Systems for processing a fluid sample to facilitate analysis with a semiconductor detection chip are provided herein. Such systems can include a sample processing cartridge coupleable with a chip carrier device configured for transport of the processed fluid sample from the sample cartridge. The chip carrier device can include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the sample processing cartridge. The chip carrier device can include multiple portions or adapters, including a fluid sample portion, a flowcell portion and a chip carrier. Also provided are methods of preparing and transporting a fluid sample from a sample cartridge into a chip carrier device for analysis with a semiconductor detection chip carried within the chip carrier device.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
A system and method for manufacturing molded components having one or more curved channels. When a mold is in closed, a retractable pin assembly may be used to advance one or more curved pins into a mold. When a mold is opened, the retractable pin assembly may retract the one or more curved pins from the mold. The molded component formed in the mold can have one or more curved channels having the same shape as the one or more curved pins.
A system and method for manufacturing chip carrier devices. A chip carrier device frame may be injection molded. A gasket and an analysis chip may be placed in the chip carrier device frame and retained with a retainer. The chip carrier device can have one or more fluidic channels to transport a fluidic sample to the analysis chip.
A biological sample processing apparatus having a plurality of bays configured interchangeably reactive any of a plurality of module types configured to independently receive and perform analysis on a sample cartridge assembly. Simultaneous access of one or more modules can be accomplished through different translation or pivoting of different portions of the front of the instrument. Advanced filter systems and servicing methods are also provided.
Described herein are methods, systems, and devices for amplifying nucleic acids via ultrafast thermal convection processes along with cartridge-based methods, systems, and devices directed to the same. The methods, systems, and devices are useful for 5 polymerase chain reactions for detection and diagnosis of infectious diseases.
Described herein are methods of amplifying nucleic acids via ultrafast thermal convection processes along with cartridge-based methods directed to the same. The methods are useful for polymerase chain reactions for detection and diagnosis of infectious diseases.
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
7.
INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
Methods of determining methylation of DNA are provided that include filtering a biological sample comprising a nucleic acid with a first matrix material to purify the DNA; eluting and denaturing the DNA to produce eluted denatured DNA; heating the DNA in the presence of bisulfite ions to produce deaminated nucleic acid; optionally contacting said deaminated nucleic acid with a second matrix material; desulphonating and eluting the bound deaminated nucleic acid; and performing methylation specific PCR, nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein multiple steps may be performed in a single reaction cartridge.
Described herein are aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
9.
DIAGNOSTIC DETECTION CHIP DEVICES AND METHODS OF MANUFACTURE AND ASSEMBLY
Diagnostic detection chip device designs that reduce cost of fabrication and assembly are described herein. Such chip device designs include features that facilitate use of the chip within a chip carrier device with integrated fluid flow control features and compatibility with conventional sample cartridges and sample processing systems. Associated methods of manufacture and assembly of the chip devices are also provided herein.
Methods and systems for estimating force and motor torque in a mechatronic system are provided herein. Such methods and system are suited for improved control of small-scale mechatronic system, particularly a syringe, valve, and cartridge loader or door opening/closing mechanism of a diagnostic assay system. The methods can compensate for friction and account for various second-order effects, thereby allowing for more accurate pressure estimation, thereby allowing improved syringe operation. The methods can further allow for improved estimation of force or motor torque to allow for improved control of an actuatable valve interfacing the sample cartridge and cartridge loader or door opening/closing system. Methods of calibrating such systems are also provided.
A61M 5/172 - Moyens pour commander l'écoulement des agents vers le corps ou pour doser les agents à introduire dans le corps, p. ex. compteurs de goutte-à-goutte électriques ou électroniques
A61M 5/168 - Moyens pour commander l'écoulement des agents vers le corps ou pour doser les agents à introduire dans le corps, p. ex. compteurs de goutte-à-goutte
12.
DIAGNOSTIC ASSAY SYSTEM WITH REPLACEABLE PROCESSING MODULES AND REMOTE MONITORING
A biological sample processing apparatus having an enclosure and a plurality of sample processing modules held within an enclosure with a tiltable graphical user interface screen. In one aspect, the individual modules that are readily removable for repair, replacement or upgrade. Each module is configured to be independently operable and readily inserted into the enclosure for connection with a processing unit of the enclosure. Each module can include quick-release mechanisms so that the module can be readily removed and replaced manually or with minimal tools through the front of the enclosure without requiring substantial or total disassembly of the module or entire enclosure. In another aspect, the user interface screen can display identifying information, such as a barcode, that can be scanned by a user's portable device so as to monitor the progress of an assay remotely.
An analytic system for carrying out a chemical assay, the system having a bay with an opening on one side, the bay of a size and shape to enclose a cartridge carrying a sample material to be analyzed, one or more mechanisms within the bay through which the cartridge and or material within the cartridge is influenced, a door of a size to cover the opening, a securing mechanism associated with the bay and the door, by which the door is secured when closed.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
The present disclosure provides pancoronavirus biomarker panels that can be used to detect a wide variety of characterized coronavirus strains and, in some embodiments, emerging or novel coronavirus strains. In certain embodiments, this detection can be carried out in a single, highly multiplexed nucleic acid amplification assay.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C07K 14/165 - Coronaviridae, p. ex. virus de la bronchite infectieuse aviaire
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
15.
SAMPLE IN SEQUENCING-READY LIBRARY OUT AUTOMATED LIBRARY PREPARATION SOLUTION
The present disclosure generally relates to cartridge-based methods and devices for preparing a sequencing nucleic acid library from a sample. In embodiments, a cartridge-based method comprises placing a sample in a self-contained cartridge having a plurality of chambers, a reaction vessel, and a filter disposed in a fluidic path between the plurality of chambers and the reaction vessel, utilizing the filter to isolate the nucleic acid from the sample; forming a library prep reaction mixture; causing the library prep reaction mixture to flow into the reaction vessel and preparing the sequencing nucleic acid library comprising the tagged nucleic acid fragments, amplicons, and/or adapter labeled nucleic acid; isolating the tagged nucleic acid fragments, amplicons, and/or adapter labeled nucleic acid from the library prep reaction mixture on to the filter and eluting each from the filter; and purifying and optionally size selecting the sequencing nucleic acid library on the filter.
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
17.
Led Characterization and Compensation Methods and Systems
Systems and methods for monitoring, characterizing and controlling operation of LEDs are provided herein. Methods includes measuring a voltage across the LED, and correlating the voltage to a junction temperature of the LED. This correlation can be used to improve operation of the LED by increasing the signal to noise ratio of the LED signal, characterize the LED by comparing to an I-V curve, control LED operation to compensate for LED degradation and avoid crosstalk, and/or to generally improve performance and life expectancy of the LED. Improved performance of the LED can include stabilizing the photon output during performance of an assay to provide a desired dye reporter signal required for the assay and/or reducing an intra-shot during of the LED output during the assay. System and device with control units configured to perform these methods are also described herein.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
18.
