Methods and systems for processing semen samples from straws for determining genetic identity, testing the purity of the sample, detecting errors or contamination, calculating an amount of contamination, and determining the identity of the contaminant. The methods herein can detect low levels of contamination, such as contamination of about 0.5%, 1%, 2%, etc.
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
4.
METHODS AND COMPOSITIONS FOR TARGETED GENETIC INACTIVATION OF PRIMORDIAL GERM CELL (PGC) SPECIFICATION GENES FOR EFFICIENT GERMLINE ABLATION AND EXCLUSIVE DONOR CELL GERMLINE TRANSMISSION IN GENE-EDITED ANIMALS
NANOSNANOS for efficient germline ablation and exclusive donor cell germline transmission in non-human mammals. The application of this technique in the agricultural industry holds significant promise for accelerating the propagation of desirable traits in agricultural animals and catalyzing the efficient use of valuable genetic resources and the creation of genetically uniform herds, thereby improving the overall sustainability and profitability of livestock farming.
C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
C12N 5/073 - Cellules ou tissus embryonnairesCellules fœtales ou tissus fœtaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Animal embryos; bovine embryos; genetic animal breeding material, namely embryos Scientific and technological services in the field of animal genetics and embryo production; development of embryo production systems; technology transfer services in the field of animal reproduction; technical consulting relating to livestock genetic technologies Animal breeding; embryo transfer services; artificial insemination services; in vitro fertilization services for animals; veterinary and reproductive services for livestock; animal breeding and reproductive management consulting services; Providing information relating to animal breeding and livestock reproductive management
6.
SYSTEMS, METHODS, AND APPARATUS FOR SEXING SPERM BY ELECTRICAL CHARGE INTERACTION AND RHEOTAXIS
Disclosed herein is process and method for sexing non-human animal sperm in using a microfluidic chip utilizing the electrical charge interaction between the sperm cells and the chip and rheotaxis. In some examples, a non-human animal semen sample is collected and loaded into a two chamber microfluidic chip, wherein the two chambers are connected by a plurality of microchannels. In some examples, the non-human animal semen sample can be sexed for one or both of X chromosome or Y chromosome-bearing sperm cells from a single sample. In some examples, magnetic beads are used to improve differentiation between X and Y chromosome-bearing sperm cells.
Systems and methods are provided for identification and discrimination of subpopulations within a mixture of particles. The systems and methods implement continuous calibration of the classification of particles within the mixture to provide consistency in operation and to reduce inter- and intra-batch processing variation. The systems and methods produce advantageously sorted particle products.
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
G01N 15/10 - Recherche de particules individuelles
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/149 - Techniques de recherche optique, p. ex. cytométrie en flux spécialement adaptées au tri des particules, p. ex. selon leur taille ou leurs propriétés
The present disclosure relates to methods of creating Porcine Reproductive and Respiratory Syndrome virus (PRRSv) resistant pigs by gene silencing. In particular, the methods comprise using tools such as CRISPR/CAS9 to edit genes that either directly encode or encode molecules that are processed into non-coding RNA molecules to induce or modify their silencing activity against CD163 and/or in cells of pigs for the purpose of promoting PRRSv resistance.
Disclosed is an approach to differentiating between different particle types in samples flowing through microfluidics chips. A sample may have an initial proportion of a first cell type to a second cell type. An illuminating light source may emit a coherent light at the sample, and light leaving the chip in a first direction may be detected using a first light detector, and light leaving the chip in a second direction (e.g., orthogonal to the first direction) may be detected using a second light detector. The detected light may be fluorescence. An orientational feature of a plurality of cells in the sample may be determined based on the light detected by the detectors. Based on the orientational features and the detected light, a biasing operation may be performed for each cell in the sample to obtain a modified proportion of cell types in the sample.
Disclosed herein is a microfluidic chip for positioning samples for particle tracing. In some examples, the microfluidic chip includes a first microchannel and a second microchannel. The first microchannel and the second microchannel are mated to form a junction, with a lateral distance of the first microchannel being less than a lateral distance of the second microchannel.
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
The present invention provides rapid genomic diagnostic methods of in vitro produced embryos. Cell-free DNA can be obtained from the culture medium of blastocysts, and then added to a LAMP or PCR amplification reaction to detect the presence or absence of a certain DNA sequence. Colorimetric or fluorescent indicators such as metallochromatic dyes, intercalating dyes, or pH indicator dyes can be added to the reaction so that amplification can be tracked without additional steps. Provided are methods of sex determination and detection of a specific gene edit, as well adverse or desirable phenotypes. Methods of genotyping using cell-free DNA are also provided.
