A porous polymeric membrane which comprises a porous membrane having an average pore size between about 0.001 and 10.0 microns formed of a first polymer, said substrate having a surface which is modified on its surface with a cross-linked second polymer formed from a polymerizable fluorine containing monomer that contains continuous chain of 5 carbon atoms or less with fluorine atoms, said monomer being polymerized and crosslinked on said membrane, said membrane contains less than 25 ppb of C6 PFCA (Perfluorocarboxylic acid), less than 25 ppb of C8 PFCA and less than 25 ppb of combined C9-14 PFCA.
A probe (30) for a trans-epithelial electrical resistance and trans-epithelial potential difference instrument (10) may include a temperature sensor configured to record the temperature in a well of a cell culture plate while collecting a TEER or TEPD measurement. The temperature sensor may be surface mounted on one of the legs (36) of the probe, may be disposed within a tube disposed proximate to one of the legs of the probe, or, when the probe legs are tubular, may be disposed within the conduit of one of the legs. The apical and basolateral legs may be formed as printed circuit board assemblies, where the sensing elements are subjected to an electroless nickel, immersion gold treatment process to coat the sensing elements in a layer or nickel and then a layer of gold. The apical and basolateral legs may also be formed from 304 stainless steel and may be tubular in shape.
A hollow shaft impeller (100) having a hollow impeller housing (120), a magnet (108), an impeller cap (102), an impeller retainer (106), and a plurality of impeller blades (134). The impeller housing has a hub (130) that defines an interior volume (128) and an impeller bore that provides access to the interior volume. The magnet may be sized to be disposed within the interior volume. The impeller cap may be removably coupled to the hollow impeller housing proximate to the impeller bore. The impeller retainer may be removably coupled to the magnet and sized to be disposed within the interior volume. The plurality of impeller blades may project from the hub. Optionally, fins (140) may be disposed on each of the plurality of impeller blades.
B01F 27/17 - Agitateurs avec des éléments supplémentaires montés sur l'agitateur, à des fins autres que le mélange
B01F 33/453 - Mélangeurs magnétiques; Mélangeurs avec agitateurs à entraînement magnétique en utilisant des éléments d'agitation supportés ou suspendus
4.
System And Method For Photopolymerizing Electrophoretic Gels
An acrylamide gel formulation for use in electrophoresis, and a system and method for electrophoretic gel casting. In some embodiments, the system comprises a UV-Vis curing light source, and an acrylamide formulation that allows a resolving gel and a stacking gel to be photopolymerized in a single step. In some embodiments, the resolving solution formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP) as a water-soluble photoinitiator, which allows the solution to be photopolymerized using UV radiation within a certain wavelength. In some embodiments, a dilution buffer formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethyl-benzoyl-phosphinate (LAP). The dilution buffer may be added to the resolving solution in a selected amount in order to modify the concentration of the acrylamide to a desired concentration.
C08L 33/26 - Homopolymères ou copolymères de l'acrylamide ou de la méthacrylamide
C08J 9/28 - Mise en œuvre de substances macromoléculaires pour produire des matériaux ou objets poreux ou alvéolaires; Leur post-traitement par élimination d'une phase liquide d'un objet ou d'une composition macromoléculaire, p.ex. par séchage du coagulum
A device and related method for laboratory workflow handling is disclosed. The device may include one or more processors configured to provide a plurality of actions for selection by a user. A user can select an action of the plurality of actions and the device can determine an identification corresponding to a sample. The device can embed within a two-dimensional barcode the identification and workflow data corresponding to the sample and print a label with the two-dimensional barcode on an adhesive material using a printer.
G16H 70/40 - TIC spécialement adaptées au maniement ou au traitement de références médicales concernant des médicaments, p.ex. leurs effets secondaires ou leur usage prévu
6.
FILTRATION DEVICE AND PACKAGING CONFIGURED FOR STERILIZATION
A system for sterilizing or bioburden-reducing a filter device includes a breathable microbial barrier, a filter device, and a tubing arrangement. The tubing arrangement may be coupled to at least one opening of the filter device. The tubing arrangement may include an aseptic connector, a sealable component, and a port, all of which are in fluid communication with one another. The sealable component may be disposed in the tubing arrangement between the at least one opening of the filter device and the aseptic connector. The sealable component may be reconfigurable between an open configuration, which facilitates the entry of a sterilization vapor into the tubing arrangement and the filter device when subjected to a sterilization or bioburden reduction process, and a closed configuration, where the tubing arrangement is sealed from a surrounding environment. The breathable microbial barrier may at least partially enclose the port of the tubing arrangement.
Methods of calibrating a pH sensor fixed within an enclosed vessel are disclosed. The methods include introducing a buffer into the enclosed vessel, introducing a gas mixture comprising CO2 into the enclosed vessel, measuring a pH signal of the solution, measuring a CO2 concentration of a headspace gas of the solution, and calculating a pH value with a buffer calibration curve. The methods include calculating a calibration parameter with a sensor calibration curve and calibrating the pH sensor with the calibration parameter. Reactor systems are also disclosed. The systems include an enclosed reactor, a pH sensor, a CO2 sensor, a temperature control subsystem, and a controller. Methods of facilitating pH sensor calibration without sampling in a bioreactor system are also disclosed. The methods include providing a controller and providing instructions to operably connect the controller to the pH sensor and the CO2 sensor.
Filter elements for perfusion systems and methods are provided. A filter element sheet includes a microporous membrane having a mean pore size of at least about 0.65 μm and a feed spacer comprising woven fibers and having an open area of at least about 35%. The filter element sheet can be arranged within a filter element, for example, in a spiral-wound format or in a cassette format. A perfusion system includes at least one filter element and a pump configured to control flow of a liquid feed through the at least one filter element.
A funnel-less filtration device that attaches directly to a storage container such as a cell culture media bottle. The device includes a filter collar containing one or more membranes, an inlet with a coupling device for attaching the filter device to a supply of liquid to be filtered, an outlet at a lower portion of the collar, a vacuum port in the collar below the membrane(s) and a filtered vent in the collar above the membrane(s).
