Provided is a method for increasing the kernel row number of an ear of corn, increasing the width of the ear of corn, increasing corn kernel weight or improving corn yield. The method comprises the step of mutating the CLE gene in corn. The mutation of the CLE gene can lead to increases in the kernel row number of an ear of corn, ear width, kernel weight and yield thereof. Therefore, the method has important application value in corn breeding.
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
The present invention belongs to the field of nucleic acid editing, and particularly to the technical field of clustered regulatory interspaced short palindromic repeats (CRISPR). Specifically, provided in the present invention is an adenosine deaminase, which is fused with a DNA binding protein and can be used for single-base editing of a target nucleic acid, and has wide application prospects.
The present invention provides a mutated acetyl-CoA carboxylase (ACC) protein, a nucleic acid, and a use thereof. Specifically, the present invention relates to a mutated ACC protein, a nucleic acid, and a use thereof in plant breeding. Specifically, the present invention provides a mutated ACC protein, and compared with a parent ACC protein, the mutated ACC protein is mutated at the 1878th amino acid and/or the 1879th amino acid corresponding to the amino acid sequence represented by SEQ ID No. 1, or is mutated at the 1791st amino acid and/or the 1792nd amino acid corresponding to the amino acid sequence represented by SEQ ID No. 3. Plants having a mutated ACC have high herbicide resistance, and have wide prospects of application in the cultivation of herbicide-resistant plants.
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/46 - Gramineae ou Poaceae, p. ex. ivraie, riz, blé ou maïs
A br2 mutant protein, a CDS sequence and a 3'UTR sequence of the mutant br2 protein having base deletions relative to the CDS sequence and the 3'UTR sequence of a parent br2 protein, and the parent br2 protein being derived from corn. The br2 mutant protein can cause reduced plant height and ear height in corn, improved lodging resistance and increased yield under high-density planting conditions, and has important application value in dwarf corn breeding.
Provided are a mutant acetolactate synthase (ALS) polypeptide and a use thereof. Specifically, provided is a mutant ALS, wherein the mutant polypeptide comprises mutant ALS1 and mutant ALS3, and the mutant ALS1 polypeptide is mutated at position 178 of SEQ ID No. 1 compared to a parent ALS1 polypeptide, and/or the mutant ALS3 polypeptide is mutated at position 172 of SEQ ID No. 2 compared to a parent ALS3 polypeptide. The mutated ALS polypeptide has strong tolerance to herbicides, and has broad application prospects in the fields of improving plant tolerance to ALS-inhibiting herbicides and cultivating plants with tolerance to ALS-inhibiting herbicides.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12N 9/78 - Hydrolases (3.) agissant sur les liaisons carbone-azote autres que les liaisons peptidiques (3.5)
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/54 - Leguminosae ou Fabaceae, p. ex. soja, luzerne ou arachide
Provided is a Cas enzyme, which belongs to the field of nucleic acid editing, specifically the technical field of clustered regularly interspaced short palindromic repeats (CRISPR), and has wide application prospects in the field of gene editing.
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
C12N 15/65 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression utilisant des marqueurs
7.
CAS12J PROTEIN HAVING IMPROVED EDITING ACTIVITY AND USE THEREOF
The present application relates to the field of nucleic acid editing, in particular to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, provided are a Cas12j mutant protein having improved editing activity and the use thereof. The present application has wide prospects of application.
The present invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, provided in the present invention is an engineered V-type Cas protein.
The present invention belongs to the field of plant genetic engineering. Specifically provided are a mutated PYL polypeptide and the use thereof. Compared with a parent PYL polypeptide, the mutated PYL polypeptide has a mutation at the 147th amino acid site corresponding to SEQ ID No.1. The plant yield can be improved by means of expressing the mutated polypeptide in plants. The mutated PYL polypeptide has wide application prospects.
C07K 14/415 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de végétaux
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
10.
CAS PROTEIN WITH IMPROVED EDITING ACTIVITY AND USE THEREOF
A Cas mutant protein. Compared with a parent Cas protein, the Cas mutant protein has a significantly improved editing activity and broad application prospects.
The present invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of clustered regularly interspaced short palindromic repeats (CRISPRs). Specifically, the present invention provides a mutated Cas12j protein and use thereof, and the mutated Cas12j protein has wide application prospects.
The present invention belongs to the field of nucleic acid editing, and particularly to the technical field of clustered regularly interspaced short palindromic repeats (CRISPRs). Specifically, the present invention provides a mutated Cas-sf0005 protein and use thereof, having wide application prospects.
The present invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, provided are an optimized Cas protein and the use thereof, and the optimized Cas protein has a wide use prospect.
The present invention relates to the field of nucleic acid editing, and in particular to the technical field of clustered regularly interspaced short palindromic repeats (CRISPRs). Specifically, the present invention provides a novel Cas enzyme. The novel Cas enzyme has relatively low homology with reported Cas enzymes, can show the activity of nuclease inside and outside cells, and has a wide application prospect.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
C12N 15/65 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression utilisant des marqueurs
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
The present invention relates to the field of nucleic acid editing, and in particular, to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR); and specifically provides an optimized Cas protein and the use thereof, which have wide application prospects.
The present invention relates to the field of nucleic acid editing, and in particular, to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, the present invention provides a novel Cas enzyme. The Cas enzyme is Cas-sf9149, has relatively low homology with reported Cas enzymes, can show nuclease activity in cells and outside cells, and has wide application prospects.
Provided is a novel Cas enzyme, which has a relatively low homology to reported Cas enzymes. The Cas enzyme exhibits nuclease activity intracellularly and extracellularly, and is prospective in applications in the field of nucleic acid editing, particularly in the technical field of clustered regularly interspaced short palindromic repeats (CRISPR).
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
Provided are a novel Cas enzyme. The Cas enzyme is Cas-sf0005, Cas-sf9417, Cas-sf8553 or Cas-sf1846, has low homology with reported Cas enzymes, can exhibit nuclease activity inside cells and outside cells, and has a wide application prospect.
Provided are a herbicide-resistant gene, a polypeptide, and an application thereof in plant breeding. Specifically, provided is a mutant HPPD polypeptide, and compared with a parent HPPD polypeptide, the mutant HPPD polypeptide has mutation occurred at any one or more of amino acids corresponding to positions 54, 133, and 401 of SEQ ID NO.1. The mutant HPPD polypeptide is highly tolerant of herbicides, and has a wide application prospect in the field of improving and cultivating plants tolerant of anti-HPPD inhibitory herbicides.
Provided are a mutant type acetyl-CoA carboxylase (ACC) protein, a nucleic acid and use thereof in plant breeding. The mutant type acetyl-CoA carboxylase (ACC) protein, compared with a parent acetyl-CoA carboxylase (ACC) protein, has mutations at any one or more of the following amino acid positions corresponding to the amino acid sequence shown in SEQ ID NO: 1: position 2125, position 2097, position 2139, position 2194, position 2186, position 2273, position 2168, position 1975, position 1954, position 1864, position 2211, position 2187, position 2123, and position 2126; and acetyl-CoA carboxylase (ACC) mutated plants have high herbicide resistance, and can be cultivated as herbicide-resistant plants.
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A mutant granule-bound starch synthase 1 (GBSS1) polypeptide and a nucleic acid, and use thereof are provided. Compared to an amino acid sequence of a parent GBSS1, the mutant GBSS1 polypeptide has a mutation at an amino acid corresponding to one or more of amino acid 237, amino acid 168, and amino acid 411 of an amino acid sequence shown in SEQ ID NO: 1. An amylose content (AC) in a plant changes after the plant undergoes GBSS1 mutation, which has very promising application prospects in the improvement of edible quality of rice.
A CRISPR-associated (Cas) protein, a fusion protein including the Cas protein, and a nucleic acid encoding either of the proteins are provided. The Cas protein is any one from the group consisting of a Cas protein having an amino acid sequence with at least 95% sequence identity with SEQ ID NO: 1 and basically retaining a biological function of SEQ ID NO: 1; a Cas protein having an amino acid sequence obtained through a substitution, a deletion, or an addition of one or more amino acids based on SEQ ID NO: 1 and basically retaining the biological function of SEQ ID NO: 1; and a Cas protein comprising an amino acid sequence shown in SEQ ID NO: 1.
Provided is a novel Cas enzyme, which has low homology to the reported Cas enzymes, can exhibit the activity of nuclease both inside and outside cells, and has application prospects in the field of nucleic acid editing, especially in the technical field of clustered regularly interspaced short palindromic repeats (CRISPR).
C07K 14/195 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
A mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid encoding the mutant ACC protein, and use thereof are provided. Specifically, compared with a parent ACC protein, the mutant ACC protein has mutations at amino acids corresponding to amino acid 1,879 and/or amino acid 2,186 of SEQ ID NO: 1. An ACC-mutated plant shows high herbicide resistance, and thus the present disclosure has very promising application prospects in the cultivation of an herbicide-resistant plant.
A mutant granule-bound starch synthase 1 (GBSS1) polypeptide and a nucleic acid, and use thereof are provided. Compared to an amino acid sequence of a parent GBSS1, the mutant GBSS1 polypeptide has a mutation at an amino acid corresponding to amino acid 427 and/or amino acid 428 of an amino acid sequence shown in SEQ ID NO: 1. An amylose content (AC) in a plant changes after the plant undergoes GBSS1 mutation, which has very promising application prospects in the improvement of edible quality of rice.
A method for multiplex nucleic acid detection based on clustered regularly interspaced short palindromic repeat (CRISPR), a system, and a kit for detecting a target nucleic acid based on CRISPR are provided. The detection method includes: adding any one, any two, any three, or four from the group consisting of a first nucleic acid detection composition, a second nucleic acid detection composition, a third nucleic acid detection composition, and a fourth nucleic acid detection composition to a reaction system with a target nucleic acid to achieve the multiplex detection of the target nucleic acid.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
The present invention provides an expression regulatory element and a use thereof, and relates in particular to an expression regulatory element and a use thereof in regulating the expression of one or more genes in a cell. Further disclosed is a use of an expression regulatory element in plant breeding. Further disclosed is a method for improving the expression of a target gene by using an expression regulatory element of the invention. The method comprises: operably linking an expression regulatory element of the present invention to a target gene, so as to improve the expression of the target gene.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12N 15/67 - Méthodes générales pour favoriser l'expression
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leur taxonomie botanique
A01H 6/20 - Brassicaceae, p. ex. colza, brocoli ou roquette
A01H 6/82 - Solanaceae, p. ex. poivron, tabac, pomme de terre, tomate ou aubergine
A01H 6/46 - Gramineae ou Poaceae, p. ex. ivraie, riz, blé ou maïs
A01H 6/54 - Leguminosae ou Fabaceae, p. ex. soja, luzerne ou arachide
A01H 6/74 - Rosaceae, p. ex. fraise, pomme, amande, poire, rose, mûre ou framboise
A01H 6/02 - Amaranthaceae or Chenopodiaceae, p. ex. betterave ou épinard
30.
HERBICIDE-RESISTANT ACETYL-COENZYME A CARBOXYLASE MUTANT AND APPLICATION THEREOF
A mutant acetyl-coenzyme A carboxylase (ACC) protein, a nucleic acid, and an application thereof, which relate to a mutant ACC protein, a nucleic acid, and an application thereof in plant breeding. Specifically provided is a mutant ACC protein. Comparing the mutant ACC protein with the parent ACC protein, a mutation occurs at an amino acid corresponding to position 1879 and/or position 2186 in SEQ ID NO:1. Plants in which ACC is mutated have high herbicide resistance and have very broad application prospects in the cultivation of herbicide-resistant plants.
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
Provided is a method for enhancing vitamin-C content in plants using a GGP2 and/or GGP1 genome 5'-terminal untranslated region (5'-UTR) nucleotide mutant in plant cells. The mutant has a fragment deletion of 50-80bp compared to a parent 5'-UTR nucleotide. The sequence of the parent 5'-UTR nucleotide is shown in SEQ ID NO:1. The fragment deletion comprises a sequence shown in SEQ ID NO:2.
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leur taxonomie botanique
32.
METHOD FOR MULTIPLEX NUCLEIC ACID DETECTION BASED ON CRISPR TECHNOLOGY
The present invention provides a method, system, and kit for target nucleic acid detection based on a CRISPR technology. The method comprises: contacting a sample with a nucleic acid detection composition, wherein the nucleic acid detection composition comprises a Cas protein, a gRNA, and a single-stranded nucleic acid detector, and the gRNA comprises a region that binds to the Cas protein and a guide sequence that hybridizes to a target sequence on a target nucleic acid; and detecting a detectable signal generated by cleaving the single-stranded nucleic acid detector by the Cas protein, thereby detecting the target nucleic acid.
Provided are a method, system and kit for performing target nucleic acid detection by using a single-stranded nucleic acid detector comprising abasic spacers. The detection method comprises: adding gRNA, a Cas protein and a single-stranded nucleic acid detector to a reaction system comprising a target nucleic acid, the single-stranded nucleic acid detector comprising at least one arbitrary nucleotide and at least one abasic spacer.