C12N 15/873 - Techniques de production de nouveaux embryons, p. ex. transfert nucléaire, manipulation de cellules totipotentes ou production d'embryons chimériques
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
The present disclosure relates generally to methods for improving traits or fitness in cattle, pigs, or aquatic organisms via gene editing. In particular, the methods comprise modifying genes in embryonic stem cells of livestock (e.g., cattle, pigs, or fish) for the purpose of improving a trait (e.g., promoting pathogen resistance, fertility, lactation, and native traits that support more rapid growth or feed efficiency). The methods particularly include CRISPR-CAS9 editing of porcine and bovine embryonic stem cells. Also disclosed herein are methods of producing and cultivating porcine embryonic stem cells under feeder free conditions.
C12N 15/873 - Techniques de production de nouveaux embryons, p. ex. transfert nucléaire, manipulation de cellules totipotentes ou production d'embryons chimériques
The present teachings provide for a method of breeding livestock in vitro. Provided are steps to create embryonic stem cells from a plurality of blastocysts, genotype the embryonic stem cells to select the best embryos for mating to create offspring, and induce the embryonic stem cells into primordial germ cell-like cells (PGCLCs). Male PGCLCs are further induced into spermatogonial stem cell-like cells, and then spermatid-like cells. Female PGCLCs are induced into oocytes, which are then matured. The resulting gametes can then be mixed with each other or with opposite sex gametes from animals with desirable genetics to create the next generation of embryos, which can then be run through the process again. This method of in vitro breeding can be used to increase the speed of genetic progress in livestock.
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
Modular flow cytometry systems and methods for processing samples are described herein. The systems include automated or semi-automated modules that are replaceable and removable. A sample pathway module may be removed and placed in a microfluidic device cleaning module for cleaning, and then reinstalled or stored for later use. The systems further include optical modules, electronics modules, and mixing and collection modules. The optical module includes a photo-damaging assembly and detection laser assembly that may be on the same side relative to a plane or surface of a flow cytometry device and opposite of a detection assembly. The laser beam may have a beam waist that is wider in a direction perpendicular to a flow direction than in the flow direction. The mixing and collection module can automatically mix a sample being collected in a sample tube and switch to another sample tube when the other tube is full.
Disclosed are gene edited ungulate animals and methods of editing genes that control various ungulate traits. Such methods include CRISPR, zinc fingers, or TALENS. Exemplary traits for editing include polled, sterility or fertility, milk production, growth (which increases meat production), fat production, conception rates, stillborn rates, calving ease, or content of produced milk such as the amount of protein or the amount of fat. Other traits include backfat thickness, intramuscular fat, ultrasound loin muscle area, loin muscle area and intramuscular fat content, chest circumference, withers height, body length, hip height, rump length, and heart girth. Other exemplary native traits include, but are not limited to, high altitude adaptation and response to hypoxia, cold acclimation, body size and stature, resistance to disease and bacterial infection, reproduction, milk yield and components, and feed efficiency.
What is provided is a microweir structure or a set of one or more microweir structures disposed in a microfluidic flow channel, such as a channel in one or more substrate layers of a microfluidic chip. The microweir structure or set of microweir structures orient particles suspended in a sample fluid stream in the microfluidic flow channel, or focus a sample stream which is in laminar flow with a buffer fluid stream in the microfluidic flow channel. A set of one or more lower pressure zones or vortices downstream of a microweir structure contribute to the focusing and orientation effects of the microweir structures.
A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells. By looking solely at properties measured within the multi-cell fluorescent event, a decision of which cell to target for elimination can be made. Using this method with large population sizes can result in an effective sex skewed product. The sex skewed product can, for example, be formed from bull semen which is then later used to inseminate cows which results in an increased likelihood of giving birth to female cattle.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
Disclosed are methods of increasing production of sex selected semen for elite animals without a loss of plant efficiency. Assignment of elite animal ejaculate to a number of instruments so that a set number of cells is applied to each instrument is disclosed. Also disclosed is allowing the instrument to run until the ejaculate sample is exhausted, the instrument reports a dead percent of 25, or the sample has been processing for eight hours. Methods of pairing elite bulls with less valuable animals to preserve plant efficiency are also disclosed.
Provided herein are methods of producing heterozygous edited animals by injecting zygotes with low amounts of ribonucleoprotein (RNP) complex. The data cited herein show that more heterozygous animals are obtained from zygotes injected with 100,000 – 450,000, or approximately 111,250 copies of an RNP complex. The methods disclosed herein are particularly useful for editing of bovine or porcine zygotes. Heterozygous animals are preferred when homozygous embryos fail to develop, or when homozygous animals are sterile, therefore making it difficult to breed the edit into a line of animals.
