A genetically modified microorganism which satisfies at least one condition selected from the group consisting of the following conditions (I) and (II). Condition (I): citrate synthase activity is reduced or inactivated compared with a wild-type microorganism corresponding to the genetically modified microorganism, and condition (II): oxaloacetate decarboxylase activity is reduced or inactivated compared with the wild-type microorganism.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The present disclosure provides a method and an apparatus with which a target substance can be produced more efficiently from a lignocellulose-based material. The target substance is produced by: (a) allowing saccharification and a microbial reaction to proceed in parallel in a reaction solution containing a pretreated lignocellulose-based material, at least one saccharifying enzyme, and a microorganism, thereby producing at least one target substance; (b) subjecting at least a portion of the reaction solution to a solid-liquid separation treatment during or after the saccharification and the microbial reaction in step (a), thereby obtaining a fraction (X) containing the at least one saccharifying enzyme and a fraction (Y) containing the microorganism and a reaction residue; and (c) circulating at least a portion of the at least one saccharifying enzyme contained in the fraction (X) obtained in step (b) back to the reaction solution in step (a).
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
3.
RECOMBINANT MICROORGANISM AND L-LEUCINE PRODUCTION METHOD USING SAME
The present disclosure relates to a recombinant microorganism capable of efficiently producing L-leucine and an L-leucine production method using the same. The production method includes: (p) incubating a recombinant microorganism having an L-leucine biosynthesis pathway or a processed product of microbial cells thereof in a predetermined culture medium (X) to thereby produce L-leucine; and (q) recovering a fraction including the L-leucine from the predetermined culture medium (X). The recombinant microorganism includes, in an expressible form, a gene encoding an amino acid dehydrogenase. The amino acid dehydrogenase at least has a catalytic effect on an L-leucine production reaction in the L-leucine biosynthesis pathway.
Provided is an aspartic acid composition containing aspartic acid and an impurity, the impurity containing at least one selected from the group consisting of an amino acid other than aspartic acid, an organic acid, and salts of the amino acid other than aspartic acid and the organic acid, a content of the impurity being 0.02% by mass or more and 1.1% by mass or less. Also provided is a method for producing polysuccinimide, the method including performing a polymerization reaction of the aspartic acid using the aspartic acid composition. Also provided is a polysuccinimide composition produced by the method for producing polysuccinimide.
The present disclosure pertains to a method for producing aspartic acid whereby impurities can be reduced or eliminated even when a crude product contaminated with a considerable amount of impurities such as amino acids, organic substances, colorants, and inorganic salts is used as a starting material. The method comprises: (q) preparing a slurry of a crystalline fraction (X) containing β-type crystals of aspartic acid and at least one impurity; and (r) heating the slurry to convert the β-type crystals of aspartic acid to α-type crystals and obtain a crystalline fraction (Y) containing aspartic acid in the α-type crystals.
L-aspartic acid having excellent powder handleability (degree of compaction) and little adhesion of mother liquor to the crystals can be provided according to the present invention. L-aspartic acid containing these L-aspartic acid crystals can also be provided. The L-aspartic acid crystals of the present invention have a shaped isotropic crystal shape.
C07C 229/24 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant plus d'un groupe carboxyle lié au squelette carboné, p. ex. acide aspartique
7.
GENETICALLY MODIFIED MICROORGANISM AND METHOD FOR PRODUCING ASPARAGINE
This genetically modified microorganism satisfies at least one condition selected from the group consisting of conditions (I) and (II). Condition (I): in comparison with a wild-type microorganism corresponding to the genetically modified microorganism, citrate synthase activity is reduced or inactivated. Condition (II): in comparison with the wild-type microorgansm, oxaloacetate decarboxylase activity is reduced or inactivated.