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
34.
POLYPEPTIDE AND NUCLEIC ACID CAPABLE OF CHANGING CONTENT OF AMYLOSE IN PLANTS AND USE THEREOF
Provided in the present invention are a mutant GBSS1 polypeptide, a nucleic acid and the use thereof, and the present invention specifically relates to the mutant GBSS1 polypeptide, the nucleic acid and the use thereof in plant breeding. The content of amylose in plants is changed by means of GBSS1 mutation, therefore the present invention has a very wide application prospect in improving the edible quality of Oryza sativa.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A method for detecting a target nucleic acid using a nucleic acid analogue or a base modification, a system and a kit. The detection method comprises the step of adding a gRNA, a Cas protein and at least one detector selected from a single-stranded nucleic acid analog detector and a single-stranded nucleic acid detector having a base modification to a reaction system containing a target nucleic acid.
Provided are a method and system for carrying out target nucleic acid detection using a novel Cas enzyme, and particularly provided are a method, system and kit for detecting whether a characteristic sequence to be detected exists in a target nucleic acid or not by using a novel Cas enzyme. The detection method comprises: adding an exonuclease, a gRNA, a Cas protein and a single stranded nucleic acid detector into a reaction system containing the target nucleic acid. The method can effectively shorten the detection time and has higher sensitivity.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Provided is a method for enhancing plant aroma, and further provided are a use of inhibitors of a BADH2 gene or an encoded protein thereof for enhancing plant aroma; or preparing a composition or preparation for enhancing plant aroma, the BADH2 gene comprising BADH2a and BADH2b genes. Inhibiting the expression of a BADH2 gene or an encoded protein thereof can significantly enhance plant aroma, especially that of corn.
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
38.
METHOD FOR DETECTING TARGET NUCLEIC ACID ON BASIS OF CRISPR TECHNOLOGY
Provided in the present invention is a method for detecting a target nucleic acid on the basis of CRISPR technology, and specifically, provided are a method, a system and a kit for detecting the target nucleic acid on the basis of CRISPR technology. The detection method comprises adding a gRNA, a Cas protein and a single-stranded nucleic acid detector into a reaction system containing the target nucleic acid, wherein the single-stranded nucleic acid detector can be single-stranded DNA, single-stranded RNA or a single-stranded DNA-RNA hybrid.
Provided are a nucleic acid expression method and a nucleic acid construct. The nucleic acid construct driven by a specific promoter realizes gRNA-guided efficient base-directed mutation in plants.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12N 9/78 - Hydrolases (3.) agissant sur les liaisons carbone-azote autres que les liaisons peptidiques (3.5)
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
Provided are a fusion protein and an application thereof. Specifically, provided is a fusion protein, which comprises a component selected from among the following, or is composed of the following components: (1) a positioning functional element D1, which has the function of targeting and binding DNA; and (2) a demethylated functional element D2, which has the function of converting a methylated nucleotide into an unmethylated nucleotide.
Provided are an herbicide-resistant gene, a polypeptide of the genetic code, and an application thereof in plant breeding; the polypeptide is a mutated ALS polypeptide, in a wild type ALS polypeptide as shown in SEQ ID NO: 1, the amino acid at position 170 and/or 627 has mutated, imparting herbicide tolerance, which can be used to cultivate plants tolerant to ALS inhibitor herbicides.
Provided are a herbicide-resistant polypeptide, a nucleic acid and the use thereof. Specifically, provided are a herbicide-resistant gene, a polypeptide and the use thereof in plant breeding. Specifically, provided is a mutant HPPD polypeptide, and compared with the parent HPPD polypeptide, the mutant HPPD polypeptide has a mutation of the amino acid at position 347 and/or of the amino acid at position 353 corresponding to SEQ ID NO.1. The mutated HPPD polypeptide is highly tolerant of herbicides, and has a wide application prospect in the field of improving and cultivating plants with tolerance to an anti-HPPD inhibitory herbicide.
