A bioreactor includes a housing with a number of removable parts for modular construction and easy assembly or disassembly. The removable parts include a fixed bed and a support for the fixed bed. More than one support may be provided. The multiple supports may interlock with each other or a lid of the bioreactor. The support interlocks with the housing to prevent relative rotation. The multiple supports include a support frame for supporting the fixed bed an allowing fluid to flow through the support frame. The fixed bed forms a peripheral chamber between the support and the housing to hold the fixed bed. One or more probes and tubes may be inserted into the bioreactor to test parameters of or to add to or remove liquid from inside.
A system for biomass sensing includes a fixed bed bioreactor including a container and a fixed bed disposed within such container. At least one sensor is for sensing one or more parameters representative of biomass in the fixed bed. A controller is adapted for correlating the one or more parameters to an amount of biomass of the fixed bed bioreactor, such as using a correlation model. Related systems and methods are also described.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
3.
SYSTEM AND METHOD FOR THE PRODUCTION OF BIOMOLECULES SUCH AS VIRAL VACCINES
The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retenate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.
An apparatus is provided for culturing and transfecting cells using a liquid. A bioreactor includes a cell culture bed for receiving the liquid. An external loop is connected to the bioreactor and adapted for circulating the liquid to and from the bioreactor. The external loop is adapted for introducing a gas into the liquid therein and/or for circulating a transfection material in the liquid therein to the bioreactor. In one embodiment, a first vessel is associated with the external loop for receiving the liquid, and a gas injector is provided for injecting a gas into the liquid, such as into the first vessel. A second vessel is also provided for providing a transfection material (such as a transfecting agent as part of a transfection mixture) to the liquid, such as directly to the bioreactor or to the external loop. In another embodiment, the gas injector is associated directly with the external loop. The external loop may be dedicated for gas injection and/or transfection, and maintained separate from a loop for circulating liquid media to the bioreactor, which potentially allows for operation on demand. Related methods are also disclosed.
Systems and methods for harvesting cells include a bioreactor with a structure for cell entrapment/adherence and growth, such as a fixed bed structure. A cell harvest mechanism is adapted to agitate the bioreactor, such as by vibrating or shaking the fixed bed, and to move a liquid level relative to the structure, such as by repeatedly flushing and filling the bioreactor. A cell detaching solution is provided to the bioreactor, such as an enzymatic cocktail for the detachment of cells without producing clumps or aggregates. The bioreactor may comprise a preculture vessel upstream of another bioreactor.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
7.
SYSTEMS AND METHODS FOR THE CONTINUOUS PRODUCTION AND PURIFICATION OF BIOLOGICS
A system for producing and purifying target biologics is disclosed. A cell culture unit is provided for generating target biologics in a volume of fluid. A purification unit is also provided for purifying the target biologics in the volume of fluid portion directly from the cell culture unit without a clarifying step, such as by using one or more separators, such as expanded beds or chromatography columns arranged in parallel, and potentially operating in series to achieve continuous operation. The one or more separators may include magnetic beads adapted for binding to the target biologics. The purification unit may receive at least a portion of the volume of fluid directly from the cell culture unit, and a collection unit may be provided for collecting the purified target biologics from the purification unit, and also may be adapted for returning the volume of fluid without the target biologics to the cell culture unit. The system may comprise a first vessel adapted to concentrate a volume of fluid including the target biologics, and a second vessel adapted to further concentrating the volume of fluid, the first or second vessels including magnetic beads adapted for binding with the target biologics. Related methods are also disclosed.
A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.
The current invention relates to a method for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor, said method comprises: the addition of a lysis solution to the bioreactor, thereby lysing the cells in the bioreactor, and prior to, simultaneous with, and/or after lysing said cells, applying one or more mechanical actions to said bioreactor, after which the one or more target biomolecules are recovered from said bioreactor. The invention further relates to a system for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor.
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
The current invention relates to a method for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor, said method comprises: - the addition of a lysis solution to the bioreactor, thereby lysing the cells in the bioreactor, and prior to, simultaneous with, and/or after lysing said cells, applying one or more mechanical actions to said bioreactor, after which the one or more target biomolecules are recovered from said bioreactor. The invention further relates to a system for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor.