The present teachings provide for methods of separating live and dead spermatozoa cells from non-human mammalian sex-selected semen. Sex-selected semen is first treated with proteinase K and TCEP, then applied to a density gradient media for separation. Separation is typically accomplished through centrifugation. Commercially available density gradient media include PERCOLL® and BOVIPURE®. Separation of live and dead cells in sex selected semen allows for precise quantification of sex skew using ddPCR™.
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
25.
PRODUCTION OF LIVESTOCK ANIMALS FROM EMBRYONIC STEM CELLS
Disclosed are methods of creating embryonic stem cells through nuclear transfer or embryo complementation. In general, methods of complementation include contacting embryonic stem cells with a host embryo. Host embryos can be wild-type embryos, tetraploid embryos, parthenogenetic embryos, trophoblastic vesicles, or embryos that have been gene edited to prevent them from making germ cells. Trophoblastic vesicles are typically made by ablating the inner cell mass of an embryo via heat shock, chemical treatment, laser ablation, or microdissection of the ICM. The embryonic stem cells can then be injected into the host embryo or fused with the host embryo by placing them in contact in culture. Once fused and matured, the resulting mosaic embryo is then implanted into a surrogate mother.
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
C12N 15/873 - Techniques de production de nouveaux embryons, p. ex. transfert nucléaire, manipulation de cellules totipotentes ou production d'embryons chimériques
C12N 15/877 - Techniques de production de nouveaux embryons clonés de mammifères
05 - Produits pharmaceutiques, vétérinaires et hygièniques
29 - Viande, produits laitiers et aliments préparés ou conservés
31 - Produits agricoles; animaux vivants
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Animal semen
(2) Meat
(3) Animal embryos; cattle; live cattle
(4) Dairy cows; (1) Animal husbandry; artificial insemination services; artificial insemination services for animals; cattle breeding services; information, advice and consultancy services in the field of artificial insemination services for animals
(2) Dairy cow breeding services; Consultancy and advisory services in the field of animal breeding and animal reproductive management;
27.
Methods of sperm cell sensing utilizing a semiconductor detector and cytometer apparatus
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
H01L 31/107 - Dispositifs sensibles au rayonnement infrarouge, visible ou ultraviolet caractérisés par une seule barrière de potentiel ou de surface la barrière de potentiel fonctionnant en régime d'avalanche, p.ex. photodiode à avalanche
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/1404 - Manipulation du flux, p. ex. focalisation hydrodynamique
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
An integrated system and method for preparing sperm cells to improve their survivability during cryopreservation are described herein. The system features a vessel, a controlled dispenser, and a dispense tube. The dispense tube has a first end fluidly connected to the dispenser and a second end disposed inside the vessel. The second end can be submerged in the sperm cell fluid. The controlled dispenser may be a syringe pump that includes a syringe for containing a cryoprotectant and a pushing mechanism for displacing the syringe. The syringe pump is configured to discharge the cryoprotectant through the dispense tube and into the vessel, thereby dispensing the cryoprotectant into the sperm cell fluid. Mixing of the fluids is achieved using a shaker table agitation.
Provided are genetically edited animals, particularly dairy animals such as cows or heifers, that lactate without having first been pregnant. Also provided are methods and reagents for creating genetically modified animals. Specifically provided are genetically edited dairy animals that have a histidine to arginine substitution in the prolactin receptor gene.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Live animals; Animal embryos Animal semen; animal healthcare products, namely, teat dip and bactericidal or germicidal preparations for domestic animals Artificial insemination services; consultancy and advisory services in the field of animal breeding and reproductive management
31.
SYSTEMS, METHODS, AND APPARATUS FOR A MICROFLUIDIC CHIP HAVING A MICROCHANNEL DESIGN WHICH ASYMMETRICALLY FOCUSES PARTICLES
What is provided is an asymmetrically focusing microfluidic chip and a method of asymmetrically focusing and processing particles using an asymmetrically focusing microfluidic chip. The microfluidic chip comprises a microfluidic channel structure with a sheath fluid channel structure originating upstream at a sheath fluid inlet, the sheath fluid channel structure comprising a first side channel structure and a second side channel structure each having a plurality of tines. A sheath fluid flows through the sheath fluid channel structure into a flow channel at an intersection with a sample fluid flowing from a sample fluid channel. The sample fluid is asymmetrically focused in the intersection. The sample fluid may be analyzed and processed before flowing out of the channel structure via one or more outlets.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
29 - Viande, produits laitiers et aliments préparés ou conservés
31 - Produits agricoles; animaux vivants
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Animal semen. Meat. Live animals, namely cattle and dairy cows; animal embryos. Artificial insemination services; animal breeding; consultancy and advisory services in the field of animal breeding and reproductive management.
