Abrégé

Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.

Classes IPC  ?

• C12Q 1/6823 - Libération de marqueurs de liaison
• C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
• C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
• C12Q 1/6858 - Amplification spécifique d’allèles

Abrégé

Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

Classes IPC  ?

• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Produits et services

Chemical and diagnostic reagents for use as controls in assaying nucleic acids containing cell-free DNA, for scientific or research use; diagnostic reagents for scientific or research use; diagnostic preparations for scientific or research use; assays for research purposes; chemical reagents for scientific or research use, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits thereof, for use in nucleic acid analysis.

Produits et services

Chemical and diagnostic reagents for use as controls in assaying nucleic acids containing cell-free DNA, for scientific or research use; diagnostic reagents for scientific or research use; diagnostic preparations for scientific or research use; assays for research purposes; chemical reagents for scientific or research use, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits thereof, for use in nucleic acid analysis

Produits et services

Chemical reagents for non-medical purposes, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits consisting of oligo nucleotides, buffers, DNA polymerases, enzymes, chemical reagents, modified and non-modified nucleic acids, for use in nucleic acid analysis. Scientific and technological research and design services for others in the field of nucleic acid assays.

Produits et services

(1) Chemical reagents for non-medical purposes, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits consisting of oligo nucleotides, buffers, DNA polymerases, enzymes, chemical reagents, modified and non-modified nucleic acids, for use in nucleic acid analysis (1) Scientific and technological research and design services for others in the field of nucleic acid assays

Abrégé

Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6872 - Méthodes de séquençage faisant intervenir la spectrométrie de masse
• C12Q 1/6844 - Réactions d’amplification d’acides nucléiques
• C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
• C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p.ex. séquençage par hybridation [SBH]
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Produits et services

Chemical reagents for non-medical purposes, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits consisting of oligo nucleotides, buffers, DNA polymerases, enzymes, chemical reagents, modified and non-modified nucleic acids, for use in nucleic acid analysis Scientific and technological research and design services for others in the field of nucleic acid assays

Abrégé

Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

Provided herein are products and processes for the quantitation of mitochondrial nucleic acid in a sample from a subject. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and genomic polynucleotides from nucleic acid for a sample under amplification conditions. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and amplifying sets of nuclear polynucleotides from nucleic acid for a sample under amplification conditions.

Classes IPC  ?

• C12Q 1/6851 - Amplification quantitative
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification

Abrégé

Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.

Classes IPC  ?

• C12Q 1/6823 - Libération de marqueurs de liaison
• C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées

Abrégé

The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

Classes IPC  ?

• C12Q 1/6872 - Méthodes de séquençage faisant intervenir la spectrométrie de masse
• G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

Provided herein are products and processes for primer extension reactions using chain terminating reagents and enzymes having 3' to 5' exonuclease activity, whereby unextended oligonucleotides are digested and extended oligonucleotides are not digested. Also provided herein are products and processes for the detection of the presence, absence or amount of one or more variants of a target nucleic acid species or a plurality of target nucleic acid species comprising primer extension reactions using chain terminating reagents and enzymes having 3' to 5' exonuclease activity, whereby unextended oligonucleotides are digested and extended oligonucleotides are not digested.

Classes IPC  ?

• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme

Abrégé

Provided herein are products and processes for primer extension reactions using chain terminating reagents and enzymes having 3′ to 5′ exonuclease activity, whereby unextended oligonucleotides are digested and extended oligonucleotides are not digested. Also provided herein are products and processes for the detection of the presence, absence or amount of one or more variants of a target nucleic acid species or a plurality of target nucleic acid species comprising primer extension reactions using chain terminating reagents and enzymes having 3′ to 5′ exonuclease activity, whereby unextended oligonucleotides are digested and extended oligonucleotides are not digested.

Classes IPC  ?

• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
• C12Q 1/6813 - Tests d’hybridation

Produits et services

(1) Chemical and diagnostic reagents for use as controls in assaying nucleic acids containing cell-free DNA, for scientific or research use; diagnostic reagents for scientific or research use; diagnostic preparations for scientific or research use; assays for research purposes, namely, laboratory assay kits; chemical reagents for scientific or research use, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits thereof, for use in nucleic acid analysis.

Abrégé

Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Produits et services

Diagnostic reagents for scientific or research use; chemical reagents for scientific or research use, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits thereof, for use in nucleic acid analysis.

Produits et services

Computer software for obtaining and analyzing data relating to nucleic acid design, processing, manipulation, and analysis; computer software for use in a system for distinguishing genetic variations in biological molecules, such as nucleic acid molecules.

Produits et services

(1) Diagnostic reagents for medical-scientific research use; Chemical reagents for scientific or research use, namely, enzymes, buffers, modified and non-modified nucleic acid bases and kits consisting of DNA polymerase, buffer, and nucleotides thereof, for use in nucleic acid analysis

Produits et services

(1) Computer software for obtaining and analyzing data relating to nucleic acid design, processing, manipulation, and analysis; computer software for use in a mass spectrometer-based system for distinguishing genetic variations in biological molecules, such as nucleic acid molecules

Produits et services

Diagnostic reagents for scientific or research use; diagnostic preparations for scientific or research use; assays for research purposes; diagnostic kits consisting primarily of diagnostic reagents for performing pharmacogenetic assays on nucleic acids for scientific or research use.