The current disclosure relates to a biomolecule production system for producing biomolecules, wherein said system comprises one or more vessels, such as a bioreactor vessel, a transfer vessel, a reagent vessel, a waste vessel and/or a collection vessel, and optionally a concentrator, wherein one or more of said vessels are equipped with at least a first and a second pressure sensor, said first pressure sensor measuring the hydrostatic pressure in the vessel and said second pressure sensor measuring the gas headspace pressure (such as air pressure) in said vessel.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
12.
STRUCTURED MONOLITHIC FIXED BED FOR CELL CULTURE, RELATED BIOREACTOR AND METHODS OF MANUFACTURING
An apparatus for culturing cells includes a bioreactor including a monolithic structured cell culture bed, which may be fabricated using additive manufacturing techniques, such as for example 3D printing. The bioreactor and monolithic structured cell culture bed may be formed as a unitary structure. The monolithic structured cell culture bed may include regions of varying porosity and/or surface treatments to achieve desired objectives. Related methods are also provided.
A bioreactor includes a fixed bed for culturing cells and a sensor system for sensing a density of the cells in the fixed bed. The sensor may be selected from the group comprising: (a) a sensor for measuring impedance across at least a portion of the fixed bed; (b) a flowmeter for detecting a rate of flow of liquid associated with the fixed bed; (c) a sensor for measuring a pressure differential in a flow of liquid through the fixed bed; (d) a monitor, such as a light sensor or microscope, for detecting light from a light source for projecting light on or in the fixed bed, wherein the detected or measured characteristic is indicative of cell density in the fixed bed; (e) a chemical sensor within the fixed bed for detecting a chemical indicative of cell density in the fixed bed. Related sensor arrangements, systems, and methods are also disclosed.
A bioreactor includes a housing with a number of removable parts for modular construction and easy assembly or disassembly. The removable parts include a fixed bed and a support for the fixed bed. More than one support may be provided. The multiple supports may interlock with each other or a lid of the bioreactor. The support interlocks with the housing to prevent relative rotation. The multiple supports include a support frame for supporting the fixed bed an allowing fluid to flow through the support frame. The fixed bed forms a peripheral chamber between the support and the housing to hold the fixed bed. One or more probes and tubes may be inserted into the bioreactor to test parameters of or to add to or remove liquid from inside.
A cell culturing container including a fixed bed is provided for forming a bioreactor (100). In one version, a container in the form of a tubular body is formed by first and second interlocking portions (115a, 115b). The container forms a compartment for receiving the fixed bed (122) for culturing cells, which container may be removably inserted into an inner compartment of the bioreactor so as form an outer chamber for circulating fluid to or from the fixed bed. The container may also include a removable base (130) for receiving an agitator (118) for agitating and thus circulating the fluid. Related methods are also disclosed.
An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
17.
BIOMASS PREDICTION/ESTIMATION SYSTEM BASED ON PARAMETER SENSING FOR FIXED BED BIOREACTOR AND RELATED METHODS
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
18.
BIOMASS PREDICTION/ESTIMATION SYSTEM BASED ON PARAMETER SENSING FOR FIXED BED BIOREACTOR AND RELATED METHODS
A system for biomass sensing includes a fixed bed bioreactor including a container and a fixed bed disposed within such container. At least one sensor is for sensing one or more parameters representative of biomass in the fixed bed. A controller is adapted for correlating the one or more parameters to an amount of biomass of the fixed bed bioreactor, such as using a correlation model. Related systems and methods are also described.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
19.
BIOPROCESSING WITH IMPROVED MEASUREMENT OF PROCESS PARAMETERS, RELATED APPARATUS AND METHODS
A system includes a bioprocessing vessel formed of a material insulative to a liquid medium when present therein. At least one sensor is for sensing a parameter of the bioprocessing vessel and generating a signal indicative of the parameter. At least ne conductor is adapted to provide electrical communication between the liquid medium in the bioprocessing vessel and an external structure to achieve equipotential for reducing noise in the signal produced by the at least one sensor. Related apparatus and methods are also disclosed.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
20.