METHODS AND COMPOSITIONS FOR NUCLEIC ACID ISOLATION
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
GLOBAL LIFE SCIENCES SOLUTIONS CANADA ULC (Canada)
Inventeur(s)
Declue, Michael
Harvie, Pierrot
Nakatani, Cameron J.
Abrégé
PCR methods and compositions are described for using a liposomal particle disclosed herein. In one embodiment, the liposomal particle comprises at least one lipid and at least one PCR reaction reagent, where the at least one lipid encapsulates the at least one PCR reaction reagent. The liposomal particles can be used to deliver specific PCR reaction reagents with precise accuracy using temperature as a regulator.
A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container. The bottom-cap further includes one or more auxiliary ports for injecting a reagent when the lid apparatus is in a closed configuration sealingly attached to the multi-chambered sample container.
B65D 47/08 - Fermetures avec dispositifs de décharge autres que des pompes avec goulottes ou tubes de déversementFermetures avec dispositifs de décharge autres que des pompes avec tuyères ou passages pour la décharge ayant des fermetures articulées ou à charnières
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
F25B 21/02 - Machines, installations ou systèmes utilisant des effets électriques ou magnétiques utilisant l'effet PeltierMachines, installations ou systèmes utilisant des effets électriques ou magnétiques utilisant l'effet Nernst-Ettinghausen
G01K 1/02 - Moyens d’indication ou d’enregistrement spécialement adaptés aux thermomètres
G01K 13/024 - Thermomètres spécialement adaptés à des fins spécifiques pour mesurer la température de fluides en mouvement ou de matériaux granulaires capables de s'écouler de gaz en mouvement
G01N 1/42 - Traitement à basse température des échantillons, p. ex. cryofixation
G01N 1/44 - Traitement d'échantillons mettant en œuvre un rayonnement, p. ex. de la chaleur
Thermal control devices adapted to provide improved control and efficiency in temperature cycling are provided herein. Such thermal control device can include a thermoelectric cooler controlled in coordination with another thermal manipulation device to control an opposing face of the thermoelectric cooler and/or a microenvironment. Some such thermal control devices include a first and second thermoelectric cooler separated by a thermal capacitor. The thermal control devices can be configured in a planar configuration with a means for thermally coupling with a planar reaction vessel of a sample analyzer for use in thermal cycling in a polymerase chain reaction of the fluid sample in the reaction vessel. Methods of thermal cycling using such a thermal control devices are also provided.
F25B 21/04 - Machines, installations ou systèmes utilisant des effets électriques ou magnétiques utilisant l'effet PeltierMachines, installations ou systèmes utilisant des effets électriques ou magnétiques utilisant l'effet Nernst-Ettinghausen réversibles
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
26.
SYSTEMS AND METHODS FOR IDENTIFYING BIOMARKERS USING CONTINUOUS GENE EXPRESSION PROFILES
Systems and methods for identifying biomarkers using continuous gene expression profiles are provided. In example aspects, non-continuous gene expression data for a patient is converted into a continuous gene expression profile. Genes indicative of a patient condition are selected based on the continuous gene expression profile. In an example, a selection model selects a plurality of combinations of genes based on the continuous gene expression profile, and a plurality of classification models are trained based on the selected genes. The results of the classification models are used to determine biomarkers.
The present disclosure relates to isothermal amplification using novel synthetic oligonucleotides useful as primers and probes. These oligonucleotides are particularly useful in isothermal amplification methods wherein a 3'-blocked primer is activated by an endo-ribonuclease, such as RNase H1. The novel synthetic oligonucleotides include one or more 3'-modified ribonucleotides to control where in the oligonucleotides RNase H1 cleaves. The preferred isothermal amplification uses a strand-displacement polymerase, such as Bst 3.0, and cycling probe technology, which may also use RNase H1, for detection. The disclosure also provides combinations of primers, optionally within a cartridge, as well as a method, optionally carried out within a cartridge, and related kits.
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a solid support and an affinity reagent for isolating and detecting the nucleic acid.
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a solid support and an affinity reagent for isolating and detecting the nucleic acid.
C12Q 1/6893 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les protozoaires
Improved sub-assemblies and methods of control for use in a diagnostic assay system adapted to receive an assay cartridge are provided herein. Such sub-assemblies include: a brushless DC motor, a door opening/closing mechanism and cartridge loading mechanism, a syringe and valve drive mechanism assembly, a sonication horn, a thermal control device and optical detection/excitation device. Such systems can further include a communications unit configured to wirelessly communicate with a mobile device of a user so as to receive a user input relating to functionality of the system with respect to an assay cartridge received therein and relaying a diagnostic result relating to the assay cartridge to the mobile device.
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
H02P 6/182 - Dispositions de circuits pour détecter la position sans éléments séparés pour détecter la position utilisant la force contre-électromotrice dans les enroulements
H02P 27/06 - Dispositions ou procédés pour la commande de moteurs à courant alternatif caractérisés par le type de tension d'alimentation utilisant une tension d’alimentation à fréquence variable, p. ex. tension d’alimentation d’onduleurs ou de convertisseurs utilisant des convertisseurs de courant continu en courant alternatif ou des onduleurs
32.
Fluidic Bridge Device and Sample Processing Methods
A fluidic bridge device for transport of a fluid sample between a first sample processing device and a second sample processing device. The fluidic bridge may include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the first and second sample processing device. In one aspect, the first device is a sample preparation device and the second device is an assay device. The fluidic bridge can include at least two fluid conduits, at least one for transport of the prepared sample, and at least one other to facilitate displacement of air to allow flow of the prepared sample through the other fluid conduit. The fluid channels can include one or more of an amplification chamber, a processing chamber, a gas-permeable vent, a bubble trap, a filter, and an external port. Methods of preparing and transporting a fluid sample between devices are provided herein.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
33.
MAXIMIZING OPTICAL CHANNEL CAPABILITIES FOR TARGET DETECTION
Compositions, methods, and systems for detecting melt temperature signatures for at least two target regions of nucleic acid in a multiplex amplification reaction are provided. The methods include contacting the nucleic acid with sets of primers and sets of two or more probes for detecting each target region, wherein each of the two or more probes in a set comprises a detectable label that emits light at the same wavelength; subjecting the nucleic acid, primers, and probes to amplification conditions to amplify the target regions; and generating and detecting a melt temperature signature specific for each target region present, wherein the melt temperature signature comprises a plurality of melt profiles generated from the set of two or more probes hybridizing to the amplified target region.
