Abrégé

An apparatus for biomolecule analysis, comprising a plurality of cells (1) comprising a well (10) extending along the axis (X) from a top surface (4) towards a bottom surface (2), a molecule (24) anchored to the bottom surface (2) and a bead (22) within the well; an electrically conductive solution filling the well (10); a top electrode (14) and bottom electrode (18) at the bottom surface (2) in contact with the electrically conductive solution, wherein a cavity (20) wider than the well (10) is arranged between the bottom electrode (18) and the well (10), said cavity (20) in fluid communication with the well (10) and exposing the bottom electrode (18) at the bottom surface to the electrically conductive solution filling the cavity (20).

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

A device for optically imaging at least a part of an object, the device having an optical axis and including a two-dimensional first array of first microlenses, having a first side intended to face the object, and a second side, opposite the first side, a two-dimensional second array of second microlenses, each first microlens being aligned with a second microlens on an axis parallel to the optical axis, wherein each first microlens comprises a first catoptric system, and preferably a first catadioptric system.

Classes IPC  ?

• G02B 17/08 - Systèmes catadioptriques
• G02B 5/10 - Miroirs à surfaces courbes
• G02B 17/00 - Systèmes avec surfaces réfléchissantes, avec ou sans éléments de réfraction
• G02B 17/06 - Systèmes catoptriques, p. ex. systèmes redressant et renversant une image utilisant uniquement des miroirs

Abrégé

An apparatus for biomolecule analysis, comprising a plurality of cells (1) comprising a well (10) extending along the axis (X) from a top surface (4) towards a bottom surface (2), a molecule (24) anchored to the bottom surface (2) and a bead (22) within the well; an electrically conductive solution filling the well (10); a top electrode (14) and bottom electrode (18) at the bottom surface (2) in contact with the electrically conductive solution, wherein a cavity (20) wider than the well (10) is arranged between the bottom electrode (18) and the well (10), said cavity (20) in fluid communication with the well (10) and exposing the bottom electrode (18) at the bottom surface to the electrically conductive solution filling the cavity (20).

Classes IPC  ?

• G01N 27/327 - Électrodes biochimiques
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• C12Q 1/6825 - Détecteurs faisant intervenir la détection d’acides nucléiques

Abrégé

The present invention relates to a method of isolating a nucleic acid target region from a population of nucleic acid molecules, said method comprising the steps of a) contacting said population of nucleic acid molecules with a Class 2 Type V Cas protein-gRNA complex, wherein the gRNA comprises a guide segment that is complementary to a first site adjacent to said target region, thereby forming a Class 2 Type V Cas protein-gRNA-nucleic acid complex, b) contacting the population of nucleic acid molecules comprising said Class 2 Type V Cas protein-gRNA-nucleic acid complex with at least one enzyme having single-strand 3′ to 5′ exonuclease activity, thereby forming a 5′ single-stranded overhang in said first site, c) removing the Class 2 Type V Cas protein-gRNA complex from the population of step b), d) contacting the population of step c) with an oligonucleotide probe, said probe comprising a sequence that is at least partially complementary to said overhang, thereby forming a duplex between said probe and said overhang, and e) isolating said duplex from the population of nucleic acid molecules of step d), thereby isolating said nucleic acid target region.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction

Abrégé

The present invention relates to a method for the isolation of a target nucleic acid region. In particular, said method comprises the steps of contacting a population of nucleic acid molecules with at least one Type II Cas protein-gRNA complex, wherein said gRNA comprises a guide segment that is complementary to the sequence comprised in the target region of at least one nucleic acid molecule, thereby forming a Type II Cas protein-gRNA-nucleic acid complex, contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, and isolating the target nucleic acid region from the Type II Cas protein-gRNA-nucleic acid complex.

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12N 9/22 - Ribonucléases

Abrégé

2, the ratio of the second refractive index over the first refractive index being lower than 1, preferably lower than 0.66.

Classes IPC  ?

• G02B 3/00 - Lentilles simples ou composées
• G02B 13/24 - Objectifs optiques spécialement conçus pour les emplois spécifiés ci-dessous pour reproduire ou copier à de courtes distances de l'objet

Abrégé

The present invention relates to a method of isolating a nucleic acid target region from a population of nucleic acid molecules, said method comprising the steps of a) contacting said population of nucleic acid molecules with a Class 2 Type V Cas protein-gRNA complex, wherein the gRNA comprises a guide segment that is complementary to a first site adjacent to said target region, thereby forming a Class 2 Type V Cas protein-gRNA-nucleic acid complex, b) contacting the population of nucleic acid molecules comprising said Class 2 Type V Cas protein-gRNA-nucleic acid complex with at least one enzyme having single-strand 3′ to 5′ exonuclease activity, thereby forming a 5′ single-stranded overhang in said first site, c) removing the Class 2 Type V Cas protein-gRNA complex from the population of step b), d) contacting the population of step c) with an oligonucleotide probe, said probe comprising a sequence that is at least partially complementary to said overhang, thereby forming a duplex between said probe and said overhang, and e) isolating said duplex from the population of nucleic acid molecules of step d), thereby isolating said nucleic acid target region.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction

Abrégé

A device for optically imaging at least a part of an object, the device having an optical axis and including a two-dimensional first array of first microlenses, having a first side intended to face the object, and a second side, opposite the first side, a two-dimensional second array of second microlenses, each first microlens being aligned with a second microlens on an axis parallel to the optical axis, wherein each first microlens comprises a first catoptric system, and preferably a first catadioptric system.