SYSTEM AND METHOD FOR THE PRODUCTION OF BIOMOLECULES
The current invention relates to a bioreactor cabinet, configured to be incorporated in a biomolecule production system, wherein said bioreactor cabinet is preferably a mobile bioreactor cabinet suited to receive a bioreactor, said bioreactor cabinet is provided with a bioreactor docking station, wherein said bioreactor cabinet, more preferably a side wall of said bioreactor cabinet, is provided with a connector allowing the transmission of power, signals and/or data when paired with a biomolecule production system, such as a bioreactor chamber of said system. The current invention equally relates to a biomolecule production system and methods for producing biomolecules.
An apparatus for sampling a cell culture includes a sampler for connecting to a bioreactor with a fixed bed including the cell culture. A releasably attached sample portion for positioning within or adjacent the fixed bed includes a substrate on which cells may be grown and then removed from the bioreactor to provide an indication of cell growth. A kit for sampling a cell culture bed of a bioreactor includes the sampler for sampling the cell culture bed with the releasably attached sample portion, along with a container adapted to detach the sample portion from the sampler. A method of obtaining a sample of a cell culture in a bed within a bioreactor includes positioning a sampler positioned at least partially in or adjacent to the bed to promote the growth of cells on a releasably attached portion of the sampler, withdrawing the sampler from the bioreactor, and detaching the portion of the sampler.
An apparatus is provided for culturing and transfecting cells using a liquid. A bioreactor includes a cell culture bed for receiving the liquid. An external loop is connected to the bioreactor and adapted for circulating the liquid to and from the bioreactor. The external loop is adapted for introducing a gas into the liquid therein and/or for circulating a transfection material in the liquid therein to the bioreactor. In one embodiment, a first vessel is associated with the external loop for receiving the liquid, and a gas injector is provided for injecting a gas into the liquid, such as into the first vessel. A second vessel is also provided for providing a transfection material (such as a transfecting agent as part of a transfection mixture) to the liquid, such as directly to the bioreactor or to the external loop. In another embodiment, the gas injector is associated directly with the external loop. The external loop may be dedicated for gas injection and/or transfection, and maintained separate from a loop for circulating liquid media to the bioreactor, which potentially allows for operation on demand. Related methods are also disclosed.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
23.
BIOREACTOR WITH ENHANCED GAS TRANSFER AND THERMAL REGULATION
An apparatus for culturing cells with enhanced gas transfer and thermal regulation is provided. The apparatus includes a bioreactor with a cell culture bed, such as a fixed structured bed. A pump, such as an agitator, serves to pump liquid through the cell culture bed, and a container is provided for the agitator. A first conduit may be associated with the container, such as by being connected to it or adjacent an opening into it. The bioreactor may also include flow extenders to enhance the gas transfer to a liquid used as media to culture the cells, as well as optional thermoregulators. Related methods are also disclosed.
An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
25.
BIOREACTOR SYSTEM WITH ENHANCED CELL HARVESTING CAPABILITIES AND RELATED METHODS
Systems and methods for harvesting cells include a bioreactor with a structure for cell entrapment/adherence and growth, such as a fixed bed structure. A cell harvest mechanism is adapted to agitate the bioreactor, such as by vibrating or shaking the fixed bed, and to move a liquid level relative to the structure, such as by repeatedly flushing and filling the bioreactor. A cell detaching solution is provided to the bioreactor, such as an enzymatic cocktail for the detachment of cells without producing clumps or aggregates. The bioreactor may comprise a preculture vessel upstream of another bioreactor.
A system for producing and purifying target biologics is disclosed. A cell culture unit is provided for generating target biologics in a volume of fluid. A purification unit is also provided for purifying the target biologics in the volume of fluid portion directly from the cell culture unit without a clarifying step, such as by using one or more separators, such as expanded beds or chromatography columns arranged in parallel, and potentially operating in series to achieve continuous operation. The one or more separators may include magnetic beads adapted for binding to the target biologics. The purification unit may receive at least a portion of the volume of fluid directly from the cell culture unit, and a collection unit may be provided for collecting the purified target biologics from the purification unit, and also may be adapted for returning the volume of fluid without the target biologics to the cell culture unit. The system may comprise a first vessel adapted to concentrate a volume of fluid including the target biologics, and a second vessel adapted to further concentrating the volume of fluid, the first or second vessels including magnetic beads adapted for binding with the target biologics. Related methods are also disclosed.
09 - Appareils et instruments scientifiques et électriques
37 - Services de construction; extraction minière; installation et réparation
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Bio manufacturing equipment for laboratory research,
clinical research and trials and for biopharmaceutical
manufacturing industries, namely, containment isolators for
sterility purposes or to prevent contamination, cell culture
vessels, bioreactors for cell culturing, bioreactors for
laboratory use, cabinets in the nature of biological safety
cabinets for cell cultures, cell culture instruments,
namely, incubators for cell culture, equipment for
purification of cell cultures and isolation of target
molecules, equipment for analyzing cell cultures and target
molecules, and equipment for analyzing cell cultures, target
molecules and pharmaceutical preparations, equipment for
packaging in the nature of incubating pharmaceutical
preparations, laboratory centrifuges, filters, bottle top
filters used for the purification of tissue culture media,
laboratory fluidized bed reactors (fbr), storage vessels in
the nature of cell culture dishes, filtration membranes and
process control equipment in the nature of temperature
controllers, concentration meters, control valves and level
switches for monitoring, electrical sensors, oxygen sensors,
biomass sensors, thermistors, temperature sensors, liquid
level sensors, temperature indicators, ultrasonic sensors
and ultrasonic flow detectors, controlling and regulating
the flow of gases or liquids, all forming an integrated
platform and for the viral vaccine production; replacement
parts of the aforesaid goods; laboratory equipment for
bioprocessing, namely, self-contained bio manufacturing
chambers containing environmental control and containment
barriers for use in safe handling of biological materials,
also for use in manufacturing of biologics, none of which
are for medical purposes; bioreactors for cell culturing;
disposable plastic bioreactors for cell culturing. Installation and maintenance of biopharmaceutical
manufacturing equipment; advisory services relating to the
installation and maintenance of biopharmaceutical
manufacturing equipment. Engineering, scientific and technological consultancy on
process, namely, product development relating to the
manufacture of biologics; engineering, scientific and
technological consultancy on production units for the
development and production of biologics, namely, product
development; design of tools for the production of
biologics; advisory services relating to industrial
engineering in the field of manufacturing of biologics;
biochemical engineering services; technical engineering in
the field of manufacturing of biologics; rental of
laboratory apparatus and instruments; product and
pharmaceutical research and development for the
pharmaceutical and biotechnology industries; custom design
and development of chemical reagents; analytical testing
services, namely, materials evaluation testing of
pharmaceutical, biopharmaceutical and chemical products,
featuring testing for safety, toxicity, content, identity,
purity, configuration and function; providing counseling and
information with regard to aforementioned services.
The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
An apparatus for culturing cells includes a bioreactor including a monolithic structured cell culture bed, which may be fabricated using additive manufacturing techniques, such as for example 3D printing. The bioreactor and monolithic structured cell culture bed may be formed as a unitary structure. The monolithic structured cell culture bed may include regions of varying porosity and/or surface treatments to achieve desired objectives. Related methods are also provided.
A cell culturing container including a fixed bed is provided for forming a bioreactor (100). In one version, a container in the form of a tubular body is formed by first and second interlocking portions (115a, 115b). The container forms a compartment for receiving the fixed bed (122) for culturing cells, which container may be removably inserted into an inner compartment of the bioreactor so as form an outer chamber for circulating fluid to or from the fixed bed. The container may also include a removable base (130) for receiving an agitator (118) for agitating and thus circulating the fluid. Related methods are also disclosed.
A bioreactor includes a fixed bed for culturing cells and a sensor system for sensing a density of the cells in the fixed bed. The sensor may be selected from the group comprising: (a) a sensor for measuring impedance across at least a portion of the fixed bed; (b) a flowmeter for detecting a rate of flow of liquid associated with the fixed bed; (c) a sensor for measuring a pressure differential in a flow of liquid through the fixed bed; (d) a monitor, such as a light sensor or microscope, for detecting light from a light source for projecting light on or in the fixed bed, wherein the detected or measured characteristic is indicative of cell density in the fixed bed; (e) a chemical sensor within the fixed bed for detecting a chemical indicative of cell density in the fixed bed. Related sensor arrangements, systems, and methods are also disclosed.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
32.
