A freshwater fish antiviral medicament comprising the following ingredients proportioned on the basis of mass fraction: folium isatidis (35 to 45), nepeta (25 to 35), arctium lappa (25 to 35), gentiana scabra (15 to 25), centipeda minima (15 to 25), and lithospermum purpurocaeruleum (15 to 25). The antiviral medicament is provided with enhanced inhibitory effect on a freshwater fish virus and is applicable in large-scale freshwater fish farms.
A livestock intestinal sustained-release anthelmintic comprising a medicament particle (3), an intermediate layer (2), and a surface layer (1). The intermediate layer (2) covers the exterior surface of the medicament particle (3), while the surface layer (1) covers the exterior surface of the intermediate layer (2). The intermediate layer (2) and the surface layer (1) of the sustained-release anthelmintic implement sustained-release of a medicament, thus preventing the medicament from being decomposed and destroyed in stomach, while also preventing the medicament from causing stomach irritation and adverse reactions.
S & E ANIMAL PHARMACEUTICAL (TIANJIN）CO., LTD. (Chine)
Inventeur(s)
Zhang, Yong
Wang, Lihong
Liu, Dingkuo
Abrégé
Provided is a method for preparing and renaturing chicken interferon γ, comprising the following steps: (1) performing PCR amplification on the genes of chicken interferon γ; (2) subcloning the product of step (1) to a pET32a expression vector having a 6-His tag; (3) transforming the product of step (2) into cells of Escherichia coli M15; (4) performing induction and expression on the product of step (3), then cracking to acquire a protein expression product; (5) purifying the expression product of step (4); and (6) renaturing recombinant protein purified to acquire a product. Employment of the pET32a and of Escherichia coli M15 allows for the expression product of chicken interferon γ gene to reach after purification a purity of 95%, which is greater than the 85% purity of a conventional method, and for the renaturation to reach a success rate between 38% and 39%, which is greater than the 20% success rate of the conventional method. Increase of active ingredient in total protein prepared improves treatment effects and reduces costs.
S ＆ E Animal Pharmaceutical （TianJin）Co ., LTD. (Chine)
Inventeur(s)
Dong, Huifeng
Abrégé
A method for preparing and extracting extracellular lactase is provided, which comprises the following steps: (1) inoculating activated Actinomucor elegans strain into a solid culture medium to culture; (2) adding inorganic flocculants into cultured crude enzyme solution to precipitate it, then filtering to obtain a semi-finished product; (3) the obtained semi-finished product is salted out by ammonium sulfate grading sedimentation method and purified by Sephadex G-100 gel chromatography and diethylaminoethyl cellulose (DEAE)-cellulose-52 ion exchange chromatography to obtain the lactase finished product. Compared with the method for producing lactase intracellularly in the art, the method of the present application is simple and suitable for large scale manufacturing of extracellular lactase, and provides extracellular lactase with high heat resistance and good stability. And the working pH of the present method is suitable for the internal biological environment of the organism.
C12N 9/38 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons bêta-galactose-glycoside, p. ex. bêta-galactosidase
S ＆ E ANIMAL PHARMACEUTICAL (TIANJIN) CO., LTD. (Chine)
Inventeur(s)
Dong, Huifeng
Abrégé
An injection of enrofloxacin and the producing method thereof are disclosed. The injection comprises from 10 to 20 parts of enrofloxacin, from 0.1 to 0.5 part of antioxidant and from 0.1 to 0.5 part of chelating agent and has a neutral pH value. The antioxidant is sodium bisulfite or sodium thiosulfate. The chelating agent is EDTA. The injection is stable and not irritative. Its producing method comprises the steps of: (1) adding enrofloxacin to the aqueous solution of sodium hydroxide; (2) continuing adding the aqueous solution of sodium hydroxide to obtain a pH value of 9-13; (3) adding acidifying agent to obtain a neutral pH value; (4) adding the antioxidant; (5) adding the chelating agent; (6) adding water to obtain the final product. The acidifying agent in step (3) is one or two or three selected from the group of lactic acid, sodium butyrate and arginine.
An aqueous formulation of vitamins and the preparation method thereof. Based on the weight, the raw material composition of the aqueous formulation includes: liposomes, 1.89~5.85 parts vitamin A, 2~4 parts vitamin D, 1~3 parts vitamin E, 1~2 parts vitamin K, 4~6 parts vitamin B1, 2~4 parts vitamin B2, 2~6 parts vitamin B6, 1~2 parts vitamin B12, 1~6 parts vitamin C, 2~4 parts vitamin H, 1~2 parts folic acid, and solubilizers.
A61K 9/127 - Vecteurs à bicouches synthétiques, p. ex. liposomes ou liposomes comportant du cholestérol en tant qu’unique agent tensioactif non phosphatidylique
A61K 31/714 - Cobalamines, p. ex. cyanocobalamine, vitamine B12
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