Classes IPC  ?

• G02B 17/08 - Systèmes catadioptriques
• G02B 5/10 - Miroirs à surfaces courbes
• G02B 17/00 - Systèmes avec surfaces réfléchissantes, avec ou sans éléments de réfraction

Abrégé

The present invention relates to a method of preparing a nucleic acid molecule comprising a target region for isolation from a sample, said method comprising the steps of contacting a population of nucleic acid molecules with a first Class 2 Type V Cas protein-gRNA complex, wherein the gRNA comprises a guide segment that is complementary to a first sequence within a nucleic acid molecule, said first sequence being located adjacent to said target region, thereby forming a Class 2 Type V Cas protein-gRNA-nucleic acid complex, and a protector molecule, contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, recovering said nucleic acid molecule comprising the target region from the Class 2 Type V Cas protein-gRNA-nucleic acid complex formed in step a), and contacting the nucleic acid molecule of step c) with a processive polymerase, preferably a mesophilic or thermophilic polymerase, even more preferably a T4 DNA polymerase.

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12N 9/22 - Ribonucléases

Abrégé

2, the ratio of the second refractive index over the first refractive index being lower than 1, preferably lower than 0.66.

Classes IPC  ?

• G02B 3/00 - Lentilles simples ou composées
• G02B 13/24 - Objectifs optiques spécialement conçus pour les emplois spécifiés ci-dessous pour reproduire ou copier à de courtes distances de l'objet

Abrégé

The present invention relates to a device for optically imaging at least a part of an object, the device having an optical axis and comprising a two- dimensional first array of first microlenses, having a first side intended to face the object, and a second side, opposite the first side, a two-dimensional second array of second microlenses, each first microlens being aligned with a second microlens on an axis parallel to the optical axis, wherein each first microlens comprises a first catoptric system, and preferably a first catadioptric system.

Classes IPC  ?

• G02B 17/00 - Systèmes avec surfaces réfléchissantes, avec ou sans éléments de réfraction
• G02B 17/06 - Systèmes catoptriques, p. ex. systèmes redressant et renversant une image utilisant uniquement des miroirs
• G02B 17/08 - Systèmes catadioptriques
• G02B 5/10 - Miroirs à surfaces courbes

Abrégé

The present invention relates to a method of isolating a nucleic acid target region from a population of nucleic acid molecules,said method comprising the steps of a) contacting said population of nucleic acid molecules with a Class 2 Type V Cas protein-gRNA complex, wherein the gRNA comprises a guide segment that is complementary to a first site adjacent to said target region, thereby forming a Class 2 Type V Cas protein-gRNA-nucleic acid complex, b) contacting the population of nucleic acid molecules comprising said Class 2 Type V Cas protein-gRNA-nucleic acid complex with at least one enzyme having single-strand 3' to 5' exonuclease activity, thereby forming a 5' single-stranded overhang in said first site, c) removing the Class 2 Type V Cas protein-gRNA complex from the population of step b), d) contacting the population of step c) with an oligonucleotide probe, said probe comprising a sequence that is at least partially complementary to said overhang, thereby forming a duplex between said probe and said overhang, and e) isolating said duplex from the population of nucleic acid molecules of step d), thereby isolating said nucleicacid target region.

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]

Abrégé

The present invention relates to a method for the isolation of a target nucleic acid region. In particular, said method comprises the steps of contacting a population of nucleic acid molecules with at least one Type II Cas protein-gRNA complex, wherein said gRNA comprises a guide segment that is complementary to the sequence comprised in the target region of at least one nucleic acid molecule, thereby forming a Type II Cas protein-gRNA-nucleic acid complex, contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, and isolating the target nucleic acid region from the Type II Cas protein-gRNA-nucleic acid complex.

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12N 9/22 - Ribonucléases

Abrégé

The present invention relates to a method of preparing a nucleic acid molecule comprising a target region for isolation from a sample, said method comprising the steps of contacting a population of nucleic acid molecules with a first Class 2 Type V Cas protein-gRNA complex, wherein the gRNA comprises a guide segment that is complementary to a first sequence within a nucleic acid molecule, said first sequence being located adjacent to said target region, thereby forming a Class 2 Type V Cas protein-gRNA-nucleic acid complex, and a protector molecule,contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, recovering said nucleic acid molecule comprising the target region from the Class 2 Type V Cas protein-gRNA-nucleic acid complex formed in step a), and contacting the nucleic acid molecule of step c) with a processive polymerase, preferably a mesophilic or thermophilic polymerase, even more preferably a T4 DNA polymerase.

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• C12N 9/22 - Ribonucléases

Abrégé

Ln11 n22 2 , the ratio of the second refractive index over the first refractive index being lower than 1, preferably lower than 0.66.

Classes IPC  ?

• G02B 13/24 - Objectifs optiques spécialement conçus pour les emplois spécifiés ci-dessous pour reproduire ou copier à de courtes distances de l'objet
• G02B 3/00 - Lentilles simples ou composées

Abrégé

The present invention relates to a method for the isolation of a target nucleic acid region. In particular, said method comprises the steps of contacting a population of nucleic acid molecules with at least one Type II Cas protein -gRNA complex, wherein said gRNA comprises a guide segment that is complementary to the sequence comprised in the target region of at least one nucleic acid molecule, thereby forming a Type II Cas protein-gRNA-nucleic acid complex, contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, and isolating the target nucleic acid region from the Type II Cas protein-gRNA-nucleic acid complex.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques