Abrégé

The invention provides a fluorosurfactant composition for use in stabilising emulsions, in particular emulsions comprising single or double-emulsion droplets; as well as improved methods for manufacture of the fluorosurfactant; wherein the surfactant comprises a perfluorocarbon chain amide covalently linked to a polymer of ethylene 5 oxide and propylene oxide. The invention also provides methods for single-cell resolution cell killing- and cell secretion-assays based on droplets stabilised by the fluorosurfactant composition.

Classes IPC  ?

• C08G 65/00 - Composés macromoléculaires obtenus par des réactions créant une liaison éther dans la chaîne principale de la macromolécule
• C09K 23/00 - Utilisation de substances comme agents émulsifiants, humidifiants, dispersants ou générateurs de mousse
• G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique

Abrégé

The present invention relates to a system for sorting particles by characteristics that can be optically detected. The system comprises a microfluidic device that is capable of sorting the particles, and an instrument driving the fluids through the microfluidic device and invoking the sorting events in response to the optical signals emitted by the particles. The present invention also relates to a method for enrichment or isolation of a nucleotide fragment comprising a known nucleotide sequence element and to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for sorting of emulsion droplets.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

Provides a method to obtain enrichment of large DNA molecules for long-read sequencing using FACS or microfluidic partitioning. Furthermore, the present invention relates to a kit comprising a plurality of 5 microfluidic devices and a plurality of fluids configured for use with the system and the method.

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]

Abrégé

The invention provides a fluorosurfactant composition for use in stabilising emulsions, in particular emulsions comprising single or double-emulsion droplets; as well as improved methods for manufacture of the fluorosurfactant; wherein the surfactant comprises a perfluorocarbon chain amide covalently linked to a polymer of ethylene 5 oxide and propylene oxide. The invention also provides methods for single-cell resolution cell killing- and cell secretion-assays based on droplets stabilised by the fluorosurfactant composition.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• C08G 65/00 - Composés macromoléculaires obtenus par des réactions créant une liaison éther dans la chaîne principale de la macromolécule
• C08G 65/26 - Composés macromoléculaires obtenus par des réactions créant une liaison éther dans la chaîne principale de la macromolécule à partir d'éthers cycliques par ouverture d'un hétérocycle à partir d'éthers cycliques et d'autres composés
• C08G 65/332 - Polymères modifiés par post-traitement chimique avec des composés organiques contenant de l'oxygène contenant des groupes carboxyle, ou leurs halogénures ou esters
• C08G 65/337 - Polymères modifiés par post-traitement chimique avec des composés organiques contenant d'autres éléments
• C08L 71/00 - Compositions contenant des polyéthers obtenus par des réactions créant une liaison éther dans la chaîne principaleCompositions contenant des dérivés de tels polymères
• C08L 71/02 - Oxydes des polyalkylènes
• C09K 23/00 - Utilisation de substances comme agents émulsifiants, humidifiants, dispersants ou générateurs de mousse
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]

Abrégé

The present invention relates to a system for amplification of polynucleotides from a predefined number of single cells. The system comprise a device (or part) providing the predefined number of single cells, at a previously defined inlet site (or orifice) of a cartridge (microfluidic device), and the cartridge itself. The invention further relates to a method for amplification of polynucleotides from the one or more single cells using the system to provide an emulsion of aqueous droplets wherein the nucleic acid amplification occurs. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the system and the method.

Classes IPC  ?

• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• B01L 3/02 - BurettesPipettes

Abrégé

The present invention relates to a system for sorting particles by characteristics that can be optically detected. The system comprises a microfluidic device that is capable of sorting the particles, and an instrument driving the fluids through the microfluidic device and invoking the sorting events in response to the optical signals emitted by the particles. The present invention also relates to a method for enrichment or isolation of a nucleotide fragment comprising a known nucleotide sequence element and to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for sorting of emulsion droplets.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

A microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

A microfluidic device (100) comprises an emulsification section (101) comprising one or more emulsification units (170); and a container section (102) comprising one or more groups of containers comprising one group of containers for each emulsification unit; each emulsification unit (170) comprising a fluid conduit network (135) comprising: a plurality of supply conduits comprising a primary supply conduit (103) and a secondary supply conduit (106); a transfer conduit (112); and a first fluid junction (120) providing fluid communication between the primary supply conduit (103), the secondary supply conduit (106), and the transfer conduit (112); each group of containers (103) comprising a plurality of containers comprising an intermediate chamber (174), a collection container (134), and one or more supply containers (131) comprising a secondary supply container, the secondary supply container (131) defining a secondary supply cavity, the secondary supply container (131) comprising a secondary orifice (177) extending from the secondary supply cavity and a primary orifice (176) extending from the secondary supply cavity, the collection container (134) being in fluid communication with the transfer conduit (112) of the corresponding emulsification unit (170) via a collection orifice of the collection container (134), the secondary supply container (131) being in fluid communication with the secondary supply conduit (106) of the corresponding emulsification unit (170) via the secondary orifice (177), the secondary supply container (131) being in fluid communication with the intermediate chamber (174) of the same group of containers (103) via the primary orifice (176), the intermediate chamber (174) being in fluid communication with the first fluid junction (120) of the corresponding emulsification unit (170) via the primary supply conduit (103) of the corresponding emulsification unit (170). Furthermore a method of manufacturing said device and a method for providing emulsion droplets using such a microfluidic device.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

The present invention relates to a microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, the present invention relates to an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets. The microfluidic device comprises a transfer conduit comprising a first transfer conduit part having a first affinity for water; and a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water. A well section and a microfluidic section of the microfluidic device are fixedly connected to each other.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• B01F 23/41 - Émulsion
• B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central
• B01F 33/301 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux
• B01F 33/30 - Micromixeurs
• B01F 101/23 - Mélange d'échantillons de laboratoire, p. ex. en vue d'analyser ou de tester les propriétés des matières

Abrégé

The present invention relates to a system for amplification of polynucleotides from a predefined number of single cells. The system comprise a device (or part) providing the predefined number of single cells, at a previously defined inlet site (or orifice) of a cartridge (microfluidic device), and the cartridge itself. The invention further relates to a method for amplification of polynucleotides from the one or more single cells using the system to provide an emulsion of aqueous droplets wherein the nucleic acid amplification occurs. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the system and the method.

Classes IPC  ?

• B01L 3/02 - BurettesPipettes
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• B01F 3/08 - Mélange, p.ex. dispersion, émulsion, selon les phases à mélanger de liquides avec des liquides; Emulsion
• B01F 13/00 - Autres mélangeurs; Installations pour effectuer des mélanges comportant des combinaisons de mélangeurs de types différents
• B01F 13/10 - Installations pour effectuer des mélanges comprenant des combinaisons de mélangeurs de types différents

Abrégé

The present invention pertains to an in vitro method in which a targeted DNA molecule containing a DNA sequence of interest is enriched by a) general amplification of DNA molecules in a multiple of droplets each containing less than 0.5 target DNA molecule on average (404), b) specific detection of the target DNA molecule in each of the droplets (405), and c) physically selecting droplets containing target DNA molecules (406).

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

The present invention relates to a microfluidic device and method for providing emulsion droplets. The device comprising: a microfluidic section comprising one or more microfluidic units; and a well section comprising one or more groups of wells comprising one group of wells for each microfluidic unit; the well section and the microfluidic section forming a fixedly connected unit such that each group of wells forms a fixedly connected unit with a respective corresponding microfluidic unit, each microfluidic unit comprising a fluid conduit network comprising: a plurality of supply conduits comprising a secondary supply conduit and a primary supply conduit comprising a capillary structure having a volume of at least 2 μL; a transfer conduit; and a first fluid junction providing fluid communication between the primary supply conduit, the secondary supply conduit, and the transfer conduit; each group of wells comprising a plurality of wells comprising a collection well and one or more supply wells comprising a primary supply well, the collection well being in fluid communication with the transfer conduit of the corresponding microfluidic unit, the primary supply well being in fluid communication with the primary supply conduit and the secondary supply conduit of the corresponding microfluidic unit.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• B01F 23/41 - Émulsion
• B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central

Abrégé

The present disclosure relates to methods for enrichment or isolation of a long nucleotide fragment comprising a known nucleotide sequence element, i.e. a sequence encoding a conserved active site or domain, the method being applicable i.a. to high throughput screening for DNA fragments containing a known sequence element. The methods herein comprise the steps of forming an emulsion of multiple liquid droplets from a DNA fragment containing liquid sample, specific detection of droplets containing at least one target DNA molecule, physically selecting droplets containing at least one target DNA molecule, and performing a general amplification of the DNA molecules containing at least one target DNA molecules.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

A microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets.