Produits et services

(1) Diagnostic reagents for medical-scientific research use; diagnostic preparations for medical-scientific research use; biological assays and kits for use in fields of biological and diagnostic testing for industrial, scientific and research use; diagnostic kits consisting primarily of diagnostic reagents for performing pharmacogenetic assays on nucleic acids for scientific or research use

Produits et services

Diagnostic reagents for scientific or research use; Chemical reagents for scientific or research purposes, namely, enzymes, buffers, modified and non-modified nucleic acid bases for laboratory use, and kits consisting of DNA polymerase, buffer, and nucleotides, for use in nucleic acid analysis

Abrégé

Provided herein are multiplex methods for detecting the presence or absence and amount of variants of a plurality of target nucleic acid species having low-abundance variants and high-abundance variants.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme

Produits et services

computer software for obtaining and analyzing data relating to nucleic acid design, processing, manipulation, and analysis; computer software for use in a system for distinguishing genetic variations in biological molecules, such as nucleic acid molecules

Abrégé

Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6872 - Méthodes de séquençage faisant intervenir la spectrométrie de masse
• C12Q 1/6844 - Réactions d’amplification d’acides nucléiques
• C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
• C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p.ex. séquençage par hybridation [SBH]
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Produits et services

diagnostic reagents for scientific or research use; diagnostic preparations for scientific or research use; assays for research purposes; diagnostic kits consisting primarily of diagnostic reagents for performing pharmacogenetic assays on nucleic acids for scientific or research use

Abrégé

The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

Classes IPC  ?

• C12Q 1/6872 - Méthodes de séquençage faisant intervenir la spectrométrie de masse
• G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

Provided herein are products and processes for the quantitation of mitochondrial nucleic acid in a sample from a subject. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and genomic polynucleotides from nucleic acid for a sample under amplification conditions. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and amplifying sets of nuclear polynucleotides from nucleic acid for a sample under amplification conditions.

Classes IPC  ?

• C12Q 1/6851 - Amplification quantitative
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]
• C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
• C12Q 1/6858 - Amplification spécifique d’allèles

Abrégé

Provided herein are products and processes for the quantitation of mitochondrial nucleic acid in a sample from a subject. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and genomic polynucleotides from nucleic acid for a sample under amplification conditions. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and amplifying sets of nuclear polynucleotides from nucleic acid for a sample under amplification conditions.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

Provided herein are compositions and methods useful for preparing and analyzing a sample on a substrate by matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). In some embodiments, compositions provided herein comprise a substrate, matrix and nanoparticles, and sometimes comprise one or more additives and sometimes an analyte. Compositions provided herein sometimes comprise nanoparticles that include or are made up of silicon dioxide.

Classes IPC  ?

• H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p.ex. fermetures étanches au vide; Dispositions pour le réglage externe des composants électronoptiques ou ionoptiques
• H01J 49/16 - Sources d'ions; Canons à ions utilisant une ionisation de surface, p.ex. émission thermo-ionique ou photo-électrique
• G01N 33/483 - Analyse physique de matériau biologique

Abrégé

The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

Classes IPC  ?

• G01N 33/50 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
• G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

Provided herein are multiplex methods for detecting the presence or absence and amount of variants of a plurality of target nucleic acid species having low-abundance variants and high-abundance variants.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6858 - Amplification spécifique d’allèles
• C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Produits et services

System for distinguishing genetic variations in biological molecules, such as nucleic acid molecules, which integrates means for nucleic acid design, processing, manipulation, and analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry comprised primarily of a mass spectrometer, a computer processor, and computer software; computer software for obtaining and analyzing data relating to nucleic acid analysis; scientific measuring apparatus and instruments, namely, silicon chips used in the measurement of biopolymers and nucleic acids.

Produits et services

System for distinguishing genetic variations in biological molecules, such as nucleic acid molecules, which integrates means for nucleic acid design, processing, manipulation, and analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry comprised primarily of a mass spectrometer, a computer processor, and computer software; computer software for obtaining and analyzing data relating to nucleic acid analysis; scientific measuring apparatus and instruments, namely, silicon chips used in the measurement of biopolymers and nucleic acids.

Produits et services

biochemical reagents for scientific and research use; biochemical preparations for scientific and research use; testing kits consisting primarily of biochemical reagents for identifying lung cancer genetic markers for scientific and research use; assays for research purposes

Abrégé

Provided herein are compositions and methods useful for preparing and analyzing a sample on a substrate by matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). In some embodiments, compositions provided herein comprise a substrate, matrix and nanoparticles, and sometimes comprise one or more additives and sometimes an analyte. Compositions provided herein sometimes comprise nanoparticles that include or are made up of silicon dioxide.

Classes IPC  ?