Tangential flow filter including first and second endcaps, one having a feed inlet and retentate and permeate outlets, and one or more pairs of plates between the endcaps. Each plate has a respective membrane bonded to each side. A first series of openings are formed through a first plate; a second series of openings are formed through a second plate; a third series of openings are formed through the first plate; and a fourth series of openings are formed through the second plate and communicate with the third series of openings. The third and fourth series of openings are positioned in respective permeate channels in the respective regions of one side of the first plate and one side of the second plate that are devoid of filtration media. The permeate channel is in fluid communication with the permeate outlet.
A filtration system, having a filtration unit comprising a housing with a sample inlet, a sample outlet, one or more valves, a first actuator, and a second actuator and a third actuator; and a cassette adapted to fit the filtration unit, the cassette having a filter; a first reservoir in fluid communication with the filter, the first reservoir being arranged to couple to the first actuator for imparting flow to a cell-containing solution in a first direction through the filter; a second reservoir in fluid communication with the filter, the second reservoir being arranged to couple to the second actuator for imparting flow to a cell-containing solution in a second direction through said filter, wherein the second direction opposes the first direction; and a third reservoir.
A removal tool for sample well combs that is configured to aid in the extraction of a sample well comb from an electrophoresis gel. The removal tool may be either removably coupled to the sample well comb or may be permanently affixed to the sample well comb in order to provide a surface/region that is more easily graspable by a user to aid in the extraction of the sample well comb. The removal tool may include one or more engagement members that may be at least partially disposed within an opening of the sample well comb to couple the removal tool to the sample well comb. The removal tool may also include a mechanism that is configured to impart extraction forces to a surface in proximity to the sample well comb to further aid in the extraction of the sample well comb.
A bubble diverter for use within a bioreactor or perfusion system that has an open end, a closed end, and, optionally, an interior rib, wherein the bubble diverter is capable of deflecting bubbles within a bioreactor or perfusion system and methods for using the bubble diverter(s) are disclosed.
A rotary genderless valve (500) having a body (502) having a body hole (524); a body cover (510) for attaching to the body, the body cover comprising a cover hole (522) and optionally comprising alignment holes (514); a handle (504), wherein the handle moves from a closed position and an open position actuated by a rotary motion; and a rotary slide (528) attached to the handle capable of partial rotation, wherein the rotary slide has an elastomeric slide which comprises a rotary slide hole (526); and, optionally, a second genderless valve having a second rotary slide and rotary slide hole, wherein the rotary slide hole and the second rotary slide hole are in fluid communication with one another.
F16K 3/06 - Robinets-vannes ou tiroirs, c. à d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation planes; Garnitures d'étanchéité à cet effet avec éléments de fermeture articulés à pivot en forme de plaques disposées entre l'alimentation et l'évacuation
The present invention provides compact spiral-wound filter elements having cassette-like performance. The invention further provides filtration systems (e.g., TFF systems) and processes (e.g., SPTFF processes) employing compact spiral-wound filter elements having cassette-like performance.
A multi-layered flexible sparger (100) that has a bottom film layer (102), a middle film layer (106), and a top film layer (110); a first inner mesh (104) disposed between the bottom film layer and the middle film layer; a second inner mesh (114) disposed between the middle film layer and the top film layer; and a port capable of delivering a gas to the multi-layered flexible sparger disposed between the top film layer and the bottom film layer, wherein the middle film layer comprises drill holes and the top film layer comprises drill holes.
A method and a respective system for performing a chemical synthesis via a computer comprising the following steps of Defining a desired organic target molecule of the chemical synthesis to be produced in the chemical production plant; Providing information describing the desired organic target molecule to a software-based platform being hosted by a computer connected to a network, wherein the platform holds inventories of chemical suppliers and the inventories comprises of information about synthetic reagents, like by-products and/or waste, needed for the chemical synthesis; Routing the chemical synthesis via the computer, wherein the computer checks the inventories of the platform for the necessary synthetic reagents to perform the chemical synthesis to produce the target molecule by comparing the information describing the desired organic target molecule and the information in the inventories and connects those chemical suppliers to the chemical production plant which provide the most suitable synthetic reagents according to the compared information; and Producing the desired organic target molecule in the chemical production plant with the provided synthetic reagents from the chemical suppliers chosen by the computer of the software-based platform.
G16C 20/00 - Chémo-informatique, c. à d. TIC spécialement adaptées au maniement des données physicochimiques ou structurelles des particules, des éléments, des composés ou des mélanges chimiques
24.
Final Fill Assembly and Method of Integrity Testing
Apparatus and methods for redundant filtration assemblies containing filters comprising a multi-purpose vent port are disclosed, wherein the redundant filtration assemblies reduce the amount of components and overall size of the assemblies, promoting the minimization of product losses. A method(s) to conduct pre-use post-sterilization integrity test (PUPSIT) are also disclosed.
The disclosure herein relates to a method of reducing background noise during integrity testing of membrane filter to increase sensitivity and more effectively disposition failing membrane filters.
A filter plate device has an inlet and an outlet where the filter plate has a polymeric framework having a filtration zone and a membrane bed of one or more membranes bonded and sealed to the polymeric framework in said filtration zone with a thermosetting plastic, where a flow distributor is placed within the membrane bed such that fluid flows evenly through the membrane bed.
B01D 15/22 - Adsorption sélective, p.ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
An optical interface with a housing such as a bioreactor bag, and a bioreactor bag having an optical interface. In some embodiments, the optical principle of Raman technology is used to provide several embedded single-use optical interfaces in a housing, with associated Raman probe modifications if necessary. In some embodiments, the optical interface includes a single-use cap to reduce the ambient light intensity which can be detrimental to a Raman signal. The device in accordance with embodiments disclosed herein allows for the avoidance of sterility breaks that typically may occur due to the insertion into the housing of an intrusive reusable sensor through an aseptic connection.