Classes IPC  ?

• B01F 23/41 - Émulsion
• B01F 35/71 - Mécanismes d'alimentation

Abrégé

A microfluidic device (100) comprises an emulsification section (101) comprising one or more emulsification units (170); and a container section (102) comprising one or more groups of containers comprising one group of containers for each emulsification unit; each emulsification unit (170) comprising a fluid conduit network (135) comprising: a plurality of supply conduits comprising a primary supply conduit (103) and a secondary supply conduit (106); a transfer conduit (112); and a first fluid junction (120) providing fluid communication between the primary supply conduit (103), the secondary supply conduit (106), and the transfer conduit (112); each group of containers (103) comprising a plurality of containers comprising an intermediate chamber (174), a collection container (134), and one or more supply containers (131) comprising a secondary supply container, the secondary supply container (131) defining a secondary supply cavity, the secondary supply container (131) comprising a secondary orifice (177) extending from the secondary supply cavity and a primary orifice (176) extending from the secondary supply cavity, the collection container (134) being in fluid communication with the transfer conduit (112) of the corresponding emulsification unit (170) via a collection orifice of the collection container (134), the secondary supply container (131) being in fluid communication with the secondary supply conduit (106) of the corresponding emulsification unit (170) via the secondary orifice (177), the secondary supply container (131) being in fluid communication with the intermediate chamber (174) of the same group of containers (103) via the primary orifice (176), the intermediate chamber (174) being in fluid communication with the first fluid junction (120) of the corresponding emulsification unit (170) via the primary supply conduit (103) of the corresponding emulsification unit (170). Furthermore a method of manufacturing said device and a method for providing emulsion droplets using such a microfluidic device.

Classes IPC  ?

• B01F 23/41 - Émulsion
• B01F 35/71 - Mécanismes d'alimentation

Abrégé

A microfluidic device (100) comprises an emulsification section (101) comprising one or more emulsification units (170); and a container section (102) comprising one or more groups of containers comprising one group of containers for each emulsification unit; each emulsification unit (170) comprising a fluid conduit network (135) comprising: a plurality of supply conduits comprising a primary supply conduit (103) and a secondary supply conduit (106); a transfer conduit (112); and a first fluid junction (120) providing fluid communication between the primary supply conduit (103), the secondary supply conduit (106), and the transfer conduit (112); each group of containers (103) comprising a plurality of containers comprising an intermediate chamber (174), a collection container (134), and one or more supply containers (131) comprising a secondary supply container, the secondary supply container (131) defining a secondary supply cavity, the secondary supply container (131) comprising a secondary orifice (177) extending from the secondary supply cavity and a primary orifice (176) extending from the secondary supply cavity, the collection container (134) being in fluid communication with the transfer conduit (112) of the corresponding emulsification unit (170) via a collection orifice of the collection container (134), the secondary supply container (131) being in fluid communication with the secondary supply conduit (106) of the corresponding emulsification unit (170) via the secondary orifice (177), the secondary supply container (131) being in fluid communication with the intermediate chamber (174) of the same group of containers (103) via the primary orifice (176), the intermediate chamber (174) being in fluid communication with the first fluid junction (120) of the corresponding emulsification unit (170) via the primary supply conduit (103) of the corresponding emulsification unit (170). Furthermore a method of manufacturing said device and a method for providing emulsion droplets using such a microfluidic device.

Classes IPC  ?

• B01F 3/08 - Mélange, p.ex. dispersion, émulsion, selon les phases à mélanger de liquides avec des liquides; Emulsion
• B01F 13/00 - Autres mélangeurs; Installations pour effectuer des mélanges comportant des combinaisons de mélangeurs de types différents
• B01F 13/10 - Installations pour effectuer des mélanges comprenant des combinaisons de mélangeurs de types différents

Abrégé

A microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets.

Classes IPC  ?

