An example method of the present disclosure comprises imbibing a Cannabaceae seed in a hydration solution, excising a subset of embryonic tissue from the imbibed Cannabaceae seed to extract a Cannabaceae meristematic region or embryonic axis (EA), and exposing the Cannabaceae meristematic region or EA to a heterologous nucleotide sequence to transform Cannabaceae cells of the Cannabaceae meristematic region or EA.
Embodiments of the present disclosure are directed to a plurality of nucleotide sequences encoding a first intein fused to at least a portion of a first transcription activator-like effector (TALE), a second nucleotide sequence encoding the first intein fused to at least a portion of a second TALE, and a third nucleotide sequence encoding a second intein fused to at least a portion of a rare-cutting nuclease.
Example embodiments in accordance with the present disclosure are directed to methods for transforming a plant part with a nucleotide sequence encoding an albumin and a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation, and culturing the plant part to enhance production of the albumin.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
Example embodiments in accordance with the present disclosure are directed to methods for contacting a plant part with a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation and a nucleotide sequence encoding an enzyme associated with production of a betalain, and culturing the plant part to enhance the production of the betalain.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C07K 14/415 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de végétaux
A61K 31/706 - Composés ayant des radicaux saccharide et des hétérocycles ayant l'azote comme hétéro-atome d'un cycle, p. ex. nucléosides, nucléotides contenant des cycles à six chaînons avec l'azote comme hétéro-atome d'un cycle
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
Example embodiments in accordance with the present disclosure are directed to methods comprising contacting a plant part with a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation, and culturing the plant part under growth conditions to enhance PCM formation.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
C07K 14/415 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de végétaux
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
Example embodiments in accordance with the present disclosure are directed to methods for transforming a Cannabaceae plant part by exposing the Cannabaceae plant part to an expression construct comprising a nucleotide sequence encoding an albumin to transform the Cannabaceae plant part with the expression construct, and inducing production of the albumin in the transformed Cannabaceae plant part.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
Example embodiments in accordance with the present disclosure are directed to methods for transforming a Cannabaceae plant part by exposing the Cannabaceae plant part to an expression construct comprising a nucleotide sequence encoding an enzyme associated with production of a betalain to transform the Cannabaceae plant part with the expression construct, and inducing production of the betalain in the transformed Cannabaceae plant part.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A61K 31/00 - Préparations médicinales contenant des ingrédients actifs organiques
A61K 31/706 - Composés ayant des radicaux saccharide et des hétérocycles ayant l'azote comme hétéro-atome d'un cycle, p. ex. nucléosides, nucléotides contenant des cycles à six chaînons avec l'azote comme hétéro-atome d'un cycle
A61K 33/00 - Préparations médicinales contenant des ingrédients actifs inorganiques
C07K 14/415 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de végétaux
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
8.
DELIVERY OF GENOME EDITING REAGENTS VIA CHIMERIC PLANTS
Embodiments of the present disclosure are directed to a method that includes generating a chimeric plant by infecting a cutting of a host plant with Rhizobium rhizogenes carrying an expression construct, and screening new growth from the chimeric plant for gene edits resulting from genomic editing by the expression construct.
Embodiments of the present disclosure are directed to a method for producing a grafted plant for delivery of genome editing reagents. The method may include grading cultured rootstock tissue to obtain at least one rootstock expressing an expression construct and having a stem with a graft-compatible diameter and genotype, and making a cut through the at least one rootstock stem and placing a stabilization device adjacent to the cut on the rootstock stem. The method may further include generating a grafted plant by inserting at least one cut scion stem into the stabilization device, wherein the at least one cut scion stem substantially aligns with vascular tissue within the cut rootstock stem, and screening new growth from the grafted plant for gene edits resulting from genomic editing by the expression construct
A method for rapid analysis of phytocannabinoids by near infrared spectroscopy includes generating a spectrum of diffuse reflection from a Cannabis sample, using a Fourier transform near infrared (FT-NIR) spectrometer. The method includes comparing the FT-NIR generated spectrum for the Cannabis sample to calibration data including previously-generated high performance liquid chromatography (HPLC) measurements for a plurality of Cannabis samples. The method further includes determining a plurality of biochemical characteristics of the Cannabis sample based on the comparison of the FT-NIR generated spectrum and the calibration data, and generating an output including the determined plurality of biochemical characteristics.