The embodiments disclosed herein are directed to a masking device (20) for enhancing the fidelity of sample wells of a photopolymerized electrophoretic gel contained in a gel casting assembly (10) that comprises a comb (30) having a one or more teeth. The masking device (20) includes a light blocking agent fixedly located on the gel casting assembly (10) where the light blocking agent overlaps with the teeth of the comb to prevent photopolymerization of a portion of an electrophoretic gel contained between the comb and the inner surfaces of the gel casting assembly. Upon removal of the comb after electrophoretic gel photopolymerization the sample wells remain uniform.
B01D 37/02 - Revêtement préalable des éléments filtrants ou de la substance filtrante; Addition d'adjuvants de filtration au liquide à filtrer
B01D 39/18 - Autres substances filtrantes autoportantes en substance organique, p.ex. fibres synthétiques la substance étant la cellulose ou ses dérivés
A testing method that includes the steps of evacuating air from a container to a negative atmospheric pressure, the container being a collapsible, flexible container, and comprising at least two opposing flexible walls, wherein a surface of at least one of the walls internal to the container comprises a plurality of channels or recessed features on said at least one wall and monitoring a mass flow or a state of vacuum so as to determine the integrity of the container. The container can be of any size or conformation, with or without attached fittings.
G01M 3/32 - Examen de l'étanchéité des structures ou ouvrages vis-à-vis d'un fluide par utilisation d'un fluide ou en faisant le vide par mesure du taux de perte ou de gain d'un fluide, p.ex. avec des dispositifs réagissant à la pression, avec des indicateurs de débit pour récipients, p.ex. radiateurs
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
An apparatus for treating a biological fluid, comprising a plurality of filtration devices. In some embodiments, each of the plurality of filtration devices comprising at least one inlet and at least one outlet; and a first insert plate and a second insert plate opposite the first insert plate, wherein the plurality of filtration devices is disposed therebetween. In some embodiments, each filtration device has a vent port. A manifold for each of the inlets, outlets, and vent ports is optionally fluidly connected. In another embodiment, hose barb connectors or the like may be protected from damage and/or exposure by being recessed in the device or positioned at a periphery of the device. Embodiments include apparatus and methods to achieve dripless connect/disconnect and to reduce the number of sterile-to-sterile connections.
A fluid transfer device comprising: a housing; a core positioned in the housing for relative movement with the housing, the core having a bore having an inlet and an outlet spaced from the inlet, and first and second gate-receiving regions spaced from the inlet and the outlet; and an actuator operatively connected in the device to cause relative movement between said core and said housing. Also disclosed is a method of creating an aseptic connection using the fluid transfer device.
F16K 3/18 - Robinets-vannes ou tiroirs, c. à d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation planes; Garnitures d'étanchéité à cet effet avec dispositions particulières pour tenir écartées les faces d'obturation ou pour les presser l'une contre l'autre du fait du mouvement des éléments de fermeture
A61M 39/16 - Raccords ou accouplements pour tubes avec des dispositions pour la désinfection ou la stérilisation
F16K 3/26 - Robinets-vannes ou tiroirs, c. à d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation en forme de surfaces de solides de révolution avec corps de tiroir cylindrique le passage du fluide se faisant par le corps du tiroir
F16K 3/314 - Formes ou structures de la partie coulissante; Sa fixation sur sa tige
Processes and systems for filtering a liquid sample are provided. Batches of a liquid sample can be routed to two or more cycling tanks (e.g., first and second cycling tanks). Upon filling a first cycling tank, a first batch of the liquid sample can be routed to a filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the first cycling tank or to a collection vessel. Upon filling a second cycling tank, a second batch of the liquid sample is routed to the filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the second cycling tank or to the collection vessel. The filling and continuous diafiltration of batches of the liquid sample continues to alternate between the two or more cycling tanks until a total product volume is processed.
A hollow shaft impeller (100) having a hollow impeller housing (120), a magnet (108), an impeller cap (102), an impeller retainer (106), and a plurality of impeller blades (134). The impeller housing has a hub (130) that defines an interior volume (128) and an impeller bore that provides access to the interior volume. The magnet may be sized to be disposed within the interior volume. The impeller cap may be removably coupled to the hollow impeller housing proximate to the impeller bore. The impeller retainer may be removably coupled to the magnet and sized to be disposed within the interior volume. The plurality of impeller blades may project from the hub. Optionally, fins (140) may be disposed on each of the plurality of impeller blades.
B01F 33/453 - Mélangeurs magnétiques; Mélangeurs avec agitateurs à entraînement magnétique en utilisant des éléments d'agitation supportés ou suspendus
B01F 27/17 - Agitateurs avec des éléments supplémentaires montés sur l'agitateur, à des fins autres que le mélange
A hollow shaft impeller (100) having a hollow impeller housing (120), a magnet (108), an impeller cap (102), an impeller retainer (106), and a plurality of impeller blades (134). The impeller housing has a hub (130) that defines an interior volume (128) and an impeller bore that provides access to the interior volume. The magnet may be sized to be disposed within the interior volume. The impeller cap may be removably coupled to the hollow impeller housing proximate to the impeller bore. The impeller retainer may be removably coupled to the magnet and sized to be disposed within the interior volume. The plurality of impeller blades may project from the hub. Optionally, fins (140) may be disposed on each of the plurality of impeller blades.
B01F 33/453 - Mélangeurs magnétiques; Mélangeurs avec agitateurs à entraînement magnétique en utilisant des éléments d'agitation supportés ou suspendus
Port protection device particularly suited for an encapsulated filter unit. In certain embodiments, the protection device comprises a main body defining an internal region configured to receive a port or the like protruding from an encapsulated filter. In certain embodiments, the main body includes a slot that allows for easy removal of the protection device from the port or the like. A tube management system may be provided to receive and hold tubing. One suitable tube management system includes two spaced tangs that extend from the main body of the protection device and are configured to cooperatively receive and hold tubing between them.
A porous polymeric membrane which comprises a porous membrane having an average pore size between about 0.001 and 10.0 microns formed of a first polymer, said substrate having a surface which is modified on its surface with a cross-linked second polymer formed from a polymerizable fluorine containing monomer that contains continuous chain of 5 carbon atoms or less with fluorine atoms, said monomer being polymerized and crosslinked on said membrane, said membrane contains less than 25 ppb of C6 PFCA (Perfluorocarboxylic acid), less than 25 ppb of C8 PFCA and less than 25 ppb of combined C9-14 PFCA.
