Abrégé

The present invention relates to a method of detecting a target microparticle in a fluid in a microfluidic particle analysis device comprising a measuring channel with a first electrode and a second electrode defining an operating space between and being in electrical connection with an electric current source and a device for monitoring an electrical signal from the first and/or the second electrode. A sample fluid suspected of containing a target microparticle exposing an identification binding partner is mixed with a recognition binding partner component to provide a complex of the recognition binding partner and the identification binding partner, and the complex is labelled with electrically conducting nanoparticles; before optionally adjusting the conductivity to be in the range of 5,000 μS/cm to 50,000 μS/cm and applying a flow of the suspension to the measuring channel of the microfluidic particle analysis device; before applying a current to create an electric field in the operating space and monitoring an electrical signal between the first and the second electrode to detect target microparticles labelled with the electrically conducting nanoparticles. The method is suited to detect a pathogenic bacterium in a water or another fluid.

Classes IPC  ?

• G01N 15/1031 - Recherche de particules individuelles en mesurant des effets électriques ou magnétiques
• G01N 15/10 - Recherche de particules individuelles

Abrégé

The present invention relates to a microfluidic particle analysis device comprising an inlet and an outlet in fluid communication via a main channel defining a main flow direction from an inlet end to an outlet end, the main channel being defined by a main channel wall extending from the inlet end to the outlet end and having a first cross-sectional dimension in the range of 20 μm to 120 μm and a second cross-sectional dimension of at least 100 μm, the main channel wall at a vertex having an opening extending along the main flow direction and being open to an analysis section having surfaces at an analytical distance in the range of 5 μm to 50 μm and a sensor system for detecting a particle. The present invention also relates to a method of using the microfluidic particle analysis device for detecting particles or monitoring the concentration of particles.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• G01N 15/1031 - Recherche de particules individuelles en mesurant des effets électriques ou magnétiques
• G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux

Abrégé

The present invention relates to a method of detecting a target microparticle in a fluid in a microfluidic particle analysis device comprising a measuring channel with a first electrode and a second electrode defining an operating space between and being in electrical connection with an electric current source and a device for monitoring an electrical signal from the first and/or the second electrode. A sample fluid suspected of containing a target microparticle exposing an identification binding partner is mixed with a recognition binding partner component to provide a complex of the recognition binding partner and the identification binding partner, and the complex is labelled with electrically conducting nanoparticles; before optionally adjusting the conductivity to be in the range of 5,000 µS/cm to 50,000 µS/cm and applying a flow of the suspension to the measuring channel of the microfluidic particle analysis device; before applying a current to create an electric field in the operating space and monitoring an electrical signal between the first and the second electrode to detect target microparticles labelled with the electrically conducting nanoparticles. The method is suited to detect a pathogenic bacterium in a water or another fluid.

Classes IPC  ?

• G01N 15/10 - Recherche de particules individuelles
• G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide

Abrégé

The present invention relates to a microfluidic particle analysis device comprising an inlet and an outlet in fluid communication via a main channel defining a main flow direction from an inlet end to an outlet end, the main channel being defined by a main channel wall extending from the inlet end to the outlet end and having a first cross-sectional dimension in the range of 20 μm to 120 μm and a second cross-sectional dimension of at least 100 μm, the main channel wall at a vertex having an opening extending along the main flow direction and being open to an analysis section having surfaces at an analytical distance in the range of 5 μm to 50 μm and a sensor system for detecting a particle. The present invention also relates to a method of using the microfluidic particle analysis device for detecting particles or monitoring the concentration of particles.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• G01N 15/10 - Recherche de particules individuelles
• G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux

Produits et services

Measuring apparatus and instruments; analytical apparatus and instruments; laboratory and scientific apparatus and instruments; parts and fittings for the aforesaid goods, including sampling devices for obtaining test samples; devices for providing an indication of cellular or microbial contamination; measuring apparatus for measuring bacteria; digital microbiological apparatus; measuring apparatus for measuring bacteria based on flow cytometry; apparatus for analysing bacteria concentrations in aqueous solutions; hygiene monitoring systems; microbial monitoring systems; food hygiene indicators; analysis instruments for use in food research; instruments for measuring microbiology and bacteria in relation to ensuring hygiene and product quality in food businesses, hospitals, pharmaceutical manufacturers, and companies supplying drinking water and process water; instruments for measuring bacteria in bathing water, including sea water, spas and swimming pools; instruments for measuring microbiology and bacteria in liquid and product samples; apparatus and instruments for measuring surfaces; test kits and instrumentation for the determination of biological, chemical and physical constituents in fluid and solid samples; software, facilitating the collection of analysis data from industrial processes and subsequent analysis of the aforesaid data.

Produits et services

Measuring apparatus and instruments for quantifying bacteria concentrations in a sample; analytical apparatus and instruments for quantifying bacteria concentrations in a sample; laboratory and scientific apparatus and instruments, namely, measuring apparatus for quantifying bacteria concentrations in a sample; component parts and fittings for the aforesaid goods; sampling devices for obtaining test samples, namely, swabs and vials for laboratory use; devices for detecting cellular and microbial contamination; measuring apparatus for measuring the quantity of bacteria; digital microbiological quantifying apparatus; measuring apparatus for measuring the quantity of bacteria based on flow cytometry; apparatus for analysing bacteria concentrations in aqueous solutions; hygiene monitoring systems comprised of a measuring apparatus for quantifying bacteria concentrations in a sample; microbial monitoring systems comprised of a measuring apparatus for quantifying bacteria concentrations in a sample; food hygiene indicators, namely, apparatus for analysing bacteria concentrations in food; analysis instruments for use in food research, namely, apparatus for analysing bacteria concentrations in food; instruments for measuring the quantity of microbes and bacteria in relation to ensuring hygiene and product quality in food businesses, hospitals, pharmaceutical manufacturers, and companies supplying drinking water and process water; instruments for measuring the quantity of bacteria in bathing water, sea water, spas, and swimming pools; instruments for measuring the quantity of microbes and bacteria in liquid and product samples; apparatus and instruments for measuring the quantity of bacteria on surfaces; test kits and instrumentation, namely, devices for detecting cellular and microbial contamination in biological, chemical, and physical constituents in fluid and solid samples; downloadable software for facilitating the collection of analysis data from industrial processes and subsequent analysis of the aforesaid data

Abrégé

A microfluidic particle analysis device comprising an inlet with an inlet manifold providing parallel fluid communication with a bypass channel and a measuring channel having a sensor system for detecting a particle, wherein the angle of the measuring channel relative to the main flow direction is in the range of 0° to 60°, and wherein the angle of the bypass channel relative to the main flow direction is in the range of 0° to 60°. The present invention also relates to a method of using the device microfluidic particle analysis.

Classes IPC  ?

• G01R 27/08 - Mesure de la résistance par mesure à la fois de la tension et de l'intensité
• G01N 15/10 - Recherche de particules individuelles
• G01N 1/20 - Dispositifs pour prélever des échantillons à l'état liquide ou fluide pour matériau coulant ou s'éboulant
• G01N 15/06 - Recherche de la concentration des suspensions de particules