G01N 21/359 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière infrarouge en utilisant la lumière de l'infrarouge proche
G01N 33/94 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des narcotiques
G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
G01N 21/35 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière infrarouge
C12Q 1/6897 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques faisant intervenir des gènes rapporteurs liés de façon fonctionnelle à des promoteurs
Materials and methods are provided for making soybean varieties that have altered oil composition as a result of one or more mutation modulating the expression of a SACPD-C gene, a FATB-1A gene, or both the SACPD-C and FATB-1A genes. For example, a soybean plant, plant part, or plant cell producing an oil that has increased saturated fatty acid content as compared to oil produced from a corresponding soybean plant, plant part, or plant cell lacking the one or more mutations is provided.
The present disclosure relates to materials and methods for improving the oxidative stability and performance attributes of soybean oil. For example, provided are soybean oil compositions containing soybean oil derived from seed of a soybean plant comprising an induced deletion in at least one FAD2-1A allele and at least one FAD2-1B allele, having an oleic acid content of at least about 80% based on the weight of the total fatty acids of the oil, and having an oxidative stability index (OSI) value of greater than 25 hours to about 190 hours at 110° C.
Embodiments of the present disclosure are directed to a method that includes treating a cannabis plant, plant part, or plant cell with a solution including a chemical agent configured to modify a ploidy level of the cannabis plant, plant part, or plant cell. The method further includes, in response to the treatment, modifying the ploidy level of the cannabis plant, plant part, or plant cell.
TALEN compositions and methods of use are disclosed, which include using multiplexing compositions to create a targeted mutation in several genes at once, such as the FAD3 A/B/C genes, compositions to create a targeted mutation in a single gene, such as a gene encoding a FAD2 protein, and combinations thereof. The compositions and methods can provide gene-edited plants, plant parts, and plant cells that have improved characteristics compared to the corresponding unaltered plants, plant parts, or plant cells. For example, soybean plants, plant parts and plant cells that are capable of producing a seed with an oil having comparatively higher levels of oleic acid and lower levels of linoleic and linolenic acid than a corresponding seed lacking the targeted mutation are also provided.
Embodiments of the present disclosure are directed to a method that includes contacting a population of plant cells with a messenger ribonucleic acid (mRNA) construct including a sequence encoding a rare‑cutting endonuclease and a detectable label, wherein the rare‑cutting endonuclease is configured to induce a mutation at a target genomic locus. The method further includes screening the population of plant cells for the detectable label to identify target plant cells that are genetically transformed with the mRNA construct.
Gene-edited soybean plants, plant parts, and plant cells that have a reduced isoflavone content compared to the corresponding unaltered soybean plant, plant part, or plant cell and methods of their production are disclosed. For example, introducing one or more mutations in the coding region of Myb29 gene, the MYBCORE binding motif of the IFS2 promoter, the MYBCORE binding motif of the IFS1 promoter, the coding region of the IFS2 gene, the coding region of the IFS1 gene, or a combination thereof is used to produce the gene-edited plant, plant part or plant cell via targeted genome modification.
Materials and methods for clonal propagation of plants from plant cuttings using aeroponics are provided. For example, methods of seed increase including harvesting a plurality of cuttings from a single mother plant, rooting the cuttings in an aeroponics enclosure, and transferring the rooted cuttings to soil for reproductive development are provided. The methods can be used for any plants of interest. Advantages of the methods include reduced time and costs associated with generation of a full-grown plant from a cutting.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
29 - Viande, produits laitiers et aliments préparés ou conservés
30 - Aliments de base, thé, café, pâtisseries et confiseries
31 - Produits agricoles; animaux vivants
42 - Services scientifiques, technologiques et industriels, recherche et conception
45 - Services juridiques; services de sécurité; services personnels pour individus
Produits et services
(1) Chemical, biochemical, biotechnological and bacteriological products for use in industry, science, agriculture and horticulture, namely polypeptides for in vivo and in vitro genetic engineering, natural and engineered nucleases, nucleic acid vectors, molecular scissors; chemical, biochemical, biotechnological and bacteriological products for use in research laboratories and control laboratories, namely polypeptides for in vivo and in vitro genetic engineering, natural and engineered nucleases, nucleic acid vectors, molecular scissors; chemical, biochemical, biotechnological products and bacteriological preparations for science, namely polypeptides for in vivo and in vitro genetic engineering, natural and engineered nucleases, nucleic acid vectors, molecular scissors; seed gene for agricultural and horticultural production. Organic plant conditioners for use in agriculture and horticulture; potato, soya, corn, wheat, alfalfa and oat flour for industrial use.