The present disclosure provides a method for differentiating genotypes of nucleic acid in a multiplex amplification reaction. The method includes generating one or more melt curve profiles for each target region of nucleic acid, classifying the melt curve profiles into genotype cluster(s), wherein classifying is performed by normalizing and calculating a derivative for each melt curve profile to form a processed melt curve profile, creating a density map using data from each processed melt curve profile as coordinates for the density map, wherein each genotype forms a genotype cluster in the density map and the proximity of each genotype cluster to another genotype cluster is determined by the similarity of the genotype's processed melt curve profile, and differentiating and optionally identifying the genotypes of nucleic acid based on classification of the melt curve profiles.
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
35.
DIAGNOSTIC CHIP CARRIER DEVICE WITH GASKET AND METHODS OF MANUFACTURE
Diagnostic chip devices, and methods and devices for assembly and manufacture, are described. Such chip device includes a chip carrier device that includes a frame having a fluidic interface, one or more fluidic channels and a gasket that forms a flowcell sealed against an active surface of a diagnostic chip. The gasket can be overmolded into the frame, or can be separately applied and compressed by the chip, which can be secured to the frame by cold swaging, hot swaging or heat staking, adhesive or any suitable means. The chip carrier device can further include a window over the flowcell to allow for optical detection of the active face of the chip. The window can be formed by a transparent lid or glass insert attached to the frame by laser welding, overmolding, adhesive or any suitable means. The carrier and lid can also be formed as a single integral component.
A handling system for high throughput processing of a large volume of biological samples is provided herein. Such systems can include an array support assembly that supports multiple diagnostic assay modules in an array having at least two dimensions, a loader that loads multiple diagnostic assay cartridges within the multiple diagnostic assay modules. The array support assembly can be movable relative the loader to facilitate loading and unloading so as to provide more efficient processing.
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Integrated valve body and syringe tube components to facilitate controlled fluid flow in assay cartridges are provided herein. Such components can include a valve body and syringe tube that are integrally formed as a single component. Components can include a syringe tube having a conduit in fluid communication with a valve body having a lysing chamber and a set of ports to facilitate direct fluid flow between chambers and fluid flow through the lysing chamber of the valve body. Valve body can be sealed by a cap that is welded to the valve body. Such components can be configured for use within sample cartridges configured for mechanical lysing, or for both mechanical and chemical lysing.
Electrical interface devices for passively engaging with a chip device to facilitate operation of a diagnostic chip are provided herein. chip. Such devices can include a bridge connector having pin contacts thereon that is pivotally mounted within a processing module that passively actuated upon insertion of the chip device to deploy the pin contacts and provide controlled engagement with corresponding contact pads of the chip device. Such bridge connectors can include cam(s) having cam surface(s) designed to facilitate rotation of the connector to a deployed configuration and controlled engagement of the pin contacts to facilitate a scrubbing action of the pin contacts with the contact pads. Methods of assembly and use are also provided herein.
Diagnostic chip devices, and methods and devices for assembly and manufacture, are described. Such chip device includes a chip carrier device that includes a frame having a fluidic interface, one or more fluidic channels and a gasket that forms a flowcell sealed against an active surface of a diagnostic chip. The gasket can be overmolded into the frame, or can be separately applied and compressed by the chip, which can be secured to the frame by cold swaging, hot swaging or heat staking, adhesive or any suitable means. The chip carrier device can further include a window over the flowcell to allow for optical detection of the active face of the chip. The window can be formed by a transparent lid or glass insert attached to the frame by laser welding, overmolding, adhesive or any suitable means. The carrier and lid can also be formed as a single integral component.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor relative the substrate to be determined from linear portions of the sinusoidal signal without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02K 1/2791 - Aimants montés en surfaceAimants sertis
H02K 11/00 - Association structurelle de machines dynamo-électriques à des organes électriques ou à des dispositifs de blindage, de surveillance ou de protection
H02K 11/215 - Dispositifs utilisant un effet magnétique, p. ex. des éléments à effet Hall ou magnéto-résistifs
H02K 15/02 - Procédés ou appareils spécialement adaptés à la fabrication, l'assemblage, l'entretien ou la réparation des machines dynamo-électriques des corps statoriques ou rotoriques
H02K 21/22 - Moteurs synchrones à aimants permanentsGénératrices synchrones à aimants permanents avec des induits fixes et des aimants tournants avec des aimants tournant autour des induits, p. ex. volants magnétiques
H02K 29/08 - Moteurs ou génératrices à dispositifs de commutation non mécaniques, p. ex. tubes à décharge ou dispositifs à semi-conducteurs avec des dispositifs détecteurs de la position utilisant des dispositifs à effet magnétique, p. ex. dispositifs à effet Hall ou magnéto-résistances
42.
EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH REDUCED AMPLIFICATION TIME
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising modifying the aptamer by uracil-DNA glycosylase enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. In some aspects, the oligonucleotide aptamers are circular and comprise one or more deoxyuridine nucleotides providing for aptamer-specific recognition and modification of the circular aptamer by the uracil-DNA glycosylase enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing uracil-DNA glycosylase enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one uracil-DNA glycosylase enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific modification of the aptamer by the uracil-DNA glycosylase enzymatic activity.
Compositions, methods, and systems for detecting human epidermal growth factor receptor 2 (HER2)-low expression levels in a biological sample are disclosed. Also disclosed are compositions, methods, and systems for distinguishing HER2-low expression from HER2-negative or HER2-positive expression. Compositions for improved RNA recovery during sample preparation steps and detection of nucleic acids in the methods herein are also disclosed.
Described herein is a comprehensive novel amplification system that can employ novel nucleic acid constructs as blocked primers, such constructs including a target-specific region, wherein the target-specific region comprises a target-specific cleavage domain, and a universal adapter sequence located 5′ of the target-specific region, wherein the universal adapter sequence comprises a non-natural nucleotide base. Also described is a fusion protein between a polymerase and an endonuclease, which is useful in this system, as well as related methods and kits.
Thermal control devices utilizing a thermal switch to facilitate cooling of a biological sample, particularly a biological sample tested with a semiconductor diagnostic detection chip. Such thermal switches can include a mass of thermally conductive material, such as copper or aluminum, that selectively contacts the diagnostic chip or sample tube by use of an air cylinder or a servo-driven moveable support. Alternatively, the thermal switch can utilize a thermally conductive material that selectively contacts the diagnostic chip or sample tube by use of an inflatable bladder. Alternatively, the thermal switch can utilize a voice coil and a heat sink to selectively contact the diagnostic chip. The thermal control device can further include thermal overshoot feature, such as an air blower or placement of a heat sink in close proximity to the chip or tube. Associated methods of assembly and use of thermal control devices are also provided herein.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01K 13/12 - Thermomètres spécialement adaptés à des fins spécifiques combinés avec des dispositifs d'échantillonnage pour mesurer les températures des échantillons du matériau
48.
