An improved method of deprotection in solid phase peptide synthesis is disclosed. In particular the deprotecting composition is added in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated acid from the preceding coupling cycle, and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle. Thereafter, the ambient pressure in the vessel is reduced with a vacuum pull to remove the deprotecting composition without any draining step and without otherwise adversely affecting the remaining materials in the vessel or causing problems in subsequent steps in the SPPS cycle.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
A process for deprotecting a protected amino acid during solid phase peptide synthesis (SPPS) includes removing a protecting group of a protected amino acid in a reaction vessel with a deprotecting base present in the reaction vessel in an amount greater than zero to about 5 vol%, based on the total volume (100 vol%) of a deprotection reaction mixture in the reaction vessel. At least a portion of the deprotecting base evaporates into an upper interior portion of the reaction vessel (e.g., the headspace) during the removing step. The process also includes directing an inert gas through the reaction vessel to remove evaporated deprotecting base from the interior of the reaction vessel (e.g., from the headspace) during the step of removing the protecting group.
A process for deprotecting a protected amino acid during solid phase peptide synthesis (SPPS) includes removing a protecting group of a protected amino acid in a reaction vessel with a deprotecting base present in the reaction vessel in an amount greater than zero to about 5 vol%, based on the total volume (100 vol%) of a deprotection reaction mixture in the reaction vessel. At least a portion of the deprotecting base evaporates into an upper interior portion of the reaction vessel (e.g., the headspace) during the removing step. The process also includes directing an inert gas through the reaction vessel to remove evaporated deprotecting base from the interior of the reaction vessel (e.g., from the headspace) during the step of removing the protecting group.
A process for deprotecting a protected amino acid during solid phase peptide synthesis (SPPS) includes removing a protecting group of a protected amino acid in a reaction vessel with a deprotecting base present in the reaction vessel in an amount greater than zero to about 5 vol %, based on the total volume (100 vol %) of a deprotection reaction mixture in the reaction vessel. At least a portion of the deprotecting base evaporates into an upper interior portion of the reaction vessel (e.g., the headspace) during the removing step. The process also includes directing an inert gas through the reaction vessel to remove evaporated deprotecting base from the interior of the reaction vessel (e.g., from the headspace) during the step of removing the protecting group.
In an acid digestion reaction performed in a vessel, an elastic lid or septum can be mounted to the mouth of the vessel with a flanged band. In response to determining that a predetermined pressure has been reached in the vessel, a closing force being applied to the lid or septum can be reduced to initiate venting from the vessel. One or more parameters, including the closing force, can be monitored during the venting, and the closing force can be responsively adjusted to optimize the venting. The closing force can be provided by a clamping apparatus that may or may not include a hydraulic system. The clamping apparatus can be configured to increase the closing force in response to increased hydraulic pressure in the hydraulic system. The clamping apparatus can include an engager having an upwardly extending recess extending at least partially around a downwardly protruding portion of the engager.
In an acid digestion reaction performed in a vessel, an elastic lid or septum can be mounted to the mouth of the vessel with a flanged band. In response to determining that a predetennined pressure has been reached in the vessel, a closing force being applied to the lid or septum can be reduced to initiate venting from the vessel. One or more parameters, including the closing force, can be monitored during the venting, and the closing force can be responsively adjusted to optimize the venting. The closing force can be provided by a clamping apparatus that may or may not include a hydraulic system. The clamping apparatus can be configured to increase the closing force in response to increased hydraulic pressure in the hydraulic system. The clamping apparatus can include an engager having an upwardly extending recess extending at least partially around a downwardly protruding portion of the engager.
B01J 19/24 - Réacteurs fixes sans élément interne mobile
B01J 3/03 - Récipients sous pression, ou récipients sous vide, comportant des organes de fermeture ou des joints d'étanchéité spécialement adaptés à cet effet
8.
SOLID PHASE PEPTIDE SYNTHESIS (SPPS) PROCESSES AND ASSOCIATED SYSTEMS
A process for deprotecting an amino acid during solid phase peptide synthesis includes removing a protecting group of a protected amino acid with a deprotecting agent in a reaction vessel, during which at least a portion of the deprotecting agent volatizes or evaporates into an upper interior portion of the reaction vessel. The process further includes directing an inert gas through the interior of the reaction vessel to purge volatized deprotecting agent from the interior of the reaction vessel. The present disclosure also relates to a solid phase peptide synthesis system for deprotecting an amino acid.
A process for deprotecting an amino acid during solid phase peptide synthesis includes removing a protecting group of a protected amino acid with a deprotecting agent in a reaction vessel, during which at least a portion of the deprotecting agent volatizes or evaporates into an upper interior portion of the reaction vessel. The process further includes directing an inert gas through the interior of the reaction vessel to purge volatized deprotecting agent from the interior of the reaction vessel. The present disclosure also relates to a solid phase peptide synthesis system for deprotecting an amino acid.
A structural improvement for microwave-assisted high temperature high-pressure chemistry vessel systems is disclosed that among other advantages offers dynamic venting and resealing while a reaction proceeds and eliminates the risk of cross contamination associated with systems that use a common pressurized chamber. The improvement includes a relatively thin-walled disposable liner cylinder that includes one closed end and one open end defining a mouth, and a liner cap positioned in the mouth of the rigid liner cylinder for closing the rigid liner cylinder. The liner cap includes a depending column that engages the inside diameter of the rigid liner cylinder, and a disk at one end of the depending column having a diameter sufficient to rest upon the rigid liner cylinder without falling into the rigid cylinder liner so that the cylindrical liner cap can rest in the rigid liner cylinder at the mouth of the rigid liner cylinder. The depending column, includes a passage to provide a gas venting space, and a dynamic venting action, between the liner cap and the rigid liner cylinder.
H05B 6/80 - Appareils pour applications spécifiques
H05B 6/68 - Circuits pour le contrôle ou la commande
B01J 3/04 - Récipients sous pression, p.ex. autoclaves
B01J 3/03 - Récipients sous pression, ou récipients sous vide, comportant des organes de fermeture ou des joints d'étanchéité spécialement adaptés à cet effet
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
11.
PRESSURE-RELEASE VESSEL WITH RIGID PROPORTIONAL LINER AND ASSOCIATED MICROWAVE-ASSISTED CHEMISTRY METHOD
A structural improvement for microwave-assisted high temperature high-pressure chemistry vessel systems including a relatively thin- walled disposable liner cylinder that includes one closed end and one open end defining a mouth, and a liner cap positioned in the mouth of the rigid liner cylinder for closing the rigid liner cylinder. The liner cap includes a depending column that engages the inside diameter of the rigid liner cylinder, and a disk at one end of the depending column having a diameter sufficient to rest upon the rigid liner cylinder without falling into the rigid cylinder liner so that the cylindrical liner cap can rest in the rigid liner cylinder at the mouth of the rigid liner cylinder. The depending column, includes a passage to provide a gas venting space, and a dynamic venting action, between the liner cap and the rigid liner cylinder.
B01J 3/03 - Récipients sous pression, ou récipients sous vide, comportant des organes de fermeture ou des joints d'étanchéité spécialement adaptés à cet effet
B01J 3/04 - Récipients sous pression, p.ex. autoclaves
B01J 19/02 - Appareils caractérisés par le fait qu'ils sont construits avec des matériaux choisis pour leurs propriétés de résistance aux agents chimiques
B01J 19/24 - Réacteurs fixes sans élément interne mobile
B01J 3/00 - Procédés utilisant une pression supérieure ou inférieure à la pression atmosphérique pour obtenir des modifications chimiques ou physiques de la matière; Appareils à cet effet
B65D 51/16 - Fermetures non prévues ailleurs avec moyens pour laisser partir l'air ou le gaz
A solvent system for solid phase peptide synthesis is disclosed that combines a morpholine-based compound and an alkoxybenzene-based compound to form a solvent that can be compatible with both polystyrene and/or PEG resins and that can produce purity yields at least comparable to conventional solvents such as DMF, DMA, and/or NMP.
A solvent system for solid phase peptide synthesis is disclosed that combines a morpholine-based compound and an alkoxybenzene-based compound to form a solvent that can be compatible with both polystyrene and/or PEG resins and that can produce purity yields at least comparable to conventional solvents such as DMF, DMA, and/or NMP.
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
C40B 50/14 - Synthèse en phase solide, c. à d. dans laquelle au moins un bloc servant à créer la bibliothèque est lié à un support solide au cours de la création de la bibliothèque; Procédés particuliers de clivage à partir du support solide
A volatile content analysis instrument is disclosed that includes a cavity and a microwave source positioned to produce and direct microwaves into the cavity at frequencies other than infrared frequencies. A balance is included with at least the balance pan (or platform) in the cavity. An infrared source is positioned to produce and direct infrared radiation into the cavity at frequencies other than the microwave frequencies produced by the microwave source. A lens is positioned between the infrared source and the balance pan for more efficiently directing infrared radiation to a sample on the balance pan. The lens has dimensions that preclude microwaves of the frequencies produced by the source and directed into the cavity from leaving the cavity.
G01N 5/04 - Analyse des matériaux par pesage, p.ex. pesage des fines particules séparées d'un gaz ou d'un liquide en éliminant un constituant, p.ex. par évaporation, et en pesant le reste
An extraction method for preparing samples for analytical analysis is disclosed. The method includes the steps of placing a sample matrix containing one or more analytes in a heat conductive sample cup, positioning the heat conductive sample cup in a pressure-resistant reaction chamber, dispensing solvent into the heat conductive sample cup, dispersing the solvent and the sample matrix in the sample cup in the reaction chamber, heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure, and releasing the extraction solvent extract from the sample cup at atmospheric pressure.
An instrument for extraction based molecular sample preparation and related processes is disclosed. The instrument includes a thermally conductive pressure resistant heating chamber and a thermally conductive sample cup positioned in the thermally conductive pressure resistant healing chamber for heating liquids and solids together in the thermally conductive sample cup. A liquid delivery inlet fixture in the thermally conductive pressure resistant heating chamber delivers liquids (solvent) from a supply to the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber, and a chiller in liquid communication with the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber receives heated liquids from the thermally conductive pressure resistant heating chamber when the chamber is opened to atmospheric pressure.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
B01L 7/00 - Appareils de chauffage ou de refroidissement; Dispositifs d'isolation thermique
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
B01L 9/06 - Supports de tubes à essai; Porte-tubes à essai
19.
Rapid energized dispersive solid phase extraction (SPE) for analytical analysis
An energized dispersive extraction method for sample preparation for analysis is disclosed. The method includes the steps of placing an extraction solvent, sorbent particles, and a sample matrix containing an analyte in a heat conductive sample cup; positioning the sample cup in a pressure-resistant reaction chamber; dispersing the solvent and the sample matrix in the sample cup in the reaction chamber; heating the sample matrix and the solvent in the sample cup in the reaction chamber to a temperature that generates an above-atmospheric pressure; draining the solvent extract from the sample cup at atmospheric pressure; and collecting the solvent extract for analysis.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific instruments for performing solvent extraction, namely, instruments for performing liquid-liquid extraction or solid-phase extraction (SPE); Instruments for performing solvent extraction of sequential samples; Instruments for performing solvent extraction using combinations of elevated temperature, elevated pressure, and ultrasonic agitation.
An instrument for extraction based molecular sample preparation and related processes is disclosed. The instrument includes a thermally conductive pressure resistant heating chamber and a thermally conductive sample cup positioned in the thermally conductive pressure resistant heating chamber for heating liquids and solids together in the thermally conductive sample cup. A liquid delivery inlet fixture in the thermally conductive pressure resistant heating chamber delivers liquids (solvent) from a supply to the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber, and a chiller in liquid communication with the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber receives heated liquids from the thermally conductive pressure resistant heating chamber when the chamber is opened to atmospheric pressure.
An energized dispersive extraction method for sample preparation for analysis is disclosed. The method includes the steps of placing an extraction solvent, sorbent particles, and a sample matrix containing an analyte in a heat conductive sample cup; positioning the sample cup in a pressure-resistant reaction chamber; dispersing the solvent and the sample matrix in the sample cup in the reaction chamber; heating the sample matrix and the solvent in the sample cup in the reaction chamber to a temperature that generates an above-atmospheric pressure; draining the solvent extract from the sample cup at atmospheric pressure; and collecting the solvent extract for analysis.
An extraction method for preparing samples for analytical analysis is disclosed. The method includes the steps of placing a sample matrix containing one or more analytes in a heat conductive sample cup, positioning the heat conductive sample cup in a pressure-resistant reaction chamber, dispensing solvent into the heat conductive sample cup, dispersing the solvent and the sample matrix in the sample cup in the reaction chamber, heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure, and releasing the extraction solvent extract from the sample cup at atmospheric pressure.
A venting cap is disclosed for pressure vessels for microwave-assisted chemistry. The venting cap includes a flexible circular cover for closing the mouth of a reaction vessel, a flexible annular wall depending from the circular cover, and a flexible annular ring at the bottom of the annular wall and parallel to the circular cover for positioning the cap on a reaction vessel. At least one indentation in the circular cover minimizes distortion when any contents of a reaction vessel exert pressure against the cap, and at least one opening in the annular wall provides a ventilation path through the cap when gas pressure in a reaction vessel flexes the cap sufficiently to partially disengage at least a portion of the cap from the mouth of the reaction vessel.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
A vessel system for high-pressure reactions is disclosed. The system includes a plugged polymer cylinder reaction vessel with a pressure vent opening extending radially through the wall of the reaction vessel and a supporting frame into which the vessel is received. Complementing keying structure elements on the vessel and on the frame limit the orientation of the reaction vessel in the supporting frame and the radially extending vent opening to a defined single position.