(2) Pharmaceutical products, namely polypeptides for in vivo and in vitro genetic engineering natural and engineered nucleases, nucleic acid vectors, molecular scissors, preparations for fortifying plants and seed gene for use in the manufacture of agricultural or horticultural seeds; health food, beverages, preparations and substances, namely dietary supplements for general health and well-being, dietary supplements for promoting dietary fiber to aid digestion, dietary fibre as a food additive, vitamin preparations in the form of meal replacement drinks and powders, medical preparations and substances for treatment of malnutrition, cancer, high cholesterol, cardiovascular disease, diabetes, coronary heart disease, autoimmune diseases and immunologic deficiency syndromes; plant-based health-food substances, namely soy protein and soy oil dietary supplements, wheat germ dietary supplements, reduced-gluten wheat, high-fiber wheat, reduced trans-fat soybean oil, high-oleic soybean oil, high-oleic canola oil, and lower saturated fat canola oil; dietary and nutritional supplements, namely mineral supplements, nutritional supplement meal replacement bars for boosting energy; microorganism cultures and preparations for medical and pharmaceutical use namely biological tissue cultures for medical purposes for making healthier food substances in respect with medical purposes; bacteriological preparations for medical and pharmaceutical use, namely bacteriological culture mediums; biological preparations for medical and pharmaceutical use for treating cancer and malnutrition; chemical and biochemical preparations for medical and pharmaceutical use for treating cancer and malnutrition; diagnostic preparations for medical laboratory use; seeds and seedlings of genetically modified plants and vegetables for medical laboratory use.
(3) Edible oils and fats; processed fruits and vegetables, namely fruit chips, fruit jam, aromatized fruit, fermented vegetables; preserved, dried and cooked fruits and vegetables; snack food chips made from potatoes, soybeans, corn, wheat, alfalfa and oats; potato crisps, potato flakes and potato fritters, potato fries.
(4) Coffee, coffee substitutes; seasonings, sauces and condiments, namely mustard, hot sauce, soy sauce, tomato sauce, spices, herbs for food purposes, mayonnaise, ketchup; cooked ready meals, namely pasta side dishes, rice side dishes; cooked dish consisting primarily of potato, soya, corn, wheat, alfalfa and oat; soya, corn, wheat, alfalfa and oat crisps; soya, corn, wheat, alfalfa and oat flour and flakes; corn, soya, wheat, alfalfa and oat fries.
(5) Seeds for cultivation and agriculture; agricultural and horticultural seeds such as wheat seeds, corn seeds, oat seeds, sorghum seeds, barley seeds, soya seeds, seed potatoes, canola seeds, and tomato seeds; plant bulbs for agriculture and horticulture; seeds for growing vegetables, seeds for growing plants; fruit and vegetable seedlings; fresh fruits and vegetables. (1) Scientific and industrial research in the agri-food, horticulture and agriculture sector for the purposes of modifying the genome of any cell; genetic, medical and pharmaceutical research; research in chemistry, biology, biochemistry, molecular biology, and bacteriology; scientific analysis of modified seedlings and plants; research and development in techniques relating to biochemical analysis, biological analysis, and molecular biology; research and development in new compounds for use in the pharmaceutical industry, agriculture and horticulture; medical laboratory services; technical project studies and appraisals (engineering) whose purpose is the modification of the genome of any cell within the agri-food, horticulture and agriculture sector; licensing of patents and intellectual property; genetic engineering, namely, gene editing of plants intended to agriculture and horticulture.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
29 - Viande, produits laitiers et aliments préparés ou conservés
30 - Aliments de base, thé, café, pâtisseries et confiseries
31 - Produits agricoles; animaux vivants
42 - Services scientifiques, technologiques et industriels, recherche et conception
45 - Services juridiques; services de sécurité; services personnels pour individus
Produits et services
(1) Chemical, biochemical, biotechnological and bacteriological products for use in industry, science, agriculture and horticulture, namely polypeptides for in vivo and in vitro genetic engineering, natural and engineered nucleases, nucleic acid vectors, molecular scissors; chemical, biochemical, biotechnological and bacteriological products for use in research laboratories and control laboratories, namely polypeptides for in vivo and in vitro genetic engineering, natural and engineered nucleases, nucleic acid vectors, molecular scissors; chemical, biochemical, biotechnological products and bacteriological preparations for science, namely polypeptides for in vivo and in vitro genetic engineering, natural and engineered nucleases, nucleic acid vectors, molecular scissors; seed gene for agricultural and horticultural production. Organic plant conditioners for use in agriculture and horticulture; potato, soya, corn, wheat, alfalfa and oat flour for industrial use.