• H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p.ex. fermetures étanches au vide; Dispositions pour le réglage externe des composants électronoptiques ou ionoptiques
• H01J 49/16 - Sources d'ions; Canons à ions utilisant une ionisation de surface, p.ex. émission thermo-ionique ou photo-électrique

Produits et services

System for distinguishing genetic variations in biological molecules, such as nucleic acid molecules, which integrates means for nucleic acid design, processing, manipulation, and analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry comprised primarily of a mass spectrometer, a computer processor, and computer software; computer software for obtaining and analyzing data relating to nucleic acid analysis; scientific measuring apparatus and instruments, namely, silicon chips used in the measurement of biopolymers and nucleic acids

Produits et services

Diagnostic reagents for scientific, research, clinical or medical laboratory use; diagnostic preparations for scientific, research, clinical or medical laboratory use; diagnostic kits consisting primarily of diagnostic reagents for determining blood type genetic markers for scientific, research, clinical or medical laboratory use; assays for research purposes. Scientific research in the field of determining blood type genetic markers; laboratory research in the field of determining blood type genetic markers; clinical research in the field of blood type genetic marker science and medicine; medical research in the field of determining blood type genetic markers; technological research in the field of determining blood type genetic markers; laboratory and clinical scientific analysis in the field of determining blood type genetic markers; genetic testing for scientific research purposes; genetic testing services in the field of determining blood type genetic markers for scientific research purposes.

Produits et services

(1) Diagnostic reagents for scientific, research, clinical or medical laboratory use; diagnostic preparations for scientific, research, clinical or medical laboratory use for testing blood type genetic markers; diagnostic kits consisting primarily of diagnostic reagents for determining blood type genetic markers for scientific, research, clinical or medical laboratory use; assays for research purposes. (1) Scientific research in the field of determining blood type genetic markers; laboratory research in the field of determining blood type genetic markers; clinical research in the field of blood type genetic marker science and medicine; medical research in the field of determining blood type genetic markers; technological research in the field of determining blood type genetic markers; laboratory and clinical scientific analysis in the field of determining blood type genetic markers; genetic testing for scientific research purposes; genetic testing services in the field of determining blood type genetic markers for scientific research purposes.

Produits et services

diagnostic reagents for scientific or research use; diagnostic preparations for scientific or research use; diagnostic kits consisting primarily of diagnostic reagents for determining blood type genetic markers for scientific or research use; assays for research purposes [ scientific research in the field of determining blood type genetic markers; laboratory research in the field of determining blood type genetic markers; genetic testing for scientific research purposes; genetic testing services in the field of determining blood type genetic markers for scientific research purposes ]

Abrégé

Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6823 - Libération de marqueurs de liaison
• C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
• C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
• C12Q 1/6858 - Amplification spécifique d’allèles

Abrégé

Embodiments include integrated robotic sample transfer devices and components thereof which are used for reliably and accurately transferring small samples of material from one registered position to another registered position. Such transfers of material may be carried out by a single pin tool or an array of pin tools of a pin tool head assembly of robotic sample transfer devices. Some embodiments also include automated cleaning of the pin tools used to transfer the sample material. Some embodiments are fully integrated units having internal fluid supply and waste tanks, vacuum source, fluid pumps, controllers and user interface devices.

Classes IPC  ?

• B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en général; Appareils appropriés
• G01N 1/28 - Préparation d'échantillons pour l'analyse
• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
• B01L 3/02 - Burettes; Pipettes
• G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection

Abrégé

The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

Classes IPC  ?

• G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
• G01N 24/00 - Recherche ou analyse des matériaux par l'utilisation de la résonance magnétique nucléaire, de la résonance paramagnétique électronique ou d'autres effets de spin
• G01N 1/00 - Echantillonnage; Préparation des éprouvettes pour la recherche
• C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p.ex. acides nucléiques avec le désoxyribosyle comme radical saccharide

Produits et services

Computer software for obtaining and analyzing data relating to nucleic acid base modifications [ Scientific and technological research and assay design services for others in the field of nucleic acid base-modification analysis ]

Abrégé

Methods, combinations and kits are provided for identifying the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or for identifying the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule. Methylation state identification is performed by treating a methylated target nucleic acid molecule with a reagent that modifies one or more nucleotides in the target nucleic acid molecule as a function of the methylation state of the target nucleic acid molecule, methylation specifically amplifying treated target nucleic acid molecule, fragmenting amplified products, and detecting one or more fragments to thereby identify the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques

Produits et services

(1) Computer hardware and software, and a mass spectrometer for distinguishing genetic variations in biological molecules, such as nucleic acid molecules, which integrates means for nucleic acid design, processing, manipulation, and analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Produits et services

Scientific apparatus and instruments; parts and fittings for the aforementioned goods.

Produits et services

System for distinguishing genetic variations in biological molecules, such as nucleic acid molecules, which integrates means for nucleic acid design, processing, manipulation, and analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry comprised primarily of a mass spectrometer, a computer processor, and computer software

Produits et services

Providing analysis and diagnostic services in the field of science; diagnosis and analysis of biopolymers.

Produits et services

Scientific measuring apparatus and instruments, namely, silicon chips used in the measurement of biopolymers and nucleic acids [, proteins, peptides, carbohydrates, and lipids ]