A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.
A porous electrospun polymeric nanofiber liquid filtration medium, such as an electrospun mats, used for the removal of viral particles (e.g., parvovirus) and other particles in the 18 nm to 30 nm size range from fluid streams, having a mean flow bubble point measured with perfluorohexane above 100 psi. The electrospun medium includes nanofibers having an average fiber diameter of about 6 nm to about 13 nm, and the nanofiber liquid filtration medium has a mean pore size ranging from about 0.01 μm to about 0.03 μm, a porosity ranging from about 80% to about 95%, a thickness ranging from about 1 μm to about 100 μm, and a liquid permeability greater than about 10 LMH/psi. The high porosity of the electrospun mats enable much higher water fluxes, thus reducing the time required to complete virus filtration steps on a fluid stream.
B01D 67/00 - Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
B01D 39/16 - Autres substances filtrantes autoportantes en substance organique, p.ex. fibres synthétiques
A61L 2/02 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contact; Accessoires à cet effet utilisant des phénomènes physiques
41.
Display screen or portion thereof with graphical user interface
An acrylamide gel formulation for use in electrophoresis, and a system and method for electrophoretic gel casting. In some embodiments, the system comprises a UV-Vis curing light source, and an acrylamide formulation that allows a resolving gel and a stacking gel to be photopolymerized in a single step. In some embodiments, the resolving solution formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP) as a water-soluble photoinitiator, which allows the solution to be photopolymerized using UV radiation within a certain wavelength. In some embodiments, a dilution buffer formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP). The dilution buffer may be added to the resolving solution in a selected amount in order to modify the concentration of the acrylamide to a desired concentration.
G01N 27/26 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en utilisant l'électrolyse ou l'électrophorèse
43.
MODULAR INCUBATION CHAMBER AND METHOD OF VIRUS INACTIVATION
An incubation chamber that may be provided in modular form in order to provide flexibility in flow rate and/or residence time of a product stream is disclosed. Assemblies including such incubation chambers for purification of biomolecules are also disclosed, as are methods for biomolecule purification, and in particular, methods for virus inactivation in an incubation chamber or in a plurality of incubation chambers arranged in series.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p.ex. magnétisme, ondes sonores
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
44.
FILTRATION SYSTEM FOR CELL REMOVAL SYSTEMS AND METHODS OF USING THE SAME
A filtration system can include a loading member configured to receive a plurality of filtration members which can be sequentially moved from a non-active position in which a respective one of the plurality of filtration members is not in fluid communication with a sample flow path of the cell removal system, to an active position in which the respective filtration member is in fluid communication with the sample flow path, and to a discarded position in which the respective filtration member has been removed from fluid communication with the sample flow path.
The present application relates to an unsupported, permanently hydrophilic filtration membrane, and its method of formation. The membrane comprises a polymeric matrix material and a cross-linked polyoxazoline hydrophilic additive blended throughout said matrix material.
B01D 67/00 - Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
The present application relates to a multizone, unsupported, microporous, high throughput membrane. The membrane includes a first microporous zone, a second microporous zone, and a third microporous zone, where the third microporous zone is positioned between the first and second microporous zones, with the first, second, and third microporous zones being integral with one another. Further aspects of the present application include a process for making the membrane and a filtration cartridge with the membrane of the present application.
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
B01D 71/82 - Matériaux macromoléculaires non prévus spécifiquement dans un seul des groupes caractérisés par la présence de groupes déterminés, p.ex. introduits par un post-traitement chimique
The disclosure herein relates to a TFF assembly including a baffle comprising a microporous filter within a bioreactor. The baffle may include a collection receptacle to capture a target product or waste materials within the baffle after passing through the microporous filter.
Some embodiments of the present disclosure comprise a flow diverter for use in a biocontainer or bioreactor, comprising a shoulder having a first surface and a second surface opposite the first surface, a conduit extending from the first surface of the shoulder, an inlet at a first end of the conduit, wherein the first end comprises a connector, an outlet formed within the second surface of the shoulder in fluid communication with the inlet at an end opposite the first end of the conduit and a hood adjacent to the outlet, wherein a fluid diverter is capable of directing a fluid down a sidewall of the biocontainer or bioreactor, attenuating a splashing or foaming condition.
Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to filtration to form a clarified product sample; subjecting said clarified product sample to affinity chromatography to remove impurities; subsequently subjecting said product sample to diafiltration followed by virus filtration and optional concentration. The buffer used during the diafiltration step (and thus in the virus filtration step) is the buffer desired for the final formulation of the product.
C07K 1/36 - Extraction; Séparation; Purification par une combinaison de plusieurs procédés de types différents
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12N 7/00 - Virus, p.ex. bactériophages; Compositions les contenant; Leur préparation ou purification
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
C07K 1/34 - Extraction; Séparation; Purification par filtration, ultrafiltration ou osmose inverse
B01D 15/38 - Adsorption sélective, p.ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p.ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
B01D 15/18 - Adsorption sélective, p.ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives aux différents types d'écoulement
B01D 15/36 - Adsorption sélective, p.ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p.ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01D 15/32 - Chromatographie en phase liée, p.ex. avec une phase normale liée, une phase inverse ou une interaction hydrophobe
50.
High capacity composite depth filter media with low extractables
A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.
A probe chamber that includes a holder having a bore capable of holding a sensor or probe; a chamber housing having a central bore in fluid communication with the bore of the holder, wherein the chamber housing can be joined with the holder; and a measurement chamber in fluid communication with the central bore, wherein the measurement chamber comprises a gate, an inlet and an outlet and is capable of receiving a probe tip or sensor tip. Methods for calibrating a probe or sensor are also disclosed.
A multi-layered flexible sparger (100) that has a bottom film layer (102), a middle film layer (106), and a top film layer (110); a first inner mesh (104) disposed between the bottom film layer and the middle film layer; a second inner mesh (114) disposed between the middle film layer and the top film layer; and a port capable of delivering a gas to the multi-layered flexible sparger disposed between the top film layer and the bottom film layer, wherein the middle film layer comprises drill holes and the top film layer comprises drill holes.
A multi-layered flexible sparger (100) that has a bottom film layer (102), a middle film layer (106), and a top film layer (110); a first inner mesh (104) disposed between the bottom film layer and the middle film layer; a second inner mesh (114) disposed between the middle film layer and the top film layer; and a port capable of delivering a gas to the multi-layered flexible sparger disposed between the top film layer and the bottom film layer, wherein the middle film layer comprises drill holes and the top film layer comprises drill holes.
A biocontainer having a first film, the film having an interior and exterior side; articulating elements disposed on or within the first film, the articulating elements comprising at least one a folded hinge, a sealed joint, a thinned pathway, a bowed path, an embedded polymeric or metallic cylindrical fiber or rod; and a second film, optionally comprising articulating elements, joined to the first film, to form a biocontainer having a closed volume, wherein the articulating elements permit the biocontainer to expand and collapse along the articulating elements.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p.ex. par des compteurs de colonies
B32B 27/12 - Produits stratifiés composés essentiellement de résine synthétique adjacente à une couche fibreuse ou filamenteuse
B32B 27/32 - Produits stratifiés composés essentiellement de résine synthétique comprenant des polyoléfines
B32B 3/30 - Produits stratifiés caractérisés essentiellement par le fait qu'une des couches comporte des discontinuités ou des rugosités externes ou internes, ou bien qu'une des couches est de forme générale non plane; Produits stratifiés caractérisés essentiellement par des particularismes de forme caractérisés par une couche comportant des cavités ou des vides internes caractérisés par une couche comportant des retraits ou des saillies, p.ex. des gorges, des nervures
B32B 5/02 - Produits stratifiés caractérisés par l'hétérogénéité ou la structure physique d'une des couches caractérisés par les caractéristiques de structure d'une couche comprenant des fibres ou des filaments
G01M 3/32 - Examen de l'étanchéité des structures ou ouvrages vis-à-vis d'un fluide par utilisation d'un fluide ou en faisant le vide par mesure du taux de perte ou de gain d'un fluide, p.ex. avec des dispositifs réagissant à la pression, avec des indicateurs de débit pour récipients, p.ex. radiateurs
56.
METHODS AND DEVICES FOR ADDING REAGENTS TO BIOREACTORS
A method and device for processing biological fluids that includes providing biological fluids within a cell culture media to a bioreactor and growing biological cells therein; delivering the biological fluid wherein a biological fluid is delivered to the bioreactor via an external re-circulating loop or, for example, a port in a manifold; optionally sampling the biological fluid through sample ports; optionally sensing a condition of the biological fluid; optionally delivering the biological fluid to a second manifold which comprises a weldable sample port; adding a reagent to the biological fluid via the sample port; and returning the biological fluid to the bioreactor.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p.ex. magnétisme, ondes sonores
57.
Display screen or portion thereof with graphical user interface
A holding device (1) for automated stopcock actuation, comprising a base body (2); and a driver (3) rotatably received at the base body (2). The holding device (1) has a receptacle (4) for removably receiving at least a part of a stopcock (A) and is configured such that the driver (3) can engage with and actuate a handle (B) of the stopcock (A) received in the receptacle (4) upon rotation of the driver (3), and a first fixation member (5) that is configured to allow selective fixation of the stopcock (A) in the receptacle (4).
A sampling system can include a cassette assembly coupled to a station base that has a plurality of actuators. The cassette assembly can include a cassette base, a cassette top, and an elastomer membrane disposed between the cassette base and cassette top. The cassette base can include a sample inlet, a reservoir for receiving a sample from the sample inlet, a sample outlet, and a fluid flow path extending between the sample inlet, the reservoir, and the sample outlet.
The disclosure herein relates to a method of reducing background noise during integrity testing of membrane filter to increase sensitivity and more effectively disposition failing membrane filters.
The present invention provides compact spiral-wound filter elements having cassette-like performance. The invention further provides filtration systems (e.g., TFF systems) and processes (e.g., SPTFF processes) employing compact spiral-wound filter elements having cassette-like performance.
Filter elements for perfusion systems and methods are provided. A filter element sheet includes a microporous membrane having a mean pore size of at least about 0.65 μm and a feed spacer comprising woven fibers and having an open area of at least about 35%. The filter element sheet can be arranged within a filter element, for example, in a spiral-wound format or in a cassette format. A perfusion system includes at least one filter element and a pump configured to control flow of a liquid feed through the at least one filter element.
Synthetic, self-replicating RNA vectors comprising sequence encoding at least one immortalization protein, and use of the self-replicating RNA vectors to extend the lifespan of primary cell populations and expand said populations of primary cells.
Synthetic, noninfectious, self-replicating RNA vectors that encode CRISPR proteins are provided. Each self-replicating RNA vector comprises a sequence encoding a plurality of non-structural replication complex proteins from an alphavirus and a sequence encoding a CRISPR protein. Also provided are methods for genome editing in which a synthetic self-replicating RNA vector is transfected into cells along with at least one corresponding guide RNA.
An apparatus for use with an isolator, including a crown for releasably joining with a beta port of the isolator, wherein the crown includes at least one anchoring support; a spring hook on at least one end of the at least one anchoring support; a needle block including at least one through hole for having a needle positioned therein, wherein the at least one needle is joined with tubing; a yoke; and a biasing element releasably joined with the yoke and the at least one anchoring support; wherein the needle block is releasably joined to the crown.
