A method of preparing a circular RNA includes transcribing a vector to form a precursor RNA, in which the vector includes the following elements operably connected to each other and arranged in the following sequence: a) a 5′ element, b) a 3′ Group I self-splicing intron fragment containing a 3′ splice site dinucleotide, c) none or an element containing an internal ribosome entry site (IRES) and a protein coding region or an element containing a noncoding region, d) a 5′ Group I self-splicing intron fragment containing a 5′ splice site dinucleotide, and e) a 3′ element, in which 5′ element and 3′ element form a stable structure with a Gibbs free energy (ΔG) from −190 kcal/mol to −9.0 kcal/mol, provided that the stable structure is not a duplex with at least 95% base pairing between 5′ element and 3′ element, in which 3′ Group I self-splicing intron fragment and 5′ Group I self-splicing intron fragment form a self-cleaving and self-ligating RNA molecule, thereby generating circular RNA.
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
C12N 15/66 - Méthodes générales pour insérer un gène dans un vecteur pour former un vecteur recombinant, utilisant le clivage et la ligatureUtilisation de linkers non fonctionnels ou d'adaptateurs, p. ex. linkers contenant la séquence pour une endonucléase de restriction
C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
2.
METHODS AND COMPOSITIONS FOR PRIMER-INDEPENDENT CELL-FREE DNA AMPLIFICATION
A method for amplifying a DNA template, comprising: a) providing a composition comprising a DNA template, an RNA polymerase, a DNA polymerase, and a deoxyribonucleotide, wherein said composition does not comprise a ribonucleotide; and b) incubating said composition in a suitable condition to allow amplification of said DNA template. Also provided is a method for priming DNA amplification from a DNA template, comprising: incubating a DNA template, an RNA polymerase and a deoxyribonucleotide in a suitable condition to allow the initiation of DNA amplification; wherein said method does not comprise adding a ribonucleotide.
C12Q 1/25 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des enzymes qui ne peuvent pas être classées dans les groupes
C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
C12Q 1/533 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une isomérase
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
3.
METHODS OF PREPARING CAPPED MRNA WITH SITE-SPECIFIC MODIFICATIONS
A method for producing a site-specific modified capped recombinant RNA includes (a) obtaining a first RNA that is monophosphorylated at the 5' end and contains the site-specific modification, (b) reacting an activated capping compound of Formula (I), Formular (II), or Formula (III) with the 5' monophosphorylated first RNA obtained from (a) in the presence of a heteroaromatic compound, a metal salt, and a solvent, hereby producing a 5' capped first RNA, (c) obtaining a second RNA that is monophosphorylated at the 5' end, and (d) ligating the 3' end of the 5' capped first RNA obtained from (b) to the 5' end of the second RNA obtained from (c), thereby producing the site-specific modified capped recombinant RNA.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
4.
COMPOSITIONS AND METHODS FOR PREPARING CAPPED MRNA
A method for in vitro transcription of a DNA template into RNA includes providing a mixture containing a buffer substance, ribonucleoside triphosphates (NTPs), one or more magnesium salts in a concentration of from about 2 mM to about 60 mM, the DNA template, and a recombinant RNA polymerase, and incubating the reaction mixture at from about 25° C. to about 40° C. for from about 1 hour to about 12 hours thereby producing the RNA. A method for in vitro transcription includes providing a DNA template and a cap analogue that binds to −1 and/or +1 nucleotides of promoter for in vitro transcription, thus producing more full length mRNAs, allowing for more flexibility on the choice of first mRNA base, and providing+2 position open for custom sequence.
A method of preparing a circular RNA includes transcribing a vector to form a precursor RNA, in which the vector includes the following elements operably connected to each other and arranged in the following sequence: a) a 5′ element, b) a 3′ Group I self-splicing intron fragment containing a 3′ splice site dinucleotide, c) none or an element containing an internal ribosome entry site (IRES) and a protein coding region or an element containing a noncoding region, d) a 5′ Group I self-splicing intron fragment containing a 5′ splice site dinucleotide, and e) a 3′ element, in which 5′ element and 3′ element form a stable structure with a Gibbs free energy (ΔG) from −190 kcal/mol to −9.0 kcal/mol, provided that the stable structure is not a duplex with at least 95% base pairing between the 5′ element and 3′ element, in which 3′ Group I self-splicing intron fragment and 5′ Group I self-splicing intron fragment form a self-cleaving and self-ligating RNA molecule, thereby generating circular RNA.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
C12N 15/66 - Méthodes générales pour insérer un gène dans un vecteur pour former un vecteur recombinant, utilisant le clivage et la ligatureUtilisation de linkers non fonctionnels ou d'adaptateurs, p. ex. linkers contenant la séquence pour une endonucléase de restriction
C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
Functionalized aryldiazonium salts and films formed by electrografting of functionalized aryldiazonium salts are provided. Methods for purifying functionalized aryldiazonium salts and for coating solid support systems with functionalized aryldiazonium salts are also provided. These coated solid support systems can be used, for example, in methods of oligonucleotide synthesis.