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure relates generally to potassium channel blocker compounds and compositions thereof; and methods for improving reproductive cell viability and quality, during and/or after one or more of staining, freezing, thawing, cell sample enrichment, packaging, or in vitro fertilization using the potassium channel blocker compounds.
A61K 31/14 - Composés d'ammonium quaternaire, p. ex. édrophonium, choline
A61P 15/08 - Médicaments pour le traitement des troubles génitaux ou sexuelsContraceptifs pour les troubles gonadiques ou pour augmenter la fertilité, p. ex. inducteurs d'ovulation ou de spermatogénèse
e.g.in vitroin vitro fertilization using the fertilization enhancing compounds. These compounds are especially useful for preserving sperm cell viability during a sex selection or sexing process.
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic devices feature a microfluidic chip having a micro-channel having a constricting portion that narrows in width, and a flow focusing region downstream of the micro-channel. The flow focusing region includes a positively sloping bottom surface that reduces a height of the flow focusing region and sidewalls that taper to reduce a width of the flow focusing region, thereby geometrically constricting the flow focusing region. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.
e.g.,e.ge.g., promoting pathogen resistance, fertility, lactation, and native traits that support more rapid growth or feed efficiency). The methods particularly include CRISPR-CAS9 editing of porcine and bovine embryonic stem cells. Also disclosed herein are methods of producing and cultivating porcine embryonic stem cells under feeder free conditions.
in vitro in vitro produced embryos. Cell-free DNA can be obtained from the culture medium of blastocysts, and then added to a LAMP or PCR amplification reaction to detect the presence or absence of a certain DNA sequence. Colorimetric or fluorescent indicators such as metallochromatic dyes, intercalating dyes, or pH indicator dyes can be added to the reaction so that amplification can be tracked without additional steps. Provided are methods of sex determination and detection of a specific gene edit, as well adverse or desirable phenotypes. Methods of genotyping using cell-free DNA are also provided.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Computer hardware for tracking livestock location and health; Computer software for tracking livestock location and health; Computer software applications for tracking livestock location and health; Downloadable mobile application for tracking livestock location and health. Software as a service (SAAS) services for use in the tracking, analysis, and reporting of livestock location and health. Consulting services related to livestock management, performance, and health.
43.
COMPUTATIONAL TECHNIQUES FOR IDENTIFYING UNOBSERVED HEREDITARY INFORMATION BASED ON ANALYSIS OF LIMITED DATA
Systems and methods for organism genotyping and using genomic data for genotype imputation are disclosed. The system can maintain first genetic sequence information of a sire and second genetic sequence information for a dam, the first and second genetic sequence information indicating one or more single nucleotide polymorphisms (SNPs) of interest. The system can sequence, based on a skim sequencing technique, a sample of genetic information of a progeny of the sire and the dam. The system can identify informative variants based on the first genetic sequence information and the second genetic sequence information. The system can identify informative reads in the sequence of the progeny based on the informative variants. The system can construct a genotype of the progeny based on the informative reads and the one or more SNPs of interest in the first and second genetic sequence information.
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
H01L 31/107 - Dispositifs sensibles au rayonnement infrarouge, visible ou ultraviolet caractérisés par une seule barrière de potentiel ou de surface la barrière de potentiel fonctionnant en régime d'avalanche, p.ex. photodiode à avalanche
H01L 31/02 - Dispositifs à semi-conducteurs sensibles aux rayons infrarouges, à la lumière, au rayonnement électromagnétique d'ondes plus courtes, ou au rayonnement corpusculaire, et spécialement adaptés, soit comme convertisseurs de l'énergie dudit rayonnement e; Procédés ou appareils spécialement adaptés à la fabrication ou au traitement de ces dispositifs ou de leurs parties constitutives; Leurs détails - Détails
H01L 31/024 - Dispositions pour le refroidissement, le chauffage, la ventilation ou la compensation de température
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Computer hardware for tracking livestock location and health; Computer software for tracking livestock location and health; Computer software applications for tracking livestock location and health; Downloadable mobile application for tracking livestock location and health. Software as a service (SAAS) services for use in the tracking, analysis, and reporting of livestock location and health. Consulting services related to livestock management, performance, and health.