B01J 3/03 - Récipients sous pression, ou récipients sous vide, comportant des organes de fermeture ou des joints d'étanchéité spécialement adaptés à cet effet
B01J 3/04 - Récipients sous pression, p.ex. autoclaves
B01J 19/02 - Appareils caractérisés par le fait qu'ils sont construits avec des matériaux choisis pour leurs propriétés de résistance aux agents chimiques
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
B01J 19/24 - Réacteurs fixes sans élément interne mobile
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Chemicals for solvent extraction, namely, sorbents for solvent extraction, sorbents for dispersive solid phase extraction; Extraction salts for solvent extraction and extraction salts for dispersive solid phase extraction
27.
IN-SITU SOLVENT RECYCLING PROCESS FOR SOLID PHASE PEPTIDE SYNTHESIS AT ELEVATED TEMPERATURES
An improvement in of deprotection in solid phase peptide synthesis is disclosed. The method includes the steps of adding the deprotection composition in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated amino acid from the preceding coupling cycle; and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle; and with the coupling solution at a temperature of at least 30°C.
An improvement in of deprotection in solid phase peptide synthesis is disclosed. The method includes the steps of adding the deprotection composition in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated amino acid from the preceding coupling cycle; and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle; and with the coupling solution at a temperature of at least 30°C.
An improved method of coupling amino acids into peptides or peptidomimetics is disclosed in which the activation and coupling are carried out in the same vessel, in the presence of a carbodiimide in an amount greater than 1 equivalent as compared to the amino acid, in the presence of an activator additive, and at a temperature greater than 30°C.
C07K 1/08 - Procédés généraux de préparation de peptides utilisant des groupes protecteurs ou des agents d'activation utilisant des agents d'activation
C07K 1/10 - Procédés généraux de préparation de peptides utilisant des agents de couplage
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific apparatus for chemical synthesis and chemical analysis, namely, instruments for precision volume control in microwave assisted chemical synthesis and related software sold as a unit
31.
In-situ solvent recycling process for solid phase peptide synthesis at elevated temperatures
An improvement of deprotection in solid phase peptide synthesis is disclosed. The method includes the steps of adding the deprotection composition in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated amino acid from the preceding coupling cycle; and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle; and with the coupling solution at a temperature of at least 30° C.
An improved method for coupling amino acids into peptides or peptidomimetics is disclosed that includes the steps of combining an amino acid, a carbodiimide, an activator additive, and a base at less than 1 equivalent compared to the amino acid to be activated; and carrying out the activation and coupling at a temperature greater than 30° C.
C07K 1/00 - Procédés généraux de préparation de peptides
C07K 1/08 - Procédés généraux de préparation de peptides utilisant des groupes protecteurs ou des agents d'activation utilisant des agents d'activation
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
A vessel system for high-pressure reactions is disclosed. The system includes a plugged polymer cylinder reaction vessel with a pressure vent opening extending radially through the wall of the reaction vessel and a supporting frame into which the vessel is received. Complementing keying structure elements on the vessel and on the frame limit the orientation of the reaction vessel in the supporting frame and the radially extending vent opening to a defined single position.
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en général; Appareils appropriés
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
34.
HIGH TEMPERATURE PRESSURE DIGESTION VESSEL SYSTEM WITH DUAL ACTION SEAL
A vessel system for high-pressure reactions is disclosed. The system includes a plugged polymer cylinder reaction vessel with a pressure vent opening extending radially through the wall of the reaction vessel and a supporting frame into which the vessel is received. Complementing keying structure elements on the vessel and on the frame limit the orientation of the reaction vessel in the supporting frame and the radially extending vent opening to a defined single position.
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en général; Appareils appropriés
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
An instrument system for acid digestion is disclosed. The instrument includes a heating block, a reaction vessel formed of a polymer that is resistant to acid and other chemical attack at temperatures above 150° C. and that has a structure (thickness, etc.) sufficient to withstand pressures above atmospheric, a metal sleeve surrounding the polymeric reaction vessel, and an opening in the block that has a cross-section corresponding to the cross-section of the metal sleeve.
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en général; Appareils appropriés
B01J 3/03 - Récipients sous pression, ou récipients sous vide, comportant des organes de fermeture ou des joints d'étanchéité spécialement adaptés à cet effet
B01J 3/00 - Procédés utilisant une pression supérieure ou inférieure à la pression atmosphérique pour obtenir des modifications chimiques ou physiques de la matière; Appareils à cet effet
A vessel system for high-pressure reactions is disclosed. The system includes a plugged polymer cylinder reaction vessel with a pressure vent opening extending radially through the wall of the reaction vessel and a supporting frame into which the vessel is received. Complementing keying structure elements on the vessel and on the frame limit the orientation of the reaction vessel in the supporting frame and the radially extending vent opening to a defined single position.
B01J 19/02 - Appareils caractérisés par le fait qu'ils sont construits avec des matériaux choisis pour leurs propriétés de résistance aux agents chimiques
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
B01J 19/24 - Réacteurs fixes sans élément interne mobile
An improved method of deprotection in solid phase peptide synthesis is disclosed. In particular the deprotecting composition is added in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated acid from the preceding coupling cycle, and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle. Thereafter, the ambient pressure in the vessel is reduced with a vacuum pull to remove the deprotecting composition without any draining step and without otherwise adversely affecting the remaining materials in the vessel or causing problems in subsequent steps in the SPPS cycle.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
C07K 1/107 - Procédés généraux de préparation de peptides par modification chimique de peptides précurseurs
C07K 14/005 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de virus
C12N 7/00 - Virus, p.ex. bactériophages; Compositions les contenant; Leur préparation ou purification
C07K 14/47 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
C07K 7/08 - Peptides linéaires ne contenant que des liaisons peptidiques normales ayant de 12 à 20 amino-acides
C07K 14/46 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant d'humains provenant de vertébrés
An improved method of deprotection in solid phase peptide synthesis is disclosed. In particular the deprotecting composition is added in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated acid from the preceding coupling cycle, and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle. Thereafter, the ambient pressure in the vessel is reduced with a vacuum pull to remove the deprotecting composition without any draining step and without otherwise adversely affecting the remaining materials in the vessel or causing problems in subsequent steps in the SPPS cycle.
An improved method of deprotection in solid phase peptide synthesis is disclosed. In particular the deprotecting composition is added in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated acid from the preceding coupling cycle, and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle. Thereafter, the ambient pressure in the vessel is reduced with a vacuum pull to remove the deprotecting composition without any draining step and without otherwise adversely affecting the remaining materials in the vessel or causing problems in subsequent steps in the SPPS cycle.