C25B 11/095 - Électrodes comportant des électro-catalyseurs sur un substrat ou un support caractérisées par le matériau électro-catalytique formé d’au moins un élément catalytique et d’au moins un composé catalytiqueÉlectrodes comportant des électro-catalyseurs sur un substrat ou un support caractérisées par le matériau électro-catalytique formé de plusieurs éléments catalytiques ou composés catalytiques au moins un des composés est de type organique
C25D 9/02 - Revêtement électrolytique autrement qu'avec des métaux avec des matières organiques
C25F 1/00 - Nettoyage, dégraissage, décapage ou enlèvement de battitures par voie électrolytique
7.
IN VITRO TRANSCRIPTION METHODS AND COMPOUNDS FOR USE THEREIN
in vitroin vitro transcription of a DNA template into RNA includes providing a mixture containing a buffer substance, ribonucleoside triphosphates (NTPs), one or more magnesium salts in a concentration of from about 2 mM to about 60 mM, the DNA template, a recombinant RNA polymerase, and a cap analogue comprising the structure of Formula (I), (II), or (III), and incubating the reaction mixture at from about 15°C to about 35°C, optionally from about 18°C to about 31°C, for from about 1 hour to about 12 hours, thereby producing the RNA.
Provided herein are methods and compositions for oligonucleotide synthesis utilizing universal linker phosphoramidites. Methods and reagents are described with DNA synthesis using controlled pore glass (CPG) solid supports, and on platinum coated electrodes for electrochemical DNA synthesis. The universal linkers can be used as spacers in single-column PCR primer synthesis to generate 2 strands with free 3′-hydroxy termini after cleavage. The methods and compositions utilize a solid support system for synthesis of oligonucleotides, wherein the support has platinum electrodes and a universal linker, optionally wherein the platinum electrode is coated with an amine. The methods and compositions further describe use of universal linker phosphoramidites and the platinum electrode is coated with a monosaccharide, or a disaccharide.
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C07C 247/04 - Composés contenant des groupes azido avec des groupes azido liés à des atomes de carbone acycliques d'un squelette carboné étant saturé
C07C 275/10 - Dérivés d'urée, c.-à-d. composés contenant l'un des groupes les atomes d'azote ne faisant pas partie de groupes nitro ou nitroso ayant des atomes d'azote de groupes urée liés à des atomes de carbone acycliques d'un squelette carboné acyclique et saturé étant substitué de plus par des atomes d'oxygène liés par des liaisons simples
A microarray chip, a system comprising the microarray chip, and methods for testing or using the microarray chip are provided. The microarray chip includes an electrode array comprising a plurality of cells. Each of the plurality of cells comprises an anode and a cathode, each of which comprise an electrically conductive material. The microarray chip further includes a plurality of current comparator circuits. Each current comparator circuit includes a first current mirror connected with a reference current source, a first capacitor connected with the first current mirror, a second current mirror configured to be connected with one of the plurality of cells, and a second capacitor and a third capacitor connected in parallel and connected with the second current mirror. Each current comparator circuit also includes two voltage comparators configured to compare the voltages from the capacitors and provide digital signals selected from 0 and 1.
A method of preparing a circular RNA includes transcribing a vector to form a precursor RNA, in which the vector includes the following elements operably connected to each other and arranged in the following sequence: a) a 5' element, b) a 3' Group I self-splicing intron fragment containing a 3' splice site dinucleotide, c) none or an element containing an internal ribosome entry site (IRES) and a protein coding region or an element containing a noncoding region, d) a 5' Group I selfsplicing intron fragment containing a 5' splice site dinucleotide, and e) a 3' element, in which the 5' element and the 3' element form a stable structure with a Gibbs free energy (ΔG) from -190 kcal/mol to -9.0 kcal/mol, provided that the stable structure is not a duplex with at least 95% base pairing between the 5' element and the 3' element, in which the 3' Group I self-splicing intron fragment and the 5' Group I selfsplicing intron fragment form a self-cleaving and self-ligating RNA molecule, thereby generating circular RNA.
A01N 3/00 - Conservation de végétaux ou de parties de ceux-ci, p. ex. par inhibition de l'évaporation, avec amélioration de l'aspect des feuillesMastic à greffer
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion
12.
COMPOSITIONS AND METHODS FOR PREPARING CAPPED MRNA
A01N 3/00 - Conservation de végétaux ou de parties de ceux-ci, p. ex. par inhibition de l'évaporation, avec amélioration de l'aspect des feuillesMastic à greffer
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion
13.