A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells. By looking solely at properties measured within the multi-cell fluorescent event, a decision of which cell to target for elimination can be made. Using this method with large population sizes can result in an effective sex skewed product. The sex skewed product can, for example, be formed from bull semen which is then later used to inseminate cows which results in an increased likelihood of giving birth to female cattle.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
F04B 19/00 - "Machines" ou pompes ayant des caractéristiques particulières non prévues dans les groupes ou présentant un intérêt autre que celui visé par ces groupes
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
48.
METHODS FOR IMPROVING REPRODUCTIVE CELL SAMPLE QUALITY
The present disclosure relates generally to methods of improving reproductive cell sample quality. The methods include improving the quality of mammalian semen cell samples by discriminating between live and dead sperm cells in a semen sample obtained from livestock, such as bovine or porcine animals. The methods include improving reproductive cell sample quality based on the exposure or availability of an acrosome associated molecule by contacting the sample with a binding agent that binds to the acrosome associated molecule to form a mixture of the semen and the binding agent and separating sperm cells bound to the binding agent from sperm cells not bound to the binding agent.
C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
C12Q 3/00 - Procédés de commande sensible aux conditions du milieu
G01N 15/00 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux
49.
Microfluidic system and method with focused energy apparatus
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
The present teachings provide for a method of breeding livestock in vitro. Provided are steps to create embryonic stem cells from a plurality of blastocysts, genotype the embryonic stem cells to select the best embryos for mating to create offspring, and induce the embryonic stem cells into primordial germ cell-like cells (PGCLCs). Male PGCLCs are further induced into spermatogonial stem cell-like cells, and then spermatid-like cells. Female PGCLCs are induced into oocytes, which are then matured. The resulting gametes can then be mixed with each other or with opposite sex gametes from animals with desirable genetics to create the next generation of embryos, which can then be run through the process again. This method of in vitro breeding can be used to increase the speed of genetic progress in livestock.
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
Disclosed is an approach to differentiating between different particle types in samples flowing through microfluidics chips. A sample may have an initial proportion of a first cell type to a second cell type. An illuminating light source may emit a coherent light at the sample, and light leaving the chip in a first direction may be detected using a first light detector, and light leaving the chip in a second direction (e.g., orthogonal to the first direction) may be detected using a second light detector. The detected light may be fluorescence. An orientational feature of a plurality of cells in the sample may be determined based on the light detected by the detectors. Based on the orientational features and the detected light, a biasing operation may be performed for each cell in the sample to obtain a modified proportion of cell types in the sample.
Disclosed is an approach to differentiating between different particle types in samples flowing through microfluidics chips. A sample may have an initial proportion of a first cell type to a second cell type. An illuminating light source may emit a coherent light at the sample, and light leaving the chip in a first direction may be detected using a first light detector, and light leaving the chip in a second direction (e.g., orthogonal to the first direction) may be detected using a second light detector. The detected light may be fluorescence. An orientational feature of a plurality of cells in the sample may be determined based on the light detected by the detectors. Based on the orientational features and the detected light, a biasing operation may be performed for each cell in the sample to obtain a modified proportion of cell types in the sample.
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
H01L 31/107 - Dispositifs sensibles au rayonnement infrarouge, visible ou ultraviolet caractérisés par une seule barrière de potentiel ou de surface la barrière de potentiel fonctionnant en régime d'avalanche, p.ex. photodiode à avalanche
H01L 31/024 - Dispositions pour le refroidissement, le chauffage, la ventilation ou la compensation de température
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
Provided are genetically edited animals, particularly dairy animals such as cows or heifers, that lactate without having first been pregnant. Also provided are methods and reagents for creating genetically modified animals. Specifically provided are genetically edited dairy animals that have a histidine to arginine substitution in the prolactin receptor gene.
A microfluidic system configured to focus particles suspended in a fluid. One general aspect includes a microfluidic system comprising one or more substrates and a focusing channel formed in the one or more substrates and spanning a length from an inlet to an outlet for receiving a flow of particles suspended in fluid, wherein the particles have a diameter (a) and the focusing channel has a hydraulic diameter (dh).
Modular flow cytometry systems and methods for processing samples are described herein. The systems include automated or semi-automated modules that are replaceable and removable. A sample pathway module may be removed and placed in a microfluidic device cleaning module for cleaning, and then reinstalled or stored for later use. The systems further include optical modules, electronics modules, and mixing and collection modules. The optical module includes a photo-damaging assembly and detection laser assembly that may be on the same side relative to a plane or surface of a flow cytometry device and opposite of a detection assembly. The laser beam may have a beam waist that is wider in a direction perpendicular to a flow direction than in the flow direction. The mixing and collection module can automatically mix a sample being collected in a sample tube and switch to another sample tube when the other tube is full.