An instrument and method for high pressure microwave assisted chemistry are disclosed. The method includes the steps of applying microwave radiation to a sample in a sealed vessel while measuring the temperature of the sample and measuring the pressure generated inside the vessel and until the measured pressure reaches a designated set point, opening the vessel to release gases until the pressure inside the vessel reaches a lower designated set point, closing the vessel, and repeating the steps of opening the vessel at designated pressure set points and closing the vessel at designated pressure set points to the sample until the sample reaction reaches a designated high temperature. The designated set points can controllably differ from one another as the reaction proceeds. Microwave energy can be applied continuously or intermittently during the opening and closing steps. The apparatus includes a microwave cavity, a microwave transparent pressure resistant reaction vessel in the cavity, a cap on the reaction vessel, a pressure sensor for measuring pressure in the vessel, a temperature sensor, and means for opening and closing the cap at predetermined pressure set points measured by the pressure sensor to release pressure from the vessel.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
G01N 1/44 - Traitement d'échantillons mettant en œuvre un rayonnement, p.ex. de la chaleur
H05B 6/80 - Appareils pour applications spécifiques
A precision volumetric liquid dispensing instrument is disclosed that includes two pressure sensors and a fluid passageway with a defined volume portion in communication with the two sensors for receiving and distributing liquid in relatively small volumes. One of the pressure sensors is positioned to measure pressure at one portion of the defined volume portion of the fluid passageway and the other of the gas pressure sensors is positioned to measure gas pressure at a different portion of the defined volume portion of the passageway. At least one valve is in communication with the passageway for moving fluids into or out of the defined volume portion of the fluid passageway, and a processor carries out a step selected from the group consisting of (i) calculating the volume of the liquid based upon the measured pressure and (ii) metering a liquid into the defined volume portion of the fluid passageway until the measured pressure indicates that a desired volume of fluid is in the fluid passageway.
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
G05D 7/06 - Commande de débits caractérisée par l'utilisation de moyens électriques
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en général; Appareils appropriés
G01F 11/28 - Appareils qu'il faut actionner de l'extérieur, adaptés à chaque opération répétée et identique, pour mesurer et séparer le volume prédéterminé d'un fluide ou d'un matériau solide fluent à partir d'une alimentation ou d'un récipient sans tenir compte avec des chambres de mesure fixes ayant un volume constant au cours du mesurage
F04F 5/24 - Pompes à jet, p.ex. dispositifs dans lesquels le flux est produit par la chute de pression causée par la vitesse d'un autre fluide le fluide inducteur étant un fluide compressible déplaçant des liquides, p.ex. contenant des solides ou bien déplaçant des liquides et des fluides compressibles
B01J 4/00 - Dispositifs d'alimentation; Dispositifs de commande d'alimentation ou d'évacuation
B01J 4/02 - Dispositifs d'alimentation; Dispositifs de commande d'alimentation ou d'évacuation pour introduire des quantités mesurées de réactifs
A volatile content analysis instrument is disclosed that includes a cavity and a microwave source positioned to produce and direct microwaves into the cavity at frequencies other than infrared frequencies. A balance is included with at least the balance pan (or platform) in the cavity. An infrared source is positioned to produce and direct infrared radiation into the cavity at frequencies other than the microwave frequencies produced by the microwave source. A lens is positioned between the infrared source and the balance pan for more efficiently directing infrared radiation to a sample on the balance pan. The lens has dimensions that preclude microwaves of the frequencies produced by the source and directed into the cavity from leaving the cavity.
G01N 5/04 - Analyse des matériaux par pesage, p.ex. pesage des fines particules séparées d'un gaz ou d'un liquide en éliminant un constituant, p.ex. par évaporation, et en pesant le reste
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific instruments for performing solvent extraction, namely, instruments for performing liquid-liquid extraction or solid-phase extraction (SPE); Instruments for performing solvent extraction of sequential samples; Instruments for performing solvent extraction using combinations of elevated temperature, elevated pressure, and ultrasonic agitation
An improved method for coupling carboxylic acids and amines is disclosed that includes the steps of combining a hyper-acid sensitive linker connecting an amine and a resin, a carboxylic acid, a carbodiimide, an activator additive, and a base, and carrying out the activation and coupling at a temperature greater than 30° C.
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
C07K 1/08 - Procédés généraux de préparation de peptides utilisant des groupes protecteurs ou des agents d'activation utilisant des agents d'activation
49.
IMPROVED COUPLING METHOD FOR PEPTIDE SYNTHESIS AT ELEVATED TEMPERATURES
An improved method for coupling carboxylic acids and amines is disclosed that includes the steps of combining a hyper-acid sensitive linker connecting an amine and a resin, a carboxylic acid, a carbodiimide, an activator additive, and a base, and carrying out the activation and coupling at a temperature greater than 30°C.
C07C 231/02 - Préparation d'amides d'acides carboxyliques à partir d'acides carboxyliques ou à partir de leurs esters, anhydrides ou halogénures par réaction avec de l'ammoniac ou des amines
A method of element testing is disclosed that includes the steps of weighing a sample, pressing the sample between pads, applying microwave radiation to the sample and pads in the presence of a microwave susceptor material and ashing the pressed sample, adding the pads and the ashed sample to an acid, and forwarding filtrate from the pad-sample-acid mixture to a spectrometer.
G01N 21/31 - Couleur; Propriétés spectrales, c. à d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p.ex. spectrométrie d'absorption atomique
G01N 21/72 - Systèmes dans lesquels le matériau analysé est excité de façon à ce qu'il émette de la lumière ou qu'il produise un changement de la longueur d'onde de la lumière incidente excité thermiquement en utilisant des brûleurs à flamme
G01N 21/73 - Systèmes dans lesquels le matériau analysé est excité de façon à ce qu'il émette de la lumière ou qu'il produise un changement de la longueur d'onde de la lumière incidente excité thermiquement en utilisant des brûleurs ou torches à plasma
An instrument and method for high pressure microwave assisted chemistry are disclosed. The method includes the steps of applying microwave radiation to a sample in a sealed vessel while measuring the temperature of the sample and measuring the pressure generated inside the vessel and until the measured pressure reaches a designated set point, opening the vessel to release gases until the pressure inside the vessel reaches a lower designated set point, closing the vessel, and repeating the steps of opening the vessel at designated pressure set points and closing the vessel at designated pressure set points to the sample until the sample reaction reaches a designated high temperature. The designated set points can controllably differ from one another as the reaction proceeds. Microwave energy can be applied continuously or intermittently during the opening and closing steps. The apparatus includes a microwave cavity, a microwave transparent pressure resistant reaction vessel in the cavity, a cap on the reaction vessel, a pressure sensor for measuring pressure in the vessel, a temperature sensor, and means for opening and closing the cap at predetermined pressure set points measured by the pressure sensor to release pressure from the vessel.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
H05B 6/80 - Appareils pour applications spécifiques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Microwave based instruments for testing samples and parts thereof; microwave based instruments for chemical synthesis and parts thereof; microwave digestion vessels; controlled pressure release vessels for microwave assisted chemistry; digestion vessels for microwave assisted chemical processes; digestion vessels for use at high pressures; digestion vessels for use at high temperatures; parts for microwave digestion vessels.