THERMAL SWITCH FOR DIAGNOSTIC DETECTION CHIP DEVICES AND ASSOCIATED METHODS OF MANUFACTURE AND USE
Thermal control devices utilizing a thermal switch to facilitate cooling of a biological sample, particularly a biological sample tested with a semiconductor diagnostic detection chip. Such thermal switches can include a mass of thermally conductive material, such as copper or aluminum, that selectively contacts the diagnostic chip or sample tube by use of an air cylinder or a servo-driven moveable support. Alternatively, the thermal switch can utilize a thermally conductive material that selectively contacts the diagnostic chip or sample tube by use of an inflatable bladder. Alternatively, the thermal switch can utilize a voice coil and a heat sink to selectively contact the diagnostic chip. The thermal control device can further include thermal overshoot feature, such as an air blower or placement of a heat sink in close proximity to the chip or tube. Associated methods of assembly and use of thermal control devices are also provided herein.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Downloadable and recorded operating system software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; downloadable and recorded software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for collecting, managing, storing, analyzing, and reporting data generated by the apparatus and instruments; downloadable and recorded operating software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; downloadable and recorded software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; downloadable and recorded software for collecting, managing, storing, analyzing, and reporting biological and medical information; downloadable and recorded software used for collecting, managing, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; downloadable and recorded data analysis software for laboratory, scientific, research, and medical use; downloadable and recorded computer software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; downloadable and recorded laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; downloadable and recorded computer programs, namely, computer programs for medical monitoring and analysis of biological and medical data; downloadable and recorded software for clinical decision support; downloadable and recorded software for providing medical information to a clinician; downloadable and recorded software for data analysis and clinical decision support for health care; downloadable and recorded software using artificial intelligence for data analysis and clinical decision support for health care. Software as a service (SAAS) services featuring software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; Software as a service (SAAS) services featuring software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for uploading, collecting, managing, storing, analyzing, sharing, reporting, and preserving data generated by the apparatus and instruments; Software as a service (SAAS) services featuring software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; Software as a service (SAAS) services featuring software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; Software as a service (SAAS) services featuring software for collecting, managing, storing, analyzing, and reporting biological and medical information; Software as a service (SAAS) services featuring software for collecting, managing, aggregating, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; Software as a service (SAAS) services featuring data analysis software for laboratory, scientific, research, and medical use; Software as a service (SAAS) services featuring software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; Software as a service (SAAS) services featuring laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; Software as a service (SAAS) services featuring software for medical monitoring and analysis of biological and medical data; Providing temporary use of non-downloadable medical analysis software for use in medical diagnostics; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data to predict epidemiological trends; providing online non-downloadable software for clinical decision support; providing temporary use of non-downloadable software for providing medical information to a clinician; providing on-line non-downloadable software for data analysis and clinical decision support for health care; software as a service (SAAS) services featuring software using artificial intelligence for data analysis and clinical decision support for health care.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Downloadable and recorded computer software platforms for operating, controlling, and managing molecular diagnostic testing instruments and for collecting, storing, and reporting diagnostic test results in medical laboratory settings; downloadable and recorded computer software for processing and interpreting results from in vitro molecular diagnostic tests on biological samples for clinical use in the detection of infectious diseases, oncological markers, and genetic variants; computer operating programs; downloadable and recorded operating system software for use in connection with molecular diagnostic testing instruments for analyzing biological samples and bodily fluids; downloadable and recorded software for use in operating, monitoring, and controlling molecular diagnostic testing instruments for collecting, managing, storing, analyzing, and reporting data generated by the molecular diagnostic testing instruments; downloadable and recorded operating software for use with molecular diagnostic testing instruments, namely, for use in providing automation of laboratory processes, control and monitoring of molecular diagnostic testing instruments, molecular diagnostic testing instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; downloadable and recorded software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of molecular diagnostic testing instruments; downloadable and recorded software for collecting, managing, storing, analyzing, and reporting biological and medical information; downloadable and recorded software used for collecting, managing, storing, analyzing, and reporting data from molecular diagnostic testing instruments; downloadable and recorded computer software for analyzing nucleic acid amplification data and generating qualitative and quantitative diagnostic results from molecular diagnostic tests performed on biological samples; downloadable and recorded computer software using artificial intelligence for managing and analyzing data from molecular diagnostic testing instruments; downloadable and recorded laboratory middleware software for providing an interface between laboratory information systems (LIS), physician information systems, hospital information systems (HIS), electronic medical record systems (EMR) and molecular diagnostic testing instruments; downloadable and recorded computer programs, namely, computer programs for monitoring the operational status and performance of molecular diagnostic testing instruments and for tracking and recording the results of in vitro diagnostic tests on biological samples and analysis of biological and medical data; downloadable and recorded computer software for providing clinicians with molecular diagnostic test results and interpretive data to support treatment decisions in the management of infectious diseases, respiratory infections, and oncological conditions; downloadable and recorded software for providing medical information to a clinician; downloadable and recorded computer software for analyzing in vitro molecular diagnostic test results and supporting clinical decision-making in the diagnosis and management of infectious diseases, respiratory conditions, and oncological conditions; downloadable and recorded computer software using artificial intelligence for analyzing nucleic acid amplification test results and epidemiological data from molecular diagnostic testing instruments to support clinician decision-making in the diagnosis and management of infectious diseases and oncological conditions (1) Software as a service (SaaS) services featuring software for operating, controlling, and managing molecular diagnostic testing instruments and for collecting, storing, and reporting molecular diagnostic test results; providing online non-downloadable computer software for use in operating, monitoring, and managing molecular diagnostic testing instruments for the detection of pathogens and genetic markers in biological samples; platform as a service (PaaS) services featuring a cloud-based software platform for connecting molecular diagnostic testing instruments to laboratory information systems (LIS) and hospital information systems (HIS) for the purpose of aggregating, storing, and reporting diagnostic test results; computer software consultancy; computer software design; maintenance of computer software; updating of computer software; Software as a service (SAAS) services featuring software for use in connection with molecular diagnostic testing instruments for analyzing biological samples and bodily fluids; Software as a service (SAAS) services featuring software for use in operating, monitoring, and controlling molecular diagnostic testing instruments for uploading, collecting, managing, storing, analyzing, sharing, reporting, and preserving data generated by the molecular diagnostic testing instruments; Software as a service (SAAS) services featuring software for use with molecular diagnostic testing instruments, namely, for use in providing automation of laboratory processes, control and monitoring of molecular diagnostic testing instruments, molecular diagnostic testing instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; Software