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
G01N 15/00 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux
61.
Modular flow cytometry systems and methods of processing samples
Modular flow cytometry systems and methods for processing samples are described herein. The systems include automated or semi-automated modules that are replaceable and removable. A sample pathway module may be removed and placed in a microfluidic device cleaning module for cleaning, and then reinstalled or stored for later use. The systems further include optical modules, electronics modules, and mixing and collection modules. The optical module includes a photo-damaging assembly and detection laser assembly that may be on the same side relative to a plane or surface of a flow cytometry device and opposite of a detection assembly. The laser beam may have a beam waist that is wider in a direction perpendicular to a flow direction than in the flow direction. The mixing and collection module can automatically mix a sample being collected in a sample tube and switch to another sample tube when the other tube is full.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Animal breeding, artificial insemination of animals, animal
semen extraction, animal sperm bank services; animal stud
services; veterinary services; genetic testing of animals;
advisory, information and consultancy services related to
all the aforesaid services.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Animal breeding, artificial insemination of animals, animal
semen extraction, animal sperm bank services; animal stud
services; veterinary services; genetic testing of animals;
advisory, information and consultancy services related to
all the aforesaid services.
64.
METHODS AND SYSTEMS FOR PROCESSING GENETIC SAMPLES TO DETERMINE IDENTITY OR DETECT CONTAMINATION
Methods and systems for processing semen samples from straws for determining genetic identity, testing the purity of the sample, detecting errors or contamination, calculating an amount of contamination, and determining the identity of the contaminant. The methods herein can detect low levels of contamination, such as contamination of about 1%, 2%, etc.
Methods and systems for processing semen samples from straws for determining genetic identity, testing the purity of the sample, detecting errors or contamination, calculating an amount of contamination, and determining the identity of the contaminant. The methods herein can detect low levels of contamination, such as contamination of about 0.5%, 1%, 2%, etc.
The present disclosure relates generally to methods for using porcine sex-sorted sperm cells for the efficient dissemination of desirable traits in multi-level swine production systems. The methods include using sex-sorted sperm cells for skewing offspring gender at the commercial farm level, producing porcine herds having improved growth performance traits, producing pathogen-resistant porcine herds, and disseminating desirable traits from a genetic nucleus to commercial farms using low dose artificial insemination techniques. The methods also provide a means for reducing costs at the production level by increasing the ratio of female offspring and improving animal welfare at all levels of production by reducing or eliminating male castration. In addition, the methods of the present technology may be employed to develop production flows for specialized pork products.
A61D 19/02 - Instruments ou procédés pour la reproduction ou la fertilisation pour l'insémination artificielle
A61K 35/52 - SpermeProstateLiquide séminalCellules de Leydig des testicules
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Animal breeding, artificial insemination of animals, animal semen extraction for animal breeding purposes, animal sperm bank services; animal stud services; veterinary services; genetic testing of animals for diagnostic or breeding planning purposes; advisory, information and consultancy services related to all the aforesaid services
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Agricultural services, namely animal breeding, artificial insemination of animals, animal semen extraction, animal sperm bank services; stud services for cattle; veterinary services; genetic testing of animals; advisory, information and consultancy services related to the breeding of cattle.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Agricultural services, namely animal breeding, artificial insemination of animals, animal semen extraction, animal sperm bank services; stud services for cattle; veterinary services; genetic testing of animals; advisory, information and consultancy services related to the breeding of cattle.
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Animal breeding, artificial insemination of animals, animal semen extraction for animal breeding purposes, animal sperm bank services; animal stud services; veterinary services; genetic testing of animals for diagnostic or breeding planning purposes; advisory, information and consultancy services related to all the aforesaid services
71.
System and methods for sub-population identification within a mixture of particles based on critical ranges of values
Systems and methods are provided for identification and discrimination of subpopulations within a mixture of particles. The systems and methods implement continuous calibration of the classification of particles within the mixture to provide consistency in operation and to reduce inter- and intra-batch processing variation. The systems and methods produce advantageously sorted particle products.
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
G01N 15/10 - Recherche de particules individuelles
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/149 - Techniques de recherche optique, p. ex. cytométrie en flux spécialement adaptées au tri des particules, p. ex. selon leur taille ou leurs propriétés
72.