• B01F 3/08 - Mélange, p.ex. dispersion, émulsion, selon les phases à mélanger de liquides avec des liquides; Emulsion
• B01F 13/00 - Autres mélangeurs; Installations pour effectuer des mélanges comportant des combinaisons de mélangeurs de types différents
• B01F 13/10 - Installations pour effectuer des mélanges comprenant des combinaisons de mélangeurs de types différents

Abrégé

The present invention pertains to an in vitro method in which the frequency of the targeted nucleotide sequence containing the DNA fragment of interest is increased stepwise, by several rounds of 1) dilution of a sample containing the DNA fragment of interest into several replicates (separation), 2) randomly amplifying DNA in the replicates (concentration), 3) detecting the DNA fragment of interest in at least one of the diluted and amplified replicates (selection) and repeating steps 1) through 3) until the DNA fragment of interest can be sequenced by standard sequencing techniques.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Produits et services

(Based on 44(e)) Chemical preparations and reagents for use in droplet-based single molecule handling; reagents in kit form for use in droplet- based single molecule handling composed of emulsion-forming chemicals, buffers, and reagents to amplify nucleic acid for use in the biotechnology field (Based on 44(e)) Droplet-based pharmaceutical and veterinary preparations, droplet-based sanitary preparations, droplet-based medical preparations, and droplet-based reagents for use in single molecule analysis or single molecular screening of DNA or RNA (Based on 44(e)) Instruments and cartridges for microfluidics-based and droplet-based single molecule handling; data processing equipment and computers for single molecule handling, single molecule analysis or single molecule screening of DNA or RNA (Based on Use in Commerce) Apparatus for DNA testing for medical purposes; apparatus for analyzing substances for medical use (Based on 44(e)) Scientific services and research for others in the field of DNA analysis; medical research services in the field of medical laboratory service; research services relating to health and healthcare, namely, biomedical research services; DNA screening for scientific research purposes; advisory, consultancy and information services relating to the aforesaid (Based on 44(e)) Medical services; DNA screening for medical purposes; medical and health services relating to DNA, genetics and genetic testing; advisory, consultancy and information services relating to the aforesaid

Produits et services

Chemical preparations for scientific use in analyzing nucleic acids; chemical compositions for scientific and research use in analyzing nucleic acids; materials for use in science, namely, compositions for sample preparations; reagents for scientific use in analyzing nucleic acids; reagent kits consisting primarily of emulsion-forming chemicals, buffers, and reagents to amplify nucleic acid for use in biotechnology fields Diagnostic preparations for medical purposes; reagents for medical purposes; chemical preparations for DNA analysis; none of the aforesaid relating to ophthalmic preparations Scientific apparatus and instruments, namely, droplet generators; for sample preparation of nucleic acids; apparatus for DNA testing for scientific purposes; DNA microarrays Apparatus for DNA testing for medical purposes; medical apparatus for analyzing substances, namely, samples of nucleic acids

Abrégé

The present invention relates to a microfluidic device and method for providing emulsion droplets. The device comprising: a microfluidic section comprising one or more microfluidic units; and a well section comprising one or more groups of wells comprising one group of wells for each microfluidic unit; the well section and the microfluidic section forming a fixedly connected unit such that each group of wells forms a fixedly connected unit with a respective corresponding microfluidic unit, each microfluidic unit comprising a fluid conduit network comprising: a plurality of supply conduits comprising a secondary supply conduit and a primary supply conduit comprising a capillary structure having a volume of at least 2 µL; a transfer conduit; and a first fluid junction providing fluid communication between the primary supply conduit, the secondary supply conduit, and the transfer conduit; each group of wells comprising a plurality of wells comprising a collection well and one or more supply wells comprising a primary supply well, the collection well being in fluid communication with the transfer conduit of the corresponding microfluidic unit, the primary supply well being in fluid communication with the primary supply conduit and the secondary supply conduit of the corresponding microfluidic unit.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• B01F 3/08 - Mélange, p.ex. dispersion, émulsion, selon les phases à mélanger de liquides avec des liquides; Emulsion
• B01F 13/00 - Autres mélangeurs; Installations pour effectuer des mélanges comportant des combinaisons de mélangeurs de types différents

Abrégé

The present invention relates to a microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, the present invention relates to an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets. The microfluidic device comprises a transfer conduit comprising a first transfer conduit part having a first affinity for water; and a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water. A well section and a microfluidic section of the microfluidic device are fixedly connected to each other.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• B01F 13/00 - Autres mélangeurs; Installations pour effectuer des mélanges comportant des combinaisons de mélangeurs de types différents
• B01F 13/08 - Mélangeurs magnétiques
• B01F 13/10 - Installations pour effectuer des mélanges comprenant des combinaisons de mélangeurs de types différents
• B01F 3/08 - Mélange, p.ex. dispersion, émulsion, selon les phases à mélanger de liquides avec des liquides; Emulsion