as a service (SAAS) services featuring software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of molecular diagnostic testing instruments; Software as a service (SAAS) services featuring software for collecting, managing, storing, analyzing, and reporting biological and medical information; Software as a service (SAAS) services featuring software for collecting, managing, aggregating, storing, analyzing, and reporting data from molecular diagnostic testing instruments; Software as a service (SaaS) services featuring software for analyzing nucleic acid amplification data and generating qualitative and quantitative diagnostic results from molecular diagnostic tests performed on biological samples; Software as a service (SAAS) services featuring software using artificial intelligence for managing and analyzing data from molecular diagnostic testing instruments; Software as a service (SAAS) services featuring laboratory middleware software for providing an interface between laboratory information systems (LIS), physician information systems, hospital information systems (HIS), electronic medical record systems (EMR) and molecular diagnostic testing instruments; Software as a service (SAAS) services featuring software for monitoring the operational status and performance of molecular diagnostic testing instruments and for tracking and recording the results of in vitro diagnostic tests on biological samples and analysis of biological and medical data; providing temporary use of non-downloadable computer software for analyzing nucleic acid amplification test data from in vitro molecular diagnostic tests performed on biological samples for the qualitative and quantitative detection of infectious disease pathogens, oncological markers, and genetic variants; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data to predict epidemiological trends; providing online non-downloadable computer software for supplying clinicians with molecular diagnostic test results and evidence-based interpretive guidance to support treatment decisions in the management of infectious diseases, respiratory infections, and oncological conditions; providing temporary use of non-downloadable software for providing medical information to a clinician; providing online non-downloadable computer software for analyzing in vitro molecular diagnostic test results and supporting clinical decision-making in the diagnosis and management of infectious diseases, respiratory conditions, and oncological conditions; software as a service (SaaS) services featuring software using artificial intelligence for analyzing nucleic acid amplification test results and epidemiological data from molecular diagnostic testing instruments to support clinician decision-making in the diagnosis and management of infectious diseases and oncological conditions
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
downloadable and recorded operating software for use with laboratory and diagnostic instruments, namely, for use in setting up experiment protocols; downloadable and recorded computer software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; downloadable and recorded laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; downloadable and recorded computer programs, namely, computer programs for medical monitoring; downloadable and recorded software for clinical decision support; downloadable and recorded software for data analysis and clinical decision support for health care; downloadable and recorded software using artificial intelligence for data analysis and clinical decision support for health care Software as a service (SAAS) services featuring software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; Software as a service (SAAS) services featuring software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for uploading, collecting, managing, storing, analyzing, sharing, reporting, and preserving data generated by the apparatus and instruments; Software as a service (SAAS) services featuring software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management; Software as a service (SAAS) services featuring software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; Software as a service (SAAS) services featuring laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; Providing temporary use of non-downloadable medical analysis software for use in medical diagnostics; providing online non-downloadable software for clinical decision support; providing on-line non-downloadable software for data analysis and clinical decision support for health care; software as a service (SAAS) services featuring software using artificial intelligence for data analysis and clinical decision support for health care
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
downloadable and recorded operating system software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; downloadable and recorded software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for collecting, managing, storing, analyzing, and reporting data generated by the apparatus and instruments; downloadable and recorded operating software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, sample management, connectivity, and data, collection, analysis, management, and reporting; downloadable and recorded software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; downloadable and recorded software for collecting, managing, storing, analyzing, and reporting biological and medical information; downloadable and recorded software used for collecting, managing, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; downloadable and recorded data analysis software for laboratory, scientific, research, and medical use; downloadable and recorded computer programs, namely, computer programs for analysis of biological and medical data; downloadable and recorded software for providing medical information to a clinician Software as a service (SAAS) services featuring software for use with laboratory and diagnostic instruments, namely, for use in connectivity, and data, collection, analysis, management, and reporting; Software as a service (SAAS) services featuring software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; Software as a service (SAAS) services featuring software for collecting, managing, storing, analyzing, and reporting biological and medical information; Software as a service (SAAS) services featuring software for collecting, managing, aggregating, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; Software as a service (SAAS) services featuring data analysis software for laboratory, scientific, research, and medical use; Software as a service (SAAS) services featuring software for medical monitoring and analysis of biological and medical data; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data to predict epidemiological trends; providing temporary use of non-downloadable software for providing medical information to a clinician
53.
THREE-PHASE NESTED AMPLIFICATION AND MULTI-PHASIC DETECTION
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
55.
INTEGRATED VALVE BODY AND SYRINGE TUBE COMPONENT FOR ASSAY CARTRIDGES
Integrated valve body and syringe tube components to facilitate controlled fluid flow in assay cartridges are provided herein. Such components can include a valve body and syringe tube that are integrally formed as a single component. Components can include a syringe tube having a conduit in fluid communication with a valve body having a filter cavity and set of ports to facilitate direct fluid flow between chambers and fluid flow through a filter in the filter cavity of the valve body. Valve body can include an off-center filter cavity and a direct flow path in fluid communication with a filter flow path disposed in a common plane and designed to inhibit cross-contamination between flow paths. Valve body can be sealed by a cap snap-fit or welded to the valve body or a thin film. Flow valves can also be included. Two-part welding stack improves control of filter compression and performance.
Integrated valve body and syringe tube components to facilitate controlled fluid flow in assay cartridges are provided herein. Such components can include a valve body and syringe tube that are integrally formed as a single component. Components can include a syringe tube having a conduit in fluid communication with a valve body having a filter cavity and set of ports to facilitate direct fluid flow between chambers and fluid flow through a filter in the filter cavity of the valve body. Valve body can include an off-center filter cavity and a direct flow path in fluid communication with a filter flow path disposed in a common plane and designed to inhibit cross-contamination between flow paths. Valve body can be sealed by a cap snap-fit or welded to the valve body or a thin film. Flow valves can also be included. Two-part welding stack improves control of filter compression and performance.
A semi-fluid dispensing device having an injection block with vent relief to facilitate improve dispensing of a semi-fluid, such as grease, to a component. The system can further include a metering assembly for precise metering of the semi-fluid to the component to avoid excess deposition and waste. The injection block can include multiple plates stacked together, and can include a middle injection plate having defined therein an injection conduit, injection flow channels extending around a through-hole that receives the component and a vent relief channel that allows air to exit the channels through a vent relief outlet as the semi-fluid is dispensed. The device can incorporate the in-line needle shut-off valve into an existing grease deposition equipment thereby providing compatibility with an existing footprint and assembly workflow.