Microfluidic system and method with focused energy apparatus
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
F04B 19/00 - "Machines" ou pompes ayant des caractéristiques particulières non prévues dans les groupes ou présentant un intérêt autre que celui visé par ces groupes
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
G01N 15/10 - Recherche de particules individuelles
Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic devices feature a microfluidic chip having a micro-channel having a constricting portion that narrows in width, and a flow focusing region downstream of the micro-channel. The flow focusing region includes a positively sloping bottom surface that reduces a height of the flow focusing region and sidewalls that taper to reduce a width of the flow focusing region, thereby geometrically constricting the flow focusing region. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.
Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic devices feature a microfluidic chip having a micro-channel having a constricting portion that narrows in width, and a flow focusing region downstream of the micro-channel. The flow focusing region includes a positively sloping bottom surface that reduces a height of the flow focusing region and sidewalls that taper to reduce a width of the flow focusing region, thereby geometrically constricting the flow focusing region. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.
Technologies for assessing, quantifying and isolating sperm cell populations and/or subpopulations having specific genetic signatures are provided, as well as methods and systems to assess the efficacy of chromosomal differentiation processes. Compositions for identification and differentiation of X-chromosomes and Y-chromosomes in DNA are also provided.
The present teachings provide for and include methods of preparing semen for in vitro fertilization (IVF). Provided are methods of removing dead cells from semen preparations by applying the semen to a colloidal silica gradient and centrifuging the gradient. In some configurations, the gradient is a 45-90% colloidal silica gradient and the centrifugation is at 419 x g. Further provided are use of the method of the present teachings for processing sex selected semen. Also provided are ova and zygotes matured therefrom that have been fertilized by semen that has been processed by a method of the present teachings.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
78.
Methods of sperm cell sensing utilizing an avalanche photodiode and cytometer apparatus
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
H01L 31/107 - Dispositifs sensibles au rayonnement infrarouge, visible ou ultraviolet caractérisés par une seule barrière de potentiel ou de surface la barrière de potentiel fonctionnant en régime d'avalanche, p.ex. photodiode à avalanche
H01L 31/024 - Dispositions pour le refroidissement, le chauffage, la ventilation ou la compensation de température
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
Bos taurus variety JE840003146074527. Included in the present disclosure are cells comprising the Bovine variety JE840003146074527. Also provided by the present disclosure are tissue cultures of cells, animals obtained from said cells, and parts thereof, including F1 spermatozoa. The disclosure further provides for methods of breeding, selecting, and using the germplasm to improve existing commercial cattle herds generated from in vitro fertilization methods and progeny cattle obtained from in vitro fertilization and implantation and artificial insemination methods.
A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells. By looking solely at properties measured within the multi-cell fluorescent event, a decision of which cell to target for elimination can be made. Using this method with large population sizes can result in an effective sex skewed product. The sex skewed product can, for example, be formed from bull semen which is then later used to inseminate cows which results in an increased likelihood of giving birth to female cattle.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
An integrated system and method for preparing sperm cells to improve their survivability during cryopreservation are described herein. The system features a vessel, a controlled dispenser, and a dispense tube. The dispense tube has a first end fluidly connected to the dispenser and a second end disposed inside the vessel. The second end can be submerged in the sperm cell fluid. The controlled dispenser may be a syringe pump that includes a syringe for containing a cryoprotectant and a pushing mechanism for displacing the syringe. The syringe pump is configured to discharge the cryoprotectant through the dispense tube and into the vessel, thereby dispensing the cryoprotectant into the sperm cell fluid. Mixing of the fluids is achieved using a shaker table agitation.
A61B 18/02 - Instruments, dispositifs ou procédés chirurgicaux pour transférer des formes non mécaniques d'énergie vers le corps ou à partir de celui-ci par refroidissement, p. ex. techniques cryogéniques
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
86.
System and process for continuous addition of cryoprotectant
An integrated system and method for preparing sperm cells to improve their survivability during cryopreservation are described herein. The system features a vessel, a controlled dispenser, and a dispense tube. The dispense tube has a first end fluidly connected to the dispenser and a second end disposed inside the vessel. The second end can be submerged in the sperm cell fluid. The controlled dispenser may be a syringe pump that includes a syringe for containing a cryoprotectant and a pushing mechanism for displacing the syringe. The syringe pump is configured to discharge the cryoprotectant through the dispense tube and into the vessel, thereby dispensing the cryoprotectant into the sperm cell fluid. Mixing of the fluids is achieved using a shaker table agitation.
