A purification column, comprising a column body (1), an absorption film (3), and a plug (2). The column body (1) is a cylinder body having an open top and a sealed bottom; a liquid outlet (101) is formed at the bottom end of the cylinder body; the liquid outlet (101) is communicated with an inner cavity of the cylinder body; the absorption film (3) is laid on the inner bottom surface of the cylinder body and covers the liquid outlet (101); the plug (2) is provided on the absorption film (3) and used for blocking the bottom end of the cylinder body; the plug (2) comprises a supporting wall (202) and a covering film (201) that are connected together; upper and lower ends of the supporting wall (202) are open; the covering film (201) is connected to an end face of the supporting wall (202) away from the absorption film (3), and covers an opening at this end of the supporting wall (202).
A pre-packaged reagent plate, comprising a reagent plate body (1) and seal films. The reagent plate body (1) is provided with at least one through hole (A). The seal films are used for sealing two ports of each through hole (A). An accommodating cavity is formed in the interior of each through hole (A) between the two seal films and is used for accommodating a reaction reagent. The seal films can be pierced to release the reaction reagent.
Provided are a nucleic acid extraction and purification device and a biochemical molecule extraction and purification device, belonging to the field of experimental supplies. Both devices have the functionality of a test tube, and thus can hold a liquid to perform preprocessing extraction and purification of nucleic acids or biochemical molecules such as nucleic acids, i.e. achieving a process of cell rupture. Furthermore, both devices can quickly and easily transfer liquid to the extraction membrane, achieving the extraction and purification of nucleic acids or biochemical molecules such as nucleic acids. Furthermore, the two devices do not need to manually fit with a pipettor during the process of liquid transfer or use an automated robotic arm and a mechanical pipettor to complete the process of liquid transfer.
B01D 29/00 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
B01D 29/03 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants plats autoportants
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
A nucleic acid extraction system, comprising: a nucleic acid extraction plate, and a pipetting device and positive pressure device that are arranged side by side, the pipetting device comprising: an infusion device, an infusion device mounting frame, an infusion device driving unit, and a plurality of infusion units, each of the infusion units comprising: a reagent supply device and a reagent flow controller; the positive pressure device comprises: a plug, an air pipe, a positive pressure provider and a positive pressure driving device. The nucleic acid extraction system is highly efficiency and low-cost when extracting nucleic acid.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/00 - Dispositifs de supportDispositifs de serrage
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
B01F 27/806 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical les agitateurs ou les récipients étant déplacés afin de les mettre en position de fonctionnementMoyens de fixation du récipient avec déplacement vertical de l'agitateur, p. ex. en combinaison avec des moyens permettant de faire pivoter l'agitateur autour d'un axe vertical afin de coopérer avec différents récipients
A nucleic acid extraction system, comprising: a nucleic acid extraction plate, and a pipetting device and positive pressure device that are arranged side by side, the pipetting device comprising: an infusion device, an infusion device mounting frame, an infusion device driving unit, and a plurality of infusion units, each of the infusion units comprising: a reagent supply device and a reagent flow controller; the positive pressure device comprises: a plug, an air pipe, a positive pressure provider and a positive pressure driving device. The nucleic acid extraction system is highly efficiency and low-cost when extracting nucleic acid.
Provided are a nucleic acid extraction and purification device and a biochemical molecule extraction and purification device, belonging to the field of experimental supplies. Both devices have the functionality of a test tube, and thus can hold a liquid to perform preprocessing extraction and purification of nucleic acids or biochemical molecules such as nucleic acids, i.e. achieving a process of cell rupture. Furthermore, both devices can quickly and easily transfer liquid to the extraction membrane, achieving the extraction and purification of nucleic acids or biochemical molecules such as nucleic acids. Furthermore, the two devices do not need to manually fit with a pipettor during the process of liquid transfer or use an automated robotic arm and a mechanical pipettor to complete the process of liquid transfer; at the same time, the two devices are small and lightweight, such that the extraction and purification of nucleic acids or biochemical molecules such as nucleic acids is highly efficient and low in cost.
A nucleic acid extraction and filtration column, belonging to the field of experimental consumable Items. The nucleic acid extraction and filtration column has a top portion provided with a liquid inlet, the nucleic acid extraction and filtration column has a bottom portion provided with a liquid outlet, and the nucleic acid extraction and filtration column comprises: a plurality of filtering members, the plurality of filtering members being arranged between the liquid inlet and the liquid outlet and being fixed on the inner wall of the nucleic acid extraction and filtration column; each of the filtering members is provided with a plurality of filtering holes; in two adjacent filtering members, the pore size of the filtering holes of the filtering member close to the top portion of the nucleic acid extraction and filtration column is larger than the pore size of the filtering holes of the filtering member close to the bottom portion of the nucleic acid extraction and filtration column. Nucleic acid solution to be extracted sequentially passes through the plurality of filtering members, so that solid impurities are totally blocked by the plurality of filtering members in order of the volume sizes, and nucleic acid can, along with the liquid phase, smoothly and quickly pass through the plurality of filtering members sequentially, thereby improving filtering efficiency during nucleic acid extraction.
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