An aspartic acid composition including aspartic acid and an impurity, wherein the impurity includes at least one type of impurity selected from the group consiting of amino acids other than aspartic acid, organic acids, and salts of these, and the impurity content is 0.02 mass% to 1.1 mass%. A polysuccinimide production method that includes using said aspartic acid composition and subjecting said aspartic acid to a polymerization reaction. A polysuccinimide composition that is produced using said polysuccinimide production method.
The present disclosure pertains to a method for producing aspartic acid whereby impurities can be reduced or eliminated even when a crude product contaminated with a considerable amount of impurities such as amino acids, organic substances, colorants, and inorganic salts is used as a starting material. The method comprises: (q) preparing a slurry of a crystalline fraction (X) containing β-type crystals of aspartic acid and at least one impurity; and (r) heating the slurry to convert the β-type crystals of aspartic acid to α-type crystals and obtain a crystalline fraction (Y) containing aspartic acid in the α-type crystals.
C07C 229/24 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant plus d'un groupe carboxyle lié au squelette carboné, p. ex. acide aspartique
The present disclosure relates to a recombinant microorganism capable of efficiently generating L-leucine and an L-leucine production method using the recombinant microorganism. The production method includes (p) generating L-leucine by incubating a recombinant microorganism having an L-leucine biosynthetic pathway or a microbial processed product thereof in a predetermined culture medium (X) and (q) recovering a fraction including the L-leucine from the predetermined culture medium (X). The recombinant microorganism includes, in an expressible form, a gene for encoding an amino acid dehydrogenase. The amino acid dehydrogenase has a catalyst effect on at least the L-leucine generation reaction in the L-leucine biosynthetic pathway.
The present disclosure provides a novel IPMS mutant which can impart, to various microorganisms, the ability to produce a derivative including L-leucine or a precursor thereof. The IPMS mutant contains at least two amino acid substitutions selected from the following (a') to (c') on the basis of the amino acid sequence set forth in SEQ ID NO: 2: (a') substitution of the amino acid residue corresponding to the glycine residue at position 530 with an amino acid residue other than a glycine residue; (b') substitution of the amino acid residue corresponding to the glycine residue at position 532 with an amino acid residue other than a glycine residue; and (c') substitution of the amino acid residue corresponding to the alanine residue at position 535 with an amino acid residue other than an alanine residue.
The present disclosure relates to a genetically modified microorganism satisfying some of predetermined conditions. The predetermined conditions include: (I) succinate dehydrogenase activity or fumarate reductase activity being reduced or inactivated relative to a wild-type microorganism; (II) lactate dehydrogenase activity being reduced or inactivated relative to the wild-type microorganism; (III) the genetically modified microorganism having modified phosphoenolpyruvate carboxylase activity showing resistance to feedback inhibition by aspartic acid in wild-type phosphoenolpyruvate carboxylase activity, or exogenous phosphoenolpyruvate carboxylase activity having higher resistance to feedback inhibition by aspartic acid than that of the wild-type phosphoenolpyruvate carboxylase activity shown by the wild-type microorganism; and (IV) pyruvate:quinone oxidoreductase being reduced or inactivated relative to the wild-type microorganism.
C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
In the field of chemical products, for chemical products of the same composition, it is difficult to recognize differences caused by differences in production method and it is also very difficult to guarantee traceability because individual identification, as for devices and the like, is not possible. This usage history management system uses identifiers that specify raw materials to perform usage history management of the raw materials used in a series of processes and thereby makes it possible to find the quantities of specific raw materials used in a product, even for products, such as products produced by biorefining using biomass as raw materials, that have different production processes but use two or more chemicals of the same composition as raw materials. The usage history management system also uses blockchain to increase the certainty of the authenticity of information for guaranteeing traceability.