B25J 21/00 - Enceintes à dispositifs de manipulation intégrés
B25J 21/02 - Boîtes à gants, c. à d. enceintes dans lesquelles les manipulations sont exécutées par des mains humaines dans des gants faisant partie de la paroi de l'enceinte; Gants de boîtes à gants
A valve having a body having a first section and a second section; an extended flange attached to the second section of the body or disposed as an integral part of the second section of the body; an elongate bore extending through the body and having a proximal end and a distal end; a longitudinally displaceable plunger disposed in and extending along the bore, the plunger having a proximal end and a distal end and having a first position displaced toward the distal end of the bore and a second position displaced toward the proximal end of the bore; a diaphragm seal attached to the proximal end of the plunger and sealing the bore at the proximal end thereof; a gland seal sealing the bore at a location intermediate the diaphragm seal and the distal end of the bore; the plunger extending through and being sealingly secured to the gland seal; a fluid transfer opening in the bore between the diaphragm seal and the gland seal; longitudinal displacement of the plunger moving the diaphragm seal to open the bore, the gland seal stretching to accommodate the displacement of and maintain a seal about the plunger, a fluid flow path being established between the open proximal end of the bore and the fluid transfer opening, wherein longitudinal displacement of the plunger towards its first position moves the diaphragm to open the bore. The valve further comprises an extended flange having a surface that is approximately coplanar with a surface of the second position when the plunger is displaced toward the proximal end of the bore, creating a zero dead leg position.
F16K 3/26 - Robinets-vannes ou tiroirs, c. à d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation en forme de surfaces de solides de révolution avec corps de tiroir cylindrique le passage du fluide se faisant par le corps du tiroir
F16K 27/04 - Structures des logements; Matériaux utilisés à cet effet des tiroirs
70.
Processes for filtering liquids using single pass tangential flow filtration systems and tangential flow filtration systems with recirculation of retentate
Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p.ex. filtres à aspiration ou à pression, non couverts par les groupes ; Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
B01D 29/52 - Filtres à éléments filtrants stationnaires pendant la filtration, p.ex. filtres à aspiration ou à pression, non couverts par les groupes ; Leurs éléments filtrants à plusieurs éléments filtrants caractérisés par leur agencement relatif montés en parallèle
B01D 29/60 - Filtres à éléments filtrants stationnaires pendant la filtration, p.ex. filtres à aspiration ou à pression, non couverts par les groupes ; Leurs éléments filtrants combinés dans une même structure à des dispositifs de commande de la filtration
The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and flow cytometry detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.
C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p.ex. acides nucléiques
C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
72.
FINAL FILL ASSEMBLY AND METHOD OF INTEGRITY TESTING
Apparatus and methods for redundant filtration assemblies containing filters comprising a multi-purpose vent port are disclosed, wherein the redundant filtration assemblies reduce the amount of components and overall size of the assemblies, promoting the minimization of product losses. A method(s) to conduct pre-use post- sterilization integrity test (PUPSIT) are also disclosed.
A61L 2/00 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contact; Accessoires à cet effet
An apparatus for treating a biological fluid, comprising a plurality of filtration devices. In some embodiments, each of the plurality of filtration devices comprising at least one inlet and at least one outlet; and a first insert plate and a second insert plate opposite the first insert plate, wherein the plurality of filtration devices is disposed therebetween. In some embodiments, each filtration device has a vent port. A manifold for each of the inlets, outlets, and vent ports is optionally fluidly connected. In another embodiment, hose barb connectors or the like may be protected from damage and/or exposure by being recessed in the device or positioned at a periphery of the device. Embodiments include apparatus and methods to achieve dripless connect/disconnect and to reduce the number of sterile-to-sterile connections.
B01D 25/00 - Filtres formés par l'assemblage de plusieurs éléments filtrants fixés ensemble ou parties de tels éléments
B01D 29/00 - Filtres à éléments filtrants stationnaires pendant la filtration, p.ex. filtres à aspiration ou à pression, non couverts par les groupes ; Leurs éléments filtrants
Apparatus and methods for redundant filtration assemblies containing filters comprising a multi-purpose vent port are disclosed, wherein the redundant filtration assemblies reduce the amount of components and overall size of the assemblies, promoting the minimization of product losses. A method(s) to conduct pre-use post- sterilization integrity test (PUPSIT) are also disclosed.
A61L 2/00 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contact; Accessoires à cet effet
A system, software application and method that allows a customer to protect their proprietary database of compounds and substances while utilizing a retrosynthesis software application is disclosed. The customer's proprietary database is encrypted prior to being provided to the retrosynthesis system. This encrypted is performed using a hash and optionally a salt. The retrosynthesis algorithm then creates synthons as is traditionally done. However, after their creation, the synthons are hashed so that they may be compared to the entries in the customer's proprietary database. In this way, the actual contents of the customer's database are never made available to the retrosynthesis system or software application.
G16C 60/00 - Science informatique des matériaux, c. à d. TIC spécialement adaptées à la recherche des propriétés physiques ou chimiques de matériaux ou de phénomènes associés à leur conception, synthèse, traitement, caractérisation ou utilisation
G06F 21/62 - Protection de l’accès à des données via une plate-forme, p.ex. par clés ou règles de contrôle de l’accès
G16C 20/90 - Langages de programmation; Architectures informatiques; Systèmes de bases de données; Stockage de données
76.
CASCADE TANGENTIAL FLOW FILTRATION SYSTEMS FOR PERFUSION OF CELL CULTURE
A multi-stage TFF apparatus, comprising a bioreactor capable of holding cell culture media; a first stage TFF device in fluid communication with the bioreactor; a storage tank in fluid communication with the first stage TFF device; a first recirculating loop in fluid communication with the first stage TFF device and the bioreactor; a second stage TFF device, in fluid communication with and downstream from the storage tank; and a second recirculating loop in fluid communication with the second stage TFF device and the storage tank.
Methods of treating cells are disclosed. The methods include introducing a media comprising at least about 1×106 cells/mL into a perfusion chamber having a volume of 50 mL or less, introducing a volume effective to treat the cells of at least one additive selected from cell culture media, a transducing agent, a pH control agent, and a cell activator into the perfusion chamber, and withdrawing cell waste and byproducts from the perfusion chamber, and harvesting the treated cells. The methods may include introducing the media comprising at least about 3×106 cells/mL into the perfusion chamber. The methods may include measuring and/or controlling at least one parameter of the cells or the media selected from pH, optical density, dissolved oxygen concentration, temperature, and light scattering.
