Abrégé

The invention relates to the field of cell and tissue culture. In particular, the invention relates to devices for cell culture. A device for cell culture, according to the invention comprises (i) a medium conduit having one open end and one end closed by a porous membrane fused across it; and (ii) a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane, characterised in that the surface of the membrane contralateral to the surface of said porous membrane sealed to said medium conduit comprises a hydrophobic barrier adapted to contain the culture.

Classes IPC  ?

• C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
• C12M 1/20 - Champs plans horizontaux

Abrégé

The invention relates to the field of cell and tissue culture. In particular, the invention provides methods for culturing cells to form aggregates, including stem cells and primary cells. A method for culturing cells according to the invention comprises the steps of: (i) incubating a cells in a hanging drop on the underside of a porous membrane to form aggregates of cells; (ii) inverting the membrane so that the aggregates of cells are located on the upperside of the membrane; and (iii) incubating the aggregates of cells on the upperside of the membrane.

Classes IPC  ?

• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
• C12N 5/077 - Cellules mésenchymateuses, p. ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
• C12N 5/0793 - Neurones