Provided is an application of a photorespiratory branch protein in regulation of plant traits. Specifically, provided are a reagent combination and a fusion protein. The reagent combination or the fusion protein comprises glycolate oxidase or a coding nucleic acid thereof, and/or oxalate oxidase or a coding nucleic acid thereof, and/or catalase or a coding nucleic acid thereof. Importing the reagent combination or the fusion protein into plant cells can significantly regulate agronomic traits of plants.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
Provided is the use of a substance, used for improving the properties of plants or for preparing preparations or compositions for regulating the shape of plants, said plant traits comprising one or more traits selected from the following group: (i) root length, root branching, and/or root weight; (ii) plant height; and (iii) salt tolerance; said substance is selected from the following group: (a) HAK protein; (b) a nucleic acid sequence encoding the HAK protein; (c) HAK protein and its encoding nucleic-acid sequence promoter; or a combination thereof. The HAK protein or mutated protein thereof and corresponding method can achieve the regulation of lodging resistance, biomass, salt resistance, and other traits, and has important scientific value for cultivating high-quality, high-yield, and stress-tolerant new plant varieties.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leur taxonomie botanique
45.
APPLICATION OF TPST GENE IN REGULATION OF TRAITS OF PLANT
Provided is an application of a tyrosylprotein sulfotransferase (TPST) gene or an encoded protein thereof or a promoter thereof in the regulation of traits of a plant or in the preparation of a preparation or composition for regulating traits of a plant. Increasing the level of expression or the activity of the TPST gene or the encoded protein thereof in a plant may improve the traits of the plant, the traits of the plant comprising one or more traits selected from among the following groups: (i) stress resistance; (ii) thousand kernel weight; (iii) yield and/or biomass; and (iv) the size, weight and/or number of fruit and/or seeds.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/46 - Gramineae ou Poaceae, p. ex. ivraie, riz, blé ou maïs
46.
APPLICATION OF MIR396 OR CODING GENE MUTANT THEREOF
Provided is an application of miR396 or a coding gene mutant thereof. Also provided are a method for increasing the nitrogen fertilizer utilization rate and/or reducing the application amount of nitrogen fertilizer for plants, a composition comprising a miR396 inhibitor and use thereof, a method for producing gene-edited plant tissues or plant cells or plants, and a method for producing a plant having a high nitrogen utilization rate.
Provided is the use of an miR396 or a mutant of an encoding gene thereof in regulating an agronomic trait of a plant. Also provided are the use of a GRF gene or an accelerator of an encoding protein thereof, a method for improving an agronomic trait of a plant, a composition for improving an agronomic trait of a plant under low nitrogen conditions and a use thereof, a method for preparing gene-edited plant tissue or a gene-edited plant cell, and a method for preparing a gene-edited plant.
Provided are a mutant gene editing protein and a fusion protein containing the mutant gene editing protein. The mutant gene editing protein and the fusion protein can identify more types of PAM sequences so as to expand a gene editing range, improve the gene editing efficiency and accurately mediate mutation of a target site, and can be widely applied to animal and plant cells.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/20 - Brassicaceae, p. ex. colza, brocoli ou roquette
A01H 6/82 - Solanaceae, p. ex. poivron, tabac, pomme de terre, tomate ou aubergine
49.
HERBICIDE-RESISTANT GENE, POLYPEPTIDE, AND APPLICATION THEREOF IN PLANT BREEDING
Provided is a mutant HPPD polypeptide, having one or more differences in amino acid sequence compared with a parent HPPD polypeptide. The difference comprises a mutation occurring at the 342nd amino acid corresponding to SEQ ID NO.:1, and the mutant HPPD polypeptide has strong tolerance to herbicides. Also provided are a coding gene of the mutant HPPD polypeptide and an application thereof in plant breeding.
Provided is a plant improvement method, wherein knocking out N members (N ≥ 1) of a miR156 gene family can improve seed dormancy and enhance the capability of pre-harvest sprouting prevention of seeds. Also provided are a genetically engineered plant tissue or plant cell and a preparation method therefor, a method for preparing a genetically engineered plant, a grain production method, and use of a miR156 gene family inhibitor.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/46 - Gramineae ou Poaceae, p. ex. ivraie, riz, blé ou maïs
Provided is a nucleic acid construct for gene editing, wherein the nucleic acid construct having a specific structure can permit gRNA-directed site-directed mutagenesis of bases in plants.
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
A01H 6/46 - Gramineae ou Poaceae, p. ex. ivraie, riz, blé ou maïs
A01H 6/54 - Leguminosae ou Fabaceae, p. ex. soja, luzerne ou arachide
A01H 6/20 - Brassicaceae, p. ex. colza, brocoli ou roquette
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