Automated oligonucleotide synthesis-compatible sulforhodamine dye phosphoramidite compounds and labeled polynucleotides incorporating these dyes are provided.
A biological sample processing apparatus having an enclosure. A plurality of sample processing modules are held by the enclosure. Each sample processing module is configured to hold a removable sample cartridge and to only perform sample processing on a sample within the corresponding removable sample cartridge. Each sample processing module is configured to perform at least one of a plurality of testing processes on the sample within the removable sample cartridge. At least one module in the apparatus is configured to perform nucleic acid amplification and detection.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
60.
SYSTEMS AND METHODS FOR AI BASED INFECTION CLASSIFICATION
Methods, systems, and computer storage media may be used to detect and differentiate bacterial and viral infections. Cellular population parameters such as the monocyte distribution width (MDW) can be acquired for an individual. Clinical measurements such as a complete blood count (CBC), a measure of procalcitonin (PCT) or a measure of C-reactive protein (CRP) can be obtained for an individual. A trained model can be used to compute an output value from the parameters. The output value can be compared to a threshold value to determine a probability associated with a bacterial or viral infection. A report can be generated from the probability and sent to the appropriate party.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 10/60 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données spécifiques de patients, p. ex. pour des dossiers électroniques de patients
G16H 15/00 - TIC spécialement adaptées aux rapports médicaux, p. ex. leur création ou leur transmission
G16H 20/00 - TIC spécialement adaptées aux thérapies ou aux plans d’amélioration de la santé, p. ex. pour manier les prescriptions, orienter la thérapie ou surveiller l’observance par les patients
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p. ex. basé sur des systèmes experts médicaux
G16H 50/30 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le calcul des indices de santéTIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour l’évaluation des risques pour la santé d’une personne
61.
SYSTEMS AND METHODS FOR AI BASED INFECTION CLASSIFICATION
Methods, systems, and computer storage media may be used to detect and differentiate bacterial and viral infections. Cellular population parameters such as the monocyte distribution width (MDW) can be acquired for an individual. Clinical measurements such as a complete blood count (CBC), a measure of procalcitonin (PCT) or a measure of C-reactive protein (CRP)can be obtained for an individual. A trained model can be used to compute an output value from the parameters. The output value can be compared to a threshold value to determine a probability associated with a bacterial or viral infection. A report can be generated from the probability and sent to the appropriate party.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 10/60 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données spécifiques de patients, p. ex. pour des dossiers électroniques de patients
G16H 15/00 - TIC spécialement adaptées aux rapports médicaux, p. ex. leur création ou leur transmission
G16H 20/00 - TIC spécialement adaptées aux thérapies ou aux plans d’amélioration de la santé, p. ex. pour manier les prescriptions, orienter la thérapie ou surveiller l’observance par les patients
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p. ex. basé sur des systèmes experts médicaux
G16H 50/30 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le calcul des indices de santéTIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour l’évaluation des risques pour la santé d’une personne
A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container. The bottom-cap further includes one or more auxiliary ports for injecting a reagent when the lid apparatus is in a closed configuration sealingly attached to the multi-chambered sample container.
B65D 47/08 - Fermetures avec dispositifs de décharge autres que des pompes avec goulottes ou tubes de déversementFermetures avec dispositifs de décharge autres que des pompes avec tuyères ou passages pour la décharge ayant des fermetures articulées ou à charnières
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
The present disclosure provides pancoronavirus biomarker panels that can be used to detect a wide variety of characterized coronavirus strains and, in some embodiments, emerging or novel coronavirus strains. In certain embodiments, this detection can be carried out in a single, highly-multiplexed nucleic acid amplification assay.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Thermal control devices and methods to provide improved control, speed and accuracy in temperature cycling of a fluid. Such devices and methods measure or estimate temperature of fluid non-invasively and in real-time by use of capacitance measurements of the fluid. Such systems include use of multiple electrodes positioned along the reaction-vessel, such that capacitance of the fluid is determined without contact, thereby preventing contamination of the fluid or sensors. The temperature or changes in temperature can be estimated based on capacitance in combination with a known or assumed relationship between temperature and the dielectric constant of the fluid. Optionally, the capacitance-based estimate can be improved by algorithmically combining with a measurement(s) from additional temperature sensor(s) in proximity to the reaction-vessel. Such systems and methods can be used for thermal cycling in biological processing and testing, as well as any industrial and chemical processes that can employ non-invasive fluid temperature monitoring.
The present disclosure provides a set of primers and optional probes for identifying the presence of α-coronavirus, β-coronavirus, SARS-COV-2, adenovirus, Chlamydia pneumoniae, Influenza A, Influenza B, metapneumovirus, rhinovirus/enterovirus, Mycoplasma, Bordetella spp., parainfluenza, and respiratory syncytial virus (RSV), which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
66.
Encoderless motor with improved quantization and methods of use and calibration
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor to be determined from the sinusoidal signals by utilizing a transformation matrix or piece-wise algorithm applied in substantially linear portions of the sinusoidal signals without requiring use of additional hardware encoder or position sensors and without requiring noise-reduction or filtering of the signal.
A fluidic bridge device for transport of a fluid sample between a first sample processing device and a second sample processing device. The fluidic bridge may include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the first and second sample processing device. In one aspect, the first device is a sample preparation device and the second device is an assay device. The fluidic bridge can include at least two fluid conduits, at least one for transport of the prepared sample, and at least one other to facilitate displacement of air to allow flow of the prepared sample through the other fluid conduit. The fluid channels can include one or more of an amplification chamber, a processing chamber, a gas-permeable vent, a bubble trap, a filter, and an external port. Methods of preparing and transporting a fluid sample between devices are provided herein.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Described herein are methods and compositions that provide highly efficient primer annealing and denaturation during nucleic acid amplification. The basic method employs destabilizing nucleotide triphosphates (dNTPs) in amplification reaction mixtures. The incorporation of such dNTPs into the amplicon produced upon amplification lowers the Tm of the amplicon, promoting denaturation/strand separation for the next round of primer annealing.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
Described herein are methods and compositions that provide highly efficient primer annealing and denaturation during nucleic acid amplification. The basic method employs destabilizing nucleotide triphosphates (dNTPs) in amplification reaction mixtures. The incorporation of such dNTPs into the amplicon produced upon amplification lowers the Tm of the amplicon, promoting denaturation/strand separation for the next round of primer annealing.