53.
Instrument for performing microwave-assisted reactions
An instrument for performing microwave-assisted reactions and an associated method are disclosed. The instrument typically includes (i) a microwave-radiation source, (ii) a cavity, (iii) a waveguide in microwave communication with the microwave-radiation source and the cavity, (iv) at least one reaction-vessel sensor for determining the number and/or type of reaction vessels positioned within the cavity, (v) an interface, and (vi) a computer controller. The computer controller is typically in communication with the interface, the microwave-radiation source, and the reaction-vessel sensor. The computer controller is typically capable of determining the output of the microwave-radiation source in response to the number and/or type of reaction vessels positioned within the cavity.
09 - Appareils et instruments scientifiques et électriques
Produits et services
instruments for scientific research in laboratories, namely, vessels; vessels for high temperature and high pressure microwave digestion for laboratory use; vessels for peptide synthesis and extraction for laboratory use
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemicals used in science and chemical substances, namely, chemicals for use in industry and science, and chemical reagents for non-medical purposes Educational services, namely, providing live and on-line seminars in the field of science; Training of specialists in the microwave-assisted chemistry industry; Training services in the field of governmental compliance for the pharmaceutical and biotechnology industries [ Providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto ]
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific instruments for performing microwave-assisted chemical reactions and that incorporate hardware and software for providing access between the instruments and the Internet including instruments that incorporate Ethernet or wireless local area broadband connection standards and capabilities; instruments for performing microwave-assisted chemical reactions that include short range radio frequency communication between the instrument and other devices; instruments for performing microwave-assisted chemical reactions that include short-range wireless interconnection communication capabilities; instruments for performing microwave-assisted chemical reactions that exchange data with remote or mobile devices; instruments for performing microwave-assisted chemical reactions that can be controlled from remote or mobile devices
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific instruments and vessels for performing microwave-assisted chemical reactions in the vessels in the instruments, and in which the instruments include infrared temperature sensors operating at frequencies to which the reaction vessel materials are transparent to provide non-contact temperature measurement for reactions in the vessels in the instruments
58.
Control apparatus for dispensing small precise amounts of liquid reagents
A precision volumetric liquid dispensing instrument is disclosed that includes two pressure sensors and a fluid passageway with a defined volume portion in communication with the two sensors for receiving and distributing liquid in relatively small volumes. One of the pressure sensors is positioned to measure pressure at one portion of the defined volume portion of the fluid passageway and the other of the gas pressure sensors is positioned to measure gas pressure at a different portion of the defined volume portion of the passageway. At least one valve is in communication with the passageway for moving fluids into or out of the defined volume portion of the fluid passageway, and a processor carries out a step selected from the group consisting of (i) calculating the volume of the liquid based upon the measured pressure and (ii) metering a liquid into the defined volume portion of the fluid passageway until the measured pressure indicates that a desired volume of fluid is in the fluid passageway.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
G05D 7/06 - Commande de débits caractérisée par l'utilisation de moyens électriques
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en général; Appareils appropriés
G01F 11/28 - Appareils qu'il faut actionner de l'extérieur, adaptés à chaque opération répétée et identique, pour mesurer et séparer le volume prédéterminé d'un fluide ou d'un matériau solide fluent à partir d'une alimentation ou d'un récipient sans tenir compte avec des chambres de mesure fixes ayant un volume constant au cours du mesurage
F04F 5/24 - Pompes à jet, p.ex. dispositifs dans lesquels le flux est produit par la chute de pression causée par la vitesse d'un autre fluide le fluide inducteur étant un fluide compressible déplaçant des liquides, p.ex. contenant des solides ou bien déplaçant des liquides et des fluides compressibles
B01J 4/00 - Dispositifs d'alimentation; Dispositifs de commande d'alimentation ou d'évacuation
B01J 4/02 - Dispositifs d'alimentation; Dispositifs de commande d'alimentation ou d'évacuation pour introduire des quantités mesurées de réactifs
A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.
G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
G01N 1/00 - Echantillonnage; Préparation des éprouvettes pour la recherche
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 21/25 - Couleur; Propriétés spectrales, c. à d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G01N 1/28 - Préparation d'échantillons pour l'analyse
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
A method of measuring NMR response in an NMR instrument includes heating a sample at a heater temperature that is higher than the temperature of the interior of the NMR instrument, positioning the heated sample in the NMR instrument, and measuring the NMR response of the heat sample. Typically, the sample is dry and includes fat. Furthermore, a method of determining an amount of a component of a sample includes positioning a sample in an NMR instrument, applying a sequence of radio-frequency pulses to the sample, measuring the amplitudes of the signals produced by the application of the sequence of radio-frequency pulses, and determining the amount of a component in the sample using the measured amplitudes of the signals. The disclosed methods typically provide accurate analysis of samples in a shorter time period than traditional NMR techniques and solvent-based analysis techniques.
G01N 24/08 - Recherche ou analyse des matériaux par l'utilisation de la résonance magnétique nucléaire, de la résonance paramagnétique électronique ou d'autres effets de spin en utilisant la résonance magnétique nucléaire
A method of measuring NMR response in an NMR instrument includes heating a sample at a heater temperature that is higher than the temperature of the interior of the NMR instrument, positioning the heated sample in the NMR instrument, and measuring the NMR response of the heat sample. Typically, the sample is dry and includes fat. Furthermore, a method of determining an amount of a component of a sample includes positioning a sample in an NMR instrument, applying a sequence of radio-frequency pulses to the sample, measuring the amplitudes of the signals produced by the application of the sequence of radio-frequency pulses, and determining the amount of a component in the sample using the measured amplitudes of the signals. The disclosed methods typically provide accurate analysis of samples in a shorter time period than traditional NMR techniques and solvent-based analysis techniques.
G01N 24/08 - Recherche ou analyse des matériaux par l'utilisation de la résonance magnétique nucléaire, de la résonance paramagnétique électronique ou d'autres effets de spin en utilisant la résonance magnétique nucléaire
G01R 33/44 - Dispositions ou appareils pour la mesure des grandeurs magnétiques faisant intervenir la résonance magnétique utilisant la résonance magnétique nucléaire [RMN]
A solid phase peptide synthesis method is disclosed. The method includes the steps of deprotecting an amino group in its protected form that is protected with a protecting group that includes an α,β-unsaturated sulfone; washing the deprotected acid in a solvent selected from the group consisting of water, alcohol, and mixtures of water and alcohol; coupling the deprotected acid to a resin-based peptide or a resin-based amino acid; and washing the coupled composition in a solvent selected from the group consisting of water, alcohol, and mixtures of water and alcohol.