Compositions and methods for preparing capped mRNA
A method for in vitro transcription of a DNA template into RNA includes providing a mixture containing a buffer substance, ribonucleoside triphosphates (NTPs), one or more magnesium salts in a concentration of from about 2 mM to about 60 mM, the DNA template, and a recombinant RNA polymerase, and incubating the reaction mixture at from about 25° C. to about 40° C. for from about 1 hour to about 12 hours thereby producing the RNA. A method for in vitro transcription includes providing a DNA template and a cap analogue that binds to −1 and/or +1 nucleotides of promoter for in vitro transcription, thus producing more full length mRNAs, allowing for more flexibility on the choice of first mRNA base, and providing +2 position open for custom sequence.
A61K 31/7115 - Acides nucléiques ou oligonucléotides ayant des bases modifiées, c.-à-d. autres que l'adénine, la guanine, la cytosine, l'uracile ou la thymine
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
C12N 9/64 - Protéinases provenant de tissu animal, p. ex. rennine
14.
AUTOMATED INTEGRATED SYSTEM FOR CONTINUOUS COMMERCIAL-SCALE PLASMID PRODUCTION
Functionalized aryldiazonium salts and films formed by electrografting of functionalized aryldiazonium salts are provided. Methods for purifying functionalized aryldiazonium salts and for coating solid support systems with functionalized aryldiazonium salts are also provided. These coated solid support systems can be used, for example, in methods of oligonucleotide synthesis.
Provided herein are methods and compositions for oligonucleotide synthesis utilizing universal linker phosphoramidites. Methods and reagents are described with DNA synthesis using controlled pore glass (CPG) solid supports, and on platinum coated electrodes for electrochemical DNA synthesis. The universal linkers can be used as spacers in single-column PCR primer synthesis to generate 2 strands with free 3'-hydroxy termini after cleavage. The methods and compositions utilize a solid support system for synthesis of oligonucleotides, wherein the support has platinum electrodes and a universal linker, optionally wherein the platinum electrode is coated with an amine. The methods and compositions further describe use of universal linker phosphoramidites and the platinum electrode is coated with a monosaccharide, or a disaccharide.
C07D 487/02 - Composés hétérocycliques contenant des atomes d'azote comme uniques hétéro-atomes dans le système condensé, non prévus par les groupes dans lesquels le système condensé contient deux hétérocycles
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
B82B 3/00 - Fabrication ou traitement des nanostructures par manipulation d’atomes ou de molécules, ou d’ensembles limités d’atomes ou de molécules un à un comme des unités individuelles
17.
AUTOMATED INTEGRATED SYSTEM FOR CONTINUOUS COMMERCIAL-SCALE PLASMID PRODUCTION
09 - Appareils et instruments scientifiques et électriques
Produits et services
Laboratory apparatus for use in protein purification; laboratory apparatus and computer systems comprised of electric power, control system, operating circuit and shell for use in protein purification [ ; scientific apparatus and instruments for measuring relative DNA/RNA and protein and parts and fittings therefor; electrically powered scientific apparatus for use in protein analysis, detection, separation and purification ]
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Biological research; Chemical, biochemical, biological and bacteriological research and analysis; Computer software design; Conducting clinical trials for others; Pharmaceutical drug development services; Pharmaceutical research and development; Product quality testing services; Research and development of new products for others; Research in the field of chemistry; Technical research in the field of pharmaceutical studies; Providing a web hosting platform for researchers to exchange biomaterials and ideas to promote innovation in the field of biology
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Biological research; Chemical, biochemical, biological and bacteriological research and analysis; Computer software design; Conducting clinical trials for others; Pharmaceutical drug development services; Pharmaceutical research and development; Product quality testing services; Research and development of new products for others; Research in the field of chemistry; Technical research in the field of pharmaceutical studies; Providing a web hosting platform for researchers to exchange biomaterials and ideas to promote innovation in the field of biology
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Technical research; Quality testing; Biochemical research and analysis; Chemical analysis; Genetic testing for scientific research purposes; Biological cloning services; Biological research and analysis; Clinical trials; Material testing; Computer system design.
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Technical research in the field of pharmaceutical studies; bacteriological research; biological research; Chemistry consultation; chemical research; Consulting services for others in the field of design, planning, and implementation project management of scientific research; research and development of new products for others; chemical analysis; cosmetic research; scientific laboratory services
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Scientific, technological and medical research services, namely, the service of providing cloning, protein expression, peptide synthesis and antibody production
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
39 - Services de transport, emballage et entreposage; organisation de voyages
Produits et services
Preservation in the nature of refrigeration or cryogenic preservation of biological materials; Hazardous waste management of biological materials Storage of biological materials
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
39 - Services de transport, emballage et entreposage; organisation de voyages
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
biological materials preservation services storage of biological materials scientific and technological services, namely, scientific research in the field of biology
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
scientific research and technical research services, namely, production of stable cell lines by using lentivirus technology; scientific research and technical research services relating to producing, assaying and purifying recombinant or manipulated lentivirus