Provided is a method of producing a target substance from a starting substance via an NADH-accumulating reaction pathway, the method comprising: incubating bacteria under an aerobic condition; and subsequently incubating the bacteria under an anaerobic condition in the presence of the starting substance and nitrate ion to produce the target substance.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
A method for manufacturing 1,3-propanediol includes culturing, in the presence of a saccharide and formaldehyde to produce 1,3-propanediol, a microorganism having the following genes: (a) a first gene encoding an enzyme that catalyzes an aldol reaction between pyruvic acid and aldehydes; (b) a second gene encoding an enzyme that catalyzes a decarboxylation reaction of α-keto acids; and (c) a third gene encoding an enzyme that catalyzes a reduction reaction of aldehydes, is provided.
RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH (Japon)
GREEN EARTH INSTITUTE CO.,LTD. (Japon)
Inventeur(s)
Inui Masayuki
Hiraga Kazumi
Suda Masako
Kato Naoto
Watanabe Akira
Oide Shinichi
Abrégé
Provided are: a transformant which can produce 1,3-butanediol in a biological manner; and a method for producing 1,3-butanediol using the transformant. In one aspect, a transformant produced by introducing a gene encoding an alcohol dehydrogenase belonging to the cinnamyl-alcohol dehydrogenase (CAD) family into a host bacterium, wherein the host bacterium has a mutant or a gene introduced thereinto in such a manner that at least one of the enzymes (A) to (D) can be expressed or the expression of the at least one of the enzymes can be induced. (A) Acetyl-CoA carboxylase; (B) acetyl-CoA synthetase; (C) acetyl-CoA reductase; and (D) 3-3-hydroxybutyryl-CoA reductase.
The present disclosure pertains to a genetically modified microorganism that satisfies some of specific requirements. The specific requirements include: (I) compared to a wild type microorganism, having reduced or inactivated succinate dehydrogenase activity or fumarate reductase activity; (II) compared to a wild type microorganism, having reduced or inactivated lactate dehydrogenase activity; (III) having a modified phosphoenolpyruvate carboxylase activity and thus showing resistance against the feedback inhibition by aspartic acid in wild type phosphoenolpyruvate carboxylase activity, or having an exogenous phosphoenolpyruvate carboxylase activity and thus showing higher resistance against the feedback inhibition by aspartic acid than wild type phosphoenolpyruvate carboxylase activity shown by a wild type microorganism; and (IV) compared to a wild type microorganism, having reduced or inactivated pyruvate : quinone oxidoreductase.
A method for producing an α-keto acid, said method comprising a step of culturing a microorganism, which contains a gene encoding an enzyme catalyzing an aldol reaction between pyruvic acid and a carbonyl compound, in the presence of a pyruvic acid-supplying compound selected from pyruvic acid and a saccharide and a carbonyl compound selected from an aldehyde and a ketone to thereby produce the α-keto acid.
This method for producing 1,3-propanediol includes culturing microorganisms that include the following genes: (a) a first gene encoding an enzyme that catalyzes an aldol reaction between pyruvic acid and aldehydes, (b) a second gene encoding an enzyme that catalyzes a decarboxylation reaction of α-keto acids, and (c) a third gene encoding an enzyme that catalyzes a reduction reaction of aldehydes, the microorganisms being cultured in the presence of a saccharide and formaldehyde to produce 1,3-propanediol.
Provided is a method of producing a target substance from a starting substance via an NADH-accumulating reaction pathway, the method comprising: incubating bacteria under an aerobic condition; and subsequently incubating the bacteria under an anaerobic condition in the presence of the starting substance and nitrate ion to produce the target substance.
The present disclosure relates to: a nucleic acid that includes a gene expression control sequence that can control the expression of a target gene in coryneform bacteria; bacteria into which the nucleic acid has been introduced; a method for using the bacteria to express the target gene; and a method for using the bacteria to produce a substance. At least a portion of the nucleic acid has an arbitrary promoter sequence and an AraR-binding sequence that is specified by a prescribed nucleotide sequence. When the nucleic acid has been introduced into coryneform bacteria, the promoter activity of the promoter sequence is suppressed so long as arabinose is not added and is induced when arabinose is added.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
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