The disclosure relates to Bovine germplasm of Bos taurus variety HO840M003150607238. Included in the present disclosure are cells comprising the genome of Bovine variety HO840M003150607238 characterized by the presence of homozygous loci and spermatozoa obtained from said cells. Also provided by the present disclosure are tissue cultures of cells, animals obtained from said cells, and parts thereof, including F1 spermatozoa. The disclosure further provides for methods of breeding, selecting, and using the germplasm to improve existing commercial cattle herds generated from in vitro fertilization methods and progeny cattle obtained from in vitro fertilization and implantation and artificial insemination methods.
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
H01L 31/024 - Dispositions pour le refroidissement, le chauffage, la ventilation ou la compensation de température
H01L 31/107 - Dispositifs sensibles au rayonnement infrarouge, visible ou ultraviolet caractérisés par une seule barrière de potentiel ou de surface la barrière de potentiel fonctionnant en régime d'avalanche, p.ex. photodiode à avalanche
A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
F04B 19/00 - "Machines" ou pompes ayant des caractéristiques particulières non prévues dans les groupes ou présentant un intérêt autre que celui visé par ces groupes
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
G01N 15/10 - Recherche de particules individuelles
Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic device has at least one flow focusing channel where the components are focused or re-oriented by the geometry of the channel. From an upstream end of the flow focusing channel to a downstream end of the flow focusing channel, at least a portion of the flow focusing channel has a reduction in height and at least a portion of the flow focusing channel narrows in width, thereby geometrically constricting the flow focusing channel. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 15/06 - Recherche de la concentration des suspensions de particules
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
B65G 51/00 - Transport d'objets par tuyaux ou tubes utilisant l'écoulement ou la pression d'un fluideTransport d'objets sur une surface plane, p. ex. le fond d'un caniveau, par jets disposés le long de la surface
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
A microfluidic system configured to focus particles suspended in a fluid. One general aspect includes a microfluidic system comprising one or more substrates and a focusing channel formed in the one or more substrates and spanning a length from an inlet to an outlet for receiving a flow of particles suspended in fluid, wherein the particles have a diameter (a) and the focusing channel has a hydraulic diameter (dh).
A microfluidic system configured to focus particles suspended in a fluid. One general aspect includes a microfluidic system comprising one or more substrates and a focusing channel formed in the one or more substrates and spanning a length from an inlet to an outlet for receiving a flow of particles suspended in fluid, wherein the particles have a diameter (a) and the focusing channel has a hydraulic diameter (dh).
Applicants have identified that three critical phenotypic/genetic measures are highly correlated with transition period health and may be used in selection and breeding protocols and/or in combination with traditional breeding and marker assisted selection methods to improve predictability of transition period health. According to the invention genetic evaluations for mastitis, ketosis, and metritis have been found to be highly predictive of overall transition health. The genetic evaluations are produced by directly measuring thousands of clinical cases of mastitis, ketosis, and metritis in ancestors of a particular animal and using this data in selection. Applicant's selection criteria and quickly impact a breeders population by reducing transition cow disease incidence in the initial population and in progeny.
G16B 20/40 - Génétique de populationDéséquilibre de liaison
G16B 20/20 - Détection d’allèles ou de variantes, p. ex. détection de polymorphisme d’un seul nucléotide
G16H 50/30 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le calcul des indices de santéTIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour l’évaluation des risques pour la santé d’une personne
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
A61D 19/04 - Instruments ou procédés pour la reproduction ou la fertilisation pour la transplantation d'embryons
95.
Avalanche photodiode and cytometer sperm sex sensing apparatus
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
H01L 31/107 - Dispositifs sensibles au rayonnement infrarouge, visible ou ultraviolet caractérisés par une seule barrière de potentiel ou de surface la barrière de potentiel fonctionnant en régime d'avalanche, p.ex. photodiode à avalanche
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
H01L 31/024 - Dispositions pour le refroidissement, le chauffage, la ventilation ou la compensation de température
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
H01L 31/107 - Dispositifs sensibles au rayonnement infrarouge, visible ou ultraviolet caractérisés par une seule barrière de potentiel ou de surface la barrière de potentiel fonctionnant en régime d'avalanche, p.ex. photodiode à avalanche
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
H01L 31/024 - Dispositions pour le refroidissement, le chauffage, la ventilation ou la compensation de température
Technologies for assessing, quantifying and isolating sperm cell populations and/or subpopulations having specific genetic signatures are provided, as well as methods and systems to assess the efficacy of chromosomal differentiation processes. Compositions for identification and differentiation of X-chromosomes and Y-chromosomes in DNA are also provided.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6879 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détermination du sexe