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
The present disclosure provides a set of primers and optional probes for identifying the presence of α-coronavirus, β-coronavirus, SARS-CoV-2, adenovirus, Chlamydia pneumoniae, Influenza A, Influenza B, metapneumovirus, rhinovirus/enterovirus, mycoplasma, Bordetella spp., parainfluenza, and respiratory syncytial virus (RSV), which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
B01L 9/00 - Dispositifs de supportDispositifs de serrage
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
The present disclosure provides a set of primers and optional probes for identifying the presence of MPOX virus, Clade II, which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
The present disclosure provides a set of primers and optional probes for identifying the presence of MPOX virus, Clade II, which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
Methods and systems for detecting defect in sample cartridges in real-time during manufacturing. Such systems utilize one or more external sensors that detect characteristics or parameters of the sample cartridge and/or the manufacturing process from one or more data sets. The external sensor(s) include any of: an RGB camera, IR camera, high-resolution optical camera, and ultrasonic microphone or combination thereof. An automated system obtains data sets from external sensor(s) and compares the data sets to a baseline of the sample cartridge and/or manufacturing process such that defects can be determined based on a variance from the baseline. Such methods can utilize feature extraction and spectrum analysis to identify features or characteristics for comparison with the baseline. A machine learning model can be used to determine an algorithm based on data sets of acceptable sample cartridges and data sets from the external sensor(s) that are associated with the cartridge defect.
B23K 20/10 - Soudage non électrique par percussion ou par une autre forme de pression, avec ou sans chauffage, p. ex. revêtement ou placage utilisant des vibrations, p. ex. soudage ultrasonique
G01N 21/90 - Recherche de la présence de criques, de défauts ou de souillures dans un récipient ou dans son contenu
Methods and system for identifying faulty sample cartridges in real-time during manufacturing are provided herein. Such systems use monitored operational parameters associated with operation of manufacturing equipment that manufactures the sample cartridges. One or more data sets of operational parameters are obtained from existing equipment controls and/or additional sensors and compared to corresponding operational parameters associated with acceptable cartridges. A faulty detection unit can be configured to compare the operational parameters and can optionally include algorithms and/or models, such as a machine learning model, by which faulty cartridges can be identified. The comparison can include determining whether the monitored operational parameters are within a pre-defined range of acceptable values and/or deviate from a characteristic profile of the operational parameter in a manner indicative of faulty cartridges. The faulty cartridge detection can be integrated within automation controls so that faulty cartridges can be detected in real-time and automatically discarded during manufacturing.
Methods and systems for training a model for automated defect detection of a product during manufacturing are provided herein. Such methods utilize a combination of supervised transfer learning through auxiliary tasks and a combination of supervised and unsupervised learning. The methods can utilize supervised transfer learning with expert labels on a generalized auxiliary task, such as product classification, which is transferred to more specific auxiliary tasks, such as identification of specific product features and/or anomaly detection, where additional expert labels are then applied to the anomalies, and another iteration of supervised learning further improves the model. The anomalies can correspond to features associated with defects, which can be induced experimentally to improve efficiency of the training procedure. The product can be a sample cartridge such that the model allows detection of faulty cartridges based on sample cartridge and/or manufacturing process data.
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with a first set of permanent magnets distributed radially about the rotor to facilitate rotation of the rotor and a second set of permanent magnets on the rotor to facilitate determination of an absolute position of the rotor. The motor further includes first and second set of sensors for detection of the magnets of the inner and outer rings. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an absolute position of the rotor relative the substrate to be determined from the sinusoidal signals without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02P 7/025 - Dispositions pour réguler ou commander la vitesse ou le couple de moteurs électriques à courant continu les moteurs à courant continu étant du type linéaire les moteurs à courant continu étant du type à bobine mobile, p. ex. moteurs de bobine acoustique
H02K 1/2791 - Aimants montés en surfaceAimants sertis
H02K 1/2795 - Rotors faisant face axialement à des stators le rotor étant formé de plusieurs aimants disposés sur la circonférence
81.
Film seal assembly and single lid assay cartridge and associated methods
Film seal assemblies that seal one or more reagents within a sample cartridge, which allow for single lid cartridge designs. Such film seal assemblies can include one or more layers, a bottom-most layer that permanently seals with a sample cartridge having multiple chambers for one or more reagents, and top-most layer that releasably seals vent opening(s) in the film seal assembly and is removable by the end user. This configuration allows use of a single lid, which can be integrally formed with cartridge body or a separate component attached atop the cartridge body. The film seal assembly can include peelable liner(s) or can releasably attached to an underside of the single lid. Such film assemblies can include multiple layers that include a polypropylene layer for permanently sealing to a rigid propylene cartridge body and polyester top layers for releasably and hermetically sealing with a liner or the lid.
INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
84.
FILM SEAL ASSEMBLY AND SINGLE LID ASSAY CARTRIDGE AND ASSOCIATED METHODS
Film seal assemblies that seal one or more reagents within a sample cartridge, which allow for single lid cartridge designs. Such film seal assemblies can include one or more layers, a bottom-most layer that permanently seals with a sample cartridge having multiple chambers for one or more reagents, and top-most layer that releasably seals vent opening(s) in the film seal assembly and is removable by the end user. This configuration allows use of a single lid, which can be integrally formed with cartridge body or a separate component attached atop the cartridge body. The film seal assembly can include peelable liner(s) or can releasably attached to an underside of the single lid. Such film assemblies can include multiple layers that include a polypropylene layer for permanently sealing to a rigid propylene cartridge body and polyester top layers for releasably and hermetically sealing with a liner or the lid.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B65D 17/50 - Éléments frangibles rapportés, appliqués à, ou logés dans, des ouvertures préformées, p. ex. bandes à déchirer ou bouchons plastiques
B65D 51/16 - Fermetures non prévues ailleurs avec moyens pour laisser partir l'air ou le gaz
B65D 51/22 - Capuchons, couvercles ou chapeaux fonctionnant avec une fermeture intérieure disposée pour être ouverte en perçant, coupant ou déchirant comportant des moyens pour percer, couper ou déchirer la fermeture intérieure
B65D 53/08 - Bandes flexibles adhésives conçues pour sceller les ouvertures de remplissage ou d'évacuation
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. The method employs pairs of primers that differ significantly in Tm and a novel temperature/time course characterized by a temperature pulse during denaturation that enables a high-Tm primer (but not a low-Tm primer) to anneal and prime the synthesis of an additional nucleic acid strand beyond the two strands synthesized in a cycle of classical PCR. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
Methods and systems for detecting defect in sample cartridges in real-time during manufacturing. Such systems utilize one or more external sensors that detect characteristics or parameters of the sample cartridge and/or the manufacturing process from one or more data sets. The external sensor(s) include any of: an RGB camera, IR camera, high-resolution optical camera, and ultrasonic microphone or combination thereof. An automated system obtains data sets from external sensor(s) and compares the data sets to a baseline of the sample cartridge and/or manufacturing process such that defects can be determined based on a variance from the baseline. Such methods can utilize feature extraction and spectrum analysis to identify features or characteristics for comparison with the baseline. A machine learning model can be used to determine an algorithm based on data sets of acceptable sample cartridges and data sets from the external sensor(s) that are associated with the cartridge defect.