(2) Pharmaceutical products, namely polypeptides for in vivo and in vitro genetic engineering natural and engineered nucleases, nucleic acid vectors, molecular scissors, preparations for fortifying plants and seed gene for use in the manufacture of agricultural or horticultural seeds; health food, beverages, preparations and substances, namely dietary supplements for general health and well-being, dietary supplements for promoting dietary fiber to aid digestion, dietary fibre as a food additive, vitamin preparations in the form of meal replacement drinks and powders, medical preparations and substances for treatment of malnutrition, cancer, high cholesterol, cardiovascular disease, diabetes, coronary heart disease, autoimmune diseases and immunologic deficiency syndromes; plant-based health-food substances, namely soy protein and soy oil dietary supplements, wheat germ dietary supplements, reduced-gluten wheat, high-fiber wheat, reduced trans-fat soybean oil, high-oleic soybean oil, high-oleic canola oil, and lower saturated fat canola oil; dietary and nutritional supplements, namely mineral supplements, nutritional supplement meal replacement bars for boosting energy; microorganism cultures and preparations for medical and pharmaceutical use namely biological tissue cultures for medical purposes for making healthier food substances in respect with medical purposes; bacteriological preparations for medical and pharmaceutical use, namely bacteriological culture mediums; biological preparations for medical and pharmaceutical use for treating cancer and malnutrition; chemical and biochemical preparations for medical and pharmaceutical use for treating cancer and malnutrition; diagnostic preparations for medical laboratory use; seeds and seedlings of genetically modified plants and vegetables for medical laboratory use.
(3) Edible oils and fats; processed fruits and vegetables, namely fruit chips, fruit jam, aromatized fruit, fermented vegetables; preserved, dried and cooked fruits and vegetables; snack food chips made from potatoes, soybeans, corn, wheat, alfalfa and oats; potato crisps, potato flakes and potato fritters, potato fries.
(4) Coffee, coffee substitutes; seasonings, sauces and condiments, namely mustard, hot sauce, soy sauce, tomato sauce, spices, herbs for food purposes, mayonnaise, ketchup; cooked ready meals, namely pasta side dishes, rice side dishes; cooked dish consisting primarily of potato, soya, corn, wheat, alfalfa and oat; soya, corn, wheat, alfalfa and oat crisps; soya, corn, wheat, alfalfa and oat flour and flakes; corn, soya, wheat, alfalfa and oat fries.
(5) Seeds for cultivation and agriculture; agricultural and horticultural seeds such as wheat seeds, corn seeds, oat seeds, sorghum seeds, barley seeds, soya seeds, seed potatoes, canola seeds, and tomato seeds; plant bulbs for agriculture and horticulture; seeds for growing vegetables, seeds for growing plants; fruit and vegetable seedlings; fresh fruits and vegetables. (1) Scientific and industrial research in the agri-food, horticulture and agriculture sector for the purposes of modifying the genome of any cell; genetic, medical and pharmaceutical research; research in chemistry, biology, biochemistry, molecular biology, and bacteriology; scientific analysis of modified seedlings and plants; research and development in techniques relating to biochemical analysis, biological analysis, and molecular biology; research and development in new compounds for use in the pharmaceutical industry, agriculture and horticulture; medical laboratory services; technical project studies and appraisals (engineering) whose purpose is the modification of the genome of any cell within the agri-food, horticulture and agriculture sector; licensing of patents and intellectual property; genetic engineering, namely, gene editing of plants intended to agriculture and horticulture.