SYSTEM, APPARATUS AND METHOD FOR THE PRODUCTION OF CELLS AND/OR CELL PRODUCTS
The present invention provides a system and a method for the production of cells and/or cell products. The system comprises at least one cell culture unit comprising at least one bioreactor for culturing cells, at least one technical control unit for controlling a cell growth parameters, said technical control unit is at least fluidly connected to the cell culture unit, and at least one air treatment unit for treating ambient air, said air treatment unit is fluidly connected to the cell culture unit. The system is characterized in that the system is autonomous and the bioreactor total volume is at most 1000L.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
33.
SYSTEM AND METHOD FOR THE PRODUCTION OF BIOMOLECULES
The current invention relates to a bioreactor cabinet, configured to be incorporated in a biomolecule production system, wherein said bioreactor cabinet is preferably a mobile bioreactor cabinet suited to receive a bioreactor, said bioreactor cabinet is provided with a bioreactor docking station, wherein said bioreactor cabinet, more preferably a side wall of said bioreactor cabinet, is provided with a connector allowing the transmission of power, signals and/or data when paired with a biomolecule production system, such as a bioreactor chamber of said system. The current invention equally relates to a biomolecule production system and methods for producing biomolecules.
The current disclosure concerns a method for decontaminating a virus production system, wherein virus particles are produced in at least one bioreactor and purified from liquids comprising said virus particles thereby generating liquid waste, said method comprising the step of exposing said system or a part thereof to heated air and/or liquid at a temperature of at least 60° C. after viral production took place, thereby eradicating remaining virus particles in said system. In a second and third aspect the current disclosure concerns a virus production system wherein the system comprises a heating device suitable for heating air and/or liquid to a temperature of at least 60° C., and the use of such a system for the production of viruses and/or viral vaccines.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
A61L 2/04 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des phénomènes physiques de la chaleur
35.
Bioprocessing with improved measurement of process parameters, related apparatus and methods
A system includes a bioprocessing vessel formed of a material insulative to a liquid medium when present therein. At least one sensor is for sensing a parameter of the bioprocessing vessel and generating a signal indicative of the parameter. At least one conductor is adapted to provide electrical communication between the liquid medium in the bioprocessing vessel and an external structure to achieve equipotential for reducing noise in the signal produced by the at least one sensor. Related apparatus and methods are also disclosed.
An apparatus is provided for bioprocessing, such as for culturing cells. The apparatus includes a bioreactor with a chamber having cells and a chamber for) temperature regulation of the cells. In some embodiments, a freezer is connected to the bioreactor for freezing the cells in the chamber, and a heater may also be provided for actively thawing the cells. Related methods are also disclosed.
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
37.
BIOREACTOR WITH ENHANCED GAS TRANSFER AND THERMAL REGULATION
An apparatus for culturing cells with enhanced gas transfer and thermal regulation is provided. The apparatus includes a bioreactor with a cell culture bed, such as a fixed structured bed. A pump, such as an agitator, serves to pump liquid through the cell culture bed, and a container is provided for the agitator. A first conduit may be associated with the container, such as by being connected to it or adjacent an opening into it. The bioreactor may also include flow extenders to enhance the gas transfer to a liquid used as media to culture the cells, as well as optional thermoregulators. Related methods are also disclosed.
The current disclosure concerns a system for producing biomolecules comprising a docking station (30), said docking station encompasses: —a bioreactor (1); —a concentrator (2) fluidly linked to the bioreactor; —an intermediate vessel (4), positioned between said bioreactor (1) and concentrator (2), wherein said intermediate vessel (4) and concentrator (2) are connected by a retentate conduit (303), allowing recirculating of liquid from an output of the concentrator to an input of said intermediate vessel and—a controller, integrated in said docking station (30), which is able to control the biomolecule process.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.
The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retentate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.