Methods and system for identifying faulty sample cartridges in real-time during manufacturing are provided herein. Such systems use monitored operational parameters associated with operation of manufacturing equipment that manufactures the sample cartridges. One or more data sets of operational parameters are obtained from existing equipment controls and/or additional sensors and compared to corresponding operational parameters associated with acceptable cartridges. A faulty detection unit can be configured to compare the operational parameters and can optionally include algorithms and/or models, such as a machine learning model, by which faulty cartridges can be identified. The comparison can include determining whether the monitored operational parameters are within a pre-defined range of acceptable values and/or deviate from a characteristic profile of the operational parameter in a manner indicative of faulty cartridges. The faulty cartridge detection can be integrated within automation controls so that faulty cartridges can be detected in real-time and automatically discarded during manufacturing.
Methods and systems for training a model for automated defect detection of a product during manufacturing are provided herein. Such methods utilize a combination of supervised transfer learning through auxiliary tasks and a combination of supervised and unsupervised learning. The methods can utilize supervised transfer learning with expert labels on a generalized auxiliary task, such as product classification, which is transferred to more specific auxiliary tasks, such as identification of specific product features and/or anomaly detection, where additional expert labels are then applied to the anomalies, and another iteration of supervised learning further improves the model. The anomalies can correspond to features associated with defects, which can be induced experimentally to improve efficiency of the training procedure. The product can be a sample cartridge such that the model allows detection of faulty cartridges based on sample cartridge and/or manufacturing process data.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.
Automated oligonucleotide synthesis-compatible sulforhodamine dye phosphoramidite compounds and labeled polynucleotides incorporating these dyes are provided.
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a modified solid support comprising an amine or amide group.
Systems for processing a fluid sample to facilitate analysis with a semiconductor detection chip are provided herein. Such systems can include a sample processing cartridge coupleable with a chip carrier device configured for transport of the processed fluid sample from the sample cartridge. The chip carrier device can include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the sample processing cartridge. The chip carrier device can include multiple portions or adapters, including a fluid sample portion, a flowcell portion and a chip carrier. Also provided are methods of preparing and transporting a fluid sample from a sample cartridge into a chip carrier device for analysis with a semiconductor detection chip carried within the chip carrier device.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
Test cartridges for performing multi-target assay panels, in particular multiplex vaginal panels (MVP) and methods of use and manufacture. Such cartridges can include a cartridge body having multiple chambers and rotatable valve assembly to facilitate controlled fluid flow, and various reagents and buffers to chemically lyse nucleic acids from multiple targets in MVP. Specialized valve assemblies have been utilized to resist alkaline lysing buffers required to chemically lyse multiple targets, including various protozoa, yeasts and bacteria, including Atopobium spp. (Atopobium vaginae, Atopobium novel species CCUG 55226); Bacterial Vaginosis-Associated Bacterium 2 (BVAB2); and Megasphaera-1. Methods include detecting levels of each respective target in a single sample from a single multi-target assay cartridge and identifying the subject as having bacterial vaginosis, vulvovaginal candidiasis (or the presence of Candida spp.), or trichomoniasis (or presence of Trichomonas vaginalis) based on detected levels of each of: Atopobium spp., BVAB2, Megasphaera-1, Candida spp., and Trichomonas vaginalis.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/6893 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les protozoaires
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
95.
Encoderless motor with improved granularity and methods of use
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor relative the substrate to be determined from linear portions of the sinusoidal signal without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02P 6/16 - Dispositions de circuits pour détecter la position
H02K 1/27 - Noyaux rotoriques à aimants permanents
H02K 1/2791 - Aimants montés en surfaceAimants sertis
H02K 11/00 - Association structurelle de machines dynamo-électriques à des organes électriques ou à des dispositifs de blindage, de surveillance ou de protection
H02K 11/215 - Dispositifs utilisant un effet magnétique, p. ex. des éléments à effet Hall ou magnéto-résistifs
H02K 15/02 - Procédés ou appareils spécialement adaptés à la fabrication, l'assemblage, l'entretien ou la réparation des machines dynamo-électriques des corps statoriques ou rotoriques
H02K 29/08 - Moteurs ou génératrices à dispositifs de commutation non mécaniques, p. ex. tubes à décharge ou dispositifs à semi-conducteurs avec des dispositifs détecteurs de la position utilisant des dispositifs à effet magnétique, p. ex. dispositifs à effet Hall ou magnéto-résistances
H02K 21/22 - Moteurs synchrones à aimants permanentsGénératrices synchrones à aimants permanents avec des induits fixes et des aimants tournants avec des aimants tournant autour des induits, p. ex. volants magnétiques
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
97.
NUCLEIC ACID EXTRACTION AND ISOLATION WITH HEAT LABILE SILANES AND CHEMICALLY MODIFIED SOLID SUPPORTS
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a modified solid support comprising an amine or amide group.
Improved sample cartridges, valve assemblies and methods of manufacture and assembly are provided herein. Such systems can include a sample processing cartridge having a unitary cartridge body with integral syringe tube and valve interface. Such systems can further includes valve assemblies with an overmolded gasket and gaskets with a protruding conical valve sealing surface. Various additional features can include thin film sealing for cartridge as well as valve assemblies for chemical lysis. Thin film sealing for cartridge lids can include various multi-layered designs to facilitate injection and sealing of reagents within the cartridge. Magnetic separation features are also included. Such features can be included in various design iterations as needed for compatibility with existing technologies and to accommodate needs for manufacturing workflows.
Diagnostic detection chip device designs that reduce cost of fabrication and assembly are described herein. Such chip device designs include features that facilitate use of the chip within a chip carrier device with integrated fluid flow control features and compatibility with conventional sample cartridges and sample processing systems. Associated methods of manufacture and assembly of the chip devices are also provided herein.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
Improved sample cartridges, valve assemblies and methods of manufacture and assembly are provided herein. Such systems can include a sample processing cartridge having a unitary cartridge body with integral syringe tube and valve interface. Such systems can further includes valve assemblies with an overmolded gasket and gaskets with a protruding conical valve sealing surface. Various additional features can include thin film sealing for cartridge as well as valve assemblies for chemical lysis. Thin film sealing for cartridge lids can include various multi-layered designs to facilitate injection and sealing of reagents within the cartridge. Magnetic separation features are also included. Such features can be included in various design iterations as needed for compatibility with existing technologies and to accommodate needs for manufacturing workflows.
