Abrégé

The present invention provides a signal calibration method for imaging mass cytometry, comprising: performing forward linear regression and reverse linear regression on a sample signal by means of a standard substance signal: first, selecting one sample as a standard sample, and establishing a first standard curve and a first regression model according to a standard substance on a slide of the sample; then, establishing a second standard curve and a second regression model according to a standard substance on a slide of a sample to be calibrated; scanning the sample to be calibrated in a first resolution, performing log processing on an obtained original signal value, and inputting the original signal value into the second regression model to obtain an actual metal content of each pixel in the sample to be calibrated; and finally, converting the actual metal content of each pixel into a calibration signal value by means of the first regression model, completing data calibration, and further eliminating an influence caused by instrument sensitivity fluctuations.

Classes IPC  ?

• G01N 27/626 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'ionisation des gaz, p. ex. des aérosolsRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant les décharges électriques, p. ex. l'émission cathodique utilisant la chaleur pour ioniser un gaz

Abrégé

Provided in the present invention is a signal calibration method for imaging mass cytometry, which method performs forward and reverse linear regressions on a sample signal by means of a standard substance signal. The method comprises: first selecting a sample as a standard sample, and establishing a first standard curve and a first regression model according to a standard substance on a slide thereof; then establishing a second standard curve and a second regression model according to a standard substance on a slide of a sample to be subjected to calibration; then scanning, by using a first resolution, the sample to be subjected to calibration, performing log processing on an obtained original signal value, and inputting, into the second regression model, the original signal value that has been subjected to the log processing, so as to obtain the actual metal content of each pixel of the sample to be subjected to calibration; and finally, converting the actual metal content of each pixel into a calibrated signal value by means of the first regression model, so as to complete data calibration, thereby eliminating the influence caused by sensitivity fluctuations of an instrument.

Classes IPC  ?

• G01N 27/626 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'ionisation des gaz, p. ex. des aérosolsRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant les décharges électriques, p. ex. l'émission cathodique utilisant la chaleur pour ioniser un gaz

Abrégé

The present disclosure provides a model and method for rapid screening the efficacy of immunoregulatory drugs. The method comprises constructing an animal model containing PVDF capsule tubes, and further comprises analyzing the pharmacodynamic data for the animal model in vivo, phenotype identification by paired flow cytometry and multi-omics data. The PVDF capsule tubes have cells comprising tumor cells and immune cells in a single cell suspension; wherein the tumor cells and immune cells are derived from fresh tumor tissues or body fluids of clinical patients. The animal model constructed according to the present disclosure can be used for immune system targeting and drug efficacy regulation screening, and drug screening for multiple patients at the same time. It provides a rapid and effective method and model for clinical precision medicine and new drug development.

Classes IPC  ?

• C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
• A01K 67/027 - Nouvelles races ou races modifiées de vertébrés

Abrégé

The disclosure provides methods for rapid screening of the efficacy of immunoregulatory drugs, especially for cancer drug screening and treatment. The methods comprise an animal model wherein polymeric capsule tubes contain cells comprising tumor cells and immune cells in a single cell suspension. The capsule device is implanted into a mouse that is treated with an anti-cancer drug, especially an immunomodulatory anti-cancer drug. The methods provide a rapid and effective way for determining a specific individual's clinical response to an immunomodulatory drug with extremely high speed and subsequent efficacious treatment in the clinical setting.

Classes IPC  ?

• C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
• A61K 45/06 - Mélanges d'ingrédients actifs sans caractérisation chimique, p. ex. composés antiphlogistiques et pour le cœur
• A61P 35/00 - Agents anticancéreux
• C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK

Abrégé

The present invention provides a method for determining the efficacy of anti-tumor drugs in a time-efficient manner, comprising the following steps: (1) transferring a primary tumor cell into an implant device; (2) implanting the implant device comprising the primary tumor cell into an animal; (3) administering a candidate drug to the animal; (4) determining the sensitivity of the tumor cell to the candidate drug.

Classes IPC  ?

• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
• A61K 49/00 - Préparations pour examen in vivo
• A01K 67/027 - Nouvelles races ou races modifiées de vertébrés
• A61K 9/00 - Préparations médicinales caractérisées par un aspect particulier

Produits et services

Technological research; research and development of new products for others; scientific laboratory services; scientific research and development; pharmaceutical research services; pharmaceutical product evaluation; genetic screening [for scientific purposes]; bacteriological research; biological research; clinical trials.

Produits et services

Technological research in the field of pharmaceuticals; research and development of new products for others; scientific laboratory services; scientific research and development; pharmaceutical research services; pharmaceutical product evaluation; genetic testing for scientific research purposes; bacteriological research; biological research; scientific research in the nature of conducting clinical trials for others

Produits et services

Chemical research; chemical analysis; chemistry services; bacteriological research; biological research; clinical trials; testing of pharmaceuticals; technological research; research and development of new products for others; scientific laboratory services.

Abrégé

Provided are a human non-small cell lung cancer (NSCLC) cell line, a reagent and a kit comprising the present human NSCLC cell lines. Also provided are the use of evaluating the efficacy of anti-NSCLC drugs by using the human NSCLC cell lines and the method for producing the animal model in testing efficacy of drugs or obtaining pharmacological data for said drugs.

Classes IPC  ?

• C12N 5/09 - Cellules tumorales
• C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
• A61K 49/00 - Préparations pour examen in vivo
• C12R 1/91 - Lignées cellulaires

Produits et services

Chemical research; chemical analysis; chemistry consultation; bacteriological research; biological research; scientific research in the nature of conducting clinical trials for others; testing of pharmaceuticals; technological research in the field of pharmaceuticals; research and development of new products for others; scientific laboratory services

Abrégé

A method for obtaining an animal model from conditionally reprogrammed tumor cells for screening anti-tumor drugs and a method for screening anti-tumor drugs using the same.

Classes IPC  ?

• C12N 5/09 - Cellules tumorales
• A61K 49/00 - Préparations pour examen in vivo
• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique

Abrégé

A method for obtaining an animal model from conditionally reprogrammed tumor cells for screening anti-tumor drugs and a method for screening anti-tumor drugs using the same.

Classes IPC  ?

• C12N 5/09 - Cellules tumorales
• C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
• A61K 49/00 - Préparations pour examen in vivo
• C12R 1/91 - Lignées cellulaires

Abrégé

A method for obtaining an animal model from conditionally reprogrammed tumor cells for screening anti-tumor drugs and a method for screening anti-tumor drugs using the same.

Classes IPC  ?

• C12N 5/09 - Cellules tumorales
• A61K 49/00 - Préparations pour examen in vivo
• C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables

Abrégé

A rapid method for testing the drug efficacy of anti-tumor drugs, comprising the following steps: (1) placing primary tumor cells into an implant device; (2) implanting the implant device containing the primary tumor cells into the body of an animal subject; (3) administrating a drug to be tested to the animal subject; and (4) detecting the sensitivity of tumor cells to the drug to be tested.

Classes IPC  ?

• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
• G01N 33/15 - Préparations médicinales
• A61K 49/00 - Préparations pour examen in vivo