An apparatus is provided for bioprocessing, such as for culturing cells. The apparatus includes a bioreactor with a chamber having cells and a chamber for temperature regulation of the cells. In some embodiments, a freezer is connected to the bioreactor for freezing the cells in the chamber, and a heater may also be provided for actively thawing the cells. Related methods are also disclosed.
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
43.
System for the production of cells and/or cell products
The invention provides a method and a system for the production of viruses or virus derived products. The system comprises a containment enclosure provided with a single entry means and a single exit means which are movable between a closed position and an open position. The containment enclosure comprises at least one production unit comprising at least one bioreactor for culturing the virus, said bioreactor is provided with at least one inlet and at least one outlet; at least one purification unit fluidly connected to the production unit and comprising at least one purification means having at least one inlet and at least one outlet. The system further comprises at least one inactivation and/or at least one gas decontamination unit. The containment enclosure comprises at least one process control device connected to the exit means. The movement of the exit means from the closed position to the open position is controllable by said process control device.
An apparatus for culturing cells includes a bio reactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed beds arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side -to -side and align with an external drive. Related methods are also disclosed.
The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retentate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.
09 - Appareils et instruments scientifiques et électriques
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Bio manufacturing equipment for laboratory research,
clinical research and trials and for (bio)pharmaceutical
manufacturing industries, namely, isolators, cell culture
vessels, cabinet for cell culture, cell culture instruments,
handling vessels, filtration membranes and process control
equipment; parts of the aforesaid goods; equipment for
bioprocessing, namely, self-contained bio manufacturing
chambers containing environmental control and containment
barriers for use in safe handling of biological materials,
also for use in manufacturing of biologics. Custom manufacture of biologics, including manufacturing of
biologics using biological organisms in the manufacturing
process. Engineering, scientific and technological consultancy on
process development relating to manufacture of biologics;
engineering, scientific and technological consultancy on
production units for the development of biologics; design of
tools for the production of biologics; advisory services
relating to industrial engineering in the field of
manufacturing of biologics; biochemical engineering
services; technical engineering in the field of
manufacturing of biologics; rental of laboratory apparatus
and instruments; research and development for the
pharmaceutical and biotechnology industries.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Bioreactors for cell culturing; bioreactors for laboratory
research, clinical research and trials and for
(bio)pharmaceutical manufacturing industries; disposable
plastic bioreactors for cell culturing.
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Bioreactor for cell culturing; Bioreactors for laboratory research, clinical research and trials and for (bio)pharmaceutical manufacturing industries; Disposable plastic bioreactors for cell culturing
09 - Appareils et instruments scientifiques et électriques
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Biomanufacturing equipment for laboratory research, clinical research and trials for (bio)pharmaceutical manufacturing industries, namely, isolators, cell culture vessels, cabinet for cell culture, cell culture instruments, handling vessels, filtration membranes and process control equipment, parts of the aforesaid goods; Equipment for bioprocessing, namely, self-contained biomanufacturing chambers containing environmental control and containment barriers for use in safe handling of biological materials, also for use in manufacturing of biologics (1) Custom manufacture of biologics, including manufacturing of biological organisms in the manufacturing process
(2) Engineering, scientific and technological consultancy on process development relating to manufacture of biologics; engineering, scientific and technological consultancy on production units for the development of biologics; design of tools for the production of biologics; advisory services relating to industrial engineering in the field of manufacturing of biologics; biochemical engineering services; technical engineering in the field of manufacturing of biologics; rental of laboratory apparatus and instruments; research and development for the pharmaceutical and biotechnology industries
The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
51.
System, apparatus and method for biomolecules production
An automated method for the production of cells and/or biomolecules such as protein or peptides includes culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The total volume of the bioreactor is at least 10 liters. A system suitable for implementation of the automated method above is a small-scale and cupboard-sized system, which can be placed in a portable clean room.
C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/16 - Appareillage pour l'enzymologie ou la microbiologie contenant ou adaptés pour contenir des milieux solides
C12M 1/40 - Appareillage spécialement destiné à l'utilisation d'enzymes libres, immobilisées ou liées à un support, p. ex. appareils contenant un lit fluidisé d'enzymes immobilisées