An instrument for performing microwave-assisted reactions typically includes (i) a microwave-radiation source, (ii) a cavity, (iii) a waveguide in microwave communication with the microwave-radiation source and the cavity, (iv) at least one reaction-vessel sensor for determining the number and/or type of reaction vessels positioned within the cavity, (v) an interface, and (vi) a computer controller. The computer controller is typically in communication with the interface, the microwave-radiation source, and the reaction-vessel sensor. The computer controller is typically capable of determining the output of the microwave-radiation source in response to the number and/or type of reaction vessels positioned within the cavity.
A solid phase peptide synthesis method is disclosed. The method includes the steps of deprotecting an amino group in its protected form that is protected with a protecting group containing a Michael acceptor site composed of an α,β-unsaturated sulfone in a solvent selected from the group consisting of water, alcohol, and mixtures of water and alcohol; washing the deprotected acid in a solvent selected from the group consisting of water, alcohol, and mixtures of water and alcohol; coupling the deprotected acid to a resin-based peptide or a resin-based amino acid in a solvent selected from the group consisting of water, alcohol, and mixtures of water and alcohol; and washing the coupled composition in a solvent selected from the group consisting of water, alcohol, and mixtures of water and alcohol.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific apparatus and instruments; laboratory apparatus and instruments [other than for medical use]; pressure measuring, pressure monitoring and pressure control apparatus and instruments; microwave synthesis apparatus; apparatus and instruments for use with microwave synthesis apparatus; apparatus and instruments for microwave assisted synthesis; apparatus for releasing gases at selected pressures and temperatures.
An instrument and method for high pressure microwave assisted chemistry are disclosed. The method includes the steps of applying microwave radiation to a sample in a sealed vessel while measuring the temperature of the sample and measuring the pressure generated inside the vessel and until the measured pressure reaches a designated set point, opening the vessel to release gases until the pressure inside the vessel reaches a lower designated set point, closing the vessel, and repeating the steps of opening the vessel at designated pressure set points and closing the vessel at designated pressure set points to the sample until the sample reaction reaches a designated high temperature. The designated set points can controllably differ from one another as the reaction proceeds. Microwave energy can be applied continuously or intermittently during the opening and closing steps. The apparatus includes a microwave cavity, a microwave transparent pressure resistant reaction vessel in the cavity, a cap on the reaction vessel, a pressure sensor for measuring pressure in the vessel, a temperature sensor, and means for opening and closing the cap at predetermined pressure set points measured by the pressure sensor to release pressure from the vessel.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
71.
High temperature high pressure vessel for microwave assisted chemistry
A method of conducting microwave-assisted high pressure high temperature chemistry is disclosed. The method includes the steps of digesting a sample in a strong acid at a temperature of at least 200° C. in a pressure resistant vessel that includes a lid while exerting a defined force against the lid in order to maintain gases under pressure in the vessel; directing gas under excess pressure from the vessel into a circumferential passage defined by the vessel and its pressure resistant lid; and directing the gas from the circumferential passage outwardly from the lid while preventing gas from flowing outwardly over the edge of the vessel.
G01N 1/00 - Echantillonnage; Préparation des éprouvettes pour la recherche
B01J 3/03 - Récipients sous pression, ou récipients sous vide, comportant des organes de fermeture ou des joints d'étanchéité spécialement adaptés à cet effet
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en général; Appareils appropriés
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
H05B 6/80 - Appareils pour applications spécifiques
A protein analysis kit is disclosed. The kit includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup.
A vessel assembly is disclosed for high pressure high temperature chemistry. The vessel assembly includes a cylindrical vessel and a cylindrical vessel seal cover. The vessel and the seal cover have respective surfaces that, when engaged, define a circumferential interior passage between the vessel and the seal cover. A pressure release opening in the seal cover is in fluid communication with the circumferential passage. A retaining ring surrounds the vessel and the seal cover at the position where the vessel and the seal cover meet to maintain an outer circumferential engagement between the seal cover and the vessel when pressure forces gases in the vessel to flow into the circumferential passage and then outwardly from the pressure release opening.
B01J 3/00 - Procédés utilisant une pression supérieure ou inférieure à la pression atmosphérique pour obtenir des modifications chimiques ou physiques de la matière; Appareils à cet effet
74.
HIGH TEMPERATURE HIGH PRESSURE VESSEL FOR MICROWAVE ASSISTED CHEMISTRY
A vessel assembly is disclosed for high pressure high temperature chemistry. The vessel assembly includes a cylindrical vessel and a cylindrical vessel seal cover. The vessel and the seal cover have respective surfaces that, when engaged, define a circumferential interior passage between the vessel and the seal cover. A pressure release opening in the seal cover is in fluid communication with the circumferential passage. A retaining ring surrounds the vessel and the seal cover at the position where the vessel and the seal cover meet to maintain an outer circumferential engagement between the seal cover and the vessel when pressure forces gases in the vessel to flow into the circumferential passage and then outwardly from the pressure release opening.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
B01J 19/02 - Appareils caractérisés par le fait qu'ils sont construits avec des matériaux choisis pour leurs propriétés de résistance aux agents chimiques
B65D 85/00 - Réceptacles, éléments d'emballage ou paquets spécialement adaptés à des objets ou à des matériaux particuliers
An instrument and associated method are disclosed for the loss-on-drying determination of the volatile content of a wide variety of samples. The instrument includes a cavity in which a sample for which the volatile content is to be determined can be placed, a first source for introducing microwaves into the cavity that have frequencies substantially other than infrared frequencies, a second source for introducing radiant heat into the cavity at frequencies different from the frequencies introduced by the first source, an analytical balance for measuring the weight of a sample while the sample is in the cavity and on the balance, a temperature sensor capable of measuring and positioned to measure the temperature of a sample in the cavity and on the balance, and a processor in communication with the temperature sensor and each of the first and second sources for controlling the introduction of the frequencies of microwave and radiant energy into the cavity in response to the temperatures measured by the temperature sensor to control the sample temperature until the microwaves from the first source and the radiant heat from the second source dry the sample sufficiently for the processor to determine the volatile content of the sample based on the weight change of the sample on the balance.
A protein analysis instrument and method are disclosed. The method includes mixing a binding dye composition and a protein sample using a homogenizer, measuring a parameter selected from the group consisting of the speed of the homogenizer and the resistance of the mixture to the homogenizer, adjusting the speed of the homogenizer based upon the measured parameter, pumping unreacted dye composition from the mixture and to a calorimeter, and measuring the absorbance of the dye composition in the calorimeter. Aspects of the invention also include inserting a spout into a sample cup at a position where the spout opening is positioned to avoid foam and precipitate generated by the mixing step and above the bottom of the sample cup and thereafter pumping the dye composition from the mixture in the sample cup through the spout and to a colorimeter.
