide time-focusing of the various ion groups at the detector as they oscillate along their respective path lengths.
Described and claimed herein are capillary electrophoresis methods and kits for characterizing encapsulation efficiency or characterizing a biomolecule on an encapsulation.
In one aspect, a method of performing mass spectrometry is disclosed, which includes acquiring mass detection signals generated by an ion detector during an ion extraction event in a time-of-flight (ToF) mass analyzer in response to incidence of ions thereon, and applying an adjustable gain to the mass detection signals, wherein the step of applying the adjustable gain to the mass detection signals is performed dynamically based on m/z regions associated with said mass detection signals.
An automated method of operating a mass spectrometer (MS) comprising a differential mobility spectrometer (DMS) includes: introducing a first compound to the DMS; calculating a first alpha function for the first compound; introducing a second compound to the DMS; calculating a second alpha function for the second compound; and determining operation parameters of the DMS to achieve sufficient separation of the first compound and the second compound, based on the first alpha function and the second alpha function.
A mass spectrometer includes at least one ion optic for influencing the trajectory of at least one ion in an ionized sample. A casing includes a plurality of walls. Power supply components are disposed in the casing for supplying power to the at least one ion optic. An expansion component is within the casing. An encapsulant is disposed in the casing and encapsulates the power supply components. The encapsulant is in contact with the expansion component.
A method and system for sample processing, the system including an open port interface (OPI 104) comprising a removal conduit (125), the removal conduit comprising a removal conduit inlet and a removal conduit outlet and being configured to transport liquid between the (OPI 104) and a downstream device (120) via the removal conduit outlet, a fluid delivery pump (126) configured to provide a liquid flow to the OPI, a transfer capillary (302) in fluid communication with the removal conduit inlet, the transfer capillary (302) comprising a transfer capillary tip (312) located at a distance from the removal conduit inlet, and a distance adjusting device configured to adjust the distance between the transfer capillary tip and the removal conduit inlet.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
100.
SYSTEMS AND METHODS FOR IMPROVED SEQUENCE COVERAGE IN ANALYSIS OF LARGE POLYPEPTIDES
In some embodiments, a method for improved sequence coverage of a polypeptide comprises removing interchain disulfide bonds and retaining essentially all intrachain disulfide bonds of a first sample and performing a first mass spectrometry (MS) analysis of the first sample to determine a first amino acid sequence, removing interchain disulfide bonds and intrachain disulfide bonds of a second sample and performing a second MS analysis to determine a second amino acid sequence, and combining the first and second sequence to determine a combined amino acid sequence of the polypeptide.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
brevets