Abrégé

The invention relates to a method for enrichment or isolation of a long nucleotide fragment comprising a known nucleotide sequence element, i.e. a sequence encoding a conserved active site or domain, the method being applicable i.a. to high throughput screening for DNA fragments containing a known sequence element. The methods include, inter alia, steps of providing a liquid sample of mixed DNA molecules comprising one or more specific target DNA molecules, formation of a multiple of liquid droplets, specific detection of droplets containing at least one of the target DNA molecules, physically selecting droplets containing at least one of the target DNA molecules, and general amplification of the DNA molecules in the coalesced selected droplets.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Produits et services

Chemical preparations; chemical compositions and materials for use in science; reagents for scientific purposes; reagents in kit form. Medical preparations; diagnostic preparations and materials; reagents for medical purposes; chemical preparations for DNA analysis; none of the aforesaid relating to ophthalmic preparations. Scientific apparatus and instruments; apparatus for DNA testing for scientific purposes; DNA microarrays. Apparatus for DNA testing for medical purposes; apparatus for analysing substances for medical use.

Produits et services

Chemical preparations, reagents and reagents in kit form for use in droplet-based single molecule handling. Droplet-based pharmaceutical and veterinary preparations, droplet-based sanitary preparations, droplet-based medical preparations, and droplet-based reagents for use in single molecule analysis or single molecular screening of DNA or RNA. Instruments and cartridges for microfluidics-based and droplet-based single molecule handling; data processing equipment and computers for single molecule handling, single molecule analysis or single molecule screening of DNA or RNA. Scientific services and research; medical research services; research services relating to health and healthcare; DNA screening for scientific research purposes; advisory, consultancy and information services relating to the aforesaid. Medical services; DNA screening for medical purposes; medical and health services relating to DNA, genetics and genetic testing; advisory, consultancy and information services relating to the aforesaid.

Abrégé

The present invention pertains to an in vitro method in which a targeted DNA molecule containing a DNA sequence of interest is enriched by a) general amplification of DNA molecules in a multiple of droplets each containing less than 0.5 target DNA molecule on average (404), b) specific detection of the target DNA molecule in each of the droplets (405), and c) physically selecting droplets containing target DNA molecules (406).

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

The invention relates to a method for enrichment or isolation of a long nucleotide fragment comprising a known nucleotide sequence element, i.e. a sequence encoding a conserved active site or domain, the method being applicable i.a. to high throughput screening for DNA fragments containing a known sequence element.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

The present invention pertains to an in vitro method in which the frequency of the targeted nucleotide sequence containing the DNA fragment of interest is increased stepwise, by several rounds of 1) dilution of a sample containing the DNA fragment of interest into several replicates (separation), 2) randomly amplifying DNA in the replicates (concentration), 3) detecting the DNA fragment of interest in at least one of the diluted and amplified replicates (selection) and repeating steps 1) through 3) until the DNA fragment of interest can be sequenced by standard sequencing techniques.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

The present invention pertains to an in vitro method in which a targeted DNA molecule containing a DNA sequence of interest is enriched by a) general amplification of DNA molecules in a multiple of droplets each containing less than 0.5 target DNA molecule on average(404), b) specific detection of the target DNA molecule in each of the droplets(405), and c) physically selecting droplets containing target DNA molecules (406).

Classes IPC  ?

• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/6844 - Réactions d’amplification d’acides nucléiques
• C12Q 1/6869 - Méthodes de séquençage

Abrégé

The present invention pertains to an in vitro method in which the frequency of the targeted nucleotide sequence containing the DNA fragment of interest is increased stepwise, by several rounds of 1) dilution of a sample containing the DNA fragment of interest into several replicates (separation), 2) randomly amplifying DNA in the replicates (concentration), 3) detecting the DNA fragment of interest in at least one of the diluted and amplified replicates (selection) and repeating steps 1) through 3) until the DNA fragment of interest can be sequenced by standard sequencing techniques.

Classes IPC  ?

• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/6844 - Réactions d’amplification d’acides nucléiques
• C12Q 1/6869 - Méthodes de séquençage