G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c. à d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p.ex. utilisation de bandes de papier indicateur
A direct rapid automated protein analyzer is disclosed. The protein analyzer includes means for reducing protein samples to small particles, a reaction vessel in material transfer communication with the homogenizer, a reservoir for binding dye composition in fluid communication with the reaction vessel, a metering pump in fluid communication with the reservoir and the reaction vessel for distributing discrete predetermined amounts of a binding dye composition to the reaction vessel, a filter in fluid communication with the reaction vessel for separating solids from filtrate after a dye binding reaction has taken place in the reaction vessel, and a calorimeter in fluid communication with the filter and the reaction vessel for measuring the absorbance of the filtrate from the reaction vessel and the filter. The rapid analyzer can be used in conjunction with a kit that includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup. The kit can also include dye concentrate solution.
G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
A method of calibrating a colorimeter-based protein analyzer is disclosed. The method includes the steps of measuring the absorbance of a protein-binding reference dye of known starting concentration on a protein analyzer that includes a colorimeter on at least two protein-containing samples having known protein content; plotting the absorbance of each of the known samples against the known protein content as a straight line that includes a slope and intercept; measuring the absorbance on a protein sample of the same type of protein having an unknown protein content in the same instrument; and determining the protein content of the unknown sample based upon the measured absorbance and the slope and intercept from the known samples. A method of calibrating a plurality of colorimeter-based protein analyzing instruments to produce accurate and precise results on any one or more of a plurality of types of protein is also disclosed.
G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p.ex. utilisation de bandes de papier indicateur
G01D 18/00 - Test ou étalonnage des appareils ou des dispositions prévus dans les groupes
An instrument and associated method are disclosed for preparing samples for column chromatography. The method includes the steps of applying microwave energy to a sample composition containing at least one solvent to encourage a chemical reaction and generate desired products, thereafter mixing an absorbent media with the sample to absorb the solvent, the media being compatible with liquid chromatography that will separate the expected products, being chemically inert to the expected products, and being added in an amount sufficient to provide a substantially dry mixture of the media and the sample, but less than an amount that overly broadens the resolution of the sample during liquid chromatography, thereafter applying microwave energy to the dry mixture of the media and the sample to thereby encourage the solvent to evaporate under the influence of the microwave energy, and thereafter adding the dry mixture of the media and the remaining sample to a liquid chromatography column and separating the components of the remaining sample for identification and purification purposes.
An instrument and associated method are disclosed for the loss-on-drying determination of the volatile content of a wide variety of samples. The instrument includes a cavity in which a sample for which the volatile content is to be determined can be placed, a first source for introducing microwaves into the cavity that have frequencies substantially other than infrared frequencies, a second source for introducing radiant heat into the cavity at frequencies different from the frequencies introduced by the first source, an analytical balance for measuring the weight of a sample while the sample is in the cavity and on the balance, a temperature sensor capable of measuring and positioned to measure the temperature of a sample in the cavity and on the balance, and a processor in communication with the temperature sensor and each of the first and second sources for controlling the introduction of the frequencies of microwave and radiant energy into the cavity in response to the temperatures measured by the temperature sensor to control the sample temperature until the microwaves from the first source and the radiant heat from the second source dry the sample sufficiently for the processor to determine the volatile content of the sample based on the weight change of the sample on the balance.
G01N 5/02 - Analyse des matériaux par pesage, p.ex. pesage des fines particules séparées d'un gaz ou d'un liquide en absorbant ou adsorbant les constituants d'un matériau et en déterminant la variation de poids de l'adsorbant, p.ex. en déterminant la teneur en eau
G01N 25/56 - Recherche ou analyse des matériaux par l'utilisation de moyens thermiques en recherchant la teneur en eau
G01J 5/00 - Pyrométrie des radiations, p.ex. thermométrie infrarouge ou optique
An instrument and associated method are disclosed for the loss-on-drying determination of the volatile content of a wide variety of samples. The instrument includes a cavity in which a sample for which the volatile content is to be determined can be placed, a first source for introducing microwaves into the cavity that have frequencies substantially other than infrared frequencies, a second source for introducing radiant heat into the cavity at frequencies different from the frequencies introduced by the first source, an analytical balance for measuring the weight of a sample while the sample is in the cavity and on the balance, a temperature sensor capable of measuring and positioned to measure the temperature of a sample in the cavity and on the balance, and a processor in communication with the temperature sensor and each of the first and second sources for controlling the introduction of the frequencies of microwave and radiant energy into the cavity in response to the temperatures measured by the temperature sensor to control the sample temperature until the microwaves from the first source and the radiant heat from the second source dry the sample sufficiently for the processor to determine the volatile content of the sample based on the weight change of the sample on the balance.
G01N 5/04 - Analyse des matériaux par pesage, p.ex. pesage des fines particules séparées d'un gaz ou d'un liquide en éliminant un constituant, p.ex. par évaporation, et en pesant le reste
H01J 25/00 - Tubes à temps de transit, p.ex. klystrons, tubes à ondes progressives, magnétrons
85.
Microwave enhanced N-fmoc deprotection in peptide synthesis
A method is disclosed for carrying out peptide synthesis comprising deprotecting an Fmoc-protected amino acid with piperazine while applying microwave irradiation to the deprotection reaction.
A method of accelerating the hydrogenation of organic compounds is provided. The method includes positioning an microwave transparent reaction vessel containing at least one reactant suitable for hydrogenation in a microwave cavity, purging the reaction vessel, charging the reaction vessel with hydrogen gas, and applying a continuous single mode of microwave radiation within the cavity and to the vessel and its contents for a time sufficient to effect a chemical change in the reactants.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
C07C 5/02 - Préparation d'hydrocarbures à partir d'hydrocarbures contenant le même nombre d'atomes de carbone par hydrogénation
C07C 29/17 - Préparation de composés comportant des groupes hydroxyle ou O-métal liés à un atome de carbone ne faisant pas partie d'un cycle aromatique à six chaînons par hydrogénation de liaisons doubles ou triples carbone-carbone
C07C 67/303 - Préparation d'esters d'acides carboxyliques par modification de la partie acide de l'ester sans introduction d'un groupe ester par hydrogénation de liaisons non saturées carbone-carbone
C07C 209/26 - Préparation de composés contenant des groupes amino liés à un squelette carboné par alkylation réductive, avec des composés carbonylés, d'ammoniac, d'amines ou de composés ayant des groupes réductibles en groupes amino par réduction avec de l'hydrogène
C07C 209/36 - Préparation de composés contenant des groupes amino liés à un squelette carboné par réduction de liaisons azote-oxygène ou azote-azote par réduction de groupes nitro par réduction de groupes nitro liés à des atomes de carbone de cycles aromatiques à six chaînons
C07D 207/16 - Atomes de carbone comportant trois liaisons à des hétéro-atomes, avec au plus une liaison à un halogène, p.ex. radicaux ester ou nitrile
C07J 9/00 - Stéroïdes normaux contenant du carbone, de l'hydrogène, un halogène ou de l'oxygène, substitués en position 17bèta par une chaîne de plus de deux atomes de carbone, p.ex. cholane, cholestane, coprostane
H05B 6/80 - Appareils pour applications spécifiques
87.