A spiral wound membrane module, comprising a perforated core having an axially extending internal bore; at least one membrane packet comprising a folded membrane sheet defining a first outer face, a first inner face, a second outer face and a second inner face, the fold of the folded membrane sheet being a leading end of the membrane packet; a feed sheet positioned between the first and second inner faces so as to be sandwiched by the folded membrane sheet; a first permeate screen adjacent the first outer face of the membrane sheet defining a first permeate channel; a second permeate screen adjacent the second outer face of the membrane sheet defining a second permeate channel; and a fluid impermeable support coupled to the leading edge of the membrane packet. Also disclosed is a method of manufacturing the spiral wound membrane module.
B01D 65/00 - Accessoires ou opérations auxiliaires, en général, pour les procédés ou les appareils de séparation utilisant des membranes semi-perméables
B01D 63/10 - Modules à membranes enroulées en spirale
The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.
C07K 14/31 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Micrococcaceae (F) provenant de Staphylococcus (G)
B01D 15/38 - Adsorption sélective, p.ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p.ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
B01J 20/286 - Phases reliées chimiquement à un substrat, p.ex. à de la silice ou à des polymères
Depth filters with optimized head space are provided, as well as methods of optimizing the head space in depth filters, and methods of filtration with depth filters having optimized head space, including clarification of flocculated feed streams where pretreatment may include any of lowering cell culture pH, addition of polymers (uncharged or charged), or addition of salts to precipitate out solubilized impurities resulting in high insoluble biomass.
B01D 24/10 - Filtres à substance filtrante non agglomérée, c. à d. à substance filtrante sans aucun liant entre les particules ou les fibres individuelles qui la composent avec le lit filtrant stationnaire pendant la filtration la substance filtrante étant retenue dans un récipient fermé
B01D 39/16 - Autres substances filtrantes autoportantes en substance organique, p.ex. fibres synthétiques
B01D 39/18 - Autres substances filtrantes autoportantes en substance organique, p.ex. fibres synthétiques la substance étant la cellulose ou ses dérivés
B01D 39/20 - Autres substances filtrantes autoportantes en substance inorganique, p.ex. papier d'amiante ou substance filtrante métallique faite de fils métalliques non-tissés
A system for processing a biological fluid that includes a bioreactor, wherein the bioreactor includes a window, at least one port for allowing delivery of a processing aid; a control system, a sensor; a transmitter for transmitting the signal; a signal converter; a controller for receiving the signal; and a valve for delivering the processing aid to the port, wherein the sensor senses a process condition, transmits the signal, and the signal is compared with a reference signal and wherein a process action is optionally taken based on the comparison.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p.ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p.ex. par des compteurs de colonies
83.
IN-LINE PRODUCT CONCENTRATION TO REDUCE VOLUMETRIC LOAD FLOW RATE AND INCREASE PRODUCTIVITY OF A BIND AND ELUTE CHROMATOGRAPHY PURIFICATION
Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to single pass tangential flow filtration to form a concentrated product sample; and subjecting said concentrated product sample to affinity chromatography to remove impurities from said concentrated product sample.
A material for biocontainers comprising a film formed of two or more layers forming a body to the film, the film having an interior and exterior side, and a substrate incorporated in the body of the film wherein the substrate is formed of a fibrous material, wherein each of the at least one of the two or more layers comprises an ethylene vinyl acetate resin. A non-constrained pressure test is also disclosed.
B32B 27/12 - Produits stratifiés composés essentiellement de résine synthétique adjacente à une couche fibreuse ou filamenteuse
B32B 27/30 - Produits stratifiés composés essentiellement de résine synthétique comprenant une résine acrylique
B32B 5/02 - Produits stratifiés caractérisés par l'hétérogénéité ou la structure physique d'une des couches caractérisés par les caractéristiques de structure d'une couche comprenant des fibres ou des filaments
B32B 27/32 - Produits stratifiés composés essentiellement de résine synthétique comprenant des polyoléfines
B32B 27/08 - Produits stratifiés composés essentiellement de résine synthétique comme seul composant ou composant principal d'une couche adjacente à une autre couche d'une substance spécifique d'une résine synthétique d'une sorte différente
B32B 3/26 - Produits stratifiés caractérisés essentiellement par le fait qu'une des couches comporte des discontinuités ou des rugosités externes ou internes, ou bien qu'une des couches est de forme générale non plane; Produits stratifiés caractérisés essentiellement par des particularismes de forme caractérisés par une couche comportant des cavités ou des vides internes
G01M 3/32 - Examen de l'étanchéité des structures ou ouvrages vis-à-vis d'un fluide par utilisation d'un fluide ou en faisant le vide par mesure du taux de perte ou de gain d'un fluide, p.ex. avec des dispositifs réagissant à la pression, avec des indicateurs de débit pour récipients, p.ex. radiateurs
B65D 83/38 - Réceptacles ou paquets comportant des moyens particuliers pour distribuer leur contenu pour distribution de liquides ou de semi-liquides par pression interne de gaz, c. à d. réceptacles aérosols contenant un propulseur - Détails du corps du récipient
A needle protector, having a flexible material, a pocket defining an internal volume, a wrapping strap having a plurality of holes, and a main body disposed between the pocket and the wrapping strap; at least one rigid body or cylinder for placement within the internal volume; and; a pin for placement within one of the plurality of holes, wherein the pin is capable of releasably locking the pocket and the main body when the wrapping strap is wrapped around the pocket and the main body.
B65D 85/24 - Réceptacles, éléments d'emballage ou paquets spécialement adaptés à des objets ou à des matériaux particuliers pour objets incompressibles ou rigides en forme de baguette ou tubulaires pour aiguilles, clous ou petits objets de forme allongée similaires
B65D 33/16 - Dispositifs de fermeture de l'extrémité ou de l'ouverture
A61M 5/32 - Seringues - Parties constitutives - Parties constitutives des aiguilles relatives au raccordement de celles-ci à la seringue ou au manchon; Accessoires pour introduire l'aiguille dans le corps ou l'y maintenir; Dispositifs pour la protection des aiguilles
A filtration module is provided, the module including at least one filtration packet containing filtration media or one or more membranes, such as a stack of membranes, the at least one filtration packet having one or more fluid ports, the one or more fluid ports being surrounded by a primary seal and a secondary seal spaced from the primary seal. The secondary seals are designed to maintain sterility of the assembly during shipping, handling and/or installation. A removable film may cover one or more fluid ports to maintain sterility prior to use.