Method and apparatus for conducting microwave assisted organic reactions with gas-phase reactants
A method of accelerating the hydrogenation of organic compounds is provided. The method includes positioning an microwave transparent reaction vessel containing at least one reactant suitable for hydrogenation in a microwave cavity, purging the reaction vessel, charging the reaction vessel with hydrogen gas, and applying a continuous single mode of microwave radiation within the cavity and to the vessel and its contents for a time sufficient to effect a chemical change in the reactants.
A method of accelerating the hydrogenation of organic compounds is provided. The method includes positioning an microwave transparent reaction vessel containing at least one reactant suitable for hydrogenation in a microwave cavity, purging the reaction vessel, charging the reaction vessel with hydrogen gas, and applying a continuous single mode of microwave radiation within the cavity and to the vessel and its contents for a time sufficient to effect a chemical change in the reactants.
C07C 209/26 - Préparation de composés contenant des groupes amino liés à un squelette carboné par alkylation réductive, avec des composés carbonylés, d'ammoniac, d'amines ou de composés ayant des groupes réductibles en groupes amino par réduction avec de l'hydrogène
C07C 209/36 - Préparation de composés contenant des groupes amino liés à un squelette carboné par réduction de liaisons azote-oxygène ou azote-azote par réduction de groupes nitro par réduction de groupes nitro liés à des atomes de carbone de cycles aromatiques à six chaînons
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
C07C 5/03 - Préparation d'hydrocarbures à partir d'hydrocarbures contenant le même nombre d'atomes de carbone par hydrogénation de liaisons doubles carbone-carbone non aromatiques
C07C 29/17 - Préparation de composés comportant des groupes hydroxyle ou O-métal liés à un atome de carbone ne faisant pas partie d'un cycle aromatique à six chaînons par hydrogénation de liaisons doubles ou triples carbone-carbone
C07C 67/303 - Préparation d'esters d'acides carboxyliques par modification de la partie acide de l'ester sans introduction d'un groupe ester par hydrogénation de liaisons non saturées carbone-carbone
C07D 207/08 - Composés hétérocycliques contenant des cycles à cinq chaînons, non condensés avec d'autres cycles, ne comportant qu'un atome d'azote comme unique hétéro-atome du cycle avec uniquement des atomes d'hydrogène ou de carbone liés directement à l'atome d'azote du cycle ne comportant pas de liaison double entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques avec des radicaux hydrocarbonés, substitués par des hétéro-atomes, liés aux atomes de carbone du cycle
A method is disclosed for carrying out peptide synthesis comprising deprotecting an Fmoc-protected amino acid with piperazine while applying microwave irradiation to the deprotection reaction.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Chemicals used in industry and science, namely, concentrated dye solutions and diluted dye solutions suitable for dye-binding protein analysis reactions
92.
Real-time imaging and spectroscopy during microwave assisted chemistry
An instrument and associated method are disclosed for conducting microwave assisted chemical reactions. The instrument includes a microwave cavity, preferably a closed microwave cavity, for conducting microwave assisted chemical reactions, and a source for applying microwave radiation within the cavity and to a vessel and its contents. The instrument also includes an illumination source for illuminating the vessel and its contents, as well as a camera or spectrometer for visually observing the vessel and its contents, an infrared detector for monitoring the temperature of the vessel and its contents, and a filter for preventing the illumination source from saturating the infrared detector, thereby enabling concurrent visual observation and infrared monitoring.
An instrument and associated method are disclosed for conducting microwave assisted chemical reactions. The instrument includes a microwave cavity, preferably a closed microwave cavity, for conducting microwave assisted chemical reactions, and a source for applying microwave radiation within the cavity and to a vessel and its contents. The instrument also includes an illumination source for illuminating the vessel and its contents, as well as means for visually observing the vessel and its contents, an infrared detector for monitoring the temperature of the vessel and its contents, and means for preventing the illumination source from saturating the infrared detector, thereby enabling concurrent visual observation and infrared monitoring.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
An instrument and process for accelerating the solid phase synthesis of peptides is disclosed. The method includes the steps of deprotecting a protected first amino acid linked to a solid phase resin by admixing the protected linked acid with a deprotecting solution in a microwave transparent vessel while irradiating the admixed acid and solution with microwaves, then activating a second amino acid by adding the second acid and an activating solution to the same vessel while irradiating the vessel with microwaves, then coupling the second amino acid to the first acid while irradiating the composition in the same vessel with microwaves, and cleaving the linked peptide from the solid phase resin by admixing the linked peptide with a cleaving composition in the same vessel while irradiating the composition with microwaves.
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
C07K 1/06 - Procédés généraux de préparation de peptides utilisant des groupes protecteurs ou des agents d'activation
An instrument and process for accelerating the solid phase synthesis of peptides is disclosed. The method includes the steps of deprotecting a protected first amino acid linked to a solid phase resin by admixing the protected linked acid with a deprotecting solution in a microwave transparent vessel while irradiating the admixed acid and solution with microwaves, then activating a second amino acid by adding the second acid and an activating solution to the same vessel while irradiating the vessel with microwaves, then coupling the second amino acid to the first acid while irradiating the composition in the same vessel with microwaves, and cleaving the linked peptide from the solid phase resin by admixing the linked peptide with a cleaving composition in the same vessel while irradiating the composition with microwaves.
An instrument and process for accelerating the solid phase synthesis of peptides is disclosed. The method includes the steps of deprotecting a protected first amino acid linked to a solid phase resin by admixing the protected linked acid with a deprotecting solution in a microwave transparent vessel while irradiating the admixed acid and solution with microwaves, then activating a second amino acid by adding the second acid and an activating solution to the same vessel while irradiating the vessel with microwaves, then coupling the second amino acid to the first acid while irradiating the composition in the same vessel with microwaves, and cleaving the linked peptide from the solid phase resin by admixing the linked peptide with a cleaving composition in the same vessel while irradiating the composition with microwaves.
C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaire; Appareils à cet usage utilisant des radiations électromagnétiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
C07K 1/06 - Procédés généraux de préparation de peptides utilisant des groupes protecteurs ou des agents d'activation
09 - Appareils et instruments scientifiques et électriques
Produits et services
SCIENTIFIC INSTRUMENTATION, COMPRISING MICROWAVE BASED INSTRUMENTS FOR TESTING SAMPLES AND PARTS THEREOF; AND CONSUMABLE SUPPLIES COMPRISING VESSELS FOR SAMPLE DIGESTION AND PADS FOR WEIGHING SAMPLES