A funnel-less filtration device that attaches directly to a storage container such as a cell culture media bottle. The device includes a filter collar containing one or more membranes, an inlet with a coupling device for attaching the filter device to a supply of liquid to be filtered, an outlet at a lower portion of the collar, a vacuum port in the collar below the membrane(s) and a filtered vent in the collar above the membrane(s). Optionally, the device may include a filtrate reservoir attached to the outlet, preferably by a threaded connection. Optionally, the upper opening to the filter collar is selectively and removably sealed with a lid until used. The lid is then removed the filter device inverted over a storage container such as cell culture media bottle and the two are attached via the upper opening of the filter collar. The assembly is then inverted so the supply container is above the filter device. Upon subjecting the sample in the bottle to a driving force such as vacuum, the sample flows through the filtration element, and into a filtrate reservoir below the outlet of the filter collar.
Port protection device particularly suited for an encapsulated filter unit. In certain embodiments, the protection device comprises a main body defining an internal region configured to receive a port or the like protruding from an encapsulated filter. In certain embodiments, the main body includes a slot that allows for easy removal of the protection device from the port or the like. A tube management system may be provided to receive and hold tubing. One suitable tube management system includes two spaced tangs that extend from the main body of the protection device and are configured to cooperatively receive and hold tubing between them.
A filtration system can include a loading member configured to receive a plurality of filtration members which can be sequentially moved from a non-active position in which a respective one of the plurality of filtration members is not in fluid communication with a sample flow path of the cell removal system, to an active position in which the respective filtration member is in fluid communication with the sample flow path, and to a discarded position in which the respective filtration member has been removed from fluid communication with the sample flow path.
A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p.ex. filtres à aspiration ou à pression, non couverts par les groupes ; Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
B01D 29/52 - Filtres à éléments filtrants stationnaires pendant la filtration, p.ex. filtres à aspiration ou à pression, non couverts par les groupes ; Leurs éléments filtrants à plusieurs éléments filtrants caractérisés par leur agencement relatif montés en parallèle
B01D 29/60 - Filtres à éléments filtrants stationnaires pendant la filtration, p.ex. filtres à aspiration ou à pression, non couverts par les groupes ; Leurs éléments filtrants combinés dans une même structure à des dispositifs de commande de la filtration
91.
STERILIZABLE POROUS FILTRATION MEDIA CONTAINING NANOFIBER
B01D 67/00 - Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
The invention concerns an installation for treating biological liquid by viral inactivation, comprising a main supply valve (4) for suppling biological liquid to treat, a first line (2) downstream of said valve and provided with a first main tank (7), a second line (3) downstream of said valve and provided with a second main tank (14), said second line being in parallel with said first line, and a third main tank (11) disposed at an outlet both from said first line and said second line and configured to be successively supplied by said first main tank and by said second main tank; said installation being configured such that in each of said first, second and third main treatment tanks, a determined volume of acid and a determined volume of base are introduced at least so as to adjust a pH of said biological liquid and to regulate a rate of drainage flow of said third main treatment tank so as to provide a continuous rate of flow of treated biological liquid at an outlet from said installation.
B01D 39/18 - Autres substances filtrantes autoportantes en substance organique, p.ex. fibres synthétiques la substance étant la cellulose ou ses dérivés
B01D 37/02 - Revêtement préalable des éléments filtrants ou de la substance filtrante; Addition d'adjuvants de filtration au liquide à filtrer
D21C 9/00 - Post-traitement de la pâte de cellulose, p.ex. de la pâte de bois, ou des linters de coton
Some embodiments of the present disclosure comprise a flow diverter for use in a biocontainer or bioreactor, comprising a shoulder having a first surface and a second surface opposite the first surface, a conduit extending from the first surface of the shoulder, an inlet at a first end of the conduit, wherein the first end comprises a connector, an outlet formed within the second surface of the shoulder in fluid communication with the inlet at an end opposite the first end of the conduit and a hood adjacent to the outlet, wherein a fluid diverter is capable of directing a fluid down a sidewall of the biocontainer or bioreactor, attenuating a splashing or foaming condition.
The disclosure herein relates to a TFF assembly including a baffle comprising a microporous filter within a bioreactor. The baffle may include a collection receptacle to capture a target product or waste materials within the baffle after passing through the microporous filter.
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
By keeping track of lists of specific bonds that are to be preserved, a computer program is able to design synthetic routes to create a target compound that avoid that previously published or patented approaches. This may allow the exploration of lower cost or more efficient methods of creating known compounds, or may allow the synthesis of new compunds without the use of patented compunds. Examples of computer-designed syntheses relevant to medicinal chemistry are provided in which the machine avoids “strategic” disconnections common to industrial patents and/or is forced to use different starting materials.
Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to filtration to form a clarified product sample; subjecting said clarified product sample to affinity chromatography to remove impurities; subsequently subjecting said product sample to diafiltration followed by virus filtration and optional concentration. The buffer used during the diafiltration step (and thus in the virus filtration step) is the buffer desired for the final formulation of the product.
Processes and systems for filtering a liquid sample are provided. Batches of a liquid sample can be routed to two or more cycling tanks (e.g., first and second cycling tanks). Upon filling a first cycling tank, a first batch of the liquid sample can be routed to a filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the first cycling tank or to a collection vessel. Upon filling a second cycling tank, a second batch of the liquid sample is routed to the filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the second cycling tank or to the collection vessel. The filling and continuous diafiltration of batches of the liquid sample continues to alternate between the two or more cycling tanks until a total product volume is processed.
The present application relates to an unsupported, permanently hydrophilic filtration membrane, and its method of formation. The membrane comprises a polymeric matrix material and a cross-linked polyoxazoline hydrophilic additive blended throughout said matrix material.
B01D 67/00 - Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
B01D 71/58 - Autres polymères contenant, dans la chaîne principale, uniquement de l'azote, avec ou sans oxygène ou carbone
brevets
